Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,093,346 (T)6→5 coding (471/1359 nt) dgcT → putative diguanylate cyclase DgcT

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,093,3460T.96.0% 103.1 / ‑3.2 25coding (471/1359 nt)dgcTputative diguanylate cyclase DgcT
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base . (16/8);  total (17/8)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTGATTTACTTTGTTATAACCGTCATTATCATTCAGCAAATTATTGAGGAGCGTTTGACAAGCAGTGTTGTCCAAAATGACATTGCAATCTATTATTTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCAAAAAAATGACTTTGTGCATAAGTTTGTTTTTTGTTTTTGGGGGGCCGATAGTTGCTCACATACTTTCTAGTCATTATGAGAGCTACAATTTACACATTGCCGAGTTAACCAATGAAAATGGTCAGGTGGTTTGGAAAGCCTCATATGTTACCATAATGATTTTCATGTGGTTAACCTTGCTTTCGGTTAATCTATACTTTAATGGTTTACGCTATGATATCTGGAATGGAGTGACGGTGATTGCTTTTTGTGCCGTACTGTACAATATCTCTCTGTTATT  >  NC_000913/1093128‑1093594
                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                        
ttGATTTACTTTGTTATAACCGTCATTATCATTCAGCAAATTATTGAGGAGCGTTTGACAAGCAGTGTTGTCCAAAATGACATTGCAATCTATTATTTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCAAAAAAATGACTTTGTGCATAAGTTTGTTTTT‑GTTTTTGGGGGGCCGATAGTTGCTCACATACtt                                                                                                                                                                                                                         >  1:52744/1‑251 (MQ=255)
  gATTTACTTTGTTATAACCGTCATTATCATTCAGCAAATTATTGAGGAGCGTTTGACAAGCAGTGTTGTCCAAAATGACATTGCAATCTATTATTTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCAAAAAAATGACTTTGTGCATAAGTTTGTTTTT‑GTTTTTGGGGGGCCGATAGTTGCTCACATACTTTc                                                                                                                                                                                                                       <  1:114836/251‑1 (MQ=255)
           tGTTATAACCGTCATTATCATTCAGCAAATTATTGAGGAGCGTTTGACAAGCAGTGTTGTCCAAAATGACATTGCAATCTATTATTTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCAAAAAAATGACTTTGTGCATAAGTTTGTTTTT‑GTTTTTGGGGGGCCGATAGTTGCTCACATACTTTCTAGTCAtt                                                                                                                                                                                                               <  2:52744/250‑1 (MQ=255)
                       cATTATCATTCAGCAACTTATTGAGGAGCGTTTGACAAGCAGTGTTGTCCAAAATGACATTGCAATCTATTATTTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCAAAAAAATGACTTTGTGCATAAGTTTGTTTTT‑GTTTTTGGGGGGCCGATAGTTGCTCACATACTTTCTAGTCATTATGAGAGCTACa                                                                                                                                                                                                   >  2:107524/1‑250 (MQ=255)
                               ttCAGCAAAGTATTGAGGAGCGTTTGACAAGCAGAGTTGTCCAAGATGACAATGCAATCGATTATTTGTGTCGTCCGATGAGTTGGGGCATCTTACTATTTCTGGCATTGGAGACTAAAGTTAGTGAAAACACAAAACAACGCAATTTATTTTACAAACAAATGAATGTGTGCATAAGATTGTGTTTTGTTTT‑GGGGGACCGATAGTTGCTCACATACTTTCTAGTCATTATGAGAGCTACAATTTacac                                                                                                                                                                                           >  2:434261/1‑250 (MQ=255)
                                                 agCGATTGAAAAGCCGTGTTGTCCAAAGTAACAAATCAATCTATTATTTGTTAAGTCAAATGAGTTTGAGCATATTAATATTTGTGGCATTGGTGAATAAAGATAGTGAAAACACAAAACAGCGCAATTTATTTTACAAAAAAATGACTATGTACATAAGTTTGTTTTT‑GTTTTTGGTGGGCAAATAGATGCTAACATATTTTCTAGTCTGTATGAGAACAACCATTTAAACATTGcctatttaatcatgg                                                                                                                                                                        >  2:482952/1‑238 (MQ=255)
                                                   cGTTTGACAAGCAGTGTTGTCCAAAATGACATTGCAATCTATTATTTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCAAAAAAATGACTTTGTGCATAAGTTTGTTTTT‑GTTTTTGGGGGGCCGATAGTTGCTCACATACTTTCTAGTCATTATGAGAGCTACAATTTACACATTGCCGAGTTAACCAATGa                                                                                                                                                                       >  1:189025/1‑250 (MQ=255)
                                                         aCAAGCAGTGTTGTCCAAAATGACATTGCAATCTATTATTTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCAAAAAAATGACTTTGTGCATAAGTTTGTTTTT‑GTTTTTGGGGGGCCGATAGTTGCTCACATACTTTCTAGTCATTATGAGAGCTACAATTTACACATTGCCGAGTTAACCAATGAAAATGGt                                                                                                                                                                >  1:225315/1‑251 (MQ=255)
                                                                   ttgtCCAAAATGACATTGCAATCTATTATTTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCAAAAAAATGACTTTGTGCATAAGTTTGTTTTT‑GTTTTTGGGGGGCCGATAGTTGCTCACATACTTTCTAGTCATTATGAGAGCTACAATTTACACATTGCCGAGTTAACCAATGAAAATGGTCAGGTGGttt                                                                                                                                                      >  1:510393/1‑251 (MQ=255)
                                                                       ccAAAATCCCATTGCCATCTATTATTTGTTTCGTCAGATGCGTTCGTGCATATTAATCTTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCAAAAAACTGACTTTGTGCATAAGTTTGTTTTT‑GTTTTTGGGGGGCCGATATTTGCTCACATACTTTCTAGTCATTATGAGAGCTACAATTTACACATTGCCGAGTTAACCAATGAAAATGGTCAGGTGGTTTGGa                                                                                                                                                   >  2:291106/1‑250 (MQ=255)
                                                                                               tttGTCTCGTCAGATGAGATTATGGATATTATTATTTCTGTCATTGGTGCATAAAGTTCGTGAAGACACAAAACAGAGCAATTTCTTTTCAAAAAAAATGAGTTTGTGAAAACGTTTGTTTTT‑GTTTTTAGAGGGCAGAAAGTTGCTCACATACTTTCTAGTCATTATGAGAGCTACAATTTACACATTGAAGTGTTAACAAATGAACATGGTCAGGTGGTTTGGAAAGCCTCATATGTTACCATAATGAt                                                                                                                          >  2:482538/1‑251 (MQ=255)
                                                                                                               aGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAACGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCAAAAAAAATGACTTTGTGCATAAGTTTGTTTTT‑GTTTTTGGGGGGCCGATAGTTGCTCACATACTTTCTAGTCATTATGAGAGCTACAATTTACACATTGCCGAGTTAACCAATGAAAATGGTCCGGTGGTTTGGAAAGCCTCATATGTTACCATAATGATTTTCATGTGGTTAAcc                                                                                                          >  2:488998/1‑251 (MQ=255)
                                                                                                                   tgagCATATTAATATTTCTGGCATTGGTGGATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCAAAAAAATGACTTAGTGCATAAGTTTGTTTTT‑GTTTTTAGGAGGCCGATAGTTGCTCAACTACTTTCTAGTCATTATGAGAGCTACAATTTACACATTGCCTAGTTAACCAATGCAAATGGTAAGGTAGTTTGGAAAGCCTCATATGTTACCATAATGATTTTAATGTGGTTAACCTTGc                                                                                                      >  2:257866/4‑251 (MQ=255)
                                                                                                                        atatTAATATTTCTGGCATTGGAGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCAAAAAAATGACTTTGTGCATAAGTTTGTTTTT‑GTTTTTGGGGGGCCGATAGTTGCTCACATACTTTCTAGTCATTATGAGAGCTACAATTTACACATTGCCGAGTTAACCAATGAAAATGGTCAGGTGGTTTGGAAAGCCTCATATGTTACCATAATGATTTTCATGTGGTTAACCTTGCTTTCg                                                                                                 >  1:189600/1‑251 (MQ=255)
                                                                                                                            tAATATTTCTGTCATTGGTGAATAAAGGTAGTGAAAACACAAAACAGCGCAATTTATTTTCCAAAAAAATGACTTTGTGCATAAGTTTGTTTTT‑GTTTTGGGGGGGCCGATAGTTGCTCACATACTGTCTAGTCATTATGAGAGCTACAATTTACACATTGCCGAGTTAACCAATGAAAATTGTCAGGTGGTTTGGAAAGCCTCATATGTGACCAGAATGAGGTTCATGTTGTTAACCTTGCGTTCGGtt                                                                                              <  2:442668/250‑1 (MQ=255)
                                                                                                                              atatTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCAAAAAAATGACTTTGTGCATAAGTTTGTTTTT‑GTTTTTGGGGGGCCGATAGTTGCTCACATACTTTCTAGTCATTATGAGAGCTACAATTTACACATTGCCGAGTTAACCAATGAAAATGGTCAGGTGGTTTGGAAAGCCTCATATGTTACCATAATGATTTTCATGTGGTTAACCTTGCTTTCGGTTAAt                                                                                           <  1:475611/251‑1 (MQ=255)
                                                                                                                                          ttGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCAAAAAAATGACTTTGTGCATAAGTTTGTTTTT‑GTTTTTGGGGGGCCGATAGTTGCTCACATACTTTCTAGTCATTATGAGAGCTACAATTTACACATTGCCGAGTTAACCAATGAAATTGGTCAGGTGGTTTGGAAAGCCTCATATGTTACCATAATGATTTTCATGTGGTTAACCTTGCTTTCGGTTAATCTATACTTTAAt                                                                               >  2:181349/1‑251 (MQ=255)
                                                                                                                                                  tAAAGTTAGTGAAAACACAAAACAGCGCCATTTATTTTCCACAAACATGACTTTTAGAATAAGTTTGTTTTT‑GTTTTTGGGGGGCCGATAGTTGCTCACATACTTTCTAGTCATTATGAGAGCTACAATTTACACATTGCCGAGTTAACCAATGAAAATGGTCAGGTGGTTTGGAAATCATCATATGTTACCATAATGATTTTCATGTGTTTAACCTTGCTTTCGTTTAATCTATACTTTAATGGTTTACg                                                                       >  2:167392/1‑251 (MQ=255)
                                                                                                                                                    aaGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCAAAAAAATGACTTTGTGCATAAGTTTGTTTTT‑GTTTTTGGGGGGCCGATAGTTGCTCACATACTTTCTAGTCATTATGAGAGCTACAATTTACACATTGCCGAGTTAACCAATGAAAATGGTCAGGTGGTTTGGAAAGCCTCATATGTTACCATAATGATTTTCATGTGGTTAACCTTGCTTTCGGTTAATCTATACTTTAATGGTTTACGCt                                                                     <  1:340348/251‑1 (MQ=255)
                                                                                                                                                              aaaCACAAAACAGCGCAATTTATTTTCCAAAAAAATGACTTTGTGCATAAGTTTGTTTTT‑GTTTTTGGGGGGCCGATAGTTGCTCACATACTTTCTAGTCATTATGAGAGCTACAATTTACACATTGCCGAGTTAACCAATGAAAATGGTCAGGTGGTTTGGAAAGCCTCATATGTTACCATAATGATTTTCATGTGGTTAACCGTGCGTTCGGTTAATCTATACTTTAATGGTTTACGCTATGATATCTg                                                           <  2:237595/251‑1 (MQ=255)
                                                                                                                                                                         aGCGCAATTTATTTTCCAAAAAAATGACTTTGTGCATAAGTTTGTTTTT‑GTTTTTGGGGGGCCGATAGTTGCTCACATACTTTCTAGTCATTATGAGAGCTACAATTTACACATTGCCGAGTTAACCAATGAAAATAGTCAGGTGGTTTGGAAAGCCTCATATGTTACCATAATGATTTTCATGTGGTTAACCTTGCTTTCGGTTAATCTATACTTTAATGGTTTACGCTATGATATCTGGAATGGAGTg                                                 >  1:394766/1‑250 (MQ=255)
                                                                                                                                                                                   atttTCCAAAAAAATGACTTTGTGCATAAGTTTGTTTTT‑GTTTTTGGGGGGCCGATAGTTGCTCACATACTTTCTAGTCATTATGAGAGCTACAATTTACACATTGCCGAGTTAACCAATGAAAATGGTCAGGTGGTTTGGAAAGCCTCATATGTTACCATAATGATTTTCATGTGGTTAACCTTGCTTTCGGTTAATCTATACTTTAATGGTTTACGCTATGATATCTGGAATGGAGTGACGGTGATTg                                       <  1:127849/250‑1 (MQ=255)
                                                                                                                                                                                                 tGACTTTGTGCATAAGTTTGTTTTT‑GTTTTTGGGGGGCCGATAGTTGCTCACATACTTTCTAGTCATTATGAGAGCTACAATTTACACATTGCCGAGTTAACCAATGAAAATGGTCAGGTGGTTTGGAAAGCCTCATATGTTACCATAATGATTTTCATGTGGTTAACCTTGCTTTCGGTTAATCTATACTTTAATGGTTTACGCTATGATATCTGGAATGGAGTGACGGTGATTGCTTTTTGTGCCGTAc                        >  1:301924/1‑251 (MQ=255)
                                                                                                                                                                                                      ttGTGCATAAGTTTGTTTTT‑GTTTTTGGGGGGCCGATAGTTGCTCACATACTTTCTAGTCTTTATGAGAGCTACATTTTACACATTGCCGAGTTAACCAATGAAAATGGTCAGGTGGTTTGGAAAGCCTCATATGTTACCATAATGATTTTCATGTGGTTAAc                                                                                                           >  2:171293/1‑163 (MQ=255)
                                                                                                                                                                                                                gtttgtttTT‑GTTTTTGGGGGGCCGATAGTTGCTCACATACTTTCTAGTCATTATGAGAGCTACAATTTACACATTGCCGAGTTAACCAATGAAAATGGTCAGGTGGTTTGGAAAGCCTCATATGTTACCATAATGATTTTCATGTGGTTAACCTTGCTTTCGGTTAATCTATACTTTAATGGTTTACGCTATGATATCTGGAATGGAGTGACGGTGATTGCTTTTTGTGCCGTACTGTACAATAtctctc         <  1:181349/251‑1 (MQ=255)
                                                                                                                                                                                                                        tttgtttttgGGGGGCCGATAGATGCTCACATACTTTCTAGTCATTATGAGAGCTACAATTTACACATTGCCGAGTTAACCAATGAAAATGGTCAGGTGGTTTGGAAAGCCTCATATGTTACCATAATGATTTTCATGTGGTTAACATTGCTTTCGGTTAATCTATACTTTAATGGTTTACGCTATGATATCTGGAATGGAGTGACGGTGATTGATTTTTGTGCCGTACTGTACAATATCTCTCTGttatt  >  2:434161/1‑251 (MQ=255)
                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                        
TTGATTTACTTTGTTATAACCGTCATTATCATTCAGCAAATTATTGAGGAGCGTTTGACAAGCAGTGTTGTCCAAAATGACATTGCAATCTATTATTTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCAAAAAAATGACTTTGTGCATAAGTTTGTTTTTTGTTTTTGGGGGGCCGATAGTTGCTCACATACTTTCTAGTCATTATGAGAGCTACAATTTACACATTGCCGAGTTAACCAATGAAAATGGTCAGGTGGTTTGGAAAGCCTCATATGTTACCATAATGATTTTCATGTGGTTAACCTTGCTTTCGGTTAATCTATACTTTAATGGTTTACGCTATGATATCTGGAATGGAGTGACGGTGATTGCTTTTTGTGCCGTACTGTACAATATCTCTCTGTTATT  >  NC_000913/1093128‑1093594

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑