Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 809,217 2 bp→AG coding (40‑41/1290 nt) bioA ← adenosylmethionine‑8‑amino‑7‑oxononanoate aminotransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913809,2170CA98.3% 193.9 / ‑4.4 59coding (41/1290 nt)bioAadenosylmethionine‑8‑amino‑7‑oxononanoate aminotransferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base A (22/36);  minor base . (0/1);  total (22/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*NC_000913809,2180AG98.3% 199.1 / ‑6.5 59W14R (TGG→CGG) bioAadenosylmethionine‑8‑amino‑7‑oxononanoate aminotransferase
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base G (22/36);  total (22/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTTGCGGCACAGCTCAATGGCTGGCGCATGGGTGATACCGCCAAACATCACATGCGACATGGCATCAATTTGCGACTTCATCGCCGCATTAAGCTGCGGGTGATTGTAGCCGTGGATCGCCGCCCACCAGGACGACATACCGTCAACCAGGCGTCTGCCGTCAGACAAAATCAGCTCGCAACCTTCGGCGCTCACCACCGGATAAACCGGCAGAGGGGAGGTCATGGATGTGTATGGGTGCCAG‑ATATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTTGTAAACCAAATTGAAAAGATTTAGGTTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGCCCCATGGCTCACCGCCCACGCTGGACATTGTCGCAAGTCACAGAATTATTTGAAAAACCGTTGCTGGATCTGCTGTTTGAAGCGCAGCAGGTGCATCGCCAGCATTTCG  >  NC_000913/808976‑809449
                                                                                                                                                                                                                                                 ||                                                                                                                                                                                                                                        
tttGCGGCACAGCTCAATGGCTGGCGCATGGGTGATACCGCCAAACATCACATGCGACATGGCATCAATTTGCGACTTCATCGCCGCATTAAGCTGCGGGTGATTGTAGCCGTGGATCGCCGCCCACCAGGACGACATACCGTCAACCAGGCGTCTGCCGTCAGACAAAATCAGCTCGCAACCTTCGGCGCTCACCACCGGATAAACCGGCAGAGGGGAGGTCATGGATGGGTATGGGTGCAGG‑ATATGGc                                                                                                                                                                                                                                 >  2:418709/1‑251 (MQ=255)
   gCGGCACAGCTCAATGGCTGGCGCATGGGTGATACCGCCAAACATCACATGCGACATGGCATCAATTTGCAACTTCATCGCCGCATTAAGCTGCGGGTGATTGTAGCCGTGGATCGCCGCCCACCAGGACGACATTCCGCCAACCAGGCGTCTGCCGTCAGACAAAATCAGCTCGCAACCTTCGGCGCTCACCACCGGATAAACCGGCAGAGGGGAGGTCATGGATGTGTATGGGTGCAGG‑ATATGGCGt                                                                                                                                                                                                                               <  1:80438/250‑1 (MQ=255)
    cGGCACAGCTCAATGGCTGGCGCATGGGTGATACCGCCAAACATCACATGCGACATGGCATCAATTTGCGACTTCATCGCCGCATTAAGCTGCGGGTGATTGTAGCCGTGGATCGCCGCCCACCAGGACGACATACCGTCAACCAGGCGTCTGCCGTCAGACAAAATCAGCTCGCAACCTTCGGCGCTCACCACCGGATAAACCGGCAGAGGGGAGGTCATGGATGTGTATGGGTGCAGG‑ATATGGCGTTg                                                                                                                                                                                                                             <  2:359923/251‑1 (MQ=255)
      gCACAGCTCAATGGCTGGCGCATGGGTGATACCGCCAAACATCACATGCGACATGGCATCAATTTGCGACTTCATCGCCGCATTAAGCTGCGGGTGATTGTAGCCGTGGATCGCCGCCCACCAGGACGACATACCGTCAACCAGGCGTCTGCCGTCAGACAAAATCAGCTCGCAACCTTCGGCGCTCACCACCGGATAAACCGGCAGAGGGGAGGTCATGGATGTGTATGGGTGCAGG‑ATATGGCGTTGGt                                                                                                                                                                                                                           <  1:437028/251‑1 (MQ=255)
                aTGGCTGGCGCATGGGTGATACCGCCAAACATCACATGCGACATGGCATCAATTTGCGACTTCATCGCCGCATTAAGCTGCGGGTGATTGTAGCCGTGGATCGCCGCCCACCAGGACGACATACCGTCAACCAGGCGTCTGCCGTCAGACAAAATCAGCTCGCAACCTTCGGCGCTCACCACCGGATAAACCGGCAGAGGGGAGGTCATGGATGTGTATGGGTGCAGG‑ATATGGCGTTGGTCAAAGGCatg                                                                                                                                                                                                                 <  2:367461/251‑3 (MQ=255)
                   gctggcGCATGGGTGATACCGCCAAACATCACATGCGACATGGCATCAATTTGCGACTTCATCGCCGCATTAAGCTGCGGGTGATTGTAGCCGTGGATCGCCGCCCACCAGGACGACATACCGTCAACCAGGCGTCTGCCGTCAGACAAAATCAGCTCGCAACCTTCGGCGCTCACCACCGGATAAACCGGCAGAGGGGAGGTCATGGATGTGTATGGGTGCAGG‑ATATGGCGTTGGTCAAAGGCAAGATc                                                                                                                                                                                                              <  2:379745/251‑1 (MQ=255)
                        cgcATGGGTGATACCGCCAAACATCACATGCGACATGGCATCAATTTGCGACTTCATCGCCGCATTAAGCTGCGGGTGATTGTAGCCGTGGATCGCCGCCCACCAGGACGACATACCGTCAACCAGGCGTCTGCCGTCAGACAAAATCAGCTCGCAACCTTCGGCGCTCACCACCGGATAAACCGGCAGAGGGGAGGTCATGGATGTGTATGGGTGCAGG‑ATATGGCGTTGGTCAAAGGCAAGATCGTCCg                                                                                                                                                                                                         <  1:307061/251‑1 (MQ=255)
                          cATGGGTGATACCGCCAAACATCACATGCGACATGGCATCAATTTGCGACTTCATCGCCGCATTAAGCTGCGGGTGATTGTAGCCGTGGATCGCCGCCCACCAGGACGACATACCGTCAACCAGGCGTCTGCCGTCAGACAAAATCAGCTCGCAACCTTCGGCGCTCGCCGCCGGATAATCCGGCAGAGGGGAGGTCATGGATGTGTATGGGTGCAGG‑ATATGGCGTTGGTCAAAGGCAAGATCGTCCGtt                                                                                                                                                                                                       <  1:308188/251‑1 (MQ=255)
                           gtGGGTGATACCGCCAAACATCACAGGCGACATGGCATCAATTTGCGACTTCATCGCCGCATTAAGCTGCGGGTGATTGTAGCCGGGGATCGCCGCCCACCAGGACGACATACCGTCAACCAGGGGTCGGCCGTCAGACAAAATCAGCTCGCAACCTTCGGCGCTCACCGCCGGAGAAACCGGCAGAGGGGAGGTCATGGTTGTGTATGGGTGCAGG‑ATATGGCGTTGGTCAAAGGCAAGATCGTCCGTTg                                                                                                                                                                                                      <  1:324524/250‑1 (MQ=255)
                           aTGGTTGATACCGCCAAACATCACATGCGACATGGCATCAATTTGCGACTTCATCGCCGCATTAAGCTGCGGGTGATTGTAGCCGTGGATCGCCGCCCACCAGGACGACATACCGTCAACCAGGCGTCTGCCGTCAGACAAAATCAGCTCGCAACCTTCGGCGCTCACCACCGGATAAACCGGCAGAGGGGAGGTCATGGATGTGTATGGGTGCAGG‑ATATGGCGTTGGTCAAAGGCAAGATCGTCCGTTg                                                                                                                                                                                                      <  2:498794/251‑1 (MQ=255)
                            tGGGTGATACAGCCAAACATCACATGCGACATGGCATCAATTTGCGACTTCATCGCCGCATTAAGCTGCGGGTGATTGTACCCGTGGATCGCTGACCACCAGGACGACATCACGTCAACCAGGCGTCTGCCGTCAGACCAAATCAGCTCGCAACCTTCGGCGCTCACCACCGGATAAACAGGCAGAGGGGAGGTCATGGATGTGGCTGGGAGCAGG‑ATATGGAGTTGGTCAAAGGCAAGCTCGTCCGTTGt                                                                                                                                                                                                     >  2:303564/1‑251 (MQ=255)
                               gTGATACCGCCAAACATCACATCCGACATGGCCTCAATTTGCGACTGCATCGCCGCATTAAGCTGCGTGTGATTGTAGCCGTGGATCGCCGCCCACCAGTACGACATACCGTCAACCATGCGTCTGCCGTCAGACAAAAGCAGCTCGCAACCTTCGGCGCTCACCTCCGGATAAACCGGCAGAGGGGAGGTTATGGATGTGTATGTGTGCAGG‑ATTTTACGTTTGTCAAAGGCAAGATCGTCCGTTGTCAt                                                                                                                                                                                                  <  2:241886/251‑1 (MQ=255)
                                  aTACCGCCAAACATCACATTCGACATGGCATCAATTTGCGACTTCATCGCCGCATTAAGCTGCGGGTGATTGTAGCCGTGGATCGCCGCCCACCAGGACGACATACCGTCAACCAGGCGTCTGCCGTCAGACAAAATCAGCTCGCAACCTTCGGCGCTCACCACCGGATAAACCGGCAGAGGGGAGGTCATGGATGTGTATGGGTGCAGG‑ATATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAAt                                                                                                                                                                                               >  1:67567/1‑251 (MQ=255)
                                          aaaCATCACATGCGACATGGCATCAATTTGCGACTTCATCGCCGCATTAAGCTGCGGGTGATTGTAGCCGTGGATCGCCGCCCACCAGGACGACATACCGTCAACCAGGCGTCTGCCGTCAGACAAAATCAGCTCGCAACCTTCGGCGCTCACCACCGGATAAACCGGCAGAGGGGAGGTCATGGATGTGTATGGGTGCAGG‑ATATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTTGt                                                                                                                                                                                       <  1:246190/251‑1 (MQ=255)
                                           aaCATCACATGCGACATGGCATCAATTTGCGACTTCATCGCCGCATTAAGCTGCGGGTGATTGTAGCCGTGGATCGCCGCCCACCAGGACGACATACCGTCAACCAGGCGTCTGCCGTCAGACAAAATCAGCTCGCAACCTTCGGCGCTCACCACCGGATAAACCGGCAGAGGGGAGGTCATGGATGTGTATGGGTGCAGG‑ATATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTTGTa                                                                                                                                                                                      <  1:301451/251‑1 (MQ=255)
                                             cATCACATGCGACATGGCATCAATTTGCGACTTCCTCGCCGCATTAAGCTGCGGGTGATTGTAGCCGTGGATCGCCGCCCACCCGGACGACATACCGTCAACCAGGCGTCTGCCGTCAGCCAAAATCAGCTCGCAACCTACGGCGCTCACCACCGGATAAACCGGCAGAGGGGAGGTAATGGATGTGTATGGGTGCAGG‑ATATGGCGTTGGTCACAGGCACGCTCGTCCGTTGTCATACTCGCCTTGTaa                                                                                                                                                                                     >  2:445402/1‑250 (MQ=255)
                                             cATCACAGTCGACATGGCATCAATTGGCGACTTCACCGCCGCATTAAGCTGCGGGTGATTGTAGCCGTGGATCGCCGCCCACCAGGACGACATACCGTCAACCAGGCGTCTGCCGTCAGACAAAATCAGCTCGCAACCTTCGGCGCTCACCACCGGATAAACCTGCAGAGGGGAGGTCATGGAGGTGTATGGGTGCAGG‑ATATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTTGTaaa                                                                                                                                                                                    <  1:321491/251‑1 (MQ=255)
                                              aTCACATGCGACATGGCATCAATTTGCGACTTCATCGCCGCATTAAGCTGCGGGTGATTGTAGCCGTGGATCGCAGCCCACCAGGAAGACATACCGTCAACCCGGCGTCTGCCGTCAGACAAAATCAGCTCGCAACCTTCGGCGCTCACCACCGGATAAACCGGCAGAGGGGAGGTCATGGATGTGTATGGGTGCAGG‑ATATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTTGTAAAc                                                                                                                                                                                   >  1:324447/1‑251 (MQ=255)
                                              aTCACATGCGACATGGCATCAATTTGCGACTTCATCGCCGCATTAAGCGGCGGGTGATTGTAGCCGTGGATCGCCGCCCACCAGGACGACATACCGTCAACCAGGCGTCTGCCGTCAGACAAAATCAGCTCGCAACCTTCGGCTCGCTCCACCGGATAAACCGGCAGAGTGGATGTCATGGATGTGTATGGTTGCAGG‑AGATGTCGTTGGTTAAATGCAAGAGCGGCCGTTGTCATAAGCGACTTTTAAAc                                                                                                                                                                                   <  2:494111/251‑1 (MQ=255)
                                                  caTGCGACATTGCATCAACTTGCGACTGCACCGCCGCATTAAGCAGCGGGTGATTGTAGCCGTGGATCGCCGCCCACCAGGACGACATACCGTCAACCAGGCGTCGGCCGTCAGACAAAATCAGCTCGCAACCTTCGGCGCTCACCACCGGAGAAACCGGCAGAGGGGAGGTCATGGAAGTGTATGGGTGCAGG‑ATATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAAACGACTTGTAAACCaaa                                                                                                                                                                               <  2:272366/251‑1 (MQ=255)
                                                       gACATGGCATCAATTTGCGACTTCAGCGCCGCATTAAGCTGCGGGTGATTGTAGCCGTGGATCGCCGCCCACCAGGACGACATACCGTCAACCAGGCGTCTGCCGTCAGACAAAATCAGCTCGCAACCTTCGGCGCTCACCACCGGATAAACCGGCAGAGGGGAGGTCATGGATGTGTATGGGTGCAGG‑ATATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTTGTAAACCAAATTGaa                                                                                                                                                                          <  2:168279/251‑1 (MQ=255)
                                                          aTGGCATCACTTTGCGACCTCATCGCCGCATTAAGCTGCGGGTGATTGTAGCCGTGGCTAGCCGCCCACCAGGACGACATACCGTCAACCAGGCGTCTGCCGTCAGACAAACTCAGCTCGCAACCTTCGGCGCTCCCCACCGGATAAACCGGCAGCGGGGAGGTCATGGATGTGTATGGGTGCAGG‑ATATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTTGTAAACCAAATTGaaa                                                                                                                                                                         >  2:328015/1‑249 (MQ=255)
                                                             gCATCAATTTGCGACTTCATCGCCGCATTAAGCTGCGGGTGATTGTAGCCGTGGATCGCCGCCCACCAGGACGACATACCGTCAACCAGGCGTCTGCCGTCAGACAAAATCAGCTCGCAACCTTCGGCGCTCACCACCGGATAAACCGGCAGAGGGGAGGTCATGGATGCGTATGGGTGCAGG‑ATATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGCCTTGTAAACCAAATTGAAAAGAtt                                                                                                                                                                    >  2:394960/1‑251 (MQ=255)
                                                              cATCAATTTGCGACTTCATCGCCGCATTAAGCTGCGGGTGATTGTAGCCGTGGATCGCCGCCCACCAGGACGACATACCGTCAACCAGGCGTCTGCCGTCAGACAAAATCAGCTCGCAACCTTCGGCGCTCACCACCGGATAAACCGGCAGAGGGGAGGTCATGGATGTGTATGGGTGCAGG‑ATATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACt                                                                                                                                                                                          >  2:13920/1‑228 (MQ=255)
                                                              cATCAATTTGCGACTTCATCGCCGCATTAAGCTGCGGGTGATTGTAGCCGTGGATCGCCGCCCACCAGGACGACATACCGTCAACCAGGCGTCTGCCGTCAGACAAAATCAGCTCGCAACCTTCGGCGCTCACCACCGGATAAACCGGCAGAGGGGAGGTCATGGATGTGTATGGGTGCAGG‑ATATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACt                                                                                                                                                                                          <  1:13920/228‑1 (MQ=255)
                                                                 aaaTTTGAGACTTCATCGCCGCATTAAGCTGCGGGTGATTGTAGCCGTGGATCGCCGCCCACCAGGACGACATACCGTCAACCAGGCGTCTGCCGTCAGACAAAATCAGATCGCAACCTTCGGCGCTCACCACCGGATAAACCGGCAGAGGGGAGGCCATGGATGTGTATGGGTGCAGG‑CTATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTTGTAAACCAAATTGAAAAGATTTAgg                                                                                                                                                                >  2:15894/2‑251 (MQ=255)
                                                                  aaTTTGCGACTTCATCGCCGCATTAAGCTGCGGGTGATTGTAGCCGTGGATCGCCGCCCACCAGGACGACATACCGTCAACCAGGCGTCTGCCGTCAGACAAAATCAGCTCGCAACCTTCGGCGCTCACCACCGGATAAACCGGCAGAGGGGAGGTCATGGCTGTGTATGGGTGCAGG‑ATATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTTGTAAACCAAATTGAAAAGATTTAGGt                                                                                                                                                               >  1:219801/1‑251 (MQ=255)
                                                                             tCATCGCCGCATTAAGCTGCGGGTGATTGTAGCCGTGGATCGCCGCCCACCAGGACGACATACCGTCAACCAGGCGTCTGCCGTCAGACAAAATCAGCTCGCAACCTTCGGCGCGCACCTCCGGATAAACCGGCAGAGGGGAGGTCATGGATGTGTATGGGTGCAGG‑ATATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAAGCGACTTGTAAACCAAATTGAAAAGATTTAGGTTTACAAGTc                                                                                                                                                      <  1:445402/249‑1 (MQ=255)
                                                                              cATCGCCGCATTAAGCTGCGGGTGATTGTAGCCGTGGATCGCCGCCCACCAGGACGACATACCGTCAACCATGCGTCTGCCGTCAGACAAAATCAGCTCGCAACCTTCGGCGCTCACCACCGGATAAACCGGCAGAGGGGAGGTCATGGATGTGTATGGGTGCAGG‑ATATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTTGTAAACCAAATTGAAAAGATTTAGGTTTACAAGTCTa                                                                                                                                                    <  1:231581/250‑1 (MQ=255)
                                                                                 cgccgcATGAAGCTGCGTGTGATTGTAGCCGTGGATCGCCGCCCACCAGGACGACATACCGTCATCCTGGCGTCTGCCGTCAGACAAGATCAGCTCGCAACCTTCTGCGCTCACCACCGGATAAAGCGGCAGAGGGGAGGGCATAGATGGGGATGGGTGCAGG‑ATGTGTCGTTGGGCAAAGGCTAGATCGTCCTTTGTCATAATCGACTTGTAAACCAAACAGAATAGATTTAGGGTTATAAGGGTACAcc                                                                                                                                                <  2:444784/251‑1 (MQ=255)
                                                                                  gccgcATTAAGCTGCGGGTGATTGTAGCCGTGGATCGCCGCCCACCAGGACGACATACCGTCAACCAGGCGTCTGCCGTCAGACAAAATCAGCTCGCAACCTTCGGCGCTCACCACCGGATAAACCGGCAGAGGGGAGGTCATGGATGTGTATGGGTGCAGG‑ATATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTTGTAAACCAAATTGAAAAGATTTAGGTTTACAAGTCTACACCg                                                                                                                                               >  2:215578/1‑251 (MQ=255)
                                                                                    cgcATTAAGCTGCGGGTGATTGTAGCCGTGGATCGCCGCCCACCAGGACGACATACCGTCAACCAGGCGTCTGCCGTCAGACAAAATCAGCTCGCAACCTTCGGCGCTCACCACCGGATAAACCGGCAGAGGGGAGGTCATGGATGTGTATGGGTGCAGG‑ATATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTTGTAAACCAAATTGAAAAGATTTAGGTTTACAAGTCTACACCGaa                                                                                                                                             <  2:64203/251‑1 (MQ=255)
                                                                                     gcATTAAGCTGCGGGTGATTGTAGCCGTGGATCGCCGCCCACCAGGACGACATACCGTCAACCAGGCGTCTGCCGTCAGACAAAATCAGCTCGCAACCTTCGGCGCTCACCACCGGATAAACCGGCAGAGGGGAGGTCATGGATGTGTATGGGTGCAGG‑ATATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTTGTAAACCAAATTGAAAAGATTTAGGTTTACAAGTCTACACCGAAt                                                                                                                                            >  1:224284/1‑251 (MQ=255)
                                                                                     gcATTAAGCTGCGGGTGATTGTAGCCGTGGATCGCCGCCCACCAGGACGACATACCGTCAACCAGGCGTCTGCCGTCAGACAAAATCAGCTCGCAACCTTCGGCGCTCACCACCGGATAAACCGGCAGAGGGGAGGGCATGGATGTGTATGGGTGCAGG‑ATATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTTGTAAACAAAATTGAAAAGATTTAGGTTTACAAGTCTACACCGAAt                                                                                                                                            >  2:180682/1‑251 (MQ=255)
                                                                                                          tAGCCGTGGATCGCCGCCCACCAGGACGACATACCGTCAACCAGGCGTCTGCCGTCAGACAAAATCAGCTCGCAACCTTCGGCGCTAACCACCGGATAAACCGGCAGAGGGGAGGTCATGGATGTGAATGGGTGCAGG‑ATATGGCGTTGGTCAAGGGCAAGATCGTCCGTTGTCATATTCGACTTGTAAACCAAATTGAAAAGATTTAGGTTTACAAGTCTACACGGAATTAACAACAAAAAACACGtttt                                                                                                                       >  1:322900/1‑251 (MQ=255)
                                                                                                             ccggggATCGCCGCCCACCAGGACGACATACCGTCAACCAGGCGTCTGCCGTCAGACAAAATCAGCTCGCAACCTTCGGCGCTCACCACCGGATAAACCGGCAGAGGGGAGGTCATGGATGTGTATGGGTGCAGG‑ATATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTTGTAAACCAAATTGAAAAGATTTAGGTTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGga                                                                                                                    <  2:426482/247‑1 (MQ=255)
                                                                                                             ccGTGGATCGCCGCCCACCAGGACGACATACCGTCAACCAGGCGTCTGCCGTCAGACAAAATCAGCTCGCAACCTTCGGCGCTCACCACCGGATAAACCGGCAGAGGGGAGGTCATGGATGTGTATGGGTGCAGG‑ATATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTTGTAAACCAAATTGAAAAGATTTAGGTTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTgg                                                                                                                     >  2:236953/1‑250 (MQ=255)
                                                                                                               gTGGATCGCCGCCCACCAGGACGACATACCGTCAACCAGGCGTCTGCCGTCAGACAAAATCAGCTCGCAACCTTCGGCGCTCACCACCGGATAAACCGGCAGAGGGGAGGTCATGGATGTGTATGGGTGCAGG‑ATATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTTGTAAACCAAATTGAAAAGATTTAGGTTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTgg                                                                                                                     >  2:176136/1‑248 (MQ=255)
                                                                                                                          cccACCAGGACGACATACCGTCAACCAGGCGTCTGCCGTCAGACAAAATCAGCCCGCAACCTTCGGCGCTCACCACCGGATAAACCGGCAGAGGGGAGGTCATGGATGTGTATGGGTGCAGG‑ATATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTTGTAAACCAAATTGAAAAGATTTAGGTTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGCCCCATGGc                                                                                                       <  1:348548/251‑1 (MQ=255)
                                                                                                                           ccaccaGGACGACATACCGTCAACCAGGCGTCTGCCGTCAGACAAAATCAGCTCGCAACCTTCGGCGCTCACCACCGGATAAACCGGCAGAGGGGAGGTCATGGATGTGTATGGGTGCAGG‑ATATGGc                                                                                                                                                                                                                                 >  1:118593/1‑128 (MQ=255)
                                                                                                                           ccaccaGGACGACATACCGTCAACCAGGCGTCTGCCGTCAGACAAAATCAGCTCGCAACCTTCGGCGCTCACCACCGGATAAACCGGCAGAGGGGAGGTCATGGATGTGTATGGGTGCAGG‑ATATGGc                                                                                                                                                                                                                                 <  2:118593/128‑1 (MQ=255)
                                                                                                                               caGGACGACATACCGTCAACCAGGCGTCTGCCGTCAGACAAAATCAGCTCGCAACCTTCGGCGCTCACCACCGGATAAACCGGCAGAGGGGAGGTCATGGATGTGTATGGGTGCAGG‑ATATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTTGTAAACCAAATTGAAAAGATTTAGGTTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGCCCCATGGCTCAcc                                                                                                  <  1:418709/251‑1 (MQ=255)
                                                                                                                                   acgacATACTGTCACCCAGGCGTCTGCCGTCAGACAAAATCAGCTTGCAACCTTCGGCGCTAACCACCGGATAAACCGGCAGAGGGGAGGACATGGATGTGTATGGGTGCAGG‑ATATGGCGTTGGTCAAAGGCCAGATAGTCCGTTGTCATAATCGACTTGTAAACCAAATTGAAAAGATTTCGGTTTACCAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGCCCCATGTCTCACCGccc                                                                                              >  2:334626/1‑251 (MQ=255)
                                                                                                                                                                 cAGACAAAATCAGCTCGCAACCTTCGGCGCTCACCACCGGATAAACCGGCAGAGGGGAGGTCATGGATGTGTATGGGTGCAGG‑ATATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTTGTAAACCa                                                                                                                                                                                 <  1:117222/138‑1 (MQ=255)
                                                                                                                                                                 cAGACAAAATCAGCTCGCAACCTTCGGCGCTCACCACCGGATAAACCGGCAGAGGGGAGGTCATGGATGTGTATGGGTGCAGG‑ATATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTTGTAAACCa                                                                                                                                                                                 >  2:117222/1‑138 (MQ=255)
                                                                                                                                                                           cAGCTCGCAACCTTCGGCGCTCACCACCGGATAAACCGGCAGAGGGGAGGTCATGGATGTGTATGGGTGCAGG‑ATATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAAGCGACTTGTAAACCAAATTGAAAAGATTTAGGTTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGTAGAAGCCCCATGGCTCACCGCCCACGCTGGACATTGTCGCAAGTCACAGAATTATTTGaaaa                                                       <  1:85475/250‑1 (MQ=255)
                                                                                                                                                                            aGCTCGCAACCTTCGGAGCTCACCACCGGATAAACCGGCAGAGGGGAGGTCCTGGATGTGTATGGGTGCAGG‑ATATGGCGTTGGTCACAGGCAAGATCGCCCGTTGTCATACTCGACTTGTAAACCAAATTGAAAAGATTTAGGTTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGCCCCCTGGCTCCCCGCCCACGCTGGACATTGTAGCAAGGCACAGAATTCTTTGaaacac                                                     >  1:440399/1‑248 (MQ=255)
                                                                                                                                                                                  cAACCTTCGGCGCTCACCACCGGATAAACCGGCAGAGGGGAGGTCATGGATGTGTATGGGTGCAGG‑ATATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTTGTAAACCAAATTGAAAAGATTTAGGTTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGCCCCATGGCTCACCGCCCACGCTGGACATTGTCGCCAGTCACAGAATTATTTGAAAAACCGTTGc                                               >  2:154459/1‑251 (MQ=255)
                                                                                                                                                                                    aCCTTCGGCGCTCACCACCGGATAAACCGGCAGAGGGGAGGTCATGGATGTGTATGGGTGCAGG‑ATATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTTGTAAACCAAATTGAAAAGATTTAGGTTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGCCCCATGGCTCACCGCCCACGCTGGACATTGTCGCAAGTCACAGAATTATTTGAAAAACCGTTGCTg                                             <  1:105304/251‑1 (MQ=255)
                                                                                                                                                                                     ccctCGGCGCTCACCACCGGATAAACCGGCAGAGGGGAGGTCATGGAAGTGTATGGGTGCAGG‑ATATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTTGTAAACCAAATTGAAAAGATTTAGGTTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGCCCCATGGCTCACCGCCCACGCTGGACATTGTCGCAAGTCACAGAATTATTTGAAAAACCGTTGCTgg                                            <  2:302456/248‑1 (MQ=255)
                                                                                                                                                                                                  ccaccGGATAAACCGGCAGAGGGGAGGTCATGGATGTGTATGGGTGCAGG‑ATATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTTGTACACCAAATTGAAAAGATTTAGGTTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGCCCCATGGCTCACCGCCCACGCTGGACATTGTCGCAAGTCACAGAATTATTTGAAAAACCGTTGCTGGATCTGCTGTTTg                                >  1:502045/1‑250 (MQ=255)
                                                                                                                                                                                                    accGGATAAACCGGCAGAGGGGAGGTCATGGATGTGTATGGGTGCAGG‑ATATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTTGTAAACCAAATTGAAAAGATTTAGGTTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGCCCCATGGCTCACCGCCCACGCTGGACATTGTCGCAAGTCACAGAATTATTTGAAAAACCGTTGCTGGATCTGCTGTTTGAAg                             <  1:174423/251‑1 (MQ=255)
                                                                                                                                                                                                     ccGGATAAACCGGCAGGGGGGAGGTTATGGATGTGGTTGGGTGCAGG‑AAATGGCGTTTGGCAAAAGCAAGATCGGCCGTTGTCATAATCGACTTGTAAACCAAATTGAAAAGAATTAGGTTTACAAGTCTACACCGAATTAACAACAAGAAACACGTGTTGGAGAAGCCCCATGGCTCACCGCCCACGCTGGACATTGTCGCAAGTCACAGAATTATGTGTAAAACCGTTGCTGGAGCTGCTGTTTGAAgc                            <  1:90418/251‑1 (MQ=255)
                                                                                                                                                                                                     ccGGATAAACCGGCAGAGGGGAGGTCATGGATGTGTATGGGTGCAGG‑ATATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTTGTAAACCAAATTGAAAAGATTTAGGTTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGCCCCATGGCTCACCGCCCACGCGGGACATTGTCGCAAGTCACAGAATTATTTTAAAAACCGTTGCGGGATCTGCTGTTTGAAgc                            <  2:322900/251‑1 (MQ=255)
                                                                                                                                                                                                            aaCCGGCAGAGGGGAGGTCATGGATGTGTATGGGTGCAGG‑ATATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTTGTAAACCAAATTGAAAAGATTTAGGTTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGCCCCATGGCTCACCGCCCACGCTGGACATTGTCGCAAGTCACAGAATTATTTGAAAAACCGTTGCTGGATCTGCTGTTTGAAGCGcagcag                     <  1:273409/251‑1 (MQ=255)
                                                                                                                                                                                                             aCCGGCAGAGGGGAGGTCATGGATGTGTATGGGTGC‑AGTATATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTTGTAAACCAAATTGAAAAGATTTAGGTTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGCCCCATGGCTCACCGCCCACGCTGGACATTGTCGCAAGTCACAGAATTATTTGAAAAACCGTTGCTGGATCTGCTGTTTGAAGCGCAGCAgg                    <  1:368978/251‑1 (MQ=255)
                                                                                                                                                                                                                       gggAGGTCATTGATGTGTATGGGTGCAGG‑ATATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTTGTAAACCAAATGGAAAAGATTTAGGTTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGCCCCATGGCTCACCGCCCACGCTGGACATTGGCGCAAGTCACAGAATTAGTTTAAAAACCGTTGCTGGATCGGCTGTTTGAAGCGCAGCAGGTGCATCGCCa          <  2:146722/251‑1 (MQ=255)
                                                                                                                                                                                                                              cATGGATGTGTATGGGTGCAGG‑ATATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTTGTAAACCAAATTGAAAAGATTTAGGTTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGCCCCATGGCTCACCGCCCACGCTGGACATTGTCGCAAGTCACAGAATTATTTGAAAAACCGTTGCTGGATCTGCTGTTTGAAGCGCAGCAGGTGCATCGCCAGCATTTc   <  1:239940/251‑1 (MQ=255)
                                                                                                                                                                                                                               aTGGATGTGTATGGGTGCAGG‑ATATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTTGTAAACCAAATTGAAAAGATTTAGGTTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGCCCCATGGCTCACCGCCCACGCTGGACATTGTCGCAAGTCACAGAATTATTTGAAAAACCGTTGCTGGATCTGCTGTTTGAAGCGCAGCAGGTGCATCGCCAGCATTTCg  <  1:428623/251‑1 (MQ=255)
                                                                                                                                                                                                                                                 ||                                                                                                                                                                                                                                        
TTTGCGGCACAGCTCAATGGCTGGCGCATGGGTGATACCGCCAAACATCACATGCGACATGGCATCAATTTGCGACTTCATCGCCGCATTAAGCTGCGGGTGATTGTAGCCGTGGATCGCCGCCCACCAGGACGACATACCGTCAACCAGGCGTCTGCCGTCAGACAAAATCAGCTCGCAACCTTCGGCGCTCACCACCGGATAAACCGGCAGAGGGGAGGTCATGGATGTGTATGGGTGCCAG‑ATATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTTGTAAACCAAATTGAAAAGATTTAGGTTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGCCCCATGGCTCACCGCCCACGCTGGACATTGTCGCAAGTCACAGAATTATTTGAAAAACCGTTGCTGGATCTGCTGTTTGAAGCGCAGCAGGTGCATCGCCAGCATTTCG  >  NC_000913/808976‑809449

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑