Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 480,295 2 bp→AA coding (13‑14/219 nt) hha ← hemolysin expression‑modulating protein Hha

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913480,2950GA95.0% 47.5 / NA 20P5F (CCT→TTT) hhahemolysin expression‑modulating protein Hha
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (9/10);  minor base C (1/0);  total (10/10)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.54e-01
*NC_000913480,2960GA100.0% 62.4 / NA 20P5F (CCT→TTT) hhahemolysin expression‑modulating protein Hha
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (10/10);  total (10/10)

GCTCCGAAAGCGAATTATTTATTAGCGAATAAATTTCCATACTGAGGAAGGGATCTTGTCGTACAGTTTATTCATGGTCAATTCGGCGAGGCGGTGATCTGCGGCTGAGTAAAATACCGCCAGTTCATTATCTGATAATTCGTATTTATTTTTCTCGATAACACGCTCCAGCGTGTCAATTGTCTGGCAACGACGTAAACGCATTAAATAATCGGTTTTCGTTAAAGGTTTTTCGGACATACTTCTACCTATGGTTGTAATAATTCTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAGATCCTGCATATTAATACCATAACTACTGAACAACATAAAGGTGTCATCCAGATATTCGTCGATCTGCTCAATGAGCTTATTGTCTTCATTATACTTAATTTTGTAATTAAGT  >  NC_000913/480069‑480532
                                                                                                                                                                                                                                  ||                                                                                                                                                                                                                                            
gcgccGAAAGCGAAGTATTTATTAGCTAATAAATTTCCATACTGAGGAAGGGATCTTGTCGTACAGTTTATTCATGGTCAATTCGGCGAGGCGGTGACCTGCGGCTGAGTAAAATACCGCCAGTTCATTATCTGATAATTCGTATTTATTTTTCTCGATAACACGCTCCAGCGTGTCAATTGTCTGGCAACGACGTAAACGCATTAAATAATCGGTTTTCGTTAAAAATTTTTCGGACATACTTCTACCTa                                                                                                                                                                                                                       <  1:293427/248‑1 (MQ=255)
  tCCGAAAGCGAATTATTTATTAGCGAATAAATTTCCATACTGAGGAAGGGATCTTGTCGTACAGTTTATTCATGGTCAATTCGGCGAGGCGGTGATCTGCGGCTGAGTAAAATACCGCCAGTTCATTATCTGATAATTCGTATTTATTTTTCTCGATAACACGCTCCAGCGTGTCAATTGTCTGGCAACGACGTAAACGCATTAAATAATCGGTTTTCGTTAAAAATTTTTCGGACATACTTCTACCTATg                                                                                                                                                                                                                     >  1:274190/1‑251 (MQ=255)
   ccGAAAGCGAATAATTTATTAGCGAATAAATTTCCATACGGAGGAAGGTATTTTGTCGTACAGTTTATTCATGGTCAATTCGGCGAGGCGGTGATCTGCGGCTGAGTAAAATGCCGCCAGTTCATTATCTGATAATTCGTATTTATTTTTCTCGATAACACGCTCCAGCGTGTCAATTGTCGGGCAACGACGTAAACGCATTAAATAATCGGTTTTCGTTAAAAATTTTTCGGACATACTTCTACCTATgg                                                                                                                                                                                                                    <  2:470338/251‑1 (MQ=255)
         gCGAATTATTTATTAGCGAATAAATTTCCATCCTGAGGAAGGGATCTTGTCGTACAGTTTATTCATGGTCAATTCGGCGAGGCGGTGATCTGCGGCTGAGTAAAATACCGCCAGTTCATTATCTGGTTATTCGTATTTATTTTTCTCGATAACACGCCCCAGCGTGTCAATTTTCTGGCAACGACGTAAACGCCTTAAATAATAGGTTTTCGTTAAAAATTTTTCGGACCAACTTCTACCTATGGTTGta                                                                                                                                                                                                               >  2:440754/1‑250 (MQ=255)
                                  ttCCATACTGAGGAAGGGATCTTGTCGTACAGTTTATTCATGGTCAATTCGGCGAGGCGGGGATCTGCGGCTGAGTAACATACCGCCAGTTCATTATCTGATAATTCGGATTTATTTTTCTCTATAACACGCTCCAGCGTGTCAATTGTCTGGCAACGACGTAAACGCCTTAAATAATCGGTTTTCTTTAAAAATTTTTCGGACATACTTCTTACTATGGTTGTAATAATTCTAACAAGCTAAACCCGc                                                                                                                                                                                       >  2:277955/1‑249 (MQ=255)
                                     cttACTGAGGAAGGGATCTTGTCGTACAGTTTATTCATGGTCAATTCGGCGAGGCGGTGATCTGCGGCTGAGTAAAATACCGCCAGTTCATTATCTGATAATTCGTATTTATTTTTCTCGATAACACGCTCCAGCGTGTCAATTGTCTGGCAACGACGTAAACGCATTAAATAATCGGTTTTCGTTAAAAATTTTTCGGACa                                                                                                                                                                                                                                   <  2:379825/200‑1 (MQ=255)
                                            aGGAAGGGATCTTGTCGTACAGTTTATTCATGGTCAATTCGGCGGGGCGGTGATCTGCGTCTGAGTAAAATACCGCCAGTTCATTATCTGATAATTCGTATTTATTTTTCTCGATAACACGCTCCAGCGTGTCAATTGTCTGGCAACGACGTAAACGCATTAAATAATCGGTTTTCGTTAAAAATTTTTCGGACATACTTCTACCTATGGTTGTAATAATTCTAACAAGATAAACTCGCAGGATTCTCttt                                                                                                                                                                           <  1:189420/251‑1 (MQ=255)
                                                                     aTTCATGGTCAATTCGGCGAGGCGGTGCTCTGCGGCTGAGTAAAATAACGACAGTTCAATATCTGATAATTCGTCTTTCTTTTTCTCGATAACACGCTCCCGCGTGTCAATTGTCTGGCAACGACGTAAACGCATTAAATAATCGGTTTTCGTTAAAAATTTTTCGGAAATACTTCTACCTATGGTTGTAATAATTCTAAAAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAAt                                                                                                                                                  >  2:75675/1‑251 (MQ=255)
                                                                                           gggTGTTCTGCGGCTGAATAAAATACCGCCAGTTCAGTATCTGATAATTCGTATTTATTTTTCTCGATAACACGCTCCAGCGTGTCAATTGTCTGGCAACGACGTAAACGCATTAAATAATCGGTTTTCGTTAAAAATTTTTCGGACATACTTCTACCTATGGTTGTAATAATTCTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGcc                                                                                                                            <  2:197649/250‑1 (MQ=255)
                                                                                                               aaaTACCGCCAGTTCATTATCTGATAATTCGTATTTATTTTTCTCGATAACACGCTCCAGCGGGTCAATTGTCTGGCAACGACGTAAACGCATTAAATAATCGGTTTTCGTTAAAAATTTTTCGGACATACTTCTACCTATGGTTGTAATAATTCTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAGATCCTGCata                                                                                                        <  1:440754/251‑1 (MQ=255)
                                                                                                                     cGCCAGTTCATTATCTGATAATTCGTATTTATTTTTCTCGATAACACGCTCCAGCGTGTCAATTGTCTGGCAACGACGTAAACGCATTAAATAATCGGTTTTCGTTAAAAATTTTTCGGACATACTTCTACCTATGGTTGTAATAATTCTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAGATCCTGCATATTAATa                                                                                                  <  1:400540/251‑1 (MQ=255)
                                                                                                                         aGTTCATTATCTGATAATTCGTCTTTATTTTTCTCGATAACACGCTCCAGCGTGTCAATTGTCTGGCAACGACGTAAACGCATTAACTAATCGGTTTTCGTTAAACATTTTTCGGACATACTTCTACCTATGGTTGTAATAATTCTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAGATCCTGCATATTAATACCAt                                                                                              >  2:236187/1‑251 (MQ=255)
                                                                                                                                                           cGATAACACGCTCCAGCGTGTCAATTGTCTGGCAACGACGTAAACGCATTAAATAATCGGTTTTCGTTAAAAATTTTTCGGACATACTTCTACCTATGGTTGTAATAATTCTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAGATCCTGCATATTAATACCATAACTACTGAACAACATAAAGGTGTCATCCAg                                                               <  1:245884/248‑1 (MQ=255)
                                                                                                                                                                                    tgtctgtcGACGCCGTAAAAGCATTATATAATCGGTTTTCGTTAAAAATTTTTCGGACATACCTCTAATTATGGTTGTAATAATTCTAACAAGATAAACTCGCCGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCAGCCATTTATGATGATCCGGCATATTAATACAATAACTACTGAACAACATAAAGGAGGCATCCAGGTATTCGTCGATCTGCTCAATGAGCtt                                    >  2:51173/1‑250 (MQ=255)
                                                                                                                                                                                              cgacgTAAACGCATTAAATAATCGGTTTTCGTTAAAAATTTTTCGGACATACTTCTACCTATGGTTGTAATAATTCTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAGATCCTGCATATTAATTCCATAACTACTGAACAACATAAAGGTGTCATCCAGATATGCGTCGATCTGCTCAATGAGCTTATTGTCTTCAt                         <  2:44133/251‑1 (MQ=255)
                                                                                                                                                                                               gacgTAAACGCATTAAATAATCGGTTTTCGTTAAAAATTTTTCGGACATACTTCTACCTATGGTTGTAATAATTCTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAGATCCTGCATATTAATACCATAACTACTGAACAACATAAAGGTGTCATCCAGATATTCGTCGATCTGCTCAATGAGCTTATTGTCTTCAtt                        >  2:148537/1‑251 (MQ=255)
                                                                                                                                                                                                 cgTAAACGCATTAAATAATCGGTTTTCGTTAAAAATTTTTCGGACATACTTCTACCTATGGTTGTAATAATTCTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTcc                                                                                                                               >  1:487464/1‑146 (MQ=255)
                                                                                                                                                                                                 cgTAAACGCATTAAATAATCGGTTTTCGTTAAAAATTTTTCGGACATACTTCTACCTATGGTTGTAATAATTCTAACAAGATAAACTCGCAGGATTCGCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTcc                                                                                                                               <  2:487464/146‑1 (MQ=255)
                                                                                                                                                                                                     aaCGCATTAAATAATCGGTTTTCGTTAAAAATTTTTCGGACATACTTCTACCTATGGTTGTAATAATTCTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAGATCCTGCATATTAATACCATAACTACTGAACCACATAAAGGTGTCATCCAGATATTCGTCGCTCTGCTCAATGAGCTTATTGTCTTCATTATACtt                  >  1:224669/1‑251 (MQ=255)
                                                                                                                                                                                                                     gatattCGTTAAAAATTTCTCGAACATACTTCTACATATGGTTGTAATAATTCTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGATAAAACAAACGAATAGACGATTACCTTACTTCCGCCATTTCTGAAGATCCTGCATATTAATACCATAACTACTGAACAACATAAAGGTGTCATCCAGATATTCTTCGATCTGCTCAATGAGCTTATTGTCTTCATTATACTTAATTTTGTAATTAAGt  >  2:391267/5‑251 (MQ=255)
                                                                                                                                                                                                                                  ||                                                                                                                                                                                                                                            
GCTCCGAAAGCGAATTATTTATTAGCGAATAAATTTCCATACTGAGGAAGGGATCTTGTCGTACAGTTTATTCATGGTCAATTCGGCGAGGCGGTGATCTGCGGCTGAGTAAAATACCGCCAGTTCATTATCTGATAATTCGTATTTATTTTTCTCGATAACACGCTCCAGCGTGTCAATTGTCTGGCAACGACGTAAACGCATTAAATAATCGGTTTTCGTTAAAGGTTTTTCGGACATACTTCTACCTATGGTTGTAATAATTCTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAGATCCTGCATATTAATACCATAACTACTGAACAACATAAAGGTGTCATCCAGATATTCGTCGATCTGCTCAATGAGCTTATTGTCTTCATTATACTTAATTTTGTAATTAAGT  >  NC_000913/480069‑480532

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: