Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,510,238	T→G	intergenic (+445/+452)	insO → / ← fecE	IS911B regulator fragment/ferric citrate ABC transporter ATP binding subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,510,238	0	T	G	97.4%	121.1 / NA	38	intergenic (+445/+452)	insO/fecE	IS911B regulator fragment/ferric citrate ABC transporter ATP binding subunit
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base G (19/18);  total (20/18)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.65e-01

CTCTGGCGAAAAAGGGAGAAGGACGGGCCCGTCTGCCTTCACTTTATGCCAGCTTCGGTAAGCTGGGTGAGACACCGACTCATGAAGATATCATTGATAACAATCGATCCATCAACTGGCCTGTATAACGTGGCTGCCGGTGATTAAGAAAGCTGCACCTACCTAAGTAGTAGCAAACGCACACTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGT‑TCCATGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTAAACTTAGTCATGTCACGTTTTTGGGTTTCTAAAATTTTAACTTCGCGTTTTTCGTTGCCGTAAGGGTTATACAGAAATGTCCGTTAAGCAGAGTTCAAAATTGAT  >  NC_000913/4510006‑4510477                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                ctctGGCGAAAAAGGGAGAAGGACGGGCCCGTCTGCCTTCACTTTATGCCAGCTTCGGTAAGCTGGGTGAGACACCGACTCATGAAGATATCATTGATAACAATCGATCCATCAACTGGCCTGTATAACGTGGCTGCCGGTGATTAAGAAAGCTGCACCTACCTAAGTAGTAGCAAACGCACACTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGT‑TCCAGGGTTGCTTTTGATTTACg                                                                                                                                                                                                                               >  1:225901/1‑251 (MQ=255)         aaaaaggacgaggaaagggCCCGCCTGACTTCAATTTATGCCAGATTCGGTAAGCTGGGTGCGACACCGACTCCTGCAGATATCATTGATAACAATCGATCCATCACCTGGCCTGTATAACGTGGTTGCCGGTGATTAAGAAAGATGCAACTACCTAAGTAGTAGCAAACGCAAACTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCAGT‑TCCAGGGTTGCTTTTGATTTACGTTGGCGTc                                                                                                                                                                                                                       >  2:293041/17‑251 (MQ=255)           aaaaggAGAAGGACGGGCCCGGCTGCCCTCACTTTATGCCAGCTTCGGGAAGCTGGGTGAGACACCCACTCATTAAGATATCATTGATAACAAACGAACCATCAACTGGCCTGTATAACGTGGCTGCCGGTGATTAAGAAAGCTGCACCTACCTAAGTAGGAGCAAACGCACCCTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGT‑TCCAGGGTTGCTTTTGATTTACGTTGGCGTCTg                                                                                                                                                                                                                     <  2:58391/247‑1 (MQ=255)               ggAGAAGGACGGGCCCGTCTGCCTTCACTTTATGCCAGCTTCGGTAAGCTGGGTGAGACACCGACTCATGAAGATATCATTGATAACAATCGATCCATCAACTGGCCTGTATAACGTGGCTGCCGGTGATTAAGAAAGCTGCACCTACCTAAGTAGTAGCAAACGCACACTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGT‑TCCAGGGTTGCTTTTGATTTACGTTGGCGTCTGATCa                                                                                                                                                                                                                 <  1:228939/251‑1 (MQ=255)                 agaAGGACGGGCCCGTCTGCCTTCACTTTATGCCCGCTTCGGTAAGCTGGGTGAGACACCGACTCATGAAGATATCATTGATAACAATCGATCCATCAACTGGCCTGTATAACGTGGCTGCCGGTGATTAAGAAAGCTGCACCTACCTAAGTAGTAGCAAACGCACACTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGT‑TCCAGGGTTGCTTTTGCTTTACGTTGGCGTCTGATCAtt                                                                                                                                                                                                               >  2:9710/1‑251 (MQ=255)                 agaAGGACGGGCCCGTCTGCCTTCACTTTATGCCAGCTTCGGTAAGCTGGGTGAGACACCGACTCATGAAGATATCATTGATAACAATCGATCCATCAACTGGCCTGTATAACGTGGCTGCCGGTGCTTAAGAAAGCTGCACCTACCTAAGTAGTAGCAAACGCACACTTTTTAGAAAAATCGATGGTCAGACACTGGATTAGCAATTCCGT‑TCCAGGGTTGCTTTTGATTTACGTTGGCGTCTGATCAtt                                                                                                                                                                                                               >  1:434724/1‑251 (MQ=255)                 agaAGGACGGGCCCGTCTGACTTCAGTTTATGCCAGCTTCGGTAAGCTGGGTGAGACACCGACTCATGAAGATATCATTGATAACAATCGATCCATCAACTGGCCTGTATAACGTGGCTGCCGGTGATTAAGAAAGCTGCACCTACCTAAGTAGTAGCAAACGCACACTTTTTAGAAAAATCGATGGTCAGAAACTGGATTGGCAATTCCGT‑TCAAGGGTTGCTTTTGATTTAAGTTGGCGTCTGATCAtt                                                                                                                                                                                                               >  2:162512/1‑251 (MQ=255)                     ggACGGGCCCGTCTGCCTTCACTTTATGCCAGCTTCGGTAAGCTGGGAGAGACACCGACTCATGAAGATATCATTGATAACAATCGATCCATCAACTGGCCTGTATAACGTGGCTGCCGGTGATTAAGAAAGCTGCACCTACCTAAGTAGTAGCAAACGCACACTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGT‑TCCAGGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGAtt                                                                                                                                                                                                           >  1:369448/1‑251 (MQ=255)                                   gCCTTCACTTTATGCCAGCTTCGGTAAGCTGGGGGAGACACCGTCTCATGAAGATATCATTGATAACAATCGATCCATCAACTGGCCTGTATAACGTGGCTGCCGGTGATTAAGAAAGCTGCACCTACCTAAGTAGTAGCAAACGCACACTTTTTAGAAAAAGCGATGGGCAGAAACTGTATTAGCAATTCCGT‑TCCAGGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAg                                                                                                                                                                                               <  1:444217/249‑1 (MQ=255)                                    ccTTCACTTTATGCCAGCTTCGGTAAGCTGGGTGAGACACCGACTCATGAAGATATCATTGATAACAATCGATCCATCAACTGGCCTGTATAACGTGGCTGCCGGTGATTAAGAAAGCTGCACCTACCTAAGTAGTAGCAAACGCACACTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGT‑TCCAGGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGccc                                                                                                                                                                                            <  1:165741/251‑1 (MQ=255)                                    ccTTCACTTTATGCCAGCTTCGGTAAGCTGGGTGAGACACCGACTCATGAAGATATCATTGATAACAATCGATCCATCAACTGGCCTGTATAACGTGGCTGCCGGTGATTAAGAAAGCTGCACCTACCTAAGTAGTAGCAAACGCACACTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGT‑TCCAGGGTTGCTTTTGATTTACGGTGGCGTCTGATCATTGATTTATCCGCAAAAGccc                                                                                                                                                                                            <  1:387265/251‑1 (MQ=255)                                            ttATGCCAGCTTCGGTAAGCTGGGTGAGACACCGACTCATGAAGATATCATTGATAACAATCGATCCATCAACTGGCCTGTATAACGTGGCTGCCGGTGATTAAGAAAGCTGCACCTACCTAAGTAGTAGCAAACGCACACTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGT‑TCCAGGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTc                                                                                                                                                                                      <  1:345507/249‑1 (MQ=255)                                            ttATGCCAGCGTCTGTAAGCTGGGTGAGACAACGACTCATGAAGATATCATTTATAACAATCGATCCATCAACTGGCCTGTATAACGTGGCTGCCGGTGATTAAGAAAGCTGCATCTACCTAAGTAGTAGCAAACGCTCACTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGT‑TCCAGGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTAGCCTCAAAAGCCCAACCTCa                                                                                                                                                                                     <  1:299887/250‑1 (MQ=255)                                                             aGCTGGGTGAGACACCGACTCATGAAGATATCATTGATAACAATCGATCCATCAACTGGCCTGTATAACGTGGCTGCCGGTGATTAAGAAAGCTGCACCTACCTAAGTAGTAGCAAACGCACACTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGT‑TCCAGGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTc                                                                                                                                                                   <  1:79495/251‑1 (MQ=255)                                                                tGGGTGAGACACCGACTCATGAAGATATCATTGCTAACAATCGATCCATCAACTGGCCTGTATAACGTGGCTGCCGGTGATTAAGAAAGCTGCACCTACCTAAGTAGTAGCAAACGCACACTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGT‑TCCAGGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCACCGt                                                                                                                                                                >  1:266974/1‑251 (MQ=255)                                                                       gaCACCGACTCATGAAGATATCATTGATAACAATCGATCCATCAACTGGCCTGTATAACGTGGCTGCCGGTGATTAAGAAAGCTGCACCTACCTAAGTAGTAGCAAACGCACACTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGT‑TCCAGGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTc                                                                                                                                                         <  1:467417/251‑1 (MQ=255)                                                                                tCATGAAGATATCATTGATAACAATCGATCCATCAACTGGCCTGTATAACGTGGCTGCCGGTGATTAAGAAAGCTGCACCTACCTAAGTAGTAGCAAACGCACACTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGT‑TCCAGGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGc                                                                                                                                                >  1:73837/1‑251 (MQ=255)                                                                                  aTGAAGCTATCAATGATAACAATCGATCCATCAACTGGCCTGTATAAAGTGGCTCCCCGAGCTTAAGAAAGCTGCACCTCCCTAAGTAGTAGCAAACGCACACTTTTTAGAAAAATCGATGGTCAGAAACTGGATAAGCAATTACGT‑TCCATGGTTGCTTTGGATTGACGTTGGATTCTGATCCTTGATCTATCCTCAAACGCCCAACCTCATTGGTAATGAACCACCTCCGTGAATGTCCGCTATGGcac                                                                                                                                              >  2:442880/1‑251 (MQ=255)                                                                                           tCATTGATAACAATCGATCCATCAACTGGCCTGTATAACGTGGCTGCCGGTGATTAAGAAAGCTGCACCTACCTAAGTAGTAGCAAACGCACACTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGT‑TCCAGGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGaa                                                                                                                                      >  1:111204/1‑250 (MQ=255)                                                                                                             ccATCAACTGGCCTGTATAACGTGGCTGCCGGTGATTAAGAAAGCTGCACCTACCTAAGTAGTAGCAAACGCACACTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGT‑TCCAGGGTTGCTTTTGATTTACGTTGGCGGCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTGTGATCTCCCCCCt                                                                                                                   <  2:443382/251‑1 (MQ=255)                                                                                                              cATCAACTGGCATGTATAACGTGGCTGCCGGTGATTAAGCAAGCTGCACCTACCTAAGTAGTAGCAAACGCACACTTTTTAGAAAAATCGATGGTCAGAAACTGGCTTAGCAATTCCGT‑TCCAGGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAACTTTTTTGATATCCCCCCTg                                                                                                                  >  2:27014/1‑251 (MQ=255)                                                                                                                   aCTGGCCTGTATAACGTGGCTGCCGGTGATTAAGAAAGCTGCACCTACCTAAGTAGTAGCAAACGCACACTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGT‑TCCAGGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATTAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATc                                                                                                             >  2:382939/1‑251 (MQ=255)                                                                                                                   aCTGGCCTGTATAACGTGGCTGCCGGTGATTAAGAAAGCTGCACCTACCTAAGTAGTAGCAAACGCACACTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGT‑TCCAGGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATc                                                                                                             >  1:290208/1‑251 (MQ=255)                                                                                                                                  gTGGCTGCCGGTGATTAAGAAAGCTGCACCTACCTAAGTAGTAGCAAACGCACACTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGT‑TCCAGGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCACCCCTGAAATCTAAACTTAGTCATGt                                                                                              >  1:301967/1‑251 (MQ=255)                                                                                                                                        gCCGGTGATTAAGAAAGCTGCACCTACCTAAGTAGTAGCAAACGCACACTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGT‑TCCAGGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTAAACGTAGTCATGTCACg                                                                                          <  2:394109/249‑1 (MQ=255)                                                                                                                                        gCCGGTGATTAAGAAAGCTGCACCTACCTAAGGTGTAGCAAACGCACACTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGT‑TCCAGGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCGCCTCCCTGAAATCTAAACTTAGTCATGTCACGtt                                                                                        <  2:497105/251‑1 (MQ=255)                                                                                                                                         ccGGTGATTAAGAAAGCTGCTCCTACCTAAGTAGTAGCAAACGCACCCTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGT‑TCCAGGGTTTCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCGCCGTGAATGTCCGCTCTGGCACAGATCGAAATTTTTTGATCTCCCCCCTTAAATCTAAACTTAGTCATGTCACGttt                                                                                       <  1:78704/251‑1 (MQ=255)                                                                                                                                                    gAAAGCTGCACCTACCTAAGTAGTAGCAAACGCACACTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGT‑TCCAGGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGATCCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTAAACTTAGTCATGTCACGTTGTTGGGTTTCTa                                                                            <  2:73837/251‑1 (MQ=255)                                                                                                                                                     aaaGCTGCACCTACCTAAGTAGTAGCAAACGCACACTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGT‑TCCAGGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTAAACTTAGTCATGTCACGTTTTTGGGTTTCTa                                                                            >  2:64092/1‑250 (MQ=255)                                                                                                                                                                    taAGTAGTAACAAACGCACACTTTTTAGACAAATCGATGGTAAGAAACTGGATTAGCACTTACGT‑TCCAGGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCAAAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTACACTTAGTCATGTCACGTTTTTGGGTTTCTAAAATTTTAACTTcgcg                                                            >  2:481524/1‑251 (MQ=255)                                                                                                                                                                               aaaCGCACACTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGT‑TCCAGGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTAAACTTAGTCATGTCACGTTTTTGGGTTTCTAAAATTTTAACTTCGCGTTTTTCGTTGc                                                 >  2:259709/1‑251 (MQ=255)                                                                                                                                                                                       acTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGT‑TCCAGGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTAAACTTAGTCATGTCACGTTTTTGGGTTTCTAAAATTTTAACTTCGCGTTTTTCGTTGCCGTAAggg                                         <  2:127978/251‑1 (MQ=255)                                                                                                                                                                                       acTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGT‑TCCAGGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTAAACTTAGTCATGTCACGTTTTTGGGTTTCTAAAATTTTAACTTCGCGTTTTTCGTTGCCGTAAggg                                         <  1:23205/251‑1 (MQ=255)                                                                                                                                                                                                   aaTCGATGGTCAGAAACTGGACTAGCAATTCCGTATCC‑GGGTTGCTTTTGATTTCCGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCATCTCGGTGAATGTCAGCTCTGGCATAGATCGAAATTTTTTAATCTCCCACCTCTAATCTAAACTTAGTCATGTCACGTTTTTTGGTTTCTAAAATTTTAATTTCGCGCTTTTCTTTGCCGTCAGGGTTATACAGAAAt                             >  2:390135/1‑251 (MQ=255)                                                                                                                                                                                                      cGATGGTCAGCACCTGGATTAGCAATTCCGT‑TCCAGGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAACTCTAAACTTAGTCATGTCACGTTTTTGGGTTTCTCAAATTTTAACTTCGCGTTTTTCGTTGCCGTAAGGGTTATACAGAAATGTc                          >  2:405618/1‑251 (MQ=255)                                                                                                                                                                                                              aGAAACTGGATTAGCAATTCCGT‑TCCAGGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTAAACTTAGTCATGTCACGTTTTTGGGTTTCTAAAATTTTAACTTCGCGTTTTTCGTTGCCGTAAGGGTTATACAGAAATGTCCGTTAAGc                  >  2:345990/1‑251 (MQ=255)                                                                                                                                                                                                                           gCAATTCCGT‑TCCAGGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACATAGCGAAATTTTTTGATCTCCCCCCTGTAATCGAATCTTAGTCATGTCACGTTTTTGGGTTTCTAAAAGTTTAACTTCGCGTTTTGCGTTGCCGTAAGGGTTATACAGAAATGTCCGTTAAGCAGAGTTCAAAatt     <  1:284418/251‑1 (MQ=255)                                                                                                                                                                                                                              aTTCCGT‑TCCAGGGTTGATTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTAAACTTAGTCATGTCACGTTGTTGGGTTTCTAAAATTTTAACTTCGCGTTTTTCGTTGCCGTAAGGGTTATACAGAAATGTCCGTTAAGCAGAGTTCAAAattgat  <  2:369448/251‑1 (MQ=255)                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                CTCTGGCGAAAAAGGGAGAAGGACGGGCCCGTCTGCCTTCACTTTATGCCAGCTTCGGTAAGCTGGGTGAGACACCGACTCATGAAGATATCATTGATAACAATCGATCCATCAACTGGCCTGTATAACGTGGCTGCCGGTGATTAAGAAAGCTGCACCTACCTAAGTAGTAGCAAACGCACACTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGT‑TCCATGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTAAACTTAGTCATGTCACGTTTTTGGGTTTCTAAAATTTTAACTTCGCGTTTTTCGTTGCCGTAAGGGTTATACAGAAATGTCCGTTAAGCAGAGTTCAAAATTGAT  >  NC_000913/4510006‑4510477

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑