Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	366,409	C→T	intergenic (‑104/+19)	lacZ ← / ← lacI	beta‑galactosidase/DNA‑binding transcriptional repressor LacI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	366,409	0	C	T	100.0%	103.1 / NA	28	intergenic (‑104/+19)	lacZ/lacI	beta‑galactosidase/DNA‑binding transcriptional repressor LacI
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (16/12);  total (16/12)

GGCGATCGGTGCGGGCCTCTTCGCTATTACGCCAGCTGGCGAAAGGGGGATGTGCTGCAAGGCGATTAAGTTGGGTAACGCCAGGGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTGAATCCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCG‑TATTGGGCG‑CC‑AGGGTGG‑TTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCC‑TTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTT‑GCCCCAGCAGGCGAAAATCC  >  NC_000913/366164‑366638                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                            ggCGATCGGTGCGGGCCTCTTCGCTATTACGCCAGCTGGCGAAAGGGGGATGTGCTGCAAGGCGATTAAGTTGGGTAACGCCAGGGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTGAATCCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTTACAtt                                                                                                                                                                                                                                        <  1:162581/251‑1 (MQ=255)   cGATCGGTGCGGGCCTCTTCGCTATTACGCCAGCTGGCGAAAGGGGGATGTGCTGCAAGGCGATTAAGTTGGGTAACGCCAGGGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTGAATCCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTTACATTaa                                                                                                                                                                                                                                      <  2:72957/251‑1 (MQ=255)                                 cAGCTGGCGAAAGGGGGATGTGCTGCAAGGCGATTAAGTTGGGTAACGCCAGGGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTGAATCCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTTACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGt                                                                                                                                                                                                        >  2:451782/1‑251 (MQ=255)                                  aGCTGGCGAAAGGGGGATGTGCTGCAAGGCGATTAAGTTGGGTAACGCCAGGGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTGAATCCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTTACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTc                                                                                                                                                                                                       >  2:121723/1‑251 (MQ=255)                                  aGCTGGCGAAAGGGGGATGTGCTGCAAGGCGATTAAGTTGGGTAACACCAGGGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTGAATCCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGACGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTTACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTc                                                                                                                                                                                                       >  1:256467/1‑251 (MQ=255)                                      ggCGAAAGGGGGATGTGCTGCAAGGCGATTAAGTTGGGTAACGCCAGGGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTGAATCCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTTACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCggg                                                                                                                                                                                                    >  2:161653/1‑250 (MQ=255)                                                 gATGTGCTGCAAGGCGATTAAGTTGGGTAACGCCTGGGGTTTCCCATGCACGACGTTGTAAAACGACGGCCAGTGAATCCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATGCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGTGGTGCCTAATGAGTGAGCTAACTTACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCATTCGGGAAACCTGTCGTg                                                                                                                                                                                        <  2:166058/251‑1 (MQ=255)                                                        tgcAAGGCGATTAAGTTGGGTAACGCCAGGGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTGAATCCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACACCATACGAGCCGGAAGCATAAAGCGGAAAGCCTGGGGAGCCTAATGAGTAAGCTAACTTACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCCGCTg                                                                                                                                                                                 >  1:495564/1‑251 (MQ=255)                                                           aaGGCGATTAAGTTGGGTAACGCCAGGGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTGAATCCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTTACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCAt                                                                                                                                                                              >  1:369699/1‑251 (MQ=255)                                                              gCGATTAAGTTGGGTAACGCCAGGGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTGAATCCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTTACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTa                                                                                                                                                                            >  1:130536/1‑250 (MQ=255)                                                                gATTAAGTTGGGTAACGCCAGGGTTTTCCCAGTCACGCAGTAGTAAAACGACGGCCAGTGAATCCGTAATCATGGTCATAGCTGTTTCCTGTGTGAACTTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAACGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTTACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTACCAGCTGCATTAATg                                                                                                                                                                         >  2:458369/1‑251 (MQ=255)                                                                         gggTAACGCCAGGGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTGAATCCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTAACCGCTCCCAATGCCACACAACATCCGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACGTACATCAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCa                                                                                                                                                                <  2:440794/251‑1 (MQ=255)                                                                                  cAGGGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTGAATCCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTTACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCggg                                                                                                                                                       >  1:149495/1‑251 (MQ=255)                                                                                          tCCCAGTCACGACGTTGTAAAACGACGGCCAGTGAATCCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTTACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGt                                                                                                                                                                                         >  2:185148/1‑209 (MQ=255)                                                                                          tCCCAGTCACGACGTTGTAAAACGACGGCCAGTGAATCCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTTACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGt                                                                                                                                                                                         <  1:185148/209‑1 (MQ=255)                                                                                                          gTAAAACGACGGCCAGTGAATCCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTTACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCG‑TATTGGgcg                                                                                                                              <  1:324107/251‑1 (MQ=255)                                                                                                                 gacgGCCAGTGAATCCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTTACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCG‑TATTGGGCG‑CC‑AGggt                                                                                                                     <  1:464372/251‑1 (MQ=255)                                                                                                                             aTCCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTTACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCG‑TATTGGGCG‑CC‑AGGGTGG‑Tttttctttt                                                                                                        <  1:474451/251‑1 (MQ=255)                                                                                                                                       aTGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTTACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCG‑TATTGGGCG‑CC‑AGGGTGG‑TTTTTCTTTTCACCAGTgag                                                                                              <  1:179560/251‑1 (MQ=255)                                                                                                                                         ggTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTTACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCG‑TATTGGGCG‑CC‑AGGGTGG‑TTTTTCTTTTCACCAGTgaga                                                                                             <  1:161653/250‑1 (MQ=255)                                                                                                                                                                                      cacacaACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTTACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCG‑TATTTGGGG‑CC‑CGGGGGG‑GTTTTCTTTTTACCCGTGGGAGGGGGAACAGCTGGTTGCCC‑TTCACCCCCTGGCCCCGAGAGAGTTg                                              >  1:5609/1‑251 (MQ=255)                                                                                                                                                                                         acaacaTACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGGGCCTAATGAGTGAGCTAACTTACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCG‑TATTGGGCG‑CC‑AGGGTGG‑TTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCC‑TTCACCGCCTGGCCCTGAGAGAGTTgcag                                           <  2:369699/251‑1 (MQ=255)                                                                                                                                                                                                  gAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTTACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGGTTTCG‑GATTTGGCG‑CCCGGGGGGG‑TTTTTTTTTTC‑CCCGGGCGACGGGCAACAGCTGATTGCCC‑TTCTCCCCCTCGCCCTCAGAGAGTTGCCGCAAGCgggc                                  >  1:118734/1‑249 (MQ=255)                                                                                                                                                                                                     ccGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTTACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCG‑TATTGGGCG‑CC‑AGGGTGG‑TTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCC‑TTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCAc                               <  1:86883/251‑1 (MQ=255)                                                                                                                                                                                                              tAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTTACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGGTTTTGGG‑G‑GC‑AGGGGGG‑GTTTTTTTTTTACCAGTGCGACGGGCGAAAGCCGATTGTCC‑TTCCCCCCCCGGCCCTTCGAGGGGTGCAGCAAGCGGGCCACGcgggggtt                      >  2:376894/1‑244 (MQ=255)                                                                                                                                                                                                                       aaGCCTGGGGTGCCTAATGAGTGAGCTAACTTACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGACAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTTTC‑TATTGGGGG‑CC‑AGGGGGGGTTTTTTTTTTAACC‑GAGGGACGGGCAAAAGCTGGTTGCCC‑TTCAACGCCAGGCCCTGAGGGGGTTTCAGAAAGGGGTGCACGcggggtttcccccgc               >  1:462479/1‑235 (MQ=255)                                                                                                                                                                                                                        aGCCTGGGGTGCCTAATGAGTGAGCTAACTTACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCG‑TCTTTGGCG‑GC‑CGGGGGG‑TTTTTTTTTTCACCAGTGTGACGGGCAACAGCCGCTTGCCCCTTC‑CCCCCTGACCCTGAGAGAGGTGGAGCGAGCGGTTCCCGCTGGGTT‑TCCCcaaccgg           >  1:74393/1‑246 (MQ=255)                                                                                                                                                                                                                                  gCCTAATGAGTGAGCTAACTTACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTTCG‑TCTTCGTCGGCC‑GGGGGGG‑TTTTT‑TTTTTACCCGGGGGACCGGCAACAGCCGCATGCCC‑TTTACCGCCCGGCCCTGTGAGAGGTTCAGCAAGCGGGCCCCCCTGGTTTTGccccgccgggggaaatccc  >  1:451289/1‑236 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                            GGCGATCGGTGCGGGCCTCTTCGCTATTACGCCAGCTGGCGAAAGGGGGATGTGCTGCAAGGCGATTAAGTTGGGTAACGCCAGGGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTGAATCCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCG‑TATTGGGCG‑CC‑AGGGTGG‑TTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCC‑TTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTT‑GCCCCAGCAGGCGAAAATCC  >  NC_000913/366164‑366638

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑