Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,287,464	T→A	intergenic (‑93/‑147)	acs ← / → ytcB	acetyl‑CoA synthetase (AMP‑forming)/protein YtcB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,287,464	0	T	A	100.0%	149.1 / NA	41	intergenic (‑93/‑147)	acs/ytcB	acetyl‑CoA synthetase (AMP‑forming)/protein YtcB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (18/23);  total (18/23)

GGTTTGATCCAGTCAAGAATTTTTCCCTGTTCGCCCCAGAAGGTATCAGGTACGTTAATAGATTGTTGATACATCGCCTCGTACTGCTGAGGGTTTATCAGGCAACGGTCTGCGATGTTGGCAGGAATGGTGTGTTTGTGAATTTGGCTCATGCTTTTGTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAATGTAGGGGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGATCACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAATTCTTTGAGGAACATGCAGTTATGCATGCTGTTGAAAAAGAGGAAGATACTGACTAACTCTAAAGTGGTATTTTACATGCACTTACAATTGATTAAAGACAACATTCACAGTGTGGTTATTTGTTACACATAGGGGCGAGCAATGTCATGACAGTGTAGGTGCGGTTACTGTCGTG  >  NC_000913/4287220‑4287710                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                       ggTTTGATCCAGTCAAGAATTTTTCCCTGTTCGCCCCAGAAGGTATCAGGTACGTTAATAGATTGTTGATACATCGCCTCGTACTGCTGAGGGTTTATCAGGCAACGGTCTGCGATGTTGGCAGGAATGGTGTGTTTGTGAATTTGGCTCATGCTTTTGTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAATGTAGGGGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGAACACGCa                                                                                                                                                                                                                                                  <  1:74244/251‑1 (MQ=255)               aaGAATTTTTCCCTGTTCGCCCCAGAAGGTATCAGGTACGTTAATAGATTGTTGATACATCGCCTCGTACTGCTGAGGGTTTATCAGGCAACGGTCTGCGATGTTGGCAGGAATGGTGTGTTTGTGAATTTGGCTCATGCTTTTGTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAATGTAGGGGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGAACACGCAAAAGTAGAATtgtg                                                                                                                                                                                                                                    >  1:430859/1‑251 (MQ=255)                        tCCCTGTTCGCCCCAGAAGGTATCAGGTACGTTAATAGATTGTTGATACATCGCCTCGTACTGCTGAGGGTTTATCAGGCAACGGTCTGCGATGTTGGCAGGAATGGTGTGTTTGTGAATTTGGCTCATGCTTTTGTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAATGTAGGGGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGAACACGCAAAAGTAGAATTGTGCAAATAAAc                                                                                                                                                                                                                           >  1:355248/1‑251 (MQ=255)                           cTGTTCGCCCCAGAAGGTATCAGGTACGTTAATAGATTGTTGATACATCGCCTCGTACTGCTGAGGGTTTATCAGGCCCCGGTCTGCGATGTTGGCAGGAATGGTGTGTTTGTGACTTTGGCTCATGCTTTTGTTCTCCTTGTAGGATGTTAATACTATGTGGCATAAGCGTTAAATGTAGGGGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGAACACGAAAAAGTAGAATTGTGCAAATAAACGGc                                                                                                                                                                                                                        >  1:68155/1‑251 (MQ=255)                                cGCCCCAGAAGGTATCAGGTACGTTAATAGATTGTTGATACATCGCCTCGTTCTGCTGAGGGTTTATCAGGCAACGGTCTGCGATGTTGGCAGGAAGGGTGTGTTTGTGAATTTGGCTCATGCGTTTGTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAATGTAGGGGTATTGGCAGTTTTGTTTAGTAGTTTGGCGACAGAACACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGt                                                                                                                                                                                                                   <  1:192027/251‑1 (MQ=255)                                     cAGAAGGTATCAGGGACGTTAAGAGATGGTTGATCCATCGCCTCGTACTGCTGAGGGTTTAGCAGGCAACTGACTTCGATGTTTGCAGGAATGGTGTGTTTGTGAATTTGGCTCATGCTTTTGTTCTCCTTGTATGATGTTAATAATATGGTGCATAATCGTTAAATGTAGGGGTATTGGCATTTTTGTTTAGTATTTGGGCGACAGAACACGCAAAAGTAGAATTGTGCAAATAAACCGCAGTGTAAttt                                                                                                                                                                                                              <  2:33061/251‑1 (MQ=255)                                                 gggaCGTTAATAGATTGTTGATACATCGCCTCGTACTGCTGAGGGTTTATCAGGCAACGGTCTGCGATGTTGGCAGGAATGGTGTGTTTGTGAATTTGGCTCATGCTTTTTTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAATGTAGGGGTATTGGCAGTGTTGTTTAGTATTTGGGCGACAGAACACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAAGGTTTGAATGTCAGc                                                                                                                                                                                                  <  2:230585/248‑1 (MQ=255)                                                         aagaGATTGTTGATACATCGCCTCGTTCTGCTGAGGGTTTATCAGGCAACGGTCTGCGATGTTGGCAGGAATGGTGTGTTTGTGAATTTGGCTCATGCTTTTGTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAATGTAGGGGTATTGGCAGTTTTTTTTAGGATTTGGGCGACAGAACACGCAAAAGCAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCATCAACAAAAg                                                                                                                                                                                          <  2:483105/248‑1 (MQ=255)                                                                    gATACATCGCCTCGTACTGCTGAGGGTTTATCAGGCAACGGTCTGCGATGTTGGCAGGAATGGTGTGTTTGTGAATTTGGCTCATGCTTTTGTTATCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAATGTCGGGGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGAACACGCAAAAGTAGAATTGCGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAAttt                                                                                                                                                                               >  1:466749/1‑250 (MQ=255)                                                                              cTCGTACTGCTGAGGGTTTATCAGGCAACGGTCTGCGATGTTGTCAGGAATGGTGTGTTTGTGAATTTGGCGCATGCTTTTGTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAATGTAGGGGTAGTGGCAGTTTTGTTTAGTATTTGGGCGACAGAACACGCAAAAGTAGAATTGTGCaa                                                                                                                                                                                                                                 <  2:73552/191‑1 (MQ=255)                                                                              cTCGTACTGCTGAGGGTTTATCAGGCAACGGTCTGCGATGTTGGCAGGAATGGTGTGTTTGTGAATTTGGCTCATGCTTTTGTTCTACTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAATGTAGGGGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGAACACGCAAAAGTAGAATTGTGCaa                                                                                                                                                                                                                                 >  1:73552/1‑191 (MQ=255)                                                                                   aCTGCTGAGGGTTTATCAGGCAACGGTCTGCGATGTTGGCAGGAATGGTGTGTTTGTGAATTTGGCTCATGCTTTTGTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAATGTAGGGGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGAACACGCAAAAGTAGAATtgtg                                                                                                                                                                                                                                    >  1:218986/1‑183 (MQ=255)                                                                                   aCTGCTGAGGGTTTATCAGGCAACGGTCTGCGATGTTGGCAGGAATGGTGTGTTTGTGAATTTGGCTCATGCTTTTGTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAATGTAGGGGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGAACACGCAAAAGTAGAATtgtg                                                                                                                                                                                                                                    <  2:218986/183‑1 (MQ=255)                                                                                   aCTGCTGAGGGTTTATCAGGCAACGGTATGCGATGTTGGCAGGAATGGTGTGTTTGTGAATTTGGCGCATGCTTTTGTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAATGTAGGGGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGAACACGAAACAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAATTCTTTGAGGAACATga                                                                                                                                                                 >  1:479123/1‑249 (MQ=255)                                                                                         gAGGGTTTATCAGGCAACGGTCTGCGATGTTGGCAGGAATGGTGTGTTTGTGAATTTGGCTCATGCTTTTGTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAATGTAGGGGTATTGGCAGTTTTGTTTAGTAGTTGGGCGACAGAACACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTTAAGGTCAGCAACAAAAGTTGATTAATTCTTTGAGGAACATGCAGTTat                                                                                                                                                           <  2:428914/250‑1 (MQ=255)                                                                                             gTTTATCAGGCAACGGTCTGCGATGTTGGCAGGAATGGTGTGTTTGTGAATTTGGCTCATGCTTTTGTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAATGTAGGGGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGAACACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAATTCTTTGAGGAACATGCAGTTatgcatg                                                                                                                                                      <  1:344174/251‑1 (MQ=255)                                                                                                                gCGATGTTGGCAGGAATGGTGTTTTTGTGAATTTGGCTCATGCTTTTGTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAATGTAGGGGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGAACACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAATTCTTTGAGGAACATGCAGTTATGCATGCTGTTGAAAAAGAGGAAg                                                                                                                                    <  2:40632/250‑1 (MQ=255)                                                                                                                             gAATGGTGTGTTTGTGAATTTGGCTCATGCTTTTGTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAATGTAGGGGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGAACACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAATTCTTTGAGGAACATGCAGTTATGCATGCTGTTGAAAAAGAGGAAGATACTGACTAActc                                                                                                                      >  1:319056/1‑251 (MQ=255)                                                                                                                                              aTTTGGCTCATGCTGTTGTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAATGTAGGGGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGAACACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAATTCTTTGAGGAACATGCAGTTATGCATGCTGTTGAAAAAGAGGAAGATACTGACTAACTCTAAAGTGGTATTTTACa                                                                                                     <  2:355248/251‑1 (MQ=255)                                                                                                                                                       aTGCTTTTGTTCTCCGTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAATGTAGGGGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGAACACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAATTCTTTGAGGAACATGCAGTTATGCATGCTGTTGAAAAAGAGGAAGAGACTGACTAACTCTAAAGTGGTATTTTACATGCACTTAc                                                                                            <  2:212622/251‑1 (MQ=255)                                                                                                                                                           ttttGTGCTCCTTGTAGGATGTTAATAATATGTGCCATAAGCTTTATCTGTAGGGGTAGTGGCAGTTTTTTTTAGTATTTGGGAGAAAGAACACGCAAGAGTAGAATTGTGCAAATAAAAGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGATGATTAATTCTTTGTGGAACATGCAGTTATGCATGATGATGGAAAAGAGGAATATACTGACTAACTCTAAGGTGGGATTTTACATGCACTTACactt                                                                                        >  2:239259/1‑248 (MQ=255)                                                                                                                                                                                 taataaTATGTGGCATAAGCGTTAAATGTAGGGGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGAACACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGATGATTAATTCTTTGAGGAACATGCAGTTATGCATGCTGTTGAAAAAGAGGACGATAATGACTAACTCTAAAGTGGTATTTTACATGCACTTACCATTGATTAAAGACAACATTCACAgt                                                                  >  1:256840/1‑251 (MQ=255)                                                                                                                                                                                     aataTGTGGCATAAGCGTTAAATGTAGGGGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGAACACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAATTCTTTGAGGAACATGCAGTTATGCATGCTGTTGAAAAAGAGGAAGATACTGACTAACTCTAAAGTGGTATTTTACATGCACTTACAATTGATTAAAGACAACATTCACAGTGTgg                                                              <  1:159266/251‑1 (MQ=255)                                                                                                                                                                                            ggCATAAGCGGTAAATGTAGGGGTATTGGCAGTTTTGTTTAGTATTAGGGCTGCAGAACACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTGTTGAAGGTCAGCAAAATAAGTTGATTAATTCTTTGAGGAACATGCAGCGATGCATGCTGTTGAAAAAGAGGAAGATACTGACTAACTCGAAAGTGGGAGTGTACATGCTCTTACAATTGATTAAAGACAACATTCACAGTGTGGTTAttt                                                        <  2:420069/250‑1 (MQ=255)                                                                                                                                                                                              cATAAGCGTTAAATGTAGGGGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGAACACGCTAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAATTCTTTGAGGAACATGCAGTTATGCATGCTGTTGAAAAAGAGGAAGATACTGACTAACTCTAAAGTGGTATTTTACATGCACTTACAATTGATTAAAGACAACATTCACAGTGTGGTTATTTGtt                                                     >  1:213737/1‑251 (MQ=255)                                                                                                                                                                                                  aGCGTTAAATGTAGGGGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGAACACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAATTCTTTGAGGAACATGCAGTTATGCATGCTGTTGAAAAAGAGGAAGATACTGACTAACTCTAAAGTGGTATTTTACATGCACTTACAATTGATTAAAGACAACATTCACAGTGTGGTTATTTGTTacac                                                 <  2:360318/251‑1 (MQ=255)                                                                                                                                                                                                  aGCGTTAAATGTAGGGGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGAACACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAATTCTTTGAGGAACATGCAGTTATGCATGCTGTTGAAAAAGAGGAAGATACTGACTAACTCTAAAGTGGTATTTTACATGCACTTACAATTGATTAAAGACAACATTCACAGTGTGGTTATTTGTTa                                                    >  2:340335/1‑248 (MQ=255)                                                                                                                                                                                                  aGCGTTAAATGTAGGGGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGAACACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAATTCTTTGAGGAACATGCAGTTATGCATGCTGTTGAAAAAGAGGAAGATACTGACTAACTCTAAAGTGGTATTTTACATGCACTTACAATTGATTAAAGACAACATTCACAGTGTGGTTATTTGTTa                                                    <  1:340335/248‑1 (MQ=255)                                                                                                                                                                                                       tAAATGTAGGGGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGAACACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAATTCTTTGAGGAACATGCAGTTATGCATGCTGTTGAAAAAGAGGAAGATACTGACTAACTCTAAAGTGGTATTTTACATGCACTTACAATTGATTAAAGACAACATTCACAGTGTGGTTATTTGTTACACATAgg                                            >  1:280681/1‑251 (MQ=255)                                                                                                                                                                                                              aGGGGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGAACACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAATTCTTTGAGGAACATGCAGTTATGCATGCTGTTGAAAAAGATGAAGATACTGACTAACTCTAAAGTGGTATTTTACATGCGCTTACAATTGATTAAAGACAACATTCACAGTGTGGTTATTTGTTACACATAGGGGCGAGc                                     <  2:489884/251‑1 (MQ=255)                                                                                                                                                                                                              aGGGGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGAACACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAATTCTTTGAGGAACATGCAGTTATGCATGCTGTTGAAAAAGAGGAAGATACTGACTAACTCTAAAGTGGTATTTTACATGCACTTACAATTGATTAAAGACAACATTCACAGTGTGGTTATTTGTTACACATAGGGGCGAg                                      <  1:131468/250‑1 (MQ=255)                                                                                                                                                                                                              aGGGGTATTGGCAGTTTTGATTAGTATTTGGGCGACAGAACACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAATTCTTTGAGGAACATGCAGTTATGCATGCTGTTGAAAAAGAGGAAGATACTGACTAACTCTAAAGTGGTATTTTACATGCACTTACAATTGATTAAAGACAACATTCACAGTGTGGTTATTTGTTACACATAGGGGAGAGc                                     >  2:3285/1‑251 (MQ=255)                                                                                                                                                                                                                     ttGGCAGTTTTGTTTAGTATTTGGGCGACAGAACACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAATTCTTTGAGGAACATGCAGTTATGCATGCTGTTGAAAAAGAGGAAGATACTGACTAACTCTAAAGTGGTATTTTACATGCACTTACAATTGATTAAAGACAACATTCACAGTGTGGTTATTTGTTACACATAGGGGCGAGCAATGTc                               >  2:135213/1‑250 (MQ=255)                                                                                                                                                                                                                      tGGCAGTTTTGTTTAGTATTTGGGCGACAGAACACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAATTCTTTGAGGAACATGCAGTTATGCATGCTGTTGAAAAAGAGGAAGATACTGACTAACTCTAAAGTGGTATTTTACATGCACTTACAATTGATTAAAGACAACATTCACAGTGTGGTTATTTGTTACACATAGGGGCGAGCAATGTCAt                             >  1:188729/1‑251 (MQ=255)                                                                                                                                                                                                                         cAGTTTTGTTTAGTATTTGGGCGACAGAACACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAATTCTTTGAGGAACATGCAGTTATGCATGCTGTTGAAAAAGAGGAAGATACTGACTAACTCTAAAGTGGTATTTTACATGCACTTACAATTGATTAAAGACAACATTCACAGTGTGGTTATTTGTTACACATAGGGGCGAGCAATGTCATGAc                          <  1:135213/251‑1 (MQ=255)                                                                                                                                                                                                                          aGTTTTGTTTAGTATTTGGGCGACAGAACACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAATTCTTTGAGGAACATGCAGTTATGCATGCTGTTGAAAAAGAGGAAGATACTGACTAACTCTAAAGTGGTATTTTACATGCACTTACAATTGATTAAAGACAACATTCACAGTGTGGTTATTTGTTACACATAGGGGCGAGCAATGTCATGAc                          >  1:277441/1‑250 (MQ=255)                                                                                                                                                                                                                                    aGTATTTGGGCGACAGAACACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAATTCTTTGAGGAACATGCAGTTATGCATGCTGTTGAAAAAGAGGAAGATACTGACTAACTCTAAAGTGGTATTTTACATGCACTTACAATTGATTAAAGACAACATTCACAGTGTGGTTATTTGTTACACATAGGGGCGAGCAATGTCATGACAGTGTAGGTg                <  1:108084/250‑1 (MQ=255)                                                                                                                                                                                                                                          tGGGCGACAGAACACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAATTCTTTGAGGAACATGCAGTTATGCATGCTGTTGAAAAAGAGGAAGATACTGACTAACTCTAAAGTGGTATTTTACATGCACTTACAATTGATTAAAGACAACATTCACAGTGTGGTTATTTGTTACACATAGGGGCGAGCAATGTCATGACAGTGTAGGTGCGGTTa          <  2:319056/250‑1 (MQ=255)                                                                                                                                                                                                                                          tGGGCGACAGAACACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAATTCTTTGAGGAACATGCAGTTATGCATGCTGTTGAAAAAGAGGAAGATACTGACTAACTCTAAAGTGGTATTTTACATGCACTTACAATTGATTAAAGACAACATTCACAGTGTGGTTATTTGGTACACATAGGGGCGAGCAATGTCATGACAGTGTAGGTGCGGTTa          <  1:51210/250‑1 (MQ=255)                                                                                                                                                                                                                                            ggCGACAGAACACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAATTCTTTGAGGAACATGCAGTTATGCATGCTGTTGAAAAAGAGGAAGATACTGACTAACGCGAAAGTGGTATTTTACATGCACTTACAATTGATGAAAGACAACATTCACAGTGTGGTTAGTTGTTACACATATGGGCGAGCAATAGCAGGACAGTGTAGGTGCGGTTACTg       <  2:334375/251‑1 (MQ=255)                                                                                                                                                                                                                                                 cAGAACACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAATTCTTTGAGGAACATGCAGTTATGCATGCTGTTGAAAAAGAGGAAGATACTGACTAACTCTAAAGTGGTATTTTACATGCACTTACAATTGATTAAAGACAACATTCACAGTGTGGTTATTTGTTACACATAGGGGCGAGCAATGTCATGACAGTGTAGGTGCGGTTACTGTCGTg  >  2:140214/1‑251 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                       GGTTTGATCCAGTCAAGAATTTTTCCCTGTTCGCCCCAGAAGGTATCAGGTACGTTAATAGATTGTTGATACATCGCCTCGTACTGCTGAGGGTTTATCAGGCAACGGTCTGCGATGTTGGCAGGAATGGTGTGTTTGTGAATTTGGCTCATGCTTTTGTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAATGTAGGGGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGATCACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAATTCTTTGAGGAACATGCAGTTATGCATGCTGTTGAAAAAGAGGAAGATACTGACTAACTCTAAAGTGGTATTTTACATGCACTTACAATTGATTAAAGACAACATTCACAGTGTGGTTATTTGTTACACATAGGGGCGAGCAATGTCATGACAGTGTAGGTGCGGTTACTGTCGTG  >  NC_000913/4287220‑4287710

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑