Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,553,662	A→C	Q193P (CAG→CCG)	malT →	DNA‑binding transcriptional activator MalT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,553,662	0	A	C	100.0%	212.3 / NA	63	Q193P (CAG→CCG)	malT	DNA‑binding transcriptional activator MalT
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (35/28);  total (35/28)

CAGCTTTTCATTGAGCTGGCGGAATGGCATAGCCCACTTTATCTGGTCATCGATGACTATCATCTGATCACTAATCCAGTGATCCACGAGTCAATGCGCTTCTTTATTCGCCATCAACCAGAAAATCTCACCCTGGTGGTGTTGT‑CACGCAACCTTCCGCAACTGGGCATTGCCAATCTGCGTGTTCGTGATCAACTGCTGGAAATTGGCAGTCAGCAACTGGCATTTACCCATCAGGAAGCGAAGCAGTTTTTT‑GATTGCCGTCTGTCATCGCCGATTGAAGCCGCAGAAAGCAGTCGGATTTGCGATGACGTTTCCGGTTGGGCGACGGCACTACAGCTAATCGCCCTCTCCGCCCGGCAGAATACCCACTCAGCCCATAAGTCGGCACGCCGCCTGGCGGGAATCAATGCCAGCCATCTTTCGGATTATCTGGTCGATGAGGTTTTGGATAACGTCGATCTCGCAACGCGCCATTTTCTGTTG  >  NC_000913/3553415‑3553900                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                cAGCTTTTCATTGAGCTGGCGGAATGGCATAGCCCACTTTATCTGGTCATCGATGACTATCATCTGATCACTAATCCAGTGATCCACGAGTCAATGCGCTTCTTTATTCGCCATCAACCAGAAAATCTCACCCTGGTGGTGTTGT‑CACGCAACCTTCCGCAACTGGGCATTGCCAATCTGCGTGTTCGTGATCAACTGCTGGAAATTGGCAGTCAGCAACTGGCATTTACCCATCAGGAAGCGAAGCCGtt                                                                                                                                                                                                                                              <  2:228040/251‑1 (MQ=255)      tttCATTGAGCTGGCGGAATGGCATAGCCCACTTTATCTGGTCATCGATGACTATCATCTGATCACTAATCCAGTGATCCACGAGTCAATGCGCTTCTTTATTCGCCATCAACCAGAAAATCTCACCCTGGTGGTGTTGT‑CACGCAACCTTCCGCAACTGGGCATTGCCAATCTGCGTGTTCGTGATCAACTGCTGGAAATTGGCAGTCAGCAACTGGCATTTACCCATCAGGAAGCGAAGCCGttttt                                                                                                                                                                                                                                           <  1:177626/249‑1 (MQ=255)        tCATTGAGCTGGCGGCATGGCATAGCCCACTTTATCTGGTCATCGATGACTATCATCTGATCACTAATCCAGTGATCCACGAGTCAATGCGCTTCTTTATTCGCCATCAACCAGAAAATCTCACCCTGGTGGTGTTGT‑CACGCAACCTTCCGCAACTGGGCATTGCCAATCTGCGTGTTCGTGATCAACTGCTGGAAATTGGCAGTCAGCAACTGGCATTTACCCATCAGGAAGCGAAGCCGTTTTTT‑GAt                                                                                                                                                                                                                                      >  2:75389/1‑251 (MQ=255)        tCATTGAGCTGGCGGAATGGCATAGCCCACTTTATCTGGTCATCGATGACTATCATCTGATCACTAATCCAGTGATCCACGAGTCAATGCGCTTCTTTATTCGCCATCAACCAGAAAATCTCACCCTGGTGGTGTTGT‑CACGCAACCTTCCGCAACTGGGCATTGCCAATCTGCGTGTTCGTGATCAACTGCTGGAAATTGGCAGTCAGCAACTGGCATTTACCCATCAGGAAGCGAAGCCGTTTTTT‑GAt                                                                                                                                                                                                                                      >  1:292312/1‑251 (MQ=255)             gAGCTGGCGGAATGGCATAGCCCACTTTATCTGGTCATCGATGACTATCATCTGATCACTAATCCAGTGATCCACGAGTCAATGCGCTTCTTTATTCGCCATCAACCAGAAAATCTCACCCTGGTGGTGTTGT‑CACGCAACCTTCCGCAACTGGGCATTGCCAATCTGCGTGTTCGTGATCAACTGCTGGAAATTGGCAGTCAGCAACTGGCATTTACCCATCAGGAAGCGAAGCCGTTTTTT‑GATTGCCg                                                                                                                                                                                                                                 <  1:498180/251‑1 (MQ=255)                   gCGGAATGGCATAGCCCACTTTATCTGGTCATCGATGACTATCATCTGATCACTAATCCAGTGATCCACGAGTCAATGCGCTTCTTTATTCGCCATCAACCAGAAAATCTCACCCTGGTGGTGTTGT‑CACGCAACCTTCCGCAACTGGGCATTGCCAATCTGCGTGTTCGTGATCAACTGCTGGAAATTGGCAGTCAGCAACTGGCATTTACCCATCAGGAAGCGAAGCCGTTTTTT‑GATTGCCGTCTGTc                                                                                                                                                                                                                           <  1:199776/251‑1 (MQ=255)                    cGGAATGGCACAGCCCACTTTATCTGGTCATCGATGACTATCATCTGATCACTAATCCAGTGATCCACGAGTCAATGCGCTTCTTTATTCGCCATCAACCAGAAAATCTCACCCTGGTGGTGTTGT‑CACGCAACCTTCCGCAACTGGGCATTGCCAATCTGCGTGTTCGTGATCAACTGCTGGAAATTGGCAGTCAGCAACTGGCATTTACCCATCAGGAAGCGAAGCCGTTTTTT‑GATTGTCGTCTGTCa                                                                                                                                                                                                                          <  2:373383/251‑1 (MQ=255)                     ggAATGGCATAGCCCACTTTATCTGGTCATCGATGACTATCATCTGATCACTAATCCAGTGATCCACGAGTCAATGCGCTTCTTTATTCGCCATCAACCAGAAAATCTCACCCTGGTGGTGTTGT‑CACGCAACCTTCCGCAACTGGGCATTGCCAATCTGCGTGTTCGTGATCAACTGCTGGAAATTGGCAGTCAGCAACTGGCATTTACCCATCAGGAAGCGAAGCCGTTTTTT‑GATTGc                                                                                                                                                                                                                                   <  1:116746/241‑1 (MQ=255)                     ggAATGGCATAGCCCACTTTATCTGGTCATCGATGACTATCATCTGATCACTAATCCAGTGATCCACGAGTCAATGCGCTTCTTTATTCGCCATCAACCAGAAAATCTCACCCTGGTGGTGTTGT‑CACGCAACCTTCCGCAACTGGGCATTGCCAATCTGCGTGTTCGTGATCAACTGCTGGAAATTGGCAGTCAGCAACTGGCATTTACCCATCAGGAAGCGAAGCCGTTTTTT‑GATTGc                                                                                                                                                                                                                                   >  2:116746/1‑241 (MQ=255)                     ggAATGGCATAGCCCACTTTATCTGGTCATCGATGACTATCATCTGATCACTAATCCAGTGATCCACGAGTCAATGCGCTTCTTTATTCGCCATAAACCAGAAAATCTCACCCTGGTGGTGTTGT‑CACGCAACCTTCCGCAACTGGGCATTGCCAATCTGAGTGGTCGTGATCAACTGCTGGAAATTGGCAGTCAGCAACTGGCATTTACCCATCAGGAAGCGAAGACGTTTTTT‑GATAGCCGTCTGTCAt                                                                                                                                                                                                                         >  1:137334/1‑251 (MQ=255)                     ggAATGGCATAGCCCACTTTATCTGGTCATCGATGAATATCATCTGATCACTAATCCAGTGATCAACGAGTCAATGCGCTTCTTTATCCGCCATCAACCAGAAAATCTCACCCTGGTGGTGTTGT‑CACGCAACCTTCCGCAACTGGGCATTGCCAATCTGCGTGTTCGTGATCAACTGCTGGAAATTGGCAGTCAGCAACTGGCATTTACCCATCAGGAAGCGAAGCCGTTTTTT‑GATTGCCGTCTGTCAt                                                                                                                                                                                                                         <  1:218865/251‑1 (MQ=255)                             aTAGCCCACTTTATCTGGTCATCGATGACTATCATCTGATCACTAATCCAGTGATCCACGAGTCAATGCGCTTCTTTATTCGCCATCAACCAGAAAATCTCACCCTGGTGGTGTTGT‑CACGCAACCTTCCGCAACTGGGCATTGCCAATCTGCGTGTTCGTGATCAACTGCTGGAAATTGGCAGTCAGCAACTGGCATTTACCCATCAGGAAGCGAAGCCGTTTTTT‑GATTGCCGTCTGTCATCGCCGAtt                                                                                                                                                                                                                 >  1:129442/1‑251 (MQ=255)                             aTAGCCCACTTTATCTGGTCATCGATGACTATCATCTGATCACTAATCCAGTGATCCACGAGTCAATGCGCTTCTTTATTCGCCATCAACCAGAAAATCTCACCCTGGTGGTGTTGT‑CACGCAACCTTCCGCAACTGGGCATTGCCAATCTGCGTGTTCGTGATCAACTGCTGGAAATTGGCAGTCAGCAACTGGCATTTACCCATCAGGAAGCGAAGCCGTTTTTT‑GATTGCCGTCTGTCATCGCCGAtt                                                                                                                                                                                                                 <  1:486843/251‑1 (MQ=255)                                            ttgTCATCGATGACTATCATCTGATCCCTAATCCCATGATCCCCGTATTAATGCGCCTCTTTATTCGCCAACAACCAGAAACTCTCACCCTGGTGG‑GTTTTGCACGCAACCTTCCCCCACTGGGCAATGCCAATCCGCTTGTTCGTTATCAACTGCTGTAAATTGGCCGTTAGGAACTGGGATTTTCCCATCAGGAAACGTATCCGTTTTTTTGATTGCCGTCTGTCATCGCCGATTGAAGCCGCAGaaa                                                                                                                                                                                                    <  1:484760/248‑1 (MQ=255)                                                     aTGACTATCATCTGATCACTAATCCAGTGATCCACGAGTCAATGCGCTTCTTTATTCGCCATCAACCAGAAAATCTCACCCTGGTGGTGTTGT‑CACGCAACCTTCCGCAACTGGGCATTGCCAATCTGCGTGTTCGTGATCAACTGCTGGAAATTGGCAGTCAGCAACTGGCATTTACCCATCAGGAAGCGAAGCCGTTTTTT‑GATTGCCGTCTGTCATCGCCGATTGAAGCCGCAGAAAGCAGTCGGAtt                                                                                                                                                                                         >  1:365421/1‑251 (MQ=255)                                                                  gATCACTAATCCAGTGATCCACGAGTCAATTCGCTTCTTTATTCGCCATCAACCAGAAAATCTCACCCTGGTGGTGTTGT‑CACGCAACCTTCCGCAACTGGGCATTGCCAATCTGCGTGTTCGTGATCAACTGCTGGAAATTGGCAGTCAGCAACTGGCATTTACCCATCAGGAAGCGAAGCCGTTTTTT‑GATTGCCGTCTGTCATCGCCGATTGAAGCCGCAGAAAGCAGTCGGATTTGCGATGAc                                                                                                                                                                                <  1:396064/247‑1 (MQ=255)                                                                    tCACTAATCCAGTGATCCACGAGTCAATGCGCTTCTTTATTCGCCATCAACCAGAAAATCTCACCCTGGTGGTGTTGT‑CACGCAACCTTCCGCAACTGGGCATTGCCAATCTGCGTGTTCGTGATCAACTGCTGGAAATTGGCAGTCAGCAACTGGCATTTACCCATCAGGAAGCGAAGCCGTTTTTT‑GAtt                                                                                                                                                                                                                                     >  1:223145/1‑192 (MQ=255)                                                                    tCACTAATCCAGTGATCCACGAGTCAATGCGCTTCTTTATTCGCCATCAACCAGAAAATCTCACCCTGGTGGTGTTGT‑CACGCAACCTTCCGCAACTGGGCATTGCCAATCTGCGTGTTCGTGATCAACTGCTGGAAATTGGCAGTCAGCAACTGGCATTTACCCATCAGGAAGCGAAGCCGTTTTTT‑GAtt                                                                                                                                                                                                                                     <  2:223145/192‑1 (MQ=255)                                                                    tCACTAATCCAGTGATCCACGAGTCAATGCGCTTCTTTATTCGCCATCAACCAGAAAATCTCACCCTGGTGGTGTTGA‑CACGCAACCTTCCGCACCTGGGCCTTGCCAATCTGCGTGTTCGTGATCAACTGCTGGAAATTGGCAGTCAGCAACTGGCATTTACCCATCAGGAAGCGAAGCCGTTTTTT‑GATTGCCGTCTGTCATCGCCGATTGAAGCCGCAGAAAGCAGTCGGATTTGCGATGACGTTTcc                                                                                                                                                                          >  1:472346/1‑251 (MQ=255)                                                                      aCTAATCCAGTGATCCACGAGTCAATGCGCTTCTTTATTCGCCATCAACCAGAAAATCTCACCCTGGTGGTGTTGT‑CACGCAACCTTCCGCAACTGGGCATTGCCAATCTGCGTGTTCGTGATCAACTGCTGGAAATTGGCAGTCAGCAACTGGCATTTACCCATCAGGAAGCGAAGCCGTTTTTT‑GATTGCCGTCTGTCATCGCCGATTGAAGCCGCAGAAAGCAGTCGGATTTGCGATGACGTTTCCgg                                                                                                                                                                        >  1:76783/1‑251 (MQ=255)                                                                        tAATCCAGTGATCCACGAGTCAATGCGCTTCTTTATTCGCCATCAACCAGAAAATCTCACCCTGGTGGTGTTGT‑CACGCAACCTTCCGCAACTGGGCATTGCCAATCTGCGTGTTCGTGATCAACTGCTGGAAATTGGCAGTCAGCAACTGGCATTTACCCATCAGGAAGCGAAGCCGTTTTTT‑GATTGCCGTCTGTCATCGCCGATTGAAGCCGCAGAAAGCAGTCGGATTTGCGATGACGTTTCCGGtt                                                                                                                                                                      >  1:140530/1‑251 (MQ=255)                                                                              aGTGATCCACGAGTCAATGCGCTTCTTTATTCGCCATCAACCAGAAAATCTCACCCTGGTGGTGTTGT‑CACGCAACCTTCCGCAACTGGGCATTGCCAATCTGCGTGTTCGTGATCAACTGCTGGAAATTGGCAGTCAGCAACTGGCATTTACCCATCAGGAAGCGAAGCCGTTTTTT‑GATTGCCGTCTGTCATCGCCGATTGAAGCCGCAGAAAGCAGTCGGATTTGCGATGACGTTTCCGGTTGGGCg                                                                                                                                                                 >  2:447321/1‑250 (MQ=255)                                                                               gTGATCCACGAGTCAATGCGCTTCTTTATTCGCCATCAACCAGAAAATCTCACCCTGGTGGTGTTGT‑CACGCAACCTTCCGCAACTGGGCATTGCCAATCTGCGTGTTCGTGATCAACTGCTGGAAATTGGCAGTCAGCAACTGGCATTTACCCATCAGGAAGCGAAGCCGTTTTTT‑GATTGCCGTCTGTCATCGCCGATTGAAGCCGCAGAAAGCAGTCGGATTTGCGATGACGTTTCCGGTTGGGCGAc                                                                                                                                                               >  1:439451/1‑251 (MQ=255)                                                                                 gATCCACGAGTCAAGGCGCTTCTTTATTCGCCATCAACCAGAAAATCTCACCCTGGTGGTGTTGT‑CACGCAACCTTCCGCAACTGGGCATTGCCAATCTGCGTGTTCGTGATCAACTGCTGGAAATTGGCAGTCAGCAACTGGCATTTACCCATCAGGAAGCGAAGCCGTTTTGT‑GATTGCCGTCTGTCATCGCCGATTGAAGCCGCAGAAAGCAGTCGGATTTGCGAGGACGTTGCCGGTTGTGCGACg                                                                                                                                                              <  2:472346/250‑1 (MQ=255)                                                                                    ccACGAGTCAATGCGCTTCTTTATTCGACATCAACCAGAAACTCTCACCCTGGTGGTGTTGT‑CACGCAACCTTCCGCAACTGGGCATTGCCAATCTGCGTGTTCGTGATCAACTGCTGGAAATTGGCAGTCAGCAACTGGCATTTACACATCAGGAAGCGAAGCCGTTTTTT‑GATTGCCGTCTGTCATCGCCGATTGAAGCCGCAGAAAGCAGTCGGATTTGCGATGACGTTTCCGGTTGGGCGACGGccc                                                                                                                                                          >  2:448980/1‑249 (MQ=255)                                                                                          gTCAATGCGCTTCTTTATTCGCCATCAACCAGAAAATCTCACCCTGGTGGTGTTGT‑CACGCAACCTTCCGCAACTGGGCATTGCCAATCTGCGTGTTCGTGATCAACTGCTGGAAATTGGCAGTCAGCAACTGGCATTTACCCATCAGGAAGCGAAGCCGTTTTTT‑GATTGCCGTCTGTCATCGCCGATTGAAGCCGCAGAAAGCAGTCGGATTTGCGATGACGTTTCCGGTTGGGCGACGGCACTACAGc                                                                                                                                                    <  1:387690/251‑1 (MQ=255)                                                                                               tGCGCTTCTTTATTCGCCATCAACCAGAAAATCTCACCCTGGTGGTGTTGT‑CACGCAACCTTCCGCAACTGGGCATTGCCAATCTGCGTGTTCGTGATCAACTGCTGGAAATTGGCAGTCAGCAACTGGCATTTACCCATCAGGAAGCGAAGCCGTTTTTT‑GATTGCCGTCTGTCATCGCCGATTGAAGCCGCAGAAAGCAGTCGGATTTGCGATGACGTTTCCGGTTGGGCGACGGCACTACAGCTAATc                                                                                                                                               >  2:201978/1‑251 (MQ=255)                                                                                                         tATTCGCCATCAACCAGAAAATCTCACCCTGGTGGTGTTGT‑CACGCAACCTTCCGCAACTGGGCATTGCCAATCTGCGTGTTCGTGATCAACTGCTGGAAATTGGCAGTCAGCAACTGGCATTTACCCATCAGGAAGCGAAGCCGTTTTTT‑GATTGCCGTCTGTCATCGCCGATTGAAGCCGCAGAAAGCAGTCGGATTTGCGATGACGTTTCCGGTTGGGCGACGGCACTACAGCTAATCGCCCTCTcc                                                                                                                                      <  1:6019/250‑1 (MQ=255)                                                                                                                 aTCAACCAGAAAATCTCACCCTGGTGGTGTTGT‑CACGCAACCTTCCGCAACTGGGCATTGCCAATCTGCGTGTTCGTGATCAACTGCTGGAAATTGGCAGTCAGCAACTGGCATTTACCCATCAGGAAGCGAAGCCGTTTTTT‑GATTGCCGTCTGTCATCGCCGATTGAAGCCGCAGAAAGCAGTCGGATTTGCGATGACGTTTCCGGTTGGGCGACGGCACTACAGCTAATCGCCCTCTCCGCCCGGc                                                                                                                               >  2:372424/1‑249 (MQ=255)                                                                                                                 aTCAACCAGAAAATCTCACCCTGGTGGTGTTGT‑CACGCAACCTTCCGCAACTGGGCATTGCCAATCTGCGTGTTCGTGATCAACTGCTGGAAATTGGCAGTCAGCAACTGGCATTTACCCATCAGGAAGCGAAGCCGTTTTTT‑GATTGCCGTCTGTCATCGCCGATTGAAGCCGCAGAAAGCAGTCGGATTTGCGATGACGTTTCCGGTTGGGCGACGGCACTACAGCTAATCGCCCTCTCCGCCCGGCAg                                                                                                                             <  1:433009/251‑1 (MQ=255)                                                                                                                        aGAAAATCTCACCCTGGAGGTGTTGT‑TACGCAACCTTAAGAAACTGAGCATTGCCAATCTGCGTGTTCGTGATCCACTGCTGGAAATTGGCAGTCAGCAACTGGCATTTACCCATCAGGAAGCGACGCCGTTTTTT‑GATTGCCGTCTGTCATCGCCGATTGAAGCCGCAGCAAGCAGTCGGATTTGCGATGACGTTTCCGGTTGGGCGCCGGCACTACAGCTAATCGCCATCTCCGCCCGGCAGAATAccc                                                                                                                      >  2:225135/1‑251 (MQ=255)                                                                                                                             agctctcccTGGTGGTGTTGT‑CACGCAACCTTCCGCAACTGGGCATTGCCAATCTGCGTGTTCGTGATCAACTGCTGGAAATTGGCAGTCAGCAACTGGCATTTACCCATCAGGAAGCGAAGCCGTTTTTT‑GATTGCCGTCTGTCATCGCCGATTGAAGCCGCAGAAAGCAGTCGGATTTGCGATGACGTTTCCGGTTGGGCGACGGCACTACAGCTAATCGCCCTCTCCGCCCGGCAGAATACCCACTCa                                                                                                                 <  2:192172/245‑1 (MQ=255)                                                                                                                                 cccccTGGTGGTGTTGT‑CACGCAACCTTCCGCAACTGGGCATTGCCAATCTGCGTGTTCGTGATCAACTGCTGGAAATTGGCAGTCAGCAACTGGCATTTACCCATCAGGAAGCGAAGCCGTTTTTT‑GATTGCCGTCTGTCATCGCCGATTGAAGCCGCAGAAAGCAGTCGGATTTGCGATGACGTTTCCGGTTGGGCGACGGCACTACAGCTAATCGCCCTCTCCGCCCGGCAGAATACCCACTCAGcc                                                                                                              <  2:382374/248‑1 (MQ=255)                                                                                                                                     cTGGTGGTGTTGT‑CACGCAACCTTCCGCAACTGGGCATTGCCAATCTGCGTGTTCGTGATCAACTGCTGGCAATTGGCAGTCAGCAACTGGCATTTACCCATCAGGAAGCGAAGCCGTTTTTT‑GATTGCCGTCTGTCATCGCCGATTGAAGCCGCAGAAAGCAGTCGGATTTGCGATGACGTTTCCGGTTGGGCGACGGCACTACAGCTAATCGCCCTCTCCGCCCGGCAGAATACCCACTCAGCCCATa                                                                                                          >  2:445927/1‑250 (MQ=255)                                                                                                                                      tggtggTGTTGT‑CACGCAACCTTCCGCAACTGGGCATTGCCAATCTGCGTGTTCGTGATCAACTGCTGGAAATTGGCAGTCAGCAACTGGCATTTACCCATCAGGAAGCGAAGCCGTTTTTT‑GATTGCCGTCTGTCATCGCCGATTGAAGCCGCAGAAAGCAGTCGGATTTGCGATGACGTTTCCGGTTGGGCGACGGCACTACAGCTAATCGCCCTCTCCGCCCGGCAGAATACCCACTCAGCCCATa                                                                                                          >  2:223916/1‑249 (MQ=255)                                                                                                                                         tggtgTTGT‑CACGCAACCTTCCGCAACTGGGCATTGCCAATCTGCGTGTTCGTGATCAACTGCTGGAAATTGGCAGTCAGCAACTGGCATTTACCCATCAGGAAGCGAAGCCGTTTTTT‑GATTGCCGTCTGTCATCGCCGATTGACGCCGCAGAAAGCAGTCGGATTTGCGATGACGTTTCCGGTTGGGCGCCGGCACTACAGCTAATCGCCCTCTCCGCCCGGCAGAATACCCACTCAGCCCATAAGTCg                                                                                                     >  2:164620/1‑251 (MQ=255)                                                                                                                                          ggtgTTGT‑CACGAAACCTTCCGCCACTGGGCATTGCCACTCTGCGTGTTCGTGATCAACTGCTGGAAATTGGCAGTCAGCAACTGGCATTTACCCATCAGGAAGCGAAGCCGTTTTTT‑GATTGCCGTCTGTCATCGCCGATTGAAGCCGCAGAAAGCAGTCGGATTTGCGATGACGTTTCCGGTTGGGCGACGGCACTACCGCTAATCGCCCTCTCCGCCCGGCAGAATACCCACTCCGCCCATAAGTCgg                                                                                                    >  2:316975/1‑251 (MQ=255)                                                                                                                                             gttgt‑CACGCAACCTTCCGCAACTGGGCATTGCCAAACTGCGTGTTCGTGATCAACTGCTGGAAATTGGCAGTCAGCAACTGGCATTTACCCATCAGGAAGCGAAGCCGTTTTTT‑GATTGCCGTCTGTCATCGCCGATTGAAGCCGCAGAAAGCAGTCGGATTTGCGATGACGTTTCCGGTTGGGCGACGGCACTACAGCTAATCGCCCTCTCCGCCCGGCAGAATACCCACTCAGCCCATAAGTCGGCAc                                                                                                 >  2:33958/1‑251 (MQ=255)                                                                                                                                                     cGCAACCTTCCGCAACTGGGCATTGCCAATCTGCGTGTTCGTGATCAACTGCTGGAAATTGGCAGTCAGCAACTGGCATTTACCCATCAGGAAGCGAAGCCGTTTTTT‑GATTGCCGTCTGTCATCGCCGATTGAAGCCGCAGAAAGCAGTCGGATTTGCGATGACGTTTCCGGTTGGGCGACGGCACTACAGCTAATCGCCCTCTCCGCCCGGCAGAATACCCACTCAGCCCATAAGTCGGCACGCCGCCt                                                                                          <  2:120245/251‑1 (MQ=255)                                                                                                                                                        aaCCCTCCGCAACTGGGCAATGCCAATCTGGGTGTTCGTGATCAACTGCTGGAAATTGGCAGTCAGCAACTGGCAGTTACCCATCAGGAAGTGAAGCCGTTTTTT‑GATTGCCGTCTGTCATCGCCGAGTGAAGCCGCAGAAAGCAGTCGGATTTGCGATGACGTTTCCGGTTGGGCGGCGGCACTACAGCTAATCGCCCTCTCCGCCCGGCAGAATACCCACTCAGCCCATAAGTCGGCACGCCGCCTGGc                                                                                       <  1:32469/251‑1 (MQ=255)                                                                                                                                                             tCCGCAACTGGGCATTGCCAATCTGCGTGTTCGTGATCAACTGCTGGAAATTGGCAGTCAGCAACTGGCATTTACCCATCAGGAAGCGAAGCCGTTTTTT‑GATTGCCGTCTGTCATCGCCGATTGAAGCCGCAGAAAGCAGTCGGATTTGCGATGACGTTTCCGGTTGGGCGACGGCACTACAGCTAATCGCCCTCTCCGCCCGGCAGAATACCCACTCAGCCCATAAGTCAGCACGCCGCCTGGCgggc                                                                                   >  1:265270/1‑249 (MQ=255)                                                                                                                                                                 cAACTGGGCATTGCCAATCTGCGTGTTCGTGATCAACTGCTGGAAATTGGCAGTCAGCAACTGGCATTTACCCATCAGGAAGCGAAGCCGTTTTTT‑GATTGCCGTCTGTCATCGCCGATTGAAGCCGCAGAAAGCAGTCGGATTTGCGATGACGTTTCCGGTTGGGCGACGGCACTACAGCTAATCGCCCTCTCCGCCCGGCAGAATACCCACTCAGCCCATAAGTCGGCACGCCGCCTGGCGGGAATCa                                                                               >  2:167571/1‑250 (MQ=255)                                                                                                                                                                     tGGGCATTGCCAATCTGCGTGTTCGTGATCAACTGCTGGAAATTGGCAGTCAGCAACTGGCATTTACCCATCAGGAAGCGAAGCCGTTTTTT‑GATTGCCGTCTGTCATCGCCGATTGAAGCCGCAGAAAGCAGTCGGATTTGCGATGACGTTTCCGGTTGGGCGACGGCACTACAGCTAATCGCCCTCTCCGCCCGGCAGAATACCCACTCAGCCCATAAGTCGGCACGCCGCCTGGCGGGAATCAATgcc                                                                          >  1:354505/1‑251 (MQ=255)                                                                                                                                                                          aTTGCCAATCTGCGTGTTCGTGATCAACTGCTGGAAATTGGCAGTCAGCAACTGGCATTTACCCATCAGGAAGCGAAGCCGTTTTTT‑GATTGCCGTCTGTCATCGCCGATTGAAGCCGCAGAAAGCAGTCGGATTTGCGATGACGTTTCCGGTTGGGCGACGGCACTACAGCTAATCGCCCTCTCCGCCCGGCAGAATACCCACTCAGCCCATAAGTCGGCACGCCGCCTGGCGGGAATCAATgccagcc                                                                      >  1:15588/1‑250 (MQ=255)                                                                                                                                                                              ccAATCTGCGTGTTCGTGCACAACTGCGGGAAAGTGGCAGTCAGCAACGGGCATTTACCCATCAGGAAGCGAAGCCGTTTTTT‑GATTGCCGTCTGTCATCGCCGATTGAAGCCGCAGAAAGCAGTCGGATTTGCGATGACGTTTCCGGTTGGGCGACGGCACTACAGCTAATCGCCCTCTCCGCCCGGCAGAATACCCACTCAGCCCATAAGGCGGCGTGCCGCCTGGCGGGAATCAATGCCAGCCATCtt                                                                 <  2:87504/251‑1 (MQ=255)                                                                                                                                                                                aaTCTGCGTGTTCGTGATCAACTGCTGGAAATTGGCAGTCAGCAACTGGCATTTACCCATCAGGAAGCGAAGCCGTTTTTT‑GATTGCCGTCTGTCATCGCCGATTGAAGCCGCAGAAAGCAGTCGGATTTGCGATGACGTTTACGGTTGGGCGACGGCACTACAGCTAATCGCCCTCTCCGCCCGGCAGAATACCCACTCATCCCATAAGTCGGCACGCCGCCTGGCGGGAATCAATGCCAGCCATCTTTc                                                               >  1:212860/1‑251 (MQ=255)                                                                                                                                                                                 aTCTGCGTGTTCGTGATCAACTGCTGGAAATTGGCAGTCAGCAACTGGCATTTACCCATCAGGAAGCGAAGCCGTTTTTT‑GATTGCCGTCTGTCATCGCCGATTGAAGCCGCAGAAAGCAGTCGGATTTGCGATGACGTTTCCGGTTGGGCGACGGCACTACAGCTAATCGCCCTCTCCGCCCGGCAGAATACCCACTCAGCCCATAAGTCGGCACGCCGCCTGGCGGGAATCAATGCCAGCCATCTTTCg                                                              >  1:251231/1‑251 (MQ=255)                                                                                                                                                                                  tCTGCGTGTTCGTGATCAACTGCTGGAAATTGGCAGTCAGCAACTGGCATTTACCCATCAGGAAGAGAAGCCGTTTTTT‑GATTGCCGTCTGTCATCGCCGATTAAAGCCGCAGAAAGCAGTCGGATTTGCGATGACGTTTCCGCTAGGGCAACGGCACTACAGCTAATCGCCCTCTCCGCCCGGCAGAATACCCACTCAGCCCATAAGTCGGCAAGCCGCCTGGCGGGAATCAATGCCAGCCATCTTTCgg                                                             >  1:207551/1‑251 (MQ=255)                                                                                                                                                                                     gCGTGTTCGTGATCAACTGCTGGAAATTGGCAGTCAGCAACTGGCATTTACCCATCAGGAAGCGAAGCCGTTTTTT‑GATTGCCGTCTGTCATCGCCGATTGAAGCCGCAGAAAGCAGTCGGATTTGCGATGACGTTTCCGGTTGGGCGACGGCACTACAGCTAATCGCCCTCTCCGCCCGGCAGAATACCCACTCAGCCCATAAGTCGGCACGCCGCCTGGCGGGAATCAATGCCAGCCATCTTTCGGa                                                            <  2:186552/249‑1 (MQ=255)                                                                                                                                                                                     gCGTGTTCGTGATCAACTGCTGGAAATTGGCAGTCAGCAACTGGCATTTACCCATCAGGAAGCGAAGCCGTTTTTT‑GATTGCCGTCTGTCATCGCCGATTGAAGCCGCAGAAAGCAGTCGGATTTGCGATGACGTTTCCGGTTGGGCGACGGCACTACAGCTAATCGCCCTCTCCGCCCGGCAGAATACCCACTCAGCCCATAAGTCGGCACGCCGCCTGGCGGGAATCAATGCCAGCCATCTTTCGGa                                                            <  1:255055/249‑1 (MQ=255)                                                                                                                                                                                     gCGTGTTCGTGATCAACTGCTGGAAATTGGCAGTCAGCAACTGGCATTTACCCATCAGGAAGCGAAGCCGTTTTTT‑GATTGCCGTCTGTCATCGCCGATTGAAGCCGCAGAAAGCAGTCGGATTTGCGATGACGTTTCCGGTTGGGCGACGGCACTACAGCTAATCGCCCTCTCCGCCCGGCAGAATACCCACTCAGCCCATAAGTCGGCACGCCGCCTGGCGGGAATCAATGCCAGCCATCTTTCGGAtt                                                          <  1:12586/251‑1 (MQ=255)                                                                                                                                                                                     gCGTGTTCGTGATCAACTGCTGGAAATTGGCAGTCAGCAACTGGCATTTACCCATCAGGAAGCGAAGCCGTTTTTT‑GATTGCCGTCTGTCATCGCCGATTGAAGCCGCAGAAAGCAGTCGGATTTGCGATGACGTTTCCGGTTGGGCGACGGCACTACAGCTAATCGCCCTCTCCGCCCGGCAGAATACCCACTCAGCCCATAAGTCGGCACGCCGCCTGGCGGGAATCAATGCCAGCCATCTTTCGGAtt                                                          <  1:372424/251‑1 (MQ=255)                                                                                                                                                                                     gCGTGTTCGTGACCAACTGCTGGAAATTGGCAGTCAGCAACTGGCATTTACCCATCAGTAAGCGAAGCCGTTTTTG‑GATTGCCGTCTGGCATCGCCTATTGAAGCCGCAGAAAGCAGTCGGATTTGCGATGACGTTTCCGGTTGGGCGACGGCACTACAGCTAATCGCTCTCTCCGCCCGGCAGAATACCCCCTCAGCCCATAAGTCGGCACGCCGCCTGGCGGGAATCAATGCCAGCCATCTTTCgg                                                             <  2:421377/248‑1 (MQ=255)                                                                                                                                                                                      cGTGTTCGTGATCAACTGCTGGAAATTGGCAGTCAGCAACTGGCATTTACCCATCAGGAAGCGAAGCCGTTTTTT‑GATTGCCGTCTGTCATCGCCGATTGAAGCCGCCGAAAGCAGTCGGATTTGCGATGACGTTTCCGGTTGGGCGACGGCACTACAGCTAATCGCCCTCTCCGCCCGGCAGAATACCCACTCAGCCCATAAGTCGGCACGCCGCCTGGCGGGAATCAATGCCAGCCATCTTTCGGAtt                                                          >  1:439500/1‑250 (MQ=255)                                                                                                                                                                                              tGATCAACTGCTGGAAATTGGCAGTCAGCAACTGGCATTTACCCATCAGGCAGCGAAGCCGTTTTTT‑GATTGCCGTCTGTCATCGCCGATTGAAGCCGCAGAAAGCAGTCGGATTTGCGATGACGTTTCCGGTTGGGCGACGGCACTACAGCTAATCGCCCTCTCCGCCCGGCAGAATACCCACTCAGCCCATAAGTCGGCACGCCGCCTGGCGGGAATCAATGCCAGCCATCTTTCGGATTATCTGGTCg                                                 >  1:475626/1‑251 (MQ=255)                                                                                                                                                                                                          ggAAATTGGCAGTCAGCAACTGGCATTTACCCATCAGGAAGCGAAGCCGTTTTTT‑GATTGCCGTCTGTCATCGCCGATTGAAGCCGCAGAAAGCAGTCGGATTTGCGATGACGTTTCCGGTTGGGCGACGGCACTACAGCTAATCGCCCTCTCCGCCCGGCAGAATACCCACTCAGCCCATAAGTCGGCACGCCGCCTGGCGGGAATCAATGCCAGCCATCTTTCGGATTATCTGGTCGATGAGGtttt                                       <  1:337949/249‑1 (MQ=255)                                                                                                                                                                                                          ggAAATTGGCAGTCAGCAACTGGCATTTACCCATCAGGAAGCGAAGCCGTTTTTT‑GATTGCCGTCTGTCATCGCCGATTGAAGCCGCAGAAAGCAGTCGGATTTGCGATGACGTTTCCGGTTGGGCGACGGCACTACAGCTAATCGCCCTCTCCGCCCGGCAGAATACCCACTCAGCCCATAAGTCGGCACGCCGCCTGGCGGGAATCAATGCCAGCCATCTTTCGGATTATCTGGTCGATGAGGTTTTg                                      <  2:33051/250‑1 (MQ=255)                                                                                                                                                                                                             aaTTGGCAGTCAGCAACTGGCATTTACCCATCAGGAAGCGAAGCCGTTTTTT‑GATTGCCGTCTGTCCTCGCCGATTGACGCCGCAGCCAGCAGTCGGATTTGCGATGACGTTTCCGGTTGGGCGACGGCACTACAGCTAATCGCCCTCTCCGCCCGGCAGAATCCCCACTCAGCCCATAAGTCGGCACGCCGCCTGGCGGGAATCAATGCCAGCCATATTTCGGCTTATCTGGTCGATGAGGTTTTGGAt                                   >  1:326597/1‑250 (MQ=255)                                                                                                                                                                                                              aTTGGCAGTCAGCAACTGGCATTTACCCATCAGGAAGCGAAGCCGTTTTTT‑GATTGCCGTCTGTCATCGCCGATTGAAGCCGCAGAAAGCAGTCGGATTTGCGATGACGTTTCCGGTTGGGCGACGGCACTACAGCTAATCGCCCTCTCCGCCCGGCAGAATACCCACTCAGCCCATAAGTCGGCACGCCGCCTGGCGGGAATCAATGCCAGCCATCTTTCGGATTATCTGGTCGATGAGGTTTTGGATaa                                 <  1:136285/251‑1 (MQ=255)                                                                                                                                                                                                                 ggCAGTCAGCAACTGGCATTTACCCATCAGGAAGCGAAGCCGTTTTTT‑GATTGCCGTCTGTCATCGCCGATTGAAGCCGCAGAAAGCAGTCGGATTTGCGATGACGTTTCCGGTTGGGCGACGGCACTACAGCTAATCGCCCTCTCCGCCCGGCAGAATACCCACTCAGCCCATAAGTCGGCACGCCGCCTGGCGGGAATCAATGCCAGCCATCTTTCGGATTATCTGGTCGATGAGGTTTTGGCTAACGt                              >  1:231813/1‑251 (MQ=255)                                                                                                                                                                                                                  gaagacaacAACTGACATATACCCATCCGGAAGCGAAGCCGCTTTTT‑GAATGCCGTCTGTCATCGCCGATTGAAGCCGCAGAAAGCAGTCGGATTTGAGATGACGTTTCCGGTTGGGCGACGGCACTACAGATAATCGCCCTCTCCGCCAGGCAGAATACCCACTCAGCCCATAAGTCGGCCAGCCGCCTGGCGGGAATCAATGCCAGCCCTCTTTGGGGTTATCTGGTCGATGTGGTTTTGGATAACGtg                             >  2:302615/9‑250 (MQ=255)                                                                                                                                                                                                                                      aCCCATCAGGAAGCGAAGCCGTTTTTT‑GATTGCCGTCTGTCATCGCCGATTGAAGCCGCAGAAAGCAGTCGGATTTGCGATGACGTTTCCGGTTGGGCGACGGCACTACAGCTAATCGCCCTCTCCGCCCGGCAGAATACCCACTCAGCCCATAAGTCGGCACGCCGCCTGGCGGGAATCAATGCCAGCCATCTTTCGGATTATCTGGTCGATGAGGTTTTGGATAACGTCGATCTCGCAACGCGCCAttt         >  2:156007/1‑251 (MQ=255)                                                                                                                                                                                                                                              ggAAGAGAAGCCGTTTTTT‑GATTGCCGTCTGTCATCGCCGATTGCAGCCGCAGAAAGCAGTCGGATTTGCGATGACGTTTCCGGTTGGGCGACGGCACTACAGCTAATCGCCCTCTCCGCACGGCAGAATACCCACTCAGCCCATAAGTCGGCACGCCGCCTGGCGGGAATCAATGCCAGCCCTCTTTCGGATTATCTGGTCGCTGAGGTTTTGGATAACGTCGATCTCCCAACGTGCCATTTTCTGTTg  >  2:354369/1‑250 (MQ=255)                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                CAGCTTTTCATTGAGCTGGCGGAATGGCATAGCCCACTTTATCTGGTCATCGATGACTATCATCTGATCACTAATCCAGTGATCCACGAGTCAATGCGCTTCTTTATTCGCCATCAACCAGAAAATCTCACCCTGGTGGTGTTGT‑CACGCAACCTTCCGCAACTGGGCATTGCCAATCTGCGTGTTCGTGATCAACTGCTGGAAATTGGCAGTCAGCAACTGGCATTTACCCATCAGGAAGCGAAGCAGTTTTTT‑GATTGCCGTCTGTCATCGCCGATTGAAGCCGCAGAAAGCAGTCGGATTTGCGATGACGTTTCCGGTTGGGCGACGGCACTACAGCTAATCGCCCTCTCCGCCCGGCAGAATACCCACTCAGCCCATAAGTCGGCACGCCGCCTGGCGGGAATCAATGCCAGCCATCTTTCGGATTATCTGGTCGATGAGGTTTTGGATAACGTCGATCTCGCAACGCGCCATTTTCTGTTG  >  NC_000913/3553415‑3553900

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑