Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	284,535	T→G	A445A (GCT→GCG)	yagF →	D‑xylonate dehydratase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	284,535	0	T	G	100.0%	204.8 / NA	68	A445A (GCT→GCG)	yagF	D‑xylonate dehydratase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (26/42);  total (26/42)

GCGGCGTGCCGGAGGTGATGCTCCACCTG‑CGCGACCTCGGCCTGCTGCATCTGGACGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCTCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGAAGAGATTGTGCAGGGCGATATCATGGTGGTGATCGGCGGCGGGCCGTCCGGCACCGGCATGGAAGAGACCTACCAGCTCACCTCCGCGCTAAAGCATATCTCGTGGGGCA  >  NC_000913/284288‑284776                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                  gcggcgTGCCGGAGGTGATGCTCCACCTG‑CGCGACCTCGGCCTGCTGCATCTGGACGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCg                                                                                                                                                                                                                                                <  1:375105/251‑1 (MQ=255)        gCCGGAGGTGATGCTCCACCTG‑CGCGACCTCGGCCTGCTGCATCTGGCCGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCCGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGtt                                                                                                                                                                                                                                         >  2:187330/1‑251 (MQ=255)        cccGGAGGTGTTGCTCC‑CCTGGCGCGACCTCGGCCTGCTGCATCGGGCCGCAATGACCGTGCCCGGCCAGGCGGTGGGCGAGACCCTTGATTGGGGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGTATGACGTGAGCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTTCCGCGGGGCAACAGCGCGCCGGAAGGtt                                                                                                                                                                                                                                         <  2:59523/250‑1 (MQ=255)          cGGAGGTGATGCTCCACCTG‑CGCGACCTCGGCCTGCTGCATCTGGACGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGCAGGTTCg                                                                                                                                                                                                                                       >  1:224246/1‑251 (MQ=255)            gAGGGGATGCTCCACCCG‑CGCGACCTCGTCCTGCTGCATCTGGACGCCATGACCGTGACCGGCCAGACGGTGGGCGAGACCCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCTCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGt                                                                                                                                                                                                                                     <  1:61722/251‑1 (MQ=255)                      cccACCTG‑CGCGACCTCGGCCTGCTGCATCTGGACGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGcc                                                                                                                                                                                                                           <  1:187330/250‑1 (MQ=255)                           ccggcgcgagccacgccggcggccgcTGGACGGCCTGACCGGGATCGTCCAGCCGGTGGGTGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCGGGGCGCGCTGCCGCCAGTGCCTGCGCGAGCAGGACGGCGGAGAGCCGGATGACGTGATCCTGCCGGCGGAGAAGGCAAAAGCGAAAGGGCCGGCCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTGCGGGGATGAAGCCCACgg                                                                                                                                                                                                                       <  2:199058/226‑1 (MQ=255)                             g‑cgcGACCTCGGCCTGCTGCATCTGGACGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGa                                                                                                                                                                                                                    <  1:22115/251‑1 (MQ=255)                                gcgACCTCGGCCTGCTGCATCTGGACGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATc                                                                                                                                                                                                                  <  1:288347/251‑1 (MQ=255)                                gcgACCCCGGCCTGCTGCATCTGGACGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATc                                                                                                                                                                                                                  <  1:318225/251‑1 (MQ=255)                                 cgACCTCGGCCTGCTGCATCTGGACGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCg                                                                                                                                                                                                                 <  1:243176/251‑1 (MQ=255)                                       cGGCCTGCTGCATCTGGACGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGt                                                                                                                                                                                                           >  1:163589/1‑251 (MQ=255)                                             gctgctTCTGGCCGACATGACCGTGACCGGCCAGCCGGTGGGCGAGAGCCTTGAATGGAGGCCGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGAGCCTGCGCGAGCAGGACGGCATAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCACGCCGGGCAACATAGCGCCGGAAGGATCGGTGATCAAGGCCACGGCGCTCGACCCGTCggtgg                                                                                                                                                                                                     >  2:77325/1‑251 (MQ=255)                                                      ggACGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCg                                                                                                                                                                                               <  1:318101/248‑1 (MQ=255)                                                          gCCATGACCGTGACCGGCCAGGCGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATgg                                                                                                                                                                                        <  1:274842/251‑1 (MQ=255)                                                                     gaccgGCCAGACGGGGGGAGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCGGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGGCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATaccacc                                                                                                                                                                             <  1:153004/251‑1 (MQ=255)                                                                          cccAGACGGGGGGGGGGACCCTTGACTGGTGGCAGGCGTCCGGGCGCCGGGCGGGCGTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGAGGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTGCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGAGCGACCCGTCGGTGGTTGGCGAAGAGGGCGTAGACTACCACAcc                                                                                                                                                                        <  2:186250/250‑1 (MQ=255)                                                                              gACGGTGGGCGAGATCCTGGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTTACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGATGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGTAGATGGCGTATGCCACCACAccggcc                                                                                                                                                                    <  2:217680/251‑1 (MQ=255)                                                                                     ggCGGGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGTTGCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGATAAGGCAAAAGCGAAAGGGCGGACCTCGACGGTCTGCTTCCCGACTGGCAGCAGCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGGGGTGGGCGAAGAGGGCGTTTACCGCCACACCTGCcgggtgcg                                                                                                                                                             <  2:65969/251‑1 (MQ=255)                                                                                       cGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCcgggtgcggg                                                                                                                                                           <  1:480212/251‑1 (MQ=255)                                                                                          gaACCTTGAATGGTGGCAGGCGGCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGgtgt                                                                                                                                                        <  1:425487/251‑1 (MQ=255)                                                                                           aaCCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGtt                                                                                                                                                       >  1:230736/1‑251 (MQ=255)                                                                                                tGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGtct                                                                                                                                                  >  1:182904/1‑251 (MQ=255)                                                                                                 gaagggtggCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATGCCACCACACCGGCCGGGTGCGGGTGTTTGtctc                                                                                                                                                 <  1:395912/247‑1 (MQ=255)                                                                                                 gAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGACGCCGGAGACGGCAAAAGCGAACGGGCTGACCCCGACGGTCTGCTTCCCGACGGGCAACATCGCGACGGAAGGTTCGGTGCTCACGGCCACGGCGATCGACCCGTCGGTGGGGGGCGAAGATGGCGTATAACACCACACCGGCCGGGAGCGGGGGTTTGgctc                                                                                                                                                 >  2:353768/1‑251 (MQ=255)                                                                                                     gggggCACGCGTCCGGGCCGCGGGCGCGCTTCCGCCATTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGGTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGGGGTGGGCGAAGATGGCGTAGACCCCCACACCGTCCGGGGGCTGGTGTTTGTCTCTGaa                                                                                                                                             <  2:452258/248‑1 (MQ=255)                                                                                                          cAGGCGTGCGAGCGACCGGCGCGCTTCCGCCAGTGCCTGCGCGAGCCGGACGGCGCAGAGCCGGAGGCCGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAAGATCGCGCCGGCCGGTTCGGTGCTCAAGGCCACGGCGCTCGACCCGTCGGAGGTGGGGGAAGATGGCGGAACCCACAACACCGGCCGGGGGCGGGTGTTTGTCGCGGAAgcgc                                                                                                                                         >  2:162067/1‑250 (MQ=255)                                                                                                          cAGGCGGCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCa                                                                                                                                        <  2:140708/251‑1 (MQ=255)                                                                                                           aGGCGTCAGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCACAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGAGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGAAGGCGTATACCACCACCCCGGCCGGGTGCGGGGGTTTGTCTCGGAAgcgc                                                                                                                                         >  2:496260/1‑249 (MQ=255)                                                                                                               gTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGCCGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCaggcg                                                                                                                                    >  1:301929/1‑250 (MQ=255)                                                                                                                 ccGAGCGCCGGGCGCGGTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCaggcgatc                                                                                                                                 <  1:96837/251‑1 (MQ=255)                                                                                                                 ccGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGTAAGCGCaggcgatc                                                                                                                                 <  1:143017/251‑1 (MQ=255)                                                                                                                     gcgcCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCaggcgatcaagg                                                                                                                             <  1:344017/251‑1 (MQ=255)                                                                                                                         cGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCaggcgatcaaggcgat                                                                                                                         <  1:348441/251‑1 (MQ=255)                                                                                                                             cgcgcTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGGGCGGGGGTTTGTCTCGGAAGCGCaggcgatcaaggcgatca                                                                                                                       >  2:99466/1‑249 (MQ=255)                                                                                                                                    ccGCCAGTGCGTGCGCGAGCAGGACGGCGTAGATCCGGATGACGTTATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGAag                                                                                                              <  1:446960/251‑1 (MQ=255)                                                                                                                                        cAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGAAGAGAt                                                                                                          <  1:65405/251‑1 (MQ=255)                                                                                                                                           ggCCTGCGCGAGCAGGAGGGCGTAGAGCCGGATGACGTGATCCTGCCGCCTGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGTCCCGTCGGTGGTGGTCGAAGATGTCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGAAGAGATtgt                                                                                                       <  1:336881/250‑1 (MQ=255)                                                                                                                                               tGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGAAGAGATTGTGc                                                                                                     >  1:62396/1‑249 (MQ=255)                                                                                                                                                  gggagaaggccggcggAGAGCCGGATGCCGGGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGGCCGCCACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGCTCAAGGCGATCAAGCGGGAATAGATTGTGCAgagc                                                                                                <  2:46416/241‑4 (MQ=255)                                                                                                                                                          gACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGAAGAGATTGTGCAGGGCGATATCAt                                                                                        <  1:344550/251‑1 (MQ=255)                                                                                                                                                              gCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGAAGAGATTGTGCAGGGCGATATCAtggtg                                                                                    <  1:171087/251‑1 (MQ=255)                                                                                                                                                              gCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGAAGAGATTGTGCAGGGCGATATCAtggtg                                                                                    <  1:29352/251‑1 (MQ=255)                                                                                                                                                               cGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGAAGAGATTGTGCAGGGCGATATCAtggtgg                                                                                   >  1:408284/1‑251 (MQ=255)                                                                                                                                                               cGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGAAGAGATTGTGCAGGGCGATATCAtggtgg                                                                                   <  1:364010/251‑1 (MQ=255)                                                                                                                                                                  agagCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGAAGAGATTGTGCAGGGCGATATCATGgtggtg                                                                                 <  1:222916/250‑1 (MQ=255)                                                                                                                                                                    agCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGGCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGAAGAGATTGTGCAGGGCGATATCATGGTGGTGATc                                                                              <  1:376872/251‑1 (MQ=255)                                                                                                                                                                      ccGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGACAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGCGCGGGTGTTTGTCTCGGAAGCGCCGGCGATCAAGGCGATCAAGCGGGAAGAGATTGTGCAGGGCGATATCATGGGGGGGATcgg                                                                            >  2:357008/1‑251 (MQ=255)                                                                                                                                                                               gTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGGCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGCGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGAAGAGATTGTGCAGGGCGATATCATGGTGGTGATCGGCGGCGGGcc                                                                   >  1:134847/1‑251 (MQ=255)                                                                                                                                                                                  aTCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGCGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGAAGAGATTGTGCAGGGCGATATCATGGTGGTGATCGGCGGCGGGCCGTc                                                                >  1:317704/1‑251 (MQ=255)                                                                                                                                                                                    ccTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGAAGAGATTGTGCAGGGCGATATCATGGTGGTGATCGGCGGCGGGCCGTccg                                                              >  2:380238/1‑251 (MQ=255)                                                                                                                                                                                       gccgccGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGAAGAGATTGTGCAGGGCGATATCATGGTGGTGATCGGCGGCGGGCCGTccggca                                                           <  1:94145/251‑1 (MQ=255)                                                                                                                                                                                              gagaAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGAAGAGATTGTGCAGGGCGATATCATGGTGGTGATCGGCGGCGGGCCGTCCGGCACCGGCAt                                                    <  1:376908/251‑1 (MQ=255)                                                                                                                                                                                               agaAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGGGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGAAGAGATTGTGCAGGGCGATATCCTGGTGGTGATCGGCGGCGGGGCGGCCGGCAACGGCATg                                                   >  2:485241/1‑251 (MQ=255)                                                                                                                                                                                                gaAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGCTCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGAAGAGATTGTGCAGGGCGATATCATGGTGGTGATCGGCGGCGGGGCGTCCGGCGCCGGCGTgg                                                  >  2:247263/1‑251 (MQ=255)                                                                                                                                                                                                    gCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGTTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGAGGGCGTATACCACCACTCCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGAAGAGATTGTGCAGGGCGATATCATGGTGGTGATCGGCGGCGGGCCGTCCTGCACCGGCATGGAaga                                              <  2:158992/251‑1 (MQ=255)                                                                                                                                                                                                      aaaaGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGAAGAGATTGTGCAGGGCGATATCATGGTGGTGATCGGCGGCGGGCCGTCCGGCACCGGCATGGAag                                               >  1:31746/1‑248 (MQ=255)                                                                                                                                                                                                           cGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGAAGAGATTGTGCAGGGCGATATCATGGTGGTGATCGGCGGCGGGCCGTCCGGCACCGGCATGGAAGAGACCTAc                                       >  1:77492/1‑251 (MQ=255)                                                                                                                                                                                                              aaGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGAAGAGATTGTGCAGGGCGATATCATGGTGGTGATCGGCGGCGGGCCGTCCGGCACCGGCATGGAAGAGACCTAcc                                      >  1:267354/1‑249 (MQ=255)                                                                                                                                                                                                              aaGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGAAGAGATTGTGCAGGGCGATATCATGGTGGTGATCGGCGGCGGGCCGTCCGGCACCGGCATGGAAGAGACCTACCAg                                    <  2:105576/251‑1 (MQ=255)                                                                                                                                                                                                                            ccGGTCTGCTGCCCGACGGGCAACACCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGTGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGAAGAGATTGTGCAGGGCGATATCATGGTGGTGATCGGCGGCGGGCCGTCCGGCACCGGCATGGAAGAGACCTACCAGCTCACCTCCGCGCt                      <  2:19575/250‑1 (MQ=255)                                                                                                                                                                                                                             cGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGAAGAGATTGTGCAGGGCGATATCATGGTGGTGATCGGCGGCGGGCCGTCCGGCACCGGCATGGAAGAGACCTACCAGCTCACCGCCGCGCTa                     <  2:294095/251‑1 (MQ=255)                                                                                                                                                                                                                                  tGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGACCCCGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGACGCGCAGGCGATCCAGGCGATCAAGCGGGAAGAGATTCTGCAGGGCGATATCATGGTGGTGATCGGCGGCGGGCCGTCCGGCACCGGGATAGAAGGGACCTGACAGCTCAcccccccgcctaaga                 >  2:21176/1‑237 (MQ=255)                                                                                                                                                                                                                                   gCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGAAGAGATTGTGCAGGGCGATATCATGGTGGTGATCGGCGGCGGGCCGTCCGGCACCGGCATGGAAGAGACCTACCAGCTCACCTCCGCTCTAAAg                  <  1:389424/248‑1 (MQ=255)                                                                                                                                                                                                                                   gCTTCCCGACGGGCAACAGCGCGCCGGAAGGTGCGGTGATCAAGGCCGCGGCGATCGCCCCGGCGGTGGTGGGCGAAGATGGCGTATACCTCCACACCGGCCGGGTGCGGGTGTTGGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGAAGAGATTGTGCAGGGCGATATCATGGTGTTGATCGGCTGCGGGCCGTCCGGCACCGGGATGGAAGAGACCTACCAGCCTACCTCCGCGCTAAAGc                 <  2:240049/249‑1 (MQ=255)                                                                                                                                                                                                                                       cccGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGAAGAGATTGTGCAGGGCGATATCATGGTGGTGATCGGCGGCGGGCCGTCCGGCACCGGCATGGAAGAGACCTACCAGCTCACCTCCGCGCTAAAGCATAtct           >  1:230946/1‑251 (MQ=255)                                                                                                                                                                                                                                            cGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGAAGAGATTGTGCAGGGCGATATCATGGTGGGGATCGGCGGCGGGCCGTCCGGCACCGGCATGGAAGAGACCTACCAGCTCACCTCCGCGCTAAAGCATATCTCgggg      >  2:442906/1‑248 (MQ=255)                                                                                                                                                                                                                                                 aaCATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGAAGAGATTGTGCAGGGCGATATCATGGTGGTGATCGGCGGCGGGCCGTCCGGCACCGGCATGGAAGAGACCTACCAGCTCACCTCCGCGCTAACGCATATCTCGTGGGGCa  >  1:100938/1‑250 (MQ=255)                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                  GCGGCGTGCCGGAGGTGATGCTCCACCTG‑CGCGACCTCGGCCTGCTGCATCTGGACGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCTCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGAAGAGATTGTGCAGGGCGATATCATGGTGGTGATCGGCGGCGGGCCGTCCGGCACCGGCATGGAAGAGACCTACCAGCTCACCTCCGCGCTAAAGCATATCTCGTGGGGCA  >  NC_000913/284288‑284776

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑