Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,042,171 G→A intergenic (+146/‑197) zinT → / → yodB metal‑binding protein ZinT/putative cytochrome b561 YodB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,042,1710GA93.8% 54.3 / NA 16intergenic (+146/‑197)zinT/yodBmetal‑binding protein ZinT/putative cytochrome b561 YodB
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (6/9);  minor base C (1/0);  total (7/9)
Fisher's exact test for biased strand distribution p-value = 4.38e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CACAGCAATCTTTATTAAATGAAATGGAAAACTGGCCAACGTATTATCCATATCAGTTGAGTAGCGAAGAAGTGGTCGAGGAAATGATGTCTCATTGAGTATTCTCATGATAACGCCTCGATGCCGCTTTAGTAAGTTATCATAACTGCCACTGGTCCATCCACAAACGCCACTGAACGCAAGCTAGCTACAGACACGCTCATCACTATGACGTGTCTGTATATTAATAAGCTAACCCGCATTGAGTTAACCAATAACGGATTCCATACACAATACGGCCAACAGAGAAAGATACCTGTGCTCACGCCATTGCTTATATTGGCTGGTTACAATATGCACTATCAATTTTTTAAATAACAAATTATTAAAACCACATCTGTATAAGGAATTTTTAAGGTTCGTGGGATAGCTTGACTGTGAAAATCACAGGAGCTACAAAAATGAACCGATTCTCAAAAACTCA  >  NC_000913/2041928‑2042390
                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                           
cacaGCAATCTTTATTAAATGAAATGGAAAACTGGCCAACGTATTATCCATATCAGTTGAGTAGCGAAGAAGTGGTCGAGGAAATGATGTCTCATTGAGTATTCTCATGATAACGCCTCGATGCCGCTTTAGTAAGTTATCATAACTGCCACTGGTCCATCCACAAACGCCACTGAACGCAAGCTAGCTACAGACACGCTCATCACTATGACGTGTCTGTATATTAATAAGCTAACCCGCATTAAGTTAAc                                                                                                                                                                                                                      >  2:148819/1‑251 (MQ=255)
cacaGCAATCTTTATTAAATGAAATGGAAAACTGGCCAACGTATTATCCATATCAGTTGAGTAGCGAAGAAGTGGTCGAGGAAATGATGTCTCATTGAGTATTCTCATGATAACGCCTCGATGCCGCTTTAGTAAGTTATCATAACTGCCACTGGTCCATCCACAAACGCCACTGAACGCAAGCTAGCTACAGACACGCTCATCACTATGACGTGTCTGTATATTAATAAGCTAACCCGCATTAAGTTAAc                                                                                                                                                                                                                      >  2:166939/1‑251 (MQ=255)
cacaGCAATATTTATTAAATGAAATGGAAAACTGTCCAACTTATTATCCATATCAGTAGAGTAGCGAAGAAGTGGTCGAGGAAATGATGTCTCATTTAGTATTCTCATGATAACGCCTCGATGCCGTTTTAGTAATTTATCATAACTGCCCCTGGTCCATCCACAAACGCCACTGAACGCAATCTAGCTACAGACACGCTCATCACTATTACGTGTCTGTATATTAATAATCGAACCCGCATTAAGTTaa                                                                                                                                                                                                                       <  2:499581/250‑1 (MQ=255)
               taAATGAAATGGAAAACTGGCCGACGTATTATCCATATCAGTTGAGTAGCGAAGAAGTGGTCGAGGAAATGATGTCTCATTGAGTATTCTCATGATAACGCCGCGATGCCGCTTTAGTAAGTTATCATAACTGCCACTGGTCCATCCACAAACGCCACTGAACGCAAGCTAGCTACAGACACGCTCATCACTATGACGTGTCTGTATATTAATAAGCTAACCCGCATTAAGTTAACCAATAACGGATTcc                                                                                                                                                                                                        <  2:6408/250‑1 (MQ=255)
                                                                    gaagTGGTCGAGGAAATGATGTCTCATTGAGTATTCTCATGATAACGCCTCGATGCCGCTTTAGTAAGTTATCATAACTGCCACTGGTCCATCCACAAACGCCACTGAACGCAAGCTAGCTACAGACACGCTCATCACTATGACGTGTCTGTATATTAATAAGCTAACCCGCATTAAGTTAACCAATAACGGATTCCATACACAATTCGGCCAACAGAGAAAGATACCTGTGCTCACGCCATTGCTTatat                                                                                                                                                  <  1:470754/251‑1 (MQ=255)
                                                                                                gAGTATTCTCATGATAACGCCGCGATGCCGCTTTAGTAAGTTATCATAACTGCCACTGGTCCATCCACAAACGCCACTGAACGCAAGCTAGCTACAGACACGCTCATCACTATGACGTGTCTGTATATTAATAAGCTAACCCGCATTAAGTTAACCAATAACGGATTCCATACACAATACGGCCAACAGAGAAAGATACCTGTGCTCACGCCATTGCTGATTTTGGCTGGTTACAATATGCACTATCAAt                                                                                                                       <  1:257788/250‑1 (MQ=255)
                                                                                                         aaTGATAACGCCTCGATGCCGCTTTAGTAAGGTATCATAACTGCCACTGGGCCATCCACAAACGCCACTGAACGCAAGCTAGCTACAGACACGCTCATCACTATGACGTGTCTGTATATTAATAAGCTAACCCGCATTAAGTTAACCAATAACGGATTCCATACACAATACGGCCAACAGAGAAAGATACCTGTGCTCACGCCATTGCTTATATTGGCGGGTTACAATATGCACTAGCAATTTTTTAAATa                                                                                                             <  1:427941/250‑1 (MQ=255)
                                                                                                                 cGCCTCGATGCCGCTTTAGTAAGTTATCATAACTGCCACTGGTCCATCCACAAACGCCACTGAACGCAAGCTAGCTACAGACACGCTCATCACTATGACGTGTCTGTATATTAATAAGCTAACCCGCATTAAGTTAACCAATAACGGATTCCATACACAATACGGCCAACAGAGAAAGATACCTGTGCTCACGCCATTGCTTATATTGGCTGGTTACAATATGCACTATCAATTTTTTAAATAACAAatt                                                                                                      >  1:280406/1‑250 (MQ=255)
                                                                                                                            cGCTTTAGTAAGTTATCATAACTGCCACTGGTCCATCCACAAACGCCACTGAACGCAAGCTAGCTACAGACACGCTCATCACTATGAAGTGTCTGTATATTAATAAGCTAACCCGCATTAAGTTAACCAATAACGGATTCCATACACAATACGGCCAACAGAGAAAGATACCTGTGCTCACGCCATTGCTTATATTGGCTGGTTACAATATGCACTATCAATTTTTTAAATAACAAATTCTTAAAACcac                                                                                           >  1:322120/1‑250 (MQ=255)
                                                                                                                                ttAGTAAGTTAACATAACTGCCACTGGTCCATCCACAAACGCCACTGAACGCAAGCTAGCTACAGACACGCTCATCACTATGACGTGTCTGTATATTAATAAGCTAACCCGCATTAAGTTAACCAATAACGGATTCCATACACAATACGGCCAACAGAGAAAGATACCTGTGCTCACGCCATTGCTTATATTGGCTGGTTACAATATGCACTATCAATTTTTTAAATAACAAATTATTAAAACCACATCt                                                                                       <  2:373375/250‑1 (MQ=255)
                                                                                                                                            cATAACTGCCACTGGTCCATCCACAAACGCCACTGAACGCAAGCTAGCTACAGACACGCTCATCACTATGACGTGTCTGTATATTAATAAGCTAACCCGCATTAAGTTAACCAATAACGGATTCCATACACAATACGGCCAACAGAGAAAGATACCTGTGCTCACGCCATTGCTTATATTGGCTGGTTACAATATGCACTATCAATTTTTTAAATAACAAATTATTAAGACCACATCTGTATAATGAAtt                                                                           <  2:252642/250‑1 (MQ=255)
                                                                                                                                                         ggTCCATACACACACGCCCCTGACCGAAAGCATGCTACAGACCCGCTCATCACTATGAAGGGTCTGCATATTCATAAGCTCACCCGCCTTCAGTTAACCCATAACGGATTCCATACACAATACGGCCAACAGAGATAGATACCTGTGCTCACGCAATTGCTTATATTGGCTGGTTACAATATGCCCTATCACTTTTTTAAATAACAACTTATTAAAACCACATCTGTATACGGAATTTTTAAGGTTCGTgg                                                             >  2:190434/1‑251 (MQ=255)
                                                                                                                                                            ccatccaCAAACGCCACTGAACGCAAGCTAGCTACAGACACGCTCATCACTATGACGTGTCTGTATATTAATAAGCTAACCCGCATTAAGTTAACCAATAACGGATTCCATACACAATACGGCCAACAGAGAAAGATACCTGTGCTCACGCCATTGCTTATATTGGCTGGTTACAATATGAACTATCAATTTTTTAAATAACAAATTATTAAAACCACATCTGTATACGGAATTTTTAAGGTTCGTGGGAt                                                          >  1:280322/1‑251 (MQ=255)
                                                                                                                                                              atcctCAATCGCCACGGAACGCAAGCTAGCTACAGACACGCTAATCACTATGACGTGTCTGTATATTAAGAAGCGAACCCGCATTAAGTTAACCAATAACGGATTCTATACACAATACGGCCAACAGAGAAAGATACCTGTGCTCACGCCATTGCTTATATTGGCTGGTTACAATATGCTCTATCAATTTTTTAAATTACAAATTATTAAAACCTCAGCTGTAGAAGGAATTTTTAAGGTTCGTGTGATAg                                                        <  2:294080/251‑1 (MQ=255)
                                                                                                                                                                                                        catcaCTATGACGTGTCTGTATAATAATAAGCTAACCCGCATTAAGTTAACCAATAACGGATTCCATACACAATACGGCCAACAGAGAAAGATACCTGTGCTCACGCCATTGCTTATATTGGCTGGTTACAATATGCACTATCAATTTTTTAAATAACAAATTATTAAAACCACATCTGTATAAGGAATTTTTAAGGTTCGTGGGATAGCTTGACTGTGAAAATCACAGGAGCTACAAAAATGAACCGAt               <  2:16013/250‑1 (MQ=255)
                                                                                                                                                                                                                     tgtCTGTATATTAATAAGCTAACCCGCATTAAGTTAACCAATAACGGATTCCATACACAATACGGCCAACAGAGAAAGATACCTGTGCTCACGCCATTGCTTATATTGGCTGGTTACAATATGCACTATCAATTTTTTAAATAACAAATTATTAAAACCACATCTGTATAAGGAATTTTTAAGGTTCGTGGGATAGCTTGACTGTGAAAATCACAGGAGCTACAAAAATGAACCGATTCTCAAAAACTCa  >  1:1026/1‑250 (MQ=255)
                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                           
CACAGCAATCTTTATTAAATGAAATGGAAAACTGGCCAACGTATTATCCATATCAGTTGAGTAGCGAAGAAGTGGTCGAGGAAATGATGTCTCATTGAGTATTCTCATGATAACGCCTCGATGCCGCTTTAGTAAGTTATCATAACTGCCACTGGTCCATCCACAAACGCCACTGAACGCAAGCTAGCTACAGACACGCTCATCACTATGACGTGTCTGTATATTAATAAGCTAACCCGCATTGAGTTAACCAATAACGGATTCCATACACAATACGGCCAACAGAGAAAGATACCTGTGCTCACGCCATTGCTTATATTGGCTGGTTACAATATGCACTATCAATTTTTTAAATAACAAATTATTAAAACCACATCTGTATAAGGAATTTTTAAGGTTCGTGGGATAGCTTGACTGTGAAAATCACAGGAGCTACAAAAATGAACCGATTCTCAAAAACTCA  >  NC_000913/2041928‑2042390

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑