Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,652,331	T→C	intergenic (+2346/+397)	ydfD → / ← ynfP	lysis protein/protein YnfP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,652,331	0	T	C	100.0%	58.3 / NA	18	intergenic (+2346/+397)	ydfD/ynfP	lysis protein/protein YnfP
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (10/8);  total (10/8)

ATGGTGTGTTTCTGTTTATACTGGAGTTCGCCCTGGTGAGCTGTGTGCACTTGGATGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACTTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATGCCTGCTGGTCGCTGACAGCTGGTGCTAACCCGGCATTTATAGCAAACCAGATGG  >  NC_000913/1652087‑1652568                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                              actgggtggttcggattATACTGGAGTTCGCCCTGGTGAGCTGTGTGCACTTGGATGGGAGGACAGAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATATAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGAGTGAACCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCAGATCAATGATGGTCACCTCagag                                                                                                                                                                                                                                         <  2:411005/236‑1 (MQ=255)                                                  cTTGGATGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCCTATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTg                                                                                                                                                                                         >  1:237573/1‑250 (MQ=255)                                                    tGGATGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTAAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTAAGAAGGCAATAAAGGGACAGAGAAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAAAAAAAATGCACCTTTGTTTTTCAACCTGa                                                                                                                                                                                        >  1:482607/1‑249 (MQ=255)                                                                       cTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGTCATTCATCTTTTTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGCGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAAAGCACCCTTGTTTTTTAACCTGAAGTGTCAGCGAGAGTaaaaa                                                                                                                                                                    >  1:47787/1‑250 (MQ=255)                                                                                           gatCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAAACAAACGGGACATTCATCTTATTAAGCCAGCAAGCCGCGCTCTCCGGCGGCAGATGACCATAACGAGACTGAGCAAAAAGCATATCATTGATGTTTAACTCATAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAAGGTCAGGGAGAGGAAAAAATTTAGGGGAACATTTTACCg                                                                                                                                               >  2:274558/1‑251 (MQ=255)                                                                                               aGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGACATTCATCTTATTAAGCCCGCAATCGACGCCCTCCGGGGTCAGATGACATTAACGAGACTGAGCACAGAGCATATCATTGATGTTCACCCCAGAGAGTATGGCAGAAAAGAAAAACAAAAATGCACCCTTGTTTTTCAAACTGAAGTTTCAGCGAGAGTAAAAAAATATGGTGACCCTTTTACCGTTgg                                                                                                                                           >  1:269406/1‑250 (MQ=255)                                                                                                                     gTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGAc                                                                                                                     <  2:237573/251‑1 (MQ=255)                                                                                                                     gTTTCACGGTACCAAAAACACAGGCGGGAACCAATAGGGGCATTCATCCTTTTAAGCCAGCAATCGACGCCCTCCGGAGTCAGATGACATTAACGAGACTGAGCACAGAGCAAAAAATTGATGTTCACCTCAGAGAGAATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTTAAGTGTCAGCGAGAGTAAAAAAATATGGTGACCATTTTAACGTTGACTCAATAAGGCCGGTGTGGGAc                                                                                                                     >  1:140183/1‑251 (MQ=255)                                                                                                                       ttCACGGTACCAAAAACACAGGCGGGAACCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACg                                                                                                                    <  1:268393/250‑1 (MQ=255)                                                                                                                                         caGGCGGGAACCAATCGGGACATTCATCTTATTAAGCCAGCAATCGACGATCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGAATCAATAAGGCAGATGTGGGACGCAGAGATAAAACGTGCCgg                                                                                                 >  2:249050/1‑251 (MQ=255)                                                                                                                                            gCGGGAACCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACt                                                                                              <  1:132888/251‑1 (MQ=255)                                                                                                                                                                                                                aaCGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATGCCTGCTGGTCGCTGACAGCTGGTGCTAAcc                          >  1:344122/1‑251 (MQ=255)                                                                                                                                                                                                                     gaCTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTTCCGGACTCCGCCATCGAAAATCATATCAGTCGATACATACTTATGCCTGCTTGTCGCTGACAGCTGGTGCTAACCCGGCa                     <  2:481756/251‑1 (MQ=255)                                                                                                                                                                                                                      aCTGAGCAAAGAGCAGATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTGTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGTGACGCAGCGATAAAACTTGCCGGACGCCGCCATCGAAAATCCTATCAGTCGAGACATACTTAGGCCTGCTGGTCGCTGGCAGCTGGTGCTAACCCGGCAt                    <  2:482607/251‑1 (MQ=255)                                                                                                                                                                                                                                   cATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCa                                                                                                                  >  2:186002/1‑144 (MQ=255)                                                                                                                                                                                                                                   cATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCa                                                                                                                  <  1:186002/144‑1 (MQ=255)                                                                                                                                                                                                                                              tGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTCCCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATGCCTGCTGGTCGCTGACAGCTGGTGCTAACCCGGCATTTATAGCAAACCAGATgg  >  2:455247/1‑245 (MQ=255)                                                                                                                                                                                                                                              tGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATGCCTGCTGGTCGCTGACAGCTGGTGCTAACCCGGCATTTATAGCAAACCAGATgg  <  1:455247/245‑1 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                              ATGGTGTGTTTCTGTTTATACTGGAGTTCGCCCTGGTGAGCTGTGTGCACTTGGATGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACTTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATGCCTGCTGGTCGCTGACAGCTGGTGCTAACCCGGCATTTATAGCAAACCAGATGG  >  NC_000913/1652087‑1652568

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑