Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,358,859	T→C	Y110C (TAT→TGT)	puuP ←	putrescine:H(+) symporter PuuP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,358,859	0	T	C	100.0%	48.4 / NA	17	Y110C (TAT→TGT)	puuP	putrescine:H(+) symporter PuuP
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (6/11);  total (6/11)

GACGGTGCCAACGCCTTCTCCTTTATGCAGTCCCTGAACTACCAGGAAGATAAACACCACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGATAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGACAATACCAAAAGTATCAAATACGGTCATCGGCGT  >  NC_000913/1358615‑1359082                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                gACGGTGCCAACGCCTTCTCCTTTATGCAGTCCCTGAACTACCAGGAAGATAAACACCACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCca                                                                                                                                                                                                                           <  1:266091/251‑1 (MQ=255)     gggCCAACTCCCTCTCCATTAAGCAGACCCCGAACTACCAGTAAGTTAAACACCACCATGAAGGAGATGTGCACCAGTTCAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGAGAGAGTTTCGCCAACAAGACGTGAATCATCGGCAAAAATAGACAATCcagcag                                                                                                                                                                                                                       <  2:62348/249‑1 (MQ=255)             gCCTTCTCCTTTATGCAGTCCCTGAACTACCAGGAAGATAAACACCACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGAcc                                                                                                                                                                                                               <  1:206305/251‑1 (MQ=255)              ccTTCTCCTTTATGCAGTCCCTGAACTACCAGGAAGATAAACACCACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCa                                                                                                                                                                                                              <  1:95144/251‑1 (MQ=255)                             aGTCCCTGAACTACCAGGAAGATAAACACCACCATGATGGAGAGTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATcc                                                                                                                                                                                               <  1:352196/251‑1 (MQ=255)                                                                           gCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAAcc                                                                                                                                                  <  2:392291/250‑1 (MQ=255)                                                                                    cAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCCCACCCACGGCGGCACTTCCGGGAAGAGGGCGGGGGGATAGATTTTTGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGAAGACCAGCAGACCCTAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGGAGGTATAGGGCGAAACGGCCTCCggg                                                                                                                                        >  2:11320/1‑250 (MQ=255)                                                                                                 gAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAAGCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGTAAACTGGCGAACCa                                                                                                                           <  2:180907/251‑1 (MQ=255)                                                                                                 gAAGGTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAACATGGCGACGAAGGTTACCACCCCCACCCACGGCGGCACCTCCGGGAAGAGGGCGGAGAGATAGATTTTTGCCAACAAGACGCTAATAATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATACGACGTGAGGGGTAATCGACTTTTGCGCGTAGGTATAGGCCGAACACGCCTCCGGAAAATGGCGAAcc                                                                                                                            >  1:299192/1‑250 (MQ=255)                                                                                                                         ctTCAGATTCGCGGCGGGTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGTAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCAGCGGCAAAAAGAGACAATCCAGCAGCGATGACCATCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTTCGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGt                                                                                                     <  1:407998/249‑1 (MQ=255)                                                                                                                                                                           ccggcggCACGTCCGGAAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTGAATCATCGGAAAAAAGAGACAATCCAGCAGCGATTACCAGCCGACCATAAATCCGTCGTGCGGGTTAAGCTTTTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGTAAACTGGCGATCCATTTTGCCGTAGCTGATAGCGTTAAACGGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCg                                                 <  1:293206/250‑1 (MQ=255)                                                                                                                                                                                      cccGGGAAAAGGGCGGAGAGATAGATTTCCGCCAACAGAACTTTAATCCTCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAACCCGACGTGCGGGTTAATCGACTTTGGCGGGTGGGTATAGGCCTAACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACGGCACCCCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGga                                      <  2:152968/250‑1 (MQ=255)                                                                                                                                                                                                    ggAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCaa                                                                          >  1:357848/1‑201 (MQ=255)                                                                                                                                                                                                      agagaTAGATTTTCGCCAACAAGACGTTAATCATCGGCAAACAGACCCACTCCAGCAGCGATGACCAGCCGACCCTACCTCCGACGTGCGGGTCAATCGACTCTTGCGCGTAGGTATAGGCCGAACCCGCCTCCGAAAACTGGCGCCCCAGTTTGCCGTCGACGCTAGCGGCAAACAGCACCCCCGACAGCGCCAGCAAATAGGACGCCGGAACGAGACCGAAGCTAATGCCGGCGACACTACCAAAAgcc                      >  1:214576/1‑249 (MQ=255)                                                                                                                                                                                                                       aacaaGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTGGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGACAATACCAAAAGTATCAAATACGGTCATCgg     <  1:142872/251‑1 (MQ=255)                                                                                                                                                                                                                         caaGACGTTAATCATCGGCAAAAAGAGACACTCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGACAATACCAAAAGTATCAAATACGGTCATCGGCg   >  1:382899/1‑251 (MQ=255)                                                                                                                                                                                                                          aaGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGACAATACCAAAAGTATCAAATACGGTCATCGGCGt  >  2:168953/1‑251 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                GACGGTGCCAACGCCTTCTCCTTTATGCAGTCCCTGAACTACCAGGAAGATAAACACCACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGATAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGACAATACCAAAAGTATCAAATACGGTCATCGGCGT  >  NC_000913/1358615‑1359082

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑