Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,301,992 A→T N271Y (AAT→TAT)  oppA → oligopeptide ABC transporter periplasmic binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,301,9920AT96.7% 94.4 / NA 30N271Y (AAT→TAT) oppAoligopeptide ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base T (17/12);  total (18/12)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

CACCGGTGCCAAAAGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTAT  >  NC_000913/1301753‑1302204
                                                                                                                                                                                                                                               |                                                                                                                                                                                                                    
cacggtctccAAAAGCCTCTAACGTGAAATTCTGCGAAAAATAGCCCCAGCCTGGTAATATCGTCACCACCGGTTCCTATTCCTTAAAAGATTGGGTCTTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCTTTATTATCCAGGTATCCTATTTGCCTATAGTTTCTGAAGGAAACTATGTCAACCGCTACCGTAGTGGTGAAATCGTCATGACTTATAACATCATg                                                                                                                                                                                                           <  2:311376/243‑1 (MQ=255)
cACCGGTGCCAAAAGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATAGTTCTTGAACGCAGCCCGACATACTGGAACAACGCGAAAACCATTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGACGTTACCGATGTCAACCGCTACCGTAGAGGTGAAATCGACATGACGTATAACAGCATg                                                                                                                                                                                                           >  1:281531/1‑251 (MQ=255)
   cGGTGCCAAAAGCCGCTATCGAGAAAGTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCAGGCCg                                                                                                                                                                                                        <  2:340646/251‑1 (MQ=255)
             aGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAATATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTAGAACAGCATGCCGAGCGAATTGt                                                                                                                                                                                              <  1:347014/251‑1 (MQ=255)
                  cTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGCATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTcc                                                                                                                                                                                           >  2:490060/1‑249 (MQ=255)
                     acGAGAAAATCGGCGAAAAATGGACCCAGGCTGGGTATATCCTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAg                                                                                                                                                                                      <  1:99525/250‑1 (MQ=255)
                          aaaTTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGAAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGa                                                                                                                                                                                  >  1:12064/1‑250 (MQ=255)
                                    aaaaaTGGCGCCATCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAATATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATGTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTTCCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATc                                                                                                                                                                       <  1:445177/251‑1 (MQ=255)
                                        aTGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCgg                                                                                                                                                                   <  1:246493/251‑1 (MQ=255)
                                                    tGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGCAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACg                                                                                                                                                       >  1:112658/1‑251 (MQ=255)
                                                    tGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGAGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACg                                                                                                                                                       >  1:203627/1‑251 (MQ=255)
                                                        aaTATCGTAAACCACGATGCCTAAACCTTAAAGGATTGGGTCGTAAACGCACGCATCGTTCTTGTACGCACCCCGCGCTACTGGAACAACGCGCAAACCGTTCTTAACCAGGTAACCTATGTGCATATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGCGGTGAAATCGACATGACGTATAACAGCATGCCAATCGAATTGTTCCAGAAGCTGAAACAGGAAATCCCTGACGTAGTTCACGtttg                                                                                                                                                   >  2:147706/1‑249 (MQ=255)
                                                         atatCGTAGCCAACGGTGCCTATACCTTAAAAGATTGGTTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTCCTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTACCTATTGCTTCTGAAGTTACAGATGTCAACCGCGACCGTAGTGGTGCAATCGACATGACGTATAACAGCAAGCCGATCGAATTGTTCCAGAAGCTGAAAAACGAGATACCGGAAGAAGATCACGtttct                                                                                                                                                  >  2:271281/1‑248 (MQ=255)
                                                                cACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGAGCCCGGACGAAGTTCACGTTGATCCATAc                                                                                                                                            <  2:273014/250‑1 (MQ=255)
                                                                 aCCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCt                                                                                                                                          <  1:490060/251‑1 (MQ=255)
                                                                   cAACGGGGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTGGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCtgt                                                                                                                                        <  2:281552/251‑1 (MQ=255)
                                                                                              ggTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACATACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCACCCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGCATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGCTCCATACCTGTGCACTTACTATTACGAAATTAACAAcc                                                                                                             >  2:289285/1‑251 (MQ=255)
                                                                                                            gaaTCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCAtt                                                                                                >  2:281993/1‑250 (MQ=255)
                                                                                                                    cTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGAtgt                                                                                       >  2:35578/1‑251 (MQ=255)
                                                                                                                    cTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGAtgt                                                                                       >  1:403490/1‑251 (MQ=255)
                                                                                                                                      tACTGGAACAACGAGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGAGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACt                                                                     >  2:203325/1‑251 (MQ=255)
                                                                                                                                          ggAACAACGCGAAAAACGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGCCGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTACAAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGaa                                                                  >  2:407211/1‑250 (MQ=255)
                                                                                                                                            aacaacGCGAAAACAGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGACTTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCATTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGAGTGTGCTTACCGCACTGAAACt                                                               >  2:145017/1‑251 (MQ=255)
                                                                                                                                                                           ccTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTtaataa                                 >  1:323908/1‑250 (MQ=255)
                                                                                                                                                                                   ccTATTGCTTCTGAAGTTACCGATGTCAACCGCTCCCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAgc                        <  2:403490/251‑1 (MQ=255)
                                                                                                                                                                                        tGCTTCTGACGATCCCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCAAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAgg                   >  2:335975/1‑251 (MQ=255)
                                                                                                                                                                                                aaGTTACCGATGTCAACCGCTACCTTAGTGTTTAAATCTACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGTTCCCGGACGAAGTTCCCGTTGATCCATACCTTTTCACTTACTATTACGAAATTACCAACCATAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGCCCGCGATATCATTGTTAATAAAGTGAAATCGCAGGGCAACATg           <  2:226169/251‑1 (MQ=255)
                                                                                                                                                                                                     cccGATGTCAACCGCTACCTTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACAAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGc      <  1:48651/250‑1 (MQ=255)
                                                                                                                                                                                                       cGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATA‑CAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCAGGACGAAGTTCACGTTGATCAATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCt    >  1:287681/1‑250 (MQ=255)
                                                                                                                                                                                                         aTGTCAACCGCTACCGTAGTGGTGAACTCGCCATGCCGTATAACATCCTGCCGCTCGACTTGTTCCAGAAGCTGCAACAAGAGCTCCCGGACGAAGTTCACGTTGATCCATACCTGTGCCCTTACTCTTACGCAATTAACAACCAGACACCGCCATTCAACGATGTTCGTGTGCGTACCGCACTGACACTAGGTATGGACCGCGATATCATTGTTAATAACTTGAAAACGCAGTGCAACCTGCCCGCCTAt  >  2:72440/1‑251 (MQ=255)
                                                                                                                                                                                                                                               |                                                                                                                                                                                                                    
CACCGGTGCCAAAAGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTAT  >  NC_000913/1301753‑1302204

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑