Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	246,747	G→T	S12S (TCG→TCT)	yafL →	NlpC/P60 family protein YafL

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	246,747	0	G	T	100.0%	164.0 / NA	50	S12S (TCG→TCT)	yafL	NlpC/P60 family protein YafL
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (30/20);  total (30/20)

GGGTATCTCCTGTGCTGTATATTTATTCAGCTTGAATTGTAGCTCAAATGAGCAACAGCGACAATCCATGAGTAATTTAATAAACGGATGTTTTTACATAATTAACTGAAATATATATGTTAATTTTATAATAATGGTTTATTAAGTTTGTTCAGAAACGATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCGGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCCAGCTATATTGTGGAAGGCAATGCCGAAAGCAAAAGGGCGCTACGCCAGCACAACCGGGAGCAGATAAAACAGCATCCAGAATGGTTTCCTGCTCCGCTCA  >  NC_000913/246505‑246991                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                     gggTATCTCCTGTGCTGTATATTTATTCAGCTTGAATTGTATCTCAAATGATCAACAGCGACAATCCATGAGTAATTTAATAAACGGATGTTTTTACATAATTAACTGAAATATATATGTTAATTTTATAATAAGTGTTTATTAAGTTTGTGCAGAAACGATCCGGGATACACTGCAACATTAAAGCATGAGCAGCCATTATTGAGTATGTGCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACt                                                                                                                                                                                                                                              <  2:36971/251‑1 (MQ=255)   gTATCTCCTGTGCTGTATATTTATTCAGCTTGAATTGTAGCTCAAATGAGCAACAGCGACAATCCATGAGTCATTTAATAAACGGATGTTTTTACATAATTAACTGAAATATATATGTTAATTTTATAATAATGGTTTATTAAGTTTGTTCAGAAACGATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGt                                                                                                                                                                                                                                            >  1:26551/1‑251 (MQ=255)                 gTATATTTATTCAGCTTGAATTGTAGCTCAAATGAGCAACAGCGACAATCCATGAGTAATTTAATAAACGGATGTTTTTACATAATTAACTGAAATATATATGTTAATTTTATAATAATGGTTTATTAAGTTTGTTCAGAAACGATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCg                                                                                                                                                                                                                              >  1:426676/1‑251 (MQ=255)                   ataattattCAGCTTGAATTGTAGCTCAAATGAGCAACAGCGACAATCCATGAGTAATTTAATAAACTGATGTTTTTACATAATTAACTGAAATATATATGTTAATTTTATAATAATGGTTTATTAAGTTTGTTCAGAAACGATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTAGTTAGTATGTCCTTGCCGTCGATTCCATCCTGTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGtt                                                                                                                                                                                                                            <  2:161878/247‑1 (MQ=255)                     atttattCAGCTTGAATTGTAGCTCAAATGAGCAACAGCGACAATCCATGAGTAATTTAATAAACGGATGTTTTTACATAATTAACTGAAATATATATGTTAATTTTATAATAATGGTTTATTAAGTTTGTTCAGAAACGATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGttt                                                                                                                                                                                                                           <  1:2366/250‑1 (MQ=255)                            aagtttGAATTGTAGCTCAATTGGACATCATCGGCAATCCATGAGTAATTTAATAAACGAATGTTTTTATATAATTAACTGAAGTATATAAGTTAATGTTATAATAATTGTTTATTTAGTTTGTCCAGAAACTAACGGGGGTTCACTGCAACATTTAAGCATGCCCAGCCATTATTAAGTATGTCCTAGCCGTCGATTCCAGCCTGTGGATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCAt                                                                                                                                                                                                                    <  2:446425/246‑1 (MQ=255)                                        tAGCTCAAATGATCCACCGCGACAATACATGAGAACTTTAATAAACCGATGTTTTTACCTACTTAACTGAAATATATATGTTAATTTTATAATAATGGTTTATTAAGTTTGTTCAGACACGATCCGGGATACACTGCAACATTAAAGCATGACAAGCCATTAGTGAGAATGTCCTTGCCGTAGATTACATCCTTTGTATTGTCTGGATTAATGTTTATTTTTTTGCCGTTTTCTTCATTTGCCAGCGccac                                                                                                                                                                                                       >  2:444499/1‑251 (MQ=255)                                             cAAATGAGCAACAGCGACAATCCATGAGTAATTTAATAAACGGATGTTTTTACATAATTAACTGAAATATATATGTTAATTTTATAATAATGGTTTATTAAGTTTGTTCAGAAACGATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCcaccac                                                                                                                                                                                                    <  1:90205/249‑1 (MQ=255)                                              aaaTGAGCAACAGCGACAATCCATGCGTAATTTAATAAACGGATGTTTTTACATAATTAACTGAAATATATATGTTAATTTTATAATAATGGTTTATTAAGTTTGTTCAGAAACGATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACcacatc                                                                                                                                                                                                 >  2:235020/1‑251 (MQ=255)                                                      aaCAGCGACAATCAAGGAGTAATTTAATAAACGGAGGTTTTTACATAATTAACTGAAATATATATGTTAATTTTATAATAATGGTTTATTAAGTTTGTTCAGAAACGATCCGGGATACGCTGCAACATTAAAGCAGGGCCAGCCATTATTTAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATGGTCTGGATTACTGTTGATTTTTTTGCCGTTTTCGTCATTTGCCAGCGCCACCACAGCACATAtct                                                                                                                                                                                         <  2:27178/251‑1 (MQ=255)                                                         aGCGACAATCCATGAGTAATTTAAGAAACGGATGTTTTTACATAATTAACTGAAATATATATGTTAATTTTATAATAATGGTTTATTAAGTTTGTTCAGAAACGATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTGCTTCATTTGCCAGCGCCACCACATCACATATCTCtt                                                                                                                                                                                      <  2:269941/251‑1 (MQ=255)                                                            gACAATCCATGAGTAATTTAATAAACGGATGTTTTTACATAATTAACTGAAATATATATGTTAATTTTATAATAATGGTTTATTAAGTTTGTTCAGAAACGATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTc                                                                                                                                                                                    >  1:407247/1‑250 (MQ=255)                                                                             ttAATAAACGGATGTTTTTACATAATTAACTGAAATATATATGTTAATTTTATAATAATGGTTTATTAAGTTTGTTCAGAAACGATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCcgcccgcc                                                                                                                                                                    >  1:113613/1‑249 (MQ=255)                                                                                   aaCGGATGTTTTTACATAATTAACTGAAATATATATGTTAATTTTATAATAATGGTTTATTAAGTTTGTTCAGAAACGATCAGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGGGTATGTACTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATTTCTCTTTCAGCTACTCCGCCCGCCCGCggctg                                                                                                                                                            >  2:101133/1‑248 (MQ=255)                                                                                    aCGGATGTTATTACATAATTAACCGAACTATATATGTTACTTTTATACTAATGGTTTATTAAGTTTGTTCATAAACGATCCGTGATACACTGCAACATTAAAACATGCACAGCCATCATTGAGTATGGCCTTGCCGTCGATTCCATCATTTGTCTTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACTACATCCCTTATATCTTTACGCTACGCCGCACGCCAGCGGATGc                                                                                                                                                           >  2:35771/1‑251 (MQ=255)                                                                                            ttttACATAATTAACTGAAATATATATGTTAATTTTATAATAATGGTTTATTAAGTTTGTTCAGAAACGATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGt                                                                                                                                                   >  1:164697/1‑251 (MQ=255)                                                                                                  aTAATTAACTGAAATCTATATGTTAATTTTATAATAATGGTTTATTAAGTTTGTTCAGAAACGATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGt                                                                                                                                             >  1:423148/1‑251 (MQ=255)                                                                                                  aTAATTAACTGAAATATTTTGGTTAATTTTTAAATAATGGTTTATTAAGTTTTTTACGAACCGATCCGGGTTACACTGAAACATTAAATAATGCCCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGTATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACAACACATTCCTCTGTCAGCTGCGCCGCCCGCCAGCGGATGCATAACCGTGCGCGt                                                                                                                                             <  2:314575/251‑1 (MQ=255)                                                                                                   aaaTTAACGGAAATATTTATGTTAATTTTATAATAATGGTTTATTAATTTTTTTCAGAAACGATCCGGTATCCACTCCAACATTAAAGCATGCCCAGCCTTTATTGAGTATTTCCTGTCCGTCGATTCCATCCTTTGTATTTTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTTCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGcgcg                                                                                                                                              <  2:387445/248‑1 (MQ=255)                                                                                                        aaCTGAAATATATATGTTAATTTTATAATAATGGTTTATTAAGTTTGTTCAGAAACGATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTTTATTGTCTGGATTACTGTTGATTTGTTTGCCTTTTTCTTCATTTGCCACCGCCACCACATCACATAGCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTttatt                                                                                                                                        <  1:326347/250‑1 (MQ=255)                                                                                                              aaTATATATGTTAATTTTATAATAATGGTTTATTAAGTTTGTTCAGAAACGATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTTCTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTGCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAg                                                                                                                                 <  1:497912/251‑1 (MQ=255)                                                                                                                      tGTTAATTTTATAATAATGGTTTATTAAGTTTGTTCAGAAACGATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTCTTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAAc                                                                                                                         >  1:286350/1‑251 (MQ=255)                                                                                                                          aaTTTTATAATAATGGTTTATTAAGTTTGTTCAGAAACGATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCTACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCAt                                                                                                                     >  1:62016/1‑251 (MQ=255)                                                                                                                          aaTTTTATAATAATGGTTTATTAAGTTTGTTCAGAAACGATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCAt                                                                                                                     >  2:271941/1‑251 (MQ=255)                                                                                                                                ataataATGGTTTATTAAGTTTGTTCAGAAACGATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACAAACCTCATCTGaa                                                                                                                >  2:354477/1‑250 (MQ=255)                                                                                                                                   ataatGGTTTATTAAGTTTGTTCAGAAACGATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCAt                                                                                                                     >  2:8374/1‑242 (MQ=255)                                                                                                                                   ataatGGTTTATTAAGTTTGTTCAGAAACGATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCAt                                                                                                                     <  1:8374/242‑1 (MQ=255)                                                                                                                                        ggTTTATTAAGTTTGTTCAGAAACGATCCGGGATACACTGCAACATTAAAGAATGACCAGACATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACTCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGc                                                                                                       >  2:307826/1‑251 (MQ=255)                                                                                                                                                      gttcaaaacctaTCCGGGATACACTGCAAAATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTTTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCCAGCTATATTGTgg                                                                                         <  2:446672/240‑1 (MQ=255)                                                                                                                                                      gTTCAGAACCGATCCGGGATAAACTGCAACATTAAAGCATGACCAGCCATCATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTATGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGACAAAGCAGGCAAGCTATATTGTgg                                                                                         >  2:339850/1‑251 (MQ=255)                                                                                                                                                         caaaaaCGATCGGGTATCCCCTGAAACATTAAAGCATGCCCAGCCATTATTGAGTATGTCCTGGCCGTCGATTCCATCCTTTGTATTTTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCCAGCtatagaggggaag                                                                                      <  2:286350/248‑10 (MQ=255)                                                                                                                                                         cAGAAACGATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGCCGATTCCATCCTTTGTAGTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATAGCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCCAGCTATATTGTGGAAg                                                                                      <  1:323912/251‑1 (MQ=255)                                                                                                                                                               cGATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCCAGCTATATTGTGGAAGGCAATg                                                                                >  2:205541/1‑251 (MQ=255)                                                                                                                                                                  tCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCCAGCTATATTGTGGAAGGCAATGCCg                                                                             >  1:179770/1‑251 (MQ=255)                                                                                                                                                                             cTGCAACATTAAAGCATGACCAGCCATTATTGAGAATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATCTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCACGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACAAAACTCATCTGAAAAAGCAGGCCAGCTATATTGTGGAATGCAATGCCGAAAGAAAAAgg                                                                  >  1:302751/1‑251 (MQ=255)                                                                                                                                                                                cAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGCTTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGAGACACCACATCACATATCTCTTTCAGCTACCCCGCCCGCCAGCGGCTGCAAAACCGTGCGCGTTTATTAAAACAGTACCAACCTCATCTGAAAAAGCAGGCCAGCTATATTGTGGAAGGCAATGCCGAAAGCCAAAGGgcg                                                               >  1:257509/1‑251 (MQ=255)                                                                                                                                                                                cAACATTAAAGCAGGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCTCATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCCAGCTATATTGTGGAAGGCAATGCCGAAAGCAAAAGGgcg                                                               <  1:474519/251‑1 (MQ=255)                                                                                                                                                                                 aaCATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCCAGCTATATTGTGGAAGGCAATGCCGAAAGCAAAAGGgcgc                                                              >  1:377741/1‑251 (MQ=255)                                                                                                                                                                                   cATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCCAGCTATATTGTGGAAGGCAATGCCGAAAGCAAAAGGGCGCt                                                             >  2:118275/1‑250 (MQ=255)                                                                                                                                                                                        aaGCATGACCAGCCATTATTGAGGATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCCAGCTATATTGTGGAAGGCAATGCCGAAAGCAAAAGGGCGCTACGcc                                                        >  2:111695/1‑250 (MQ=255)                                                                                                                                                                                                 cAGCCATTATTGATTATGGCCTTGCCGTCGATTCCATCCTTTGGATTGTCTGGATTACTGTTGATTTGTTTGCCTTTTTCTTCATTTGCCAGCGCCCCCACATCACATATCGCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCCAGCTATATTGTGGAAGGCAATGCCGAAAGCAAAAGGGCGCTACGCCAGCACAACCg                                              <  1:307826/251‑1 (MQ=255)                                                                                                                                                                                                 cAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCCAGCTATATTGTGGAAGGCAATGCCGAAAGCAAAAGGGCGCTACGCCAGCACAACCg                                              <  1:205541/251‑1 (MQ=255)                                                                                                                                                                                                     aattattGAGGATGTCCTTGCCGTCAGTTCCAACTTGTTTATTGTGTGGTTTACTATTGAGTTTTTTGCCGTGTTCTTCATTTGTCAGCGCCGCCATGTCACATAGCTCGGTCGGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTGATTAAAACAGTACCAAACTCATCGGAGAAAGCAGGCCAGCTATGTTGTTGAAGGCAATTACGGAAGCAAATTGGCGCTTGGCCAGCACAACCGGGAg                                          <  2:470768/250‑1 (MQ=255)                                                                                                                                                                                                           tGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAAAAGTACCAAACTCATCTGAAAAAGCAGGCCAGCTATATTGTGGAAGGCAATGCCGAAAGCAAAAAGGCGCTAAGCCAGCACAACAGGGAGAAGAt                                     >  1:284680/1‑250 (MQ=255)                                                                                                                                                                                                            gAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCCAGCTATATTGTGGAAGGCAATGCCGAAAGCAAAAGGGCGCTACGCCAGCACAACCGGGAGCAGATa                                    >  2:364563/1‑250 (MQ=255)                                                                                                                                                                                                                                ttCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCCAGCTATATTGTGGAAGGCAATGCCGAAAGCAAAAGGGCGCTACGCCAGCACAACCGGGAGCAGATAAAACAGCATCCAGAATGGttt               >  2:11614/1‑251 (MQ=255)                                                                                                                                                                                                                                  ccATCCCTTGAATTGTCTGGATTGCTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCCAGCTATATTGTGGAAGGCAATGCCGCAAGCAAAAGGGCGCTACGCCAGCACAACCGGGAGCAGATAAAACAGCATCCAGAATGGTTTcc             >  2:81214/1‑251 (MQ=255)                                                                                                                                                                                                                                    aTCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCCAGCTATATTGTGGAAGGCAATGCCGAAAGCAAAAGGGCGCTACGCCAGCACAACCGGGAGCAGATAAAACAGCATCCAGCATGGTTTCCTg           >  1:209369/1‑251 (MQ=255)                                                                                                                                                                                                                                            tATTGTCTGGATTCCGGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCCAGCTATATTGTGGAAGGCAATGCCGAAAGCAAAAGGGCGCTACGCCAGCACAACCGGGAGCAGATAAAACAGCATCCAGAATGGTTTCCTGCTCCGCt    <  2:148687/250‑1 (MQ=255)                                                                                                                                                                                                                                               tGTCTGGATTACTGTTGATTTGTTTGCCGTCTTCTTCCTTTGCCAGCGCCACCACATCACATATCTCTTTCAGTTACACAGCCCGCCAGCGGCTGCCAAACCGTGCGCGTTTATTAACACAGTACAAAACTCATCTGAAAAAGCAGGACCGCTATATTGTGGAAGGCAATGCCGAAAGCAAAAGAGCGCCACGCAAGCACAACAGGGAGAAGATAAACCAGCATCCAGACTGGTTTCCTGCTCCCCTCa  >  2:220851/1‑249 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                     GGGTATCTCCTGTGCTGTATATTTATTCAGCTTGAATTGTAGCTCAAATGAGCAACAGCGACAATCCATGAGTAATTTAATAAACGGATGTTTTTACATAATTAACTGAAATATATATGTTAATTTTATAATAATGGTTTATTAAGTTTGTTCAGAAACGATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCGGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCCAGCTATATTGTGGAAGGCAATGCCGAAAGCAAAAGGGCGCTACGCCAGCACAACCGGGAGCAGATAAAACAGCATCCAGAATGGTTTCCTGCTCCGCTCA  >  NC_000913/246505‑246991

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑