Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 557,144 C→A V200L (GTA→TTA)  folD ← bifunctional methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913557,1440CA98.6% 231.1 / NA 69V200L (GTA→TTA) folDbifunctional methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base A (37/31);  total (38/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01

AACGCACGCCTGTAGCGTGTTTTCAATCAGCGTGGCAACCGTCATCGGGCCAACGCCGCCGGGAACAGGCGTAATGTATGAGGCGCGTTTAGCCGCGTCTTCAAACACGACGTCGCCCACAACTTTGCCATTTTCCAGACGGTTGATGCCGACATCAATCACAATTGCGCCTTCTTTGATCCAGTCACCGGGAATAAAGCCTGGCTTGCCAACGGCAACGATCAATAGATCGGCATTTTCTACGTGATGACGCAGATTTTTAGTGAAGCGGTGAGTCACTGTAGTGGTGCAACCTGCCAGCAGCAGTTCCATGCTCATCGGGCGGCCAACGATATTCGATGCGCCAATCACCACGGCGTTGAGGCCGAAGGTATCAATGTTGTAACGCTCAAGCAGCGTGACGATACCGCGCGGGGTGCAGGGACGCAGACGCGGCGCGCGCTGGCACAGACGACCGACGTTGTAAGGATGGAAACCGTCCAC  >  NC_000913/556902‑557384
                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                
aaCGCACGCCTGTAGCGTGTTTTCAATCAGCGTGGCAACCGTCATCGGGCCAACGCCGCCGGGAACAGGCGTAATGTATGAGGCGCGTTTAGCCGCGTCTTCAAACACGACGTCGCCCACAACTTTGCCATTTTCCAGACGGTTGATGCCGACATCAATCACAATTGCGCCTTCTTTGATCCAGTCACCGGGAATAAAGCCTGGCTTGCCAACGGCAACGACCAATAGATCGGCATTTTCTAAGTGATGAc                                                                                                                                                                                                                                          >  1:480356/1‑251 (MQ=255)
 acgcacgcCTGTAGCGTGTTTTCAATCAGCGTGGCAACCGTCATCGGGCCAACGCCGCCGGGAACAGGCGTAATGTATGAGGCGCGTTTAGCCGCGTCTTCAAACACGACGTCGCCCACAACTTTGCCATTTTCCAGACGGTTGATGCCGACATCAATCACAATTGCGCCTTCTTTGATCCATTCACCGGGACTAAACCCTGGCTTACCAACGGCCACGATCAATAGATCGGCATTTTCTAAGTGATGACg                                                                                                                                                                                                                                         >  1:179/1‑251 (MQ=255)
  cgcacgcCTGTAGCGTGTTTTCAATCAGCGTGGCGTCCGTCATCGGGCCAACGCCGCCGGGAACAGGCGTAATGTATGAGGCGCGTTTAGCCGCGTCTTCAAACACGACGTCGCCCACAACTTTGCCATTTTCCAGACGGTTGATGCCGACATCAATCACAATTGCGCCTTCTTTGATCCAGTCACCGGGAATAAAGCCTGGCTTGCCAACGGCAACGATCAATAGATCTGCATTTTCTAAGTGATGACGc                                                                                                                                                                                                                                        <  2:436186/251‑1 (MQ=255)
     acgcCTGTAGCGTGTTTTCAATCAGCGTGGCAACCGTCATCGGGCCAACGCCGACGGGAACAGGCGTAATGTATGAGGCGCGTTTAGCCGCGTCTTCAAACACGACGTCGCCCACAACTTTGCCATTTTCCAGACGGTTGATGCCGACATCAATCACAATTGCGCCTTCTTTGATCCAGTCACCGGGAATAAATCCTGGCTTGCCAACGGCAACGATCAATAGATCGGCATTTTCTAAGTGATGACGc                                                                                                                                                                                                                                        >  2:222786/1‑248 (MQ=255)
      cgcCTGTAGCGTGTTTTCAATCAGCGTGGCAACCGTCATCGGGCCAACGCCGCCGGGACCAGGCGTAATGTATGAGGCGCGTTTAGCCGCGTCTTCAAACACGACGTCGCCCACAACTTTGCCATTTTCCAGACGGTTGATGCCGACATCAATCACAATTGCGCCTTCTTTGATCCAGTCACCGGGAATAAAGCCTGGCTTGCCAACGGCAACGATCAATAGATCGGCATTTTCTAAGTGATGACGCAGAt                                                                                                                                                                                                                                    <  2:345602/251‑1 (MQ=255)
             aGCGTGTTTTCAATCAGCGTGGCAACCGTCATCGGGCCAACGCCGCCGGGAACAGGCGTAATGTATGAGGCGCGTTTAGCCGCGTCTTCAAACACGACGTCGCCCACAACTTTGCCATTTTCCAGACGGTTGATGCCGACATCAATCACAATTGCGCCTTCTTTGATCCAGTCACCGGGAATAAAGCCTGGCTTGCCAACGGCAACGCTCAATAGATCGGCATTTTCTAAGTGATGACGCAGATTTTTAGt                                                                                                                                                                                                                             >  2:162748/1‑251 (MQ=255)
                gtgtTTTCAATCAGCGTGGCAACCGTCATCGGGCCAACGCCGCCGGGAACAGGCGTAATGTATGAGGCGCGTTTAGCCGCGTCTTCAAACACGACGTCGCCCACAACTTTGCCATTTTCCAGACGGTTGATGCCGACATCAATCACAATTGCGCCTTCTTTGATCCAGTCACCGGGAATAAAGCCTGGCTTGCCAACGGCAACGATCAATAGATCGGCATTTTCTAAGTGATGACGCAGATTTTTAGTGaa                                                                                                                                                                                                                          <  1:127583/251‑1 (MQ=255)
                gtgtTTTCAATCAGCGTGGCAACCGTCATCGGGCCAACGCCGCCGGGAACAGGCGTAATGTATGAGGCGCGTTTAGCCGCGTCTTCAAACACGACGTCGCCCACAACTTTGCCATTTTCCAGACGGTTGATGCCGACATCAATCACAATTGCGCCTTCTTTGATCCAGTCACCGGGAATAAAGCCTGGCTTGCCAACGGCAACGATCAATAGATCGGCATTTTCTAAGTGATGACGCAGATTTTTAGTGaa                                                                                                                                                                                                                          <  1:15039/251‑1 (MQ=255)
                gtgtTTGCAATCAGCGTGGCAACCGTCATCGGGCCAACGCCGCCGGGAACAGGCGTAATGTATGAGGCGCGTTTAGCCGCGTCTTCAAACACGACGTCGCCCACAACTTTGCCATTTTCCAGACGGTTGATGCCGACATCAATCACAATTGCGCCTTCTTTGATCCAGTCACCGGGAATAAAGCCTGGCTTGCCAACGGCAACGATCAATAGATCGGCATTTTCTAAGTGATGACGCAGATTTTTAGTGaa                                                                                                                                                                                                                          <  1:59630/251‑1 (MQ=255)
                  gtTTTCAATCAGCGTGGCAACCGTCATCGGGCCAACGCCGCCGGGAACAGGCGTAATGTATGAGGCGCGTTTAGCCGCGTCTTCAAACACGACGTCGCCCACAACTTTGCCATTTTCCAGACGGTTGATGCCGACATCAATCACAATTGCGCCTTCTTTGATCCAGTCACCGGGAATAAAGCCTGGCTTGCCAACGGCAACGATCAATAGATCGGCATTTTCTAAGTGATGACGCAGATTTTTAGTGAAGc                                                                                                                                                                                                                        <  1:261004/251‑1 (MQ=255)
                           cAGCGTGGCAACCGTCATCGGGCCAACGCCGCCGGGAACAGGCGTAATGTATGAGGCGCGTTTAGCCGCGTCTTCAAACACGACGTCGCCCACAACTTTGCCATTTTCCAGACGGTTGATGCCGACATCAATCACAATTGCGCCTTCTTTGATCCAGTCACCGGGAATAAAGCCTGGCTTGCCAACGGCAACGATCAATAGATCGGCATTTTCTAAGTGATGACGCAGATTTTTAGTGAAGCGGTGAGTc                                                                                                                                                                                                                <  1:47235/250‑1 (MQ=255)
                                 ggCAACCGTCATCGGGCCAACGCCGCCGGGAACAGGCGTAATGTATGAGGCGCGTTTAGCCGCGTCTTCAAACACGACGTCGCCCACAACTTTGCCATTTTCCAGACGGTTGATGCCGACATCAATCACAATTGCGCCTTCTTTGATCCAGTCACCGGGAATAAAGCCTGGCTTGCCAACGGCAACGATCAATAGATCGGCATTTTCTAAGTGATGACGCAGATTTTTAGTGAAGCGGGGAGTCACTGtcg                                                                                                                                                                                                         >  2:66387/1‑249 (MQ=255)
                                        gTCATCGGGCCAACGCCGCCGGGAACAGGCGTAATGTATGAGGCGCGTTTAGCCGCGTCTTCAAACACGACGTCGCCCACAACTTTGCCATTTTCCAGACGGTTGATGCCGACATCAATCACAATTGCGCCTTCTTTGATCCAGTCACCGGGAATAAAGCCTGGCTTGCCAACGGCAACGATCAATAGATCGGCATTTTCTAAGTGATGACGCAGATTTTTAGTGAAGCGGTGAGTCACTGTAGTGGTGc                                                                                                                                                                                                   >  1:147680/1‑250 (MQ=255)
                                                             ggAACAGGCGTAATGTATGAGGCGCGTTTAGCCGCGTCTTCAAACACGAAGTCGCCCACAACTTTGCCATTTTCCAGACGGTTGATGACGAAATCAATCACAATTGCGCCTTCTTTGATCCAGTCACCGGGAATCAAGCCTGGCTTGCCAACGGCAACGATCAATAGATCGGCATTTTCTAAGTGATGACGCAGATTTTTAGTGAAGCGGTGAGTCACTGTAGTGGTGCAACCTGCCAGCAGCAGTTCCAt                                                                                                                                                                             >  1:199826/1‑251 (MQ=255)
                                                              gAACAGGCGTAATGTATGAGGCGCTTTTAGCCGCGTCTTCAAACACGACGTCGCCCACAACTTTGCCATTTTCCAGACGGTTGATGCCGACATCAATCACAATTGCGCCTTCTTTGATCCAGTCACCGGGAATAAAGCCTGGCTTGCCAACGGCAACGATCAATAGATCGGCATTTTCTAAGTGATGACGCAGATTTTTAGTGAAGCGGTGAGTCACTGGAGTGGTGCAACCTGCCAGCAGCAGTTCCATg                                                                                                                                                                            <  1:121880/251‑1 (MQ=255)
                                                                   ggCGTAATGTATGAGGCGCGTTTAGCCGCGTCTTCAAACACGACGTCGCCCACAACTTTGCCATTTTCCAGACGGTTGATGCCGACATCAATCACAATTGCGCCTTCTTTGATCCAGTCACCGGGAATAAAGCCTGGCTTGCCAACGGCAACGATCAATAGATCGGCATTTTCTAAGTGATGACGCAGATTTTTAGTGAAGCGGTGAGTCACTGTAGTGGTGCAACCTGCCAGCAGCAGTTCCATGCTCAt                                                                                                                                                                       >  2:247469/1‑251 (MQ=255)
                                                                   ggCGTAATGTATGAGGCGCGTTTAGCCGCGTCTTCAAACAAGACGTCGCCCCCAACTTTGCCATTTTCCCGACGGTTGATGCCGACATCAATCACAATTGCGCCTTCTTTGATCCAGTCACCGGGCATAAAGCCTGGCTTGCCAACGGCAACGATCAATAGATCGGCATTTTCTAAGTGATGACGCAGATTTTTAGTGAAGCGGTGAGTCACTGTAGTGGTGCAACCTGCCAGCAGCAGTTCCATGCTCAt                                                                                                                                                                       >  2:23982/1‑251 (MQ=255)
                                                                       tAATGTATAAGGAGCGTTTAGCCGCGTCTTCAAACACGACGTCGCCCACAACTTTGCCATTTTCCAGACGGTTGATGCCGACATCAATCACAATTGCGCCTTCTTTGATCCAGTCACCGGGAATAAAGCCTGGCTTGCCAACGGCAACGATCAATAGATCGGCATTTTCTAAGTGATGACGCAGATTTTTAGTGAAGCGGTGAGTCACTGTAGTGGTGCACCCTGCCAGCAGCAGTTCCATGCTCATCggg                                                                                                                                                                   >  2:235102/1‑251 (MQ=255)
                                                                             aTGAGGCGCGTTTAGCCGCGTCTTCAAACACGACGTCGCCCACAACTTTGCCATTTTCCAGACGGTTGATGCCGACATCAATCACAATTGCGCCTTCTTTGATCCAGTCACCGGGAATAAAGCCTGGCTTGCCAACGGCAACGATCAATAGATCGGCATTTTCTAAGTGATGACGCAGATTTTTAGTGAAGCGGTGAGTCACTGTAGTGGTGCAACCTGCCAGCAGCAGTTCCATGCTCATCGGGCGGCCa                                                                                                                                                             <  1:451505/251‑1 (MQ=255)
                                                                               gAGGCGCGTTTAGCCGCGTCTTCAAACACGACGTCGCCCACCACTTTGCCATTTTCCAGACGGTTGATGCCGACATCAATCACAATTGCGCCTTCTTTGATCCCGTCAACGGGCATAAAGCCTGGCTTGCCCACGGCAACGATCAATAGATCGGCATTTTCTAAGTGATGACGCAGCTTTTTAGTGAAGCGGTGCGTCACTGTAGTGGTGCAACCTGCCAGCAGCAGTTCCATGCTCCTCGGGCGGCCAAc                                                                                                                                                           >  1:172590/1‑251 (MQ=255)
                                                                               gAGGCGCGTTTAGCCGCGACTTCAAACACGACGTCGCCCACAACTTTGCCATTTTCCAGACGGTTGATGCCGACATCAATCACAATTGCGCCTTCTTTGATCCAGTCACCGGGAATAAAGCCTGGCTTGCCAACGGCAACGATCAATAGATCGGCATTTTCTAAGTGATGACGCAGATTTTTAGTGAAGCGGTGAGTCACTGTAGTGGTGCAACCTGCCAGCAGCAGTTCCATGCTCATCGGGCGGCCAAc                                                                                                                                                           >  1:93970/1‑251 (MQ=255)
                                                                                 ggCGCGTTTAGCCGCGTCTTCAAACACGACGTCGCCCACAACTTTGCCATTTTCCAGACGGTTGATGCCGACATCAATCACAATTGCGCCTTCTTTGATCCAGTCACCGGGAATAAAGCCTGGCTTGCCAACGGCAACGATCAATAGATCGGCATTTTCTAAGTGATGACGCAGATTTTTAGTGAAGCGGTGAGTCACTGTAGTGGTGCAACCTGCCAGCAGCAGTTCCATGCTCATCGGGCGGCCAACg                                                                                                                                                          >  1:335705/1‑250 (MQ=255)
                                                                                    gcgTTTAGCCGCGTCTTCAAACACGACGTCCCCCACAACTTTGCCATTTTCCAGACGGTTGATGCCGACATCAATCACAATTGCGCCTTCTTTGATCCAGTCACCGGGAATAAAGCCTGGCTTGCCAACGGAAACGATCAATAGATCGGAATTTTCTAAGTGATGACGCAGATTTTTAGTGAAGCGGTGAGTCACTGTAGTGGTGCAACCTGCCAGCAGCAGTTCCATGCTCATCGGGAGGCCAACGatat                                                                                                                                                      >  1:133213/1‑251 (MQ=255)
                                                                                          aGCCGCGTCTTCAAACACGACGTCGCCCACAACTTTGCCATTTTCCAGACGGTTGATGCCGACATCAATCACAATTGCGCCTTCTTTGATCCAGTCACCGGGAATAAAGCCTGGCTTGCCAACGGCAACGATCAATAGATCGGCATTTTCTAAGTGATGACGCAGATTTTTAGTGAAGCGGTGAGTCACTGTAGTGGTGCAACCTGCCAGCAGCAGTTCCATGCTCATCGGGCGGCCAACGATATTCGATg                                                                                                                                                <  2:147680/251‑1 (MQ=255)
                                                                                          aGCCGCGTCTTCAAACACGACGTCGCCCACAACTTTGCCATTTTCCAGACGGTTGATGCCGACATCAATCACAATTGCGCCTTCTTTGATCCAGTCACCGGGAATAAAGCCTGGCTTGCCAACGGCAACGATCAATAGATCGGCATTTTCTAAGTGATGACGCAGATTTTTAGTGAAGCGGTGAGTCACTGTAGTGGTGCAACCTGCCAGCAGCAGTTCCATGCTCATCGGGCGGCCAACGATATTCGATg                                                                                                                                                <  1:196404/251‑1 (MQ=255)
                                                                                                gTCTTCAAACACGACGTCGCCCACAACTTTGCCCTTTTCAAGACGGTTGATGCCGACATCAATCACAATTGCGCCTTCTTTGATCCAGTCACCGGGAATAAAGCCTGGCTTGCCAACGGCAACGATCAATAGATCGGCATTTTCTAAGTGATGACGCAGATTTTTAGTGAAGCGGTGAGTCACTGTAGTGGTGCAACCTGCCAGCAGCAGTTCCATGCTCATCGGGCGGCCAACGATATTCGCTGCGCCa                                                                                                                                           >  2:501686/1‑250 (MQ=255)
                                                                                                           cgacgTCGCCCACAACTTTGCCATTTTCCAGACGGTTGATGCCGACATCAATCACAATTGCGCCTTCTTTGATCCAGTCACCGGGAATAAAGCCTGGCTTGCCAACGGCAACGATCAATAGATCGGCATTTTCTAAGTGATGACGCAGATTTTTAGTGAAGCGGTGAGTCACTGTAGTGGTGCAACCTGCCAGCAGCAGTTCCATGCTCATCGGGCGGCCAACGATATTCGATGCGCCAATCACCACGGCg                                                                                                                               <  1:17968/251‑1 (MQ=255)
                                                                                                            gacgTCGCCAACAACTTTGCCATTTTCCAGACGGTTGATGCCGACATCAATCACAATTGCGCCTTCTTTGATCCAGTCACCGGGAATAAAGCCTGGCTTGCCAACGGCAACGATCAATAGATCGGCATTTTCTAAGTGATGACGCAGCTTTTTAGTGAAGCGGTGAGTCACTGTAGTGGTGCAACCTGCCAGCAGCAGTTCCATGCTCATCGGGCGGCCAACGATATTCGATGCGCCAATCACCACGGCGt                                                                                                                              >  2:173496/1‑251 (MQ=255)
                                                                                                               gTCGCCCCCAACTTTGCCAGTTTCCAGACGGTTGATGCCGACATCAATCACAATTTCGCCTTCTTTGATCCAGTCACCGGGAATAAAGCCTGGCTTGCCAACGGCAACGATCAATAGATCGGCATTTTCTAAGTGATGACGCAGATTTTTAGTGAAGCGGTGAGTCACTGTAGTGGTGCAACCGGCCAGCAGCAGTTCCATGCTCATCGGGCGGCCAACGATATTCGATGCGCCAATCACCACGGCGTTGa                                                                                                                           <  1:23982/251‑1 (MQ=255)
                                                                                                                     cccaACTCTGCCAGTTGCCAGAAGTTTGATGCCGGCATCAATCACAATTGCGCCTTCTTAGAGCCAGTCACCGGGAATAAAGACTGGCTTGCCAACAGCTACGATCAATAGATCGGCATTTTCTAAGTAATGACGCAGATTTTTAGTGAAGCGGTGAGTCACTGTAGTGGTGCAACCTGCCAGCAGCAGTTCCATGCTCATCGGGCGGCCAACGATATTCGATGCGCCAATCACCACGGCGTTGAGGCCGa                                                                                                                     <  1:469200/249‑1 (MQ=255)
                                                                                                                      ccaACGTTGCCATTTTCCAGACGGTTGATGCCGACATCAATCACAATTGCGCCTTCTTTGATCCAGTCACCGGGAATAAAGCCTGGCTTGCCAACGGCAACGATCAATAGATCGGCATTTTCTAAGTGATGACGCAGATTTTTAGTGAAGCGGTGAGTCACTGTAGTGGTGCAACCTGCCAGCAGCAGTTCCATGCTCATCGGGCGGCCAACGATATTCGATGCGCCAATCACCACGGCGTTGAGGCCGaa                                                                                                                    <  1:475244/250‑1 (MQ=255)
                                                                                                                        aaCTTTGCCATTTTCCAGACGGTTGATGCCGACATCAATCACAATTGCTCCTTCTTTGATCCAGTCACCGGGAATAAAGCCTGGCTTGCCAACGGCCACGATCACTAGATCGGCCTTTTCTAAGTGATGACGCAGATTTTTAGTGAAGCGGTGAGTCACTGTAGTGGTGCAACCTGCCAGCAGCAGTTCCCTGCTCATCGCGCGGCCAACGCTATTCGCTGCGCCAATCACCACGCCGTTGAGGCCGAAgg                                                                                                                  >  1:44887/1‑251 (MQ=255)
                                                                                                                           tttGCCATTTTCCAGACGGTTGATGCCGACATCAATCACAATTGCGCCTTCTTTGATCCAGTCACCGGGAATAAAGCCTGGCTTGCCAACGGCAACGATCAATAGATCGGCATTTTCTAAGTGATGACGCAGATTTTTAGTGAAGCGGTGAGTCACTGTAGTGGTGCAACCTGCCAGCAGCAGTTCCATGCTCATCGGGCGGCCAACGATATTCGATGCGCCAATCACCACGGCGTTGAGGCCGAAGGTAt                                                                                                               >  1:189695/1‑251 (MQ=255)
                                                                                                                              gCCATTTTCCAGACGGTTGATGCCGACATCAATCACAATTGCGCCTTCTTTGATCCAGTCACCGGGAATAAAGCCTGGCTTGCCAACGGCAACGATCAATAGATCGGCATTTTCTAAGTGATGACGCAGATTTTTAGTGAAGCGGTGAGTCACTGTAGTGGTGCAACCTGCCAGCAGCAGTTCCATGCTCATCGGGCGGCCAACGATATTCGATGCGCCACTCACCACGGCGTTGAGGCCGAAGGTATCa                                                                                                             >  1:162945/1‑250 (MQ=255)
                                                                                                                                cATTTTCCAGACGGTTGATGCCGACCTCAATCACAATTGCGCCTTCTTTGATCCAGTCACCGGGAATAAAGCCTGGCTTGCCAAAGGCAACGATCAATAGATCGGCATTTTCTAAGTGATGACGCAGATTTTTAGTGAAGCGGTGAGTCACTGTAGTGGTGCAACCTGCCAGCAGCAGTTCCATGCTCATCGGGCGGCCAACGATATTCGATGCGCCAATCACCACGGCGTTGAGGCCGAAGGTAGCAAtg                                                                                                          >  2:52843/1‑251 (MQ=255)
                                                                                                                                      ccAGACGGTGGATGCCGACATCAATCACAATTGCGCCTTCTTTGATCCAGTCACCGGGAATAAAGCCTGGCTTGCCAACGGCAGCGATCAATAGATCGGCATTTTCTAAGCGATGACGCAGAGTTTTAGTGAAGCGGTGAGTCACTGTAGTGGTGCAACCTGCAAGCAGCAGTTCCATGCTCATCGGGCGACCAACGATATTCGATGCGCCAATCAACACGGCGTTGCGGCCGAAGGTATCAATGTTGTac                                                                                                    >  2:427302/1‑250 (MQ=255)
                                                                                                                                        aGACGGTTGATGCCGACATCAATCACAATTGCGCCTTCTTTGATCCAGTCACCGGGAATAAAGCCTGGCTTGCCAACGGCAACGATCAATAGATCGGCATTTTCTAAGTGATGACGCAGATTTTTAGTGAAGCGGTGAGTCACTGTAGTGGTGCAACCTGCCAGCAGCAGTTCCATGCTCATCGTGCGGCCAACGATATTCGATGCGCCAATCACCACGGCGTTGAGGCCGAAGGTATCAATGTTGTAACg                                                                                                  <  1:470686/251‑1 (MQ=255)
                                                                                                                                            ggttgagccagACATCAATCAACATTGCGCATTCATTGAACCAGTGACCGGGAATCAAGCCTGACTTGCCCACGGAAACGATCCATAGAACGGCATTTTCTAAGTAATGACGCAGATTTTTAGTGAAGCGGAGCGTCACTGTAGTGGAGCAACCTGCAAGAAGCAGTTCCCTGCTCATCGGGCGGCCAACGATATTCGATGCGCGAATCACCAAGGCGTTGAGGCCGCCGGTATCAAGGTTGTAACGCTc                                                                                               >  2:207615/11‑250 (MQ=255)
                                                                                                                                             gTTGATGCCGACATCAATCACAATTGCGCCTTCGTTGATCCAGTCACCGGGAATAAAGCCTGGCTTGCCAACGGCAACGATCAATAGATCGGCATTTTCTAAGTGATGACGCAGATTTTTAGTGAAGCGGTGAGTCACTGTAGTGGTGCAACCTGCCAGCAGCAGTTCCATGCTCATCGGGCGGCCAACGATATTCGATGCGCCAATCACCACGGCGTTGAGGCCGAAGGTATCAATGTTGTAACGCTCaa                                                                                             <  1:448885/251‑1 (MQ=255)
                                                                                                                                                gattccGACATCAATCACAATTGCGCCTTCTTTGATCCAGTCACCGGGAATAAAGCCTGGCTTGCCAACGGCAACGATCAATAGATCGGCCTTTTCTAAGTGATGACGCAGATTTTTAGTGAAGCGGGGAGTAACTGTAGTGGTGCAACCTGCCAGCAGCAGTTCCATGCTCATCGGGCGGCCAACGATATTAGATGCGCAAATCACCACGGCGGTGAGGCCGCAGGGATCAATGTTGTCACGCACAagc                                                                                           >  2:44910/5‑250 (MQ=255)
                                                                                                                                                     cGACATCAATCACAATTGCGCCTTCTTTGATCCAGTCACCGGGAATAAAGCCTGGCTTGCCAACGGCAACGATCAATAGATCGGCATTTTCTAAGTGATGACGCAGATTTTTAGTGAAGCGGTGAGTCACTGTAGTGGTGCAACCTGCCAGCAGCAGTTCCATGCTCATCGGGCGGCCAACGATATTCGATGCGCCAATCACCACGGCGTTGAGGCCGAAGGTATCAATGTTGTAACGCTCAAGCAGCGTg                                                                                     <  1:381214/251‑1 (MQ=255)
                                                                                                                                                          tcaatcaCAATTGCGCCTTCTTTGATCCAGTCACCGGGAATAAAGCCTGGCTTGCCAACGGCAACGATCAATAGATCGGCATTTTCTAAGTGATGACGCAGATTTTTAGTGAAGCGGTGAGTCACTGTAGTGGTGCAACCTGCCAGCAGCAGTTCCATGCTCATCGGGCGGCCAACGATATTCGATGCGCCAATCACCACGGCGTTGAGGCCGAAGGTATCAATGTTGTAACGCTCAAGCAGCGTGACGAt                                                                                >  1:58202/1‑251 (MQ=255)
                                                                                                                                                           caatcaCAATTGCGCCTTCTTTGATCCAGTCACCGGGAATAAAGCCTGGCTTGCCAACGGCAACGATCAATAGATCGGCAGTGTCTAAGTGATGACGCAGATTTTTAGTGAAGCGGTGAGGCACTGTAGTGGTGCAACCTGCCAGCAGCAGTTCCATGCTCATCGGGCGGCCAACGATATTCGATGCGCCAATCACCACGGCGTTGAGGCCGAAGGTATCAATGTTGTAACGCTCAAGCAGCGTGACGAt                                                                                <  2:207233/250‑1 (MQ=255)
                                                                                                                                                                         ccTTCTTTGATCCAGTCACCGGGAATAAAGCCTGGCTTGCCAACGGCAACGATCAATAGATCGGCATTTTCTAAGTGATGACGCAGATTTTTAGTGAAGCGGTGAGTCACTGTAGTGGTGCAACCTGCCAGCAGCAGTTCCATGCTCATCGGGCGGCCAACGATATTCGATGCGCCAATCACCACGGCGTTGAGGCCGAAGGTATCAATGTTGTAACGCTCAAGCAGCGTGACGATACCGCGCGGGGTGCa                                                                 <  1:439548/251‑1 (MQ=255)
                                                                                                                                                                         ccTTCTTTGATCCAGTCACCGGGAATAAAGCCTGGCTTGCCAACGGCAACGATCAATAGATCGGCATTTTCTAAGGGCTGACGAAGATTTTTCGTGGAGCGGTGAGTCACTGTAGTGGTGCAACCTGCCAGCAGCAGTTCCATGCTCATCGGGCGGCCACCGATATTCGATGCGACAATCACCACGGCGTTGATGCCGGAGGCATCAATGTTGTAACGCTCAAGCAGCGAGACGATACCGCGCCGGGTGc                                                                  >  1:494990/1‑250 (MQ=255)
                                                                                                                                                                                tGATCCAGTCTACGGGAATAAAGCCTGGCTTGCCAACGGCAACGATCAATAGATCGGCATTTTCTCAGTGATGACGCAGATTTTTAGTGAAGCGGTGAGTCACTGTAGTGGTGCAACCTGCCAGCCGCAGTTCCATGCTCATCGGGAGGCCAACGATATTCGATGCGCCAATCACCACGGCGTTGAGGCCGAAGGTATCAATGTTGTAACGATAAAGCAGCGTGACGCTACCGCGCGGGGTGCAGGGACGc                                                          >  2:72140/1‑251 (MQ=255)
                                                                                                                                                                                  aTCCAGTCACCGGGAATAAAGCCTGGCTTGCCAACGGCAACGATCAATAGATCGGCATTTTCTAAGTGATGACGCAGATTTTTAGTGAAGCGGTGAGTCACTGTAGTGGTGCAACCTGCCAGCAGCAGTTCCATGCTCATCGGGCGGCCAACGATATTCGATGCGCCAATCACCACGGCGTTGAGGCCGAAGGTATCAATGTTGTAACGCTCAAGCAGCGTGACGATACCGCGCGGGGTGCAGGGACGc                                                          >  1:300628/1‑249 (MQ=255)
                                                                                                                                                                                    ccAGTCACCGGGAATAAAGCCTGGCTTGCCAACGGCAACGATCAATAGATCGGCATTTTCTAAGTGATGACGCAGATTTTTAGTGAAGCGGTGAGTCACTGTAGTGGTGCAACCTGCCAGCAGCAGTTCCATGCTCATCGGGCGGCCAACGATATTCGATGCGCCAATCACCACGGCGTTGAGGCCGAAGGTATCAATGTTGTAACGCTCAAGCAGCGTGACGATACCGCGCGGGGTGCAGGGACGCAGAc                                                      <  2:243089/251‑1 (MQ=255)
                                                                                                                                                                                      aGTCACCGGGACTAAAGCCTGGCTTGCCAACGGCAACGATCAATAGATCGGCATTTTCTAAGTGATGACGCAGATTTTTAGTGAAGCGGTGAGTCACTGTAGTGGTGCAACCTGCCAGCAGCAGTTCCATGCTCATCGGGCGGCCAACGATATTCGATGCGCCAATCACCACGGCGTTGAGGCCGAAGGTATCAATGTTGTAACGCTCAAGCAGCGTGACGATACCGCGCGGGGTGCAGGGACGCAGAcgc                                                    >  2:368454/1‑251 (MQ=255)
                                                                                                                                                                                       gtaaCCGGGAATAAAGCCTGGCTTGCCAACGGAAACGATCAATAGATCGGCATTTTCTAAGTGATGACGCAGATTTTTAGTGAAGCGGTGAGTCACTGTAGTGGTGCAACCTGCCAGCAGCAGTTCCATGCTCATCGGGCGGCCAACGATATTCGATGCGCCAATCACCACGGCGTTGAAGCCGAAGGCATCAATGTTGTAACGCTCAAGCAGCGTGCCGATACCGCGCGGGGTGCAGGGACGCAGAcgcg                                                   >  2:116239/4‑251 (MQ=255)
                                                                                                                                                                                       gTCACCGGGAATAAAGCCTGGCTTGCCAACGGCAACGATCAATAGATCGGCATTTTCTAAGTGATGACGCAGATTTTTAGTGAAGCGGTGAGTCACTGTAGTGGTGCAACCTGCCAGCAGCAGTTCCATGCTCATCGGGCGGCCAACGATATTCGATGCGCCAATCACCACGGCGTTGAGGCCGAAGGTATCAATGTTGTAACGCTCAAGCAGCGTGACGATACCGCGCGGGGTGCAGGGACGCAGAcgcg                                                   >  1:479460/1‑251 (MQ=255)
                                                                                                                                                                                         cACCGGGAAGAAAGCCTGGCTTGCCAACGGCAACGATCAATAGAGCGGCATTTTCTAAGTGATGACGCAGATTTTTAGTGAAGGGGTGAGTCACGGTAGTGGTGCAACCTGCCAGCAGCAGGTCCATGCTCATCGTGCGGCCAACGATATACGATGCGCCAATCACCACGGCGTTGAGGCCTAAGGTAGCAATGTTGGAACGCTCAAGCAGCGTGACGATATCGCGCGGGGTGCAGTGACGCAGAcgcg                                                   <  2:293319/249‑1 (MQ=255)
                                                                                                                                                                                          aCCGGGAATAAAGCCTGGCTTGCCAACGGCAACGATCAATAGATCGGCATTTTCTAAGTGATGACGCAGATTTTTAGTGAAGCGGTGAGTCACTGTAGTGGTGCAACCTGCCAGCAGCAGTTCCATGCTCATCGGGCGGCCAACGATATTCGATGCGCCAATCACCACGGCGTTGAGGCCGAAGGTATCAATGTTGTAACGCTCAAGCAGCGTGACGATACCGCGCGGGGTGCAGGGACGCAGACgcggcg                                                >  2:137357/1‑251 (MQ=255)
                                                                                                                                                                                                aaTAAAGCCTGGCTTGCCAACGGCAACTATCAATAGATCGGGATTTTCGAAGTGATGACGCAGATTTTTAGTGAAGCGGTGAGTCACTGTAGGTGTGCAACCTGCCAGCAGCAGTTCCATGCTCATCTGGCGGCCAACGATATTCGATGCGCCAAGCACCACGGCGTTGAGGCGGAAGGTATCAATGTTGTAACGCTCAAGCAGCGTGACGATACCGCGCGGGGTGCAGGGAGGCTGACGCTGCGTGCGCt                                          <  2:199826/251‑1 (MQ=255)
                                                                                                                                                                                                 aTAAAGCCTGGCTTGCCAACGGCAACGATCAATAGATCGGCATTTTCTAAGTGATGACGCAGATTTTTAGTGAAGCGGTGAGTCACTGTAGTGGTGCAACCTGCCAGCAGCAGTTCCATGCTCATCGGGCGGCCAACGATATTCGATGCGCCAATCACCACGGCGTTGAGGCCGAAGGTATCAATGTTGTAACGCTCAAGCAGCGTGACGATACCGCGCGGGGTGCAGGGACGCAGACGCGGCGCGCGCTg                                         >  1:501319/1‑251 (MQ=255)
                                                                                                                                                                                                  tAAAGCCTGGCTTGCCAACGGCAACGATCAATAGATCGGCATTTTCTAAGTGATGACGCAGATTTTTAGTGAAGCGGTGAGTCACTGTAGTGGTGCAACCTGCCAGCAGCAGTTCCATGCTCATCGGGCGGCCAACGATATTCGATGCGCCAATCACCACGGCGTTGAGGCCGAAGGTATCAATGTTGTAACGCTCAAGCAGCGTGACGATACCGCGCGGGGTGCAGGGACGCAGACGCGGCGCGCGCTgg                                        >  1:134178/1‑251 (MQ=255)
                                                                                                                                                                                                   aaaGCCTGGCTTGCCAACGGCAACGATCAATAGATCGGCATTTTCTAAGTGATGACGCAGATTTTTAGTGAAGCGGTGAGTCACTGTAGTGGTGCAACCTGCCAGCAGCAGTTCCATGCTCATCGGGCGGCCAACGATATTCGATGCGCCAATCACCACGGCGTTGAGGCCGAAGGTATCAATGTTGTAACGCTCAAGCAGCGTGACGATACCGCGCGGGGTGCAGGGACGCAGACGCGGCGCGCGCTgg                                        <  2:138914/250‑1 (MQ=255)
                                                                                                                                                                                                       ccTGGCTTGCCAACGGCAACGATCAATAGATCGGCATTTTCTAAGTGATGACGCAGATTTTTAGTGAAGCGGTGAGTCACTGTAGTGGTGCAACCTGCCAGCAGCAGTTCCATGCTCATCGGGCGGCCAACGATATTCGATGCGCCAATCACCACGGCGTTGAGGCCGAAGGTATCAATGTTGTAACGCTCAAGCAGCGTGACGATACCGCGCGGGGTGCAGGGCCGCAGCCGCGGCGCGCGCTGGcac                                     >  2:370055/1‑249 (MQ=255)
                                                                                                                                                                                                          ggCTTGCCAACGGCAACGATCAATAGATCGGCATTTTCTAAGGGATGACGCAGATTTTTAGTGAAGCGGTGAGTCACTGTAGTGGTGCAACCTGCCAGCAGCAGTTCCATGCTCATCGGGCGGCCAACGATATTCGATGCGCCAATCACCACGGCGTTGAGGCCGAAGGTATCAATGTTGTAACGCTCAAGCAGCGTGACGATACCGCGCGGGGTGCAGGGACGCAGACGCGGCGCGCGCTGGCACAgacg                                <  2:39381/251‑1 (MQ=255)
                                                                                                                                                                                                            cTTGCCAACGGCAACGATCAATAGATCGGCATTTTCTAAGTGATGACGCAGATTTTTAGTGAAGCGGTGAGTCACTGTAGTGGTGCAACCTGCCAGCAGCAGTTCCATGCTCATCGGGCGGCCAACGATATTCGATGCGCCAATCACCACGGCGTTGAGGCCGAAGGTATCAATGTTGTAACGCTCAAGCAGCGTGACGATACCGCGCGGGGTGCAGGGACGCAGACGCGGCGCGCGCTGGCACAgacgac                              >  1:146811/1‑251 (MQ=255)
                                                                                                                                                                                                               gCCAACGGCAACGATCAATAGATCGGCATTTTCTAAGTGATGACGCAGATTTTTAGTGAAGCGGTGAGTCACTGTAGTGGTGCAACCTGCCAGCAGCAGTTCCATGCTCATCGGGCGGCCAACGATATTCGATGCGCCAATCACCACGGCGTTGAGGCCGAAGGTATCAATGTTGTAACGCTCAAGCAGCGTGACGATACCGCGCGGGGTGCAGGGACGCAGACGCGGCGCGCGCTGGCACAGAcgaccg                            <  1:137357/250‑1 (MQ=255)
                                                                                                                                                                                                                 cAACGGCAACGATCAAGAGATCGGCATTTTCTAAGTGATGACGCAGATTTTTAGTGAAGCGGTGAGTCACTGTAGTGGTGCAACCTGCCAGCAGCAGTTCCATGCTCATCGGGCGGCCAACGATATTCGATGCGCCAATCACCACGGCGTTGAGGCCGAAGGTATCAATGTTGTAACGCTCAAGCAGCGTGACGATACCGCGCGGGGTGCAGGGACGCAGACGCGGCGCGCGCTGGCACAGACGACCGACg                         <  1:5531/251‑1 (MQ=255)
                                                                                                                                                                                                                     ggCAACGATCAATAGATCGGCATTTTCTAAGTGATGACGCAGATTTTTAGTGAAGCGGTGAGTCACTGTAGTGGTGCAACCTGCCAGCAGCAGTTCCATGCTCATCGGGCGGCCAACGATATTCGATGCGCCAATCACCACGGCGTTGAGGCCGAAGGTATCAATGTTGTAACGCTCAAGCAGCGTGACGATACCGCGCGGGGTGCAGGGACGCAGACGCGGCGCGCGCTGGCACAGACGACCGACGTTGt                     >  1:489607/1‑251 (MQ=255)
                                                                                                                                                                                                                        aaCGATCAATAGATCGGCATTTTCTAAGTGATGACGCAGATTTTTAGTGAAGCGGTGAGGCACTGTAGTGGTGCAACCTGCCAGCAGCAGTTCCATGCTCATCGGGCGGCCAACGATATTCGATGCGCCAATCACCACGGCGTTGAGGCCGAAGGTATCAATGTTGTAACGCTCAAGCAGCGTGACGATACCGCGCGGGGTGCAGGGACGCAGACGCGGCGCGCGCTGGCACAGACGACCGACGTTGTAAg                  <  2:162285/251‑1 (MQ=255)
                                                                                                                                                                                                                                aTAGAGCGGCATTTTCTAAGTGATGACGCATATTTTTAGGGAAGCGGTGGGTCACTGTAGGTGTGCAACTTGCCAGCAGCAGGTCCATGCTCAACGGGCGGCCAACGATATTCGATGCGCCAATCTCCACGGCGTTGAGTCCGAAGGTATCAATGTGGTAATGCGCAAGCAGCGTGACGATCCCGCGCGGGGTACAGGGACGCAGACGCTGCGCGCGCTGGCACAGACTACTGACGTTGTAAGGATGGaaa          <  2:502221/251‑1 (MQ=255)
                                                                                                                                                                                                                                   gATCTGCATTTTCTACGTGATGACGCAGATTTTTAGTGAAGCGGTGAGTCACTGTAGTGGTGACACCTGCCAGCAGCAGTTCCATGCTCATCGGGCGGCCAACGATATTCGATGCGCCAATCACCACGGCGTTGGGGCCGAAGGTATCAATGTTGTAACGCTCAAGCAGCGTGACGATACCGCGCGGGGTGCAGGGACGCAGCCGCGGCGAGCGCTGGCACAGACGACCGCCGTTGTACGGATGGAAAcgg       >  2:202445/1‑249 (MQ=255)
                                                                                                                                                                                                                                      cGGCATTTTCTAAGTGATGACGCAGATTTTTAGTGAAGCGGTGAGTCACTGTAGTGGTGCAACCTGCCAGCAGCAGTTCCATGCTCATCGGGCGGCCAACGATATTCGATGCGCCAATCACCACGGCGTTGAGGCCGAAGGTATCAATGTTGTAACGCTCAAGCAGCGTGACGATACCGCGCGGGGTGCAGGGACGCAGACGCGGCGCGCGCTGGCACAGACGACCGACGTTGTAAGGATGGAAACCGTcc    >  2:135072/1‑251 (MQ=255)
                                                                                                                                                                                                                                        gCATTTTCTAAGTGATGACGTAGATTTTTAATGAAGTGTTGATTCACTGTAGTGGTGCAACCTGCCAGCAGCAGTTTCAGGCTCATCGGGCGGCCAACGGTATTCGATGCGCCAATCACCACGGCGTTGAGGCCGTAGGTATCAATGTTGTAACGCGCAAGCAGCGTTACGATACCGCGCGGTGTGCAGGGACGCAGACTCGGCGCGCGCTGGCACAGACGACCGGCTTTGTTATGATGGAAACCGTc     <  2:251706/248‑1 (MQ=255)
                                                                                                                                                                                                                                        gCATTTTCTAAGTGATGACGCAGATTTTTAGTGAAGCGGTGAGTCACTGTAGTGGTGCAACCTGCCAGCAGCAGTTCCATGCTCATCGGGCGGCCAACGATATTCGATGCGCCAATCACCACGGCGTTGAGGCCGAAGGTATCAATGTTGTAACGCTCAAGCAGCGTGACGATACCGCGCGGGGTGCAGGGACGCAGACGCGGCGCGCGCTGGCACAGACGACCGACGTTGTAAGGATGGAAACCGTCCAc  <  2:103773/251‑1 (MQ=255)
                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                
AACGCACGCCTGTAGCGTGTTTTCAATCAGCGTGGCAACCGTCATCGGGCCAACGCCGCCGGGAACAGGCGTAATGTATGAGGCGCGTTTAGCCGCGTCTTCAAACACGACGTCGCCCACAACTTTGCCATTTTCCAGACGGTTGATGCCGACATCAATCACAATTGCGCCTTCTTTGATCCAGTCACCGGGAATAAAGCCTGGCTTGCCAACGGCAACGATCAATAGATCGGCATTTTCTACGTGATGACGCAGATTTTTAGTGAAGCGGTGAGTCACTGTAGTGGTGCAACCTGCCAGCAGCAGTTCCATGCTCATCGGGCGGCCAACGATATTCGATGCGCCAATCACCACGGCGTTGAGGCCGAAGGTATCAATGTTGTAACGCTCAAGCAGCGTGACGATACCGCGCGGGGTGCAGGGACGCAGACGCGGCGCGCGCTGGCACAGACGACCGACGTTGTAAGGATGGAAACCGTCCAC  >  NC_000913/556902‑557384

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑