Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913245,6620GA45.3% 12.4 / 71.5 53I48I (ATC→ATT) dpaApeptidoglycan meso‑diaminopimelic acid protein amidase A
Reads supporting (aligned to +/- strand):  ref base G (16/13);  new base A (15/9);  total (31/22)
Fisher's exact test for biased strand distribution p-value = 7.80e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.12e-01
Rejected as consensus: Frequency below/above cutoff threshold.

TTCCCTTCATAACCATGCGCACGGTCATAGGCATTGGGGAAACCAATATTAATCGCTTTGTAGTAACGGCTGTCTGGTTTTAACTGATTACGCTGGACGCTATAAAACCCTTCCGGGCTTTTGAAATCGCCCTGACGCTGTTTTGGCCCTAAGCCGCCGGAATATTTACAGATTTTATAGCTGTCGAGCAGTTGATATTGCTCGCCCATTTTGACGTAGAGATCGAGCGTACGTTCTTCCTTGAAGATCTGGATGTAGACAGGGGATCCCATCAACTGCTGCTTATACTCTTTGCTCACAGGCGTGGTAGAGCTACTGCTGCCCAGCAAACCGGCAAATGAAACGCACGGGATCAACAACATCGCAAGAATTAATGCGATTTTACGCATACTGCTTATTCCTTGATAAAACGGTTACACACGCCAGGACGGCAAAATGGATCCCAAATCGGAATAGTCTGGATTTGGAAGGCTCACATTATCACCAAA  >  NC_000913/245417‑245904
                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                  
ttCCCTTCATAACCATGCGCACGGTCATAGGCATTGGGGAAACCAATATTAATCGCTTTGTAGTAACGGCTGTCTGGTTTTAACTGATTACGCTGGACGCTATAAAACCCTTCCGGGCTTTTGAAATCGCCCTGACGCTGTTTTGGCCCTAAGCCGCCGGAATATTTACAGATTTTATAGCTGTCGAGCAGTTGATATTGCTCGCCCATTTTGACGTAGAGATCGAGCGTACGTTCTTCCTTGAAGATCTg                                                                                                                                                                                                                                               >  2:12666/1‑251 (MQ=255)
   ccTTCATAACCATGCGCACGGTCATAGGCATTGGGGAAACCAATATTAATCGCTTTGTAGTAACGGCTGTCTGGTTTTAACTGATTACGCTGGACGCTATAAAACCCTTCCGGGCTTTTGAAATCGCCCTGACGCTGTTTTGGCCCTAAGCCGCCGGAATATTTACAGATTTTATAGCTGTCGAGCAGTTGATATTGCTAGCCCATTTTGACGTAGAGATCGAGCGTACGTTCTTCCTTGAAAATCTGGAt                                                                                                                                                                                                                                            <  1:156583/251‑1 (MQ=255)
    cTTCATAACCATGCGCACGGTCATAGGCATTGGGGAAACCAATATTAATCGCTTTGTAGTAACGGCTGTCTGGTTTTAACTGATTACGCTGGACGCTATAAAACCCTTCCGGGCTTTTGAAATCGCCCTGACGCTGTTTTGGCCCTAAGCCGCCGGAATATTTACAGATTTTATAGCTGTCGAGCAGTTGATATTGCTCGCCCATTTTGACGTAGAGATCGAGCGTACGTTCTTCCTTGAAAATCTGGATg                                                                                                                                                                                                                                           >  1:106633/1‑251 (MQ=255)
            ccATGCGCACGGTCATAGGCATTGGGGAAACCAATATTAATCGCTTTGTAGTAACGGCTGTCTGGTTTTAACTGATTACGCTGGACGCTATAAAACCCTTCCGGGCTTTTGAAATCGCCCTGACGCTGTTTTGGCCCTAAGCCGCCGGAATATTTACAGATTTTATAGCTGTCGAGCAGTTGATATTGCTCGCCCATTTTGACGTAGAGATCGAGCGTACGTTCTTCCTTGAAGATCTGGATGTAGACAgg                                                                                                                                                                                                                                   <  1:363713/251‑1 (MQ=255)
              aTGCGCACGGTCATAGGCATTGGGGAAACCAATATTAATCGCTTTGTAGTAACGGCTGTCTGGTTTTAACTGATTACGCTGGACGCTATAAAACCCTTCCGGGCTTTTGAAATCGCCCTGACGCTGTTTTGGCCCTAAGCCGCCGGAATATTTACAGATTTTATAGCTGTCGAGCAGTTGATATTGCTCGCCCATTTTGACGTAGAGATCGAGCGTACGTTCTTCCTTGAAGATCTGGATGTAGACAgggg                                                                                                                                                                                                                                 >  2:84229/1‑251 (MQ=255)
                 cgcACGGTCATAGGCATTGGGGAAACCAATATTAATCGCTTTGTAGTAACGGCTGTCTGGTTTTAACTGATTACGCTGGACGCTATAAAACCCTTCCGGGCTTTTGAAATCGCCCTGACGCTGTTTTGGCCCTAAGCCGCCGGAATATTTACAGATTTTATAGCTGTCGAGCAGTTGATATTGCTCGCCCATTTTGACGTAGAGATCGAGCGTACGTTCTTCCTTGAAGATCTGGATGTAGACAGGGGATc                                                                                                                                                                                                                              >  2:363996/1‑251 (MQ=255)
                   cACGGTCATAGGCATTGGGGAAACCAATATTAATCGCTTTGTAGTAACGGCTGTCTGGTTTTAACTGATTACGCTGGACGCTATAAAACCCTTCCGGGCTTTTGAAATCGCCCTGACGCTGTTTTGGCCCTAAGCCGCCGGAATATTTACAGATTTTATAGCTGTCGAGCAGTTGATATTGCTCGCCCATTTTGACGTAGAGATCGAGCGTACGTTCTTCCTTGAAAATCTGGATGTAGACAGGGGATccc                                                                                                                                                                                                                            >  1:246398/1‑251 (MQ=255)
                          aTAGGCATTGGGGAAACCAATATTAATCGCTTTGTAGTAACGGCTGTCTGGTTTTAACTGATTACGCTGGACGCTATAAAACCCTTCCGGGCTTTTGAAATCGCCCTGACGCTGTTTTGGCCCTAAGCCGCCGGAATATTTACAGATTTTATAGCTGTCGAGCAGTTGATATTGCTCGCCCATTTTGACGTAGAGATCGAGCGTACGTTCTTCCTTGAAGATCTGGATGTAGACAGGGGATCCCATCAAct                                                                                                                                                                                                                     >  2:72487/1‑251 (MQ=255)
                                  tGGGGAAACCAATATTAATCGCTTTGTAGTAACGGCTGTCTGGTTTTAACTGATTACGCTGGACGCTATAAAACCCTTCCGGGCTTTTGAAATCGCCCTGACGCTGTTTTGGCCCTAAGCCGCCGGAATATTTACAGATTTTATAGCTGTCGAGCAGTTGATATTGCTCGCCCATTTTGACGTAGAGATCGAGCGTACGTTCTTCCTTGAAGATCTGGATGTAGACAGGGGATCCCATCAACTGCTGCtt                                                                                                                                                                                                              <  2:200353/250‑1 (MQ=255)
                                    gggAAACCAATATTAATCGCTTTGTAGTAACGGCTGGCTGGTTTTAACTGATTACGCTGGACGCTATAAAACCCTTCCGGGCTTTTGAAATCGCCCTGACGCTGTTTTGGCCCTAAGCCGCCGGAATATTTACAGATTTTATAGCTGTCGAGCAGTTGATATTTCTCGCCCATTTGGACGTAGAGATCGAGCGTACTTTCTTCCTTGAAGAGCTGGAGGTAGACAGGGGATCCCATCAACTTCTGCTtatc                                                                                                                                                                                                           <  2:66520/251‑2 (MQ=255)
                                     ggAAACCAATATTAATCGCTTTGTAGTAACGGCTGTCTGGTTTTAACTGATTACGCTGGACGCTATAAAACCCTTCCGGGCTTTTGAAATCGCCCTGACGCTGTTTTGGCCCTAAGCCGCCGGAATATTTACAGATTTTATAGCTGTCGAGCAGTTGATATTGCTCGCCCATTTTGACGTAGAGATCGAGCGTACGTTCTTCCTTGAAGATCTGGATGTAGACAGGGGATCCCATCAACTGCTGCTTATAc                                                                                                                                                                                                          >  1:368289/1‑251 (MQ=255)
                                      gAAACCAATATTAATCGCTTTGTAGTAACGGATGTCTGGTTTTAACTGATTACGCTGGACGCTATAAAACCCTTCCGGGCTTTTGAAATCGCCCTGACGCTGTTTTGGCCCTAAGCCGCCGGAATATTTACAGATTTTATAGCTGTCGAGCAGTTGATATTGCTCGCCCATTTTGACGTAGAGATCGAGCGTACGTTCTTCCTTGAAGATCTGGATGTAGACAGGGGATCACATCAACTGCTGCTTATAct                                                                                                                                                                                                         >  1:437761/1‑251 (MQ=255)
                                           cAATATTAATCGCTTTGTAGTAACGTCTGTCTGGTTTTAACTGATTACGCTGGACGCTATAAAACCCTTCCGGGCTTTTGAAATCGCCCTGACGCTGTTTTGGCCCTCAGCCGCCGGAATATTTACAGATTTTATAGCTGTCGAGCAGTTGATATTACTCGCCCATTTTGACGTAGAGATCGAGCGTACGTTCTTCCTTGAAAATCTGGATGTAGACAGGGGATCCCATCAACTGCTGCTTATACTCTTTg                                                                                                                                                                                                    >  1:254143/1‑251 (MQ=255)
                                              tatTAATCGCTTTGTAGTAACGGCTGTCTGGTTTTAACTGATTACGCCGGACGCTATAAAACCCTTCCGGGCTTTTGAAATCGCCCTGACGCTGTTTTGGCCCTAAGCCGCCGGAATATTTACAGATTTTATAGCTGTCGAGAAGTTGATATTGCTCGCCCATTTTGACGTAGAGATCGAGCGTACGTTCTTCCTTGAAGATCTGGATGTAGACAGGGGATCCCATCAACTGCTGCTTATACTCTTTGCTc                                                                                                                                                                                                 >  1:308479/1‑251 (MQ=255)
                                                 tAATCGCTTTGTAGTAACGGCTGTCTGGTTTTAACTGATTACGCTGGACGCTATAAAACCCTTCCGGGCTTTTGAAATCGCCCTGACGCTGTTTTGGCCCTAAGCCGCCGGAATATTTACAGATTTTATAGCTGTCGAGCAGTTGATATTGCTCGCCCATTTTGACGTAGAGATCGAGCGTACGTTCTTCCTTGAAAATCTGGATGTAGACAGGGGATCCCATCAACTGCTGCTTATACTCTTAGCTcac                                                                                                                                                                                               >  2:111774/1‑250 (MQ=255)
                                                    tCGCTTTGTAGTAACGGCTGTCTGGTTTTAACTGATTACGCTGGACGCTATAAAACCCTTCCGGGCTTTTGAAATCGCCCTGACGCTGTTTTGGCCCTAAGCCGCCGGAATATTTACAGATTTTATAGCTGTCGAGCAGTTGATATTGCTCGCCCATTTTGACGTAGAGATCGAGCGTACGTTCTTCCTTGAAAATCTGGATGTAGACAGGGGATCCCATCAACTGCTGCTTATACTCTTTGCTCACAGGc                                                                                                                                                                                           >  1:401675/1‑251 (MQ=255)
                                                      gCTTTGTAGTAACGGCTGTCTGGTTTTAACTGATTACGCTGGACGCTATAAAACCCTTCCGGGCTTTTGAAATCGCCCTGACGCTGTTTTGGCCCTAAGCCGCCGGAATATTTACAGATTTTATAGCTGTCGAGCAGTTGATATTGCTCGCCCATTTTGACGTAGAGATCGAGCGTACGTTCTTCCTTGAAGATCTGGATGTAGACAGGGGATCCCATCAACTGCTGCTTATACTCTTTGCTCACAGGCGt                                                                                                                                                                                         <  1:84229/251‑1 (MQ=255)
                                                        tttGTAGTAACGGCTGTGTGGTTTTAAATGATTACGCTGGTAGCTATAAAACGCTTCCGGGCTTTTGAAATCGCCCTGAAGATGTATTGGCACTAAGCCGAAGGATTATTTACAGCTTTTATAGCTGTCGAGCAGTTGAAATTGCTCGCCCATTTTGACGTAGAGATCGAGCGTACGTTCTTCCTTGACATTCTGGATGTAGTAAGGGGATACCATCACCTGCTGCTTATACTCTTTGCTCACAGGCGTgg                                                                                                                                                                                       >  2:259228/1‑251 (MQ=255)
                                                        tttGTAGTAACGGCTGTCTGGTTTTAACTGATTACGCTGGACGCTATAAAACCCTTCCGGGCTTTTGAAATCGCCCTGACGCTGTTTTGGCCCTAAGCCGCCGGAATATTTACAGATTTTATAGCTGTCGAGCAGTTGATATTGCTCGCCCATTTTGACGTAGAGATCGAGCGTACGTTCTTCCTTGAAAATCTGGATGTAGACAGGGGATCCCATCAACTGCTGCTTATACTCTTTGCTCACAGGCGTgg                                                                                                                                                                                       >  2:394522/1‑251 (MQ=255)
                                                          tGTAGTAACGGCTGTCTGGTTTTACCTGATTACGCTGGACGCTATAAAAACCTTCCGGGCTTTTGAAATCGCCCTGACGCTGTTTTGGCCCTAAGCCGCCGGAATATTTACAGATTTTATAGCTGTCGAGCAGTTGATATTGCTCGCACATTTTGACGTAGAGATCGAGCGTACGTTCTTCATTGAAGATCTGGATGTAAACAGGGGATCCCATCAACTGCTGCTTATACTCTTTGCTCACAGGCGTGGt                                                                                                                                                                                      >  2:408084/1‑250 (MQ=255)
                                                                 aCGGCTGTCTGGTTTTAACTGATTACGCTGGACGCTATAAAACCCTTCCGGGCTTTTGAAATCGCCCTGACGCTGTTTTGGCCCTAAGCCGCCGGAATATTTACAGATTTTATAGCTGTCGAGCAGTTGATATTGCTCGCCCATTTTGACGTAGAGATCGAGCGTACGTTCTTCCTTGAAGATCTGGATGTAGACAGGGGATCCCATCAACTGCTGCTTATACTCTTTGCTCACAGGCGTGGTAGAGCTAc                                                                                                                                                                              >  1:245211/1‑251 (MQ=255)
                                                                           ggTTTTAACTGATTACGCTGGACGCTATAAAACCCTTCCGGGCTTTTGAAATCGCCCTGACGCTGTTTTGGCCCTAAGCCGCCGGAATATTTACAGATTTTATAGCTGTCGAGCAGTTGATATTGCTCGCCCATTTTGACGTAGAGATCGAGCGTACGTTCTTCCTTGAAAATCTGGATGTAGACAGGGGATCCCATCAACTGCTGCTTATACTCTTTGCTCACAGGCGTGGTAGAGCTACTGCTGccc                                                                                                                                                                      >  2:382127/1‑249 (MQ=255)
                                                                           ggTTTTAACTGATTACGCTGGACGCTATAAAACCCTTCCGGGCTTTTGAAATCGCCCTGACGCTGTTTTGGCCCTAAGCCGCCGGAATATTTACAGATTTTATAGCTGTCGAGCAGTTGATATTGCTCGCCCATTTTGACGTAGAGAGCGAGCGTACGTTCTTCCTTGAAAATCTGGATGTAGACAGGGGATCCCATCAACTGCGGCTTATACTCTTTGCTCACAGGCGTGGTAGAGCTACTGCTGccc                                                                                                                                                                      <  1:467608/249‑1 (MQ=255)
                                                                                   ccgattactcttggcGCTATAAAACCCTTCCGGTCTGTTTAAATCGCCCTGACTCTGTTTTGGCCCTAAGCCGCCGGAATATTTACAGATTTTATAGCTGTCGAGCAGTTGATATTGCTCGCCCATTTTGACGTAGAGATCGAGCGTACGTTCTTCCTTGAAAATCTGGATGTAGACAGGGGATCCCATCAACTGCTGCTTATACTCTTGGCGCACAGGCGTTGTAGAGCTACTGCTGCCCAGCAAACCgc                                                                                                                                                            <  2:20698/237‑2 (MQ=255)
                                                                                          cGCTGGACGCTATAAAACCCTTCCGGGCTTTTGAAATCGCCCTGACGCTGTTTTGGCCCTAAGCCGCCGGAATATTTACAGATTTTATAGCTGTCGCGCAGTTGATATTGCTCGCCCATTTTGACGTAGAGATCGAGCGTACGTTCTTCCTTGAAAATCTGGATGTAGACAGGGGATCCCATCAACTGCTGCTTATACTCTTTGCTCACAGGCGTGGTAGAGCTACTGCTGCCCAGCAAACCGGCAAATg                                                                                                                                                      >  2:483112/1‑250 (MQ=255)
                                                                                                          ccccTTCCGGGCTTTTGAAATCTCCCTGACGCTGTTTTGGCCATAAGCCGCCGGAAGATTTACAGATTTTATAGCTGTCGAGCAGATGATATTGCTCGCCCATTTTGACGTAGAGATCGAGCGTACGTTCTCCCTTGAAAATCTGGATGTAGACAGGGGATCCCATCAACTGCTGCTTATACTCTTTGCTCACAGGCGTGGTAGAGCTACCGCTGCCCAGCAAACCGGCAAATGAACCGCCCGGGATcaac                                                                                                                                     <  2:254143/250‑1 (MQ=255)
                                                                                                            ccTTCCGGGCTTTTGAAATCGCCCTGACGCTGTTTTGGCCCTAAGCCGCCGGAATATTTACAGATTTTATAGCTGTCGAGCAGTTGATATTGCTCGCCCATTTTGACGTAGAGATCGAGCGTACGTTCTTCCTTGAAGATCTGGATGTAGACAGGGGATCCCATCAACTGCTGCTTATACTCTTTGCTCACAGGCGTGGTAGAGCTACTGCTGCCCAGCAAACCGGCAAATGAAACGCACGGGATcaacaa                                                                                                                                   <  2:435589/251‑1 (MQ=255)
                                                                                                                  gggCTTTTGAAATCGCCCTGACGCTGTTTTGGCCCTAAGCCGCCGGAATATTTACAGATTTTATAGCTGTCGAGCAGTTGATATTGCTCGCCCATTTTGACGTAGAGATCGAGCGTACGTTCTTCCTTGAAAATCTGGATGTAGACAGGGGATCCCATCAACTGCTGCTTATACTCTTTGCTCACAGGCGTGGTAGAGCTACTGCTGCCCAGCAAACCGGCAAATGAAACGCACGGGATCAACAACATCGc                                                                                                                             >  2:324657/1‑251 (MQ=255)
                                                                                                                          gAAATCGCCCTGACGCTGTTTTGGCCCTAAGCCGCCGGAATATTTACAGATTTTATAGCTGTCGAGCAGTTGATATTGCTCGCCCATTTTGACGTAGAGATCGAGCGTACGTTCTTCCTTGAAGATCTGGATGTAGACAGGGGATCCCATCAACTGCTGCTTATACTCTTTGCTCACAGGCGTGGTAGAGCTACTGCTGCCCAGCAAACCGGCAAATGAAACGCACGGGATCAACAACATCGCAAGAATTa                                                                                                                     <  1:14602/251‑1 (MQ=255)
                                                                                                                              tCGCCCTGACGCTGTTTTGGCCCTAAGCCGCCGGAATATTTTCAGCTTTTATAGCTGTCGAGCAGTTGATATTGCTCGCCCATTTTGACGTAGAGATCGAGCGTACGTTCTTCCTTGAAGATCTGGATGTAGACAGGGGATCCCATCAACTGCTGCTTATACTCTTTGCTCACAGGCGTGGTAGAGCTACTGCTGCCCAGCAAACCGGCAAATGAAACGCACGGGATCAACAACATCGCAAGAATTAATGc                                                                                                                 >  2:90113/1‑251 (MQ=255)
                                                                                                                                               tGGCCCTAAGCCGCCGGAATATTTACAGATTTTATAGCTGTCGAGCAGTTGATATTGCTCGCCCATTTTGACGTAGAGATCGAGCGTACGTTCTTCCTTGAAGATCTGGATGTAGACAGGGGATCCCATCAACTGCTGCTTATACTCTTTGCTCACAGGCGTGGTAGAGCTACTGCTGCCCAGCAAACCGGCAAATGAAACGCACGGGATCAACAACATCGCAAGAATTAATGCGATTTTACGCATACTg                                                                                                 <  1:296892/250‑1 (MQ=255)
                                                                                                                                                 gCCCTAAGCCGCCGGAATATTTACAGATTTTATAGCTGTCGAGCAGTTGACATTGCTCGCCCATTTTGACGTAGAGATCGAGCGTACGTTCTTCCTTGAAGATCTGGATGTAGACAGGGGATCCCATCAACTGCTGCTTATACTCTTTGCTCACAGGCGTGGTAGAGCTACTGCTGCCCAGCAAACCGGCAAATGAAACGCACGGGATCAACAACATCGCTAGAATTAATGCGATTGTACGCATACTGCtt                                                                                              <  1:160846/251‑1 (MQ=255)
                                                                                                                                                      aaGCCGCCGGAATATTTACAGATTGTATAGCTGTCGAGCAGTTGATATTGCTCGCCCATTTTGACGTAGAGATCGAGCGTACGTTCTTCCTTGAAGAGCTTGATGTAGACAGGGGATCCCATCAACTGCTGCTTATACTCTTTGCTCACAGTCGTGGTAGATCTACTGCTGCCCAGCAAACCGGCAAATGAAACGCACGGGATCAACATCATCGCAAGAATTAAGGAGATTTTACGCATACTGCTTAtt                                                                                           <  2:491749/249‑1 (MQ=255)
                                                                                                                                                         ccgccgGAATATTTACAGATTTTATAGCTGTCGAGCAGTTGATATTGCTCGCCCATTTTGACGTAGAGATCGAGCGTACGTTCTTCCTTGAAAATCTGGATGTAGACAGGGGATCCCATCAACTGCTGCTTATACTCTTTGCTCACAGGCGTGGTAGAGCTACTGCTGCCCAGCAAACCGGCAAATGAAACGCACGGGATCAACAACATCGCAAGAATTAATGCGATTTTACGCATACTGCTTATTCCTTg                                                                                      >  1:359703/1‑251 (MQ=255)
                                                                                                                                                              ggAATATTTACAGATGTTATAGCTGTCGAGCAGTTGATATTGCTCGCCCATTTTGACGTAGAGATCGAGCGTACGTTCTTCCTTGAAGATCTGGATGTAGACAGGGGATCCCATCAACTGCTGCTTATACTCTTTGCTCACAGGCGTGGTAGAGCTACTGCTGCCCAGCAAACCGGCAAATGAAACGCACGGGATCAACAACATCGCAAGAATTAATGCGATTTTACGCATACTGCTTATTCCTTGATaaa                                                                                 <  1:204864/251‑1 (MQ=255)
                                                                                                                                                                aaTATTTACAGATTTTATAGCTGTCGAGCAGTTGATATTGCTCGCCCATTTTGACGTAGAGATCGAGCGTACGTTCTTCCTTGAAAATCGGGATGTAGACAGGGGATCCCATCAACTGCTGCTTATACTCTTTGCTCACAGGCGTGGTAGAGCTACTGCTGCCCAGCAAACCGGCAAATGAAACGCACGGGATCAACAACATCGCAAGAATTAATGCGATTTTACGCATACTGCTTATTCCTTGATAAAAc                                                                               <  1:483112/251‑1 (MQ=255)
                                                                                                                                                                       aCAGATTTTATAGCTGTCGAGCAGTTGATATTGCTCGCCCATTTTGACGTAGAGATCGAGCGTACGTTCTTCCTTGAAGATCTGGATGTAGACAGGGGATCCCATCAACTGCTGCTTATACTCTTTGCTc                                                                                                                                                                                                 >  2:335054/1‑130 (MQ=255)
                                                                                                                                                                       aCAGATTTTATAGCTGTCGAGCAGTTGATATTGCTCGCCCATTTTGACGTAGAGATCGAGCGTACGTTCTTCCTTGAAGAGCTGGATGTAGACAGGGGATCCCATCAACTGCTGCTTATACTCTTTGCTc                                                                                                                                                                                                 <  1:335054/130‑1 (MQ=255)
                                                                                                                                                                       aCAGATTTTATAGCTGTCGAGCAGTTGATATTGCTCGCCCATTTTGACGTAGAGATCGAGCGTACGTTCTTCCTTGAAAATCTGGATGTAGACAGGGGATCCCATCAACTGCTGCTTATACTCTTTGCTCACAGGCGTGGTAGAGCTACTGCTGCCCAGCAAACCGGCAAATGAAACGCACGGGATCAACAACATCGCAAGAATTAATGCGATTTTACGCATACTGCTTATTCCTTGATAAAACGGTTac                                                                         >  1:27874/1‑250 (MQ=255)
                                                                                                                                                                         aGATTTTATAGCTGTAGAGCAGTTGATATTGCTCGCCCCTTTTGACGTAGAGATCGAGAGTACGTTCTTCCTTGAAGATCTGGATGTAGACAGGGGATCCCATCAACTGCTGCTTATACTCTTTGCTCACAGGCGTGGTAGAGCTACTGCTGCCCAGCAAACCGGCAAATGAAACGCACGGGATCAACAACCTCGCAAGAATTAATGCGATTTTACGCATACTGCTTATTCCTTGATAAAACGGTTacac                                                                       >  2:81734/1‑250 (MQ=255)
                                                                                                                                                                          gATTTTATAGCTGTCGAGCAGTTGATATTGCTCGCCCATTTTGACGTAGAGATCGAGCGTACGTTCTTCCGTGAAAATCTGGATGTAGACAGGTGATCCCATCAACTGCTGCTTATACTCTGTGCGCTCAGGCGTTGTAGAGCTACTGCTTTCCATCAATCCGGCAAATGGAACGCACTGGATCAACAACATCGCAAGAATTAATTCGATTTTACGCATACTGCTTATTCCTTGATAAAACGGTTacacac                                                                     <  1:494029/251‑1 (MQ=255)
                                                                                                                                                                                  agcggTCGAGCAGTTGATATTGCTCGCCCATTTTGACGTAGAGATCGAGCGTACGTTCTTCCTTGAAAATCTGGATGTAGACAGGGGATCCCATCAACTGCTGCTTATACTCTTTGCTCACAGGCGGGGTAGAGCTACTGCTGCCCAGCAAACCGGCAAATGAAACGCACGGGATCAACAACATCGCAAGAATTAATGCGATTTTACGCATACTGCTTATTCCTTGATAAAACGGTTACACACGCCAGGAc                                                             <  1:486718/247‑1 (MQ=255)
                                                                                                                                                                                  aGCTGTCGAGCAGTTGATATTGCTCGCCCATTTTGACGTAGAGATCGAGCGTACGTTCTTCCTTGAAAATCTGGATGTAGACAGGGGATCCCATCAACTGCTGCTTATACTCTTTGCTCACAGGCGTGGTAGAGCTACTGCTGCCCAGCAAACCGGCAAATGAAACGCACGGGATCAACAACATCGCAAGAATTAATGCTATTTTACGCATACTGCTTATTCCCTGATAAAACGGTTACACACGCCAGGAc                                                             <  1:131801/251‑1 (MQ=255)
                                                                                                                                                                                         gAGCAGTTGATATTGCTCGCCAATTTTGACGTAGAGATCGAGCGTACGTTCTTCCTTGAAAATCTGGATGTAGACAGGGGATCCCATCAACTGCTGCTTATACTCTTTGCTCACAGGCGTGGTAGAGCTACTGCTGCCCAGCAAACCGGCAAATGAAACGCACGGGATCAACAACATCGCAAGAATTAATGCGATTTTACGCATACTGCTTATTCCTTGATAAAACGGTTACACACGCCAGGACGGCaaa                                                       >  2:469583/1‑250 (MQ=255)
                                                                                                                                                                                            cAGTTGATATTGCTCGCCCATTTTGACGTAGAGATCGAGCGTACGTTCTTCCTTGAAAATCTGGATGTAGACAGGGGATCCCATCAACTGCTGCTTATACTCTTTGCTCACAGGCGTGGTAGAGCTACTGCTGCCCAGCAAACCGGCAAATGAAACGCACGGGATCAACAACATCGCAAGAATTAATGCGATTTTACGCATACTGCTTATTCCTTGATAAAACGGTTACACACGCCAGGACGGCAAAATgg                                                   >  1:308450/1‑251 (MQ=255)
                                                                                                                                                                                                           gCCCATTTTGACGTAGAGATCGAGCGTACGTTCTTCCTTGAAGATCTGGATGTAGACAGGGGATCCCATCAACTGCTGCTTATACTCGTTGCTCACAGGCGTGGTGGAGCTACTGCTGCCCAGCAAACCGGCAAATGAAACGCACGGGATCAACAACATCGCAATAATTAATGCGAGGTTACGCATACTGCTTATTCCTTGATAAAACGGTTACACACGCCAGGACGGCAAAATGGATCCCAAATCGGAAt                                    <  1:75439/251‑1 (MQ=255)
                                                                                                                                                                                                             ccATTTTGACGTAGAGATCGAGCGTACGTTCTTCCTTGAAGATCTGGATGTAGACAGGGGATCCCATCAACTGCTGCTTATACTCTTTGCTCACAGGCGTGGTAGAGCTACTGCTGCCCAGCAAACCGGCAAATGAAACGCACGGGATCAACAACATCGCAAGAATTAATGCGATTTTACGCATACTGCTTATTCCTTGATAAACCGGTTACACACGACAGGACGGCAAAATGGATCCCAAATCGGAAt                                    >  1:297571/1‑249 (MQ=255)
                                                                                                                                                                                                                         agagATCGAGCGTACGTTCTTCCTTGAAGATCTGGATGTAGACAGGGGATCCCATCAACTGCTGCTTATACTCTTTGCTCACAGGCGTGGTAGAGCTACTGCTGCCCAGCAAACCGGCAAATGAAACGCACGGGATCAACAACATCGCAAGAATTAATGCGATTTTACGCATACTGCTTATTCCTTGATAAAACGGTTACACACGCCAGGACGGCAAAATGGATCCCAAATCGGAATAGTCTGGATTTgg                       >  1:456415/1‑250 (MQ=255)
                                                                                                                                                                                                                         agagATCGAGCGTACGTTCTTCCTTGAAAATCTGGATGTAGACAGGGGATCCCATCAACTGCTGCTTATACTCTTTGCTCACAGGCGTGGTAGAGCTACTGCTGCCCAGCAAACCGGCAAATGAAACGCACGGGATCAACAACATCGCAAGAATTAATGCGATTTTACGCATACTGCTTATTCCTTGATAAAACGGTTACACACGCCAGGACGGCAAAATGGATCCCAAATCGGAATAGTCTGGATTTGGa                      <  1:83481/251‑1 (MQ=255)
                                                                                                                                                                                                                               cGAGCGTACGTTCTTACTTGAAAATGTGGATGTAGACAGGGGATCACATCAACTGCTGCTTATACTATTTGCTCACAGGCGTGGTAGAGCTACTGCTGCCCAGCAAACCGGCAAATGAAACGCAAGGGATCAACAACATCGCAATCATTAATGCGATTTTACGCATACTGCTTATTCCTTGATAAAACGGTTACAAACGCAAGGACGGCAAAATGGATCCCAAATCGGAATAGTCTGGATTTGTAAGGCTc                >  2:260250/1‑251 (MQ=255)
                                                                                                                                                                                                                                       cGTTCTTCCTTGAAGATCTGGATGTAGACAGGGGATCCCATCAACTGCTGCTTATACTCTTTGCTCACAGGCGTGGTAGAGCTACTGCTGCCCAGCAAACCGGCAAATGAAACGCACGGGATCAACAACATCGCAAGAATTAATGCGATTTTACGCATACTGCTTATTCCTTGATAAAACGGTTACACACGCCAGGACGGCAAAATGGATCCCAAATCGGAATAGTCTGGATTTGGAAGGCTCACATTATc        <  1:72487/251‑1 (MQ=255)
                                                                                                                                                                                                                                         ttcttcCTTGAAGATCTGGATGTAGACAGGGGATCCCATCAACTGCTGCTTATACTCTTTGCTCACAGGCGTGGTAGAGCTACTGCTGCCCAGCAAACCGGCAAATGAAACGCACGGGATCAACAACATCGCAAGAATTAATGCGATTTTACGCATACTGCTTATTCCTTGATAAAACGGTTACACACGCCAGGACGGCAAAATGGATCCCAAATCGGAATAGTCTGGATTTGGAAGGCTCACATTATCAc      >  2:134476/1‑251 (MQ=255)
                                                                                                                                                                                                                                               cTTGAAGATCTGGATGTAGAAAGGGGATCCCATCCACTGCTGCTTATACTCTTTGCTCACAGGCGTGGTAGAGCTGCTGCTGCCCCGAAAACCGGCAAATGAAACGCACGGGATAAACAGAATCGAAAGCATTAATGCGATTGTACGCATACTGCTTATTCCTTGATAAAACGGTTACACACGCCAGGCCGGCAAACTGGATCCCAAATCGGAATAGTCTGGATTTGGAAGGCTCCCAttctaacaaac  >  1:287947/1‑240 (MQ=255)
                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                  
TTCCCTTCATAACCATGCGCACGGTCATAGGCATTGGGGAAACCAATATTAATCGCTTTGTAGTAACGGCTGTCTGGTTTTAACTGATTACGCTGGACGCTATAAAACCCTTCCGGGCTTTTGAAATCGCCCTGACGCTGTTTTGGCCCTAAGCCGCCGGAATATTTACAGATTTTATAGCTGTCGAGCAGTTGATATTGCTCGCCCATTTTGACGTAGAGATCGAGCGTACGTTCTTCCTTGAAGATCTGGATGTAGACAGGGGATCCCATCAACTGCTGCTTATACTCTTTGCTCACAGGCGTGGTAGAGCTACTGCTGCCCAGCAAACCGGCAAATGAAACGCACGGGATCAACAACATCGCAAGAATTAATGCGATTTTACGCATACTGCTTATTCCTTGATAAAACGGTTACACACGCCAGGACGGCAAAATGGATCCCAAATCGGAATAGTCTGGATTTGGAAGGCTCACATTATCACCAAA  >  NC_000913/245417‑245904

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑