| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 244,775 | 0 | G | A | 50.7% | 5.2 / 107.7 | 76 | G150D (GGC→GAC) | yafJ | putative glutamine amidotransferase YafJ |
| Reads supporting (aligned to +/- strand): ref base G (23/14); new base A (20/18); total (44/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.86e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.63e-01 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
ATCCTGTTCGGTGGTGGCTCATATTCGCCAGGCTAATCGGGGCGAGGTGGCGCTGGAAAATACTCACCCATTTACCCGCGAGTTATGGGGGCGTAACTGGACTTATGCCCATAACGGACAACTGACGGGCTACAAATCACTGGAAACCGGCAACTTCCGCCCGGTAGGCGAAACCGACAGCGAAAAAGCCTTCTGCTGGCTCCTGCATAAATTAACGCAGCGTTACCCGCGCACACCGGGCAACATGGCGGCGGTATTTAAATATATCGCCTCACTGGCGGATGAACTGCGGCAGAAGGGCGTTTTCAACATGCTG‑CTTTCGGACGGGCGCTATGTAATGGCGTATTGCTCGACTAATTTACACTGGATCACCCGCCGCGCGCCGTTTGGCGTGGCAACGTTGCTGGATCAGGATGTGGAAATCGACTTCAGCTCGCAGACCACACCGAATGATGTGGTCACGGTGATTGCAACACAGCCGC > NC_000913/244536‑245017 | aTCCTGTTCGGTGGTGGCTCATATTCGCCAGGCTAATAGGGGCGAGGTGGCGCTGACAAATCCTCACCCATTTACCCGCGAGTTATGGGGGCGTAACTGGACTTATGCCCATAACGGACAACTGACGGGCTACAAATCACTGGAAACCGGCAACTTCCGCCCGGGCGGCGAAACCGACAGCGAAAAAGCCTTCTGCTGGCTCCTGCATAAATTAACGCAGCGTTACCCGCGCACACCGGGCAACATggcgg > 2:300362/1‑251 (MQ=255)aTCCTGTGCGGTGGTGGCTCATAGTCGCCAGGCTAATCGGGGCGAGGTGGCGCGGGCAAATACTCACCCATTTCCCCGCGAGTTATGGGGGCGTAACTGGACTTATGCCCATAACGGACAACTGACGGGCTACAAATCACTGGAAACCGGCAACTTCCGCCCGGTAGGCGAAACCGACAGCGAAAAAGCCTTCTGCTGGCTCCTGCATAAATTAACCCAGCGTTACCCGCGCACACCGGACAACATggcgg < 2:66667/251‑1 (MQ=255) tGTTCGGTGGAGGCTCATATTCGCCAGGCTAATCGGGGCGAGGTGGCGCTGGAAAATACTCACCCATTTACCCGAGAGTTATGGGGGCGTAACTGGACTTATGCCCATAACGGACAACTGACGGGCTACAAATCACTGGAAACCGGCAACTTCCGCCCGGTAGGCGAAACCGACAGCGAAAAAGCCTTCTGCTGGCTCCTGCATAAATTAACGCAGCGTTACCCGCGCACACCGGGCAACATGGcggcggg > 2:226281/1‑250 (MQ=255) ggtggCTCATATTCGCCAGGCTAATCGGGGCGAGGTGGCGCTGGAAAATACTCACCCATTTACCCGCGAGTTATGGGGGCGTAACTGGACTTATGCCCATAACGGACAACTGACGGGCTACAAATCACTGGAAACCGGCAACTTCCGCCCGGTAGGCGAAACCGACAGCGAAAAAGCCTTCTGCTGGCTCCTGCATAAATTAACGCAGCGTTACCCGCGCACACCGGACAACATGGCGGCGGTATTTAAat < 1:426337/251‑1 (MQ=255) tCATATTCGCCAGGCTAATCGGGTCGAGGTGGCGCTGGAATATACTCACCCATTTACCCGCGAGTTATGGGGGCGTAACTGGACTTATGCCCATAACGGACAACTGACGGGCTACAAATCACTGGACACCGGCAACATCCGCCCGGTAGGCGAAACCGACAGCGAACAAGCCTTCTGCTGGCTCCTGCATAAATTAACGCAGCGTTACCCGCGCACACCGGACAACATGGCGGCCGTATTTaaaagtatcg > 2:85776/1‑244 (MQ=255) atTCGCCAGGCTAATCGGGGCGAGGTGGCGCTGGAAAATACTCACCCATTTACCCGCGAGTTATGGGGGCGTAACTGGACTTATGCCCATAACGGACAACTGACGGGCTACAAATCACTGGAAACCGGCAACTTCCGCCCGGTAGGCGAAACCGACAGCGAAAAAGCCTTCTGCTGGCTCCTGCATAAATTAACGCAGCGTTACCCGCGCACACCGGACAACATGGCGGCGGTATTTAAATATATCGCCTc < 2:185342/251‑1 (MQ=255) ttCGCCAGGCTAATCGGGGCGAGGTGGCGCTGGAAAATACTCACCCATTTACCCGCGAGTTATGGGGGCGTAACTGGACTTATGCCCATAACGGACAACTGACAGGCTACAACTCACTGGAAACCGGCAACTTCCGCCCGGTAGGCGAAACCGACAGCGAAAAAGCCTTCTGCTGGCTCCTGCATAAATTAACGCAGCGTTACCCGCGCACACCGGACAACATGGCGGCGGTATTTAAATATATCGCCTc > 1:254698/1‑250 (MQ=255) gCCAGGCTAATCGGGGCGAGGTGGCGCTGGAAAATACTCACCCATTTACCCGCGAGTTATGGGGGCGTAACTGGACTTATGCCCATAACGGACAACTGACGGGCTACAAATCACTGGAAACCGGCAACTTCCGCCCGGTAGGCGAAACCGACAGCGAAAAAGCCTTCTGCTGGCTCCTGCATAAATTAACGCAGCGTTACCCGCGCACACCGGGCAACATGGCGGCGGTATTTAAATATATCGCCTCACTg < 1:464177/251‑1 (MQ=255) cTAATCGGGGCGAGGTGGCGCTGGAAAATACTCACCCCTTTACCCGCGAGTTATGGGGGCGTAACTGGACTTATGCCCATAACGGACAACTGACGGGCTACAAATCACTGGAAACCGGCAACTTCCGCCCGGTAGGCGAAACCGACAGCGAAAAAGCCTTCTGCTGGCTCCTGCATAAATTAACGCAGCGTTACCCCCGCACACCGGACAACATGGCGGCGGTATTTAAATATATAGCCTCACTGGCGGAt > 2:65219/1‑251 (MQ=255) aTCGGGGCGAGGTGTCGCTGGAAACTACTCCCCCATTTACCCGCGAGTTATGGGGGCGTAACTGGACTTATGCCCATAAAGGACAACTGACGGGCTACAAATCACTGGAAACCGGCAACTTCCGCCCGGTAGGCGAAACCGACAGCGAAAAAGCCTTCTGCTGGCTCCCGCATACATTAACGCACCGTTACACGCGCACACCGGGCAACATGGCGGCGGCATTTAAATCTATCGCCTCCCTAGCgggttt > 2:278874/1‑246 (MQ=255) ggggCGAGGTGGCGCTGGAAAATACTCACCCATTTACCCGCGAGTTATGGGGGCGTAACTGGACTTATGCCCATACCGGACAACTGACGGGCTACAAATCACTGGAAACCGGCAACTTCCGCCCGGTAGGCGAAACAGACAGCGAAAAAGCCTTCTGCTGGCTCCTGCATAAATTAACGCAGCGTTACCCGCGCACACCGGCCAACATGGCGGCGGTATTTAACTCTATCGCCTCACTGGCGGGTGGACTg > 2:284155/1‑251 (MQ=255) gggCGAGGTGGCGCTGGAAAATACTCACCCATTTACCCGCGAGTTATGGGGGCGTAACTGGACTTATGCCCATAACGGACAACTGACGGGCTACAAATCACTGGAAACCGGCAACTTCCGCCCGGTAGGCGAAACCGACAGCGAAAAAGCCTTCTGCTGGCTCCTGCATAAATTAACGCAGCGTTACCCGCGCACACCGGGCAACATGGCGGCGGTATTTAAATATATCGCCTCACTGGCGGATGAACTGc > 1:135587/1‑251 (MQ=255) cGAGGTGGCGCTGGAAAATACTCACCCATTTACCCGCGAGTTATGGGGGCGTAACTGGACTTATGCCCATAACGGACAACTGACGGGCTACAAATCACTGGAAACCGGCAACTTCCGCCCGGTAGGCGAAACCGACAGCGAAAAAGCCTTCTGCTGGCTCCTGCATAAATTAACGCAGCGTTACCCGCGCACACCGGACAACATGGCGGCGGTATTTAAATATATCGCCTCACTGGCGGATGAACTTCGGc > 1:486373/1‑251 (MQ=255) aGGTGGCGCTGGAAAATACTCACCCATTTACCCGCGAGTTATGGGGGCGTAACTGGACTTATGCCCATAACGGACAACTGACGGGCTACAAATCACTGGAAACCGGCAACTTACGCCCGGTAGGCGCAACCGACAGCGAAAAAGCCTTCTGCTGGCTCCTGCATAAATTAACGCAGCGTTACCCGCGCACACCGGACAACATGGCGGCGGTATTTACATATATAGCCTCACTGGCGGATGAACTGCGGcgg > 2:122580/1‑249 (MQ=255) ggCGCTGGAAAATACTCACCCATTTACCCGCGAGTTATGGGGGCGTACCTGGACTTATGCCCATAACGGACAACTGACGGGCTACAAATCACTGGAAACCGGCAACTTCCGCCCGGTAGGCGACACCGACAGCGAAAAAGCCTTCTGCTGGCTCCTGCATAAATTAACGCAGCGTTACCCGCGCACACCGGACAACATGGCGGCGGTATTTAAATATATCGCCTCACTGGCGGATGGACTGCGGCAGAAgg > 1:61511/1‑251 (MQ=255) cgcTGGAAAATACTCACCCATTTACCCGCGAGTTATGGGGGCGTACCTGGACTTATGCCCATAACGGACAACTGACGGGCTACAAATCACTGGAAACCGGCAACTTCCGCCCGGTAGGCGAAACCGACAGCGAAAAAGCCTTCTGCTGGCTCCTGCATAAATTAACGCAGCGTTACCCGCGCACACCGGACAACATGGCGGCGGTCTTTAACTATATCGCCTCACTGGCGGATGAACTGCGGCAGAAgggg > 1:302440/1‑250 (MQ=255) gcTGGAAAATACTCACCCATTTACCCGCGAGTTATGGGGGCGTAACTGGACTTATGCCCATAACGGACAACTGACGGGCTACAAATCACTGGAAACCGGCAACTTCCGCCCGGTAGGCGAAACCGACAGCGAAAAAGCCTTCTGCTGGCTCCTGCATAAATTAACGCAGCGTTACCCGCGCACACCGGGCAACATGGCGGCGGGATTTAAATATATCGACTCACTGGCGGAAGAACTGCGGCAGAAGGGCg > 2:375335/1‑251 (MQ=255) tGGAAAATACTCACCCATTTACCCGCGAGTTATGGGGGCGTAACTGGACTTATGCCCATAACGGACAACTGACGGGCTACAAATCACTGGAAACCGGCAACTTCCGCCCGGTAGGCGAAACCGACAGCGAAAAAGCCTTCTGCTGGCTCCTGCATAAATTAACGCAGCGTTACCCGCGCACACCGGGCAACCTGGCGGCGGTATTTAAATATATCGCCTCAATGGCGGCTGAACGGCGGCAGAAGGGCGtt > 2:30018/1‑251 (MQ=255) tGGAAAATACACACCCATTCACCTGCGAGTTATGGGGGCGTAACTGGACTTATGCCCATACCGGACAACTGCCGGGCTACAAATCACTGGAAACCGGCAACTTCCGCCCGGTAGGCGAAACCGACAGCGAAAAAGCCTTCTGCTGGCTCCTGCATAAATTAACGCAGCGTTACCCGCGCACACCGGGCAACATGGCGGCGGTATTTAAATATATCGCCTCACTGGCGGATGAACTGCGGCAGAAGGGCGtt > 2:308593/1‑251 (MQ=255) gAAAATACTCACCCATTTACCCGCGAGTTATGGGGGCGTAACTGGACTTATGCCCATAACGGACAACTGACGGGCTACAAATCACTGGAAACCGGCAACTTCCGCCCGGTAGGCGAAACCGACAGCGAAAAAGCCTTCTGCTGGCTCCTGCATAAATTAACGCAGCGTTACCCGCGCACACCGGGCAACATGGCGGCGGTATTTAAATATATCGCCTCACTGGCGGATGAACTGCGGCAGAAGGGCGtttt < 1:369984/251‑1 (MQ=255) aCTCACCCATTTACCCGCGAGTTATGGGGGCGTAACTGGACTTATGCCCATAACGGACAACTGACGGGCTACAAATCACTGGAAACCGGCAACTTCCGCCCGGTAGGCGAAACCGACAGCGAAAAAGCCTTCTGCTGGCTCCTGCATAAATTAACGCAGCGTTACCCGCGCACACCGGGCAACATGGCGGCGGTATTTAAATATATCGCCTCACTGGCGGATGAACTGCGGCAGAAGGGCGTTTTCAACa < 1:195796/250‑1 (MQ=255) aCTCACCCATTTACCCGCGAGTTATGGGGGCGTAACTGGACTTATGCCCATAACGGACAACTGACGGGCTACAAATCACTGGAAACCGGCAACTTCCGCCCGGTAGGCGAAACCGACAGCGAAAAAGCCTTCTGCTGGCTCCTGCATAAATTAACGCAGCGTTACCCGCGCACACCGGACAACATGGCGGCGGTATTTAAATATATCGCCTCACTGGCGGATGAACTGCGGCAGAAGGGCGTTTTCAACAt < 1:243970/251‑1 (MQ=255) ccGCGAGTTATGGGGGCGTAACTGGACTTATGCCCATAACGGACAACTGACGGACTACAAATCACTGGAAACCGGCAACTTCCGCCCGGTAGGCGAAACCGACAGCGAAAAAGCCTTCTGCTTGCTCCTGCATAAATTAACGCAGCGTTACCCGCGCACACCGGACAACATGGCGGCGGTATTTAAATATATCACCTCACTGGCGGATGAACTGCTGCATAAAGGCGTTTTCAACCTGCTG‑CTTTCGGACg > 1:1327/1‑251 (MQ=255) gcgAGTTATGGGGGCGTAACTGGACTTATGCCCATAACGGACAACTGACGGGCTACAAATCACTGGAAACCGGCAACTTCCGCCCGGTAGGCGAAACCGACAGCGAAAAAGCCTTCTGCTGGCTCCTGCATAAATTAACGCAGCGTTACCCGCGCACACCGGACAACATGGCGGCGGTATTTAACTATATCGCCTCACTGGCGGATGAACTGGGGCAGAAAGGGGGTTTCAAAATGGTGGCTTTCG‑ACggg > 2:377987/1‑251 (MQ=255) cgAGTTATGGACGCGTAATTGGCCTTCTGCCAATACCGGTCACCTGATGGGCTACAAATCACTGGAAACCGGCCAATCCCGACCGGTAGGCGACCCCGCAAGCGAAAAAGCCTTCTGCTGGCCACTGCATAAATTAACGCAGAGTTACCCACGCACACCGGACAACATGGCGGCGGTATTTAAATATATCGCCTCACTGGCGGATGAACCAGGGCAGGAGGGCGTTTTCAACCTACTG‑CTTTCGGCCgggg > 2:445913/1‑250 (MQ=255) taggggggcagaaCTGGACGTATGCCCATAACGGACAACTGACTGGCTACAAATCACTGGAAACCGGCAACTTCCGCCCGGTAGGCGAAACCGACAGCGAAAAAGCCTTCTGCTGGCTCCTGCATAAATTAACGCAGCGTTACCCGCGCACACCGGACAACATGGCGGCTGTAGTTAAAGATATCGCCTCACTGGCGGATGAACTTCGGCAGAAGGGCGTTGTCAACATGCTG‑CTTTCGGACGGGCGCTa < 2:61511/239‑1 (MQ=255) atgtgggCGTAACTGGACTTATGCCCATAACGGACAACTGACGGGCTACAAATCACTGGAAACCGGCAACTTCCGCCCGGTAGGCGAAACCGACAGCGAAAAAGCCTTCTGCTGGCTCCTGCATAAATTAACGCAGCGTTACCCGCGCACACCGGGCAACATGGCGGCGGTATTTAAATATATCGCCTCACTGTCGGATGAACTGCGGCAGAAGGGCGTTTGCAACATGCTG‑CTTTCGGACGGGCGCTATg < 1:387303/247‑1 (MQ=255) gggggCGTAACTGGACTTATGCCCATAACGGACAACTGACGGGCTACAAATCACTGGAAACCGGCAACTTCCGCCCGGTAGGCGAAACCGACAGCGAAAAAGCCTTCTGCTGGCTCCTGCATAAATTAACGCAGCGTTACCCGCGCACACCGGACAACATGGCGGCGGTATTTAAATATATCGCCTCACTGGCGGa > 2:129684/1‑196 (MQ=255) gggggCGTAACTGGACTTATGCCCATAACGGACAACTGACGGGCTACAAATCACTGGAAACCGGCAACTTCCGCCCGGTAGGCGAAACCGACAGCGAAAAAGCCTTCTGCTGGCTCCTGCATAAATTAACGCAGCGTTACCCGCGCACACCGGACAACATGGCGGCGGTATTTAAATATATCGCCTCACTGGCGGa < 1:129684/196‑1 (MQ=255) cGTAACTGGACTTATGCCCATAACGGACAACTGACGGGCTACAAATCACTGGAAACCGGCAACTTCAGCCCGGTAGGCGAAACCGACAGCGAAAAAGCCTTCTGCTGGCTCCTGCATAAATTAACGCAGCGTTACCCGCGCACACCGGACAACATGGCGGCGGTATTTAAATATATCGCCTCACTGGCGGATGAACTGCTGCCGAAGGGCGTTTTCAACATGGTG‑CTTTCGGGCGGGCGCTATGTAATGGc > 2:91706/1‑251 (MQ=255) aaCTGGACTTATGCCCATAACGGACAACTGACGGGCTACAAATCACTGGAAACCGGCAACTTCCGCCCGGTAGGCGAAACCGACAGCGAAAAAGCCTTCTGCTGGCTCCTGCATAAATTAACGCAGCGTTACCCGCGCACACCGGACAACATGGCGGCGGTATTTAAATATATCGCCTCACTGGCGGATGAACTGCGGCAGAAGGGCGTTTTCAACATGCTG‑CTTTCGGACGGGCGCTATGTAATGGCGt < 2:286574/250‑1 (MQ=255) tATGCCCATAACGGACAACTGACGGGCTACAAATCACTGGAAACCGGCAACTTCCGCCCGGTAGGCGAAACCGACAGCGAAAAAGCCTTCTGCTGGCTCCTGCATAAATTAACGCAGCGTTACCCGCGCACACCGGGCAACATGGCGGCGGTATTTAAATATATCGCCTCACTGGCGGATGAACTGCGGCAGAAGGGCGTTTTCAACATGCTG‑CTTTCGGACGGGCGCTATGTAATGGCGTATTGCTCGAc > 1:206036/1‑251 (MQ=255) aTGCCCATAACGGCCAACTGACGGGCTACAAATCACTGGAAACCGGCAACTTCCGCCCGGTAGGCGAAACCGACAGCGAACAAGCCTTCTGCTGGCTCCTGCATAAATTAACGCAGCGTTACCCGCGCACACCGGGCAACATGGCGGCGGTATTTAAATATATCGCCTCACTGGCGGATGAACTGCGGCAGAAGGGCGTTTTCAACATGCTG‑Cttt > 1:466276/1‑216 (MQ=255) aTGCCCATAACGGACAACTGACGGGCTACAAATCACTGGAAACCGGCAACTTCCGCCCGGTAGGCGAAACCGACAGCGAAAAAGCCTTCTGCTGGCTCCTGCATAAATTAACGCAGCGTTACCCGCGCACACCGGGCAACATGGCGGCGGTATTTAAATATACCGCCTCACTGGCGGATGAACTGCGGCAGAAGGGCGTTTTCAACATGCGG‑Cttt < 2:466276/216‑1 (MQ=255) gCCCATAACGGACAACTGACGGGCTACAAATCACTGGAAACCGGCAACTTCCGCCCGGTAGGCGAAACCGACAGCGAAAAAGCCTTCTGCTGGCTCCTGCATAAATTAACGCAGCGTTACCCGCGCACACCGGACAACATGGCGGCGGTATTTAAATATATCGCCTCACTGGCGGATGAACTGCGGCAGAAGGGCGTTTTCAACATGCTG‑CTTTCGGACGGGCGCTATGTAATGGCGTATTGCTCGACTa < 2:113592/250‑1 (MQ=255) ccATAACGGACATCTGACGGGCTACAAATCACTGGAAACCGGCAACTTCCGCCCGGTAGGCGAAACCGACAGCGAAAAAGCCTTCTGCTGGCTCCTGCATAAATTAACGCAGCGTTACCCGCGCACACCGGGCAACATGGCGGCGGTATTTAAATATATCGCCTCACTGGCGGATGAACTGCGGCAGAAGGGCGTTTTCAACATGCTG‑CTTTCGGACGGGCGCTATGTAATGGCGTATTGCTCGACTAAtt < 1:328838/251‑1 (MQ=255) ccATAACGGACAACTGACGGGCTACAAATCACTGGAAACCGGCAACTTCCGCCCGGTAGGCGAAACCGACAGCGAAAAAGCCTTCTGCTGGCTCCTGCATAAATTAACGCAGCGTTACCCGCGCACACCGGACAACATGGCGGCGGTATTTAAATATATCGCCTCACTGGCGGATGAACTGCGGCAGAAGGGCGTTTTCAACATGCTG‑CTTTCGGACGGGCGCTATGTAATGGCGTATTGCTCGACTAAtt < 2:238553/251‑1 (MQ=255) aaCGGCCAACTGACGGGCTACAAATCACTGGAAACCGGCAACTTCCGCCCGGTAGGCGAAACCGACAGCGAAAAAGCCTTCTGCTGGCTCCTGCATAAATTAACGCAGCGTTACCCGCGCACACCGGACAACATGGCGGCGGTATTTAAATATATCGCCTCACTGGCGGATGAACTGCGGCAGAAGGGCGTTTTCAACATGCTG‑CTTTCGGACGGGCGATATGTAATGGCGTATTGCTCGACTAATTTaca < 2:140211/251‑1 (MQ=255) cGGACAACTGACGGGCTACAACTCACTGGAAACCGGCACCTTCCGCCCGGTAGGCGAAACCGACAGCGAAAAAGCCTTCTGCTGGCTCCTGCATAAATTAACGCAGCGTTACCCGCGCACACCGGGCAACATGGCGGCGGTATTTAAATATATCGCCTCACTGGCGGATGGACTGCGGCCGAAGGGCGTTTTCAACATGCTG‑CTTTTGGGCGGGCGCTCTGTAATGGCGGTTTTCTCGACACATTTacacg > 2:160875/1‑250 (MQ=255) aCAACTGACGGGCTACAAATCACTGGAAACCGGCAACTTCCGCCCGGTAGGCGAAACCGACAGCGAAAAAGCCTTCTGCTGGCTCCTGCATAAATTAACGCAGCGTTACCCGCGCACACCGGACAACATGGCGGCGGTATTTAAATATATCGCCTCACTGGCGGATGAACTGCGGCAGAAGGGCGTTTTCAACATGCTG‑CTTTCGGACGGGCGCTATGTAATGGCGTATTGCt < 2:401922/233‑1 (MQ=255) aCAACTGACGGGCTACAAATCACTGGAAACCGGCAACTTCCGCCCGGTAGGCGAAACCGACAGCGAAAAAGCCTTCTGCTGGCTCCTGCATAAATTAACGCAGCGTTACCCGCGCACACCGGACAACATGGCGGCGGTATTTAAATATATCGCCTCACTGGCGGATGAACTGCGGCAGAAGGGCGTTTTCAACATGCTG‑CTTTCGGACGGGCGCTATGTAATGGCGTATTGCt > 1:401922/1‑233 (MQ=255) aCTGACGGGCTACAACTCACTGGAAACCGGCAACTTCCGCCCGGTAGGCGAAACCGACAGCGAAAAAGCCTTCTGCTGGCTCCTGCATAAATTAACGCAGCGTTACCCGCGCACACCGGGCAACATGGCGGCGGTAt > 2:111326/1‑137 (MQ=255) aCTGACGGGCTACAAATCACTGGAAACCGGCAACTTCCGCCCGGTAGGCGAAACCGACAGCGAAAAAGCCTTCTGCTGGCTCCTGCATAAATTAACGCAGCGTTACCCGCGCACACCGGGCAACATGGCGGCGGTAt < 1:111326/137‑1 (MQ=255) gACGGGCTACAAATCACTGGAAACCGGCAACTTCCGCCCGGTAGGCGAAACCGACAGCGAAAAAGCCTTCTGCTGGCTCCTGCATAAATTAACGCAGCGTTACCCGCGCACACCGGGCAACATGGCGGCGGTATTTAAATATATCGCCTCACTGGCGGATGAACTGCGGCAGAAGGGCGTTTTCAACATGCTG‑CCTTCGGACGGGCGCTATGTAATGGCGTATTGCTCGACTAATTTACACTGGATCAccc < 2:121040/251‑1 (MQ=255) aCGGGCTACAAATCACTGGAAACCGGCAACTTCCGCCCGGTAGGCGAAACCGACAGCGAAAAAGCCTTCTGCTGGCTCCTGCATAAATTAACGCAGCGTTACCCGCGCACACCGGGCAACATGGCGGCGGTATTTAAATATATCGCCTCACTGGCGGATGAACTGCGGCAGAAGGGCGTTTTCAACATGCTG‑CTTTCGGACGGGCGCTATGTAATGGCGTATTGCTCGACTAATTTACACTGGATCACccg < 2:209403/251‑1 (MQ=255) cGGGCTACAAATCACTGGAAACCGGCAACTTCCGCCCGGTAGGCGAAACCGACAGCGAAAAAGCCTTCTGCTGGCTCCTGCATAAATTAACGCAGCGTTACCCGCGCACACCGGACAACATGGCGGCGGTATTTAAATATATCGCCTCACTGGCGGATGAACTGCGGCAGAAGGGCGTTTTCAACATGCTG‑CTTTCGGACGGGCGCTATGTAATGGCGTATTGCTCGACTAATTTACACTGGATCACccgc < 2:113493/251‑1 (MQ=255) ggCTACAAATCACTGGAAACCGGCAACTCCCGCCCGGTAGGCGAAACCGACAGCGAAAAAGCCTTCTGCTGGCTCCTGCATAAATTAACGCAGCGTTACCCGCGCACACCGGGCATCATGGCGGCGGTATTTAAATATATCGCCTCATTGGCGGAGGAACGGCGGCAGAAGGGCGTTTGCAACATGCGG‑CTTTCGGACGGGCGCTATGTAATGGCGTATTGCTCGACTAATTTACACTGTATCACccgccg < 2:192690/251‑1 (MQ=255) tACAAATCAATGGAAACCGGCAACTTCCGCCCGGTAGGCGAAACCGACAGCGAAAAAGCCTTCTGCTGGCTCCTGCATAAATTAACGCAGCGTTACCCGCGCACACCGGGCAACATGGCGGCGGTATTTAAATATATCGCCTCACTGGCGGATGAACTGCGGCAGCAGGGCGTTTTCAACATGCTG‑CTTTCGGGCGGGCGCTATGTAATGGCGTATTGCTCGACTAATTTACACTGGATAAcccgccccgc > 1:415660/1‑251 (MQ=255) cAAATCACTGGAAACCGGCAACTTCCGCCCGGTAGGCGAAACCGACAGCGAAAAAGCCTTCTGCTGGCTCCTGCATAAATTAACGCAGCGTTACCCGCGCACACCGGGCAACATGGCGGCGGTATTTAAATATATCGCCTCACTGGCGGATGAACTGCGGCAGAAGGGCGTTTTCAACATGCGG‑CTTTTGGACGGGCGCTATGTAATGGCGGATTGCTCGACTAATTTACACTTGTTCACCCGccgcccgc > 2:362596/1‑251 (MQ=255) aaaTCACTGGCAACCGGAAACTTCCGCCCAATAGGCGCAACCGACAGCGAAAAAGCCTTATACTGGCTCCTGCATAAATTAACGCAGCGTTACCCGCGCACACCGGACAACATGGCGGAGGCATTTAAATATATCGCCTCACTGGCGGATGGACTGCGGCCGAAAGGCGTTTTCAACATGCTG‑CTTTCAGACGGGCGCTCTGTATAGGCGGATTTCTCGACTCATTTACACTGGCTCACACGCCCCGCGcc > 2:331224/1‑251 (MQ=255) cACTGGAAACCGGCAACTTCCGCCCGGTAGGCGAAACCGACAGCGAAAAAGCCTTCTGCTGGCTCCTGCATAAATTAACGCAGCGTTACCCGCGCACACCGGACAACATGGCGGCGGTATTTAAATATACCGCCTCACTGGCGGATGAACTGCGGCAGAAGGGCGTTTTCAACATGCTG‑CTTTCGGACGGGCGCTATGTAATGGCGTATTGCTCGACAAATTTACACTGGATCACCCGCCGCGCGCCggtt > 1:489330/1‑248 (MQ=255) gAAACCGGCACCTTAAGCCCGGTAGGCGAAACCGACAGCGAAAAAGCCTTATGCTGGCCCCTGCATAAATTAACGAAGCGTTACCCGCGCACACCGGACAACATGGCGGCGGTATTTAAATATATCGCCTCACTGGCGGGTGAACTGCGGCAGAAGGGCGTTTTCAACATGCTG‑CTTTCGGCCGGGCGCTATGTAATGGCGGATTGCTCGACTAATTTACACTGGGTTCCCCCCCGCGCCCCGTTTGGCgg > 2:210145/1‑250 (MQ=255) aaaCCGGCAACTTCCGCCCGGTAGGCGAAACCGACAGCGAAAAAGCCTTCTGCTGGCTCCTGCATAAATTAACGCAGCGTTACCCGCGCACACCGGACAACATGGCGGCGGTATTTAAATATATCGCCTCACTGGCGGATGAACTGCGGCAGAAGGGCGTTTTCAACATGCTG‑CTTTCGGACGGGCGCTATGTAATGGCGTATTGCTCGACTAATTTACACTGGATCACCCGCCGCGCGCCGTTTGGCGTg > 1:15121/1‑251 (MQ=255) ggCAACTTCCGCCCGGTAGGCGAAACCGACAGCGAAAAAGCCTTCTGCTGGCTCCTGCATAAATTAACGCAGCGTTACCCGCGCACACCGGACAACATGGCGGCGGTATTTAAATATATCGCCTCACTGGCGGATGAACTGCGGCAGAAGGGCGTGTTCAACATGCTG‑CTTTCGGACGGGCGCTATGTAATGGCGTATTGCTCGACTAATTTACACTGGATCACCCGCCGCGCGCCGTTTGGCGTGGCAAc < 1:284155/251‑1 (MQ=255) aaCTTCCGCCCGGTAGGCGAAACCGACAGCGAAACAGCCTTCTGCTGGCTCCTGCATAAATTAACGCAGCGTTACCCGCGCACACCGGGCAACCTGGCGGCGGTATTTAAATATATCGCCTCCCTGGCggc > 2:216643/1‑130 (MQ=255) aaCTTCCGCCCGGTAGGCGAAACCGACAGCGAAAAAGCCTTCTGCTGGCTCCTGCATAAATTAACGCAGCGTTACCCGCGCACACCGGGCAACATGGCGGCGGTATTTAAATATATCGCCTCACTGGCGGa < 1:216643/131‑1 (MQ=255) cTTCCGCCCGGTAGGCGAAACCGACAGCGAAAAAGCCTTCTGCTGGCTCCTGCATAAATTAACGCAGCGTTACCCGCGCACACCGGGCAACATGGCGGCGGTATTTAAATATATCGCCGCACTGGCGGATGAAATGCGGCCGGAGGGCGGTTTCAACATGCTG‑CTTTCGGCCGGGCGCTATGTAAAGGCGTAATTCTCGACTAATTTCAACTGGATCACCCGCCGAGCGCCGTTTGGCGTGGGAACGTTgt > 2:447566/1‑250 (MQ=255) ccctctAGGCGAACCCGACGGCGAAAAAGCCTTCTGCTGGCTCCTGCATAAATTAACTCAGCGTAACCCGCGCTCACCGGGCAACAGGGCGGCGGTATTGAAAGATATCTCCGCACGGGTGGATGAACTGCGGCAGAAGGGCTTTTTCAACAGTCTG‑CTGTCGGTGGGGCGCTTTGTATTGGCGTTTTTCTCGGCTGATTTCCACGTGATCATCCGCCGGCCGCTGTTTGGCGTgggcagggtgctggagc < 2:461300/246‑16 (MQ=255) aCCGACAGCGAAAAAGCCTTCTGCTGGCTCCTGCATAAATTAACGCAGCGTTACCCGCGCACACCGGGCAACATGGCGGCGGTATTTAAATATATCGCCTCACTGGCGGATGAACTGCGGCAGAAGGGCGTTTTCAACATGCTG‑CTTTCGGACGGGCGCTATGTAATGGCGTATTGCTCGACTAATTTACACTGGATCACCCGCCGCGCGCCGTTTGGCGTGGCAACGTTGCTGGATCAGGATGTGGAAAt < 2:126976/251‑1 (MQ=255) gACAGCGAAAAAGCCTTCTGCTGGCTCCTGCATAAATTAACGCAGCGTTACCCGCGCACACCGGGCAACATGGCGGCGGTATTTAAATATATCGCCTCACTGGCGGATGAACTGCGGCAGAAGGGCGTTTTCAACATGCTG‑CTTTCGGACGGGCGCTATGTAATGGCGTATTGCTCGACTAATTTACACTTGATCACCCGCCGCGCGCCGTTTGGCGTGGCAACGTTGCTGGCTCAGGATGTGGAAATCgc > 1:314635/1‑250 (MQ=255) aCAGCGAAAAAGCCTTCTGCTGGCTCCTGCATAAATTAACGCAGCGTTACCCGCGCACACCGGACAACATGGCGGCGGTATTTAAATATATCGCCTCACTGGCGGATGAACTGCGGCAGAAGGGCGTTTTCAACATGCTG‑CTTTCGGACGGGCGCTATGTAATGGCGTATTGCTCGACTAATTTACACTGGATCACCCGCCGCGCGCCGTTTGGCGTGGCAACGTTGCTGGATCAGGATGTGGAAATCGAc < 1:314846/251‑1 (MQ=255) cGAAAACGCCTTCTGCTGGCACCTGCCTAAATTACCGCAGCGTTACCCGCGCACACCGGACAACATGGCGGCGGTATTTAAATATATCGCCTCACTGGCGGATGAACTGCGGCAGAAGGGCGTTTTCAACCTGCTG‑CGTTCGGACCGGCGGTATGTAATGGCGTATTGCCCGCCTAATTTACACTGGATCACCCGCCGCGCGCCGTTTAGAGTGGCAACGTTGCTGGAACAGGGTGTGGTAATCGaattt > 2:89433/1‑246 (MQ=255) ttCTGCTGGCTCCTGCATAAATTAACGCAGCGTTACCCACGCACACCGGACAACATGGCGGCGGTATTTAAATATATCGACTAACTGGCGGATGAACTGTGGCAGAAGGGCGTTTTCAACATGCTG‑GTTTCGGACGGGCGCTCTGTAATGGCGTATTGCTCGACTAATTTACACTGGATCCCCCGCCGCCCGCCGTTTAGCGTGGCCACCGTGCTGGGCCAGGGTGGGGAAATCGACTTCCGCCCGCaaac > 2:197794/1‑248 (MQ=255) ctgctgGCTCCTGCATAAATTAACGCAGCGTTACCCGCGCACACCGGGCAACATGGCGGCGGTATTTAAATATATCGCCTCACTGGCGGATGAACTGCGGCAGAAGGGCGTTTTCAACATGCTG‑CTTTCGGACGGGCGCTATGTAATGGCGAATTGCTCGACCAATTTACACTGGATCCCCCGCCGCGCGCCGTTTTGCGTGGCAACGTTGCTGGACCAGGATGTGGAAATCGACTTCAGCTCGCAgtccc > 1:296806/1‑247 (MQ=255) tgGCTCCTGCATAAATTACCGCAGCGTTACCCGCGCACACCGGGCAACATGGCGGCGGTATTTAAATATATCGCCTCACTGGCGGATGAAATGCGGCAGAAGGGCGTTTTCAACATGCTG‑ATTTCGGACGGGCGCTATGTAATGGCGTATTGCTCGACTAATTTACACTGGATCCACCGCCGCGCGCCGTTTGGCGTGGCCACGTTGCTGGCTCAGGTTGGGGACATCGAATTCCGCTCGCAGACCCCAcc > 2:306490/1‑251 (MQ=255) gCTCCTGCATAAATTAACGCAGCGTTACCCGCGCACACCGGACAACATGGCGGCGGTATTTAAATATATCGCCTCACTGGCGGATGAACTGCGGCCGAAGGGCGTTTTCAACATGCTG‑CTTTCGGACGGGCGCTATGTAAAGGCGGATTGCTCGGCTAATTTACACTGGATCACCCGCCGCGCGCCGTTTGGCGTGGCAACGTTGCTGGATCAGGATGTGGGAATCGACTTCAGCTCGCAGACCCCACCg > 2:335625/1‑250 (MQ=255) tCCTGCATAAATTAACGCAGCGTTACCCGCGCACACCGGGCAACATGGCGGCGGTATTTAAATATATCGCCTCACTGGCGGATGAACTGCGGCAGAAGGGCGTTTTCAACATGCTG‑CTTTCGGACGGGCGCTATGTAATGGCGGATTGCTCGACTAATTTACACTGGATCACCCGCCGCGCGCCGTTTGGCGTGGCAACGTTGCTTGATCAGGATGGGGTAATCGACTTCAGCTCGCAGACCAAACCGAat > 1:155625/1‑251 (MQ=255) tAAATTAACGCAGCGTTACCCGCGCACACCGGGCAACATGGCGGCGGTATTTAAATATATCGCCTCACTGGCGGATGAACTGCGGCAGAAGGGCGTTTTCAACATGCTG‑CTTTCGGACGGGCGCTATGTAATGGCGTATTGCTCGACTAATTTACACTGGATCACCCGCCGCGCGCCGTTTGGCGTGGCAACGTTGCTGGATCAGGATGTGGAAATCGACTTCAGCTCGCAGACCACACCGAATGATGTgg > 1:205086/1‑251 (MQ=255) aaTTAACGCAGCGTTACCCGCGCACATCGGACAACATGGCGGCGGTATTTAAATATATCGCCTCACTGGCGGATGAACTGCGGCAGAAGGGCGTTTTCAACATGCTG‑CTTGCGGACGGTTGCTATGTAATTGCGTATTTCTCTACTAATTTACACTGGATCACCCGCCGCGCGCCGTTTGGTGTGTCAACGTTGCTGGGTCAGGATGTGGAAATCGACTTCAGCTCGCAGACCACACCGAATGATGTGGTc < 1:491354/251‑1 (MQ=255) aaTTAACGCAGCGTTACCCGCGCACACCGGACAACATGGCGGCGGTATTTAAATATATCGACTCACTGGCGGATGAACTGCGGCAGAAGGGCGTTTTCAACATGCTG‑CTTTCGGACGGGCGCTATGTAATGGCGTATTGCTCGACTAATTTACACTGGATCACCCGCCGCGCGCCGTTTGGCGTGGCAACGTTGCTGGATCAGGATGTGGAAATCGACTTCAGCTCGCAGACCACACCGAATGATGTGGTc < 1:372326/251‑1 (MQ=255) aaCGCAGCGTTACCCGCGCACACCGGGCAACATGGCGGCGGTATTTAAATATATCGCCTCACTGGCGGATGAACTGCGGCAGAAGGGCGTTTTCAACATGCTG‑CTTTCGGACGGGCGCTATGTAATGGCGTATTGCTCGACTAATTTACACTGGATCACCCGCCGCGCGCCGTTTGGCGTGGCAACGTTGCTGGATCAGGATGTGGAAATCGACTTCAGCTCGCAGACCACACCGAATGATGTGGTCACgg < 1:431015/251‑1 (MQ=255) gCAGCGTTACCCGCGCACACCGGACAACATGGCGGCGGTATATAAATATATCGCCTCACTGGCGGATGAACTGCGGCAGAAGGTCGTTTTCAACAGGCTG‑CTTTCGGACGGGCGCTATGAAATGGCGTATTGCTCGACTAATTTACACTGGATCACCCGCCGCGCGCCGGTTGGCGTGGCAACGTTGCTGGATCAGGATGTGGAAATCGACTTCAGCTCGCAGACCACACCGAATGATGTGGTCACGGTGa < 2:177988/251‑1 (MQ=255) cAGCGTTACCCGCGCACACCGGGCAACATGGCGGCGGTATTTAAATATATCGCCTCACTGGCGGATGAACTGCGGCAGAAGGGCGTTTTCAACATGCTG‑CTTTCGGACGGGCGCTATGTAATGGCGTATTGCTCGACTAATTTACACTGGATCACCCGCCGCGCGCCGTTTGGCGTGGCAACGTTGCTGGATCAGGATGTGGAAATCGACTTCAGCTCCCAGACCACACCGAATGATGTGGTCACGGTGAt > 1:17040/1‑251 (MQ=255) gTTACCCGCGCACACCGGGCAACATGGCGGCGGTATTTAAATATATCGCCTCACTGGCGGATGAACTGCGGCAGAAGGGCGTTTTCAACATGCTG‑CTTTCGGACGGGCGCTATGTAATGGCGTATTGCTCGACTAATTTACACTGGATCACCCGCCGCGCGCCGTTTGGCGTGGCAACGTTGCTGGATCAGGATGTGGAAATCGACTTCAGCTCGCAGACCACACCGAATGATGTGGTCACGGTGATTGc > 1:359061/1‑250 (MQ=255) ccGCGCACCCCGGACAACAGGGCGGCGGTATTTAAATATATCGCCTCACTGGCGGATGAACTGCGGCAGAAGGGCGTTTTCAACATGCTG‑CTTTCGGACGGGCGCTATGTAATGGCGTATTGCTCGACTAATTTACACTGGATCACCCGCCGCGCGCCGTTTGGCGTGGCAACGTTGCTGGATCAGGATGTGGAAATCGACTTCAGCTCGCAGACCACACCGAATGATGTGGTCACTGTGATTGCAAcaca < 2:304718/251‑1 (MQ=255) cacaCCGGGCAACATGGCGGCGGTATTTAAATATATCGCCTCACTGGCGGATGAACTGCGGCAGAAGGGCGTTTTCAACATGCTG‑CTTTCGGACGGGCGCTATGTAATGGCGTATTGCTCGACTAATTTACACTGGATCCCCCGCCGCGCGCCGTTTGGCGTGGCAACGTTGCTGGATCAGGATGTGGAAATCGACTTCAGCTCGCAGACCCCACCGAATGATGTGGTCACGGTGATTGCAACACAGCCGc > 2:166908/1‑251 (MQ=255) | ATCCTGTTCGGTGGTGGCTCATATTCGCCAGGCTAATCGGGGCGAGGTGGCGCTGGAAAATACTCACCCATTTACCCGCGAGTTATGGGGGCGTAACTGGACTTATGCCCATAACGGACAACTGACGGGCTACAAATCACTGGAAACCGGCAACTTCCGCCCGGTAGGCGAAACCGACAGCGAAAAAGCCTTCTGCTGGCTCCTGCATAAATTAACGCAGCGTTACCCGCGCACACCGGGCAACATGGCGGCGGTATTTAAATATATCGCCTCACTGGCGGATGAACTGCGGCAGAAGGGCGTTTTCAACATGCTG‑CTTTCGGACGGGCGCTATGTAATGGCGTATTGCTCGACTAATTTACACTGGATCACCCGCCGCGCGCCGTTTGGCGTGGCAACGTTGCTGGATCAGGATGTGGAAATCGACTTCAGCTCGCAGACCACACCGAATGATGTGGTCACGGTGATTGCAACACAGCCGC > NC_000913/244536‑245017 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |