Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,062,3200AC22.6% 223.7 / 11.9 125T491P (ACC→CCC) scpAmethylmalonyl‑CoA mutase
Reads supporting (aligned to +/- strand):  ref base A (69/27);  new base C (0/28);  total (69/56)
Fisher's exact test for biased strand distribution p-value = 7.68e-13
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 126 total aligned reads displayed.

AAGCCAGAGCTATTATCCAACAGATCGACGAAGCCGGTGGCATGGCGAAAGCGATCGAAGCAGGTCTGCCAAAACGAATGATCGAAGAGGCCTCAGCGCGCGAACAGTCGCTGATCGACCAGGG‑CAAGCGTGTCA‑TCGTTGGTGT‑CAACAA‑GT‑ACAAA‑CTGGATCACGAAGA‑CGAAACCGATG‑TACTTGAGA‑TCGACAACGTGATGGTGCGTAACGAGCAAATTGCTTC‑GCTGGAACGC‑ATTCGCGCCACCCGTGATGATGCCGCCGTAACCGCCGCGTTGAACGCCCTGACTCACGCCGCACAGCATAACGAAAACCTGCTGGCTGCCGCTGTTAATGCCGCTCGCGTTCGCGCCACCCTGGGTGAAATTTCCGATGCGCTGGAAGTCGCTTTCGACCGTTATCTGGTGCCAAGCCAGTGTGTTACCGGCGTGATTGCGCAAAGCTATCATCAGTCTGAGAAATCGGCCTCCGAGTTCGATGCCATTGTTGCGCA  >  NC_000913/3062072‑3062567
                                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                       
aaGCCAGCGCTATTATCCAACAGATCGACGAAGCCGGTGGCCTGGCGAAAGCGATCGAAGCAGGTCTGCCAAAACGAATGATCGAAGAGGCCTCAGCGCGCGAACAGTCGCTGATCGACCAGGG‑CACGCGTGTCA‑TCGTTGGTGT‑CAACAA‑GT‑ACAAA‑CTGGCTCACGAAGA‑CGAAACCGATG‑TACTTGAGC‑TCGACAACGTGATGGTGCGTAACGAGCAAATTGCTTC‑GCTGGAACGC‑ATTCGCGCCAcc                                                                                                                                                                                                                                                       >  2:205334/1‑251 (MQ=255)
aaGCCAGAGCTATTATCCAACAGATCGACGAAGCCGGTGGCATGGCGAAAGCGATCGAAGCAGGTCTGCCAAAACGAATGATCGAAGAGGCCTCAGCGCGCGAACAGTCGCTGATCGACCAGGG‑CAAGCGTGTCA‑TCGTTGGTGT‑CAACAA‑GT‑ACAAA‑CTGGATCACGAAGA‑CGAAACCGATG‑TACTTGAGA‑TCGACAACGTGATGGTGCGTAACGAGCAAATTGCTTC‑GCTGGAACGC‑ATTCGCGCCAcc                                                                                                                                                                                                                                                       >  1:319331/1‑251 (MQ=255)
aaGCCAGAGCTATTATCCAACAGATCGACGAAGCCGGTGGCATGGCGAAAGCGATCGAAGCAGGTCTGCCAAAACGAATGATCGAAGAGGCCTCAGCGCGCGAACAGTCGCTGATCGACCAGGG‑CAAGCGTGTCA‑TCGTTGGTGT‑CAACAA‑GT‑ACAAA‑CTGGATCACGAAGA‑CGAAACCGATG‑TACTTGAGA‑TCGACAACGTGATGGTGCGTAACGAGCAAATTGCTTC‑GCTGGAACGC‑ATTCGCGCCAcc                                                                                                                                                                                                                                                       <  2:212579/251‑1 (MQ=255)
  gCCAGAGCTATTATCCAACAGATCGACGAAGCCGGTGGCATGGCGAAAGCGATCGAAGCAGGTCTGCCAAAACGAATGATCGAAGAGGCCTCAGCGCGCGAACAGTCGCTGATCGACCAGGG‑CAAGCGTGTCA‑TCGTTGGTGT‑CAACAA‑GT‑ACAAA‑CTGGATCACGAAGA‑CGAAACCGATG‑TACTTGAGA‑TCGACAACGTGATGGTGCGTAACGAGCAAATTGCTTC‑GCTGGAACGC‑ATTCGCGCCACCCg                                                                                                                                                                                                                                                     <  2:299703/251‑1 (MQ=255)
  gCCAGAGCTATTATCCAACAGATCGACGAAGCCGGTGGCATGGCGAAAGCGATCGAAGCAGGTCTGCCAAAACGAATGATCGAAGAGGCCTCAGCGCGCGAACAGTCGCTGATCGACCAGGG‑CAAGCGTGTAA‑TCGTTGGTGT‑CAACAA‑GT‑ACAAC‑CTGGATCACGAAGA‑CGAAACCGATG‑GACTTGAGA‑TCGACAACGTGATGGTGCGTAACGAGCAAATTGATTC‑GCTGGAACGC‑ATTCGCGCCACCCg                                                                                                                                                                                                                                                     >  1:445378/1‑251 (MQ=255)
   tcAGACCTATTATCCAACAGATCGACGAAGCCGGTGGCATGGCGAAAGCGATCGACGCAGGCCTGCCAAAACGAATGATCGAAGAGGCCTCAGCGCGAGAACAGTCGCTGATCGAACAGGA‑CAAGCGTGTCA‑TCGTTGGTGT‑CAACAA‑GT‑ACATA‑CTGGATCACGAAGA‑CGAAACCGATG‑TACTTGAGA‑TCGACAACGTGAAGGTGCGCAACGTGCAGATTGCTTC‑GCTGGAACGC‑ATTCGCGCCACCCGt                                                                                                                                                                                                                                                    >  2:242381/2‑251 (MQ=255)
          tattatCCAACAGATCGACGAAGCCGGTGGCATGGCGAAAGCGATCGAAGCAGGTCTGCCAAAACGAATGATCGAAGAGGCCTCAGCGCGCGAACAGTCGCTGATCGACCAGGG‑CAAGCGTGTCA‑TCGTTGGTGT‑CAACAA‑GT‑ACCAA‑CTGGATCACGAAGA‑CGAAACCGATG‑TACTTGAGA‑TCGACAACGCGATGGTGCGTAACGAGCAAATTGCTCC‑GCTGGAACGC‑ATTAGCGCCACCCGTGatgatg                                                                                                                                                                                                                                             >  2:23561/1‑251 (MQ=255)
           attatCCAACAGATCGACGAAGCCGGTGGCATGGCGAAAGCGATCGAAGCAGGTCTGCCAAAACGAATGATCGAAGAGGCCTCAGCGCGCGAACAGTCGCTGATCGACCAGGG‑CAAGCGTGTCA‑TCGTTGGTGT‑CAACAA‑GT‑ACAAA‑CTGGATCACGAAGA‑CGAAACCGATG‑TACTTGAGA‑TCGACAACGTGATGGTGCGTAACGAGCAAATTGCTTC‑GCTGGAACGC‑ATTCGCGCCACCCGTGATGATgc                                                                                                                                                                                                                                            >  1:57715/1‑251 (MQ=255)
             tatCCAACAGATCGACGAAGCCGGTGGCATGGCGAAAGCGATCGAAGCAGGTCTGCCAAAACGAATGATCGAAGCGGCCTCAGCGCGCGAACAGTCGCTGATCGACCAGGG‑CAAGCGTGTCA‑TCGTTGGTGT‑CAACAA‑GT‑ACAAA‑CTGGATCACGAAGA‑CGAAACCGATG‑TACTTGAGA‑TCGACAACGTGATGGTGCGTAACGAGCAAATTGCTTC‑GCTGGAACGC‑ATTCGCGCCACCCGTGATGATgccg                                                                                                                                                                                                                                          >  1:473144/1‑251 (MQ=255)
             tatCCAACAGATCGACGAAGCCGGTGGCATGGCGAAAGCGATCGAAGCAGGTCTGCCAAAACGAATGATCGAAGAGGCCTCAGCGCGCGAACAGTCGCTGATCGACCAGGG‑CAAGCGTGTCA‑TCGTTGGTGT‑CAACAA‑GT‑ACAAA‑CTGGATCACGAAGA‑CGAAACCGATG‑TACTTGAGA‑TCGACAACGTGATGGTGCGTAACGAGCAAATTGCTTC‑GCTGGAACGC‑ATTCGCGCCACCCGTGATGATgcc                                                                                                                                                                                                                                           <  1:343199/250‑1 (MQ=255)
                      gATCGAAGAAGCCTGTGGCATGGCGAAAGCGACCGAAGATGGTCTGCCAAAACGAATGATCGAAGAGGCCGCAGCGCGCGAACAGTCGATGAGCGCCCAGGG‑CAAGCGTGTCA‑TCGTTGGTGT‑CAACAA‑GT‑ACAGA‑CTGGATCACGAAGA‑CGAAACCGATG‑TACTTGAGA‑TCGACAACGTGATGGTGCGTAACGAGCAAATGGCTTC‑GCGGGAACGC‑ATTCGCGCCACCCGTGATGATGCCGCCGTAAccg                                                                                                                                                                                                                                 <  2:347486/251‑1 (MQ=255)
                                 ccGGTGGCATGGCGAAAGCGATCGAAGCAGGTCTGCCAAAACGAATGATCGAAGAGGCCTCAGCGCGCGAACAGTCGCTGATCGACCAGGG‑CAAGCGTGTCA‑TCGTTGGTGT‑CAACAA‑GT‑ACAAA‑CTGGATCACGAAGA‑CGAAACCGATG‑TACTTGAGA‑TCGACAACGTGATGGTGCGTAACGAGCAAATTGCTTC‑GCTGGAACGC‑ATTCGCGCCACCCGTGATGATGCCGCCGTAACCGCCGCGTTGAAc                                                                                                                                                                                                                      >  1:409620/1‑251 (MQ=255)
                                   ggTGGCATGGCGAAAGCGATCGAAGCAGGTCTGCCAAAACGAATGATCGAAGAGGCCTCAGCGCGCGAACAGTCGCTGATCGACCAGGG‑CAAGCGTGTCA‑TCGTTGGTGT‑CAACAA‑GT‑ACAAA‑CTGGATCACGAAGA‑CGAAACCGATG‑TACTTGAGA‑TCGACAACGTGATGGTGCGTAACGAGCAAATTGCTTC‑GCTGGAACGC‑ATTCGCGCCACCCGTGATGATGCCGCCGTAAACGCCGCGTTGAACGc                                                                                                                                                                                                                    >  2:129322/1‑251 (MQ=255)
                                        cATGGCAAAAGCGATCGAAGCAGGTCTGCCAAAACGAATGTCCGAAGAGGCCTCAGCCCGCGAACAGTCGCTGATCGCCCAGGG‑CAAGCGTGTCA‑TCGTTGGTGT‑CAACAA‑GT‑ACAAC‑CTGGATCACGAAGA‑CGAAACCGTGG‑TACTTGAGA‑TCGACAACGTGATGGTGCGTAACGAGCAATTTGCTTC‑GCTGGAACGC‑ATTCGCGCCCCCCGTGATGATTCCGCCGTAACCGCCGCGTTGAACGCCCTGa                                                                                                                                                                                                               <  1:151207/251‑1 (MQ=255)
                                         aTGGCGAAAGCGATCGAAGCAGGTCTGCCAAAACGAATGATCGAAGAGGCCTCAGCGCGCGAACAGTCGCTGATCGACCAGGG‑CAAGCGTGTCA‑TCGTTGGTGT‑CAACAA‑GT‑ACAAA‑CTGGATCACGAAGA‑CGAAACCGATG‑TACTTGAGA‑TCGACAACGTGATGGTGCGTAACGAGCAAATTGCTTC‑GCTGGAACGC‑ATTCGCGCCACCCGTGATGATGCCGCCGTAACCGCCGCGTTGAACGCCCTGAc                                                                                                                                                                                                              >  1:381294/1‑251 (MQ=255)
                                             ggAAAGCGAGGGAAGCAGGCCTGCCAGACCGATGGATGGAAGCGGACTCGGCGTGCGAACAGTCGTTGATCGACCAGGG‑CCAGGGTGTCA‑TCTTTGGCGT‑GAACAA‑AT‑ACAAG‑AGGGATCAGGAAGA‑CGAAACCGATG‑TACTGGAAG‑TCGCCAACGTGTTGGTGCGGACCGATCAAATTGTGTG‑GCTGGGACGC‑AGTCGCTCCCCCCTTGATAATGCCGCCGTAACCGCCGCGTTGAACGCCCTGACTCAc                                                                                                                                                                                                          <  2:379605/250‑1 (MQ=255)
                                                aaGCGAGCAAAGCAGGTCTGCAAAAACGAAGGATCGAAGAGGCCTCAGCGCGCGAAAAGGCGTTGATCGACCAGGG‑AAAGGGTGTCA‑TCGTTGGTGT‑CAACAA‑GT‑ACAAA‑CGGGAGCACGAAGA‑CGAACCCGATG‑TACTTGAGA‑TCGACAACGTGATGGTGCGTAACGAGCAAATTGCTCC‑GCGGGAACGC‑TTTCGCGCCACCCGTGAGGATGCCGCCGTAACCGCCGCGTTGAACGCCCTGACTCAcgcc                                                                                                                                                                                                       <  2:21723/251‑1 (MQ=255)
                                                  gCGATCGAAGCCGGTCTGCCAAAACGCATGATCGCAGAAGCCTCCGCGAGCGAACTGTCGCTGATCGACCAGGG‑CAGGCGTGTCA‑TCGTTGGTGT‑CAACAA‑GT‑ACAAA‑CTGGATCACGAAGA‑CGAAACCGATG‑TCCTTGAGA‑TCGTCAACGAGATGGTGAGTAACGAGCCAATTGCTTC‑GCTGGAACGC‑ATTCGCGCCACCCGTGATGCTGCCGCCGTAACCGCCGCGTTAAACGCCCTGGCTCAcgccgc                                                                                                                                                                                                     >  2:63924/1‑251 (MQ=255)
                                                   ggATCGAAGCAGGTCTGCTAAAAAGAATGATCGAAGAGGCCTCTGCGCGGGAACAGTCTGTGATCG‑CCAGGGGCATGCGTTTCA‑TCGGTGGTGC‑CAACAA‑TT‑AAAAA‑CTGGATCAAGAAGA‑CGAACCCGATG‑TTCTGGAGA‑TCCAAACCGTGATGTTGTGTAACGAGCAAATTGTTTT‑GCTGGAACGC‑ATTCGCGCCCCCCGTTATGATGCCGCCGTAACCGCCGCGTTGAACGCCCTGACTCACGCCGca                                                                                                                                                                                                    <  1:483018/250‑1 (MQ=255)
                                                     aTCGAAGCAGGTCTGCCAACACGAATGATCGAAGAGGCCTCAGCGCGCGCACAGTCGCTGATCGACCAGGG‑CAAGCGTGTCA‑TCGTTGGTGT‑CAACAA‑GT‑ACAAA‑CTGGATCACGAAGA‑CGAAACCGATG‑TACTTGAGA‑TCGACAACGTGATGGTGCGTAACGAGCAAATTGCTTC‑GCTGGAACGC‑ATTCGCGCCACCAGTGATGATGCCGCCGTAACCGCCGCGTTGAACGCCCTGCCTCACGCCGcac                                                                                                                                                                                                   >  1:115558/1‑250 (MQ=255)
                                                        gAAGCAGGTCTGCCAAAACGAATGATCGAAGAGGCCTCAGCGCGCGAACAGTCGCTGATCGACCAGGG‑CAAGCGTGTCA‑TCGTTGGTGT‑CAACAA‑GT‑ACAAA‑CTGGATCACGAAGA‑CGAAACCGATG‑TACTTGAGA‑TCGACAACGTGATGGTGCGTAACGAGCAAATTGCTTC‑GCTGGAACGC‑ATTCGCGCCACCCGTGATGATGCCGCCGTAACCGCCGCGTTGAACGCCCTGACTCACGCCGCACAGc                                                                                                                                                                                                >  2:253935/1‑250 (MQ=255)
                                                        aaaacgggTCGGCCAAAGCGAATGAACGAAGTGGCCGAGGCGCGCGAACAGTCGGTGCCCG‑CCAGGGGCAAGGGGGCAA‑TCGTTGGGGA‑CAAAAA‑TT‑AAAAA‑CGGGATCCCAAAGA‑CGAACCCTATG‑TACTTGAGA‑TCGACAACGTGATGGTGCGTACCGAGAAAATTGCTTC‑GCTGAACCGC‑ATCCGCCCCCCCCGTGAGGATCCCGCCGTAACCGCCGCGTTGAACGCCCTGACTCACGCCGCACAGCa                                                                                                                                                                                               <  2:383337/245‑1 (MQ=255)
                                                         aaGCAGGTCTGCCAAAACGAATGATCGAAGAGGCCTCAGCGCGCGAACAGTCGCTGATCGACCAGGG‑CAAGCGTGTCA‑TCGTTGGTGT‑CAACAA‑GT‑ACAAA‑CTGGATCACGAAGA‑CGAAACCGATG‑TACTTGAGA‑TCGACAACGTGATGGTGCGTAACGAGCAAATTGCTTC‑GCTGGAACGC‑ATTCGCGCCACCCGTGATGATGCCGCCGTAACCGCCGCGTTGAACGCCCTGACTCACGCCGCACAGCAt                                                                                                                                                                                              >  2:435346/1‑251 (MQ=255)
                                                          aGCAGGTCTGCCAAAACGAATGATCGAAGAGGCCTCAGCGCGCGAACAGTCGCTGATCGACCAGGG‑CAAGCGTGTCA‑TCGTTGGTGT‑CAACAA‑GT‑ACAAA‑CTGGATCACGAAGA‑CGAAACCGATG‑TACTTGAGA‑TCGACAACGTGATGGTGCGTAACGAGCAAATTGCTTC‑GCTGGAACGC‑ATTCGCGCCACCCGTGATGATGCCGCCGTAACCGCCGCGTTGAACGCCCTGACTCACGCCGCACAGCAt                                                                                                                                                                                              >  1:237210/1‑250 (MQ=255)
                                                          aGCAGGTCTGCCAAAAAGAAGGATCGAAGAGGCCCCAGCGCGCGAACAGTCGCGGATCGACAAGGG‑CAAGCGTGTAA‑GCGTTGGTGT‑AAAAAA‑GT‑AAAAA‑CTGGATCACGAAGA‑CGAAACCGATG‑AACTGGAGA‑TCGACAACGGGAGGGGGCGTAACGAGAAAATTGCTTC‑GCTGGAACGC‑ATTCGCGCCACCCGTGATGATGCCGCCGTAACCGCCGCGTTGAACGCCCTGACTCACGCCGCACAGCATa                                                                                                                                                                                             <  1:79897/251‑1 (MQ=255)
                                                            gatgTCTGCCAAAACGAATGATCGAAGAGGCCTCAGCGCGCGAACAGTCGCTGATCGACCAGGG‑CAAGCGTGTCA‑TCGTTGGTGT‑CAACAA‑GT‑ACAAA‑CTGGATCACGAAGA‑CGAAACCGATG‑TACTTGAGA‑TCGACAACGTGATGGGGCGTAACGAGCAAATTGCTTC‑GCTGGAACGC‑ATTCGCGCAACCCGTGATGATGCCGCCGTAACCGCAGCGTTGAACGCCCTGACTCACGCCGCACAGCATAAc                                                                                                                                                                                           >  2:131490/4‑251 (MQ=255)
                                                            cAGGTCTGCCAAAACGAATGATCGAAGAGGCCTCAGCGCGCGAACAGTCGCTGATCGACCAGGG‑CAAGCGTGTCA‑TCGTTGGTGT‑CAACAA‑GT‑ACAAA‑CTGGATCACGAAGA‑CGAAACCGATG‑TACTTGAGA‑TCGACAACGTGATGGTGCGTAACGAGCAAATTGCTTC‑GCTGGAACGC‑ATTCGCGCCACCCGTGATGATGCCGCCGTAACCGCCGCGTTGAACGCCCTGACTCACGCCGCACAGCATAAc                                                                                                                                                                                           >  1:41588/1‑251 (MQ=255)
                                                             gggTCTGCCAAAACGATTGATCGAAGAGGCCGCAGCGCGCGAACAGTCGCTGATCGACCAGGG‑CAAGCGGGTCA‑TCTTTGGTGT‑CAACAA‑GT‑ACAAA‑CTGGATCACGAAGA‑CGAACCGGAGG‑TACTTGAGA‑TCGACAACGTGATGGTGCGTAACGAGAAAATTGCTTC‑GCTGGAACGC‑ATTCGCGCCCCCCGTGATGATGCCGCCGTAACCGCCGCGTTGAACGCCCTGACTCACGCCGCACAGCATAACg                                                                                                                                                                                          <  1:463916/250‑1 (MQ=255)
                                                             gggTCTGCCAAAACGAATGACCGAAGAGGCCTCAGGGCGCAAATAGTCGCTGATAGACCAGGG‑AAAGCGTGTCA‑TCGTTGGTTT‑CAACAA‑GT‑ACAGA‑CGGGATCACGAAGA‑CGAAACCGATT‑TACTTGAGA‑GCGACAAGGTGATGGGGCGTAATGAGCAAATTGCTCC‑GCGGGAACGC‑ATCCGCCCCCCCCGTGATGATGCCGCCGTAACCGCCGCGTTGAACGCCCTGACTCACGCCGCACAGCATAACg                                                                                                                                                                                          <  1:488676/250‑1 (MQ=255)
                                                             agggcTGCCAAAACGAATGATCGAATAGGCCTCAGCGCGCGAACAGTCGCTGATCGCCCAGGG‑CAAGCGTGTCA‑TCGTTGGTGT‑CAACAA‑GT‑ACAAC‑CTGGACCACGAAGA‑CGAACCCGATG‑TACTTGAGA‑TCGACAACGTGATGGTGCGTAACGAGCAAATTGCTTC‑GCTGGAACGC‑ATTCGCGCCCCCCGTGATGATGCCGCCGTAACCGCCGCGTTGAACGCCCTGACTCACGCCGCACAGCATAACg                                                                                                                                                                                          <  1:345957/247‑1 (MQ=255)
                                                                 cTGCCAAAACGAATGATCGAAGAGGCCTCAGCGCGCGAACAGTCGCTGATCGACCATGG‑CAAGCGTGTCA‑TCGTTGGTGT‑CAACAA‑GT‑ACAAA‑CTGGATCACGAAGA‑CGAAACCGATG‑TACTTGAGA‑TCGACAACGTGATGGTGCGTAACGAGCAAATTGCTTC‑GCTGGAACGC‑ATTCGCGCCACCCGTGATGATGCCGCCGTAACCGCCGCGTTGAACGCCCTGACTCACGCCGCACAGCATAACGaaa                                                                                                                                                                                       >  1:107217/1‑250 (MQ=255)
                                                                   gCCAAAACGAATGATCGAAGAGGCTCCAGCGCGGGAACAGTCGCTGATCGACCAGGG‑CAGGCGTGCAA‑CCGTTGGTGT‑CAAAAA‑GT‑ACAAA‑CGGGACCACGAAGA‑CGAAACCGATG‑TATTGGAGA‑TCGAAAACGTGAGGGTGCGTAACGAGAAAATTGCTTC‑GCTGGAACGC‑ATTCGCGCCACCCGTGATGATGCCGCCGTAACCGCCGCGTTGAACGCCCTGACTCACGCCGCACAGCATAACGAAAAc                                                                                                                                                                                     <  1:347103/250‑1 (MQ=255)
                                                                    ccAAAACGAATGATCGAAGAGGCCTCAGCGCGCGAACAGTCGCTGATCGACCAGGG‑CAAGCGTGTCA‑TCGTTGGTGT‑CAAAAA‑GT‑ACAAA‑CTGGATCACGAAGA‑CGAAACCGATG‑TACTTGAGA‑TCGACAACGTGATGTTGCGTAACGAGCAAATTGCTTC‑GCTGGAACGC‑ATTCGCGCCCCCCGTGATGATGCCGCCGTAACCGCCGCGTTGAACGCCCTGACTCACGCCGCACAGCATAACGAAAACct                                                                                                                                                                                   <  1:57759/251‑1 (MQ=255)
                                                                        aaCGAATGACGGAAGAGGCCTCAGCGCGCGAACAGTCGCTGATCGACCAGGG‑AAAGCGTGTCA‑CCGTTGGTGT‑CAACAA‑GT‑ACAAA‑CGGGATCACGAAGA‑CGAACCCGATG‑TCCTGGAGG‑TCGACAACGTGAGGGTGCGTAACGAGAAATTTGCTCC‑GCTGGAACGC‑ATTCGCCCCCCCCGGGATGAGGCCGCCGTAACCGCCGCGTTGAACGCCCTGACTCACGCCGCACAGCATAACGAAAACctgctg                                                                                                                                                                               <  2:211188/251‑1 (MQ=255)
                                                                            aaTGATCGAAGAGGCCCCAGCCAGCGAACAGTCGCTGACCGACCGGGC‑CAAGGGTGCCA‑TCTTGGGTGT‑CAACAA‑TT‑ACAAC‑CTGGATCACGAAGA‑CAAACCCGTTG‑TCCTGGAGA‑GCGACACCGTGATGGTGCGAAACGAGCAAATT‑CTTCCGCGGAAACGC‑AGTCGCCCCCCCCGGGATGATTCCGCCGTAACCGCCGCGTTGAACGCCCTGACTCACGCCGCACAGCATAACGAAAACCTgctggctg                                                                                                                                                                           <  2:324961/251‑1 (MQ=255)
                                                                              agagcGAAGAGGCCCCAGCGCGCGACCAGTCTCTGAGCGACCAGGG‑CAAGCGTTTCA‑TCTTTGGTGT‑CAACAA‑GT‑ACAAA‑CTGGACCACGAAGC‑CGAAACC‑ATGGTCCTTGGGA‑TCGCCAACGTGATGGTGCGTAACGAGCAAATGTCTTC‑GCTGGAACTC‑ATCCGCGCCCCCCTTGATGATGCCGCCGTAACCGCCGCGTTGAACGCCCTGACTCACGCCGCACAGCATAACGAAAACCTGCTGGCTGcc                                                                                                                                                                         <  1:197389/247‑1 (MQ=255)
                                                                               gaccgaggagGCCCCAGCGGGCAAACAGCCGCTGACCGACCAGGG‑CAAGCGTGTAA‑TCGT‑GGTGTACAACAA‑GT‑AAAAA‑CGGGACCAC‑AAGAACAAACCCGATT‑TACGTGAGA‑TCGACAACGTGGTGGGGGGTAACGAGAAAATGCCTTC‑GCTGGACCGA‑TTTCGGCCCCCCCGGGATGATCCCGCCGTAACCGCCGCGGTGAACGCCCTGACTCACGCCGCACAGCATAACGAAAACCTGCTGGCTGCCg                                                                                                                                                                        <  2:334979/244‑1 (MQ=255)
                                                                               gATCGAAGAGGCCTCAGCGCGCGAACAGTCGCTGATCGACCAGGG‑CAAGCGTGTCA‑TCGTTGGTGT‑CAACAA‑GT‑ACAAA‑CTGGATCACGAAGA‑CGAAACCGATG‑TACTTGAGA‑TCGACAACGTGATGGTGCGTAACGAGCAAATTGCTTC‑GCTGGAACGC‑ATTCGCGCCACCCGTGATGATGCCGCCGTAACCGCCGCGTTGAACGCCCTGACTCACTCCGCACAGCATAACGAAAACCTGCTGGCTGCCg                                                                                                                                                                        >  1:483168/1‑251 (MQ=255)
                                                                               gATCGAAGAGCCCCCAGCGCGCGACCAGTCGCTAATCGACCAGGG‑CAAGCGTGTTA‑TGTTTGGTGT‑CAACAA‑GT‑AAAAC‑CGGGATCCCGAAGC‑CGAAACCGATG‑TACTTGAGA‑TCGACAACGTGATGGTGCGTAACGATCAATTTGCTTC‑GCTGGACCGC‑ATTCGCGCCACCTGTTAGGATGCCGCCGTAACCGCCGCGTTGAACGCCCTGACTCACGCAGCACAGCATAACGGAAACCTGCTGGCTGcc                                                                                                                                                                         <  1:147053/250‑1 (MQ=255)
                                                                                  cGAAGAGGCCTCAGCGCGCGAACAGTCGCTGATCGACCAGGG‑CAAGCGTGTCA‑TCGTTGGTGT‑CAACAA‑GT‑ACAAA‑CTGGATCACGAAGA‑CGAAACCGATG‑TACTTGAGA‑TCGACAACGTGATGGTGCGTAACGAGCAAATTGCTTC‑GCTGGAACGC‑ATTCGCGCCACCCGTGATGATGCCGCCGTAACCGCCGCGTTGaa                                                                                                                                                                                                                       >  2:287305/1‑201 (MQ=255)
                                                                                  cGAAGAGGCCTCAGCGCGCGAACAGGCGCTGATCGACCAGGG‑CAGGCGTGTCA‑TCGTTGGTGT‑CAACAA‑GT‑ACAAA‑CTGGATCACGAAGA‑CGAAACCGATG‑TACTTGAGA‑TCGACAACGTGATGGTGCGGAACGAGCAAATTGCTTC‑GCGGGAACGC‑ATTCGCGCCACCCGTGATGATGCCGCCGTAACCGCCGCGTTGaa                                                                                                                                                                                                                       <  1:287305/201‑1 (MQ=255)
                                                                                    aaGAGGCCTAAGCGCGCAAACAGTCGCTGACAGACCACGG‑CCAGCGTGTCA‑TCGTTGGTGT‑CAGCAC‑GT‑ACAAA‑CTGGATCACGAAGA‑CGAAACCGATG‑TACTTGAGA‑TCGACAACGTGATGGTGCGTAACGAGCAAATTGCTTC‑GCTGGAACGC‑ATTCGCGCAACCCGTGATGATGCCGCCGTAACCGCCGCGTTGAACGCCCTGCCTCACGCCGCACAGCCTAACGAACACCTGCCGGCTGCCGCTGtt                                                                                                                                                                   >  2:336636/1‑251 (MQ=255)
                                                                                      gagGCCTCAGCGCGCGAACAGTCGCTGCTCGACCAGGG‑CAAGCGTGTCA‑TCGTTGGTGT‑CAACAA‑GT‑ACAAA‑CTGGATCACGAAGA‑CGAAACCGATG‑TACTTGAGA‑TCGACAACGTGATGGTGCGTAACGAGCAAATTGCTTC‑GCTGGAACGC‑ATTCGCGCCACCCGGGATGATGCCGCCGTAACCGCCGCGTTGAACGACCTGCCTCACGCCGCACAGCATAACGGAAACCTGCTGGCTGCCGCTGttt                                                                                                                                                                  >  1:34791/1‑249 (MQ=255)
                                                                                      gagGCCTCAGCGCGCGAACAGTCGCTGATCGATCAGGG‑CAAGCGTGTCA‑TCGTTGGTGT‑CAACAA‑GT‑ACAAA‑CTGGATCACGAAGA‑CGAAACCGATG‑TACTTGAGA‑TCGACAACGTGATGGTGCGTAACGAGCAAATTGCTTC‑GCTGGAACGC‑ATTCGCGCCACCAGTGATGATGCCGCCGTAACCGCCGCGTTGAACGCCCTGACTCACGCCGCACAGCATAACGACAACCTGCTGGCTGCCGCTGTTa                                                                                                                                                                  >  2:171819/1‑250 (MQ=255)
                                                                                         gCCTCAGCGCGCGAACAGTCGCTGACCGACCAGGG‑CAGGGGTTTCA‑CCGTGGGTGT‑‑AAAAA‑GTTAAAAA‑CTGGATACCGAAGC‑CAAACCCAATT‑GCCTTGGGA‑TCGACAGGGGGAGGGTGCGAAACGAGCAAATGTCTTC‑GCTGGAACGC‑ATCCGCGCCCCCCGGGAGGATGCCGCCGTAACCGCCGCGTTGAACGCCCTGTCTCACGCCGCACAGCATAACGAAAACCTGCTGGCTGCCGCTGTTAATGc                                                                                                                                                              <  2:290244/251‑1 (MQ=255)
                                                                                           cTCAGCGCGCGAACAGTCGCTGATCGACCAGGG‑CAAGCGTGTCA‑TCGTTGGTGT‑CAACAA‑GT‑ACAAA‑CTGGATCACGAAGA‑CGAAACCGATG‑TACTTGAGA‑TCGACAACGTGATGGTGCGTAACGAGCAAATTGCTTC‑GCTGGAACGC‑ATTCGCGCCACCCGTGATGATGCCGCCGTAACCGCCGCGTTGAACGCCCTGACTCACGCCGCACAGCATAACGAAAACCTGCTGGCTGCCGCTGTTAATGCCg                                                                                                                                                            >  2:428490/1‑251 (MQ=255)
                                                                                            tCAGCGCGCGAACAGTCGCTGATCGACCAGGG‑CAAGCGTGTCA‑TCGTTGGTGT‑CAACAA‑GT‑ACAAA‑CTGGATCACGAAGA‑CGAAACCGATG‑TACTTGAGA‑TCGACAACGTGATGGTGCGTAACGAGCAAATTGCTTC‑GCTGGAACGC‑ATTCGCGCCACCCGTGATGATGCCGCCGTAACCGCCGCGTTGAACGCCCTGACTCACGCCGCACAGCATAACGAAAACCTGCTGGCTGCCGCTGTTAATGCCGc                                                                                                                                                           >  1:288122/1‑251 (MQ=255)
                                                                                              agctcgcgaacgggcgtttaTCGACCAGGA‑CAAGCGTGGCA‑TCGTTGGTGT‑CAAAAA‑TA‑AAAAA‑CTGGATCACGAAGC‑CGAAACCGATG‑TACTTGAGA‑TCGACAACGTGATGGTGCGTAACGAGCAATTTTCTTC‑GCTGGACCGC‑TTGCGCGCCACCCGTGAGGATGCCGCCGTAACCGCCGCGTTGAACGCCCTGACTCACGCCGCACAGCATAACGAAAACCTGCTGGCTGCCGCTGTTAATGCCGCTc                                                                                                                                                         <  1:168967/232‑1 (MQ=255)
                                                                                                 gcgcgAACAGCCTCGGATCGCCCAGGG‑CAAGCGT‑TCAATCGTGGGTGT‑CAACAA‑GT‑ACAAA‑CTGGATCACGAAGA‑CGAAACCGATG‑AACTTGAGA‑TCGACAACGTGATGGTGCGTACCGAGCAAATTGCTTC‑GGTGGAACGC‑ATTCGCGCCCCCCGTGATGATGCCGCCGTAACCGCCGCGTTGAACGCCCTGACTCACGCCGCACAGCATAACGAAAACCTGCTGGCTGCCGCTGTTAATGCCGCTcgcg                                                                                                                                                      <  1:476850/251‑1 (MQ=255)
                                                                                                  cgcgAACAGTCGCTGATCGACCAGGG‑CAAGCGTGTCA‑TCGTTGGTGT‑CAACAA‑GT‑ACAAA‑CTGGATCACGAAGA‑CGAAACCGATG‑TACTTGAGA‑TCGACAACGTGATGGTGCGTAACGAGCAAATTGCTTC‑GCTGGAACGC‑ATTCGCGCCACCCGTGATGATGCCGCCGTAACCGCCGCGTTGAACGCCCTGACTCACGCCGCACAGCATAACGAAAACCTGCTGGCTGCCGCTGTTAATGCCGCTCGCGt                                                                                                                                                     >  1:224593/1‑251 (MQ=255)
                                                                                                            cGCTGACCGACCGGGG‑CAAGCGTGTCA‑TCGTAGGGGC‑CAACAA‑G‑‑ACAAAACTGCATCACAAAGA‑CGACCCCGATT‑CATTGGAGA‑TCGACACCGGGAGGGTGAGAAACGAGAAATTTGCTTC‑CCGGGAACGC‑TTTCGCGCCCCCCGTGATGAGGCCGCCGTAACCGCCGCGTTGAACGCCCTGACTCACGCCGCACAGCATAACGAAAACCTGCTGGCTGCCGCTGTTAATGCCGCTCGCGTTCGCGCCAcc                                                                                                                                           <  2:193918/251‑1 (MQ=255)
                                                                                                             gCTGATCGACCAGGG‑CAAGCGTGTCA‑TCGTTGGTGT‑CAACAA‑GT‑ACAAA‑CTGGATCACGAAGA‑CGAAACCGATG‑TACTTGAGA‑TCGACAACGTGATGGTGCGTAACGAGCAAATTGCTTC‑GCTGGAACGC‑ATTCGCGCCACCCGTGATGATGCCGCCGTAACCGCCGCGTTGAACGCCCTGACTCACGCCGCACAGCATAACGAAAACCTGCTGGCTGCCGCTGTTAATGCCGCTCGCGTTCGCGCCAccc                                                                                                                                          >  2:430788/1‑251 (MQ=255)
                                                                                                                    gACCAGGG‑CAAGCGTGTCA‑TCGTTGGTGT‑CAACAA‑GT‑ACAAA‑CTGGATCACGAAGA‑CGAAACCGATG‑TACTTGAGA‑TCGACAACGTGATGGTGCGTAACGAGCAAATTGCTTC‑GCTGGAACGC‑ATTCGCGCCCCCCGTGATGATGCCGCCGTAACCGCCGCGTTGAACGCCCTGACTCACGCCGCACAGCATAACGAAAACCTGCTGGCTGCCGCTGTTAATGCCGCt                                                                                                                                                          <  1:145402/228‑1 (MQ=255)
                                                                                                                      ccAGGG‑CAAGCGTGTCA‑TCGTTGGTGT‑CAACAA‑GT‑ACAAA‑CTGGATCACGAAGA‑CGAAACCGAGG‑TACTTGAGA‑TCGACAACGTGATGGTGCGTAACGAGCAAATTTCTTC‑GCTGGACCGC‑ATTCGCGCCCCCCTTGATGATGCCGCCGTAACCGCCGCGTTGAACGCCCTGACTCACGCCGCACAGCATAACGAAAACCTGCTGGCTGCCGCTGTTAATGCCGCTCGCGTTCGCGCCACCCTGGGTGaaa                                                                                                                                 <  1:252381/251‑1 (MQ=255)
                                                                                                                      ccAGGG‑CAAGCGGGTCA‑TCTTGGGTGA‑CAACAA‑GT‑ACAAA‑CGGGATCGCAAAGA‑CAAAACCGTTG‑TCATTGAGA‑TCCACAACGGTAGGGGGCGTAACGAGCAAATTGCTTC‑GCTGGAACGC‑ATCCGCCCCCCCCGTGATGATGCCGCCGTAACCGCCGCGTTGAACGCCCTGACTCACGCCGCACAGCATAACGAAAACCTGCTGGCTGCCGCTGTTAATGCCGCTCGCGTTCGCGCCACCCTGGGTGaaa                                                                                                                                 <  2:237210/251‑1 (MQ=255)
                                                                                                                           g‑CAAGCGTGTCA‑TCGTTGGTGT‑CAACAA‑GT‑ACAAA‑CTGGATCACGAAGA‑CGAAACCGATG‑TACTTGAGA‑TCGACAACGTGATGGTGCGTAACGAGCAAATTGCTTC‑GCTGGAACGC‑ATTCGCGCCACCCGTGATGATGCCGCCGTAACCGCCGCGTTGAACGCCCTGACTCACGCCGCACAGCATAACGAAAACCTGCTGGCTGCCGCTGTTAATGCCGCTCGCGTTCGCGCCACCCTGGGTGAAATTTcc                                                                                                                            >  1:269407/1‑251 (MQ=255)
                                                                                                                             cAAGGGTGTCA‑TCGTTGGTGT‑‑AACAAAGA‑ACAAA‑CGGGATCACGAAGC‑CGAACCGGATG‑TACTTGAGA‑GCGACACCGGGAGGGGGCGTAACAAGAAAATTGCTTC‑‑CGGGGACGCCATCCGCGCCACCGGTGATGATGCCGCCGTAACCGCCGCGTTGAACGCCCTGACTCACGCCGCACAGCATAACGAAAACCTGCTGGCTGCCGCTGTTAATGCCGCTCGCGTTCGCGCCACCCTGGGTGAAATTTCCg                                                                                                                           <  2:259733/251‑1 (MQ=255)
                                                                                                                                  gtgtCA‑TCGTTGGTGT‑CAACAA‑GT‑ACAAA‑CTGGATCACGAAGA‑CGAAACCGATT‑TACTTGAGA‑TCGACAACGTGATGGTGCGTAACGAGCAAATTGCTTC‑GCTGGAACGC‑ATGCGCGCCCCCCGGGATGATGCCGCCGTAACCGCCGCGTTGAACGCCCTGACTCACGCCGCACAGCATAACGAAAACCTGCTGGCTGCCGCTGTTAATGCCGCTCGCGTTCGCGCCACCCTGGGTGAAATTTCCGATgcg                                                                                                                      <  1:469873/251‑1 (MQ=255)
                                                                                                                                  gtgtCA‑TCGTTGGTGT‑CAACAA‑GT‑ACAAA‑CTGGATCACGAAGA‑CGAAACCGATG‑TACTTGAGA‑TCGACAACGTGATGGTGCGTAACGAGCAAATTGCTTC‑GCTGGAACGC‑ATTCGCGCCACCCGTGATGATGCCGCCGTAACCGCCGCGTTGAACGCCCTGACTCACGCCGCACAGCATAACGAAAACCTGCTGGCTGCCGCTGTTAATGCCGCTCGCGTTCGCGCCACCCTGGGTGAAATTTCCGATgcg                                                                                                                      >  1:421138/1‑251 (MQ=255)
                                                                                                                                   ggtCA‑TGGTTGGTGC‑CAACAG‑GT‑ACAAA‑CTGGTCCCCGAAGA‑CGAACCCGATT‑TACTGGAGA‑CCAACAACGTGATGGTGGGTAACGAGCAATTTGCTTC‑CCTGGA‑CGCCTTTCGCGCCACCCTTGAGGATGCCGCCGTAACCGCCGCGTTGAACGCCCTGACTCACGCCGCACAGCATAACGAAAACCTGCTGGCTGCCGCTGTTAATGCCGCTCGCGTTCGCGCCACCCTGGGTGAAATTTCCGATgcgc                                                                                                                     <  1:18916/250‑1 (MQ=255)
                                                                                                                                     tCA‑TCGTTGGTGT‑CAACAA‑GT‑ACAAA‑CTGGATCACGAAGA‑CGAAACCGATG‑TACTTGAGA‑TCGACAACGTGATGGTGCGTAACGAGCAAATTGCTTC‑GCTGGAACGC‑ATTCGCGCCACCCGTGATGATGCCGCCGTAACCGCCGCGTTGAACGCCCTGACTCACGCCGCACAGCCTAACGAAAACCTGCTGGCTGCCGCTGTTAATGCCGCTCGCGTTCGCGCCACCCTGGGTGAAATTTCCGATGCGCTg                                                                                                                   >  2:478215/1‑251 (MQ=255)
                                                                                                                                          cGTTGGTGT‑CAACAA‑GT‑ACAAA‑CTCGCTCACGAAGA‑CGAAACCGTTG‑TCCTTGAGA‑TCGAAAACGTCATGGTGCGTCACGCGCAAATTGCTTC‑GCTGGAACGC‑ATTAGCGCCACCCGTGATGATGCCGCCGTAACCGCCGCGTTGAACGCCCTGACTCACGCCGCACAGCATAACGCAAAACTGCTGGCTGCCGCTGTTAATGCCGCTCGCGTTCGCGCCACCCTGGGCGAACTTTCCGATGCGCTGGa                                                                                                                 >  2:54336/1‑249 (MQ=255)
                                                                                                                                                tgt‑CAACAA‑GT‑ACAAA‑CTGGATCACGAAGA‑CGAAACCGATG‑TACTTGAGC‑TCGACAACGTGATGGTGCGTAACGAGCAAATTGCTTC‑GCTGGAACGC‑ATTCGCGCCACCCGTGATGATGCCGCCGTAACCGCCGCGTTGAACGCCCTGACTCACGCCGCACAGCATAACGAAAACCTGCTGGCTGCCGCTGTTAATGCCGCTCGCGTTCGCGCCACCCTGGGTGAAATTTCCGATGCGCTGGAAGTCGCtt                                                                                                         >  1:464135/1‑251 (MQ=255)
                                                                                                                                                      caaaaatgaCAAA‑CTGGTTCACGAAGC‑AAAAACCGATG‑TACTTCAGA‑TCGACACCGTGATGGTGCGAAACGAGCAAATTGCTTC‑GCGGGAACTC‑TTTCGCGCCACCCGTGATGATGCCGCCGTAACCGCCGCGTTGAACGCCCTGACTCACGCCGCACAGCATAACGAAAACCTGCTGGCTGCCGCTGTTAATGCCGCTCTCGTTCGCGCCACCCTGGGTGAAATTTCCGATGCGCTGGAAGTCGCTTTc                                                                                                       <  1:85230/242‑1 (MQ=255)
                                                                                                                                                            gaaaaaa‑CTGGATCACGAAGA‑GAAAACCGAGT‑TACTT‑AGAATCGACACCGGGAGGGGGCGTAACGAGAAAAGTGCTTC‑GCGGAAACGC‑ATTCGCCCCCCCCTGGATGATGCCGCCGTAACCGCCGCGTTGAACGCCCTGACTCACGCCGCACAGCATAACGAAAACCTGCTGGCTGCCGCTGTTAAGGCCGCGCGCGTTCGCGCCACCCTGGGTGAAATTTCCGATGCGCTGGAAGTCGCTTTCGACCGtt                                                                                                <  2:49217/247‑1 (MQ=255)
                                                                                                                                                               cAAA‑CTGGATCACGAAGA‑CGAAACCGATG‑TACTTGAGA‑TCGACAACGTGATGGTGCGTAACGAGCAAATTGCTTC‑GCTGGAACGC‑ATTCGCGCCACCCGTGATGATGCCGCCGTAACCGCCGCGTTGAACGCCCTGACTCACGCCGCACAGCATAACGAAAACCTGCTGGCTGCCGCTGTTAATGCCGCTCGCGTTCGCGCCACCCTGGGTGAAATTTCCGATGCGCTGGAAGTCGCTTTCGACCGTTATc                                                                                             >  1:333941/1‑251 (MQ=255)
                                                                                                                                                                            cGAAGA‑AGAAACCGATG‑TTCTTGAGA‑TCGACACTGTGAGGGTGCGTAACGAGCAAATTGTTTC‑GCTGGAACGC‑ATTCGCCCCACCCGTGATTTTGCCGCCGTAACCGCCGCGTTGAACGCCCTGACTCACGCCGCACAGCATAACGAAAACCTGCTGGCTGCCGCTGTTAATGCCGCTCGCGTTCGCGCCACCCTGGGTGAAATTTCCGATGCGCTGGAAGTCGCTTTCGACCGTTATCTGGTGCCAAg                                                                                   <  1:146191/249‑1 (MQ=255)
                                                                                                                                                                               gaagacaacaacgatataattGAGA‑TCGACAACGTGATGGAGCGTAACGAGCAAATTGCTTC‑GCTGGAACGC‑ATTAGCGCCACCAGAGATAATGACGCCGTAACAGCCGCGTTGAACGCCCTGCCTAACGCCGCACAGCATAACGAAAACCTGATGGCTGCCGCTGTTAATGCCGCTCGCGTTCGCGCCACCCTGGGTAAAATTTCCGATGCGCAGGAAGTCGCTTTCGAACGTTATCTGGTGCCAAGccc                                                                                >  2:429236/20‑250 (MQ=255)
                                                                                                                                                                               aGA‑CGAAACCGATG‑TACTTGAGA‑TCGACAACGTGATGGTGCGTAACGAGCAAATTGCTTC‑GCTGGAACGC‑ATTCGCGCCACCCGTGATGATGCCGCCGTAACCGCCGCGTTGAACGCCCTGACTCACGCCGCACAGCATAACGAAAACCTGCTGGCTGCCGCTGTTAATGCCGCTCGCGTTCGCGCCACCCTGGGTGAAATTTCCGATGCGCTGGAAGTCGCTTTCGACCGTTATCTGGTGCCAAGCCAgt                                                                              >  1:375457/1‑251 (MQ=255)
                                                                                                                                                                                gA‑CGAAACCGATG‑TACTTGAGA‑TCGACAACGTGATGGTGCGTAACGAGCAAATTGCTTC‑GCTGGAACGC‑ATTCGCGCCACCCGTGATGATGCCGCCGTAACCGCCGCGTTGAACGCCCTGACTCACGCCGCACAGCATAACGAAAACCTGCTGGCTGCCGCTGTTAATGCCGCTCGCGTTCGCGCCACCCTGGGTGAAATTTCCGATGCGCTGGAAGTCGCTTTCGAcc                                                                                                   >  1:244646/1‑229 (MQ=255)
                                                                                                                                                                                       aCCGATG‑AACTGGAAA‑TCGACAACGGTATGGTGCGTAACGAGCAATTGGCTTC‑CCTGAAACGC‑ATGCGCGCCACCCGTGATGAGCCCGCCGTAACCGCCGCGTTGAACGCCCTGACTCACGCCGCACAGCATAACGAAAACCTGCTGGCTGCCGCTGTTAATGCCGCTCGCGTTCGCGCCACCCTGGGTGAAATTTCCGATGCGCTGGAAGTCGCTTTCGACCGTTATCTGGTGCCAAGCCAGTGTGTTAc                                                                       <  1:68973/251‑1 (MQ=255)
                                                                                                                                                                                        ccGATG‑TACTTGAGA‑TCGACAACGTGATGGTGCGTAACGAGCAAATTGCTTC‑GATGGAACGC‑ATTCGCGCCACCCGTGATGATGCCGCCGTAACCGACGCGTTGAACGCCCTGACTCACGCCGCACAGCATAACGAAAACCTGCTGGCTGCCGCTGTTAATGCCGCTCGCGTTCGCGCCACCCTGGGTGAAATTTCAGATGCGCTGGAAGTCGCTTTCGACCGTTATCTGGTGCCAAGCCAGTGTGTTAcc                                                                      >  2:481077/1‑251 (MQ=255)
                                                                                                                                                                                         cGATG‑TACTTGACA‑TCGACAACGTGATGGTGCTTAACGAGCAAATTGCTTC‑GCTGGAACGC‑ATTCGCGCCCCCCGTGATGTTGCCGCCGTAACCGCCGCGTTGAACGCCCTGACTCACGCCGCACAGCATAACGAAAACCTGCTGGCTGCCGCTGTTAATGCCGCTCGCGTTCGCGCCACCCTGGGTGAAAGTTCCGATGCGCTGGAAGTCGCTTTCGACCGTTATCTGGTGCCAAGCCAGTGTGTTACCg                                                                     <  1:62422/251‑1 (MQ=255)
                                                                                                                                                                                           gttgttcTTGATA‑TCGAAAACGTGAGGGTGCGAAACGGGCAAATTGCTTC‑GCGGGACCCC‑ATTCGCGCCCCCCTTGAGGATGCCGCCGTAACCGCCGCGTTGAACGCCCTGACTCACGCCGCATAGCATAACGAAAACCTGCTGGCTGCCGCTGTTAATGCCGCTCGCGTTCGCGCCACCCTGGGTGAAATTTCCGATGCGCTGGAAGTCGCTTTCGACCGTTATCTGGTGCCAAGCCAGTGTGTTACCgg                                                                    <  1:387040/245‑1 (MQ=255)
                                                                                                                                                                                           aTG‑TACTTGAGA‑TCGACAACGTGATGGTGCGTAACGAGCAAATTGCTTC‑GCTGGAACGC‑ATTCGCGCCACCCGTGATGATGCCGCCGTAACCGCCGCGTTGAACGCCCTGACTCACGCCGCACAGCATAACGAAAACCTGCTGGCTGCCGCTGTTAATGCCGCTCGCGTTCGCGCCACCCTGGGTGAAATTTCCGATGCGCTGGAAGTCGCTTTCGACCGTTATCTGGTGCCACGCCAGTGTGTTACCGGc                                                                   >  2:405076/1‑251 (MQ=255)
                                                                                                                                                                                                aCTTGAGA‑TCGACAACGTGATGGTGCGTAACGAGCAAATTGCTTC‑GCTGGAACGC‑ATTCGCGCCACCCGTGATGATGCCGCCGTAACCGCCGCGTTGAACGCCCTGACTCACGCCGCACAGCATAACGAAAACCTGCTGGCTGCCGCTGTTAATGCCGCTCGCGTTCGCGCCACCCTGGGTGAAATTTCCGATGCGCTGGAAGTCGCTTTCGACCGTTATCTGGTGCCAAGCCAGTGTGTTACCGGCGTg                                                                >  1:275053/1‑250 (MQ=255)
                                                                                                                                                                                                  tggaga‑TCGACAAGGTGATGGGGCGAAACGAGAAAATGGCTTC‑GCGGGAACGC‑ATTCGCGCCCCCCGTGATGAGGCCGCCGTAACCGCCGCGTTGAACGCCCTGACTCACGCCGCACAGCATAACGAAAACCTGCTGGCTGCCGCTGTTAATGCCGCTCGCGTTCGCGCCACCCTGGGTGAAATTTCCGATGCGCTGGAAGTCGCTTTCGACCGTTATCTGGTGCCAAGCCAGTGTGTTACCGGCGTGAt                                                              <  2:381294/248‑1 (MQ=255)
                                                                                                                                                                                                     aga‑TCGACAACGTGATGGTGCGTAACGAGCAAATTGCTTC‑GCTGGAACGC‑ATTCGCGCCACCCGTGATGATGCCGCCGTAACCGCCGCGTTGAACGCCCTGACTCACGCCGCACAGCATAACGAAAACCTGCTGGCTGCCGCTGTTAAt                                                                                                                                                                >  2:117742/1‑149 (MQ=255)
                                                                                                                                                                                                     aga‑TCGACAACGTGATGGTGCGTAACGAGCAAATTGCTTC‑GCTGGAACGC‑ATTCGCGCCACCCGTGATGATGCCGCCGTAACCGCCGCGTTGAACGCCCTGACTCACGCCGCACAGCATAACGAAAACCTGCTGGCTGCCGCTGTTAAt                                                                                                                                                                <  1:117742/149‑1 (MQ=255)
                                                                                                                                                                                                            acaccgggtgggTGCGTAACGGGCAAATTGCTTC‑GCGGGACCCC‑ATCCGCGCCACCGGTGATGATGCCGCCGTAACCGCCGCGTTGAACGCCCTGACTCACGCCGCACAGCATAACGAAAACCTGCTGGCTGCCGCTGTTAATGCCGCTCGCGTTCGCGCCACCCTGGGTGAAATTTCCGATGCGCTGGAAGTCGCTTTCGACCGTTATCTGGTGCCAAGCCAGTGTGTTACCGGCGTGATTGCGCAAAGc                                                    <  2:319331/241‑1 (MQ=255)
                                                                                                                                                                                                             cAACGTGATGGTGCGTAACGAGCAAATTGCTTC‑GCTGGAACGC‑ATTCGCGCCACCCGTGATGATGCCGCCGTAACCGCCGCGTTGAACGCCCTGACTCACGCCGCACAGCATAACGAAAACCTGCTGGCTGCCGCTGTTAATGCCGCTCGCGTTCGCGCCACCCTGGGTGAAATTTCCGATGCGCTGGAAGTCGCTTTCGACCGTTATCTGGTGCCAAGCCAGTGTGTTACCGGCGTGATTGCGCAAAGCt                                                   >  1:247292/1‑251 (MQ=255)
                                                                                                                                                                                                             cAACGTGATGGTGCGTAACGAGCAAATTGCTTC‑GCTGGAACGC‑ATTCGCGCCACCCGTGATGATGCCGCCGTAACCGCCGCGTTGAACGCCCTGACTCACGCCGCACAGCATAACGAAAACCTGCTGGCTGCCGCTGTTAATGCCGCTCGCGTTCGCGCCACCCTGGGTGAAATTTCCGATGCGCTGGAAGTCGCTTTCGACCGTTATCTGGTGCCAAGCCAGTGTGTTACCGGCGTGATTGCGCAAAGCt                                                   >  1:119027/1‑251 (MQ=255)
                                                                                                                                                                                                              aaCGTGATGATGCGTAACGAGAAAATTGCTTC‑GATGGAACGC‑ATTCGCGCCACCCGTGATGACGCCGCCGTAACCGCCGCACTGAACGCCCTGACTCACGCCGCACAGCATAACGAAACCCTGCTGGCTGCCGCTGTTAATGCCGATCGCGTTCGCGCCACCCTGGGTGAAATTTCCGATACGCTGGACGTCGCTTTCGACCGTTATCTGGTGCCAAGCCAGCGTGTTACCGGCGTCATTGCGCAAAGCt                                                   >  2:235649/1‑250 (MQ=255)
                                                                                                                                                                                                                 gTGATGGTGCGTAACGAGCAAATTGCTTC‑GCTGGAACGC‑ATTCGCGCCACCCGTGATGATGCCGCCGTAACCGCCGCGTTGAACGCCCTGACTCACGCCGCACAGCATAACGAAAACCTGCTGGCTGCCGCTGTTAATGCCGCTCGCGTTCGCGCCACCCTGGGTGAAATTTCCGATGCGCTGGAAGTCGCTTTCGACCGTTATCTGGTGCCAAGCCAGTGTGTTACCGGCGTGATTGCGCAAAGCTatcc                                               >  2:165103/1‑250 (MQ=255)
                                                                                                                                                                                                                    aTGGTGCGTAACGAGCAAATTGCTTC‑GCTGGAACGC‑ATCCGCGCCACCCGGGATGATGCCGCCGTAACCGCCGCGTTGAACGCCCTGACTCACGCCGCACAGCATAACGAAAACCTGCTGGCTGCCGCTGTTAATGCCGCTCGCGTTCGCGCCACCCTGGGTGAAATTTCCGATGCGCTGGAAGTCGCTTTCGACCGTTATCTGGTGCCAAGCCAGTGTGTTACCGGCGTGATTGCGCAAAGCTAtcatca                                            <  1:409299/251‑1 (MQ=255)
                                                                                                                                                                                                                     tGGTGCGTAACGAGCACATTGCTTC‑GCTGGAAAGC‑ATTCGCGCCACCCGTGATGATGCCGCCGTAACCCCCGCATTGACCGACCTGCCAAACGCCGCACAGCATAACGCAAACCTGATGGCTGCCGCTGTTAATGCCGCTCGCGTTCGCGCCCCCCTGGGAGACATTTCCGATGCGCTGGAAGTAGCTTTCGACCGTTATCTGATGCCCCGCCAGCGTGTTACCGGCGCGATTTCGCAACGCGatcattcg                                           >  2:285915/1‑248 (MQ=255)
                                                                                                                                                                                                                       gTGCGTAACGAGCAAATTGCTTC‑GCTGGAACGC‑ATTCGCGCCACCCGTGATGATGCCGCCGTAACCGCCGCGTTGAACGCCCTGACTCACGCCGCACAGCATAACGAAAACCTGCTGGCTGCCGCTGTTAATGCCGCTCGCGTTCGCGCCACCCTGGGTGAAATTTCCGATGCGCTGGAAGTCGCTTTCGACCGTTATCTGGTGCCAAGCCAGTGTGTTACCGGCGTGATTTCGCAAAGCTATCATCAGTc                                         >  1:388938/1‑251 (MQ=255)
                                                                                                                                                                                                                             aaCGAGCAAATTGCTTC‑GCTGGAACGC‑ATTCGCGCCACCCGTGATGATGCCGCCGTAACCGCCGCGTTGAACGCCCTGACTCACGCCGCACAGCATAACGAAAACCTGCTGGCTGCCGCTGTTAATGCCGCTCGCGTTCGCGCCACCCTGGGTGAAATTTCCGATGCGCTGGAAGTCGCTTTCGACCGTTATCTGGTGCCCAGCCAGTGTGTTACCGGAGTGATTGCGCAAAGCTAACATCAGTATgaga                                    >  2:187263/1‑250 (MQ=255)
                                                                                                                                                                                                                                gggcaagttccTTC‑GCTGGACCGC‑GTTCCCGCCCCCCGGGTTGAGGCCGCCGTAACCGCCGCGTTGAACGCCCTGACGCACGCCGCACAGCATAACGAAAACCTGCTGGCTGCCGCTGTTAATGCCGCTCGCGTTCGCGCCACCCTGGGTGAAATTTCCGATGCGCTGGAAGTCGCGTTCGACCGTTATCTGGTGCCAAGCCAGTGTGTTACCGGCGTGATTGCGCAAAGCTATCCTCAGTCTGAGAAATc                                <  1:463577/241‑1 (MQ=255)
                                                                                                                                                                                                                                 aGCAAATTGCTTC‑GCTGGAACGC‑ATTCGCGCCACCCGTGATGCTGCCGCCGTAACCGCCGCGTTGAACGCCCTGACTCACGCCGCACAGCATAACGAAAACCTGCTGGCTGCCGCTGTTAATGCCGCTCGCGTTCGCGCCACCCTGGGTGAAATTTCCGATGCGCTGGAAGTAGCTTTCGACCGTTATCTGGTGCCAAGCCAGTGTGTTACCGGCGTGATTGCGCCAAGCTATCCTCAGTCTGAGAAATCg                               >  2:44585/1‑251 (MQ=255)
                                                                                                                                                                                                                                            tC‑GCTGAAACTC‑AGTCGCGCCACCCGTGATGATGCCGCCGTAACCGCCGCGTTGAACGCCCTGACTCACGCCGCACAGCATAACGAAAACCTGCTGGCTGCCGCTGTTAATGCCGCTCGCGTTCGCGCCACCCTGGGTGAAATTTCCGATGCGCTGGAAGTCGCTTTCGACCGTTATCTGGTGCCAAGCCAGTGTGTTACCGGCGTGATTGCGCAAAGCTATCATCAGTCTGAGAAATCGGCCTCCGAg                      <  1:478356/249‑1 (MQ=255)
                                                                                                                                                                                                                                               gCTGGAACGC‑ATTCGCGCCACCCGTGATGATGCCGCCGTAACCGCCGCGTTGAACGCCCTGACTCACGCCGCACAGCATAACGAAAACCTGCTGGCTGCCGCTGTTAATGCCGCTCGCGTTCGCGCCACCCTGGGTGAAATTTCCGATGCGCTGGAAGTCGCTTTCGACCGTTATCTGGTGCCAAGCCAGTGTGTTACCGGCGTGATTGCGCAAAGCTCTCATCAGTCTGAGAAATCGGCCTCCGAGTTCg                  >  1:225473/1‑251 (MQ=255)
                                                                                                                                                                                                                                                  ggAACGC‑ATTCGCGCCACCCGTGATGATGCCGCCGTAACCGCCGCGTTGAACGCCCTGACTCACGCCGCACAGCATAACGAAAACCTGCTGGCTGCCGCTGTTAATGCCGCTCGCGTTCGCGCCACCCTGGGTGAAATTTCCGATGCGCTGGAAGTCGCTTTCGACCGTTATCTGGTGCCAAGCCAGTGTGTTACCg                                                                     >  1:213595/1‑197 (MQ=255)
                                                                                                                                                                                                                                                  ggAACGC‑ATGCGCGCCACCCGGGATGATGCCGCCGTAACCGCCGCGTTGAACGCCCTGACTCACGCCGCACAGCATAACGAAAACCTGCTGGCTGCCGCTGTTAATGCCGCTCGCGTTCGCGCCACCCTGGGTGAAATTTCCGATGCGCTGGAAGTCGCTTTCGACCGTTATCTGGTGCCAAGCCAGTGTGTTACCGGCGTGATTGCGCAAAGCTATCATCAGTCTGAGAAATCGGCCTCCGAGTTCGa                 <  1:325520/249‑1 (MQ=255)
                                                                                                                                                                                                                                                   gAACGC‑ATTCGCGCCACCCGTGATGATGCCGCCGTAACCGCCGCGTTGAACGCCCTGACTCACGCCGCACAGCATAACGAAAACCTGCTGGCTGCCGCTGTTAATGCCGCTCGCGTTCGCGCCACCCTGGGTGAAATTTCCGATGCGCTGGAAGTCGCTTTCGACCGTTATCTGGTGCCAAGCCAGTGTGTTACCGGCGTGATTGCGCAAAGCTATCATCAGTCTGAGAAATCGGCCTCCGAGTTCGATGc              >  1:185802/1‑251 (MQ=255)
                                                                                                                                                                                                                                                    aaCGC‑ATTCGCGCCACCCGTGATGATGCCGCCGTAACCGCCGCGTTGAACGCCCTGACTCACGCCGCACAGCATAACGAAAACCTGCTGGCTGCCGCTGTTAATGCCGCTCGCGTTCGCGCCACCCTGGGTGAAATTTCCGATGCGCTGGAAGTCGCTTTCGACCGTTATCTGGTGCCAAGCCAGTGTGTTACCGGCGTGATTGCGCAAAGCTATCATCAGTCTGAGAAATCGGCCTCCGAGTTCGATGcc             >  1:53413/1‑251 (MQ=255)
                                                                                                                                                                                                                                                     aacccatccgcgCCACCCGGGATGATGCCGCCGTAACCGCCGCGTTGAACGCCCTGACTCACGCCGCACAGCATAACGAAAACCTGCTGGCTGCCGCTGTTAATGCCGCTCGCGTTCGCGCCACCCTGGGTGAAATTTCCGATGCGCTGGAAGTCGCTTTCGACCGTTATCTGGTGCCAAGCCAGTGTGTTACCGGCGTGATTGCGCAAAGCTATCATCAGTCTGAGAAATCGGCCTCCGAGTTCGATGcc             <  1:253935/243‑1 (MQ=255)
                                                                                                                                                                                                                                                        gaaTTCGCGCAACCCGTGATGATGCCGCCGTAACCGCCGCGTTGAACGCCCTGCCTCACGCCGCACCGCATAACGAAAACCTGCTGGCTGCCGCTGTTAATGCCGCTCGCGTTCGCGCCACCCTGGGTGACATTTCCGATGCGCTGGAAGTCGCTTTCGACCGTTATCTGGTGCAAAGCCAGTGTGTTACCGGCGTGCTTGCGCACAGCTATCTTCATTCTGAGAAATCGGCCTTCTAGTGCGATGACAtt          >  2:470635/3‑251 (MQ=255)
                                                                                                                                                                                                                                                                 ccACCCGTGATGATGCCGCCGTAACCGCCGCGTTGAACGCCCTGACTCACGCCGCACAGCATAACGAAAACCTGCTGGCTGCCGCTGTTAATGCCGCTCGCGTTCGCGCCACCCTGGGTGAAATTTCCGATGCGCTGGAAGTCGCTTTCGACCGTTATCTGGTGCCAAGCCAGTGTGTTACCGGCGTGATTGCGCAAAGCTATCATCAGTCTGAGAAATCGGCCTCCGAGTTCGATGCCATTGTTGCGCa  >  2:111226/1‑250 (MQ=255)
                                                                                                                                                                                                                                                                   aCCCGTGATGATGCCGCCGTAACCGCCGCGTTGAACGCCCTGACTCACGCCGCACAGCATAACGAAAACCTGCTGGCTGCCGCTGTTAATGCCGCTCGCGTTCGCGCCACCCTGGGTGAAATTTCCGATGCGCTGGAAGTCGCTTTCGACCGTTATCTGGTGCCAAGCCAGTGTGTTACCGGCGTGATTGCGCAAAGCTAtcatca                                            >  1:57019/1‑206 (MQ=255)
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AAGCCAGAGCTATTATCCAACAGATCGACGAAGCCGGTGGCATGGCGAAAGCGATCGAAGCAGGTCTGCCAAAACGAATGATCGAAGAGGCCTCAGCGCGCGAACAGTCGCTGATCGACCAGGG‑CAAGCGTGTCA‑TCGTTGGTGT‑CAACAA‑GT‑ACAAA‑CTGGATCACGAAGA‑CGAAACCGATG‑TACTTGAGA‑TCGACAACGTGATGGTGCGTAACGAGCAAATTGCTTC‑GCTGGAACGC‑ATTCGCGCCACCCGTGATGATGCCGCCGTAACCGCCGCGTTGAACGCCCTGACTCACGCCGCACAGCATAACGAAAACCTGCTGGCTGCCGCTGTTAATGCCGCTCGCGTTCGCGCCACCCTGGGTGAAATTTCCGATGCGCTGGAAGTCGCTTTCGACCGTTATCTGGTGCCAAGCCAGTGTGTTACCGGCGTGATTGCGCAAAGCTATCATCAGTCTGAGAAATCGGCCTCCGAGTTCGATGCCATTGTTGCGCA  >  NC_000913/3062072‑3062567

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑