Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,014,222	0	C	A	28.8%	66.6 / 11.4	52	Q332K (CAA→AAA)	fliF	flagellar basal‑body MS‑ring and collar protein
Reads supporting (aligned to +/- strand):  ref base C (25/12);  new base A (1/14);  total (26/26)
Fisher's exact test for biased strand distribution p-value = 1.16e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

GTCGCCTATTGTTGGTAACGGTAATATTCACGCCCAGGTTACGGCGCAGCTGGACTTCGCCAGTAAAGAACAAACGGAAGAACAGTATCGCCCTAACGGTGATGAATCTCATGCGGCGCTTCGTTCACGCCAGCTTAATGAGAGCGAGCAAAGCGGTTCCGG‑TTATCCGG‑GCGGCGTACCGGGGG‑CGTTGTCGAATCAACCGGCACCTG‑CGAATAACGCGCCAATCAGCACGCCTCCG‑GCAAATCAAAATAA‑CCGCCAGCAGCAGGCGAGCACCACCAGCAATAGTGGGCCGCGTAGCACACAGCGGAATGAAACCAGTAACTACGAAGTCGATCGCACCATTCGTCATACCAAAATGAACGTGGGCGATGTGCAACGTCTGTCAGTCGCGGTCGTGGTGAATTACAAAACCTTGCCAGATGGCAAACCGTTGCCTCTCAGCAACGAACAGATGAAGCAAATTGAAGATCTGACCCGCGAGGCGATGG  >  NC_000913/2013978‑2014465                                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                     gTCGCCTATTGTTGGTAACGGTAATATTCACGCCCAGGTTACGGCGCAGCTGGACTTCGCCAGTAAAGAACAAACGGAAGAACAGTATCGCCCTAACGGTGATGAATCTCATGCGGCGCTTCGTTCACGCCAGCTTAATGAGAGCGAGCAAAGCGGTTCCGG‑TTATCCGG‑GCGGCGTACCGGGGG‑CGTTGTCGAATCAACCGGCACCTG‑CGAATAACGCGCCAATCAGCACGCCTCCG‑GCAAATCaaaa                                                                                                                                                                                                                                                  <  1:329515/249‑1 (MQ=255) gTCGCCTATTGTTGGTAACGGTAATATTCACGCCCAGGTTACGGCGCAGCTGGACTTCGCCAGTAAAGAACAAACGGAAGAACAGTATCGCCCTAACGGTGATGAATCTCATGCGGCGCTTCGTTCACGCCAGCTTAATGAGAGCGAGCAAAGCGGTACCGG‑TTATCCGG‑GCGGCGTACCGGGGG‑CGCTGTCGAATCAACCGACACCCG‑CGCATAACGCGCCAATCAGCACGCCGACG‑GCAACTCAAAAt                                                                                                                                                                                                                                                 >  2:102298/1‑250 (MQ=255) gTCGCCTATTGTTGGTAACGGTAATATGCACGCCCAGGGTACGGCGCAGCTGGACTTCGCCAGTAAAGAACAAACGGAAGAACAGTATCGCCCTAACGGTGATGAATCTCATGCGGCGCTTCGTTCACGCCAGCTTAATGAGAGCGAGCAAAGCGGTTCCGG‑TTAGCCGG‑GCGGCGTACCGGGGG‑CGTTGTCGAATCAACCGGCACCTG‑CGAATAACGCGCCAATCAGCACGCCTCCG‑GCAAATCAAAAt                                                                                                                                                                                                                                                 <  2:178804/250‑1 (MQ=255)         ttgttgGTAACGGTAATATTCACGCCCAGGTTACGGCGCAGCTGGACTTCGCCAGTAAAGAACAAACGGAAGAACAGTATCGCCCTAACGGTGATGAATCTCATGCGGCGCTTCGTTCACGCCAGCTTAATGAGAGCGAGCAAAGCGGTTCCGG‑TTATCCGG‑GCGGCGTACCGGGGG‑CGTTGTCGAATCAACCGGCACCTG‑CGAATAACGCGCCAATCAGCACGCCTCCG‑GCAAATCAAAATAA‑CCGcc                                                                                                                                                                                                                                         >  1:502937/1‑249 (MQ=255)                                                        ttCGCCAGTAAAGAACAAACGGAAGAACAGTATCGCCCTAACGGTGATGAATCTCATGCGGCGCTTCGTTCACGCCAGCTTACTGAGAGCGAGCAAAGCGGTTCCGG‑TTATCCGG‑GCGGCGTACCGGGGG‑CGTTGTCGAATCAACCGGCACCTG‑CGAATAACGCGCCAATCAGCACGCCTCCG‑GCAAATCAAAATAA‑CCGCCAGCAGCAGGCGCGCACCACCCGCAATAGAGGGCCGCGTAAcacacaacg                                                                                                                                                                                        >  1:345631/1‑248 (MQ=255)                                                           gCCAGTAAAGAACAAACGGAAGAACAGTATCGCCCTAACGGTGATGAATCTCATGCGGCGCTTCGTTCACGCCAGCTTAATGAGAGCGAGCAAAGCGGTTCCGG‑TTATCCGG‑GCGGCGTACCGGGGG‑CGTTGTCGAATCAACCGGCACCTG‑CGAATAACGCGCCAATCAGCACGCCTCCG‑GCAAATCAAAATAA‑CCGCCAGAAGCAGGCGAGCACCACCAGCAATAGTGGGCCGCGTAGCACACAGCGGa                                                                                                                                                                                      >  2:138257/1‑250 (MQ=255)                                                                    gAACAAACGGAAGAACAGTATCGCCCTAACGGTGATGAATCTCATGCGGCGCTTCGTTCACGCCAGCTTAATGAGAGCGAGCAAAGCGGTTCCGG‑TTATACGG‑GCGGCGTACCGGGGG‑CGTTGTCGAATCAACCGGCACCTG‑CGAATAACGCGCCAATCAGCACGCCTCCG‑GCAAATCAAAATAA‑CCGCCAGCAGCAGGCGAGCACCACCAGCAATAGTGGGCCGCGTAGCACACAGCGGAATGAAAcc                                                                                                                                                                              >  2:355583/1‑249 (MQ=255)                                                                    aaaCAAACGGAAGAACATGAGCCCCTTAACGGTGTGGTATCTCATGGGGCGTTGCGTTCACGCCAGTTAAAGGAGAGGGAGAAAAGCGGTTCGGG‑TT‑CCCGGTGCGGCGT‑CCGGGGGTGGTTGTCAAAGAAACCGGCCCCGG‑GAAATAACGCTTGAAGCAGCACGCCTCCG‑GAAAAGCAAAATAA‑CCGCCAGCAGCAGGCGAGCACCACCAGCAATAGTGGGCCGCGTAGCACACAGCGGAATGAAACCAg                                                                                                                                                                            <  1:467598/250‑1 (MQ=255)                                                                      aCAAACGGAAGAACAGTATCGCCCTAACGGTGATGAATCTCATGCGGCGCTTCGTTCACGCCAGCTTAATGAGAGCGAGCAAAGCGGTTCCGG‑TTATCCGG‑GCGGCGTACCGGGGG‑CGTTGTCGAATCAACCGGCACCTG‑CGAATAACGCGCCAATCAGCACGCCTCCG‑GCAAATCCAAATAA‑CCGCCAGCAGCAGGCGAGCACCACCAGCAATAGTGGGCCGCGTAGCACACAGCGGAATGAAACCAGt                                                                                                                                                                           >  2:96571/1‑250 (MQ=255)                                                                      aCAAACGGAAGAACAGTATCGCCCTAACGGTGATGAATCTCATGCGGCGCTTCGTTCACGCCAGCTTAATGAGAGCGAGCAAAGCGGTTCCGG‑TTATCCGG‑GCGGCGTACCGGGGG‑CGTTGTCGAATCAACCGGCACCTG‑CGAATAACGCGCCAATCAGCACGCCTCCG‑GCAAATCAAAATAA‑CCGCCAGCAGCAGGCGAGCACCACCAGCAATAGAGGGCCGCGTAGCCCACAGAGGAATGAAACCAGt                                                                                                                                                                           >  2:96580/1‑250 (MQ=255)                                                                        aaaCGGAAGAACAGTATCGCCCTAACGGTGATGAATCTCATGCGGCGCTTCGTTCACGCCAGCTTAATGAGAGCGAGCAAAGCGGTTCCGG‑TTATCCGG‑GCGGCGTACCGGGGG‑CGTTGTCGAATCAACCGGCACCTG‑CGAATAACGCGCCAATCAGCACGCCTCCG‑GCAAATCAAAATAA‑CCGCCAGCAGCAGGCGAGCACCACCAGCAATAGTGGGCCGCGTAGCACACAGCGGAATGAAACCAGTAAc                                                                                                                                                                        >  2:493973/1‑251 (MQ=255)                                                                                aaacagaaccccccTAACGGTGATAAACCTCATGCGGCGCTTCGTCCACGCCAGCTTAATGAGAGCGAGCAAAGCGTTTCCGG‑TTACCCGG‑GCGGCGTACCGGGGG‑CGTTGTCGAATCAACCGGCCCCTG‑CAAATAACGCGCCAATCAGCCCGCCTCCG‑GCAAATCAAAATAA‑CCGCCAGCAGCAGGCGAGCACCACCAGCAATAGTGGGCCGCGTAGCACACAGCGGAATGAAACCAGTAACTACGAAGt                                                                                                                                                                <  1:413564/240‑1 (MQ=255)                                                                                 aaCAGTATCGCCTTAACGGGGATGAACCGCATGCGGCGCTTCGTTCACGCCAGCTTAAGGAGAGCGAGCAAAGCGGTCCGGG‑TTATCCGG‑GCGGCGTCCCGGGGG‑CGTTGTCGAATCAACCGGCACCTG‑CGAATAACGGCCCAATCAGCACGCCTCCG‑GAAAATAAAAAAAA‑CCGCCAGCAGCAGGCGAGCACCACCAGCAATAGTGGGCCGCGTAGCACACAGCGGAATGAAACCAGTAACTACGAAGTc                                                                                                                                                               <  2:298697/251‑1 (MQ=255)                                                                                     gTATCGCCTTAACGGTGATGAATCCCATGCGCCGCTTCGTCCACGCCAGCTTAAGGAGAGCGAGAAAAGCGGTTCCGG‑TTATCGGG‑GCGGCGTACCGGGGG‑GGTTGTCGAACCACCCGCCACCTG‑CGAAGAACGCGCCAATCAGCACGCCCCCG‑GCAAACCAAAAAAA‑CCGCCAGCAGCAGGCGAGCACCACCAGCAATAGTGGGCCGCGTAGCACACAGCGGAATGAAACCAGTAACTACGAAGTCGATc                                                                                                                                                           <  1:9053/251‑1 (MQ=255)                                                                                       gtCGCCCGAGCGGTGAGGAATCTCAGGGGGCGCGTCGTTCACGCAAGTTAAATGAGTGGGAGCAAGGGGGTTCGGG‑TTACCCGG‑GCGCGGTACCGGGGG‑CGTTGTCGAACCACCCGGCACCTG‑CGAAAAACGCGCCAATCAGCCCGCCTCCG‑GAAAATAAAAAGAA‑CCGCCAGCAGCAGGCGAGCACCACCAGCAATAGTGGGCCGCGTAGCACACAGCGGAATGAAACCAGTAACTACGAAGTCGATCg                                                                                                                                                          <  2:477563/249‑1 (MQ=255)                                                                                               accGGTGATGAATCTCATGCGGCTCTTCTTTCCCGCCAGTTTAAGGAGAGCGTGCAAAGCGGTTCCGG‑TTACCCGG‑GGGGCGTCCCGGGGG‑CGTTGCCGAATCACCCGCCCCCTG‑CGAATACCGCGCCAACCACCCCCCCCCCG‑GCAAATCAAAATAA‑CCGCCATCAGCAGGCGAGCACCACCAGCAATAGTGGGCCGCGTAGCACACAGCGGAATGAAACCAGTAACTACGAAGTCGATCGCACCAtt                                                                                                                                                   <  1:132743/247‑1 (MQ=255)                                                                                                 cGGTGATGAATCTCATGCGGCGCTTCGTTCACGCCAGCTTAATGAGAGCGAGCAAAGCGGTTCCGG‑TTATCCGG‑GCGGCGTACCGGGGG‑CGTTGTCGAATCAACCGGCACCTG‑CGAATAACGCGCCAATCAGCACGCCTCCG‑GCAAATCAAAATAA‑CCGCCAGCAGCAGGCGAGCACCACCAGCAATAGTGGGCCGCGTAGCACACAGCGGAATGAAACCAGTAACTACGAAGTCGATCGCACCATTCGTc                                                                                                                                               >  1:52033/1‑251 (MQ=255)                                                                                                      atgaATCTCATGCGGCGCTGCGTTCCCGCCAGCTTAAGGAGAGCGAGCAAAGGGGTTCCGG‑TTATCCGG‑GCGGCGTACCGGGGG‑CTTTGGCGAACCAACCGGCCCCTG‑GAAATAACGCGCCAATCAGCGCGCCTCCG‑GCAAATAAAAATAA‑CCGCCAGCAGCAGGCGAGCACCACCAGCAATAGTGGGCCGCGTAGCACACAGCGGAATGAAACCAGTAACTACGAAGTCGATCGCACCATTCGTCATAcc                                                                                                                                          <  1:209003/251‑1 (MQ=255)                                                                                                         aaTCTCATGCGGCGCTTCGTTCACGCCAGCTTAATGAGAGCGAGCAAAGCGGTTCCGG‑TTATCCGG‑GCGGCGTACCGGGGG‑CGTTGTCGAATCAACCGGCACCTG‑CGAATAACGCGCCAATCAGCACGCCTCCG‑GCAAATCAAAATAA‑CCGCCAGCAGCAGGCGAGCACCACCAGCAATAGTGGGCCGCGTAGCACACAGCGGAATGAAACCAGTAACTACGAAGTCGATCGCACCATTCGTCATACCaa                                                                                                                                        >  2:379064/1‑250 (MQ=255)                                                                                                               aggcggcgCTTCGTTCACGCCAGTTGAATGAGAGGGACCAAAGCGGTTCCGG‑TTACCCGG‑GCGGCGTACCGGGGG‑CGTTGTCGAACCACCCGGCACCTG‑CGAATAACGCGCCAATCAGCACGCCCCCG‑GCAAATAAAAATAA‑CCGCCAGCAGCAGGCGAGCACCACCAGCAATAGTGGGCCGCGTAGCACACAGCGGAATGAAACCAGTAACTACGAAGTCGATCGCACCATTCGTCATACCAAAATGAAc                                                                                                                                 <  1:10120/249‑1 (MQ=255)                                                                                                                 ggggcgCTTCGTTCACGCCAGTTTAAGGAGAGCGATAAAAGCGGTCCCGG‑TTACCGGG‑GCGGCGTCCGGGGGG‑CGTTGCCGAATCAACCGGCCCCTG‑AAAATAACGCGCAAAGCACCCCGCCCCGG‑GAAAAACAAAATAA‑CCGCCAGCAGCAGGCGAGCACCACCAGCAATAGTGGGCCGCGTAGCACACAGCGGAATGAAACCAGTAACTACGAAGTCGATCGCACCATTCGTCATACCAAAATGAACGt                                                                                                                               <  2:430863/249‑1 (MQ=255)                                                                                                                   ggcgtttcgttccgcccggattAATGAGCGCAAGCAAAGCGTTTCGGG‑TTATCGGG‑GCGGCGTCCCGGGGT‑CTTTGTCAAACCACCCGGC‑CCTGGCTAAAAACGCGACAATCAGCACGCCTCCG‑GCAAATAAAAATAA‑CCGCCAGCAGCAGGCGAGCACCACCAGCAATAGTGGGCCGCGTAGCACACAGCGGAATGAAACCAGTAACTACGAAGTCGATCGCACCATTCGTCATACCAAAATGAACGTgg                                                                                                                             <  1:386245/231‑1 (MQ=255)                                                                                                                   ggcgCTTCGTTCACGCCAGTTTAATGAGAGCGAGAAAAGCGGTTCCGG‑TTACCGGG‑GCGCCGTACCGGGGG‑CGTTGCCAAACCACCCGGCACCTG‑CAAATAACGCGCCAATCAGCCCGCCTCCG‑GCAAATAAAAATAA‑CCGCCAGCAGCAGGCGAGCACCACCAGCAATAGTGGGCCGCGTAGCACACAGCGGAATGAAACCAGTAACTACGAAGTCGATCGCACCATTCGTCATACCAAAATGAACGTgg                                                                                                                             <  1:139622/251‑1 (MQ=255)                                                                                                                     ggcTTCGTTCACGCCAGTTTAATGAGAGCGAGCAAAGCGGTTCCGG‑TTATCCGG‑GCGACGTACCGGGGG‑CGATGTCGCATGAACAGGCAACTG‑CGAATAACGCGCCAATCAGCACGCCACCG‑GAAAATCAAAATAA‑CAGCCAGCAGCAGGCGAGCACCACCAGACGTAGTGGGCCACGAAGCACACGGCGGAATGAACCCATTAACTACGAAGTCGAACGCACCATACGTCCTACCAAAATGAAcagggtg                                                                                                                           >  2:168973/2‑245 (MQ=255)                                                                                                                            ggcccGCCAGCTTAAGGGGAGGGAGCAAAGCGGTTCCGG‑TTACCCGG‑GCGGCGGACCGGGGG‑CGGTGTCGATGCAACCGGCACCTG‑CAAATACCGCGCCAATCAGCACGCCACCG‑GCAAA‑CAAAAAAAGCCGCCAGCAGCAGGCGAGCACCACCAGCAATAGTGGGCCGCGTAGCACACAGCGGAATGAAACCAGTAACTACGAAGTCGATCGCACCATTCGTCATACCAAAATGAACGTGGGCGATGTGc                                                                                                                    <  2:4900/247‑1 (MQ=255)                                                                                                                                    ggCTTAAGGAGGGGGAGCAAAGCGGTTCCGG‑TTATCCGG‑GCGGGGTACCGGGGG‑CGTTGCCAAAAAAACCGCCCCCTG‑AGAATACCGCGCCAATCGGCCCCCCCCCG‑GCAAAACAAAATAA‑CCGCCAGCAGCAGGCGAGCACCACCAGCAATAGTGGGCCGCGTAGCACACAGCGGAATGAAACCAGTAACTACGAAGTCGATCGCACCATTCGTCATACCAAAATGAACGTGGGCGATGTGCAACGTCTg                                                                                                            <  2:143863/250‑1 (MQ=255)                                                                                                                                       ttAATGAAAGCGAGCAAAGCGGTTCCGG‑TTCTCCAG‑GCTGCGTACAGGGGG‑CGTTGTCGAATCAACCGGCACCTG‑CGACTCACGCGCCAATCAGCACGCCTCCG‑GCACATCAAAATAA‑CCGCCAGCAGCAGGCGAGCACCACCAGCCATAGTGGGCCGCGTAGCACACAGCGGAATGAAACCCATAACTACGAAGGCGCACGAACCATTCGTCATACCAAACTGAACAGGCGCGCTGTGCAACGTCTGTc                                                                                                          >  2:217763/1‑250 (MQ=255)                                                                                                                                             agagCGAGCAAAGCGGTTCCGG‑TTATCCGG‑GCGGCGTACCGGGGG‑CGTTGTCGAATCAACCGGCACCTG‑CGAATAACGCGCCACTCAGCACGCCTCCG‑GCAAATCAAAATAA‑CCGCCAGCAGCAGGCGAGCACCACCAGCAATAGTGGGCCGCGTAGCACACAGCGGAATGAAACCAGTAACTACGAAGTCGATCGCACCATTCGTCATACCAAAATGAACGTGGGCGATGTGCACCGTCTGTCAGTcgcg                                                                                                   >  2:325795/1‑251 (MQ=255)                                                                                                                                                gcgagcAAAGCG‑TTCCGGGTTAGCGGG‑GCGGCGTACCGGGGG‑CGTTGACCAACCAACCGCCACCTG‑CAAATAACGCGCCAATCAGCCCGCCCCCG‑GCAAAACAAAATAA‑CCGCCAGCAGCAGGCGAGCACCACCAGCAATAGTGGGCCGCGTAGCACACAGCGGAATGAAACCAGTAACTACGAAGTCGATCGCACCATTCGTCATACCAAAATGAACGTGGGCGATGTGCAACGTCTGTCAGTCGCGGTc                                                                                                <  2:175935/251‑1 (MQ=255)                                                                                                                                                 ggagcAAAGCGCTCCCTG‑TTACCCGG‑GGGGCGTACCGGGGG‑CGTTGGAGAATCAACCGCCCCCTG‑CGAATAACGCGCCATACAGCCCGCCTCCG‑GCAAATAAAAATAA‑CCGCCAGCAGCAGGCGAGCACCACCAGCAATAGTGGGCCGCGTAGCACACAGCGGAATGAAACCAGTAACTACGAAGTCGATCGCACCATTCGTCATACCAAAATGAACGTGGGCGATGTGCAACGTCTGTCAGTCGCGGTCg                                                                                               <  2:77632/250‑1 (MQ=255)                                                                                                                                                  gagcAAAGCGGTTCCGG‑TTATCCGG‑GCGGCGTACCGGGGG‑CGTTGTCGAATCAACCGGCACCTG‑CGAATAACGCGCCAATCAGCACGCCTCCG‑GCAAATCAAAATAA‑CCGCCAGCAGCAGGCGAGCACCACCAGCAATAGTGGGCCGCGTAGCACACAGCGGAATGAAACCAGTAACTACGAAGTCGATCGCACCATTCGTCATACCAAAATGAACGTGGGCGATGTGCAACGTCTGTCAGTCGCGGTCgg                                                                                              >  1:57848/1‑250 (MQ=255)                                                                                                                                                      aaaGCGGTTCCGG‑TTATCCGG‑GCGGCGTACCGGGGG‑CGTTGTCGAATCAACCGGCACCTG‑CGAATAACGCGCCAATCCGCACGCCTCCG‑GCAAATCAAAATAA‑CCGCCAGCAGCAGGCGAGCACCACCCGCAATAGTGGGCCGCGTAGCACACAGCGGAATGAAACCAGTAACTACGAAGTCGATCGCACCATTCGTCATACCAAAATGAAAGAGGGCGATGTGCAACGGCTGACAGTCGCGGTCgtggtg                                                                                          >  2:414592/1‑251 (MQ=255)                                                                                                                                                      aaaGCGGTTCCGG‑TTATCCGG‑GCGGCGTACCGGGGG‑CGTTGTCGAATCAACCGGCACCTG‑CGAATAACGCGCCAATCAGCACGCCTCCG‑GCAAATCAAAATAA‑CCGCCAGCAGCAGGCGAGCACCACCAGCAATAGTGGGCCGCGTAGCACACAGCGGAATGAAACCAGTAACTACGAAGTCGATCGCACCATTCGTCATACCAAAATGAACGTGGGCGATGTGCAACGTCTGTCAGTCGCGGTCgtggtg                                                                                          >  2:297241/1‑251 (MQ=255)                                                                                                                                                            gTTCCGG‑TTATCCGG‑GCGGCGTACCGGGGG‑CGTTGTCGAATCAACCGGCACCTG‑CGAATAACGCGCCAATCAGCACGCCTCCG‑GCAAATCAAAATAA‑CCGCCAGCAGCAGGCGAGCACCACCAGCAATAGTGGGCCGCGTAGCACACAGCGGAATGAAACCAGTAACTACGAAGTCGATCGCACCATTCGTCATACCAAAATGAACGTGGGCGATGTGCAACGTCTGTCAGTCGCGGTCGTGGTGAATTa                                                                                     >  1:164608/1‑250 (MQ=255)                                                                                                                                                                    aatccggg‑gCGGCGTACCGGGGG‑CGTTGGCGAATCACCCGGCACCTG‑CAAATAACGCCCCAATCAGCACGCCTCCG‑GAAAATAAAAATAA‑CCGCCAGCAGCAGGCGAGCACCACCAGCAATAGTGGGCCGCGTAGCACACAGCGGAATGAAACCAGTAACTACGAAGTCGATCGCACCATTCGTCATACCAAAATGAACGTGGGCGATGTGCAACGTCTGTCAGTCGCGGTCGTGGTGAATTACAAAACCt                                                                             <  1:345572/245‑1 (MQ=255)                                                                                                                                                                         cGG‑GCGGCGTACCGGGGG‑CGTTGTCGAATCAACCGGCACCTG‑CGAATAACGCGCCAATCAGCACGCCTCCG‑GCAAATCAAAATAA‑CCGCCAGCAGCAGGCGAGCACCACCAGCAATAGTGGGCCGCGTAGCACACAGCGGAATGAAACCAGTAACTACGAAGTCGATCGCACCATTCGTCATACCAAAATGAACGTGGGCGATGTGCAACGTCTGTCAGTCGCGGTCGTGGTGAATTACAAAACCt                                                                             >  2:345572/1‑246 (MQ=255)                                                                                                                                                                           g‑gcggcGTACCGGGGG‑CGTTGTCGAATCAACCGGCACCTG‑CGAATAACGCGCCAATCAGCACGCCTCCG‑GCAAATCAAAATAA‑CCGCCAGCAGCAGGCGAGCACCACCAGCAATAGTGGGCCGCGTAGCACACAGCGGAATGAAACCAGTAACTACGAAGTCGATCGCACCATTCGTCATACCAAAATGAACGTGGGCGATGTGCAACGTCTGTCAGTCGCGGTCGTGGTGAATTACAAAACCTTGcc                                                                         >  1:134511/1‑248 (MQ=255)                                                                                                                                                                                 cgTACCGGGGG‑CGTTGGCGACACACCCGGCACCTG‑CGAAAAACGCGCCAACCAGCCCTCC‑CCGAGAAAACAAAAATAA‑CCGCCAGCAGCAGGCGAGCACCACCAGCAATAGTGGGCCGCGTAGCACACAGCGGAATGAAACCAGTAACTACGAAGTCGATCGCACCATTCGTCAGACCAAAATGAACGTGGGCGATGTTCAACGTCTGTCAGTCGCGGTCGTGGTGAATTACAAAACCTTGCCAGATGGCa                                                                 <  1:27819/251‑1 (MQ=255)                                                                                                                                                                                      cGGGGG‑CGTTGTCGAATCAACCGGCACCTG‑CGAATAACGCGCCAATCAGCACGCCTCCG‑GCAAATCAAAATAA‑CCGCCAGCAGCAGGCGAGCACCACCAGCAATAGTGGGCCGCGTAGCACACAGCGGAATGAAACCAGTAACTACGAAGTCGATCGCACCATTCGTCATACCAAAATGAACGTGGGCGATGTGCAACGTCTGTCAGTCGCGGTCGTGGTGAATTACAAAACCTTGCCAGATGGCAAACCg                                                            >  1:347497/1‑251 (MQ=255)                                                                                                                                                                                          gggccgtgccGAATCAACCCACACATG‑CGAAGCACGCGCCAATCAGCACCCCTCCG‑GCAAAAAAAAAAAA‑CCGCCAGCAGCAGGCGAGCACCACCAGCAATAGTGGGCCGCGTAGCACACAGCGGAATGAAACCAGTAACTACGAAGTCGATCGCACCATTCGTCATACCAAAATGAACGTGTGCGATGTGCAACGTCTGTCAGTCGCGGTCGTGGTGAATTACAAAACCTTGACAGATGGCAAACCGTTg                                                         <  2:487867/242‑1 (MQ=255)                                                                                                                                                                                              gTTGTCGAACCAACCGGCCCCTC‑CGAATAACGCGCCAATCAGCCCCCCTCCG‑GCAAACCAAAAAAA‑CCGCCAGCAGCAGGCGAGCACCACCAGCAATAGTGGGCCGCGTAGCACACAGCGGAATGAAACCAGTAACTACGAAGTCGATCGCACCATTCGTCATACCAAAATGAACGTGGGCGATGTGCAACGTCTGTCAGTCGCGGTCGTGGTGAATTACAAAACCTTGCCAGATGGCAAACCGTTGCctc                                                     <  1:28215/251‑1 (MQ=255)                                                                                                                                                                                                    gAATCAACCGGCACCTG‑CGAATAACGCGCCAATCAGCACGCCTCCG‑GCAAATCAAAATAA‑CCGCCAGCAGCAGGCGAGCACCACCAGCAATAGTGGGCCGCGTAGCACACAGCGGAATGAAACCAGTAACTACGAAGTCGATCGCACCATTCGTCATACCAAAATGAACGTGGGCGATGTGCAACGTCTGTCAGTCGCGGTCGTGGTGAATTACAAAACCTTGCCAGATGGCAAACCGTTGCCTCTCAGc                                                >  1:398068/1‑250 (MQ=255)                                                                                                                                                                                                    caaacaacccggcccctgaaaaTACCGCCCCAACCAGCACGCCTCCG‑GCAAATAAAAATAC‑CCGCCAGCAGCAGGCGAGCACCACCAGCAATAGGGGGCCGCGTAGCACACAGCGGAATGAAACCAGTAACTACGAAGTCGATCGCGCCATTCGTCATACCAAAATGAACGTGGGCGATGGGCAACGTCTGTCAGTCGCGGGCGTGGTGAATTACAAAACCTTGCCAGATGGCAAACCGTTGCCACTCAg                                                 <  2:502937/230‑1 (MQ=255)                                                                                                                                                                                                            cGGCACCTG‑CGAATAACGCGCCAATCAGCACGCCTCCG‑GCAAATCAAAATAC‑CCGCCAGCAGCAGGCCAGCACCACCAGCAATAGTGGGCCGCGTAGCACACAGCGGAATCAAACCAGTAACTACGCAGTCGATCGCACCATTCGTCCTACCAAAATGAACGTGGACGATGTGCAACGTCTGTCAGTCGCGGTCGTGGTGAATTACAAAACCTTGCCAGATGGCAAACCGTTGACTCCCAGCAACGacc                                         >  1:335347/1‑247 (MQ=255)                                                                                                                                                                                                               ccccTG‑CGAATAACGCGCCAATCAGCCCCCCTCCG‑GCAAATAAAAATAA‑CCGCCAGCAGCAGGCGAGCACCACCAGCAATAGTGGGCCGCGTAGCACACAGCGGAATGAAACCAGTAACTACGAAGTCGATCGCACCATTCGTCATACCAAAATGAACGTGGGCGATGTGCAACGTCTGTCAGTCGCGGTCGTGGTGAATTACAAAACCTTGCCAGATGGCAAACCGTTGCCTCTCAGCAACGAACAGATg                                    <  1:493973/249‑1 (MQ=255)                                                                                                                                                                                                                           accgcgCAAAACAGCACGCCCCCG‑ACAACAAAAAATAA‑CCGCCAGCAGCAGGCGAGCACCACCAGCAATAGTGGGCCGCGTAGCACACAGCGGAATGAAACCAGTAACTACGAAGTCGATCGCACCATTCGTCATACCAAAATGAACGTGGGCGATGTGCAACGTCTGTCAGTCGCGGTCGTGGTGAATTACAAAACCTTGCCAGATGGCAAACCGTTGCCTCTCAGCAACGAACAGATGAAGCAAATTGa                         <  2:164662/249‑1 (MQ=255)                                                                                                                                                                                                                              gcgcaaaccAGCCCGCCCCCG‑GAAAATAAAAAAAA‑CCGCCAGCAGCAGGCGAGCACCACCAGCAATAGTGGGCCGCGTAGCACACAGCGGAATGAAACCAGTAACTACGAAGTCGATCGCACCATTCGTCATACCAAAATGAACGTGGGCGATGTGCAACGTCTGTCAGTCGCGGTCGTGGTGAATTACAAAACCTTGCCAGATGGCAAACCGTTGCCTCTCAGCAACGAACAGATGAAGCAAATTGAAGa                      <  1:414205/243‑1 (MQ=255)                                                                                                                                                                                                                               cgcCAATCAGCACGCCTCCG‑GCAAATCAAAATAA‑CCGCCAGCAGCAGGCGAGCCCCACCAGCAATAGTGGGCCGCGTAGCACACAGCGGAATGAAACCAGTAACTACGAAGTCGATCGCACCATTCGTCATACCAAAATGAACGTGGGCGATGTGCAACGTCTGTCAGTCGCGGTCGTGGTGAATTACAAAACCTTGCCAGATGGCAAACCGTTGCCTCTCAGCAACGAACAGATGAAGCAAATTGa                         >  1:104642/1‑247 (MQ=255)                                                                                                                                                                                                                                    aTCAGCACGCCTCCG‑GCAAATCAAAATAA‑CCGCCAGCAGCAGGCGAGCACCACCAGCAATAGTGGGCCGCGTAGCACACAGCGGAATGAAACCAGTAACTACGAAGTCGATCGCACCATTCGTCATACCAAAATGAACGTGGGCGATGTGCAACGTCTGTCAGTCGCGGTCGTGGTGAATTACAAAACCTTGCCAGATGGCAAACCGTTGCCTCTCAGCAACGAACAGATGAAGCAAATTGAAGATCTGAc                >  2:425905/1‑251 (MQ=255)                                                                                                                                                                                                                                      cggcccGCCTTCG‑GCAAACCAAAAAAT‑CCGCCAGCAGCAGGCGAGCACCACCAGCAATAGTGGGCCGCGTAGCACACAGCGGAATGAAACCAGTAACTACGAAGTCGATCGCACCATTCGTCATACCAAAATGAACGTGGGCGATGTGCAACGTCTGTCAGTCGCGGTCGTGGTGAATTACAAAACCTTGCCAGATGGCAAACCGTTGCCTCTCAGCAACTAACAGATGAAGCAAATTGAAGATCTGAccc              <  1:99418/246‑1 (MQ=255)                                                                                                                                                                                                                                       aGCACTCCTCAG‑GCAAATAAAAATAG‑CCGCCAGCAGCAGGCGCGAACCACCAGCAATCGTGGGCCCCGTAGCACACAGCGGAATGAAACCAGTACCTACGAAGTCGATCGCAAAATTCGTCATACCAAAATGAACGTGGGCGATGAGCAACGTCTGTCAGTCGCGGTCGTGGTGAATTACAAAACCTTGCCAGATGGCAAACCGTTGCCTCTCAGCAACGAACAGATGAAGCAAATTGAAGATCTGACCcg             >  2:478961/1‑251 (MQ=255)                                                                                                                                                                                                                                                  g‑gCAAATCAAAATAA‑CCGCCAGCAGCAGGCGAGCACCACCAGCAATAGTGGGCCGCGTAGCACACAGCGGAATGAAACCAGTAACTACGAAGTCGATCGCACCATTCGTCATACCAAAATGAACGTGGGCGATGTGCAACGTCTGTCAGTCGCGGTCGTGGTGAATTACAAAACCTTGCCAGATGGCAAACCGTTGCCTCTCAGCAACGAACAGATGAAGCAAATTGAAGATCTGACCCGCGAGGCGATgg  >  1:374005/1‑251 (MQ=255)                                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                     GTCGCCTATTGTTGGTAACGGTAATATTCACGCCCAGGTTACGGCGCAGCTGGACTTCGCCAGTAAAGAACAAACGGAAGAACAGTATCGCCCTAACGGTGATGAATCTCATGCGGCGCTTCGTTCACGCCAGCTTAATGAGAGCGAGCAAAGCGGTTCCGG‑TTATCCGG‑GCGGCGTACCGGGGG‑CGTTGTCGAATCAACCGGCACCTG‑CGAATAACGCGCCAATCAGCACGCCTCCG‑GCAAATCAAAATAA‑CCGCCAGCAGCAGGCGAGCACCACCAGCAATAGTGGGCCGCGTAGCACACAGCGGAATGAAACCAGTAACTACGAAGTCGATCGCACCATTCGTCATACCAAAATGAACGTGGGCGATGTGCAACGTCTGTCAGTCGCGGTCGTGGTGAATTACAAAACCTTGCCAGATGGCAAACCGTTGCCTCTCAGCAACGAACAGATGAAGCAAATTGAAGATCTGACCCGCGAGGCGATGG  >  NC_000913/2013978‑2014465

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑