| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,361,765 | 0 | G | T | 25.4% | 98.7 / 10.5 | 63 | A216S (GCG→TCG) | puuD | gamma‑glutamyl‑gamma‑aminobutyrate hydrolase |
| Reads supporting (aligned to +/- strand): ref base G (11/36); new base T (15/1); total (26/37) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.95e-07 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.58e-01 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GAACAGCCTGAATTGCTGGAACATCGGGAAGATCCCGAACTGCCGGTGGAACAGCAATATGCACCGTCGCATGAAGTTCAGGTTGAAGAGGGGGGATTACTGTCTGCGTTGTTACCTGAATGTAGCAACTTTTGGGTAAACTCTCTACATGGACAAGGGGCGAAGGTCGTTAGCCCACGGTTGCGTGTTGAAGCTCGCTCGCCGGATGGCTTAGTTGAGGCGGTTA‑G‑CGTCATCAATCATCCTTTTGCGCTGGGCGT‑ACAGTGGCACCCGGAATGGAACAGTAGCGAGTACGCGCTTTCGCGTATATTGTTCGAGGGCTTTATCACCGCTTGTCAGCACCATATCGCTGAAAAACAGCGACTCTGACCACTACAGTTTAAGGAAATGCAAATATGAGTGATGAGGGACTGGCGCCAGGAAAACGCTTGTCGGAAATCCGCCAGCAGCAGGGGCTTTCACAACGTCGTGCCGCCGAACTCTCCGGGCTGACTC > NC_000913/1361519‑1362010 | gAACAGCCTGAATTGCTGGAACCTCGGGAAGCTCCCGAACTGCCGGTGGAACAGCAATATGCACCGTCGCATGAAGTTCAGGTTGAAGAGGGGGGATTACTGTCTGCGTTGTTACCTGAATGTAGCAACTTTTGGGTAAACTCTCTACATGGACAAGGGGCGAAGGTCGTTAGCCCACGGTTGCGTGTTGAAGCTCGCTCGCCGGATGGCTTAGTTGAGGCGGTTT‑G‑CCTGATCAATCATCCTTTTTCGCt > 2:424261/1‑251 (MQ=255)gAACAGCCTGAATTGCTGGAACATCGGGAAGATCCCGAACTGCCGGTGGAACAGCAATATGCACCGTCGCATGAAGTTCAGGTTGAAGAGGGGGGATTACTGTCTGCGTTGTTACCTGAATGTAGCAACTTTTGGGTAAACTCTCTACATGGACAAGGGGCGAAGGTCGTTAGCCCACGGTTGCGTGTTGAAGCTCGCTCGCCGGATGGCTTAGTTGAGGCGGTTA‑G‑CGTCATCAATCATCCTTTTGCGCt < 1:275950/251‑1 (MQ=255) cTGAATTGCTGGAACATCGGGAAGATCCCGAACTGCCGGTGGAACAGCAATATGCACCGTCGCATGAAGTTCAGGTTGAAGAGGGGGGATTACTGTCTGCGTTGTTACCTGAATGTAGCAACTTTTGGGTAAACTCTCTACATGGACAAGGGGCGAAGGTCGTTAGCCCACGGTTGCGTGTTGAAGCTCGCTCGCCGGATGGCTTAGTTGAGGCGGTTT‑G‑CGTCCTCAATCATTCTTTTTCGCTGGGCgg > 2:245531/1‑249 (MQ=255) tCGGGAAGATCCCGAACTGCCGGTGGAACAGCAATATGCACCGTCGCATGAAGTTCAGGTTGAAGAGGGGGGATTACTGTCTGCGTTGTTACCTGAATGTAGCAACTTTTGGGTAAACTCTCTACATGGACAAGGGGCGAAGGTCGTTAGCCCACGGTTGCGTGTTGAAGCTCGCTCGCCGGATGGCTTAGTTGAGGCGGCTA‑G‑CGGCCTCAATCATCCTTTTTCGCTGGGCGT‑ACATTGGCCCCCgggat > 2:134834/1‑248 (MQ=255) aaGATCCCGAACTGCCGGTGGAACAGCAATATGCACCGGCGCATGAAGTTCAGGTTGACGAGGGGGGATTACTGTCTGCGTTGTTACCTGAATGTAGCAACTTTTGGGTAAACTCTCTACATGGACAAGGGGCGAAGGCCGTTAGCCCACGGTTGAGTGTTGAAGCTCGCTCGCCGGATGGCTTAGTTGAGGCGGTTAAG‑C‑TCCTCAATCCACCTTTTGCGCTGGGCGG‑ACAGTGGGACCCGGacaggga > 2:192916/1‑244 (MQ=255) aGATCCCGAACTGCCGGTGGAACAGCAATATGCACCGTCGCATGAAGTTCAGCTTGAAGAGGGGGGATTACTGTCTGCGTTGTTACCTGAATGTAGCAACTTTTGGGTAAACTCTCTACATGGACAAGGGGCGAAGGTCGTTAGCCCACGGTTGCGTATTGAAGCTCGCTCGCCGGCTGGCTTAATTGAGGCGGTTA‑G‑GGCCATCCATCATCCTTTTGTGACGTGAGT‑TCAGTGGTACCCGGAATGGAAc > 1:302429/1‑250 (MQ=255) tCCCGAACTGCCGGTGGAACAGCAATATGCACCGTCGCATGAAGTTCAGGTTGAAGAGGGGGGATTACTGTCTGCGTTGTTACCTGAATGTAGCAACTTTTGGGTAAACTCTCTACATGGACAAGGGGCGAAGGTCGTTAGCCCACGGTTGCGTGTTGAAGCTCGCTCGCCGGATGGCTTAGTTGAGGCGGTTT‑G‑CGTCCTCAATCATCCTTTTTCCCTGGGCGT‑ACAGTGGCACCCGGAATTGaaactg > 1:401856/1‑246 (MQ=255) tCCCGAACTGCCGGTGGAACAGCAATATGCACCGTCGCATGAAGTTCAGGTTGAAGAGGGGGGATTACTGTCTGCGTTGTTACCTGAATGTAGCAACTTTTGGGTAAACTCTCTACATGGACAAGGGGCGAAGGTCGTTAGCCCACGGTTGCGTGTTGAAGCTCGCTCGCCGGATGGCTTAGTTGAGGCGGTTA‑G‑CGTCCTCAATCCTCCTTTTTCGCTGGGCGT‑ACAGTGGCCACCGGAATGGagaagt > 1:73950/1‑245 (MQ=255) gAACTGCCGGTGGAACAGCAATATGCACCGTCGCATGAAGTTCAGGTTGAAGAGGGGGGATTACTGTCTGCGTTGTTACCTGAATGTAGCAACTTTTGGGTAAACTCTCTACATGGACAAGGGGCGAAGGTCGTTAGCCCACGGTTGCGTGTTGAAGCTCGCTCGCCGGATGGCTTAGTTGAGGCGGTTT‑G‑CGGCATCAATCATCCTTTTGTGCTGGGGGTTAC‑GTGGGACCCGGGATGGAACAGTAGCgg > 2:225992/1‑250 (MQ=255) cGGTGGAACAGCAATATGCACCGTCGCATGAAGTTCAGGTTGAAGAGGGGGGATTACTGTCTGCGTTGTTACCTGAATGTAGCAACTTTTGGGTAAACTCTCTACATGGACAAGGGGCGAAGGTCGTTAGCCCACGGTTGCGTGTTGAAGCTCGCTCGCCGGATGGCTTAGTTGAGGCGGTTA‑G‑CGTCATCAATCATCCTTTTGCGCTGGGCGT‑ACAGTGGCACCCGGAATGGAACAGTAGCGAGTAcgcg < 2:393492/251‑1 (MQ=255) ggAACAGCAATATGCACCGTCGCATGAAGTTCAGGTTGAAGAGGGGGGATTACTGTCTGCGTTGTTACCTGAATGTAGCAACTTTTGGGTAAACTCTCTACATGGACAAGGGGCGAAGGTCGTTAGCCCACGGTTGCGTGTTGAAGCTCGCTCGCCGGATGGCTTAGTTGAGGCGGTTA‑G‑CGTCATCAATCATCCTTTTGCGCTGGGCGT‑ACAGTGGCACCCGGAATGGAACAGTAGCGAGTACGCGCtt < 1:432208/250‑1 (MQ=255) gCAATATGCACCGTCGCATGAAGTTCAGGTTGAAGAGGGGGGATTACTGTCTGCGTTGTTACCTGAATGTAGCAACTTTTGGGTAAACTCTCTACATGGACAAGGGGCGAAGGTCGTTAGCCCACGGTTGCGTGTTGAAGCTCGCTCGCCGGATTGCTTAGTTGAGGCGGTTA‑G‑CGTCATCAATCATCCTTTTGCGCTGGGCGT‑ACAGTGGCACCCGGAATGGAACAGTATCGAGTACGCGCTTTCGCGta < 2:303531/251‑1 (MQ=255) gCACCGTCGCATTAAGTTCAGGTTGAAGAGGGGGGATTACTGGCTGCGTTGTTACCTGAATGTAGCAACTTTTGGGTAAACTCTCTACATGGACAAGGGGCGAAGGTCGTTAGCCCACGGTTGCGTGTTGAAGCTCGCGCGCCGGATGGCTTAGTTGAGGCGGTTA‑G‑CGTCATCAATCATCCTTTTGCGCTGGGCGT‑ACAGTGGCACCCGGAATTGAACAGTAGCGAGTACGCGCTTTCGCGTATATTGt < 2:334774/250‑1 (MQ=255) gCATGAAGTTCAGGTTGAAGAGGGGGGATTACTGTCTGCGTTGTTACCTGAATGTAGCAACTTTTGGGTAAACTCTCTACATGGACAAGGGGCGAAGGTCGTTAGCCCACGGTTGCGTGTTGAAGCTCGCTCGCCGGATGGCTTAGTTGAGGCGGTTT‑G‑CGGCATCCATACTCCTTTTGCGCTCGGGGG‑ACAGTGGCACCCGGGATGGGACCGGAGCGAGTACGCGCTTTCGCCGATATTTTTTGAGGGCt > 2:402093/1‑251 (MQ=255) cATGAAGTTCAGGTTGAAGAGGGGGGATTACTGTCTGCGTTGTTACCTGAATGTAGCAACTTTTGGGTAAACTCTCTACATGGACAAGGGGCGAAGGTCGTTAGCCCACGGTTGCGTGTTGAAGCTCGCTCGCCGGATGGCTTAGTTGAGGCGGTTA‑G‑CGTCCTCAATTCTCCTTTTTCGCTGGGCGT‑ACAGTGGCACCCGGAATGGAACCGAAGCGGGTACGCGCTTTCGCGTATATTGTTCGAGGGCtt > 1:136659/1‑251 (MQ=255) aGTTCAGGTTGAAGAGGGGGGATTACTGTCTGCGTTGTTACCTGAATGTAGCAACTTTTGGGTAAACTCTCTACATGGACAAGGGGCGAAGGTCGTTAGCCCACGGTTGCGTGTTGAAGCTCGCTCGCCGGATGGCTTAGTTGAGGCGGTTA‑G‑GGGCATCAATCACCCTTTTTCGCTGGGCGT‑ACAGTGGGACCCCGAATGGGAAAGGATCGGGGACGCGCTTTCGAGTATATTGTTTGAGGGGTTTATc > 1:201677/1‑250 (MQ=255) gTTCAGGTTGAAGAGGGGTGAGTACTGTCTGCTTTGTTCCCTGAATGTAGCAACTTTTGGGTAAACTCTCTACAGGGACAAGGGGCGAAGGTCGTTAGCCTGCGGTTGCGTGTTGAAGCTCGCTCGCCGGATGGCTTAGTTGTGGCGGTTA‑G‑CGTCATCAATCATCCTTTTGCGCTGGGCGT‑ACAGGGGCTCCCGGAATGGAACAGTATCGAGTACGCGCTTTCGCGGATATTGTTCGAGGGCTTTATctc < 2:281523/251‑3 (MQ=255) ggTTGAAGAGGGGGGATTACTGTCTGCGTTGTTACCTGAATGTAGCAACTTTTGGGTAAACTCTCTACATGGACAAGGGGCGAAGGTCGTTAGCCCACGGTTGCGTGTTGAAGCTCGCTCGCCGGATGGCTTAGTTGAGGCGGTTA‑G‑CGTCATCAATCATCCTTTTGCGCTGGGCGT‑ACAGTGGCACCCGGAATGGAACAGTAGCGAGTACGCGCTTTCGCGTATATTGTTCGAGGGCTTTATCTCCGCtt < 1:52480/251‑1 (MQ=255) ggTTGAAGAGGGGGGATTACTGTCTGCGTTGTTACCTGAATGTAGCAACTTTTGGGTAAACTCTCTACATGGACAAGGGGCGAAGGTCGTTAGCCCACGGTTGCGTGTTGAAGCTCGCTCGCCGGATGGCTTAGTTGAGGCGGTTA‑G‑CGTCATCAATCATCCTTTTGCGCTGGGCGT‑ACAGTGGCACCCGGAATGGAACAGTAGCGAGTACGCGCTTTCGCGTATATTGTTCGAGGGCTTTATCACCGCtt < 2:177468/251‑1 (MQ=255) gTTGAAGAGGGGGGATTACTGTCTGCGTTGTTACCTGAATGTAGCAACTTTTGGGTAAACTCTCTACATGGACAAGGGGCGAAGGTCGTTAGCCCACGGTTGCGTGTTGAAGCTCGCTCGCCGGATGGCTTAGTTGAGGCGGTTA‑G‑CGTCATTAATCATCCTTTTGCGCTGGGGGT‑AACGTGGCCCCCCGAATGGAACAGTAGCGAGGACGCGCTTTCGCGTGTATTGTTCGAGGGGTTTTTTACCGCttt > 2:404410/1‑250 (MQ=255) ggggATTACTGTCTGCGTTGTTACCTGAATGTAGCAACTTTTGGGTAAACTCTCTACATGGACAAGGGGCGAAGGTCGTTAGCCCACGGTTGCGTGTTGAAGCTCGCTCGCCGGATGGCTTAGTTGAGGCGGTTA‑G‑CGTCATCAATCATCCTTTTGCGCTGGGCGT‑ACAGTGGCACCCGGAATGGAACAGTAGCGAGTACGCGCTTTCGCGTATATTGTTCGAGGGCTTTATCACCGCTTGTCAGCACCat < 1:87303/251‑1 (MQ=255) cTGCGTTGTTAACTGAATGTAGCAACTTTTGGGTAAACTCTCTACATGGCCAAGGGGCGAAGGTCGTTAGCCCACGGTTGCGTGTTGAAGCTCGCTCGCCGGATGGCTTAGTTGAGGCGGTTA‑G‑CGTCATCAATCATCCTTTTTCGCTGGGCGG‑ACAGTGGCACCCGGAATGGAAACGTAGCGAGGAAGCGCTTTCGCGTATATTGTTCGAGGGATTTATCACCGCTTGTccgaaccctctagctgaaaa > 1:412038/1‑231 (MQ=255) gttACCTGAATGTAGCAACTTTTGGGTAAACTCTCTACATGGACAAGGGGCGAAGGTCGTTAGCCCACGGTTGCGTGTTGAAGCTCGCTCGCCGGATGGCTTAGTTGAGGCGGTTA‑G‑CGTCATCAATCATCCTTTTGCGCTGGGCGT‑ACAGTGGCACCCGGAATGGAACAGTAGCGAGTACGCGCTTTCGCGTATATTGTTCGAGGGCTTTATCACCGCTTGTCAGCACCATATCGCTGAAAAACAGCGAc > 1:89604/1‑251 (MQ=255) tACCTGAATGTAGCAACTTTTGGGTAAACTCTCTACATGGACAAGGGGCGAAGGTCGTTAGCCCACGGTTGCGTGTTGAAGCTCGCTCGCCGGATGGCTTAGTTGAGGCGGTTA‑G‑CGTCATCAATCATCCTTTTTCGCTGGGCGG‑ACAGCGGGACCCGGAATGGGACAGTAGCGAGTACGCGCTTTCGCGGATATTTTTTGCGGGCTTTATTACCCCTTGTCAGCACCCTATCGCTGAAAAACAGCGActc > 1:413248/1‑251 (MQ=255) gAATGTAGCAACTTTTGGGTAAACTCTCTACATGGACAAGGGGCGAAGGTCGTTAGCCCACGGTTGCGTGTTGAAGCTCGCTCGCCGGATGGCTTAGTTGAGGCGGTTA‑A‑CGTCATCAATCCTCCTTTTGGGCTGGGGGG‑ACAAAGGGCCCCGGGATGGAACACGAACGCGTACGGGCTTTCGAGGATATTGTTCGCGGGCTTTATCACCGCTTATCCGCACCATATCGCTGgaaaaaaacggctcttacc > 1:219081/1‑237 (MQ=255) aaTGTAGCAACTTTTGGGTAAACTCTCTACATGGACAAGGGGCGAAGGTCGTTAGCCCACGGTTGCGTGTTGAAGCTCGCTCGCCGGATGGCTTAGTTGAGGCGGTTA‑G‑CGTCAGCAATCATCCTTTTGCGCTGGGCGT‑ACAGTGGCACCCGGAATGGAACAGTAGCGAGTACGCGCTTTCGCGTATATTGTTCGAGGGCTTTATCACCGCTTGTCAGCACCATATCGCTGAAAAACAGCGACTCTGACCa < 1:173569/251‑1 (MQ=255) aGCAACTTTTGGGTAAACTCTCTACATGGACAAGGGGCGAAGGTCGTTAGCCCACGGTTGCGTGTTGAAGCTCGCTCGCCGGATGGCTTAGTTGAGGCGGTTA‑G‑CGTCATCAATCATCCTTTTGCGCTGGGCGT‑ACAGTGGCACCCGGAATGGAACAGTAGCGAGTACGCGCTTTCGCGTATATTGTTCGAGGGCTTTATCACCGCTTGTCAGCACCATATCGCTGAAAAACAGCGACTCTGACCACTACa < 1:254104/251‑1 (MQ=255) aGCAACTTTTGGGTAAACTCTCTACATGGACAAGGGGCGAAGGTCGTTAGCCCACGGTTGCGTGTTGAAGCTCGCTCGCCGGATGGCTTAGTTGAGGCGGTTA‑G‑CGGCATCAATCATCCTTTTGCGCTGGGGGT‑ACAGGGGCACCCGGAATGGAACAGTAGCGGGTACGCGCCTTCGCGGATATTGTTCGAGGGCTTTATTACCGCTTGTTAGCCCCATATCGCTGAAAAACAACGCCTCTGACCACTAcc > 1:389346/1‑250 (MQ=255) aCTTTTGGGTAAACTCTCTACATGGACAAGGGGCGAAGGTCGTTAGCCCACGGTTGCGTGTTGAAGCTCGCTCGCCGGATGGCTTAGTTGAGGCGGTTA‑G‑CGTCATCAATCATCCTTTTGCGCTGGGCGT‑ACAGTGGCACCCGGAATGTAACAGTAGCGAGTACGCGCTTTCGCGTATATTGTTCGAGGGCTTTATCACCGCTTGTCAGCACCATATCGCTGAAAAACAGCGACTCTGACCACTACAGttt < 2:307100/251‑1 (MQ=255) gggTAAACTCTCTACATGGACAAGGGGCGAAGGTCGTTAGCCCACGGTTGCGTGTTGAAGCTCGCTCGCCGGATGGCTTAGTTGAGGCGGTTA‑G‑CGTCATCAATCATCCTTTTGCGCTGGGCGT‑ACAGTGGCACCCGGAATGGAACAGTAGCGAGTACGCGCTTTCGCGTATATTGTTCGAGGGCTTTATCACCGCTTGTCAGCACCATATCGCTGAAAAACAGCGACTCTGACCACTACAGTTTAAGGa < 2:10151/250‑1 (MQ=255) gTAAACTCTCTACATGGACAAGGGGCGAAGGTCGTTAGCCCACGGTTGCGTGTTGAAGCTCGCTCGCCGGATGGCTTAGTTGAGGCGGTTA‑G‑CGTCATCAATCATCCTTTTGCGCTGGGCGT‑ACAGTGGCACCCGGAATGGAACAGTAGCGAGTACGCGCTTTCGCGTATATTGTTCGAGGGCTTTATCACCGCTTGTCAGCTCCATATCGCTGAAAAACAGCGACTCTGACCACTACAGTTTAAGGAAAt < 1:465859/251‑1 (MQ=255) aaaCTCTCTACATGGACAAGGGGCGAAGGTCGTTAGCCCACGGTTGCGTGTTGAAGCTCGCTCGCCGGATGGCTTAGTTGAGGCGGTTA‑G‑CGTCATCAATCATCCTTTTGCGCTGGGCGT‑ACAGTGGCACCCGGAATGGAACAGTAGCGAGTACGCGCTTTCGCGTATATTGTTCGAGGGCTTTATCACCGCTTGTCAGCACCATATCGCTGAAAAACAGCGACTCTGACCACTACAGTTTAAGGAAATg < 1:136394/250‑1 (MQ=255) aaaCTCGCTACATGGACAAGGGGCGAAGGTCGTTAGCCCACGGTTGCGTGTTGAAGCTCGCTCGCCGGATGGCTTAGTTGAGGCGGTTA‑G‑CGTCATCAATCATCCTTTTGCGCTGGGCGT‑ACAGTGGCACCCGGAATGGAACAGTAGCGAGTACGCGCTTTCGCGTATATTGTTCGAGGGCTTTATCACCGCTTGTCAGCACCATATCGCTGAAAAACAGCGACTCTGACCACTACAGTTTAAGGAAATGc < 2:389346/251‑1 (MQ=255) ctACATGGACAAGGGGCGAAGGTCGTTAGCCCACGGTTGCGTGTTGAAGCTCGCTCGCCGGATGGCTTAGTTGAGGCGTTTA‑G‑CGTCATCAATCATCCTTTTGCGCTGGGCGT‑ACAGTGGCACCCGGAATGGAACAGTAGCGAGTACGCGCTTTCGCGTATATTGTGCGAGGGCTTTATCACCGCTTGTCAGCACCATATCGCTGAAAAACAGCGACTCTGACCACTACAGTTTAAGGAAATGCAAata < 1:237902/249‑1 (MQ=255) ctACATGGACAAGGGGCGAAGGTCGTTAGCCCACGGTTGCGTGTTGAAGCTCGCTCGCCGGATGGCTTAGTTGAGGCGGTTG‑G‑CGTCCTCAATCCACCTTTTTCGCTGGGCGT‑ACCGTTGCCCCCGGAATAGGACCGTAACACGTCCACGCTTTGGCCGCTATTGTTCGCGGGCTTTATCACCGCTTGTCAGCCCCATATCGCTGCAAAACAGCGGCCCTGACCCCTACCCTTTAAGGAAATTCACATATg > 1:273602/1‑251 (MQ=255) ctACATGGACAAGGGGCGAAGGTCGTTAGCCCACGGTTGCGTGTTGAAGCTCGCTCGCCGGATGGCTTAGTTGAGGCGGTTA‑G‑CGTCATCAATCATCCTTTTGCGCTGGGCGT‑ACAGTGGCACCCGGAATGGAACAGTAGCGAGTACGCGCTTTCGCGTATATTGTTCGAGGGCTTTATCACCGCTTGTCAGCTCCATATCGCTTAAAAACAGCGACTCGGACCACTACAGTTTAAGGAAATGCAAat < 2:319531/248‑1 (MQ=255) aCATGGACAAGGGGCGAAGGTCGTTAGCCCACGGTTGCGTGTTGAAGCTCGCTCGCCGGATGGCTTAGTTGAGGCGGTTA‑G‑CGTCATCAATCATCCTTTTGCGCTGGGCGT‑ACAGTGGCACCCGGAATGGAACAGTAGCGAGTACGCGCTTTCGCGTATATTGTTCGAGGGCTTTATCACCGCTTGTCAGCACCATATCGCTGAAAAACAGCGACTCTGACCACTACAGTTTAAGGAAATGCAAATATg < 2:161335/249‑1 (MQ=255) gACAAGGGGCGAAGGTCGTTAGCCCACGGTTGCGTGTTGAAGCTCGCTCGCCGGATGGCTTAGTTGAGGCGGTTA‑G‑CGTCATCAATCATCCTTTTGCGCTGGGCGT‑ACAGTGGCACCCGGAATGGAACAGTAGCGAGTACGCGCTTTCGCGTATATTGTTCGAGGGCTTTATCACCGCTTGTCAGCACCATATCGCTGAAAAACAGCGACTCTGACCACTACAGTTTAAGGAAATGCAAATATGAGtgatg < 2:201677/251‑1 (MQ=255) aCAAGGGGCGAAGGTCGTTAGCCCACGGTTGCGTGTTGAAGCTCGCTCGCCGGATGGCTTAGTTGAGGCGGTTA‑G‑GGGCATCAAACATCCTTTTGCGCTGGGCGT‑ACAGTGGCACCCCGGATGGAACAGTAGCGAGGACGCGCTTTTGGGGTTATTGTTCGAGGGCGTTATCCCCCCTTTTCAGCCCCCTATTCCTGAAAAACAAAGGCCCTTACCACTACAGTTTAAAGAAATGCAAATATGAggggtgg > 2:272078/1‑245 (MQ=255) aaGGGGCGAAGGTCGTTAGCCCACGGTTGCGTGTTGAAGCTCGCTCGCCGGATGGCTTAGTTGAGGCGGTTA‑G‑CGTCATCAATCATCCTTTTTCGCTGGGCGG‑ACAGTGGCCCCCGGAAAGGAACAgg > 1:262577/1‑127 (MQ=255) aaGGGGCGAAGGTCGTTAGCCCACGGTTGCGTGTTGAAGCTCGCTCGCCGGATGGCTTAGTTGAGGCGGTTA‑G‑CGTCATCAATCATCCTTTTTCGCTGGGCGG‑ACAGTGGCACCCGGAATGGAACAGGAGCGAGTACGCGCTTTCGCGGATATTGTTCGAGGGCTTTATTACCGCTTTTCAGCACCCTTTCGCTGGAAAACAACGGCTCTGACCCCTACAGTTTAAAGAAATGCAAATATGAGtggatagg > 1:5015/1‑245 (MQ=255) aaGGGGCGAAGGTCGTTAGCCCACGGTTGCGTGTTGAAGCTCGCTCGCCGGATGGCTTAGTTGAGGCGGTTA‑G‑CGTCATCAATCATCCTTTTGCGCTGGGCGT‑ACAGTGGCACCCGGAATGGAACAGt < 2:262577/128‑1 (MQ=255) aGGGGCGAAGGTCGTTAGCCCACGGTTGCGTGTTGAAGCTCGCTCGCCGGATGGCTTAGTTGAGGCGGTTA‑G‑CGTCATCAATCATCCTTTTTCGCTGGGGGG‑ACAGGGGGCCCCGGAATGGAAAAGTAGCGGGTACGCGCTTTCTCGGATATTGGTTGAGGGCTTTATCACCGCTTGGCAGCACCCAATTGCTGGAAAACAAGGGCTTTTACCACTACAGGTTAAGGAAATTCAAATAAGAGTGATGAggg > 1:206995/1‑251 (MQ=255) gggCGAAGGTCGTTAGCCCACGGTTGCGTGTTGAAGCTCGCTCGCCGGATGGCTTAGTTGAGGCGGTTA‑G‑CGTCATCAATCATCCTTTTGCGCTGGGCGT‑ACAGTGGCACCCGGAATGGAACAGTAGCGAGTACGCGCTTTCGCGTATATTGTTCGAGGGCTTTATCACCGCTTGTCAGCACCATATCGCTGAAAAACAGCGACTCTGACCACTACAGTTTAAGGAAATGCAAATATGAGTGATGAGGGAc < 1:326500/251‑1 (MQ=255) ggCGAAGGTCGTTAGCCCACGGTTGCGTGTTGAAGCTCGCTCGCCGGATGGCTTAGTTGAGGCGGTTA‑G‑CGTCATCAATCATCCTTTTGCGCTGGGCGT‑ACAGTGGCACCCGGAATGGAACAGTAGCGAGTACGCGCTTTCGCGTATATTGTTCGAGGGCTTTATCACCGCTTGTCAGCACCATATCGCTGAAAAACAGCGACTCTGACCACTACAGTTTAAGGAAATGCAAATATGAGTGATGAGGGACt < 1:369201/251‑1 (MQ=255) ggCGAAGGTCGTTAGCCCACGGTTGCGTGTTGAAGCTCGCTCGCCGGATGGCTTAGTTGAGGCGGTTA‑G‑CGTCATCAATCATCCTTTTGCGCTGGGCGT‑ACAGTGGCACCCGGAATGGAACAGTAGCGAGTACGCGCTTTCGCGTATATTGTTCGAGGGCTTTATCACCGCTTGTCAGCACCATAGCGCTGAAAAACAGCGACTCTGACCACTACAGTTTAAGGAAATGCAAATATGAGTGATGAGGGACt < 2:177463/251‑1 (MQ=255) gAAGGTCGTTAGCCCACGGTTGCGTGTTGAAGCTCGCTCGCCGGATGGCTTAGTTGAGGCGGTTA‑G‑CGTCATCAATCATCCTTTTGCGCTGGGCGT‑ACAGTGGCACCCGGAATGGAACAGTAGCGAGTACGCGCTTTCGCGTATATTGTTCGAGGGCTTTATCACCGCTTGTCAGCACCATATCGCTGAAAAACAGCGACGCTGACCACTACAGTTTAAGGAAATGCAAATATGAGTGATGAGGGACTGgc < 2:73950/251‑1 (MQ=255) cGTTAGCCCACGGTTGCGTGTTGAAGCTCGCTCGCCGGATGGCTTAGTTGAGGCGGTTA‑A‑CGTCATCAATCATCCTTTTTCGCTGGGCGG‑ACAGTGGCCCCCGGAATGGAACAGTAGCGAGGACGCGCTTTCGCGGATATTTTTCGAGGGCTTTATCACCGCTTGTCAGCACCATATCGCTGAAAAACAACGACTCTGGCCACTACAGTTTAAGGAAATGCAAATATGAGTGATGAGGGACTGGCGcccgg > 1:374860/1‑248 (MQ=255) tAGCCCACGGTTGCGTGTTGAAGCTCGCTCGCCGGATGGCTTAGTTGAGGCGGTTA‑G‑CGTCAGCAATCATCCTTTTGCGCTGGGCGT‑ACAGTGGCACCCGGAATGGAACAGTAGCGAGTACGCGCTTTCGCGTATATTGTTCGAGGGCTTTATCACCGCTTGTCAGCACCATATCGCTGAAAAACAGCGACTCTGACCACTACAGTTTAAGGAAATGCAAATATGAGTGATGAGGGACTGGCGCCAGGaa < 2:219081/250‑1 (MQ=255) aGCCCACGGTTGCGTGTTGAAGCTCGCTCGCCGGATGGCTTAGTTGAGGCGGTTA‑G‑CGTCATCAATCATCCTTTTGCGCTGGGCGT‑ACAGTGGCACCCGGAATGGAACAGTAGCGAGTACGCGCTTTCGCGTATATTGTTCGAGGGCTTTATCACCGCTTGTCAGCACCATATCGCTGAAAAACAGCGACTCTGACCACTACAGTTTAAGGAAATGCAAATATGAGTGATGAGGGACTGGCGCCAGGaaaa < 2:89604/251‑1 (MQ=255) tGAAGGTCGCTCGCCGGATGGCTTAGTTGAGGCGGTTT‑GAGGTAAT‑ACTCATCCTTTTTAGCTGGGGGG‑AACGTGGGACCAGGGATGGAACAAGATCGAGTACGCGCTTTTGCGTATATTTTTCGGGGGCTTTCTCACCCATTTGCAGCACAATATCGCTGGAAAACAGAGCCTCTGACCAACACCGGTTAAGGAAATGTAAAAATAGGTGATGAGGGACAGGCGGCATGAGAAAGCTTGTCggtataacg > 2:489165/1‑244 (MQ=255) gAAGCTCGCTCGCCGGATGGCTTAGTTGAGGCGGTTA‑G‑CGTCATCAATCATCCTTTTGCGCTGGGCGT‑ACAGTGGCACCCGGAATGGAACAGTAGCGAGTACGCGCTTTCGCGTATATTGTTCGAGGGCTTTATCACCGCTTGTCAGCACCATATCGCTGAAAAACAGCGACTCTGACCACTACAGTTTAAGGAAATGCAAATATGAGTGATGAGGGACTGGCGCCAGGAAAACGCTTGTCGGAAATCCg < 2:136659/250‑1 (MQ=255) cgcCGGATGGCTTAGTTGAGGCGGTTA‑G‑CGTCATCAATCATCCTTTTGCGCTGGGCGT‑ACAGTGGCACCCGGAATGGAACAGTAGCGAGTACGCGCTTTCGCGTATATTGTTCGAGGGCTTTATCACCGCTTGTCAGCACCATATCGCTGAAAAACAGCGACTCTGACCACTACAGTTTAAGGAAATGCAAATATGAGTGATGAGGGACTGGCGCCAGGAAAACGCTTGTCGGAAATCCGCCAGCAGCAgg < 2:401856/251‑1 (MQ=255) gATGGCTTAGTTGAGGCGGTTA‑G‑CGTCATCAATCATCCTTTTGCGCTGGGCGT‑ACAGTGGCACCCGGAATGGAACAGTAGCGAGTACGCGCTTTCGCGTATATTGTTCGAGGGCTTTATCACCGCTTGTCAGCACCATATCGCTGAAAAACAGCGACTCTGACCACTACAGTTTAAGGAAATGCAAATATGAGTGATGAGGGACTGGCGCCAGGAAAACGCTTGTCGGAAATCCGCCAGCAGCAGGGGCtt < 1:70788/251‑1 (MQ=255) aTGGCTTAGTTGAGGCGGTTA‑G‑CGTCATCAATCATCCTTTTGCGCTGGGCGT‑ACAGTGGCACCCGGAATGGAACAGTAGCGAGTACGCGCTTTCGCGTATATTGTTCGAGGGCTTTATCACCGCTTGTCAGCACCATATCGCTGAAAAACAGCGACTCTGACCACTACAGTTTAAGGAAATGCAAATATGAGTGATGAGGGACTGGCGCCAGGAAAACGCTTGTCGGAAATCCGCCAGCAGCAGGGGCttt < 1:272078/251‑1 (MQ=255) tAGTTGAGGCGGTTA‑G‑CGTCATCAATCATCCTTTTGCGCTGGGCGT‑ACAGTGGCACCCGGAATGGAACAGTAGCGAGTACGCGCTTTCGCGTATATTGTTCGAGGGCTTAATCACCGCTTGTCAGCACCATATCGCTGAAAAACAGCGACTCTGACCACTACAGTTTAAGGAAATGCAAATATGAGTGATGAGGGACTGGCGCCAGGAAAACGCTTGTCGGAAATCCGCCAGCAGCAGGGGCTTTCACaa < 1:77443/250‑1 (MQ=255) gTTGAGGCGGTTA‑G‑CGTCATCAATCAGCCTTTTGCGCTGGGCGT‑ACAGTGGCACCCGGAATGGAACAGTAGCGAGTACGCGCTTTCGCGTATATTGTTCGAGGGCTTTATCACCGCTTGTCAGCACCATATCGCTGAAAAACAGCGACTCTGACCACTACAGTGTAAGGAAATGCAAATATGAGTGAGGAGGGACTGGCGCCAGGAAAACGCTTGTCGGAAATCCGCCAGCAGCAGGGGCTTTCACAAcgt < 2:169668/251‑1 (MQ=255) gAGGCGGTTA‑G‑CGTCATCAATCATCCTTTTGCGCTGGGCGT‑ACAGTGGCACCCGGAATGGAACAGTAGCGAGTACGCGCTTTCGCGTATATTGTTCGAGGGCTTTATCACCGCTTGTCAGCACCATATCGCTGAAAAACAGCGACTCTGACCACTACAGTTTAAGGAAATGCAAATATGAGTGATGAGGGACTGGCGCCAGGAAAACGCTTGTCGGAAATCCGCCAGCAGCAGGGGCTTTCACAAcgtcgt < 2:417473/251‑1 (MQ=255) a‑G‑CGTCATCAATCATCCTTTTGCGCTGGGCGT‑ACAGTGGCACCCGGAATGGAACAGTAGCGAGTACGCGCTTTCGCGTATATTGTTCGAGGGCTTTATCACCGCTTGTCAGCACCATATCGCTGAAAAACAGCGACTCTGACCACTACAGTTTAAGGAAATGCAAATATGAGTGATGAGGGACTGGCGCCAGGAAAACGCTTGTCGGAAATCCGCCAGCAGCAGGGGCTTTCACAACGTCGTGCCGCCGa > 2:185679/1‑250 (MQ=255) caatcaTCCTTTTTCGCTGGGCGT‑ACAGTGGCACCCGGAATGGAACAGTAGCGAGTACGCGCTTTCGCGTATATTGTTCGAGGGCTTTATCACCGCTTGTCAGCACCATATCGCTGAAAAACAGCGACTCTGACCACTACAGTTTAAGGAAATGCAAATATGAGTGATGAGGGACTGGCGCCAGGAAAACGCTTGTCGGAAATCCGCCAGCAGCAGGGGCTTTCACAACGTCGTGCCGCCGAACTCTCCgg < 2:413248/251‑1 (MQ=255) tcCTTTTGCGCTGGGCGT‑ACAGTGGCACCCGGCATGGAACAGTAGCGAGTACGCGCTTTCGCGTATATTGTTCGAGGGCTTTATCACAGCTTGTCAGCACCATATCGCTGACAAACAGCGACTCTGACCACTACAGTTTAAGGAAATGCAAATATGAGTGATGAGGGACTGGCGCCAGGAAAACGCTTGTCGGAAATCCGCCAGCAGCAGGGGCTTTCACAACGTCGTGCCGCCGAACTCTCCGGGCTGAc > 1:434347/1‑251 (MQ=255) ccTTGTGCGCTGGGCGT‑ACAGTGGCACCCGGAATGGAACAGTAGCGAGTACGCGCTTTCGCGTATATTGTTCGAGGGCTTTATCACCGCTTGTCAGCACCATATCGCTGAAAAACAGCGACTCTGACCACTACAGTTTAAGGAAATGCAAATATGAGTGATTAGGGACTGGCGCCAGGAAAACGCTTGTCGGAAATCCGCCAGCAGCAGGGGCTTTCACAACGTCGTGCCGCCGAACTCTCCGGGCTGACt < 2:246807/251‑1 (MQ=255) tttGCGCTGGGCGT‑ACAGTGGCACCCGGAATGGAACAGTAGCGAGTACGCGCTTTCGCGTATATTGTTCGAGGGCTTTATCACCGCTTGTCAGCACCATATCGCTGAAAAACAGCGACTCTGACCACTACAGTTTAAGGAAATGCAAATATGAGTGATGAGGGACTGGCGCCAGGAAAACGCTTGTCGGAAATCCGCCAGCAGCAGGGGCTTTCACAACGTCGTGCCGCCGAACTCTCCGGGCTGACTc < 1:402093/249‑1 (MQ=255) | GAACAGCCTGAATTGCTGGAACATCGGGAAGATCCCGAACTGCCGGTGGAACAGCAATATGCACCGTCGCATGAAGTTCAGGTTGAAGAGGGGGGATTACTGTCTGCGTTGTTACCTGAATGTAGCAACTTTTGGGTAAACTCTCTACATGGACAAGGGGCGAAGGTCGTTAGCCCACGGTTGCGTGTTGAAGCTCGCTCGCCGGATGGCTTAGTTGAGGCGGTTA‑G‑CGTCATCAATCATCCTTTTGCGCTGGGCGT‑ACAGTGGCACCCGGAATGGAACAGTAGCGAGTACGCGCTTTCGCGTATATTGTTCGAGGGCTTTATCACCGCTTGTCAGCACCATATCGCTGAAAAACAGCGACTCTGACCACTACAGTTTAAGGAAATGCAAATATGAGTGATGAGGGACTGGCGCCAGGAAAACGCTTGTCGGAAATCCGCCAGCAGCAGGGGCTTTCACAACGTCGTGCCGCCGAACTCTCCGGGCTGACTC > NC_000913/1361519‑1362010 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |