Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	NC_000913	1,907,563	IS1 (–) +9 bp	coding (21‑29/144 nt)	yobF ←	DUF2527 domain‑containing protein YobF

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	1907563 =	1 (0.020)	47 (0.990)	43/484	0.2	97.8%	coding (29/144 nt)	yobF	DUF2527 domain‑containing protein YobF
	?	NC_000913	= 1979270	NA (NA)					noncoding (1/768 nt)	IS1	repeat region
*	?	NC_000913	= 1907571	1 (0.020)	41 (0.860)	34/486	0.4	97.5%	coding (21/144 nt)	yobF	DUF2527 domain‑containing protein YobF
	?	NC_000913	1978503 =	NA (NA)					noncoding (768/768 nt)	IS1	repeat region

ACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCC  >  NC_000913/1979163‑1979271                                                                                                            |  accaccCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCAccc  >  1:57628/1‑109 (MQ=11) accaccCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCAccc  <  2:57628/109‑1 (MQ=11)                                                                                                            |  ACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCC  >  NC_000913/1979163‑1979271

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.