Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,207,549	Δ1 bp	coding (179/606 nt)	ttdB →	L(+)‑tartrate dehydratase subunit beta

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,207,549	0	G	.	100.0%	257.3 / NA	56	coding (179/606 nt)	ttdB	L(+)‑tartrate dehydratase subunit beta
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base . (32/24);  total (32/24)

CGCGGCACGCTGCTGGTTCATGCCGATCTCACCTTTGAAAATCTGTCTCACACCCGGAGCGCGTTATGAAAAAGATCCTGACAACCCCGATCAAAGCTGAAGATCTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACG‑TTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTGGCCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCTACATGTGATTTTCCCGGCAGGCTGCGCGGTGCTGGCGGCAACCCAGGTGGAAGAGATTGAAGAAGTGCACT  >  NC_000913/3207306‑3207797                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                         cgcgGCACGCTGCTGGTTCATGCCGATCTCACCTTTGAAAATCTATCTAACACCCGGAGCGCGTTATGAAAAAGATCATGACAACCCCGATCAAAGCTGAAGATCTGAAAGATATTAGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACATGCCGCGACG‑TTTGTAACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCCCGCTG‑CCCCATCg                                                                                                                                                                                                                                                  >  1:301435/1‑251 (MQ=255)  gcgGCACGCTGCTGGTTCATGCCGATCTCACCTTTGAAAATCTGTCGCACACCCGGAGCGCGTTATGAAAAAGATCCTGACAACCCCGATCAAAGCTGAAGATCTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACG‑TTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGt                                                                                                                                                                                                                                                 <  2:228006/251‑1 (MQ=255)               ggtgcATGCCGATCTCACCTTTGAAAATCTGTCTCACACCCGGTGCGCTTTATGAAAAAGATCCTGACAACCCCGATCAAAGCTGAAGATCTGCATGTTATTCGCTCCGGCGATGTGATCTACCTGCCCGTTACGCTGGTGACCTGCCGCGACG‑TTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGc                                                                                                                                                                                                                                    <  1:500287/247‑1 (MQ=255)               ggTTCATGCCGATCTCACCTTTGAAAATCTGTCTCACCCCCGGAGCGCGTTATGAAAAAGATCCTGACAACCCCGATCAAAGCTGAAGATCTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACG‑TTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACgg                                                                                                                                                                                                                                     <  1:229507/250‑1 (MQ=255)                gTTCATGCCGATCTCACCTTTGAAAATCTGTCTCACACCCGGACCGCGTTATGAAACAGATCCTGACCACCCAGCTCAAACCTGACGCTCTGCAAGATATTCGCGGCGGCGATGTGCTCTACCTGACCGGTACGCAGATGGCCTGACGAGACG‑TTTGTCAACGCCGTTGGATCGAACTGAAACGTCCGATCCCTTACGGTCTCACAGGCAGAGCGATTTTCCACGCTG‑CCCAATCGTGCGCAAAAACGGCg                                                                                                                                                                                                                                   >  2:161523/1‑251 (MQ=255)                              cACCTTTGAAAATCTGTCTCACACCCGGAGCGCGTTAGGAAAAAGATCCTGACACCCCCGAACAAAGCTGAAGATCTGCAAGATATTCGCGTCGGCGATGTAATCTACCTGACCGGTACGCTGGTGCCCTGCCGCGACG‑TTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGgag                                                                                                                                                                                                                        <  2:89632/248‑1 (MQ=255)                                ccTTTGAAAATCTGTCTCACACCCGGAGCTCGTTATGAAAAAGATCTTGACAACCCCGACCAAAGCTGAAGATCTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACG‑TTTGTCCCCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGt                                                                                                                                                                                                                   <  1:462090/251‑1 (MQ=255)                                    tGAAAATCTGTCTCACCACCGGAGTGGGTTATGAAAAAGATCCTGTCAACCCCGATCAAAGCTGAAGATCGTCAAGATATTCTCGTCGGCGATGTGATCTACCTGCCCGGTACGCTGTTGCCCTGCCGCGACT‑TTGGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGGTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGtctc                                                                                                                                                                                                                <  2:301999/250‑1 (MQ=255)                                             gTCTCACACCCGGAGCGCGTTATGAAAAAGATCCTGACAACCCCGATCAAAGCTGAAGATCTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACG‑TTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCg                                                                                                                                                                                                      >  1:104777/1‑251 (MQ=255)                                               ctcACAACCGGAGCGCGTTATGAAAAAGATCCTGACAACCCCGATCAAAGCTGAAGATCTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACG‑TTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGAc                                                                                                                                                                                                    >  2:463561/1‑251 (MQ=255)                                                 cacaCCCGGAGCGCGTTATGAAAAAGATCCTTACACCCCCGATCAAAGCTGAAGACCTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACG‑TTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGAc                                                                                                                                                                                                    <  2:394968/249‑1 (MQ=255)                                                   cacCCGGAGCGCGTTATGAAAAAGATCCTGACAACCCCGATCAAAGCTGAAGATCTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACG‑TTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAAcc                                                                                                                                                                                                >  1:136531/1‑251 (MQ=255)                                                    acCCGGAGCGCGTTATGAAAAAGATCCTGACAACCCCGATCAAAGCTGCAGATCTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACTGGTACGCTGGTGACCTGCCGCGACG‑TTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGTCCCGACAAcc                                                                                                                                                                                                >  2:377158/1‑250 (MQ=255)                                                     cccGGAGCGGGTTAGGAAACAGTGCCTGACACCCCCGACCAAAGTTAAAGATCTGCAAGATATCCGCGCCTGCGATGTGATCTCCCTACCCGGTACGCTGCTGACCTCCCGCGACG‑TTTGTCCCCGCCGTTTGATCGAACTGAAACGGCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCCCGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAg                                                                                                                                                                                              <  2:424571/251‑1 (MQ=255)                                                                  aTGAAAAAGATCCTGACAACCCCGATCAAAGCTGAAGATCTGCAAGATATTCGCGTCGGCGATGGGATCTACCTGACCGGTACGCTGGTGACCTGCCGCTACG‑TTTGTCTCCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACACCCAGTATGCGTATGGaa                                                                                                                                                                                 <  1:17273/251‑1 (MQ=255)                                                                      aaaagcgcctcaCAACCCCGAACAAATCTGAAGATCTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACG‑TTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGta                                                                                                                                                                             <  2:359274/240‑2 (MQ=255)                                                                          gATCCTGACAACCCCGATCAAAGCTGAAGATCTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACG‑TTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTg                                                                                                                                                                          >  1:86815/1‑250 (MQ=255)                                                                           aTCCTGACAACCCCGATCAAAGCTGAAGATCTGCAAGATATTCGCGTCGGCGATGTGATCTACCGGACCGGTACGCTGGTGACCTGCCGCGACG‑TTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGaa                                                                                                                                                                        <  1:463561/251‑1 (MQ=255)                                                                           aTCCTGACAAACCCGATCAAAGCTGAAGATCTGCAAGATATTCGCGTAGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACG‑TTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGa                                                                                                                                                                         >  2:325538/1‑250 (MQ=255)                                                                                 aaaacgccGATCAAAGCTGCAGATCTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACG‑TTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTGACGATCTCAACGGCAAAGCGATTTTCCAAGCTG‑CCCCATCGTGCGCACAAACGGCGACAAATGGGAGATGGTCTCCGTCGGACCGACAACCAGTATGCGGATGGAAAGtgtggtacgtga                                                                                                                                                                   >  2:91904/7‑239 (MQ=255)                                                                                          aTCAAAGCTGAAGATTTGCAATAGTTTCGCGGCGGCGATGTGATCGACCTAACCGGTACGCTGGTGACCTGCCGCGACG‑TTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGTCAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAg                                                                                                                                                         <  2:385292/251‑1 (MQ=255)                                                                                                    aaGATCTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACG‑TTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCg                                                                                                                                               >  1:343005/1‑251 (MQ=255)                                                                                                       aTCTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACG‑TTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTg                                                                                                                                             >  1:75111/1‑250 (MQ=255)                                                                                                             aaGATATTCGCGTCGGCGCTGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACG‑TTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CGCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCg                                                                                                                                                                                                      >  2:343503/1‑187 (MQ=255)                                                                                                             aaGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACG‑TTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CGCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCg                                                                                                                                                                                                      <  1:343503/187‑1 (MQ=255)                                                                                                                 tatTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACG‑TTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTgggggt                                                                                                                                  >  2:457270/1‑251 (MQ=255)                                                                                                                       cgTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACG‑TTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCa                                                                                                                             >  1:111435/1‑250 (MQ=255)                                                                                                                           ggCGCTGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACG‑TTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGATACTGGTGGTTGGCAAAgg                                                                                                                         >  1:391136/1‑250 (MQ=255)                                                                                                                             gtaTGTGTTCTACCTGCCCGCTACGAGGGTGACCGGCCGCG‑CGCTTTGTCACCGCCGTTTGATCGAACGTAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTGCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGTCAACCAGTATGCGTATGGAAAGGTTTGAACGTGATTTTATTGAGCAGACCGGCGTTAAACTGGGGGTTGGCAAAggtgg                                                                                                                      <  2:421130/249‑1 (MQ=255)                                                                                                                                 gtgATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACG‑TTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGAGGTATg                                                                                                                  >  2:174309/1‑251 (MQ=255)                                                                                                                                     ccTACCTGTCCGGTACGCTGTGGTCCTCCCGCGACG‑TTTGACACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTAGGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGc                                                                                                              <  1:287938/250‑1 (MQ=255)                                                                                                                                           tGACCGGTACGCTGGTAACCTGCCGCGACG‑TTTGTCACCGCCGTTTGATCGAACTGAAACGCCCGATACATTACGATCTCACCGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAACCGGCGACAAATGGGCGATGGTCTCCGTCCGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTTAATTTATTGAGCAGACCGGCGTGAAACTGGTGGATGTCAAAGGAGGAATGGGGCCGCTg                                                                                                         >  1:216457/1‑250 (MQ=255)                                                                                                                                             ccctgTACGCTGGTGCCTTGCCGCGTCG‑TTTGTCTCCGCTGTTTGAGCGAACTTAAACGTCCGATCCCTTTCGATCTCAACGTCAAAGCGATTTTCCACGCTG‑CCCCATCTTGCGCAAAAACGTCTACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGAcc                                                                                                      <  1:483634/247‑1 (MQ=255)                                                                                                                                                      cTGGTGACCTGCCGCGACG‑TTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGAAAACCAGTATGCGTATGGa                                                                                                                                                                                  >  1:320642/1‑166 (MQ=255)                                                                                                                                                             ccTGCCGCGACG‑GTTGTCACCGCCGTTTGATCGAACGGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAATGCTGCCAGa                                                                                      <  1:341633/251‑1 (MQ=255)                                                                                                                                                              cTGCCGCGACG‑TTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGa                                                                                      >  1:228383/1‑250 (MQ=255)                                                                                                                                                                  cgcgACG‑TTTGTCAACGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGGGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTc                                                                                 >  2:309063/1‑251 (MQ=255)                                                                                                                                                                    cgACG‑TTCGACACCGCCGTTTGCTCGAACCGAAACGTCCGACACCTTACGCTCTCAACGGCAAAGCGATTCTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCACTCGGCCCGACAACCAGTATGCGTATAGAAAGATTTGACGGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGTTTGGCAAAGGAGGGCTGGGGGCGTCGACCGACGACAGCTGCCAGAAATTCa                                                                                >  2:74781/1‑250 (MQ=255)                                                                                                                                                                        g‑TTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGgcg                                                                           <  2:136531/251‑1 (MQ=255)                                                                                                                                                                        g‑TTTGTCACCGACGTATGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGTTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGgcg                                                                           <  1:436391/251‑1 (MQ=255)                                                                                                                                                                                cccgccgTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACGGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCTACAtg                                                                    <  1:307869/250‑1 (MQ=255)                                                                                                                                                                                                  tGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACt                                                                                                                                        >  1:136990/1‑165 (MQ=255)                                                                                                                                                                                                  tGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACt                                                                                                                                        <  2:136990/165‑1 (MQ=255)                                                                                                                                                                                                   gAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCTACATGTGATTTTCCCGGCAGGCTg                                                 >  1:202686/1‑251 (MQ=255)                                                                                                                                                                                                        gTCCGATCCCTTACGATCTCACCGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCTACATGTGATTTTCCCGGCAGGCTGCGCgg                                            >  1:174459/1‑251 (MQ=255)                                                                                                                                                                                                            gATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCTACATGTGATTTTCCCGGCAGGCTGCGCGGTGCt                                        >  2:35488/1‑251 (MQ=255)                                                                                                                                                                                                            gATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCTACATGTGATTTTCCCGGCAGGCTGCGCGGTGCt                                        >  1:86455/1‑251 (MQ=255)                                                                                                                                                                                                             aTCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCTACAGGTGATTTTCCCGGCAGGCTGCGCGTTGCTg                                       <  2:75111/251‑1 (MQ=255)                                                                                                                                                                                                                ccTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCTACATGTGATTTTCCCGGCAGGCTGCGCGGTGCTggcg                                    >  2:142218/1‑251 (MQ=255)                                                                                                                                                                                                                      gATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCACAAACGGCGACAAATGGGAGCTGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCTACATGTGATTTTCCCGGCAGGCCGCGCGGTGCTGGCGGCAAcc                              >  2:348301/1‑251 (MQ=255)                                                                                                                                                                                                                           cAACGGCAAAGCGATTTTCCACGCTG‑CCCCAGCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTGTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTAGGGGGCCGCTGACCGAAGAAGGCTGCCAGAAAGTCAAGGCGCTACATGTGATTTTCCCGGCAGGCTGCGCGGTGCTGGCGGCAAc                               <  1:98495/245‑1 (MQ=255)                                                                                                                                                                                                                              cGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCTACATGTGATTTTCCCGGCAGGCTGCGCGGTGCTGGCGGCAACCCAGGTgg                       >  1:339012/1‑250 (MQ=255)                                                                                                                                                                                                                               ggCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGCCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGCAGGCTGCCAGAAATTCAAGGCGCTACATGTGATTTTCCCGGCAGGCTGCGCGGAGCTGGCGGCAACCCAGGTGGa                      >  1:52713/1‑250 (MQ=255)                                                                                                                                                                                                                                 cAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCTACATGTGATTTTCCCGGCAGGCTGCGCGGTGCTGGCGGCAACCCAGGTGGAaga                   <  1:309063/251‑1 (MQ=255)                                                                                                                                                                                                                                  aaaGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCTACATGTGATTTTCCCGGCAGGCTGCGCGGTGCTGGCGGCAACCCAGGTGGAag                    >  1:145312/1‑249 (MQ=255)                                                                                                                                                                                                                                   aaGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCTACATGTGATTTTCCCGGCAGGCTGCGCGGTGCTGGCGGCAACCCAGGTGGAag                    >  1:258182/1‑248 (MQ=255)                                                                                                                                                                                                                                                   ctgCCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGAGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCTACATGTGATTTTCCCGGCAGGCTGCGCGGTGCTGGCGGCAACCCAGGGGGAAGAGATTGAAGAAGTGCACt  >  2:386621/3‑251 (MQ=255)                                                                                                                                                                                                                                                   ctgCCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTAGGAACGACAACCAGTATGCGTATGGAAAGTTTTGAAAGTGAATTTATTGAGCAGACCGGAGTGAAAATGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACAGAAGAAGGCTGCCAGAAATTCAAGGCGCTACATGTGATGTACCCGGCAGGCTGGGCGGAGCTGGCGGCAACCCAGGTGGAAGGGAAGGAAGAAGTGCACt  >  1:409755/3‑251 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                         CGCGGCACGCTGCTGGTTCATGCCGATCTCACCTTTGAAAATCTGTCTCACACCCGGAGCGCGTTATGAAAAAGATCCTGACAACCCCGATCAAAGCTGAAGATCTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACG‑TTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTGGCCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCTACATGTGATTTTCCCGGCAGGCTGCGCGGTGCTGGCGGCAACCCAGGTGGAAGAGATTGAAGAAGTGCACT  >  NC_000913/3207306‑3207797

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑