Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,111,259	Δ1 bp	coding (718/900 nt)	rfbD ←	dTDP‑4‑dehydrorhamnose reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,111,259	0	G	.	100.0%	128.4 / NA	27	coding (718/900 nt)	rfbD	dTDP‑4‑dehydrorhamnose reductase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base . (14/13);  total (14/13)

CATCTTGCTCCACCATCACGAACAAGATGCAAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGTTGGTACTGCGTTGAGCTTGTTGAGTGCAAGGGGAATGCCTGCTTTGCGCGCCTCTTCAAAAACCAGCGCAGCATAATCGTACCAGGTTGTGGTACCACTGGCTACCAAATGGTACAAGCCTGCGACATCCGGTTTATTCAGTGCGACACGAATGGCATGTGCTGTACAATCAGCCAGCAGTTCAGCACCTGTTGGCGCACCAAACTGATCGTTAATAACCGCTAATTCTTCACGCTCTTTTGCCAGACGTAACATCGTTTTGGCGAAGTTATTTCCTTTTC  >  NC_000913/2111039‑2111507                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                         cATCTTGCTCCACCATCACGAACAAGATGCAAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGATGTTGTTGGTACTGCGTTGAGCTTGTTGAGTGCAAGGG‑AATGCCTGCTTTGCGCGCCTCTTCAAAAAcc                                                                                                                                                                                                                           >  2:408841/1‑251 (MQ=255)                    gAACAAGATGCAAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGTTGGTACTGCGTTGAGCTTGTTGAGTGCAAGGG‑AATGCCTGCTTTGCGCGCCTCTTCAAAAACCAGCGCAGCATAATCGTAcc                                                                                                                                                                                                        >  2:233516/1‑251 (MQ=255)                                   aCTATTAAAGTGCTTTAGTCGTAAATAATTCAGTGAGCAGTCTTTCCACGCCAACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGTAAGTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGTTGGTAATGCGTTGAGCTTGTTGAGTGCAAGGG‑AATGCCTGCTTTGCGCGCCTCTTCAAAAACCAGCGCAGCATAATCGTACCAGGTTGTGGTTCCAc                                                                                                                                                                                         <  2:365547/251‑1 (MQ=255)                                            ttGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGTTGGTACTGCGTTGAGCTTGTTGAGTGCAAGGG‑AATGCCTGCTTTGCGCGCCTCTTCAAAAACCAGCGCAGCATAATCGTACCAGGTTGTGGTACCACTGGCTAcc                                                                                                                                                                                 >  2:155613/1‑250 (MQ=255)                                                  tAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAATTTTTCTGTGTTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGTTGGTACTGCGTTGAGCTTGTTGAGTGCCAGGG‑AATGCCTGCTTTGCGCGCCTCTTCAAAAACCAGCGCAGCATAATCGTACCCGGTTGTGGTCCCCCTGGCTACCAAATGGt                                                                                                                                                                          >  2:439919/1‑251 (MQ=255)                                                         aaaTAATTCATTGAGCATTCGGTTCACGCCAACCTGCCAGTCATGCAAGACAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGTTGGTACTGCGTTGAGCTTGTTGAGTGCAAGGG‑AATGCCTGCTTTGCGCGCCTCTTCAAAAACCAGCGCAGCATAAGCGTACCAGGTTTTTGTACCACTGGCTACCAAATGGTACAAGcc                                                                                                                                                                   <  1:408841/251‑1 (MQ=255)                                                                   ttGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGTTGGTACTGCGTTGAGCTTGTTGAGTGCAAGGG‑AATGCCTGCTTTGCGCGCCTCTTCAAAAACCAGCGCAGCATAATCGTACCAGGTTGTGGTACCACTGGCTACCAAATGGTACAAGCCTGCGACATcc                                                                                                                                                         >  1:295274/1‑251 (MQ=255)                                                                                 cACGCCAACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGTTGGTACTGCGTTGAGCTTGTTGAGTGCAAGGG‑AATGCCTGCTTTGCGCGCCTCTTCAAAAACCAGCGCAGCATAATCGTACCAGGTTGTGGTACCACTGGCTACCAAATGGTACAAGCCTGCGACATCCGGTTTATTCAGTGc                                                                                                                                           >  1:339078/1‑251 (MQ=255)                                                                                   cGCCAACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGTTGGTACTGCGTTGAGCTTGTTGAGTGCAAGGG‑AATGCCTGCTTTGCGCGCCTCTTCAAAAACCAGCGCAGCATAATCGTACCAGGTTGTGGTACCACTGGCTACCAAATGGTACAAGCCTGCGACATCCGGTTTATTCAGTGCg                                                                                                                                          >  1:154380/1‑250 (MQ=255)                                                                                                          aCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGTTGGTACTGCGTTGAGCTTGTTGAGTGCAAGGG‑AATGCCTGCTTTGCGCGCCTCTTCAAAAACCAGCGCAGCATAATCGTACCAGGTTGTGGTACCACTGGCTACCAAATGGTACAAGCCTGCGACATCCGGTTTATTCAGTGCGACACGAATGGCATGTGCTGTACa                                                                                                                   >  2:94185/1‑250 (MQ=255)                                                                                                             aGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGTTGGTACTGCGTTGAGCTTGTTGAGTGCAAGGG‑AATGCCTGCTTTGCGCGCCTCTTCAAAAACCAGCGCAGCATAATCGTACCAGGTTGTGGTACCACTGGCTACCAAATGGTACAAGCCTGCGACATCCGGTTTATTCAGTGCGACACGAATGGCATGTTCTGTACAATca                                                                                                               <  1:112294/251‑1 (MQ=255)                                                                                                                                   tttCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGAATAGGCTGTTGTTGGTACTGCGTTGAGCTTGTTGAGTGCAAGGG‑AATGCCTGCTTTGCGCGCCTCTTCAAAAACCAGCGCAGCATAATCGTACCAGGTTGTGGTACCACTAGCTACCAAATGGTACCAGACTGCGACATCCGGTTTATTCAGTGCGACACGCAAGGCATGTGCTGTACCATCAGCCAGCAGTTCAGCAACTGTTg                                                                                         >  1:297189/1‑251 (MQ=255)                                                                                                                                    ttCTGTATTAAGGCGAGAGTTATGTGGACGACGCGCTGGTGTAGGATAGGCTGTTGTTGGTACTGCGTTGAGCTTGTTGAGTGCAAGGG‑AATGCCTGCTTTGCGCGCCTCTTCAAAAACCAGCGCAGCATAATCGTACCAGGTTGTGGTACCACTGGCTACCAAATGGTACAAGCCTGCGACATCCGGTTTATTCAGTGCGACACGAATGGCATGTGCTGTACAATCAGCCAGCAGTTCAGCACCTGTTgg                                                                                        >  1:403988/1‑251 (MQ=255)                                                                                                                                              aGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGTTGGTACTGCGTTGAGCTTGTTGAGTGCAAGGG‑AATGCCTGCTTTGCGCGCCTCTTCAAAAACCAGCGCAGCATAATCGTACCAGGTTGTGGTACCACTGGCTACCAAATGGTACAAGCCTGCGACATCCGGTTTATTAAGTGCGACACGAATGGCATGTGCTGTACAATCAGCCAGCAGTTCAGCCCCTGTTGGCGCACCAAAc                                                                              >  1:12690/1‑251 (MQ=255)                                                                                                                                                       ttATGGGGACGACGAGCTGGTGCAGGAGAGGATGCTGTTGGTACTGCGGTGAGCGTGTTGAGTGCAAGGG‑AAAGCCTGCTTTGCGCGCCTCTGCAAAAACGAGCGCAGCATAATCGTACCAGGATGTGGTACCACTGGCTACCAAATGGTACAAGCCTGCGACATCCGGTTTATTCAGTGCGCAACGAAAGGCATGTGCCGTACAATCAGCCAGCAGATCACGACCTGTAGGCGCACCaaaatgcacggt                                                                      >  2:148122/1‑241 (MQ=255)                                                                                                                                                        tATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGGTGGTACTGCGTTGAGCTTGTTGAGTGCAAGGG‑AATGCCTGCTTTGCGCGCCTCGTCAAAAACCAGCGCAGCATAATCGTACCAGGTTGTGGTACCACTGGCTACCAAATGGTACAAGCCTGCGACATCCGGTTTATTCAGTGCGACACGAATGGCATGTGCTGTACAATCAGCCAGCAGTTCAGCGCCTGTTGGCGCACCAAACTGATCGTta                                                                     <  2:337635/250‑1 (MQ=255)                                                                                                                                                           gtgGACGACGAGCTGGTGTAGGATAGGCTGTTGTTGGTACTGCGTTGAGCTTGTTGAGTGCAAGGG‑AATGCCTGCTTTGCGCGCCTCTTCAAAAACCAGCGCAGCATAATCGTACCAGGTTGTGGTACCACTGGCTACCAAATGGTACAAGCCTGCGACATCCGGTTTATTCAGTGCGACACGAATGGCATGTGCTGTACAATCAGCCAGCAGTTCAGCACCTGTTGGCGCACCAAACTGATCGTtaataa                                                                 <  2:125380/251‑1 (MQ=255)                                                                                                                                                                             tAGGATAGGCTGTTGTTGGTACTGCGTTGAGCTTGTTGAGTGCAAGGG‑AATGCCTGCTTTGCGCGCCTCTTCAAAAACCAGCGCAGCATAATCGTACCAGGTTGTGGTACCACTGGCTACCAAATGGTACAAGCCTGCGACATCCGGTTTATTCAGTGCGACACGAATGGCATGTGCTGTACAATCAGCCAGCAGTTCAGCACCt                                                                                             <  2:408880/205‑1 (MQ=255)                                                                                                                                                                                 aTAGGCTGTTGTTGGTACTGCGTTGAGCTTGTTGAGTGCAAGGG‑AATGCCTGCTTTGCGAGCCTCTTCAAAAACCAGCGCAGCATAATCGTACCAGGTTGTGGTACCACTGGCTACCAAATGGTACAAGCCTGCGACATCCGGTTTATTCAGTGCGACACGAATGGCATGTGCTGTAAAATCAGCCAGCAGTTCAGCACCTGTTGGCGCACCAAACTGATCGTTAATAACCGCTAATTCTTCACGCTCttt                                           >  1:56947/1‑251 (MQ=255)                                                                                                                                                                                   aGGCTGTTGTTGGTACTGCGTTGAGCTTGTTGAGTGCAAGGG‑AATGCCTGCTTTGCGCGCCTCTTCAAAAACCAGCGCAGCATAATCGTACCAGGTTGGGGTACCACTGGCTACCAAATGGTACAAGCCTGCGACATCCGGTTTATTCAGTGCGACACGAATGGCATGTGCTGTACAATCAGCCAGCAGTTCAGCACCTGTTGGCGCACCAAACTGATCGTTAATAACCGCTAATTCTTCACGCTCTTTTg                                         <  1:94753/251‑1 (MQ=255)                                                                                                                                                                                    ggCTGTTGTTGGTACTGCGTTGAGCTTGTTGAGTGCAAGGG‑AATGCCTGCTTTGCGCGCCTCTTCAAAAACCAGCGCAGCATAATCGTACCAGGTTGTGGTACCACTGGCTACCAAATGGTACAAGCCTGCGACATCCGGTTTATTCAGTGCGACACGAATGGCATGTGCTGTACAATCAGCCAGCAGTTCAGCACCTGTTGGCGCACCAAACTGATCGTTAATAACCGCTAATTCTTCACGCTCTTTTGc                                        >  1:35571/1‑251 (MQ=255)                                                                                                                                                                                        gttgttGGTACTGCGTTGAGCTTGTTGATTGCAAGGG‑AATGCCTGCTTGGCGCGCCTCTTCAAAAACCAGCGCAGCATAATCGTACCAGGTTGTGGTACCACTGGCTACCAAATGGTACAAGCCTGCGACATCCGGTTTATTCAGTGCGACACGAATGGCATGTGCTGTACAATCAGCCAGCAGTTCAGCACCTGTTGGCGCACCAAACTGATCGTTAATAACCGCTAATTCTTCACGCTCTTTTGCCAg                                     <  2:149862/250‑1 (MQ=255)                                                                                                                                                                                              gggaccgctttGAGCTTGTTGAGTGCAATGG‑AATGCCTGCTTTGCGCGCCTCTGCAAAAACCAGCGCAGCATAATCGTACCAGGTGGTGGTACCACTGGCTACCAAATGTGACAAGCCTGCGACATCCGGTTTATTCAGTGCGACACGAATGGCATGTGCTGTACAATCAGCCAGCAGTCCAGCACCTGTTTGCGCACCAAACTGATCGTTAATCACCGCTAATTCTTCACGCTCTTGTGCCAGACGTAAc                              <  1:321654/242‑1 (MQ=255)                                                                                                                                                                                              gggaCTGCGTTGAGCTTGTTGAGTGCAAGGG‑AATGCCTGCGTTGCGCTCCTCTTCAAAAACCAGCGCAGCATAATCGTACCAGGTTGTGGTACCACTGGCTACCAAATGGTACAAGCCTGCGACATCCGGTTTATTCAGTGCGACACGAATGGCATGTGCTGTACAATCAGCCAGCAGTTCAGCACCTGTTGGCGCACCAAACTGATCGTTAATAACCGCTAATTCTTCACGCTCTTTTGCCAGACGTAAc                              <  2:9255/248‑1 (MQ=255)                                                                                                                                                                                                    gCGTTGAGCTTGTTGAGTGCAAGGG‑AATGCCTGCTTTGCGCGCCTCTTCAAAAACCAGCGCAGCATAATCGTACCAGGTTGTGGTACCACTGGCTACCAAATGGTACAAGCCTGCGACATCCGGTTTATTCAGTGCGACACGAATGGCATGTGCTGTACAATCAGCCAGCAGTTCGGCACCTGTTGGCGCACCAAACTGATCGTTAATAACCGCTAATTCTTCACGCTCTTTTGCCAGACGTAACATCGt                         <  1:138273/250‑1 (MQ=255)                                                                                                                                                                                                              tgttgAGTGCAAGGG‑AATGCCTGCTTTGCGCGCCTCTTCAAAAACCAGCGCAGCATAATCGTACCAGGTTGTGGTACCACTGGCTACCAAATGGTACAAGCCTGCGACATCCGGTTTATTCAGTGCGACACGAATGGCATGTGCTGTACAATCAGCCAGCAGTTCAGCACCTGTTGGCGCACCAAACTGATCGTTAATAACCGCTAATTCTTCACGCTCTTTTGCCAGACGTAACATCGTTTTGGCGAAg               <  1:155613/250‑1 (MQ=255)                                                                                                                                                                                                              tgttgAGTGCAAGGG‑AATGCCGGCTTTGCGCGCCTCTTCAAAAACCAGCGCAGCATAATCGTACCAGGTTGTGGTACCACTGGCTACCAAATGGTACAAGCCTGCGACATCCGGTTTATTCAGTGCGACACGAATGGCATGTGCTGTACAATCAGCCAGCAGTTCAGCACCTGTTGGCGCACCAAACTGATCGTTAATAACCGCTAATTCTTCACGCTCTTTTGCCAGACGTAACATCGTTTTGGCGaa                <  1:190191/249‑1 (MQ=255)                                                                                                                                                                                                               tgtggagggcaagggAATGCCTGCTTTGCGCGCCTCTTCAAAAACCAGCGCATCATAATCGTACCAGGTTGTGGTACCACTGGCTACCAAATGGGACAAGCCTGCGACATCCGGTTTATGCAGTGCGACACGAATGGCATGTGCTGTACAATCAGCCAGCAGTTCAGCACCTGTTGGCGCACCAAACTGATCGTTAATAACCGCTAATTCTTCACGCTCTTTTGCCAGACGTAACATCGTTTTGGCGAAg               <  1:308464/238‑1 (MQ=255)                                                                                                                                                                                                                           gggAATGCCTGCTTTGCGCGCCTCTTCAAAAACCAGCGCAGCATAATCGTACCAGGTTGTGGTACCACTGGCTACCAAATGGTACAAGCCTGCGACATCCGGTTTATTCAGTGCGACACGAATGGCATGTGCTGTACAATCAGCCAGCAGTTCAGCACCTGTTGGCGCACCAAACTGATCGTTAATAACCGCTAATTCTTCACGCTCTTTTGCCAGACGTAACATCGTTTTGGCGAAGCTAtttccttttc  >  1:318851/1‑251 (MQ=255)                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                         CATCTTGCTCCACCATCACGAACAAGATGCAAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGTTGGTACTGCGTTGAGCTTGTTGAGTGCAAGGGGAATGCCTGCTTTGCGCGCCTCTTCAAAAACCAGCGCAGCATAATCGTACCAGGTTGTGGTACCACTGGCTACCAAATGGTACAAGCCTGCGACATCCGGTTTATTCAGTGCGACACGAATGGCATGTGCTGTACAATCAGCCAGCAGTTCAGCACCTGTTGGCGCACCAAACTGATCGTTAATAACCGCTAATTCTTCACGCTCTTTTGCCAGACGTAACATCGTTTTGGCGAAGTTATTTCCTTTTC  >  NC_000913/2111039‑2111507

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑