Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,093,346	(T)6→5	coding (471/1359 nt)	dgcT →	putative diguanylate cyclase DgcT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,093,346	0	T	.	100.0%	78.1 / NA	18	coding (471/1359 nt)	dgcT	putative diguanylate cyclase DgcT
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (8/10);  total (8/10)

TAGTTTGGCTTTTCTGAGTTGTTTGATTTACTTTGTTATAACCGTCATTATCATTCAGCAAATTATTGAGGAGCGTTTGACAAGCAGTGTTGTCCAAAATGACATTGCAATCTATTATTTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCAAAAAAATGACTTTGTGCATAAGTTTGTTTTTTGTTTTTGGGGGGCCGATAGTTGCTCACATACTTTCTAGTCATTATGAGAGCTACAATTTACACATTGCCGAGTTAACCAATGAAAATGGTCAGGTGGTTTGGAAAGCCTCATATGTTACCATAATGATTTTCATGTGGTTAACCTTG‑CTTTCGGTTAATCTATACTTTAATGGTTTACGCTATGATATCTGGA  >  NC_000913/1093106‑1093539                                                                                                                                                                                                                                                 |                                                                                                                                                                                                   tAGTTTGGCTTTTCTGAGTTGTTTGATTTACTTTGTTATAACCGTCATTATCATTCAGCAAATTATTGAGGAGCGTTTGACAAGCAGTGTTGTCCAAAATGACATTGCAATCTATTATTTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCAAAAAAATGACTTTGTGCATAAGTTTGTTTTT‑GTTTTTggggg                                                                                                                                                                                         >  1:466922/1‑251 (MQ=255)  agtgtGGCTTTTCTTAGTTGTTTGAGTTACTTTGTTATAACCGTCATTATCATTCAGCAAATTATTGAGGAGCGTTTGACAAGCAGTGTTGTCCAAAATGACATTGCAATCTATTATTTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCAAAAAAATGACTTTGTGCATAAGTTTGTTTTT‑GTTTTTggggg                                                                                                                                                                                         <  1:205167/246‑1 (MQ=255)                          aTTTACATTGTTATAATCGTCATTATCATTACGCAAATTATTGTGGACCGTTTCACAAGCAGTGTTGTCCAAAATCACACTGCAATCTATTATTTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGCGACAACACAAAACAGCGCAGTTTTTTTTCTAAAAAAATGATTTTTAGCATAAGTTTGTTTTT‑GTTTTTGGGGGGCCGATAGTTGCTCACAttctttct                                                                                                                                                                >  1:371834/1‑251 (MQ=255)                                          ccGTCATTATCATTCAGCAAATTATTGAGGAGCGTTTGACAAGCAGTGTTGTCCAAAATGACATTGCAATCTATTATTTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCAAAAAAATGACTTTGTGCATAAGTTTGTTTTT‑GTTTTTGGGGGGCCGATAGTTGCTCACATACTTTCTAGTCATTATGAGAGCt                                                                                                                                                <  2:129840/251‑1 (MQ=255)                                              cATTATCATTCAGCAACTTATTGAGGAGCGTTTGACAAGCAGTGTTGTCCAAAATGACATTGCAATCTATTATTTGTTTCGTCAGATGAGGTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGCAAACACAAAACAGCGCAATGTATTTTCCAAAAAAATGACTTTGTGCATCAGTTTGTTTTT‑GTTTTTGGGGGGCCGATAGTTGCTCAAATACTTTCTAGTCATTATGAGAGCTACa                                                                                                                                             >  2:462942/1‑250 (MQ=255)                                                            aaaTTATTGAGGAGCGTTTGACAAGCAGTGTTGTCCAAAATGACATTGCAATCTATTATTTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCAAAAAAATGACTTTGTGCATAAGTTTGTTTTT‑GTTTTTGGGGGGCCGATAGTTGCTCACATACTTTCTAGTCATTATGAGAGCTACAATTTACACATTGCCg                                                                                                                              >  2:123222/1‑251 (MQ=255)                                                                     aggagCGTTTGACAAGCAGTGTTGTCCAAAATGACATTGCAATCTATTATTTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCAAAAAAATGACTTTGTGCATAAGTTTGTTTTT‑GTTTTTGGGGGGCCGATAGTTGCTCACATACTTTCTAGTCATTATGAGAGCTACAATTTACACATTGCCGAGTTAAcc                                                                                                                      >  1:134895/1‑250 (MQ=255)                                                                                           tgtCCAAAATGACATTGCAATCTATTATTTGTTTCGTCAGATGAGTTTGAGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCAAAAAAATGACTTTGTGCATAAGTTTGTTTTT‑GTTTTTGGGGGGCCGATAGTTGCTCACATCCTTTCTAGTCATTATGAGAGCTACAATTTACACTTTGCCGAGATAACAAATGAAAATGGTCAGGTGGTTTg                                                                                               >  1:213297/1‑251 (MQ=255)                                                                                                   aTGACATTGCAATCTATTATTTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCAAAAAAATGACTTTGTGCATAAGTTTGTTTTT‑GTTTTTGGGGGGCCGATAGTTGCTCACATACTTTCTAGTCATTATGAGAGCTACAATTTACACATTGCCGAGTTAACCAATGAAAATGGTCAGGTGGTTTGGAAAGCCt                                                                                       <  2:58610/251‑1 (MQ=255)                                                                                                        aTTGCAATCTATTATTTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCAAAAAAATGACTTTGTGCATAAGTTTGTTTTT‑GTTTTTGGGGGGCCGATAGTTGCTCACATACTTTCTAGTCATTATGAGAGCTACAATTTACACATTGCCGAGTTAACCAATGAAAACGGTCAGGTGGTTTGCAAAGCCTCatat                                                                                  <  2:197038/251‑1 (MQ=255)                                                                                                             aaTCTATTATTTGTTTCGTCAGATGCGTTTGTGCATATTAATATTTCTGGCATTGTTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCAAAAAAATGACTTTGTGCATAAGTTTGTTTTT‑GTTTTTGGGGGGCCGATAGTTGCTCACATAATTTCTAGTCATTATGAGAGCTACAATTTACACATTGCCGAGTTAACCAATGAAAATGGTCAGGTGGTTTGGACAGCCTCATATGTTAc                                                                             >  2:206145/1‑251 (MQ=255)                                                                                                                                     gAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCAAAAAAATGACTTTGTGCATAAGTTTGTTTTT‑GTTTTTGGGGGGCCGATAGTTGCTCACATACTTTCTAGTCATTATGAGAGCTACAATTTACACATTGCCGAGTTAACCAATGAAAATGGTCAGGTGGTTTGGAAAGCCTCATATGTTACCATAATGATTTTCATGTGGTTAAc                                                     <  2:134895/251‑1 (MQ=255)                                                                                                                                                tatTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCAAAAAAATGACTTTGTGCATAAGTTTGTTTTT‑GTTTTTGGGGGGCCGATAGTTGCTCACATACTTTCTAGTCATTATGAGAGCTACAATTTACACATTGCCGAGTTAACCAATGAAAATGGTCAGGTGGTTTGGAAAGCCTCATATGTTACCATAATGATTTTCATGTGGTTAACCTTG‑CTTTCgg                                         >  2:279267/1‑251 (MQ=255)                                                                                                                                                   tAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCAAAAAAATGACTTTGTGCATAAGTTTGTTTTT‑GTTTTTGGGGGGCCGATAGTTGCTCACATACTTTCTAGTCATTATGAGAGCTACAATTTACACATTGCCGAGTTAACCAATGAAAATGGTCAGGTGGTTTGGAAAGCCTCATATGTTACCATAATGATTTTCATGTGGTTAACCTTG‑CTTTCGGtt                                       <  1:279267/250‑1 (MQ=255)                                                                                                                                                          tgtGGCATTGGTGAATAAAGTTAGAGAAAAAACAAAACAGCGCAATTGATTTTCCAAAAAAATGACTTTGTGCATAAGTTTGTTTTT‑GTTTTGGGGGGAACGATAGTTGCTCACATACTTTCTAGTCATTATTAGAGCTACAATTTACACATTGCAGAGTTAACCAATTAAAATGGTCAGGTGGTTTGGAAAGCCTCATATGTTACCATAATGATTTTCATGTGGTTAAC‑TTGTCTTTCGGTTAATCtct                                <  2:302250/248‑3 (MQ=255)                                                                                                                                                                           aaGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCAAAAAAATGACTTTGTGCATAAGTTTGTTTTT‑GTTTTTGGGGGGCCGATAGTTGCTCACATACTTTCTAGTCATTATGAGAGCTACAATTTACACATTGCCGAGTTAACCAATGAAAATGGTCAGGTGGTTTGGAAAGCCTCATATGTTACCATAATGATTTTCATGTGGTTAACCTTG‑CTTTCGGTTAATCTATACTTTAATGGTTTACGCt              <  2:370366/251‑1 (MQ=255)                                                                                                                                                                                     aaaCACAAAACAGCGCAATTTATTTTCCAAAAAAATGACTTTGTGCATAAGTTTGTTTTT‑GTTTTTGGGGGGCCGATAGTTGCTCACATACTTTCTAGTCATTATGAGAGCTACAATTTACACATTGCCGAGTTAACCAATGAAAATGGTCAGGTGGTTTGGAAAGCCTCATATGTTACCATAATGATTTTCATGTGGTTAACCTTG‑CTTTCGGTTAATCTATACTTTAATTGTTTACGCTATGATATCTg    <  1:162730/251‑1 (MQ=255)                                                                                                                                                                                       acacAAAAAAGCGCAATTTATTTTCCAAAAAAATGACTTTGTGCATAAGTTTGTTTTT‑GTTTTTGGGGGGCCGATAGTTGCTCACATACTTTCTAGTCATTATGAGAGCTACAATTTACACATTGCCGAGTTAACCAATGAAAATGGTCAGGTGGTTTTGAAAGCCTCATATGTTACCATAATGATTTTCATGTGGTTAACCTTG‑CTTTCGGTTAATCTATACTTTAATGGTTTACGCTATGATATCTGGa  <  2:273422/251‑1 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                                                   TAGTTTGGCTTTTCTGAGTTGTTTGATTTACTTTGTTATAACCGTCATTATCATTCAGCAAATTATTGAGGAGCGTTTGACAAGCAGTGTTGTCCAAAATGACATTGCAATCTATTATTTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCAAAAAAATGACTTTGTGCATAAGTTTGTTTTTTGTTTTTGGGGGGCCGATAGTTGCTCACATACTTTCTAGTCATTATGAGAGCTACAATTTACACATTGCCGAGTTAACCAATGAAAATGGTCAGGTGGTTTGGAAAGCCTCATATGTTACCATAATGATTTTCATGTGGTTAACCTTG‑CTTTCGGTTAATCTATACTTTAATGGTTTACGCTATGATATCTGGA  >  NC_000913/1093106‑1093539

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑