Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 480,295 2 bp→AA coding (13‑14/219 nt) hha ← hemolysin expression‑modulating protein Hha

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913480,2950GA100.0% 108.9 / NA 33P5F (CCT→TTT) hhahemolysin expression‑modulating protein Hha
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (13/20);  total (13/20)
*NC_000913480,2960GA100.0% 106.1 / NA 33P5F (CCT→TTT) hhahemolysin expression‑modulating protein Hha
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (13/20);  total (13/20)

CGGTTATAGCTCCGAAAGCGAATTATTTATTAGCGAATAAATTTCCATACTG‑AGGAAGGGATCTTGTCGTACAGTTTATTCATGGTCAATTCGGCGAGGCGGTG‑ATCTGCGGCTGAGTAAAATACCGCCAGTTCATTATCTGATAATTCGTATTTATTTTTCTCGATAACACGCTCCAGCGTGTCAATTGTCTGGCAACGACGTAAACGCATTAAATAATCGGTTTTCGTTAAAGGTTTTTCGGACATACTTCTACCTATGGTTGTAATAATTCTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAGATCCTGCATATTAATACCATAACTACTGAACAACATAAAGGTGTCATCCAGATATTCGTCGATCTGCTCAATGAGCTTATTGTCTTCATTATACTTAATTT  >  NC_000913/480061‑480521
                                                                                                                                                                                                                                            ||                                                                                                                                                                                                                                 
gggTTACAGCGCCGAAAGCGAATTATTTATTAGTGAATAAATTTCCAT‑CTGGAGGAAGGGATCTTTTCGTACAGTTTATTCATTGACAAGTCGGCGAGG‑GGTGGATCTGCGGCTGAGTAAAAAGCCGCCAGTTCATTATCTGATAATTCGTATTTAGTTTTCTCAATAACACGCTCCAGCGTGTCAATTGTCTGGCAACGGCGTAAACGCATTAAATAATCGGTGTTCGTTATAAATTTTTCGGACATAcg                                                                                                                                                                                                                    <  2:243297/250‑2 (MQ=255)
 ggTTATAGCTCCGAAAGCGAATTATTTATTAGCGAATAAATTTCCATACTG‑CGGAAGGGATCTTGTCGTACAGTTTATTCATGGTCAATTCGGCGAGGCGGTG‑ATCTGCGGCTGAGTAAAATAACGCCAGTTCATTATCTGATTATTCGTATTTCTTTTTCTCGATAACACGCTCCAGCGTGTCAATTGTCTGGCACCGACGTAAACGCATTAAATAATCGGTTTTCGTTAAAAATTTTTCGGACATACtt                                                                                                                                                                                                                   >  1:192147/1‑251 (MQ=255)
       aGCTCCGACAGCGAATTATTTATTAGCGAATAAATTTCCATACTG‑AGGACGGGATCTTGTCGTACAGTTTATTCATGGTCAATTCGGCGAGGCGGTG‑ATCTGCGGCTGAGTAAAATACCGCCAGTTCATTATCTGATAATTCGTATTTATTTTTCTCGATAACACGCTCCAGCGTGTCAATTGTCTGGCAACGACGTAAACGCATTAAATAATCGGTTTTCGTTAAAAATTTTTCGGACATACTTCTACCt                                                                                                                                                                                                             >  1:196109/1‑251 (MQ=255)
       aGCTCCGAAAGCGAATTTTTTATTAGCGAAAAAATTTCCATACTG‑AGGAAGGGATCTTGTCGTACAGTTTATTCATGGTCAATTCGGCGAGGCGGTG‑ATCTGCGGCTGATTAAAATACCGCCAGTTCAGTATCTGATAATTCGTATTTATTTTTCTCGATAACACGCTCCAGCGTGTCAATTTTCTGGCAACGACGTAAACGCATTAAATAATCGGTTTTCGTTAAAAATTTTTCGGACATACTCCTACCt                                                                                                                                                                                                             <  2:323867/251‑1 (MQ=255)
         cgccGAAAGCGAATTATTTATTAGCGAATAAATTGCCATACGG‑AGGAAGGGATCTTGTCGTACAGTTTATTCATGGTCAATGCGGCGAGGCGGTG‑AGCTGCGGCTGAGTAAAATACCGCCAGTTCATTATCTGATAATTCGTATTTATTTTTCTCGATAACACGCTCCAGCGTGTCAATTGTCTGGCAACGACGTAAACGCATTAAATAATCGGTTTTCGTTAAAAATTTTTCGGACATACTTCTACCTAt                                                                                                                                                                                                           <  1:77360/249‑1 (MQ=255)
           ccGAAAGCGAATTATTTATTAGCGAATAAATTTCCATACTG‑AGGAAGGGATCTTGTCGTACAGTTTATTCATGGTCAATTCGGCGAGGCGGTG‑ATCTGCGGCTGAGTAAAATACCGCCAGTTCATTATCTGATAATTCGTATTTATTTTTCTCGATAACACGCTCCAGCGTGTCAATTGTCTGGCAACGACGTAAACGCATTAAATAATCGGTTTTCGTTAAAAATTTTTCGGACATACTTCTACCTAt                                                                                                                                                                                                           <  1:580417/249‑1 (MQ=255)
              aaaGCGAATTATTTATTAGCGAATAAATTGCCATACTG‑AGGAAGGGATCTTGTCGTACAGTTTATTCATGGTCAATTCGGCGAGGCGGTG‑ATCTGCGGCTGAGTAAAATACCGCCAGTTCATTATCTGATAATTCGTATTTATTTTTCTCGATAACACGCTCCAGCGTGTCAATTGTCTGGCAACGACGTAAACGCATTAAATAATCGGTTTTCGTTAAAAATTTTTCGGACATACTTCTACCTATGGTTg                                                                                                                                                                                                      <  2:175316/251‑1 (MQ=255)
                       tatttattAGCGAATAAATTTCCATACTG‑AGGAAGGGATCTTGTCGTACAGTTTATTCATGGTCAATTCGGCGAGGCGGTG‑ATCTGCGGCTGAGTAAAATACCGCCAGTTCATTATCTGATAATTCGTATTTATTTTTCTCGATAACACGCTCCAGCGTGTCAATTGTCTGGCCACGACGTAAACGCATTAAATAATCGGTTTTCGTTAAAAATTTTTCGGACATACTTCTACCTATGGTTTTAATAATTc                                                                                                                                                                                             >  2:101234/1‑251 (MQ=255)
                                      aaaTTTCCATACTG‑AGGAAGGGATCTTGTCGTACAGTTTATTCATGGACAATTCGGCGAGGCGGTG‑ATCTGCGGCTGAGTAAAATACCGCCAGTTCATTATCTGATAATTCGTATTTATTTTTCTCGATAACACGATCCAGCGTGTCAATTGTATGGCAACGACGTAAACGCATTAAATAATCGGTTTTCGTTAAAAATTTTTCGGACATACTTCTACCTATGGTTGTAATAATTCTTACAAGATAAACTc                                                                                                                                                                              >  1:35796/1‑251 (MQ=255)
                                             cATACTG‑AGGAAGGGATCTTGTCGTACAGTTTATTCATGGTCAATTCGGCGAGGCGGTG‑ATCTGCGGCTGAGTAAAATACCGCCAGTTCATTATCTGATAATTCGTATTTATTTTTCTCGATAACACGCTCCAGCGTGTCAATTGTCTGGCAACGACGTAAACGCATTAAATAATCGGTTTTCGTTAAAAATTTTTCGGACATACTTCTACCTATGGTTGTAATAATTCTAACAAGATAAACTCGCAGGAt                                                                                                                                                                       >  1:211819/1‑251 (MQ=255)
                                                   g‑AGGAAGGGATCTTGTCGTACAGTTTATCCTGGGTCAATTGGGCTAGGCGGTT‑ATCTGCGGCTGAGTAAAATACCGCCAGTTCATTATCTGATAATTCGTATTTATTTTTCTCGATAACACGCTCCAGCGTGTCAATGGTCTGGCAACGACGTAAACGCATTAAATAATCGGTTTTCGTTAAAAATTTTTCGGACATACTTCTACCTATGGTTGTAATAATTCTAACAAGAGAAACTCGCAGGATTCTCtt                                                                                                                                                                 <  2:284694/251‑1 (MQ=255)
                                                        aaGGGATCTTGTCGTACAGTTTATTCATGTTCAATTCGGCGAGGCGGTG‑ATCTGCGGCTGAGTAAAATACCGCCAGTTCATTATCTGATAATTCGTATTTATTTTTCTCGATAACACGCTCCAGCGTGTCAATTGTCTGGCAACGACGTAAACGCATTAAATAATCGGTTTTCGTTAAAAATTTTTCGGACATACTTCTACCTATGGTTGTAATAATTCTAACAAGATAAACTCGCAGGATTCTCTTtcgt                                                                                                                                                             <  1:121606/251‑1 (MQ=255)
                                                                 tGTCGTACAGTTTATTCATGGTCAATTCGGCGAGGCGGTG‑ATCTGCGGCTGAGTAAAATACCGCCAGTTCATTATCTGATAATTCGTATTTATTTTTCTCGATAACACGCTCCAGCGTGTCAATTGTCTGGCAACGACGTAAACGCATTAAATAATCGGTTTTCGTTAAAAATTTTTCGGACATACTTCTACCTATGGTTGTAATAATTCTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCAtt                                                                                                                                                       <  1:321503/248‑1 (MQ=255)
                                                                 tGTCGTACAGTTTATTCATGGTCAATTCGGCGAGGCGGTG‑ATCTGCGGCTGAGTAAAATACCGCCAGTTCATTATCTGATAATTCGTATTTATTTTTCTCGATAACACGCTCCAGCGTGTCAATTGTCTGGCAACGACGTAAACGCATTAAATAATCGGTTGTCGTTAAAAATTTTTCGGACATACTTCTACCTATGGTTGTAATAATTCTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCAt                                                                                                                                                        <  1:417553/247‑1 (MQ=255)
                                                                                              gCGAGGCGGTG‑ATCTGCGGCTGAGTAAAATACCGCCAGTTCATTATCTGATAATTCGTATTTATTTTTCTCGATAACACGCTCCAGCGTGTCAATTGTCTGGCAACGACGTAAACGCATTAAATAATCGGTTTTCGTTAAAAATTTTTCGGACATACTTCTACCTATGGTTGTAATAATTCTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACt                                                                                                                       >  1:364612/1‑251 (MQ=255)
                                                                                                gAGGCGGTG‑ATCTGCGGCTGGGTAAAATACCGCCAGTTCATTATCTGATAATTCGTATTTATTTTTCTCGATAACACGCTCCAGCGTGTCAATTGTCTGGCAACGACGTAAACGCATTAAATAATCGGTTTTCGTTAAAAATTTTTCGGACATACTTCTACCTATGGTTGTAATAATTCTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTc                                                                                                                     >  1:18538/1‑251 (MQ=255)
                                                                                                         gaacTGCGGCTGAGAAAAATACCGCCAGTTCATTATCTGATAATGCGTATTTATTTTTCTCGATAACACGCTCCAGCGTGTCAATTGTCTGGCAACGACGTAAACGCATTAAATAATCGGTTTTCGTTAAAAATTTTTCGGACATACTTCTACCTATGGTTGTAATAATTCTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCAttt                                                                                                             <  1:388214/248‑1 (MQ=255)
                                                                                                                    gAGTAAAATACCGCCAGTTCATTATCTGATAATTCGTATTTATTTTTCTCGATAACACGCTCCAGCGTGTCAATTGTCTGGCAACGACGTAAACGCATTAAATAATCGGTTTTCGTTAAAAATTTTTCGGACATACTTCTACCTATGGTTGTAATAATTCTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGGCGATTCCCTGACTTCCGCCATTTCTGAAGATcc                                                                                                   <  2:234434/250‑1 (MQ=255)
                                                                                                                            tACCGCCAGTTCATTATCTGATAATTCGTATTTATTTTTCTCGATAACACGCTCAAGCGTGTCAATTGTCTGGCAACGACGTAAACGCATTAACTAATCGGTTTTCGTTAAAAATTTTTCGGACATACTTCTACCTATGGTTGTAATAATTCTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAGATCCTGCATAtt                                                                                           >  2:87271/1‑250 (MQ=255)
                                                                                                                                   aGTTCATTATCTGATAATTCGTATTTATTTTTCTCGATAACACGCTCCAGCGTGTCAATTGTCTGGCAACGACGTAAACGCATTAAATAATCGGTTTTCGTTAAAAATTTTTCGGACATACTTCTACCTATGGTTGTAATAATTCTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAGATCCTGCATATTAATACCAt                                                                                   <  1:13379/251‑1 (MQ=255)
                                                                                                                                         ttATCTGATAATTCGTATTTGTTTTTCTCGATAACACGCTCCATCGTGTCAATTGTCTGGCAACGACGTAAACGCATTAAATAATCGGTTTTCGTTAAAAATTTTTCGGACATACTTCTACCTATGGTTGTAATAATTCTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAGATCCTGCATATTAATACCATAactac                                                                             >  2:398772/1‑251 (MQ=255)
                                                                                                                                                     tCGTATTTATTTTTCTCGATAACACGCTCCAGCGTGTCAATTGTCTGGCAACGACGTAAACGCATTAAATAATCGGTTTTCGTTAAAAATTTTTCGGACATACTTCTACCTATGGTTGTAATAATTCTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAGATCCTGCATATTAATACCATAACTACTGAACAACATa                                                                  <  1:228634/250‑1 (MQ=255)
                                                                                                                                                     tCGTATTTATTTTTCTCGATAACACGATCCAGCGTGTCAATTGTCTGTCAACGACGTAAACGCATTAAATAATCGGTTTTCGTTAAAAATTTTTCGGACATACTTCTACCTATGGTTGTAATAATTCTAACAATATAAACTCGCAGGATTCTCTTGCGTCGCATTGACAAAACATCGGAATATTCGATTGCCTGACTTCCGCCATTTCTGAAGATCCTGCATATTAAGACCATAACTACTGAACAACATa                                                                  <  2:58705/250‑1 (MQ=255)
                                                                                                                                                                  tctcGATAACACGCTCCAGCGTGTCAATTGTCTGGCAACGACGTAAACGCCTTAAATAATCGGTTTTCGTTAAAAATTTTTCGGACATACTTCTACCTATGCTTGTAATACTTCTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCAGCCATTTCTGAAGATCCTGCATATTAATACCATAACTACTGAACAACATAAAGGTGTCATcc                                                      >  1:108722/1‑249 (MQ=255)
                                                                                                                                                                     cGATAACACGCTCCAGCGTGTCAATTGTCTGGCAACGACGTAAACGCATTAAATAATCGGTTTTCGTTAAAAATTTTTCGGACATACTTCTACCTATGGTTGTAATAATTCTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAGATCCTGCATATTAATACCATAACTACTGAACAACATAAAGGTGTCATCCAg                                                    <  1:418332/248‑1 (MQ=255)
                                                                                                                                                                                tCCAGCGTGTCAATTGTCTGGCAACGACGTAAACGCATTAAATAATCGGTTTTCGTTAAAAATTTTTCGGACATACTTCTACCTATGGTTGTAATAATTCTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAGATCCTGCATATTAATACCATAACTACTGAACAACATAAAGGAGTCATCCAGATATTCGTCGATCt                                      >  2:319109/1‑251 (MQ=255)
                                                                                                                                                                                 ccAGCGTGTCAATTGTCTGGCAACGACGTAAACGCATTAAATAATCGGTTTTCGTTAAAAATTTTTCGGACATACTTCTACCTATGGTTGTAATAATTCTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAGATCCTGCATATTAATACCATAACTACTGAACAACATAAAGGTGTCATCCAGATATTCGTCGATCt                                      <  1:60349/250‑1 (MQ=255)
                                                                                                                                                                                 ccAGCGTGTCAATTGTCTGGCAACGACGTAAACGCATTAAATAATCGGTTTTCGTTAAAAATTTTTCGGACATACTTCTACCTATGGTTGTAATAATTCTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAGATCCTGCATATTAATACCATAACTACTGAACAACATAAAGGTGTCATCCAGATATTCGTCGATCt                                      <  1:481505/250‑1 (MQ=255)
                                                                                                                                                                                                   tgCAACTACGAAAACGCATTAAATAATCGGTTTACGTTAAAAATTTTTCGGACATTCATCTTCCTATGGTTGTAATAATTCTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAGATCCTGCATATTAATACCATAACTACTGAACAACATAAAGGTGTCATCCAGATATTCGTCGATCTGTTCAATGAGCTTATTGt                    <  2:423199/249‑1 (MQ=255)
                                                                                                                                                                                                   ggCAACGACGTAAACGCATTAAATAATCGGTTTTCGTTAAAAATTTTTCGGACATACTTCTACCTATGGTTGTAATAATTCTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAGATCCTGCATATTAATACCATAACTACTGAACAACATAAAGGTGTCATCCAGATATTCGTCGATCTGCTCAATGAGCTTATTg                     <  1:185755/249‑1 (MQ=255)
                                                                                                                                                                                                   ggCAACGACGTAAACGCATTAAATAATCGGTTTTCGTTAAAAATTTTTCGGACATACTTCTACCTATGGTTGTAATAATTCTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAGATCCTGCATATTAATACCATAACTACTGAACAACATAAAGGTGTCATCCAGATATTCGTCGATCTGCTCAATGAGCTTATTGTc                   <  1:87890/251‑1 (MQ=255)
                                                                                                                                                                                                                   cATTAAATAATCGGTTTTCGTTAAAAATTTTTCGGACATACTTCTACCTATGGTTGTAATAATTCTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAGATCCTGCATATTAATACCATAACTACTGAACAACATAAAGGTGTCATCCAGATATTCGTCGATCTGCTCAATGAGCTTATTGTCTTCATTATACTTAAtt   >  1:324425/1‑251 (MQ=255)
                                                                                                                                                                                                                    aTTAAATAATCGGTTTTCGTTAAAAATTTTTCGGACATACTTCTACCTATGGATGTAATAATTCTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAGATCCTGCATATTAATACCATAACTACTGAACAACATAAAGGTGTCATCCAGATATTCGTCGATCTGCTCAATGAGCTTATTGTCTTCATTATACTTAAttt  >  1:356358/1‑251 (MQ=255)
                                                                                                                                                                                                                                            ||                                                                                                                                                                                                                                 
CGGTTATAGCTCCGAAAGCGAATTATTTATTAGCGAATAAATTTCCATACTG‑AGGAAGGGATCTTGTCGTACAGTTTATTCATGGTCAATTCGGCGAGGCGGTG‑ATCTGCGGCTGAGTAAAATACCGCCAGTTCATTATCTGATAATTCGTATTTATTTTTCTCGATAACACGCTCCAGCGTGTCAATTGTCTGGCAACGACGTAAACGCATTAAATAATCGGTTTTCGTTAAAGGTTTTTCGGACATACTTCTACCTATGGTTGTAATAATTCTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAGATCCTGCATATTAATACCATAACTACTGAACAACATAAAGGTGTCATCCAGATATTCGTCGATCTGCTCAATGAGCTTATTGTCTTCATTATACTTAATTT  >  NC_000913/480061‑480521

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑