Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,853,977	C→T	M1M (ATG→ATA) †	ydjE ←	putative transporter YdjE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,853,977	0	C	T	100.0%	120.4 / NA	36	M1M (ATG→ATA) †	ydjE	putative transporter YdjE
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (17/19);  total (17/19)

CACCAATAAACCCACCAGTAAGTGAGCCGATGAAATAACCAAACATTAATGCCGAGGTAAAGGCGGCATTGAGGAAATTATTTGACCATCCATTGCTTACCAGCTTTGCTAAGACGACATTACCGGAGTAACTCAAAAACCCTGTTAATAACAGACTAAAGCTTATAATACCAAATATACGATAATGAAACCGGGCCAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCCATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCAAGGCGCAGGAACAAATAATATTGACAACGTGTGCTTAAATTACCACACCAGCTGGATATTATTGTCTTTGCAAAATGCTATCCACTCTGCACCCGGTGATTTATCAGTTATTATGTAATTAATATGACTAAAATCAGCTAACTGGACAAAAGCTTTGCG  >  NC_000913/1853736‑1854215                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                               cACCAATAAACCCACCAGTAAGTGAGCCGATGAAATAACCAAACATTAATGCCGAGGTAAAGGCGGCATTGAGGAAATTATTTGACCATCCATTGCTTACCAGCTTTGCTAAGACGACATTACCGGAGTAACTCAAAAACCCTGTTAATAACAGACTAAAGCTTATAATACCAAATATACGATAATGAAACCGGGCCAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCTATTAGGTGc                                                                                                                                                                                                                                       >  1:296558/1‑251 (MQ=255) cACCAATAAACCCACCAGTAAGTGAGCCGATGAAATAACCAAACATTAATGCCGAGGTAAAGGCGGCATTGAGGAAATTATTTGACCATCCATTGCTTACCAGCTTTGCTAAGACGACATTACCGGAGTAACTCAAAAACCCTGTAAATAACAGACTAAAGCTTATAATACCAAATATACGATAATGAAACCGGGCCAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCTATTAGGTg                                                                                                                                                                                                                                        <  2:125680/250‑1 (MQ=255)   ccAATAAACCCACCAGTAAGTGAGCCGATGAAATAACCAAACATTAATGCCGAGGTAAAGGCGGCATTGAGGAAATTATTTGACCATCCATTGCTTACCAGCTTTGCTAAGACGACATTACCGGAGTAACTCAAAAACCCTGTTAATAACAGACTAAAGCTTATAATACCAAATATACGATAATGAAACCGGGCCAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCTATTAGGTGCCt                                                                                                                                                                                                                                     <  2:296558/251‑1 (MQ=255)    cAATAAACCCACCAGTAAGTGAGCCGATGAAATAACCAAACATTAATGCCGAGGTAAAGGCGGCATTGAGGAAATTATTTGACCATCCATTGCTTACCAGCTTTGCTAAGACGACATTACCGGAGTAACTCAAAAACCCTGTTAATAACAGACTAAAGCTTATAATACCAAATATACGATAATGAAACCGGGCCAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCTATTAGGTGcccc                                                                                                                                                                                                                                    <  1:299420/251‑3 (MQ=255)    cAATAAACCCACCAGTAAGTGAGCCGATGAAATAACCAAACATTAATGCCGAGGTAAAGGCGGCATTGAGGAAATTATTTGACCATCCATTGCTTACCAGCTTTGCTAAGACGACATTACCGGAGTAACTCAAAAACCCTGTTAATAACAGACTAAAGCTTATAATACCAAATATACGATAATGAAACCGGGCCAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCTATTAGGTGCCTc                                                                                                                                                                                                                                    <  1:299398/251‑1 (MQ=255)    cAATAAACCCACAAGTAAGTGAGCCGATGAAATAACCAAACATTAATGACGAGGTAAAGGCGGCATTGAGGAAATTAGTTGACCATCCAGTGCTTACCAGCTTTGCTAAGACGACATTACCGGAGTAACGCAAAAACCCTGTTAATAACAGACTAAAGCTTATAATACCAAATATACGATAATGAAACCGGTCCAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCTATTAGGTGCCTc                                                                                                                                                                                                                                    <  1:96294/251‑1 (MQ=255)         aacacaacaGTAAGTGAGCAGATGAAATAACCACACATTAAGGACGAGAAAAAGACGGCATTGCGCAAAATATTTGAACATCCATTGCTTAACAGCTTTGCTAAGACGACATTACCGGAGTAACTCACAAAACCTGTTAATAACAGCCTAAAGATTATAATACCAAATATACGATAATGAAACCGGGCCAAAGGCAAACGGTCAAGTATTGCGCCAATTAGATCATATTGTTCTATAAGGCGCCTCGGagt                                                                                                                                                                                                                               >  2:519980/8‑249 (MQ=255)                caGTAAGTGAGCCGATGAAATAACCAAACATTAATGCCGAGATAAAGGCAGCATTGAGGAAATTATTTGACCCCCCATTGCTTACCAGCTTTGCTAAGACGAAATTACCGGAGTAACTCAAAAACCCTGTTAATAACAGAATAACGCTTATAATACCAAATATACGATAATGAAACCGGGCCAAAGGCAAGCGGACCAGTCTTGCACCAATTTGATCAAATAGTTCTAATCGGCGCCTCGGATTTGCTac                                                                                                                                                                                                                         >  1:552525/1‑249 (MQ=255)                  gTAAGTGAGCCGATGAAATAACCAAACATTAATGCCGAGGTAAAGGCGGCATTGAGGAAATTATTTGACCATCCATTGCTTACCAGCTTTGCTAAGACGACATTACCGGAGTAACTCAAAAACCCGGTTAATAACAGACTAAAGCTTATAATACCAAATATACGATAATGAAACCGGGCCAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCTAGTAGGTGCCTCGGATTTGCTaaaaa                                                                                                                                                                                                                      <  2:418065/251‑1 (MQ=255)                     aGTGAGCCGATGAAATAACCAAACATTAATGCCGAGGTCAAGGCGGCATTGAGGAAATTATTTGACCATCCATTGCTTACCAGCTTTGCTAAGACGACATTACCGGAGTAACTCAAAAACCCTGTTAATAACAGACTAAAGCTTATAATACCAAATATACGATAATGAAACCGGGCCAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCTATTAGGTGCCTCGGATTTGCTAAAAAgg                                                                                                                                                                                                                    >  2:255000/1‑250 (MQ=255)                      gTGAGCCGATGAAATAACCAAACATTAATGCCGAGGTAAAGGCGGCATTGAGGAAATTATTTGACCATCCATTGCTTACCAGCTTTGCTAAGACGACATTACCGGAGTAACTCAAAAACCCTGTTAATAACAGACTAAAGCTTATAATACCAAATATACGATAATGAAACCGGGCCAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCTATTAGGTGCCTCGGATTTGCTAAAAAGGTa                                                                                                                                                                                                                  <  2:289386/251‑1 (MQ=255)                              aTGAAATAACCACACATTAATGCCGAGGTAAAGGCGGCATTGAGGAAATTCTTTGACCATCCATTGCTTACCAGCTTTGCTAAGACGACATTACCGGAGTAACTCAAAAACCCTGTTAATAACAGACTAAAGCTTATAATACCAAATATACGATAATGAAACCGGGCCAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCTATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGc                                                                                                                                                                                                          >  1:217623/1‑251 (MQ=255)                                                         gTAAAGGCGGCATTGAGGAAATTATTTGACCATCCATTGCTTACCAGCTTTGCTAAGACGACATTACCGGAGTAACTCAAAAACCCTGTTAATAACAGACTAAAGCTTATAATACCAAATATACGATAATGAAACCGGGCCAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCTATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTatc                                                                                                                                                                               >  1:480306/1‑251 (MQ=255)                                                                       gAGGAAATTATTTGACCATCCATTGCTTACCAGCTTTGCTAAGACGACATTACCGGAGTAACTCAAAAACCCTGTTAAGAACAGACTAAAGCTTATAATACCAAATATACTATAATGAAACCGGGCCAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCTATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTc                                                                                                                                                                  <  2:117589/250‑1 (MQ=255)                                                                                       catccatTGCTTACCAGCTTTGCTAAGACGACATTACCGGAGTAACTCAAAAACCCTGTTAATAACAGACTAAAGCTTATAATACCAAATATACGATAATGAAACCGGGCCAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCTATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCAAGGCGCAGGAACAaat                                                                                                                                                 <  1:62903/251‑1 (MQ=255)                                                                                          ccatTGCTTACCAGCTTTGCTAAGACGTCATTACCGGAGTAACTCAAAAACCCTGTTAATAACAGACTAAAGCTTATAATACCAAATATACGATAATGAAACCGGGACAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCTATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACATGGCGCATCTTTATCATCAGTTTATTTCAAGGCGCAGGAACAaaaaat                                                                                                                                              <  2:112900/251‑1 (MQ=255)                                                                                             ttGCTTACCAGCTTTGCTAAGACGACATTCCCGGAGTAACTCAAACACCCTGTTAATAACAGACTCAAGCTTATAATACCAAATATACGATACTGAAACCGGGCCAAAGGCCAGCGGTCCAATCTTGCGCCAATTTGATCATATTGTTCTATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCACTTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCAAGGCGCAGGAACAAATAATAtt                                                                                                                                           >  2:199030/1‑251 (MQ=255)                                                                                                                        ttACCGGAGTAACTCAAAAACCCTGTTAATAACAGACTAAAGCTTATAATACCAAATATACGATAATGAAACCGGGCCAAAGGCAAGCGGTCCAGTCTTGCGACAATTTGATCATATTGTTCTATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCAAGGCGCAGGACCAAATAATATTGACAACGTGTGCTTAAATTACCACAcc                                                                                                                >  2:187989/1‑251 (MQ=255)                                                                                                                                          aaCCCTGTTAATAACAGACTAAAGCTTATAATACCAAATATACGATAATGAAACCGGGCCAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCTATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCAAGGCGCAGGAACAAATAATATTGACAACGTGTGCTTAAATTACCACACCAGCTGGATATTATTGTCt                                                                                              <  1:295910/251‑1 (MQ=255)                                                                                                                                                   aataaCAGACTAAAGCTTATAATACCAAATATACGATAATGAAACCGGGCCAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCTATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCAAGGCGCAGGAACAAATAATATTGACAACGTGTGCTTAAATTACCACACCAGCTGGATATTATTGTCTTTGCAAAAt                                                                                     <  1:224750/251‑1 (MQ=255)                                                                                                                                                     aaaCAGACTAAAGCGTATAATGCCAAATATACGATAATGAAACCGGGCCAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCTATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGTCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCAAGGCGCAGGAACAAATAATATTGACAACGTGTGCTTAAATTACCACACCAGCTGGATATTATTGTCTTTGCAAAATGc                                                                                   <  1:273097/250‑1 (MQ=255)                                                                                                                                                      aaCAGACTAAAGCTTATAATACCAAATATACGATAATGCAACCGGGCCAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCTATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCAAGGCGCAGGAACAAATAATATTGACAACGTGTGCTTAAATTACCACACCAGCTGGATATTATTGTCTTTGCAAAATGCt                                                                                  >  2:470651/1‑251 (MQ=255)                                                                                                                                                         aGACTAAAGCGTATAATACCAAATATACGATAATGAAACCGGGCCAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCTATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCAAGGCGCAGGAACAAATAATATTGACAACGTGTGCTTAAATTACCACACCAGCTGGATATTATTGTCTTTGCAAAATGCTATc                                                                               <  2:570665/251‑1 (MQ=255)                                                                                                                                                                       aataCCAAATATACGATAATGAAACCGGGCCAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCTATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCAAGGCGCAGGAACAAATAATATTGACAACGTGTGCTTAAATTACCACACCAGCTGGATATTATTGTCTTTGCAAAATGCTATCCGCTCTGCACCCg                                                                  <  1:187989/250‑1 (MQ=255)                                                                                                                                                                           ccAAATATACGATAATGAAACCGGGCCAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCTATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCAAGGCGCAGGAACAAATAATATTGACAACGTGTGCTTAAATTACCACACCAGCTGGATATTATTGTCTTTGCAAAATGCTATCCACTCTGCACCCGGTGAt                                                             >  1:392306/1‑251 (MQ=255)                                                                                                                                                                                        aaTGAAACCGGGCCAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCTATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCAAGGCGCAGGAACAAATAATATTGACAACGTGTGCTTAAATTACCACACCAGCTGGATATTATTGTCTTTGCAAAATGCTATCCACTCTGCACCCGGTGATTTATCAGttatta                                                <  1:190366/251‑1 (MQ=255)                                                                                                                                                                                        aaTGAAACCGGGCCAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCTATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCAAGGCGCAGGAACAAATAATATTGACAACGTGTGCTTAAATTACCACACCAGCTGGATATTATTGTCTTTGCAAAATGCTATCCACTCTGCACCCGGTGATTTATCAGttatt                                                 <  1:120063/250‑1 (MQ=255)                                                                                                                                                                                          tGAAACCGGGCCAAAGGCACGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCTATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTatcat                                                                                                                                                                             >  1:164506/1‑124 (MQ=255)                                                                                                                                                                                          tGAAACCGGGCCAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCTATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTatcat                                                                                                                                                                             <  2:164506/124‑1 (MQ=255)                                                                                                                                                                                                       aaGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCTATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCAAGGCGCAGGAACAAATAATATTGACAACGTGTGCTTAAATTACCACACCAGCTGGATATTATTGTCTTTGCAAAATGCTATCCACTCTGCACCCGGTGATTTATCAGTTATTATGTAATTAATATGAc                                 >  1:107730/1‑251 (MQ=255)                                                                                                                                                                                                            aaGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCTATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCAAGGCGCAGGAACAAATAATATTGACAACGTGTGCTTAAATTACCACACCAGCTGGATATTATTGTCTTTGCAAAATGCTATCAACTCTGCACCCGGTGATTTATCAGTTATTATGTAATTAATATGACTaaa                             >  1:464558/1‑250 (MQ=255)                                                                                                                                                                                                                   ccAGTCTTGCGCCAATTTGATCATATTGTTCTATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCAAGGCGCAGGAACAAATAATATTGACAACGTGTGCTTAAATTACCACACCAGCTGGATATTATTGTCTTTGCAAAATGCTATCCACTCTGCACCCGGTGATTTATCAGTTATTATGTAATTAATATGACTAAAATCAGCTa                     <  2:333374/251‑1 (MQ=255)                                                                                                                                                                                                                        cTTGCGCCAATTTGATCATATTGTTCTATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCAAGGCGCAGGAACAAATAATATTGACAACGTGTGCTTAAATTACCACACCAGCTGGATATTATTGTCTTTGCAAAATGCTATCCACTCTGCACCCGGTGATTTATCAGTTATTATGTAATTAATATGACTAAAATCAGCTAACTgg                >  1:574150/1‑251 (MQ=255)                                                                                                                                                                                                                                 aTTTGATCATATTGTTCTATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCAAGGCGCAGGAACAAATAATATTGACAACGTGTGCTTAAATTACCACACCAGCTGGATATTATTGTCTTTGCAAAATGCTATCCACTCTGCACCCGGTGATTTATCAGTTATTATGTAATTAATATGACTAAAATCAGCTAACTGGACAAAAGCt       >  2:444975/1‑251 (MQ=255)                                                                                                                                                                                                                                     gATCATATTGTTCTATTAGGTGCCTCGGATTTGCTCAAAAGGTAGCAATTGCGGCAAATTCACTTGCAGCATCTTTATCATCATTTTATTTCAAGGCGCAGGAACAAATAATATTGACAACGTGTGCTTAAATTACCACACCAGCTGGATATTATTGTCTTTGCAAAATGCTATCCACTCTGCACCCGGTGATTTATCAGTTATTATGTAATTAATATGACTAAAATCAGCTAACTGGACAAAAGCTTTGc   >  2:537838/1‑251 (MQ=255)                                                                                                                                                                                                                                      aTCATATTGATCTATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCAAGGCGCAGGAACAAATAATATTGACAACGTGTGCTTAAATTACCACACCAGCTGGATATTATTGTCTTTGCAAAATGCTATCCACTCTGCACCCGGAGATTTATCAGTTATTATGTAATTAATATGACTAAAATCAGCTAACTGGACAAAAGCTTTgtg  >  2:24029/1‑249 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                               CACCAATAAACCCACCAGTAAGTGAGCCGATGAAATAACCAAACATTAATGCCGAGGTAAAGGCGGCATTGAGGAAATTATTTGACCATCCATTGCTTACCAGCTTTGCTAAGACGACATTACCGGAGTAACTCAAAAACCCTGTTAATAACAGACTAAAGCTTATAATACCAAATATACGATAATGAAACCGGGCCAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCCATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCAAGGCGCAGGAACAAATAATATTGACAACGTGTGCTTAAATTACCACACCAGCTGGATATTATTGTCTTTGCAAAATGCTATCCACTCTGCACCCGGTGATTTATCAGTTATTATGTAATTAATATGACTAAAATCAGCTAACTGGACAAAAGCTTTGCG  >  NC_000913/1853736‑1854215

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑