Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,844,146	C→T	A242A (GCG→GCA)	ynjI ←	DUF1266 domain‑containing protein YnjI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,844,146	0	C	T	100.0%	84.3 / NA	25	A242A (GCG→GCA)	ynjI	DUF1266 domain‑containing protein YnjI
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (12/13);  total (12/13)

AAACTGCTTGTCGTAACGATAACACGCTTCAAGTTCAGCATCCGTTAACTTTCTGCGATAGCAGCAGATATGCCAGTAAAGAAATCCCATTTGACTATTTTTTTGATAATCTTCTTCGCTTTCGAACAACTCGTGCGCCTTTCGAGAAGCAAGCATTATATAATGCCAGGCCAGTTCTTCTTCAATTGTCCCGTTTTGAAAAGCTGTGCTTGATATCGAGATCATCCATGATAATTCCGCCGCCCATATTAGCTTCGCCGAGGATTTACCGGAGCTATGATTAGCGCAATCAGAGATATAGTCTGAGGGAAAAACAGCAAATTTATTCAACAAGGCGATAACCTGCTCTGGGGCTTCCTCCATGTTTGCTTTAAAGGTATTGGCTCCATGGTCGCCAGAAAGAAAATGCTCCATTAAGGCACAATAACTTTCGCTATCTTCGATACCCCATTGATCCTCTAAAGACTCGCGTCTTTTACTT  >  NC_000913/1843909‑1844389                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                    aaaCTGCTTGTCGTAACGATAACACGCTTCAAGTTCAGCATCCGTTAACTTTCTGCGATAGCAGCAGATATGCCAGTAAAGAAATCCCATTTGACTATTTTTTTGATAATCTTCTTCGCTTTCGAACAACTCGTGCGCCTTTCGAGAAGCAAGCATTATCTAATGCCAGGCCAGTTCTTCTTCAATTGTCCCGTTTTGAAAAGCTGTGCTTGATATCGAGATCATCCATGATAATTCTGCCGCCCATAtt                                                                                                                                                                                                                                         >  1:482802/1‑250 (MQ=255)    cTGCTTATCGTAACGATAACACGCTTCCAGTTCAGCATCAGTTAACTTTCTGAGATAGCAGCAGATGTGCAAGTAAAGAAAACAAGTTTGACTATTTTTTTGATAATCTTCTTCGCTTTCGAACAACTCGTGCGCATTTCGAGAAGCAAGCATTATATAATGCCAGGCCAGTTCTTCTTCAATTGTCCCGTTTTGAAAAGCTGGGCTTGATATCGAGAACATCCATGCTAATTCTGCCGCACATATTAGCt                                                                                                                                                                                                                                     >  1:536556/1‑251 (MQ=255)       cTTGTCGTAACGATAACACGCTTCAAGTTCAGCATCCGTTAACTTTCTGCGATAGCAGCCGATATGCCAGTAAAGAAATCCCATTTGACTATTTTTTTGATAATCTTCTTCGCTTTCGAACAACTCATGCGCCTTTCGAGAAGCAAGCATTATATAATGCCAGGCCAGTTCTTCTTCAATTGTCCCGTTTTGAAAAGCTGTGCTTGATATCGAGATCATCCATGATAATTCTGCCGCCCATATTAGCTTCg                                                                                                                                                                                                                                  >  1:74016/1‑251 (MQ=255)                                  ttCAGCATCCGTTAACTTTCTGCGATAGCAGCAGCTATGCCAGTAAAGAAATCCCATTTGACTATTTTTTTGATAATCTTCTTCGCTTTCGAACAACTCGTGCGCCTTTCGAGAAGCAAGCATTATATAATGCCAGGCCAGTTCTTCTTCAATTGTCCCGTTTTGAAAAGCTGTGCTTGATATCGAGATCATCCATGATAATTCTGCCGCCCATATTAGCTTCGCCGAGGATTTACCGGAGCTATGAtt                                                                                                                                                                                                         >  1:497900/1‑249 (MQ=255)                                        aTCCGTTAACTTTCTGCGATAGCAGCAGATATGCCAGTAAAGAAATCCCATTTGACTATTTTTTTGATAATCTTCTTCGCTTTCGAACAACTCGTGCGCCTTTCGAGAAGCAAGCATTATATAATGCCAGGCCAGTTCTTCTTCAATTGTCCCGTTTTGAAAAGCTGTGCTTGATATCGAGATCATCCATGATAATTCTGCCGCCCATATTAGCTTCGCCGAGGATTTACCGGAGCTATGATTAGCGCAAt                                                                                                                                                                                                 >  2:162234/1‑251 (MQ=255)                                                        gcaatgcaggagaaaatgcAGTAAAGAAATCCCATTTGACTATTTTTTTGATAATCTTCTTCGCTTTCGAACAACTCGTGCGCCTTTCGAGAAGCAAGCATTATATAATGCCAGGCCAGTTCTTCTTCAATTGTCCCGTTTTGAAAAGCTGTGCTTGATATCGAGATCATCCATGATAATTCTGCCGCCCATATTAGCTTCGCCGAGGATTTACCGGAGCTATGATTAGCGCAATCAGAGATATAGTc                                                                                                                                                                                    <  1:377165/230‑1 (MQ=255)                                                          aTAGCAGCAGATATGCCAGTAAAGAAATCCCATTTGACTATTTTTTTGATAATCTTCTTCGCTTTCGAACAACTCGTGCGCCTTTCGAGAAGCAAGCATTATATAATGCCAGGCCAGTTCTTCTTCAATTGTCCCGTTTTGAAAAGCTGTGCTTGATATCGAGATCATCCATGATAATTCTGCCGCCCATATTAGCTTCGCCGAGGATTTACCGGAGCTATGATTAGCGCAATCAGAGATATAGTCTGAgg                                                                                                                                                                               <  1:282991/251‑1 (MQ=255)                                                               agcagATATGCCAGTAAAGAAATTCCATTTGACTATTTTTTTGATAATCTTCTTCGCTTTCGAACAACTCGTGCGCCTTTCGAGAAGCAAGCATTATATAAGGCCAGGCCAGTTCTTCTTCAATTGTCCCGTTTTTAAAAGCTTTGCTTGATATCGAGATCATCCATTATAATTCTGCCGCCCATAATAGCGTCGCCGAGGATTTACCGGAGCTATGATTATCGCAATCAGAGATAGAGTCTGAGGGaaaa                                                                                                                                                                          <  2:381008/251‑1 (MQ=255)                                                                              aaaGAAATCCCATTTGACTATTTTTTTGATAATCTTCTTCGCTTTCGAACAACTCGTGCGCCTTTCGAGAAGCAAGCATTATATAATGCCAGGCCAGTTCTTCTTCAATTGTCCCGTTTTGAAAAGCTGTGCTTGATATCGAGATCATCCATGATAATTCTGCCGCCCATATTAGCTTCGCCGAGGATTTACCGGAGCTATGATTAGCGCAATCAGAGATATAGTCTGAGGGAAAAACAGCAAATTTAtt                                                                                                                                                            <  1:46400/250‑1 (MQ=255)                                                                              aaaGAAACGCCATTTGACGATTTTTTTTATAATCTTCGCCGCTTGCGAACAACTCGTTCGCCTTTGGAGAAGCAAGCATTATTTAATGCCAGGCCAGTTCTTCTTCAATTGTCCCGTTTTGAAAAGTTGTGCTTGATATCGATATCATCCATGATAAGTCTGCCGCTCATATTAGCTTCGCCGAAGATTTACCGGAGGTATGATTAGCGCAATCAGAGATATAGTCTGAGGGAAAAACAGCAAATTTAtt                                                                                                                                                            <  1:38297/250‑1 (MQ=255)                                                                                           ttGACTATTTTTTTGATAATCTTCTTCGCTTTCGAACAACTCGTGCGCCTTTCGAGAAGCAAGCATTATATAATGCCAGGCCAGTTCTTCTTCAATTGTCCCGTTTTGAAAAGCTGTGCTTGATATCGAGATCATCCATGATAATTCTGCCGCCCATATTAGCTTCGCCGAGGATTTACCGGAGCTATGATTAGCGCAATCAGAGATATAGTCTGAGGGAAAAACAGCAAATTTATTCAACAAGGCGAt                                                                                                                                                <  2:169686/249‑1 (MQ=255)                                                                                           ttGACTATTTTTTTGATAATCTTCTTCGCTTTCGAACAACTCGTGCGCCTTTCGAGAAGCAAGCATTATATAATGCCAGGCCAGTTCTTCTTCAATTGTCCCGTTTTGAAAAGCTGTGCTTGATATCGAGATCATCCATGATAATTCTGCCGCCCATATTAGCTTCGCCGAGGATTTACCGGAGCTATGATTAGCGCAATCAGAGATATAGTCTGAGGGAAAAACAGCAAATTTATTCAACAAGGCGAt                                                                                                                                                >  1:169686/1‑249 (MQ=255)                                                                                                                    tcGCTTTCGAACAACTCGGGCGCCTTTCGAGAAGCAAGCATTATATAATGCCAGGCCAGTTCTTCTTCAATTGTCCCGTTTTGAAAAGCTGTGCTTGATATCGAGATCATCCATGATAATTCTGCCGCCCATATTAGCTTCGCCGAGGATTTACCGGAGCTATGATTAGCGCAATCAGAGATATAGTCTGAGGGAAAAACAGCAAATTTATTCAACAAGGCGATAACCTGCTCTGGGGCTTCCTCCATGt                                                                                                                      <  1:544958/250‑1 (MQ=255)                                                                                                                     cGCTTTCGAACAACTCGTGCGCCTTTCGATAAGCAAGCATTATATAATGCCAGGCCAGTTCTTCTTCAATTGTCCCGTTTTGAAAAGCTGTGCTTGATATCGAGATCATCCATGATAATTCTGCCGCCCATATTATCTTCGCCGAGGATTTACCTGAGCTATGATTAGCGCAATCAGAGATATAGCCTGAGGGAAAAACAGCAAATTTATTCAACAAGTCGATAACCTGctcttggccgttcaccatgttt                                                                                                                    <  2:356274/251‑19 (MQ=255)                                                                                                                           cGAACAACTCGTGCGCCTTTCGAGAAGCAAGCATTATATAATGCCAGGCCAGTTCTTCTTCAATTGTCCCGTTTTGAAAAGCTGTGCTTGATATCGAGATCATCCATGATAATTCTGCCGCCCATATTAGCTTCGCCGAGGATTTACCGGAGCTATGATTAGCGCAATCAGAGATATAGTCTGAGGGAAAAACAGCAAATTTATTCAACAAGGCGATAACCTGCTCTGGGGCTTCCTCAATGTTTGCttt                                                                                                               >  2:163672/1‑250 (MQ=255)                                                                                                                                               cGAGAAGCAAGCATTATATAATGCCAGGCCAGTTCTTCTTCAATTGTCCCGTTTTGAAAAGCTGTGCTTGATATCGAGATCATCCATGATAATTCTGCCGCCCATATTAGCTTCGCCGAGGATTTACCGGAGCTATGATTAGCGCAATCATAGATATAGTCTGAGGGAAAAACAGCAAATTTATTCAACAAGGCGATAACCTGCTCTGGGGCTTCCTCCATGTTTGCTTTAAAGGTATTGGCTCCATGGt                                                                                           <  1:386845/250‑1 (MQ=255)                                                                                                                                               cGAGAAGCAAGCATTATATAATGCCAGGCCAGTTCTTCTTCAATTGTCCCGTTTTGAAAAGCTGTGCTTGATATCGAGATCATCCATGATAATTCTGCCGCCCATATTAGCTTCGCCGAGGATTTACCGGAGCTATGATTAGCGCAATCAGAGATATAGTCTGAGGGAAAAACAGCAAATTTATTCAACAAGGCGATAACCTGCTCTGGGGCTTCCTCCATGTTTGCTTTAAAGGTATTGGCTCCATGGTc                                                                                          >  1:98785/1‑251 (MQ=255)                                                                                                                                                           aTTATATAATGCCAGGCCAGTTCTTCTTCAATTGTCCCGTTTTGAAAAGCTGTGCTTGATATCGAGATCATCCATGATAATTCTGCCGCCCATATTAGCTTCGCCGAGGATTTACCGGAGCTATGATTAGCGCAATCAGAGATATAGTCTGAGGGAAAAACAGCAAATTTATTCAACAAGGCGATAACCTGCTCTGGGGCTTCCTCCATGTTTGCTTTAAAGGTATTGGCTCCATGGTCGCCagaaagaa                                                                               >  2:581542/1‑250 (MQ=255)                                                                                                                                                                                      tcAATTGTCCCGTTTTGAAAAGCTGTGCTTGATATCGAGATCATCCATGATAATTCTGCCGCCCATATTAGCTTCGCCGAGGATTTACCGGAGCTATGATTAGCGCAATCAGAGATATAGTCTGAGGGAAAAACAGCAAATTTATTCAACAAGGCGATAACCTGCTCTGGGGCTTCCTCCATGTTTGCTTTAAAGGTATTGGCTCCATGGTCGCCAGAAAGAAAATGCTCCATTAAGGCACAATAACTTTc                                                   >  2:507720/1‑251 (MQ=255)                                                                                                                                                                                          ttGTCCCGTTTTGAAAAGCTGTGCTTGATATCGAGATCATCCATGATAATTCTGCCGCCCATATTAGCTTCGCCGAGGATTTACCGGAGCTATGATTAGCGCAATCAGAGATATAGTCTGAGGGAAAAACAGCAAATTTATTCAACAAGGCGATAACCTGCTCTGGGGCTTCCTCCATGTTTGCTTTAAAGGTATTGGCTCCATGGTCGCCAGAAAGAAAATGCTCCATTAAGGCACAATAACTTTCGCt                                                >  2:491245/1‑250 (MQ=255)                                                                                                                                                                                              cccGTTTTGAAAAGCTGTGCTTGATATCGAGATCATCCATGATAATTCTGCCGCCCATATTAGCTTCGCCGAGGATTTACCGGAGCTATGATTAGCGCAATCAGAGATATAGTCTGAGGGAAAAACAGCAAATTTATTCAACAAGGCGATAACCTGCTCTGGGGCTTCCTCCATGTTTGCTTTAAAGGTATTGGCTCCATGGTCGCCAGAAAGAAAATGCTCCATTAAGGCACAATAACTTTCGCTATCtt                                           <  1:70117/251‑1 (MQ=255)                                                                                                                                                                                                      gAAAAGCTGTGCTTGATATCGAGATCATCCATGATAATTCTGCCGCCCATATTAGCTTCGCCGAGGATTTACCGGAGCTATGATTAGCGCAATCAGAGATATAGTCTGAGGGAAAAACAGCAAATTTATTCAACAAGGCGATAACCTGCTCTGGGGCTTCCTCCATGTTTGCTTTAAAGGTATTGGCTCCATGGTCGCCAGAAAGAAAATGCTCCATTAAGGCACAATAACTTTCGCTATCTTCGATAcc                                    <  1:507720/250‑1 (MQ=255)                                                                                                                                                                                                                     atatCGAGATCATCCATGATAATTCTGCCGCCCATATTAGCTTCGCCGAGGATTTACCGGAGCTATGATTAGCGCAATCAGAGATATAGTCTGAGGGAAAAACAGCAAATTTATTCAACAAGGCGATAACCTGCTCTGGGGCTTCCTCCATGTTTGCTTTAAAGGTATTGGCTCCATGGTCGCCAGAAAGAAAATGCTCCATTAAGGCACAATAACTTTCGCTATCTTCGATACCCCATTGATCCTCTaa                     >  1:321140/1‑250 (MQ=255)                                                                                                                                                                                                                           agaTCATGCATGATAAATCTGCCGCCCATATTAGATTAGCCGAGGATTTACCAGAGCTATGAATAGCGCAATCAGAGATATAGTCTGAGGGAAAAACAGCAAATTTATTCAACAAGGCGATAACCTGCTCTGGGGCTTCCTCCATGTTTGCTTTAAAGGTATTGGCTCCATGGTCGTCAGAAAGAAAATGCTCCATTAAGGCACAATAACGTGCGCTATCTTCGATACCCCATTGATCCTCTAACGACTcg              <  2:103621/251‑1 (MQ=255)                                                                                                                                                                                                                                       aTAATTCTGCCGCCCATATTAGCTTCGCCGAGGATTTACCGGAGCTATGATTAGCGCAATCAGAGATATAGTCTGAGGGAAAAACAGCAAATTTATTCAACAAGGCGATAACCTGCTCTGGGGCTTCCTCCATGTTTGCTTTAAAGGTATTGGCTCCATGGTCGCCAGAAAGAAAATGCTCCATTAAGGCACAATAACTTTCGCTATCTTCGATACCCCATTGATCCTCTAAAGACTCGCGTCTTTTACtt  <  1:422884/251‑1 (MQ=255)                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                    AAACTGCTTGTCGTAACGATAACACGCTTCAAGTTCAGCATCCGTTAACTTTCTGCGATAGCAGCAGATATGCCAGTAAAGAAATCCCATTTGACTATTTTTTTGATAATCTTCTTCGCTTTCGAACAACTCGTGCGCCTTTCGAGAAGCAAGCATTATATAATGCCAGGCCAGTTCTTCTTCAATTGTCCCGTTTTGAAAAGCTGTGCTTGATATCGAGATCATCCATGATAATTCCGCCGCCCATATTAGCTTCGCCGAGGATTTACCGGAGCTATGATTAGCGCAATCAGAGATATAGTCTGAGGGAAAAACAGCAAATTTATTCAACAAGGCGATAACCTGCTCTGGGGCTTCCTCCATGTTTGCTTTAAAGGTATTGGCTCCATGGTCGCCAGAAAGAAAATGCTCCATTAAGGCACAATAACTTTCGCTATCTTCGATACCCCATTGATCCTCTAAAGACTCGCGTCTTTTACTT  >  NC_000913/1843909‑1844389

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑