Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,828,654	C→T	G361R (GGG→AGG)	astD ←	aldehyde dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,828,654	0	C	T	96.5%	183.9 / NA	59	G361R (GGG→AGG)	astD	aldehyde dehydrogenase
Reads supporting (aligned to +/- strand):  ref base C (1/1);  new base T (37/18);  total (38/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATGGCGCGGTACTGGCAGCACCGGTAAGCGGTTTGTTCCAGTTAACAATCCCCGCCCGCGCCTCCAGCAACAGTTGATCGAACTTTTCCCGCTCGGGGGAAACCAGACCGCAAGAGAGTCCGAAGCGAGTGTTATTCGCCATTCGAATCGCTTCATCGAAAGTATCATAACGCCAGACGCGCAATAACGGTCCGAACACCTCTTCATCTGGTACGCCAGCAACGCCTGTCATTTCAATGATCCCCGGCGTCAGCAACGATGTCCCTGCTTGTAATAAGCGCGGCGCAAGCAGGG‑GTCGTCCGCCCATCGCTTCCAGTTGCTGCCATGCAGTAACCACCTGCTGTGCGGCCTGTTCAGAAATCAGCCCGCCAATAAACGGCTGCGGTTCGTCATCCCAGTTGCCCGGCGTTAATCGCTGGCTGACGGCAACCAGACGAGCAAGAAACGCATCGCCCTGCGCCCCGCTTTTCAGCAATAAACGGCGGGCGC  >  NC_000913/1828411‑1828899                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                       atgtcgcgGTACTGGCAGCCCCGGTAAGCGGTTTGTTCCATTTAACAATCCCCGCCCGCGCCTCCAGCAACAGTTGATCTATCTTTTCCCGCTCGGGGGAAACCAGACCGCAAGAGAGTCCGACGCTAGTGTTATTCGCCATTCTAATCGCTTCATCTAAAGTATCATAACTCCTGACTCGCAATAACGGTCCGAACACCTCTTCATCGGGTACGCCATCAACGCCTGTCATTTCAATTATCCTCGGCGTc                                                                                                                                                                                                                                                 <  1:499224/247‑1 (MQ=255)      gcgGTACTGGCAGCCCCGGTAAGCGGTTTGTTCCAGTTAACAATCCCCGCCCGCGCCTCCAGCAACAGTTGATCGAACTTTTCCCGCTCGGGGGAAACCAGACCGCAAGAGAGTCCGAAGCGAGTGTTATTCGCCATTCGAATCGCTTCATCGAAAGTATCATAACGCCAGACGCGCAATAACGGTCCGAACACCTCTTCATCTGGTACGCCAGCAACGCCTGTCATGTCAATGATCCTCGGCGTCAGCaa                                                                                                                                                                                                                                            <  2:245367/251‑1 (MQ=255)        ggTACTGGCAGCACCGGTAAGCGGTTTGTTCCAGTTAACAATCCCCGCCCGCGCCTCCAGCAACAGTTGATCGAACTTTTCCCGCTCGGGGGAAACCAGACCGCAAGAGAGTCCGAAGCGAGTGTTATTCGCCATTCGAATCGCTTCATCGAAAGTATCATAACGCCAGACGCGCAATAACGGTCCGAACACCTCTTCATCTGGTACGCCAGCAACGCCTGTCATTTCAATGATCCTCGGCGTCAGCAACg                                                                                                                                                                                                                                          >  1:122604/1‑251 (MQ=255)           aCTGGCAGCACCGGTAAGCGGTTTGTTCCAGTTAACAATCCCCGCCCGCGCCTCCAGCAACAGTTGATCGAACTTTTCCCGCTCGGGGGAAACCAGACCGCAAGAGAGTCCGAAGCGAGTGTTATTCGCCATTCGAATCGCTTCATCGAAAGTATCATAACGCCAGACGCGCAATAACGGTCCGAACACCTCTTCATCTGGTACGCCAGCAACGCCTGTCATTTCAATGATCCTCGGCGTCAGCAACGAt                                                                                                                                                                                                                                        <  1:373831/250‑1 (MQ=255)              ggCAGCACCGGTAAGCGGTTTGTTCCAGTTAACAATCCCCGCCCGCGCCTCCAGCAACAGTTGATCGAACTTTTCCCGCTCGGGGGAAACCAGACCGCAAGAGAGTCCGAAGCGAGTGTTATTCGCCATTCGAATCGCTTCATCGAAAGTATCATAACGCCAGACGCGCAATAACGGTCCGAACACCTCTTCATCTGGTACGCCAGCAACGCCTGTCATTTCAATGATCCTCGGCGTCAGCAACGATGTcc                                                                                                                                                                                                                                    >  1:139857/1‑251 (MQ=255)                cagcaCCGGTCAGATGTTTGTGCAAGTTAACAAGCCCCGCCCGGTCCTCCAGCAACAGTTGATCGAACTTTTCCCGCTCGTGGGAAACCAGACCGCCAGAGAGTCCGAAGCGAGTGTTATTCGCCATTCGAATCGCTTCAGCGAAAGTATCATAACGCCAGACGCGCAATACCGGTCCGAACACCTCTGCATCTGGTACGCCAGCAACGCCTGTCATTTCAATGATCCTCGTCGTCAGCAACGATGTcctg                                                                                                                                                                                                                                  <  2:144198/251‑3 (MQ=255)                cagcaCCGGTAAGCGGTTTGTTCCAGTTAACAATCCCCGCCCGCGCCTCCAGCAACAGTTGATCGAACTTTTCCCGCTCGGGGGAAACCAGACCGCAAGAGAGTCCGAAGCGAGTGTTATTCGCCATTCGAATCGCTTCATCGAAAGTATCATAACGCCAGACGCGCAATAACGGTCCGAACACCTCTTCATCTGGTACGCCAGCAACGCCTGTCATTTCAATGATCCTCGGCGTCAGCAACGATGTCCCt                                                                                                                                                                                                                                  >  1:104482/1‑251 (MQ=255)                 agcaCCGGTAAGCGGTTTGTTCCAGTTAACAATCCCCGCCCGCGCCTCCAGCAACAGTTGATCGAACTTTTCCCGCTCGGGGGAAACCAGACCGCAAGAGAGTCCGAAGCGAGTGTTATTCGCCATTCGAATCGCTTCATCGAAAGTATCATAACGCCAGACGCGCAATAACGGTCCGAACACCTCTTCATCTGGTACGCCAGCAACGCCTGTCATTTCAATGATCCTCGGCGTCAGCAACGATGTCCCTg                                                                                                                                                                                                                                 <  1:290471/251‑1 (MQ=255)                         tAAGCGGTTTGTTCCCGTTAACAATCCACGCCCGCGCCTCCAGCAACAGTTGATCGAACTTTTCCCGCTCGGGGGAAACCAGACCGCAAGAGAGTCCGAAGCGAGTGTTATTCGCCATTCGAATCGCTTCATCGAAAGTATCATAACGCCAGACGCGCACTAACGGTCCGAACACCTCTTCATCTGGTACGCCAGCAACGCCTGTCATTTCAATGATCCTCGGCGTCAGCAACGATGTCCCTGCTTGtaat                                                                                                                                                                                                                         >  2:349870/1‑251 (MQ=255)                           aGCGGTTTGTTCCAGTTAACAATCCCCGCCCGCGCCTCCAGCAACAGTTGATCGAACTTTTCCCGCTCGGGGGAAACCAGACCGCAAGAGAGTCCGAAGCGAGTGTTATTCGCCATTCGAATCGCTTCATCGAAAGTATCATAACGCCAGACGCGCAATAACGGTCCGAACACCTCTTCATCTGGTACGCCAGCAACGCCTGTCATTTCAATGATCCTCGGCGTCAGCAACGATGTCCCTGCTTGtaata                                                                                                                                                                                                                        >  1:432028/1‑250 (MQ=255)                             cattgtgtacaaGTTAACAAGCCGCGCCAGCGCCTCCAGCAACAGTTGAGCGAACTTTTCCCGCTCGGGAGAATCCAGACCGCAAGAGAGTCCGAAGCGAGTGTTGTGCGCTATTCGAATGGCTTCATCGAAAGTAGCATAACGTCAGACGCGCAATAACGGTCCGAACTCCTCTTCATCTGGTAGGCCAGCATCGTCTGTCATGCCGATGTGCCTCGGCTTCACCATCAATGTCCCTGCTTGGAATAAgc                                                                                                                                                                                                                     <  2:76774/240‑1 (MQ=255)                                    tgccatttAACAATCCCCGCCCGCGCCTCCAGCAACAGTTGATCGAACTTTTCCCGCTCGGGGGAAACCAGACCGCAAGAGAGGCCGAAGCGAGTGTTATTCGCCATTCGAATCGCTTCATCGAAAGTATCATAACGCCAGACGCGCAATAACGGTCCGAACTCCTCTTCATCTGGTACGCCAGCAACGCCTGTCATGTCAATGATCCTCGGCGTCAGCAACGATGTCCCTGCTTGTAATAAGCgctgcg                                                                                                                                                                                                               <  2:21219/244‑1 (MQ=255)                                     tCCAGTTAACAATCCCCGCCCGCGCCTCCAGCAACAGTTGATCGAACTTTTCCCGCTCGGGGGAAACCAGACCGCAAGAGAGTCCGAAGCGAGTGTTATTCGCCATTCGAATCGCTTCATCGAAAGTATCATAACGCCAGACGCGCAATAACGGTCCGAACACCTCTTCATCTGGTACGCCAGCAACGCCTGTCATTTCAATGATCCTCGGCGTCAGCAACGATGTCCCTGCTTGTAATAAGCGCGgcgc                                                                                                                                                                                                              >  1:274608/1‑250 (MQ=255)                                              cAATCCCCGCCCGCGCCTCCAGCAACAGTTGATCGAACTTTTCCCGCTCGGGGGAAACCAGACCGCAAGAGAGTCCGAAGCGAGTGTTATTCGCCATTCGAATCGCTTCATCGAAAGTATCATAACGCCAGACGCGCAATAACGGTCCGAACACCTCTTCATCTGGTACGCCAGCAACGCCTGTCATTTCAATGATCCTCGGCGTCAGCAACGATGTCCCTGCTTGTAATAAGCGCGGCGCAAGCAggg‑gg                                                                                                                                                                                                   >  1:553283/1‑250 (MQ=255)                                               aaTCCCCGCCCGCGCCTCCAGCAACAGTTGATCGAACTTTTCCCGCTCGGGGGAAACCAGACCGCAAGAGAGTCCGAAGCGAGTGTTATTCGCCATTCGAATCGCTTCATCGAAAGTATCATAACGCCAGACGCGCAATAACGGTCCGAACACCTCTTCATCTGGTACGCCAGCAACGCCTGTCATTTCAATGATCCTCGGCGTCAGCAACGATGTCCCTGCTTGTAATAAGCGCGGCGCAAGCAGGG‑gtc                                                                                                                                                                                                  <  1:296754/251‑1 (MQ=255)                                                  ccccGCCCGCGCCTCCAGCAACAGTTGATCGAACTTTTCCCGCTCGGGGGAAACCAGACCGCAAGAGAGTCCGAAGCGAGTGTTATTCGCCATTCGAATCGCTTCATCGAAAGTATCATAACGCCAGACGCGCAATAACGGTCCGAACACCTCTTCATCTGGTACGCCAGCAACGCCTGTCATTTCAATGATCCTCGGCGTCAGCAACGATGTCCCTGCTTGTAATAAGCGCGGCGCAAGCAGGG‑gtcgtc                                                                                                                                                                                               >  1:310067/1‑251 (MQ=255)                                                      gcccgcGCCTCCAGCAACAGTTGATCGAACTTTTCCCGCTCGGGGGAAACCAGACCGCAAGAGAGTCCGAAGCGAGTGTTATTCGCCATTCGAATCGCTTCATCGAAAGTATCATAACGCCAGACGCGCAATAACGGTCCGAACACCTCTTCATCTGGTACGCCAGCAACGCCTGTCATTTCAATGATCCTCGGCGTCAGCAACGATGTCCCTGCTTGTAATAAGCGCGGCGCAAGCAGGG‑GTCGTCCGcc                                                                                                                                                                                           >  2:428084/1‑251 (MQ=255)                                                         cgcgCCTCCAGCAAAAGTTGCTCGCACTTTTCCCGCACGGGGGAAACCCGACCGAAAGAGAGTCCGAAGCGAGTGTTATTCGCCATTCGAATCGCTTCATCGCAAGTATCATAACGCCAGACGCGCAATAACGGTCCGAACACCTCTTCATCTGGTACGCCAGCAACGCCTGTCATTTCAATGCTCTTCGGCGTCAGCAACGATGTCCCTGCTTGTAATAAGCGCGGCGCAAGCCGGG‑GACGCCCGCCCAt                                                                                                                                                                                        >  2:370308/1‑251 (MQ=255)                                                            gcCTCCAGCAACAGTTGATCGAACTTTTCCCGCTCGGGGGAAACCAGACCGCAAGAGAGTCCGAAGCGAGTGTTATTCGCCATTCGAATCGCTTCATCGAAAGTATCATAACGCCAGACGCGCAATAACGGTCCGAACACCTCTTCATCTGGTACGCCAGCAACGCCTGTCATTTCAATGATCCTCGGCGTCAGCAACGATGTCCCTGCTTGTAATAAGCGCGGCGCAAGCAGGG‑GTCGTCCGCCCATCGc                                                                                                                                                                                     <  1:57424/251‑1 (MQ=255)                                                             ccTCCAGCAACAGTTGATCGAACTTTTCCCGCTCGGGGGAAACCAGACCGCAAGAGAGTCCGAAGCGAGTGTTATTCGCCATTCGAATCGCTTCATCGAAAGTATCATAACGCCAGACGCGCAATAACGGTCCGAACACCTCTTCATCTGGTACGCCAGCAACGCCTGTCATTTCAATGATCCTCGGCGTCAGCAACGATGTCCCTGCTTGTAATAAGCGCGGCGCAAGCAGGG‑GTCGTCCGCCCATCGCt                                                                                                                                                                                    <  1:349870/251‑1 (MQ=255)                                                                   gCAACAGTTGATCGCACTTTTCCCGCTCGGGGGAAACCAGACCGCAAGAGAGTCCGAAGCGAGTGTTATTCGCCATTCGAATCGCTTCATCGAAAGTATCATAACGCCAGACGCGCAATAACGGTCCGAACACCTCTTCATCTGGTACGCCAGCAACGCCTGTCATTTCAATGATCCTCGGCGTCAGCAACGATGTCCCTGCTTGTAATAAGCGCGGCGCAAGCAGGG‑GTCGTCCGCCCATCGCTTCCAGt                                                                                                                                                                              >  1:434496/1‑251 (MQ=255)                                                                              tCGAACTTTTCCCGCTCGGGGGAAACCAGACCGCAAGAGAGTCCGAAGCGAGTGTTATTCGCCATTCGAATCGCTTCATCGAAAGTATCATAACGCCAGACGCGCAATAACGGTCCGAACACCTCTTCATCTGGTACGCCAGCAACGCCTGTCATTTCAATGATCCTCGGCGTCAGCAACGATGTCCCTGCTTGTAATAAGCGCGGCGCAAGCAGGG‑GTCGTCCGCCCATCGCTTCCAGTTGCTGCCATGc                                                                                                                                                                   >  2:32649/1‑251 (MQ=255)                                                                                gAACTTTTCCCGCTCGGGGGAAACCAGACCGCAAGAGAGTCCGAAGCGAGTGTTATTCGCCATTCGAATCGCTTCATCGAAAGTATCATAACGCCAGACGCGCAATAACGGTCCGAACACCTCTTCATCTGGTACGCCAGCAACGCCTGTCATTTCAATGATCCTCGGCGTCAGCAACGATGTCCCTGCTTGTAATAAGCGCGGCGCAAGCAGGG‑GTCGTCCGCCCATCGCTTCCAGTTGCTGCCATGCAg                                                                                                                                                                 <  1:291703/251‑1 (MQ=255)                                                                                      ttCCCGCTCGGGGGAAACCAGACCGCAAGAGAGTCCGAAGCGAGTGTTATTCGCCATTCGAATCGCTTCATCGAAAGTATCATAACGCCAGACGCGCAATAACGGTCCGAACACCTCTTCATCTGGTACGCCAGCAACGCCTGTCATTTCAATGATCCTCGGCGCCAGCAACGATGTCCCTGCTTGTAATAAGCGCGGCGCAAGCAGGG‑GTCGTCCGCCCATCGCTTCCAGTTGCTGCCATGCAGTAaccc                                                                                                                                                           >  2:463180/1‑250 (MQ=255)                                                                                         ccGCGCGGGGGAAACCAGACCGCAAGAGAGTCCGAAGCGAGTGTTATTCGCCATTCGAATCGCTTCATCGAAAGTATCATAACGCCAGACGCGCAATAACGGTCCGAACACCTCTTCATCTGGTACGCCAGCAACGCCTGTCATTTCAATGATCCTCGGCGTCAGCAACGATGTCCCTGCTTGTAATAAGCGCGGCGCAAGCAGGG‑GTCGTCCGCCCATCGCTTCCAGTTGCTGCCATGCAGTAACCACct                                                                                                                                                        >  2:203390/1‑251 (MQ=255)                                                                                            cTCGGGGGAAACCAGACCGCAAGAGAGTCCGAAGCGAGTGTTATTCGCCATTCGAATCGCTTCATCGAAAGTATCATAACGCCAGACGCGCAATAACGGTCCGAACACCTCTTCATCTGGTACGCCAGCAACGCCTGTCATTTCAATGATCCTCGGCGTCAGCAACGATGTCCCt                                                                                                                                                                                                                                  >  2:162927/1‑175 (MQ=255)                                                                                            cTCGGGGGAAACCAGACCGCAAGAGAGTCCGAAGCGAGTGTTATTCGCCATTCGAATCGCTTCATCGAAAGTATCATAACGCCAGACGCGCAATAACGGTCCGAACACCTCTTCATCTGGTACGCCAGCAACGCCTGTCATTTCAATGATCCTCGGCGTCAGCAACGATGTCCCt                                                                                                                                                                                                                                  <  1:162927/175‑1 (MQ=255)                                                                                             tCGGGGGAAACCAGACCGCAAGAGAGTCCGAAGCGAGTGTTATTCGCCATTCGAATCGCTTCATCGAAAGTATCATAACGCCAGACGCGCAATAACGGTCCGAACACCTCTTCATCTGGTACGCCAGCAACGCCTGTCATTTCAATGATCCTCGGCGTCAGCAACGATGTCCCTGCTTGTAATAAGCGCGGCGCAAGCAGGG‑GTCGTCCGCCCATCGCTTCCAGTTGCTGCCATGCAGTAACCACctgctg                                                                                                                                                    >  1:9071/1‑251 (MQ=255)                                                                                               gggggAAACCAGACCGCAAGAGAGTCCGAAGCGAGTGTTATTCGCCATTCGAATAGCTTCATCGAAAGTATCATAACGCCAGACGCGCAATAACGGTCCGAACACCTCTTCATCTGGTACGCCAGCAACGCCTGTCATTTCAATGATCCTCGGCGTCAGCAACGATGTCCCTGCTTGTAATAAGCGCGGCGCAAGCAGGG‑GTCGTCCGCCCATCGCTTCCAGTTGCTGCCATGCAGTAACCACCTGCtgtg                                                                                                                                                  >  2:253238/1‑251 (MQ=255)                                                                                                          gACCGCAAGAGAGTCCGAAGCGAGTGTTATTCGCCATTCGAATCGCTTCATCGAAAGTATCATAACGCCAGACGCGCAATAACGGTCCGAACACCTCTTCATCTGGTACGCCAGCAACGCCTGTCATTTCAATGATCCTCGGCGTCAGCAACGATGTCCCTGCTTGTAATAAGCGCGGCGCAAGCAGGG‑GTCGTCCGCCCATCGCTTCCAGTTGCTGCCATGCAGTAaccacc                                                                                                                                                         <  1:237527/233‑1 (MQ=255)                                                                                                          gACCGCAAGAGAGTCCGAAGCGAGTGTTATTCGCCATTCGAATCGCTTCATCGAAAGTATCATAACGCCAGACGCGCAATAACGGTCCGAACACCTCTTCATCTGGTACGCCAGCAACGCCTGTCATTTCAATGATCCTCGGCGTCAGCAACGATGTCCCTGCTTGTAATAAGCGCGGCGCAAGCAGGG‑GTCGTCCGCCCATCGCTTCCAGTTGCTGCCATGCAGTAaccacc                                                                                                                                                         >  2:237527/1‑233 (MQ=255)                                                                                                                        ccGAAGCGTGTGTTATTCGCCATTCGAATCGCTTCATCGAAAGTATCATAACGCCAGACGCGCAATAAAGGTCCGAACACCTCTTCATCTGGTACGCCAGCAACGCCTGTCATTTCAATGATCCTCGGCGTCAGCAACGATGTCCCTGCTTGTAATAAGCGCGGCGCAAGCAGGG‑GTCGTCCGCCCATCGCTTCCAGTTGCTGCCATGCAGTAACCACCTGCTGTGCGGCCTGTTCAGAAATCAGCCCGcc                                                                                                                         >  2:450124/1‑251 (MQ=255)                                                                                                                                        tCGCCATTCGAATCGCTTCATCGAAAGTATCATAACGCCAGACGCGCAATAACGGTCCGAACACCTCTTCATCTGGTACGCCAGCAACGCCTGTCATTTCAATGATCCTCGGCGTCAGCAACGATGTCCCTGCTTGTAATAAGCGCGGCGCAAGCAGGG‑GTCGTCCGCCCATCGCTTCCAGTTGCTGCCATGCAGTAACCACCTGCTGTGCGGCCTGTTCAGAAATCAGCCCGCCAATAAACGGCTGCGGt                                                                                                         >  2:406698/1‑251 (MQ=255)                                                                                                                                                               aaaGTATCATAACGCCAGACGCGCAATAACGGTCCGAACACCTCTTCATCTGGTACGCCAGCAACGCCTGTCATTTCAATGATCCTCGGCGTCAGCAACGATGTCCCTGCTTGTAATAAGCGCGGCGCAAGCAGGG‑GTCGTCCGCCCATCGCTTCCAGTTGCTGCCATGCAGTAACCACCTGCTGTGCGGCCTGTTCAGAAATCAGCCCGCCAATAAACGGCTGCGGTTCGTCATCCCAGTTGCCCGGCGt                                                                                  >  1:79927/1‑251 (MQ=255)                                                                                                                                                               aaaGTATCATAACGCCAGACGCGCAATAACGGTCCGAACACCTCTTCATCTGGTACGCCAGCAACGCCTGTCATTTCAATGATCCTCGGCGTCAGCAACGATGTCCCTGCTTGTAATAAGCGCGGCGCAAGCAGGG‑GTCGTCCGCCCATCGCTTCCAGTTGCTGCCATGCAGTAACCACCTGCTGTGCGGCCTGTTCAGAAATCAGCCCGCCAATAAACGGCTGCGGTTCGTCATCCCAGTTGCCCGGCGt                                                                                  >  2:450848/1‑251 (MQ=255)                                                                                                                                                                                  cgcgCAATAACGGTCCGAACACCTCTTCATCTGGTACGCCCGCAACGCCTGTCATTTCAATGATCCTCGGCGTCAGCAACGATGTCCCTGCTTGTAATAAGCGCGGCGCAAGCAGGG‑GTCGTCCGCCCATCGCTTCCAGTTGCTGCCATGCAGTAACCACCTGCTGTGCGGCCTGTTCAGAAATCAGCCCGCCAATAAACGGCTGCGGTTCGTCATCCCAGTTGCCCGGCGTTAATCGCTGGCTGACGGc                                                                >  2:46365/1‑250 (MQ=255)                                                                                                                                                                                  cgcgCAATAACGGTCCGAACAACTCTTCATCTGGCACGCCAGCAACGCCTGTCATTTCAATGATCCTCGGCGTCAGCAACGATGTCCCTGCTTGTAATAAGCGCGGCGCAAGCAGGG‑GTCGTCCGCCCATCGCTTCCAGTTGCTGCCATGCAGTAACCACCTGCTGTGCGGCCTGTTCAGAAATCAGCCCGCCAATAAACGGCTGCGGTTCGTCATCCCAGTTGCCCGGCGTTAATCGCTGGCTGACGGc                                                                >  2:406762/1‑250 (MQ=255)                                                                                                                                                                                     gcAATAACGGTCCGCACACCTCTTCATCTGGTACGCCAGCAACGCCTGTCATTTCAATGATCCTCGGCGTCAGCAACGATGTCCCTGCTTGTAATAAGCGCGGCGCAAGCAGGG‑GTCGTCCGCCCATCGCTTCCAGTTGCTGCCCTGCAGTAACCACCTGCTGTGCGGCCTGTTCAGAAATCAGCCCGCCAATAAACGGCTGCGGTTCGTCATCCCAGTTGCCAGGCGTTCATCGCTGGCTGACGGCCAcc                                                            >  2:227286/1‑251 (MQ=255)                                                                                                                                                                                     gcAATAACGGTCCGAACACCTCTTCATCTGGTACGCCAGCAACGCCTGTCATTTCAATGATCCTCGGCGTCAGCAACGATGTCCCTGCTTGTAATAAGCGCGGCGCAAGCAGGG‑GTCGTCCGCCCATCGCTTCCAGTTGCTGCCATGCAGTAACCACCTGCTGTGCGGCCTGTTCAGAAATCAGCCCGCCAATAAACGGCTGCGGTTCGTCATCCCAGTTGCCCGGCGTTAATCGCTGGCTGACGGCAAcc                                                            >  2:426405/1‑251 (MQ=255)                                                                                                                                                                                           actgTCCAAACCCCTCTTCATCTGGAACCCCAGCACCGCCTGTCATTTCAATGATCCTCGGCGTCAGCAACGATGTCCCGGCTTGTAATAAGCGCGGCGCAAGCAGGG‑GCCGTCCGCCCATCGCTTCCATTTGCTGCCATGCAGTAACCCCCTGCTGTGCGGCCTGTTCAAAAATCAGCCCGCCAATAAACGGCTGCGGTTCGTCATCCCAGTTGCCCGGCGTTAATCGCTGGCTGACGGCAACCAGACGa                                                      <  1:12963/248‑1 (MQ=255)                                                                                                                                                                                            cGGTCCGAACACCTCTTCATCTGGTACGCCAGCAACGCCTGTCATTTCAATGATCCTCGGCGTCAGCAACGATGTCCCTGCTTGTAATAAGCGCGGCGCAAGCAGGG‑GTCGTCCGCCCATCGCTTCCAGTTGCTGCCATGCAGTAACCACCTGCTGTGCGGCCTGTTCAGAAATCAGCCCGCCAATAAACGGCTGCGGTTCGTCATCCCAGTTGCCCGGCGTTAATCGCTGGCTGACGGCAACCAGACg                                                       >  2:146135/1‑249 (MQ=255)                                                                                                                                                                                             ggTCCGAACCCCTCGTCATCTGGTACCCCAGCAACGCCTGTCATTTCAATGACCCGCGGCGTCAGCAACGATGCCCCTTCTTGTAATAAGCGCGGCGCAAGCAGGG‑TCCGTCCGCCCATCTCTTCCAGTGGCTGCCATGCATTAACCCCCTGCTGGGCGGCGTTTTCAGAAATCATCCCGCCAATAAACGGCTGCGGTTCGTCATCCCAGTTGCCCGGCGTTAATCGCTGTCTGACGGCAACCAGACGAGc                                                    <  1:491517/251‑1 (MQ=255)                                                                                                                                                                                                            ccaccTGGTACGCCAGCAACGCCTGTCTTTTCAATGATCCTCGGCGGCAGCAACGATGCCCCTGCTTGAAATAAGCGCGGCGCAAGCGGGG‑GTCGTCCGCCCATCGCTTCCATTTGCTGCCATGCAGAAACCCCCGGCGGTGCGGCCTGTCCAAAAATCAGCCCGCCAATAAACGGCTGCGGTTCGTCATCCCAGTTGCCCGGCGTTAATCGCTGGCTGACGGCAACCAGACGAGCAAGAAACGCATCg                                       <  1:406698/245‑1 (MQ=255)                                                                                                                                                                                                              aTCTGGTACGCCAGCAACGCCTGTCATTTCAATGATCCTCGGCGTCAGCAACGATGTCCCTGCTTGTAATAAGCGCGGCGCAAGCAGGG‑GTCGTCCGCCCATCGCTTCCAGTTGCTGCCATGCAGTAACCACCTGCTGTGCGGCCTGTTCAGAAATCAGCCCGCCAATAAACGGCTGCGGTTCGTCATCCCAGTTGCCCGGCGTTAATCGCTGGCTGACGGCAACCAGACGAGCAAGAAACGCATCGCCCt                                   >  1:10325/1‑251 (MQ=255)                                                                                                                                                                                                                     aCGCCAGCACCCCCTGTCATTCCAATGATCCTCGGCGTCACCAACGAGGCCCCTTCTTGAAATAAGCGCGGCGCAAGCAGGG‑GTCGCCCGCCCATCGTTTCCAGTTGCTGCCATGCAGTAACCTCTTGCGGTGCGGCCTGTTCAGAAACCAGCCCGCCAATAAACGGCTGCGGTTCGTCATCCCAGTTGCCCGGCGTTAATCTCTGGCTGACGGCAACCAGACGAGCAAGAAACGCATCGCCCTGCGcccc                            <  1:46365/251‑1 (MQ=255)                                                                                                                                                                                                                         caccAACGCCTGTCATTCCAATGATCCCCGGCGTCAGCACCGATGCCCCTGCTTGTAAAAAGCGCGGCCCAAGCAGGG‑GCCGCCCGCCAACCTCTTCAATTGGCTGCCAGGCAGTACCCCCCTGCGGTGCGGCCTGTCAAAAAAGCGGCCCGCCAATAAACGGCTGCGGTTCGGCATCCCAGTGGCCCGGCGTTAATCGCTGGCTGACGGCAACCAGACGAGCAAGAAACGCATCGCCCTGCGCCCCGCtt                        <  2:93636/248‑1 (MQ=255)                                                                                                                                                                                                                          aGCAACGCCTGTCATTTCAATGATCCTCGGCGTCAGCAACGATGTCCCTGCTTGTAATAAGCGCGGCGCAAGCAGGG‑GTCGTCCGCCCATCGCTTCCAGTTGCTGCCATGCAGTAACCACCTGCTGTGCGGCCTGTTCAGAAATCAGCCCGCCAATAAACGGCTGCGGTTCGTCATCCCAGTTGCCCGGCGTTAATCGCTGGCTGACGGCAACCAGACGAGCAAGAAACGCATCGCCCTGCGCCCCGCttt                       >  2:181229/1‑251 (MQ=255)                                                                                                                                                                                                                          aGCAACGCCTGTCATTTCAATGATCCTCGGCGTCAGCAACGATGTCCCTGCTTGTAATAAGCGCGGCGCAAGCAGGG‑GTCGTCCGCCCATCGCTTCCAGTTGCTGCCATGCAGTAACCACCTGCTGTGCGGCCTGTTCAGAAATCAGCCCGCCAATAAACGGCTGCGGTTCGTCATCCCAGTTGCCCGGCGTTAATCGCTGGCTGACGGCAACCAGACGAGCAAGAAACGCATCGCCCTGCGCCCCGCttt                       >  2:467715/1‑251 (MQ=255)                                                                                                                                                                                                                           gCAACGCCTGTCATTTAAATGATCCTCGGCGTCAGCAACGATGTCCCTGCTTGTAATAAGCGCGGCGCAAGCAGGG‑TTCGTCCGCCCATCGCTTCCAGTTGCTCCCATGCAGTAACCACCTGCTGTGCGGCCTGTTCAGAAATCAGCCCGCCAATAAACGGCTGCGGTTCGTCATCCCAGTTGCCCGGCGTTAATCGCTGGCTGACGGCAACCAGACGAGCAAGAAACGCATCGCCCTGCGCCCCGCtttt                      <  1:224619/251‑1 (MQ=255)                                                                                                                                                                                                                             aaCGCCTGTCATTTCAATGATCCTCGGCGTCAGCAACGATGTCCCTGCTTGTAATAAGCGCGGCGCAAGCAGGG‑GTCGTCCGCCCATCGCTTCCAGTTGCTGCCATGCAGTAACCACCTGCTGTGCGGCCTGTTCAGAAATCAGCCCGCCAATAAACGGCTGCGGTTCGTCATCCCAGTTGCCCGGCGTTAATCGCTGGCTGACGGCAACCAGACGAGCAAGAAACGCATCGCCCTGCGCCCCGCTTTTc                     >  2:459749/1‑250 (MQ=255)                                                                                                                                                                                                                             aaCGCCTGGCATTTCACTGATCCCCGGCGTCAGCAACGATGTCCCTGCTTGTAATAAGCGCGGCGCAAGCAGCG‑GTCGTCCGCACATCGCTTCCAGTTGCTGCCATGCAGTAACCCCCTGCTGTGCGGCCTGATCAGAATTCACCCCGCCAATAAACGGCTGCGGTTCGTCATCCCAGTTGCCCGGCGTTAATCGCTGGCTGACGGCAACCCGCCGAGCAAGCAACGCATCGCCCTGCGCCCCGCttctc                     >  2:285602/1‑247 (MQ=255)                                                                                                                                                                                                                              aCGCCTGTCATTTCAATGATCCTCGGCGTCAGCAACGATGTCCCTGCTTGTAATAAGCGCGGCGCAAGCAGGG‑GTCGTCCGCCCATCGCTTCCAGTTGCTGCCATGCAGTAACCACCTGCTGTGCGGCCTGTTCAGAAATCAGCCCGCCAATAAACGGCTGCGGTTCGTCATCCCAGTTGCCCGGCGTTAATCGCTGGCTGACGGCAACCAGACGAGCAAGAAACGCATCGCCCTGCGCCCCGCTTTTc                     >  2:165067/1‑249 (MQ=255)                                                                                                                                                                                                                               cGCCTGTCATTTCAATGATCCTCGGCGTCAGCAACGATGTCCCTGATTGTCATAAGCGCGGCGCAAGCAGGG‑GTCGTCAGCCCATCGCTTCCAGTTGCTGCCATGCAGTAACCACCTGCTGTGCGGCCTGTTCAGAAATCAGCCCGCCAATAACCGGCTGCGGTTCGTCATCCCAGTTGCCCGGCGTTAATCGCTGGCTGACGGCAACCAGACGAGCAAGAAACGCATCGCCCTGCGCCCCGCTTTTCAGc                  >  1:384314/1‑251 (MQ=255)                                                                                                                                                                                                                                  cgttCATTCCAATGATCCGCGGCGCCAGCAACGATCCCCCTGTTTGTAATAGGCGCGGCGCAAGCAGGG‑GCCCTCCGCCCATCGTTTCCATTTGCTGCCATGCAGAAACCCCCTGTTGGGCGCCCTGTTAAAAAATCAGCCCGCCAATAAACGGCTGCGGTTCGTCATCCCAGTTGCCCGGCGTTAATCGCTGGCTGACGGCAACCAGACGAGCAAGAAACGCATCGCCCTGCGCCCCGCTTTTCAGCAAt               <  2:274608/248‑1 (MQ=255)                                                                                                                                                                                                                                    tttttttCAATGATCCTGGGCGCCAGCTACGTGGCCCCTGCTTGTAATAAGGGCGGCGAAAG‑AGGGTGTCGTCCGCCCACCGCTTCCAGTTGCGCCCATGCAGTAACCCCCTGCTGTGGGGCTTGTTCAGAAATCACCCCGCCAATAAACGGCTGCGGTTCGTCATCCCAGTTGCCCGGCGTTAATCGCTGGCTGACGGCAACCAGACGAGCAAGAAACGCATCGCCCTGCGCCCCGCTTTTCAGCAAt               <  2:553283/245‑1 (MQ=255)                                                                                                                                                                                                                                        tttCAATGATCATCGGCGTCAGCAACGATGTCCCTGCTTGTAATAAGCGCGGCGCAAGCAGGG‑GTCGTCCGCCCATCGCTTCCAGTTGCTGCCATGCAGTAACCACCTGCTGTGCGGCCTGTTCAGAAATCAGCCCGCCAATAAACGGCTGCGGTTCGTCATCCCAGTTGCCCGGCGTTAATCGCTGGCTGACGGCAACCAGACGAGCAAGAAACGCATCGCCCTGCGCCCCGCTTTTCAGCAATAAAcgg         >  1:152212/1‑251 (MQ=255)                                                                                                                                                                                                                                          tCAATGATCCTCGGCGTCAGCAACGATGTCCCTGCTTGTAATAAGCGCGGCGCAAGCAGGG‑GTCGACCGCCCAGCGCTTCCAGTTGCTGCCATGCAGTAACCACCTGCTGTGCGGCCTGTTCAGAAATCAGCCCGCCAATAAACGGCTGCGGTTCGTCATCCCAGTTGCCCGGCGTTAATCGCTGGCTGACGACAACCAGACGAGCAAGAAACGCATAGCCCTGCGCCCCGCTTTTCAGCAAAAAAcggcg       >  2:398192/1‑251 (MQ=255)                                                                                                                                                                                                                                              tGATCCTCGGCGTCAGCAACGATGTCCCTGCTTGTAATAAGCGCGGCGCAAGCAGGG‑GTCGTCCGCCCATCGCTTCCAGTTGCTGCCATGCAGTAACCACCTGCTGTGCGGCCTGTTCAGAAATCAGCCCGCCAATAAACGGCTGCGGTTCGTCATCCCAGTTGCCCGGCGTTAATCGCTGGCTGACGGCAACCAGACGAGCAAGAAACGCATCGCCCTGCGCCCCGCTTTTCAGCAATAAAAggcggg     >  2:510269/1‑249 (MQ=255)                                                                                                                                                                                                                                                aTCCTCGGCGTCAGCAACGATGTCCCTGCTTGTAATAAGCGCGGCGCAAGCAGGG‑GTCGTCCGCCCATCGCTTCCAGTTGCTGCCATGCAGTAACCACCTGCTGTGCGGCCTGTTCAGAAATCAGCCCGCCAATAAACGGCTGCGGTTCGTCATCCCAGTTGCCCGGCGTTAATCGCTGGCTGACGGCAACCAGACGAGCAAGAAACGCATCGCCCTGCGCCCCGCTTTTCAGCAATAAACGGCGGgcgc  >  2:225310/1‑250 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                       ATGGCGCGGTACTGGCAGCACCGGTAAGCGGTTTGTTCCAGTTAACAATCCCCGCCCGCGCCTCCAGCAACAGTTGATCGAACTTTTCCCGCTCGGGGGAAACCAGACCGCAAGAGAGTCCGAAGCGAGTGTTATTCGCCATTCGAATCGCTTCATCGAAAGTATCATAACGCCAGACGCGCAATAACGGTCCGAACACCTCTTCATCTGGTACGCCAGCAACGCCTGTCATTTCAATGATCCCCGGCGTCAGCAACGATGTCCCTGCTTGTAATAAGCGCGGCGCAAGCAGGG‑GTCGTCCGCCCATCGCTTCCAGTTGCTGCCATGCAGTAACCACCTGCTGTGCGGCCTGTTCAGAAATCAGCCCGCCAATAAACGGCTGCGGTTCGTCATCCCAGTTGCCCGGCGTTAATCGCTGGCTGACGGCAACCAGACGAGCAAGAAACGCATCGCCCTGCGCCCCGCTTTTCAGCAATAAACGGCGGGCGC  >  NC_000913/1828411‑1828899

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑