Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,812,346	C→T	G263R (GGA→AGA)	ydjO ←	protein YdjO

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,812,346	0	C	T	100.0%	104.5 / NA	34	G263R (GGA→AGA)	ydjO	protein YdjO
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (15/19);  total (15/19)

CGGTGGTGGTGCAACTTTCGCCGCACTGATTGTACTGCCTGCGATGGGCCTGCCAGTAACCCTGGTGGCGCTGTTAATCTCCGTTGAACCGCTTATCGACATGGGCCGTACGGCGTTAAACGTTAGTGGCTCGATGACAGCTGGCACGCTGACCAGCCAGTGGCTGAAGCAAACCGATAAAGCCATTCTGGATAGCGAAGACGACGCCGAACTGGCACACCATTAATTCTTATGCTGGCAACGGTCCGTTTTGTATAGGGGCCGTTGCCTTACTTTTTAAATGTTCCATCGCGGCGATGCATACTGACTCATATTTAAACAGATCGTCAGCGAAAAAAATGTCCAACTTATCAATACATTCCTGGCTCAATGAATGTGCCTGTAGCGAGAAGCTTAAAACCTCATACCCGAGACGTTTTCTAAGTGAACTATCTGGAATACTCATAATATCTTCAAGCACACCACTTCCCAT  >  NC_000913/1812100‑1812571                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                  gggggggggtgCAACTTTCCCCGCACTGTTTGTCCTGCCGGCGAAGGGCCTGCCAGTAACCCGGGTGGCGGTGTGAATCTCCGTTGAACCGCTTATCGACATGGGCCGTACGGCGTTAAACGTTAGTGGCACGATGACAGCTGGCACGCTGACCAGCCAGTGGCTGAAGCAAACCGATAAAGCCATTCTGGATAGCGAAGACGTCGCCGAACTGGCACACCATTAATTCTTATGCCGGCAACGGTCTGttt                                                                                                                                                                                                                               <  2:61706/244‑1 (MQ=255)        ggtgCAGCTTTCGCCGCAGTGATTGGACGGCCGGCTATGGGCCTGCCAGTAACCCTGGTGGGGCTGTTAATCGCCGTTGAACCGCTTATCGACATGGGCCGTACGGCGTTAAACGTTAGTGGCTCGATGACAGCTGGCACGCTGACCAGCCAGTGGCTGAAGCAAACCGATAAAGCCATTCTGGATAGCGAAGACGACGTCGAACTGGCACACCATTAATTCTTATGCTGGCAACGGTCTGTTTTGTATAg                                                                                                                                                                                                                        <  2:126357/251‑1 (MQ=255)             aaCTTTCGCCGCACTGATTGTACTGCCTGCGATGGGCCTGCCAGTAACCCTGGTGGCGCTGTTAATCTCCGTTGAACCGCTTATCGACATGGGCCGTACGGCGTTAAACGTTAGTGGCTCGATGACAGCTGGCACGCTGACCAGCCAGTGGCTGAAGCAAACCGATAAAGCCATTCTGGATAGCGAAGACGACGCCGAACTGGCACACCATTAATTCTTATGCTGGCAACGGTCTGTTTTGTATAGGGGcc                                                                                                                                                                                                                   <  1:462053/251‑1 (MQ=255)                 ttCGCCGCACTGATTGTACTGCCGGCGATGGGCCTGCCAGTAACCCTGGTGGCGCTGTTAATCTCCGTTGAACCGCTTATCGACATGGGCCGTACGGCGTTAAACGTTAGTGGCTCGATGACAGCTGGCACGCTGACCAGCCAGTGGCTGAAGCAAACCGATAAAGCCATTCTGGATAGCGAAGACGACGCCGAACTGGCGCACCATTAATTCTTATGCTGGCAACGGTCTGTTTTGTATAGGGGCCGtt                                                                                                                                                                                                                <  2:294564/250‑1 (MQ=255)                   cgccgcACTGATTGTACTGCCTGCGATGGGCCTGCCAGTAACCCTGGTGGCGCTGTTAATCTCCGTTGAACCGCTTATCGACATGGGCCGTACGGCGTTAAACGTTAGTGGCTCGATGACAGCTGGCACGCTGACCAGCCAGTGGCTGAAGCAAACCGATAAAGCCATTCTGGATAGCGAAGACGACGCCGAACTGGCACACCATTAATTCTTATGCTGGCAACGGTCTGTTTTGTATAGGGGCCGTTGc                                                                                                                                                                                                              <  1:577610/250‑1 (MQ=255)                      cgcACTGATTGTACTGCCTGCGATGGGCCTGCCAGTAACCCTGGTGGCGCTGTTAATCTCCGTTGAACCGCTTATCGACATGGGCCGTACGGCGTTAAACGTTAGTGGCTCGATGACAGCTGGCACGCTGACCAGCCAGTGGCTGAAGCAAACCGATAAAGCCATTCTGGATAGCGAAGACGACGCCGAACTTGCACACCATTAATTCTTATGCTGGCAACGGTCTGTTTTGTATAGGGGCCGTTGCCttt                                                                                                                                                                                                          >  2:514115/1‑250 (MQ=255)                           tGATTGTACTGCCTGCGATGGGCCTGCCAGTAACCCTGGTGGCGCTGTTAATCTCCGTTGAACCGCTTATCGACATGGGCCGTACGGCGTTAAACGTTAGTGGCTCGATGACAGCTGGCACGCTGACCAGCCAGTGGCTGAAGCAAACCGATAAAGCCATTCTGGATAGCGAAGACGACGCCGAACTGGCACACCATTAATTCTTATGCTTGCAACGGTCTGTTTTGTATCAGGGCCGTTGCCTTACtttt                                                                                                                                                                                                     >  2:21045/1‑251 (MQ=255)                             aTTGTACTGCCTGCGATGGGCCTGCCAGTAACCCTGGTGGCGCTGTTAATCTCCGTTGAACCGCTTATCGACATGGGCCGTACGGCGTTAAACGTTAGTGGCTCGATGACAGCTGGCACGCTGACCAGCCAGTGGCTGAAGCAAACCGATAAAGCCATTCTGGATAGCGAAGACGACGCCGAACTGGTACACCATTAATTCTTATGCTGGCAACGGTCTGGTTTGTATAGGGGCCGTTGCCTTACTTTTTa                                                                                                                                                                                                   <  1:438429/251‑1 (MQ=255)                                     gcctgcGATGGGCCTGCCAGTAACCCTGGTGGCGCTGTTAATCTCCGGTGAACCGCTTATCGACATGGGCCGTACGGCGTTAAACGTTAGTGGCTCGCTGACAGCTGGCAAGCTGAGCAGCCAGTGGCTGAAGCAAACCGATAAAGCCATTCTGGATAGCGAAAACGACGCCGAACTGGCACACCCTTAATTCTTATGCCGGCAACGGGCTGTTTTGTAAAGGGGCCGTTGCCTTACTTTTTAAATGTTcc                                                                                                                                                                                           >  1:249485/1‑251 (MQ=255)                                                 ccgtccAGTACCCCTGGTGGCGCTGTTAATCTCCGTTGAACCGCTTATCGACATGGGCCGTACGGCGTTAAACGTTAGTGGCTCGATGACAGCTGGCACGCTGACCAGCCAGTGGCTGAAGCAAACCGATAAAGCCATTCTGGATAGCGAAGACGACGCCGAACTGGCACACCATTAATTCTTATGCTGGCAACGGTCTGTTTTGTATAGGGGCCGTTGCCTTACTTTTTAAATGTTCCATCTCGGCGATg                                                                                                                                                                               <  2:323828/247‑1 (MQ=255)                                                   tGCCAGTAACCCTGGTGACGCTGTTAATCTCCGTTGAACCGCTTATCGACATGGGCCGTACGGCGTTAAACGTTAGTGGCTCGATGACCGCTGGCACGCTGACCAGCCAGTGGCTGCAGCAAACCGATAAAGCCATTCTGGATAGCGAAGACGACGCCGAACTGGCCCACCATTAATTCTTATGCTGGCCACGGTCTGTTTTGTATAGGGGCCGTTGCCTTACTTTTTAAATGTTCCCTCGCGGCGCTGc                                                                                                                                                                              >  2:271059/1‑250 (MQ=255)                                                          aaCCCTGGTGGCGCTGTTAATCTCCGTTGAACCGCTTATCGACATGGGCCGTACGGCGTTAAACGTTAGTGGCTCGATGACAGCTGGCACGCTGACCAGCCAGTGGCTGAAGCAAACCGATAAAGCCATTCTGGATAGCGAAGACGACGCCGAACTGGCACACCATTAATTCTTATGCTGGCAACGGTCTGTTTTGTATAGGGGCCGTTGCCTTACTTTTTAAATGTTCCATCGCGGCGATGCATACTGAc                                                                                                                                                                      <  1:126375/251‑1 (MQ=255)                                                             ccTGGTGGCGCTGTTAATCTCCGTTGAACCGCTTATCGACATGGGCCGTACGGCGTTAAACGTTAGTGGCTCGATGACAGCTGGCCCGCTGACCAGCCAGTGGCTGAAGCAAACCGATAAAGCCATTCTGGATAGCGAAGACGACGCCGAACTGGCACCCCATTAATTCTTATGCTGGCAACGGTCTGTTTTGTATATGGGCCGGTGCCTTACTTTTTAAATGTTCCATCGCGGCGATGCCTACTGtctc                                                                                                                                                                    >  1:114324/1‑250 (MQ=255)                                                              cTGGTGGCGCTGTTAATCTCCGTTGAACCGCTTATCGACATGGGCCGTACGGCGTTAAACGTTAGTGGCTCGATGACAGCTGGCACGCTGACCAGCCAGTGGCTGAAGCAAACCGATAAAGCCATTCTGGATAGCGAAGACGACGCCGAACTGGCCCAACATTAATTCTTATGCTGGCAACGGTCTGTTTTGGATAGGGGCCGTTGCCTTACTTTTTAAATGTTCCATCGCGGCGATGCCTAATGCCTCat                                                                                                                                                                  >  1:199728/1‑251 (MQ=255)                                                                     cgttGTTAATCTCCGTTGAACCGCTTATCGACATGGGCCGTACGGCGTTAAACTTTAGTGGCTCGATGACAGCTGGCACGCTGACCAGCCAGTGGCTGAAGCAAACCGATAAAGCCATTCTGGATAGCGAAGACGACGCCGAACTGGCACACCATTAATTCTTATGCTGGCAACGGTCTGTTTTGTATAGGGGCCGTTGCCTTACTTTTTAAATGTTCCATCGCGGCGATGCATACTGACTCATATTTaaa                                                                                                                                                           <  2:583858/248‑1 (MQ=255)                                                                                 ccGTTTAACCGCTTACCGACATGGGCCGTACGGCGTGAAACGTTAGTGGATCGATGACAGCTGGCACGCTGACCAGCCAGTGGCTGAAGCAAACCGATAAAGCCATTCTGGATAGCGAAGACGACGCCGAACTGGCACACCATTAATTCTTATGCTGTCAACGGTCTGTTTTGTATAGGGGCCGTTGCCTTACTTTTTAAATGTTCCATCGCGGCGATGCATACTGACTCATATTTAAACAGATCGt                                                                                                                                                   <  2:309638/247‑1 (MQ=255)                                                                                              tATCGACATGGGCCGTACGGCGTTAAACGTTAGTGGCTCGATGACAGCTGGCACGCTGACCAGCCAGTGGCTGAAGCAAACCGATAAAGCCATTCTGGATAGCGAAGACGACGCCGAACTGGCACACCATTAATTCTTATGCTGGCAACGGTCTGTTTTGTATAGGGGCCGTTGCCTTACTTTTTAAATGTTCCATc                                                                                                                                                                                        >  1:225703/1‑197 (MQ=255)                                                                                              tATCGACATGGGCCGTACGGCGTTAAACGTTAGTGGCTCGATGACAGCTGGCACGCTGACCAGCCAGTGGCTGAAGCAAACCGATAAAGCCATTCTGGATAGCGAAGACGACGCCGAACTGGCACACCATTAATTCTTATGCTGGCAACGGTCTGTTTTGTATAGGGGCCGTTGCCTTACTTTTTAAATGTTCCATc                                                                                                                                                                                        <  2:225703/197‑1 (MQ=255)                                                                                               aTCGACATGGGCCGTACGGCGTTAAACGTTAGTGGCTCGATGACAGCTGGCACGCTGCCCAGCCAGTGGCTGAAGCAAACCGATAAAGCAATTCTGGATAGCGACGACGACTCCGCACTGGCACCCCATTAATTCTTATGCTGGCAACGGTCTGTTTTGTATAGGGGCCGTTGCCTTACTTTTTAAATGTTCCATCGCGGCGATGCATACTGACTCCTATTTAAACAGATAGACAGCGACAAAAATGTcc                                                                                                                                  >  2:307032/1‑250 (MQ=255)                                                                                                   aCATGGGCCGTACGGCGTTAAACGTTAGTGGCTCGATGACAGCTGGCACGCTGACCAGCCAGTGGCTGAAGCAAACCGATAAAGCCATTCTGGATAGCGAAGACGACGCCGAACTGGCACACCATTAATTCTTATGCTGGAAACGGTCTGTTTTGTATAGGGGCCGTTGCCTTACTTTTTAAATGTTCCATCGCGGCGATGCATACTGACTCATATTTAAACAGATCGTCAGCGAAAAAAATGTCCAACtt                                                                                                                             >  2:458830/1‑251 (MQ=255)                                                                                                       gggCCGTACGGCGTTAAACGTTAGTGGCTCGATGACAGCTGGCACGCTGACCAGCCAGTGGCTGAAGCAAACCGATAAAGCCATTCTGGATAGCGAAGACGACGCCGAACTGGCACACCATTAATTCTTATGCTGGCAACGGTCTGTTTTGTATAGGGGCCGTTGCCTTACTTTTTAAATGTTCCATCGCGGCGATGCATACTGACTCATATTTAAACAGATCGTCAGCGAAAAAAATGTCCAACTTATCa                                                                                                                         <  2:182159/251‑1 (MQ=255)                                                                                                        ggCCGTACGGCGTTAAACGTTAGTGGCTCGATGACAGCTGGCACGCTGACCAGCCAGTGGCTGAAGCAAACCGATAAAGCCATTCTGGATAGCGAAGACGACGCCGAACTGGCACACCATTAATTCTTATGCTGGCAACGGTCTGTTTTGTATAGGGGCCGTTGCCTTACTTTTTAAATGTTCCATCGCGGCGATGCATACTGACTCATATTTAAACAGATCGTCAGCGAAAAAAATGTCCAACTTTTCa                                                                                                                         >  2:122959/1‑250 (MQ=255)                                                                                                                                 gCTCGATGACAGCTGGCACGCTGACCAGCCAGTGGCTGAAGCAAAACGATAACGCCATTCTGGATAGCGAAGACGACGCCGAACTGGCACACCATTAATTCTTATGCTGGCAACGGTCTGTTTTGGATAGGGGCCGTTGCCTTACTTTTTAAATGTTCAATCGCGGCGATGCATACTGACTCCTATTTAAACAGATCGTCAGCGAAAAAAATGTCCAACTTATCAATACATTCCTGGCTCAATGAATGTGc                                                                                               >  2:556061/1‑251 (MQ=255)                                                                                                                                    cGATGACAGCTGGCACGCTGACCAGCCAGTGGCTGAAGCAAACCGATAAAGCCATTCTGGATAGCGAAGACGACGCCGAACTGGCACACCATTAATTCTTATGCTGGCAACGGTCTGTTTTGTATAGGGGCCGTTGCCTTACTTTTTAAATGTTCCATCGCGGCGATGCATACTGACTCATATTTAAACAGATCGTCAGCGAAAAAAATGTCCAACTTATCAATACATGCCTGGCTCAATGAATGTGCCt                                                                                             <  1:122959/250‑1 (MQ=255)                                                                                                                                                    gCTGACCAGCCAGTGGCTGAAGCAAACCGATAAAGCAATTCTGGATAGCGAAGACGACGCCGAACTGGCACACCATTAATTCTTATGCTGGCAACGGTCTGTTTTGTATAGGGGCCGTTGCCTTACTTTTTAAATGTTCCATCGCGGCGATGCATACTGACTCATATTTAAACAGATAGTCAGCGAAAAAAATGTCCAACTTATCAATACATTCCTGGCTCAATGAATGTGCCTGTAGCGAGAAGCTTaa                                                                             >  2:176416/1‑250 (MQ=255)                                                                                                                                                         ccagccagTGGCTGAAGCAAACCGATAAAGCCATTCTGGATAGCGAAGACGACGCCGAACTGGCACACCATTAATTCTTATGCTGGCAACGGTCTGTTTTGTATAGGGGCCGTTGCCTTACTTTTTAAATGTTCCATCGCGGCGATGCATACTGACTCATATTTAAACAGATCGTCAGCGAAAAAAATGTCCAGCTTATCAATACATTCCTGGCTCAATGAATGTGCCTGTAGCGAGAAGCTTAAAACCTc                                                                       >  2:231546/1‑251 (MQ=255)                                                                                                                                                         ccagccagTGGCTGAAGCAAACCGATAAAGCCATTCTGGATAGCGAAGACGACGCCGAACTGGCACACCATTAATTCTTATGCTGGCAACGGTCTGTTTTGTATAGGGGCCGTTGCCTTACTTTTTAAATGTTCCATCGCGGCGATGCATACTGACTCATATTTAAACAGATCGTCAGCGAAAAAAATGTCCAACTTATCAATACATTCCTGGCTCAATGAATGTGCCTGTAGCGAGAAGCTTAAAACCTc                                                                       >  2:135359/1‑251 (MQ=255)                                                                                                                                                             ccagTGGCTGAAGCAAACCGATAAAGCCATTCTGGATAGCGAAGACGACGCCGAACTGGCACACCATTAATTCTTATGCTGGCAACGGTCTGTTTTGTATAGGGGCCGTTGCCTTACTTTTTAAATGTTCCATCGCGGCGATGCATACTGACTCATATTTAAACAGATCGTCAGCGAAAAAAATGTCCAACTTATCAATACATTCCTGGCTCAATGAATGTGCCTGTAGCGAGAAGCGTAAAACCTCATAc                                                                   <  1:458830/251‑1 (MQ=255)                                                                                                                                                                  ggCTGAAGCAATCCGAGAAAGCCATGCGGGATTGCGGAGACGACGCCGAACTGGCACACCATTAATTCTTATGCTTGCAACGGTCTGGTTTGTATAGTGTTCGTTGCCTTACTTTTTAAATTTTCCAACGCGGCGATCCATACTTACGCAGATTTAAACTGATCGTCAGCGAAAAAAATGGCCAAGTTATCAATACATTCGGGGCGCAATGAATGTGCCTGTAGCGAGAAGCTTAAAACCTCATACCCgag                                                              <  1:241986/251‑1 (MQ=255)                                                                                                                                                                     tGAAGCAAACCGATACAGCCATTCTGGATAGCGAAGACGACGCCGAACTGGCACACCATTAATTCTTATGCTGGCAACGGTATGTTTTGTATAGGGGCCGTTGCCTTACTTTTTAAATGTTCCATCGCGGCGATGCATACTGACTCATATTTAAACAGATCGTCAGCGAAAAAAATGTCCAACTTATCAATACATTCCTGGCTCAATGAATGTGCCTGTAGCGAGAAGCTTAAAACCTCATACCCGAGACg                                                           >  2:482655/1‑251 (MQ=255)                                                                                                                                                                       aaGCAAACCGATAAAGCCATTCTGGATAGCGAAGACGACGCCGAACTGGCACACCATTAATTCTTATGCTGGCAACGGTCTGTTTTGTATAGGGGCCGTTGCCTTACTTTTTAAATGTTCCATCGCGGCGATGCATACTGACTCATATTTAAACAGATCGTCAGCGAAAAAAATGTCCAACTTATCAATACATTCCTGGCTCAATGAATGTGCCTGTAGCGAGAAGCTTAAAACCTCATACCCGAGACGtt                                                         <  1:473154/251‑1 (MQ=255)                                                                                                                                                                                              ggATAGCGAAGAAGACGCCGAACTGGCACACCATTAATTCTTATGCTGGCAACGGTCTGTTTTGTATAGGGGCCGTTGCCTTACTTTTTAAATGTTCCATCGCGGCGATGCATACTGACTCATATTTAAACAGATCGTCAGCGAAAAAAATGTCCAACTTATCAATACATTCCTGGCGCAATGAATGTGCCTGTAGCGAGAAGCTTAAAACCTCATACCCGAGACGTATTCTAAGTGAACGATCTGGAATa                                  <  2:247768/251‑1 (MQ=255)                                                                                                                                                                                                                           cccATTAATTCTTATGCTGGCAACGGTCTGTTTGGTATAGGGGCCGTTGCCTTACTTTTTAAATGTTCCAGCGCGGCGATGCATACTGACTCATATTTAAACAGAGCGTCAGCGAAAAAAATGTCCAACTTAACAATACATTCCTGGCTCAATGAATGTGCCTGTAGCGAGAAGCTTAAAACCTCATACCCGAGACGTTTTCTAAGTGAACTATCTGGAATACTCATAATATCTTCAAGCACACCACTTcc     <  2:35613/250‑1 (MQ=255)                                                                                                                                                                                                                              aTTAATTCTTATGCTGGCAACGGTCTGTTTTGTATAGGGGCCGTTGCCTTACTTTTTAAATGTTCCATCGCGGCGATGCATACTGACTCATATTTAAACAGATCGTCAGCGAAAAAAATGTCCAACTTATCAATACATTCCTGGCTCAATGAATGTGCCTGTAGCGAGAAGCTTAAAACCTCATACCCGAGACGTTTTCTAAGTGAACTATCTGGAATACTCATAATATCTTCAAGCACACCACTTCCCAt  <  1:40305/251‑1 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                  CGGTGGTGGTGCAACTTTCGCCGCACTGATTGTACTGCCTGCGATGGGCCTGCCAGTAACCCTGGTGGCGCTGTTAATCTCCGTTGAACCGCTTATCGACATGGGCCGTACGGCGTTAAACGTTAGTGGCTCGATGACAGCTGGCACGCTGACCAGCCAGTGGCTGAAGCAAACCGATAAAGCCATTCTGGATAGCGAAGACGACGCCGAACTGGCACACCATTAATTCTTATGCTGGCAACGGTCCGTTTTGTATAGGGGCCGTTGCCTTACTTTTTAAATGTTCCATCGCGGCGATGCATACTGACTCATATTTAAACAGATCGTCAGCGAAAAAAATGTCCAACTTATCAATACATTCCTGGCTCAATGAATGTGCCTGTAGCGAGAAGCTTAAAACCTCATACCCGAGACGTTTTCTAAGTGAACTATCTGGAATACTCATAATATCTTCAAGCACACCACTTCCCAT  >  NC_000913/1812100‑1812571

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑