Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,795,562	C→T	D795N (GAT→AAT)	pheT ←	phenylalanine‑‑tRNA ligase subunit beta

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,795,562	0	C	T	100.0%	103.3 / NA	32	D795N (GAT→AAT)	pheT	phenylalanine‑‑tRNA ligase subunit beta
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (21/11);  total (21/11)

GTGCTGTAATGGGAATATCCTCGCCCGTTTTCGGGTTACGTCCCGGGCGTTGATTCTTATCACGCAGATCGAAGTTACCAAAACCAGAGAGTTTCACCTGTTCGCCGTTTTCCAGAGCGCGACGGATCTCTTCGAAAAACAGTTCAACCAGTTCTTTGGCATCCCGCTTGCTAAGCCCAAGCTTATCAAACAGATATTCTGACATTTCAGCTTTTGTAAGCGCCATAGGTTCAATCCCTCAATGATGCCTGGAATCGCTCTTTTAATGCCTCTACACATTTGGCGACGGTAGCGGCAATCTCCTCTTCTTCGAGTGTACGGCTGGTATCTTGCAGGATCAGGCTTATGGCGAGGCTCTTATACCCCTCCGCAACACCCTTACCGCGGTACACGTCAAATAAGTTTACGCCAACTACCTGATTTACGCCAACTTTCTTACATTCGGATAAAATATCCGCTGCGGGAACGT  >  NC_000913/1795327‑1795795                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                          gTGCTGTAATGGGAATATCCTCGCCCGTTTTCGGGTTACGTCCCGGGCGTTGATTCTTATCACGCAGATCGAAGTTACCAAAACCAGAGAGTTTCACCTGTTCGCCGTTTTCCAGAGCGCGACGGATCTCTTCGAAAAACAGTTCAACCAGTTCTTTGGCATCCCGCTTGCTAAGCCCAAGCTTATCAAACAGATATTCTGACATTTCAGCTTTTGTAAGCGCCATAGGTTCAATTCCTCAATGATGCCTg                                                                                                                                                                                                                            >  1:176802/1‑251 (MQ=255)     ggTAATGGGAATATCCTCGCCCGTTTTCGGGTTACGTCCCGGGCGTTTATTCTTATCACGCAGATCGAAGTTACCAAAACCAGAGAGTTTCACCTGTTCGCCGTTTTCCAGAGCGCGACGGATCTCTTCGAAAAACAGTTCAACCAGTTCTTTGGCATCCCGCTTGCTAAGCCCAAGCTTATCAAACAGATATTCTGACATTTCAGCTTTTGTAAGCGCCATGGGTTCAATTCCTCAATGATGCCTGGAAt                                                                                                                                                                                                                        <  2:42204/250‑1 (MQ=255)         aTGGGAATATCCTCGCCCGTTTTCGGGTTACGTCCCGGGCGTTGATTCTTATCACGCAGATCGAAGTTACCAAAACCAGAGAGTTTCACCTGTTCGCCGTTTTCCAGAGCGCGACGGATCTCTTCGAAAAACAGTTCAACCAGTTCTTTGGCATCCCGCTTGCTAAGCCCAAGCTTATCAAACAGATATTCTGACATTTCAGCTTTTGTAAGCGCCATAGGTTCAATTCCTCAATGATGCCTGGAATCGct                                                                                                                                                                                                                    >  1:322131/1‑251 (MQ=255)                         ccGTTTTCGGGTTACGTACCGGGCGTGGATTCTTATCACGCAGATCGAAGTTACCAAAACCAGAGAGTTTCACATGTTCGCTGTTTTCCAGAGCGCGACGGATCTCTTCGAAAAACAGTTCAACCAGTTCTTTGGCATCCCGCTTGCTAAGCCCAAGCTTATCAAACAGATATTCTGCCATTTCAGCTTTTGTAAGCGCCATAGGTTCAATTCCTCAATGATGCCTGGAATCGCTCTTTTAATGCCTCTac                                                                                                                                                                                                    >  2:263225/1‑251 (MQ=255)                           gTTTTCGGGTTACGTCCCGGGCGTTGATTCTTATCACGCAGATCGAAGTTACCAAAACCAGAGAGTTTCACCTGTTCGCCGTTTTCCAGAGCGCGACGGATCTCTTCGAAAAACAGTTCAACCAGTTCTTTGGCATCCCGCTTGCTAAGCCCAAGCTTATCAAACAGATATTCTGACATTTCAGCTTTTGTAAGCGCCATAGGTTCAATTCCTCAATGATGCCTGGAATCGCTCTTTTAATGCCTCTacac                                                                                                                                                                                                  >  1:118445/1‑251 (MQ=255)                                           ccGGGCGTTGCTTCTTATCACGCAGATCGAAGTTACCAAAACCAGAGAGTTTCACCTGTTCGCCGTTTTCCAGAGCGCGACGGATCTCTTCGAAAAACAGTTCAACCAGTTCTTTGGCATCCCGCTTGCTAAGCCCAAGCTTATCAAACAGATATTCTGACATTTCAGCTTTTGTAAGCGCCATAGGTTCAATTCCTCAATGATGCCTGGAATCGCTCTTTTAATGCCTCTACACATTTGGCGACGGTAGc                                                                                                                                                                                  >  2:376597/1‑251 (MQ=255)                                                   tGATTCTTATCACGCAGATCGAAGTTACCAAAACCAGAGAGTTTCACCTGTTCGCCGTTTTCCAGAGCGCGACGGATCTCTTCGAAAAACAGTTCAACCAGTTCTTTGGCATCCCGCTTGCTAAGCCCAAGCTTATCAAACAGATATTCTGACATTTCAGCTTTTGTAAGCGCCATAGGTTCAATTCCTCAATGATGCCTGGAATCGCTCTTTTAATGCCTCTACACATTTGGCGACGGTAGCGGCAAtct                                                                                                                                                                          >  2:144974/1‑251 (MQ=255)                                                     aTTCTTATCACGCAGATCGAAGTTACCAAAACCAGAGAGTTTCACCTGTTCGCCGTTTTCCAGAGCGCGACGGATCTCTTCGAAAAACAGTTCAACCAGTTCTTTGGCATCCCGCTTGCTAAGCCCAAGCTTATCAAACAGATATTCTGACATTTCAGCTTTTGTAAGCGCCATAGGTTCAATTCCTCAATGATGCCTGGAATCGCTCTTTTAATGCCTCTACACATTTGGCGACGGTAGCGGCAATctcc                                                                                                                                                                        >  2:114698/1‑251 (MQ=255)                                                                    aTCGAAGTTACCAAAACCAGAGAGTTTCACCTGTTCGCCGTTTTCCAGAGCGCGACGGATCTCTTCGAAAAACAGTTCAACCAGTTCTTTGGCATCCCGCTTGCTAAGCCCAAGCTTATCAAACAGATATTCTGACATTTCAGCTTTTGTAAGCGCCATAGGTTCAATTCCTCAATGATGCCTGGAATCGCTCTTTTAATGCCTCTACACATTTGGCGACGGTAGCg                                                                                                                                                                                 >  1:298303/1‑227 (MQ=255)                                                                    aTCGAAGTTACCAAAACCAGAGAGTTTCACCTGTTCGCCGTTTTCCAGAGCGCGACGGATCTCTTCGAAAAACAGTTCAACCAGTTCTTTGGCATCCCGCTTGCTAAGCCCAAGCTTATCAAACAGATATTCTGACATTTCAGCTTTTGTAAGCGCCATAGGTTCAATTCCTCAATGATGCCTGGAATCGCTCTTTTAATGCCTCTACACATTTGGCGACGGTAGCg                                                                                                                                                                                 <  2:298303/227‑1 (MQ=255)                                                                     tCGAAGTTACCAAAACCAGAGAGTTTCACCTGTTCGCCGTTTTCCAGAGCGCGACGGATCTCTTCGCAAAACAGTTCAACCAGTTCTTTGGCATCCCGCTTGCTAAGCCCAAGCTTATCAAACAGATATTCTGACATTTCAGCTTTTGTAAGCGCCATAGGTTCAATTCCTCAATGATGCCTGGAATCGCTCTTTTAATGCCTCTACACATTTGGCGACGGTAGCGGCAATCTCCTCTTCTTCGAGTGTAc                                                                                                                                                        >  1:53434/1‑251 (MQ=255)                                                                           ttACCAAAACCAGAGAGTTTCACCTGTTCGCCGTTTTCCAGAGCGCGACGGAACTCTTCGAAAAACAGTTCAACCAGTTCTTTGGCATCCCGCTTGCTAAGCCCAAGCTTATCAAACAGATATTCTGACATTTCAGCTTTTGTAAGCGCCATAGGTTCAATTCCTCAATGATGCCTGGAATCGCTCTTTTAATGCCTCTACACATTTGGCGACGGTAGCGGCAATCTCCTCTTCTTCGAGTGTACGGCTgg                                                                                                                                                  >  1:347927/1‑251 (MQ=255)                                                                             aCCAAAACCAGAGAGTTTCATCTGTTCGCCGTTTTCCAGAGCGCGACTGATCTCTTCGAAAAACAGTTCAACCAGTTCTTTGGCATCCCGCTTGCTAAGCCCAAGCTTATCAAACAGATATTCTGACATTTCAGCTTTTGTAAGCGCCATAGGTTCAATTCCTCAATGATGCCTGGAATCGCTCTTTTAATGCCTCTACACATTTGGCGACGGTAGCGGCAATCTCCTCTTCTTCGAGTGTACGGCTGGTa                                                                                                                                                <  1:2477/251‑1 (MQ=255)                                                                                 aaaCCAGAGAGTTTCACCTGTTCGCCGTTTTCCAGAGCGCGACGGATCTCTTCGAAAAACAGTTCAAACAGTTCTTTGGCATCCCGCTTGCTAAGCCCAAGCTTATCAAACAGATATTCTGACATTTCAGCTTTTGTAAGCGCCATAGGTTCAATTCCTCAATGATGCCTGGAATCGCTCTTTTAATGCCTCTACACATTTGGCGACGGTAGCGGCAATCTCCTCTTCTTCGAGTGTACGGCTGGTATCtt                                                                                                                                            >  1:393492/1‑251 (MQ=255)                                                                                      agagagTTTCACCTGTTCGCCGTTTTCCAGAGCGCGACGGATCTCTTCGAAAAACAGTTCAACCAGTTCTTTGGCATCCCGCTTGCTAAGCCCAAGCTTATCAAACAGATATTCTGACATTTCAGCTTTTGTAAGCGCCATAGGTTCAATTCCTCAATGATGCCTGGAATCGCTCTTTTAATGCCTCTACACATTTGGCGACGGTAGCGGCAATCTCCTCTTCTTCGAGTGTACGGCTGGTATCTTGCAgg                                                                                                                                       >  1:93646/1‑251 (MQ=255)                                                                                           gTTTCACCTGTTCGCCGTTTTCCAGAGCGCGACGGATCTCTTCGAAAAACAGTTCAACCAGTTCTTTGGCATCCCGCTTGCTAAGCCCAAGCTTATCAAACAGATATTCTGACATTTCAGCTTTTGTAAGCGCCATAGGTTCAATTCCTCAATGATGCCTGGAATCGCTCTTTTAATGCCTCTACACATTTGGCGACGGTAGCGGCAATCTCCTCTTCTTCGAGTGTACGGCTGGTATCTTGCAGGATCAg                                                                                                                                  <  1:376597/251‑1 (MQ=255)                                                                                                 ccTGTTCGCCGTTTTCCAGAGCGCGACGGATCTCTTCGAAAAACAGTTCAACCAGTTCTTTGGCATCCCGCTTGCTAAGCCCAAGCTTATCAAACAGATATTCTGACATTTCAGCTTTTGTAAGCGCCATAGGTTCAATTCCTCAATGATGCCTGGAATCGCTCTTTTAATGCCTCTACACATTTGGCGACGGTAGCGGCAATCTCCTCTTCTTCGAGTGTACGGCTGGTATCTTGCAGGATCAGGCTTAt                                                                                                                            <  1:144496/251‑1 (MQ=255)                                                                                                 ccTGTTCGCCGTTTTCCAGAGCGCGACGGATCTCTTCGAAAAACAGTTCAACCAGTTCTTTGGCATCCCGCTTGCTAAGCCCAAGCTTATCAAACAGATATTCTGACATTTCAGCTTTTGTAAGCGCCATAGGTTCAATTCCTCAATGATGCCTGGAATCGCTCTTTTAATGCCTCTACACATTTGGCGACGGTAGCGGCAATCTCCTCTTCTTCGAGTGTACGGCTGGTATCTTGCAGGATCAGGCTTAt                                                                                                                            >  1:103126/1‑251 (MQ=255)                                                                                                              ttCCAGAGCGCGACGGATCTCTTCGAAAAACAGTTCAACCAGTTCTTTGGCATCCCGCTTGCTAAGCCCAAGCTTATCAAACAGATATTCTGACATTTCAGCTTTTGTAAGCGCCATAGGTTCAATTCCTCAATGATGCCTGGAATCGCTCTTTTAATGCCTCTACACATTTGGCGACGGTAGCGGCAATCTCCTCTTCTTCGAGTGTACGGCTGGTATCTTGCAGGATCAGGCTTATGGCGAGGCTCtt                                                                                                                <  1:415262/250‑1 (MQ=255)                                                                                                               tCCAGAGCGAGACGGATCTCTTCGAAAAACAGTTCAACCAGTTCTTTGGCATCCCGCTTGCTAAGCCCAAGCTTATCAAACCGATATTCTGACATTTCAGCTTTTGTAAGCGCCATAGGTTCAATTCCTCAATGATGCCTGGAATCGCTCTTTTAATGCCTCTACACATTTGGCGACGGTAGCGGCAATCTCCTCTTCTTCGAGTGTACGGCTGGTATCTTGCAGGATCAGGCTTATGGCGCGGCTCTtat                                                                                                              >  1:172277/1‑251 (MQ=255)                                                                                                                                ctctTCGAAAAACAGTTCAACCAGTTCTTTGGCATCCCGCTTGCTAAGCCCAAGCTTATCAAACAGATATTCTGACATTTCAGCTTTTGTAAGCGCCATAGGTTCAATTCCTCAATGATGCCTGGAATCGCTCTTTTAATGCCTCTACACATTTGGCGACGGTAGCGGCAATCTCCTCTTCTTCGAGTGTACGGCTGGTATCTTGCAGGATCAGGCTTATGGCGAGGCTCTTATACCCCTCCGCAACAccc                                                                                             >  1:16392/1‑251 (MQ=255)                                                                                                                                       aaaaaCAGTTCAACCAGTTCTTTGGCATCCCGCTTGCTAAGCCCAAGCTTATCAAACAGATATTCTGACATTTCAGCTTTTGTAAGCGCCATAGGTTCAATTCCTCAATGATGCCTGGAATCGCTCTTTTAATGCCTCTACACATTTGGCGACGGTAGCGGCAATCTCCTCTTCTTCGAGTGTACGGCTGGTATCTTGCAGGATCAGGCTTATGGCGAGGCTCTTATACCCCTCCGCAACACCCTTACcgc                                                                                      >  1:288485/1‑251 (MQ=255)                                                                                                                                             aGTTCAACCAGTTCTTTGGCATCCCGCTTGCTAAGCCCAAGCTTATCAAACAGATATTCTGACATTTCAGCTTTTGTAAGCGCCATAGGTTCAATTCCTCAATGATGCCTGGAATCGCTCTTTTAATGCCTCTACACATTTGGCGACGGTAGCGGCAATCTCCTCTTCTTCGAGTGTACGGCTGGTATCTTGCAGGATCAGGCTTATGGCGAGGct                                                                                                                   >  2:344072/1‑216 (MQ=255)                                                                                                                                             aGTTCAACCAGTTCTTTGGCATCCCGCTTGCTAAGCCCAAGCTTATCAAACAGATATTCTGACATTTCAGCTTTTGTAAGCGCCATAGGTTCAATTCCTCAATGATGCCTGGAATCGCTCTTTTAATGCCTCTACACATTTGGCGACGGTAGCGGCAATCTCCGCTTCTTCGAGTGTACGGCTGGTATCTTGCAGGATCAGGCTTATGGCGAGGct                                                                                                                   <  1:344072/216‑1 (MQ=255)                                                                                                                                                 cAACCAGTGCTTTGGCATCCCGCTTGCTAAGCCCAAGCTTATCAAACAGATATTCTGACATTTCAGCTTTTGTAAGCGCCATAGGTTCAATTCCTCAATGATGCCTGGAATCGCTCTTTTAATGCCTCTACACATTTGGCGACGGTAGCGGCAATCTCCTCTTCTTCGAGTGTACGGCTGGTATCTTGCAGGATCAGGCTTATGGCGAGGCTCTTATACCCCTCCGCAACACCCTTACCTCGGTACACGTc                                                                            <  2:57847/251‑1 (MQ=255)                                                                                                                                                      aGTTCTTTGGCATCCCGCTTGCTAAGCCCAAGCTTATCAAACAGATATTCTGACATTTCAGCTTTTGTAAGCGCCATAGGTTCAATTCCTCAATGATGCCTGGAATCGCTCTTTTAATGCCTCTACACATTTGGCGACGGTAGCGGCAATCTCCTCTTCTTCGAGTGTACGGCTGGTATCTTGCAGGATCAGGCTTATGGCGAGGCTCTTATACCCCTCCGCAACACCCTTTCCGCGGTACACGTCAAATa                                                                       <  2:93646/251‑1 (MQ=255)                                                                                                                                                                              aGCCCAAGCTTATCAAACAGATATTCTGACATTTCAGCTTTTGTAAGCGCCATAGGTTCAATTCCTCAATGATGCCTGGAATCGCTCTTTTAATGCCTCTACACATTTGGCGACGGTAGCGGCAATCTCCTCTTCTGCGAGTGTACGGCTGGTATCTTGCAGGATCAGGCTTATGGCGAGGCTCTTATACCCCTCCGCAACACCCTTACCGCGGTACACGTCAAATAAGTTTACGCCAACTACCTGATTTa                                               <  2:322131/251‑1 (MQ=255)                                                                                                                                                                                         agcAAACAGATATACTGACATTTCAGCTTTTGTAAGCGCCATAGGTTCAATTCCTCAATGATGCCTGGAATCGCTCTTTTAATGCCTCTACACATTTGGCGACGGTAGCGGCAATCTCCTCTTCTTCGAGTGTACGGCTGGTATCTTGCAGGATCAGGCTTATGTCGAGGCTCTTATACCCCTCCGCAACACCCTTACCGCTGTACACGTCAAATAAGTTTACGCCAACTACCTGATTTACGCCAACTTTc                                    <  1:94202/249‑1 (MQ=255)                                                                                                                                                                                                                   cTTTTGTCAGCGCCATAGGTTCAATTCCTCAATGATGCCTGGTATCGATCTTTTAATGCCTCTACACATTTGGCGACGGTAGCGGCAATCTCCTCTTCTTCGAGTGTACGGCTGGAATCTTGCAGGATCAGGCTTATGGCGCGGCTCTTATACCCCTCCGCAACACCCTTACCGCGGTACACGTCAAATAAGTTTACGCCAACTACCTGATTTACGCCAACTTTCTTACATTCGGATAAAATATCCGCtac          >  2:251639/1‑249 (MQ=255)                                                                                                                                                                                                                      ttGTAAGCGCCATAGGTTCAATTCCTCAATGATGCCTGGAATCGCTCTTTTAATGCCTCTACACATTTGGCGACGGTAGCGGCAATCTCCTCTTCTTCGAGTGTACGGCTGGTATCTTGCAGGATCAGGCTTATGGCGAGGCTCTTATACCCCTCCGCAACACCCTTACCGCGGTACACGTCAAATAAGTTTACGCCAACTACCTGATTTACGCCAACTTTCTTACATTCGGATAAAATATCCGCTGCggg       >  1:324537/1‑251 (MQ=255)                                                                                                                                                                                                                      ttGTAAGCGCCATAGGTTCAATTCCTCAATGATGCCTGGAATCGCTCTTTTAATGCCTCTACACATTTGGCGACGGTAGCGGCAATCTCCTCTTCTTCGAGTGTACGGCTGGTATCTTGCAGGATCAGGCTTATGGCGAGGCTCTTATACCCCTCCGCAACACCCTTACCGCGGTACACGTCAAATAAGTTTACGCCAACTACCTGATTTACGCCAACTTTCTTACATTCGGATAAAATATCCGCTGCggg       >  2:484007/1‑251 (MQ=255)                                                                                                                                                                                                                           aGCGCCATAGGTTCAATTCCTCAATGATGCCTGGAATCGCTCTTTTAATGCCTCTACACATTTGGCGACGGTAGCGGCAATCTCCTCTTCTTCGAGTGTACGGCTGGTATCTTGCAGGATCAGGCTTATGGCGAGGCTCTTATACCCCTCCGCAACACCCTTACCGCGGTACACGTCAAATAAGTTTACGCCAACTACCTGATTTACGCCAACTTTCTTACATTCGGATAAAATATCCGCTGCGGGAACGt  >  1:200583/1‑251 (MQ=255)                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                          GTGCTGTAATGGGAATATCCTCGCCCGTTTTCGGGTTACGTCCCGGGCGTTGATTCTTATCACGCAGATCGAAGTTACCAAAACCAGAGAGTTTCACCTGTTCGCCGTTTTCCAGAGCGCGACGGATCTCTTCGAAAAACAGTTCAACCAGTTCTTTGGCATCCCGCTTGCTAAGCCCAAGCTTATCAAACAGATATTCTGACATTTCAGCTTTTGTAAGCGCCATAGGTTCAATCCCTCAATGATGCCTGGAATCGCTCTTTTAATGCCTCTACACATTTGGCGACGGTAGCGGCAATCTCCTCTTCTTCGAGTGTACGGCTGGTATCTTGCAGGATCAGGCTTATGGCGAGGCTCTTATACCCCTCCGCAACACCCTTACCGCGGTACACGTCAAATAAGTTTACGCCAACTACCTGATTTACGCCAACTTTCTTACATTCGGATAAAATATCCGCTGCGGGAACGT  >  NC_000913/1795327‑1795795

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑