Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,738,631	T→C	L263P (CTG→CCG)	purR →	DNA‑binding transcriptional repressor PurR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,738,631	0	T	C	100.0%	162.6 / NA	52	L263P (CTG→CCG)	purR	DNA‑binding transcriptional repressor PurR
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (22/30);  total (22/30)

CCCGGCCCGCTGGAACGTAACACCGGCGCAGGCCGCCTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCAGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACTGCCGTCTTCTGTGGTGGCGATATCATGGCAATGGGCGCACTTTGTGCTGCTGATGAAATGGGCCTGCGCGTCCCGCAGGATGTTTCGCTGATCGGTTATGATAACGTGCGCAACGCGCGCTATTTTACGCCGGCGCTGACCACGATCCATCAGCCAAAAGATTCGCTGGGTGAAACAGCGTTCAACATGCTGTTGGATCGTATCGTCAACAAACGTGAAGAACCGCAGTCTATTGAAGTGCATCCGCGCTTGATTGAACGCCGCTCCGTGGCTGACGGCCCGTTCCGCGACTATCGTCGTTAATCACCCGT  >  NC_000913/1738396‑1738877                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                       cccGGCCCGCTGGAACGTAACACCGGCGCAGGCCGCCTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCAGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACTGCCGTCTTCTGTGGTGGCGATATCATGGCAATGGGCGCACTTTGTGCTGCTGATGAAATGGGCCCGCGCGGCCCGCAggg                                                                                                                                                                                                                                         >  2:125782/1‑250 (MQ=255)                 gTAACACCGGCGCAGGCCGCCTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCAGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACTGCCGTCTTCTGTGGTGGCGATATCATGGCAATGGGCGCACTTTGTGCTGCTGATGAAATGGGCCCGCGCGTCCCGCAGGATGTTTCGCTGATCGGt                                                                                                                                                                                                                         >  1:381329/1‑251 (MQ=255)                      acCGGCGCAGGCCGCCTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCAGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACTGCCGTCTTCTGTGGTGGCGATATCATGGCAATGGGCGCACTTTGTGCTGCTGATGAAATGGGCCCGCGCGTCCCGCAGGATGTTTCGCTGATCGGTTATGa                                                                                                                                                                                                                    <  1:187322/251‑1 (MQ=255)                      acCGGCGCAGGCCGCCTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGATGGCTTGTGCAGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACTGCCGTCTTCTGTGGTGGCGATATCATGGCAATGGGCGCACTTTGTGCTGCTGATGAAATGGGCCCGCGCGTCCCGCAGGATGTTTCGCTGATCGGTTATg                                                                                                                                                                                                                     >  1:352409/1‑250 (MQ=255)                         ggCGCAGGCCGACGTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGGGCCGGAAAGCTGGATTGTGCAGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGACGCATCGCCCTACTGCCGTCTTCTGTGGTGGCGATATCATGGCAATGGGCGCACTTTGTGCTGCTGATGAAATGGGCCCGCGCGTCCCGCAGGATGTTTCGCTGATCGGTTATGATaa                                                                                                                                                                                                                 <  2:478953/251‑1 (MQ=255)                           cgcAGGCCGCCTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCAGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACTGCCGTCTTCTGTGGTGGCGATATCATGGCAATGGGCGCACTTTGTGCTGCTGATGAAATGGGCCCGCGCGTCCCGCAGGATGTTTCGCTGATCGGTTATGATAACg                                                                                                                                                                                                               <  1:96088/251‑1 (MQ=255)                            gcAGGCCGCCTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCAGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACTGCCGTCTTCTGTGGTGGCGATATCATGGCAATGGGCGCTCTTTGTGCTGCTGATGAAATGGTCCCGCGCGTCCCGCAGGATGTTTTGCTTATCGGTTATGATAACGt                                                                                                                                                                                                              <  2:35772/251‑1 (MQ=255)                                   gccTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCAGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACTGCCGTCTTCTGTGGTGGCGATATCATGGCAATGGGCGCACTTTGTGCTGCTGATGAAATGGGCCCGCGCGTCCCGCAGGATGTTTCGCTGATCGGTTATGATAACGTGCGCAAc                                                                                                                                                                                                       >  1:487268/1‑251 (MQ=255)                                    ccTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCAGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACTGCCGTCTTCTGTGGTGGCGATATCATGGCAATGGGCGCACTTTGTGCTGCTGATGAAATGGGCCCGCGCGTCCCGCAGGATGTTTCGCTGATCGGTTATGATAACGTGCGCAAcg                                                                                                                                                                                                      >  1:172091/1‑251 (MQ=255)                                           ggTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCAGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACTGCCGTCTTCTGTGGTGGCGATATCATGGCAATGGGCGCACTTTGTGCTGCTGATGAAATGGGCCCGCGCGTCCCGCAGGCTGTTTCGCTGATCGGTTATGATAACGTGCGCAACGCGCGCt                                                                                                                                                                                                >  1:80460/1‑250 (MQ=255)                                                tATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGACAGCTGGATTGTGCCGGGAGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACTGCCGTCTTCTGTGGTGGCGATATCATGGCAATGGGCGCACTTTGTGCTGCTGATGAAATGGGCCCGCGCGTCCCGCAGGATGTTTCGCTGATCGGTTATGATAACGTGCGCAACGCGCGCTAtttt                                                                                                                                                                                           >  2:295425/1‑250 (MQ=255)                                                     aGGCGAGGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCAGGGTGACTGTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACTGCCGTCTTCTGTGGTGGCGATATCATGGCAATGGGCGCACTTTGTGCTGCTGATGAAATGGGCCCGCGCGTCCCGCAGGATGTTTCGCTGATCGGTTATGATAACGTGCGCAACGCGCGCTATTTTACGCCg                                                                                                                                                                                     <  2:155598/251‑1 (MQ=255)                                                         gATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCAGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACTGCCGTCTTCTGTGGTGGCGATATCATGGCAATGGGCGCACTTTGTGCTGCTGATGAAATGGGCCCGCGCGTCCCGCAGGATGTTTCGCTGATCGGTTATGATAACGTGCGCAACGCGCGCTATTTTACGCCGgcgc                                                                                                                                                                                 >  1:280302/1‑251 (MQ=255)                                                          aTGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCAGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACTGCCGTCTTCTGTGGTGGCGATATCATGGCAATGGGCGCACTTTGTGCTGCTGATGAAATGGGCCCGCGCGTCCCGCAGGATGTTTCGCTGATCGGTTATGATAACGTGCGCAACGCGCGCTATTTTACGCCGGCGCt                                                                                                                                                                                <  2:148423/251‑1 (MQ=255)                                                            ggAAGAAGCACTGATCAAGGTGCCGGAAAGCTGGATTGTGCAGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACTGCCGTCTTCTGTGGTGGAGATATCATGGCAATGGGCGCACTTTGTGCTGCTGATGAAATGGGCCCGCGCGTCCCGCAGGATGTTTAGCTGATCGGTTATGATAACGTGCGCAACGCGCGCTATTTTACGACGGCGCTg                                                                                                                                                                               >  2:551182/1‑250 (MQ=255)                                                              aagaagCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCAGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACTGCCGTCTTCTGTGGTGGCGATATCATGGCAATGGGCGCACTTTGTGCTGCTGATGAAATGGGCCCGCGCGTCCCGCAGGATGTTTCGCTGATCGGTTATGATAACGTGCGCAACGCGCGCTATTTTACGCCGGCGCTGAcc                                                                                                                                                                            >  1:3183/1‑251 (MQ=255)                                                                    cGATGATCAAGGTGCCGGAAAGCGGGAGTGTGCAGGGTGACGTTGACCCTGAAGCCGGTTATCGCGCCATGCAGCAAATCCTGGCGCAGCCGCATCGCCCTACTGCCGTCTTCTGTGGTGGCGATAGCATGGCAATGGGCGCACTTTGTGCGGCTGATGAAATGGGCCCGCGCGGCCCGCAGGATGTGTCGCTGATCGGGTATGAGAACGTGCGGAACGCGCGCTATTGTACGCCGGCGCTGACCACGATc                                                                                                                                                                      <  2:352409/251‑1 (MQ=255)                                                                      atgatCAAGGTGCCGGAAAGCTGGATTGTGCAGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACTGCCGTCTTCTGTGGTGGCGATATCATGGCAATGGGCGCACTTTGTGCTGCTGATGAAATGGGCCCGCGCGTCCCGCAGGATGTTTCGCTGATCGGTTATGATAACGTGCGCAACGCGCGCTATTTTACGCCGGCGCTGACCACGATCCa                                                                                                                                                                    <  1:69768/251‑1 (MQ=255)                                                                                   cGGAAAGCTGGATTGTGCAGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACTGCCGTCTTCTGTGGTGGCGATATCATGGCAATGGGCGCACTTTGTGCTGCTGATGAAATGGGCCCGCGCGTCCCGCAGGATGTTTCGCTGATCGGTTATGATAACGTGCGCAACGCGCGCTATTTTACGCCGGCGCTGACCACGATCCATCAGCCaaa                                                                                                                                                           >  1:561376/1‑247 (MQ=255)                                                                                     gAAAGCTGGATTGTGCAGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACTGCCGTCTTCTGTGGTGGCGATATCATGGCAATGGGCGCACTTTGTGCTGCTGATGAAATGGGCCCGCGCGTCCCGCAGGATGTTTCGCTGATCGGTTATGATAACGTGCGCAACGCGCGCTATTTTACGCCGGCGCTGACCACGATCCATCAGCCAAAAGagtc                                                                                                                                                     <  2:108071/251‑4 (MQ=255)                                                                                             gATTGTGCAGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACTGCCGTCTTCTGTGGTGGCGATATCATGGCAATGGGCGCACTTTGTGCTGCTGATGAAATGGGCCCGCGCGTCCCGCAGGATGTTTCGCTGATCGGTTATGATAACGTGCGCAACGCGCGCTATTTTACGCCGGCGCTGACCACGATCCATCAGCCAAAAGATTCGCTGGGTg                                                                                                                                             <  1:61147/251‑1 (MQ=255)                                                                                                      gggTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACTGCCGTCTTCTGTGGTGGCGATATCATGGCAATGGGCGCACTTTGTGCTGCTGATGAAATGGGCCCGCGCGTCCCGCAGGATGTTTCGCTGATCGGTTATGATAACGTGCGCAACGCGCGCTATTTTACGCCGGCGCTGACCACGATCCATCAGCCAAAAGATTCGCTGGGTGAAACAGCGt                                                                                                                                    <  2:425730/251‑1 (MQ=255)                                                                                                          gACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACTGCCGTCTTCTGTGGTGGCGATATCATGGCAATGGGCGCACTTTGTGCTGCTGATGAAATGGGCCCGCGCGTCCCGCAGGATGTTTCGCTGATCGGTTATGATAACGTGCGCAACGCGCGCTATTTTACGCCGGCGCTGACCACGATCCATCAGCCAAAAGATTCGCTGGGTGAAACAGCGTTCa                                                                                                                                 >  1:540917/1‑250 (MQ=255)                                                                                                               tGAACCTGAATCCTTTTATCGCGCCATGCAGCAAATCCTGTCTCAGCCGCATCGCCCTACTGCCGTCTTCTGTGGTTGCGATATCATGGCAATGGGCTCATTTTGTGCTGCTGATGAAATGGGCCCGCGCTTCCCGCAGGATGTTTCGCTGATCGGTTGTGATATCTTGCGCAACGCGCGCTATTTGACGTCGGCGCTGACCACGTTCCATCATCCAAAAGATTCGCTGTGTTAAACAGCGTTCAACATg                                                                                                                            <  2:349341/250‑1 (MQ=255)                                                                                                               tGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACTGCCGTCTTCTGTGGTGGCGATATCATGGCAATGGGCGCACTTTGTGCTGCTGATGAAATGGGCCCGCGCGTCCCGCAGGATGTTTCGCTGATCGGTTATGATAACGTGCGCAACGCGCGCTATTTTACGCCGGCGCTGACCACGATCCATCAGCCAAAAGATTCGCTGGGTGAAACAGCGTTCAACATg                                                                                                                            <  1:132881/250‑1 (MQ=255)                                                                                                                gAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACTGCCGTCTTCTGTGGTGGCGATATCATGGCAATGGGCGCACTTTGTGCTGCTGATGAAATGGGCCCGCGCGTCCCGCAGGATGTTTCGCTGATCGGTTATGATAACGTGCGCAACGCGCGCTATTTTACGCCGGCGCTGACCACGATCCATCAGCCAAAAGATTCGCTGGGTGAAACAGCGTTCAACATGCt                                                                                                                          <  1:137987/251‑1 (MQ=255)                                                                                                                            ggTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACTGCCGTCTTCTGTGGTGGCGATATCATGGCAATCGGCGCACTTTGTGCGGCTGATGAAATGGACCCGCGCGTCCCGCAGGTTGGGTCGCTGATCGGTTATGATACCGTGCGCAACGCGCGCTATTTTACGCCAGCGCTGACCACGATCCATCAGCCAAAAGATTCGCTGGGTGAAACAGCGTTCAACATGCTGTTGGatcgtat                                                                                                              >  1:308028/1‑251 (MQ=255)                                                                                                                               tATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACTGCCGTCTTCTGTGGTGGCGATATCATGGCAATGGGCGCACTTTGTGCTGCTGATGAAATGGGCCCGCGCGTCCCGCAGGATGTTTCGCTGATCGGTTATGATAACGTGCGCAACGCGCGCTATTTTACGCCGGCGCTGACCACGATCCATCAGCCAAAAGATTCGCTGGGTGAAACAGCGTTCAACATGCTGTTGGatcgtatcg                                                                                                            <  1:563236/250‑1 (MQ=255)                                                                                                                                aTCGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACTGCCGTCTTCTGTGGTGGCGATATCATGGCAATGGGCGCACTTTGTGCTGCTGATGAAATGGGCCCGCGCGTCCCGCAGGATGTTTCGCTGATCGGTTATGATAACGTGCGCCACGCGCGCTATTTTACGCCGGCGCTGACCACGATCCATCAGCCAAAAGATTCGCTGGGTGAAACCGCGTTCAACATGCTGTTGGATCGTATCGTc                                                                                                          >  1:265341/1‑251 (MQ=255)                                                                                                                                aTCGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACTGCCGTCTTCTGTGGTGGCGATATCATGGCAATGGGCGCACTTTGTGCTGCTGATGAAATGGGCCCGCGCGTCCCGCAGGATGTTTCGCTGATCGGTTATGATAACGTGCGCAACGCGCGCTATTTTACGCCGGCGCTGACCACGATCCATCAGCCAAAAGATTCGCTGGGTGAAACAGCGTTCAACATGCTGTTGGATCGTATCGTc                                                                                                          >  2:201027/1‑251 (MQ=255)                                                                                                                                    cgcCATGCAGCAACTCCAGTCGCAGCGGCATTGCCCTACTGCCGTCTTCTGTGGTGGCGATATCATGGCACTGGGCGCACTTTGTACTGCTGATGAACTGGGCCCGCGCGTCCCGCAGGATGTTTCGCTGATCGGTTATGATAACGTGCGCAACGAGCGCTATTTTACGCCGGCGCGGACCACGATCCATCAGCCAAAAGATTCGCTGGGTGCAACAGCGTTCAACATGCTGTTGGATCGTATCGTcaac                                                                                                       >  2:535873/1‑250 (MQ=255)                                                                                                                                         tGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACTGCCGTCTTCTGTGGTGGCGATATCATGGCAATGGGCGCACTTTGTGCTGCTGATGAAATGGGCCCGCGCGTCCCGCAGGATGTTTCGCTGATCGGTTATGATAACGTGCGCAACGCGCGCTATTTTACGCCGGCGCTGACCACGATCCATCAGCCAAAAGATTCGCTGGGTGAAACAGCGTTCAACATGCTGTTGGATCGTATCGTCAACAAACGt                                                                                                 >  1:241254/1‑251 (MQ=255)                                                                                                                                          gctgcaaatccgtacgcaacggCATCGCCCGACTGCCGTCTTCGGAGGTGGGGATATCATGGCAATGGGCGCACTTTGTGCTGCAGATGAAATGGGCCCGCGCGTCCCGCATGATGTTTGGCTTATCGGTTATGAAAACGTGCGCAACGCGCGCTATTGTACGCGGTCGCGGACCACGATCCAAGAGGGAAAATAGTCGCTGAGAGAAACAGCGTTCAACATGCTGATGGGACGTATCGTCAACAAACGt                                                                                                 <  2:522529/229‑1 (MQ=255)                                                                                                                                          gcagcaAATCCTGTCGCAGCCGCATCGCCCTACTGCCGTCTTCTGTGGTGGCGATATCATGGCAATGGGCGCACTTTGTGCTGCTGATGAAATGGGCCCGCGCGTCCCGCAGGATGTTTCGCTGATCGGTTATGATAACGTGCGCAACGCGCGCTATTTTACGCCGGCGCTGACCACGATCCATCAGCCAAAAGATTCGCTGGGTGAAACAGCGTTCAACATGCTGTTGGATCGTATCGTCAACAAACGt                                                                                                 <  1:131451/250‑1 (MQ=255)                                                                                                                                               aaagccTGTCGCAGCCGCATCGCCCTACTGCCGTCTTCTGTGGTGGCGATATCATGGCAATGGGCGCACTTTGTGCTGCTGATGAAATGGGCCCGCGCGTCCCGCAGGATGTTTCGCTGATCGGTTATGATAACGTGCGCAACGCGCGCTATTTTACGCCGGCGCTGACCACGATCCATCAGCCAAAAGATTCGCTGGGTGAAACAGCGTTCAACATGCTGTTGGATCGTATCGTCAACAAACGTgaagaa                                                                                           <  2:347503/247‑1 (MQ=255)                                                                                                                                                     tGTCGCAGCCGCATCGCCCTACTGCCGTCTTCTGTGGTGGCGATATCATGGCAATGGGCGCACTTTGTGCTGCTGATGAAATGGCCCCGCGCGTCCCGCAGGATGTTTCGCTGATCGGTTATGATAACGTGCGCAACGCGCGCTATTTTACGCCGGCGCTGACCACGCTCCATCAGCCAAAAGATTCGCTGGGTGAAACAGCGTTCAACATGCTGTTGGATCGTATCGTCAACAAACGTGAAGAACCGc                                                                                       >  1:336637/1‑249 (MQ=255)                                                                                                                                                         gCAGCCGCACCGCCCTACTGCCGTCTTCTGTGGTGGCGATATCATGGCAATGGGCGCACTTTGTGCTGCTGATGAAATGGGCCCGCGCGTCCCGCAGGATGTTTCGCTGATCGGTTATGATAACGTGCGCAACGCGCGCTATTTTACGCCGGCGCTGACCACGATCCATCAGCCAAAAGATTCGCTGGGTGAAACAGCGTTCAACATGCTGTTGGATCGTATCGTCAACAAACGTGAAGACCCGCAGTCTa                                                                                 <  2:48842/251‑1 (MQ=255)                                                                                                                                                          cAGCCGCATCGCCCTACTGCCGTCTTCTGTGGAGGCGATATCATGGCAATGGGCGCACTTTGTGCTGCTGATGAAATGGGCCCGCGCGTCCCGCAGGATGTTTCGCTGATCGGTTATGATAACGTGCGCAACGCGCGCTATTTTACGCCGGCGCTGACCACGATCCATCAGCCAAAAGATTCGCTGGGTGCAACAGCGTTCAACATGCTGTTGGATCGTATCGTCAACAAACGTGAAGAACCGCAGTCTAt                                                                                >  1:402077/1‑251 (MQ=255)                                                                                                                                                          cAGCCGCATAGACCTACTGCCGTCTACTGTGGTGGCGATATCCTGGCCATGGGCGCACTTTGTGCTGCTGATGAAATGGGCCCGCGCGTCCCGCAGGATGTTTCGCTGATCGGTTATGATAACGTGCGCAACGCGCGCTATTTTACGCCGGCGCTGACCACGATCCATCAGCCAACAGATTCGCTGGGTGAAACAGCGTTCAACATGCTGTTGGATCGTATAGTCCACAAACGCGAAGAACCGCCGTCTAt                                                                                >  2:78333/1‑251 (MQ=255)                                                                                                                                                              cGCATCGCCCTACTGCCGTCTTCTGTGGTGGCGATATCATGGCAATGGGCGCACTTTGTGCTGCTGATGAAATGGGCCCGCGCGTCCCGCAGGATGTTTCGCTGATCGGTTATGATAACGTGCGCAACGCGCGCTATTTTACGCCGGCGCTGACCACGATCCATCAGCCAAAAGATGCGCTGGGTGAAACAGCGTTCAACATGCTGTTGGATCGTATCGTCAACAAACGTGAAGAACCGCAGTCTATTGaa                                                                            <  2:410931/251‑1 (MQ=255)                                                                                                                                                                 aTCGCCCTACTGCCGTCTTCTGTGGTGGCGATATCATGGCAATGGGCGCACTTTGTGCTGCTGATGAAATGGGCCCGCGCGTCCCGCAGGATGTTTCGCTGATCGGTTATGATAACGTGCGCAACGCGCGCTATTTTACGCCGGCGCTGACCACGATCCATCAGCCAAAAGATTCGCTGGGTGAAACAGCGTTCAACATGCTGTTGGATCGTATCGGCAACAAACGTGAAGAACCGCAGTCTATTGAAGTg                                                                         <  2:241254/251‑1 (MQ=255)                                                                                                                                                                            gCCGTCTTCTGTGGTGGCGATATCATGGCAATGGGCGCACTTTGTGCTGCTGATGAAATGGGCCCGCGCGTCCCGCAGGATGTTTCGCTGATCGGTTATGATAACGTGCGCAACGCGCGCTATTTTACGCCGGCGCTGACCACGATCCATCAGCCAAAAGATTCGCTGGGTGAAACAGCGTTCAACATGCTGTTGGATCGTATCGTCAACAAACGTGAAGAACCGCAGTCTATTGAAGTGCATCCGCGCtt                                                              >  1:445723/1‑251 (MQ=255)                                                                                                                                                                                      gtggggGCGATATCATGGCAATGGGCGCACTTTGTGCTGCTGATGAAATGGGCCCGCGCGTCCCGCAGGATGTTTCGCTGATCGGTTATGATAACGTGCGCAACGCGCGCTATTTTACGCCGGCGCTGACCACGATCCATCAGCCAAAAGATTCGCTGGGTGAAACAGCGTTCAACATGCTGTTGGATCGTATCGTCAACAAACGTGAAGAACCGCAGTCTATTGAAGTGCATCCGCGCTTGATTGaa                                                       <  2:172091/248‑1 (MQ=255)                                                                                                                                                                                            gCGATATCATGGCAATGGGCGCACTTTGTGCTGCTGATGAAATGGGCCCGCGCGTCCCGCAGGATGTTTCGCGGATCGGTTATGATAACGTGCGCAACGCGCGCTATTTTACGCCGGCGCTGACCACGATCCATCAGCCAAAAGATTCGCTGGGTGAAACAGCGTTCAACATGCTGTTGGAGCGTATCGTCAACAAACGTGAAGAACCGCAGTCTATTGAAGTGCATCCGCGCTTGATTGAACGCCGCTcc                                              <  2:544439/251‑1 (MQ=255)                                                                                                                                                                                              gATATCATGGCAATGGGCGCACGTTGTGCTGCTGATGAAATGGGCCCGCGCGTCCCGCAGGATGTTTCGCTGATCGGTTATGATAACGTGCGCAACGCGCGCTAGTTTACGCCGGCGCTGACCACGATCCATCAGCCAAAAGATTCGCTGGGTGAAACAGCGTTCAACATGCTGGTGGATCGTATCGTCAACAAACGTGAAGAACCGCAGTCTATTGAAGTGCATCCGCGCTTGATTGAACGCCGCTCCGt                                            <  1:555286/251‑1 (MQ=255)                                                                                                                                                                                                            gggCGCACTTTGTGCTGCTGATGAAATGGGCCCGCGCGTCCCGCAGGATGTTTCGCTGATCGGTTATGATAACGTGCGCAACGCGCGCTATTTTACGCCGGCGCTGACCACGATCCATCAGCCAAAAGATTCGCTGGGTGAAACAGCGTTCAACATGCTGTTGGATCGTATCGTCAACAAACGTGAAGAACCGCAGTCTATTGAAGTGCATCCGCGCTTGATTGAACGCCGCTCCGTGGCTGACGGCCCg                               <  1:582069/250‑1 (MQ=255)                                                                                                                                                                                                               cgcACTTTGTGCTGCTGATGAAATGGGCCCGCGCGTCCCGCAGGATGTTTCGCTGATCGGTTATGATAACGTGCGCAACGCGCGCTATTTTACGCCGGCGCTGACCACGATCCATCAGCCAAAAGAGTCGCTGGGTGAAACAGCGTTCAACATGCTGTTGGATCGTATCGTCAACAAACGTGAAGAACCGCAGTCTATTGAAGTGCATCCGCGCTTGATTGAACGCCGCTCCGTGGCTGACGGCCCGTTcc                           <  2:584670/251‑1 (MQ=255)                                                                                                                                                                                                                 cACTTTGTGCTGATGATGAAATGTGCCCGCGCGTCCCGCAGGATGTTTCGCTGATCGGTTATGATAACGTGCGCAACGCGCGCTATTTTACGCCGGCGCTGACCACGATCCATCAGCCAAAAGATTCGCTGGGTGAAACAGCGTTCAACATGCTGTTGGATCGTATCGTCAACAAACGTGAAGAACCGCAGTCTATTGAAGTGCATCCGCGCTTGATTGAACGCCGCTCCGTGGAGGACGGCCCGTTCcgc                         <  2:136415/251‑1 (MQ=255)                                                                                                                                                                                                                            gctgATGAAATGGGCCCGCGCGTCCCGCAGGATGTTTCGCTGATCGGTTATGATAACGTGCGCAACGCGCGCTATTTTACGCCGGCGCTGACCACGATCCATCAGCCAAAAGATTCGCTGGGTGAAACAGCGTTCAACATGCTGTTGGATCGTATCGTCAACAAACGTGAAGAACCGCAGTCTATTGAAGTGCATCCGCGCTTGATTGAACGCCGCTCCGTGGCTGACGGCCCGTTCCGCGACTAtcgtc               <  1:201014/250‑1 (MQ=255)                                                                                                                                                                                                                                    aaTGGGCCCGCGCGTCCCGCAGGATGTTTCGCTGATCGGTTATGATAACGTGCGCAACGCGCGCTAGTTTACGCCGGCGCTGACCACGATCCATCAGCCAAAAGATTCGCTGGGTGAAACAGCGTTCAACATGCTGTTGGATCGTATCGTCAACAAACGTGAAGAACCGCAGTCTATTGAAGTGCATCCGCGCTTGATTGAACGCCGCTCCGTGGCTGACGGCCCGTTCCGCGACTAGCGTCGTTAATCAc      <  1:106040/251‑1 (MQ=255)                                                                                                                                                                                                                                        ggCCCGCGCGTCCCGCAGGATGTTTCGCTGATCGGTTATGATAACGTGCGCAACGCGCGCTATTTTACGCCGGCGCTGACCACGATCCATCAGCCAAAAGATTCGCTGGGTGAAACAGCGTTCAACATGCTGTTGGATCGTATCGTCAACAAACGTGAAGAACCGCAGTCTATTGAAGTGCATCCGCGCTTGATTGAACGCCGCTCCGTGGCTGACGGCCCGTTCCGCGACTATCGTCGTTAATCACCCGt  >  1:493754/1‑251 (MQ=255)                                                                                                                                                                                                                                        ggCCCGCGCGTCCCGCAGGATGTTTCGCTGATCGGTTATGATAACGTGCGCAACGCGCGCTATTTTACGCCGGCGCTGACCACGATCCATCAGCCAAAAGATTCGCTGGGTGAAACAGCGTTCAACATGCTGGTGGATCGTATCGTCAACAAACGTGAAGAACCGCAGTCTATTGAAGTGCATCCGCGCTTGATTGAACGCCGCTCCGTGGCTGACGGCCCGTTCCGCGACTATCGCCGTTAATCACCCGt  <  2:82342/251‑1 (MQ=255)                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                       CCCGGCCCGCTGGAACGTAACACCGGCGCAGGCCGCCTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCAGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACTGCCGTCTTCTGTGGTGGCGATATCATGGCAATGGGCGCACTTTGTGCTGCTGATGAAATGGGCCTGCGCGTCCCGCAGGATGTTTCGCTGATCGGTTATGATAACGTGCGCAACGCGCGCTATTTTACGCCGGCGCTGACCACGATCCATCAGCCAAAAGATTCGCTGGGTGAAACAGCGTTCAACATGCTGTTGGATCGTATCGTCAACAAACGTGAAGAACCGCAGTCTATTGAAGTGCATCCGCGCTTGATTGAACGCCGCTCCGTGGCTGACGGCCCGTTCCGCGACTATCGTCGTTAATCACCCGT  >  NC_000913/1738396‑1738877

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑