Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,534,321	C→T	P100S (CCA→TCA)	nhoA →	arylamine N‑acetyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,534,321	0	C	T	100.0%	100.6 / NA	33	P100S (CCA→TCA)	nhoA	arylamine N‑acetyltransferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (12/21);  total (12/21)

ATACGCTTCGTGCATTGCACCTGAAACACAATTGCACCATTCCGTTTGAAAACCTCGACGTTTTGCTGCCGAGGGAAATACAGCTTGATAATCAATCGCCGGAAGAGAAACTGGTGATAGCCCGTCGTGGCGGTTACTGTTTTGAGCAGAATGGCGTGTTTGAGCGGGTGTTACGCGAGCTGGG‑GTTTAACGTTCGCAGCTTGTTAGGGCGCGTAGTGTTATCAAATCCGCCAGCATTACCGCCGCGCACCCATCGTTTGCTGTTGGTGGAACTGGAAGAGGAAAAATGGATTGCTGATGTCGGTTTCGGTGGGCAGACGCTAACCGCGCCGATTCGTTTAGTTTCCGATCTCGTGCAGACCACGCCACACGGAGAGTATCGGTTGTTGCAGGAGGGTGATGATTGGGTGTTGCAGTTTAATCATCATCAGCATTGGCAGTCGATGTACCGTTTTGATCTCTGCG  >  NC_000913/1534091‑1534555                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                           aTACGCTTCGTGCATTGCACTTGAAACACAATTGCACCATTCCGTTTGAAAACCTCGACGTTTTGCTGCCGAGGGAAATACAGCTTGATAATCAATCGCCGGAAGAGAAACTGGTGATAGCCCGTCGTGGCGGTTACTGTTTTGAGCAGAATGGCGTGTTTGAGCGGGTGTTACGCGAGCTGGG‑GTTTAACGTTCGCAGCTTGTTAGGGCGCGTAGTGTTATCAAATCCGTCAGCATTACCGCCGCGCAcc                                                                                                                                                                                                                        <  1:388131/251‑1 (MQ=255) aTACGCTTCGTGCATTGCACCTGAAACACAAATGCACCATTCCGTTTGAAAACCTCGACGTTTTGCTGCCGAGGGAAATACAGCTTGATAATCAATCGCCGGAAGAGAAACTGGTGATAGCCCGTCGTGGCGGTTACTGTTTTGAGCAGAATGGCGTGTTTGAGCGGGTGTTACGCGAGCTGGG‑GTTTAACGTTCGCAGCTTGTTAGGGCGCGTAGTGTTATCAAATCCGTCAGCATTACCGCCGCGCAcc                                                                                                                                                                                                                        <  2:145730/251‑1 (MQ=255)   aCGCTTCGTGCATTGCACCTGAAACACAATTGCACCATTCCGTTTGAAAACCTCGACGTTTTGCTGCCGAGGGAAATACAGCTTGATAATCAATCGCCGGAAGAGAAACTGGTGATAGCCCGTCGTGGCGGTTACTGTTTTGAGCAGAATGGCGTGTTTGAGCGGGTGTGACGCTAGCTGGG‑TTTGAACGGTCGCAGCTTTGTAGGTCGCGTAGTGTTATCAAATCCGTCAGCATTACCTCCGCGCAccc                                                                                                                                                                                                                       <  2:305196/250‑1 (MQ=255)                                                    cccgcGACGTTTTGCTGCCGAGGGAAATACAGCTTGATAATCAATCGCCGGAAGAGAAACGGGTGATAGCCCGTCGTGGCGGTTACTGTTTTGAGCAGAATGGCGTGTTTGAGCGTGTGTTACGCGAGCTGGG‑GTTTAACGTTCGCAGCTTGTTAGGGCGCGTAGTGTTATCAAATCCGTCAGCATTACCGCCGCGCACCCATCGTTTTCTGTTGGTGGAACTGGAAGAGGAAAAATGGATTGCTGATGTc                                                                                                                                                                     <  2:385348/247‑1 (MQ=255)                                                     ccTCGACGTTTTGCTGCCGAGGGAAATACAGCTTGATAATCAATCGCCGGAATAGAAACTGGTGATAGCCCGTCGTGGCGGTTACTGTTTTGAGCAGAATGGCGTGTTTGAGCGGGTGTTACGCGAGCTGGG‑GTTTAACGTTCGCAGCTTGTTAGGGCGCGTAGTGTTATCAAATCCGTCAGCATTACCGCCGCGCACCCATCGTTTGCTGTTGGTGGAACTGGAAGAGGAAAAATGGATTGCTGATGTCg                                                                                                                                                                    <  1:275099/251‑1 (MQ=255)                                                                 gctgcCGAGGGAAATACAGCTTGATAATCAATCGCCGGAAGAGAAACTGGTGATAGCCCGTCGTGGCGGTTACTGTTTTGAGCAGAATGGCGTGTTTGAGCGGGTGTTACGCGAGCTGGG‑GTTTAACGTTCGCAGCTTGTTAGGGCGCGTAGTGTTATCAAATCCGTCAGCATTACCGCCGCGCACCCATCGTTTGCTGTTGGTGGAACTGGAAGAGGAAAAATGGATTGCTGATGTCGGTTTCGGTggg                                                                                                                                                         <  1:239833/250‑1 (MQ=255)                                                                 gctgcCGAGGGAAATACAGCTTGATAATCAAGCGCCGGAAGAGAAACTGGTGATAGCCCGTCGTGGCGGTTACTGTGTTGAGCAGAATGGCGTGTTTGAGCGGGTGTTACGCGAGCTGGG‑GTTTAACGTTCGCAGCTTGTTAGGGCGCGTAGTGGTAGCAAATCCGTCAGCATTACCGCCGCGCACCCAGCGTTTGCTGTTGGTGGAACTGGAAGAGGAAAAATGGATTGCTGATGTCGGTTTCGGTGGGc                                                                                                                                                        <  2:48985/251‑1 (MQ=255)                                                                            aaaTACAGCTTGATAATCAATCGTCGGAAGAGAAACTGGGGATGGCCCGGCGTGGCGTTTTCTGTTTTGAGCAGAAGGGCGTGTGTGAGCGGGTGTTACGCGAGCTGGG‑GTTTAACGGTCGCAGCTTGCTAGGGCGCGTAGGGTTATCAATGCCGTCAGCATTACCGCAGCGCACCCATCGTGTGCTGTTGGTGGAACTGGAAGAGGAAAAATGGATTGCTGAAGTCGGTTTCGGTGGTCAGACCCTAAcc                                                                                                                                             <  2:413568/251‑1 (MQ=255)                                                                             aaTACAGCTTGATAATCAATCGGCGGAAGAGAAACGGGTGATAGCCCGTCGTGGCGGTTACTGTTTTGAGCAGAATGGCGTGTTTGAGCGGGTGTTACGCGAGCTGGG‑GTTTAACGTTCGCAGCTTGTTAGGGCGCGTAGTGTTATCAAATCCGTCAGCATTACCGCCGCGCACCCATCGTTTGCTGTTGGTGGAACTGGAAGAGGAAAAATGGATTGCTGATGTCGGTTTCGGTGGGCAGACGCTAACcg                                                                                                                                            <  2:5428/251‑1 (MQ=255)                                                                             aaTACAGCTTGATAATCAATCGCCGGAAGAGAATCTGGTGATAGCCCGTCGTGTCGGTTACTGTTTTGAGCAGAATGGCGTGTTTGAGCGGGTGTTACGCGAGCTGGG‑GTTTAACGTTCGCAGCTTGTTAGGGCGCGTAGTGTTATCAAATCCGTCAGCATTACCGCCGCGCACCCATCGTTTGCTGTTGGTGGAACTGGAAGAGGAAAAATGGATTGCTGATGTCGGTTTCGGTGGGCAGACGCTAACcg                                                                                                                                            <  2:352471/251‑1 (MQ=255)                                                                                      tGATAATCAATCGCCGGAAGAGAAACTGGTGATAGCCCGTCGTGGCGGTTACTGTTTTGAGCAGAATGGCGTGTTTGAGCGGGTGTTACGCGAGCTGGG‑GTTTAACGTTCGCAGCTTGTTAGGGCGCGTAGTGTTATCAAATCCGTCAGCATTACCGCCGCGCACCCAGCGTTTGCTGTTGGTGGAACTGGAAGAGGAAAAATGGATTGCTGATGTCGGTTTCGGTGGGCAGACGCTAACCGCGCCGAtt                                                                                                                                    <  1:260373/250‑1 (MQ=255)                                                                                       taTAATCAATCGCCGGAAGAGAAACTGGTGATAGCCCGTCGTGGCGGTTATTGTTTTGAGCAGAATGGCGTGTTTGAGCGGGTGTTACGCGAGCTGGG‑GTTTAACGTTCGCAGCTTGTTAGGGCGCGTAGTGTTATGAAATCCGTCAGCATTACCGCCGCGCTCCCATCGTTTGCTGTTTGTGGAACTGGAAGAGCAAAAATGGATTTCTGATGTCGGTTTCTGTGGGCAGACGCTAACAGCGCCGATTc                                                                                                                                   <  2:389780/249‑1 (MQ=255)                                                                                                        agagAAACTGGTGATAGCCCGTCGTGGCGGTTACTGTTTTGAGCAGAATGGCGTGTTTGAGCGGGTGTTACGCGAGCTGGG‑GTTTAACGTTCGCAGCTTGTTAGGGCGCGTAGTGTTATCAAATCCGTCAGCATTACCGCCGCGCACCCATCGTTTGCTGTTGGTGGAACTGGAAGAGGAAAAATGGATTGCTGATGTCGGTTTCGGTGGGCAGACGCTAACCGCGCCGATTCGTTTAGTTTCCGATCTCg                                                                                                                 <  2:124948/251‑1 (MQ=255)                                                                                                        agagAAACTGGTGATAGCCCGTCGTGGCGGTTACTGTTTTGAGCAGAATGGCGTGTTTGAGCGGGTGTTACGCGAGCTGGG‑GTTTAACGTTCGCAGCTTGTTAGGGCGCGTAGTGTTATCAAATCCGTCAGCATTACCGCCGCGCACCCATCGTTTGCTGTTGGTGGAACTGGAAGAGGAAAAATGGATTGCTGATGTCGGTTTCGGTGGGCAGACGCTAACCGCGCCGATTCGTTTAGTTTCCGATCTCg                                                                                                                 <  2:316494/251‑1 (MQ=255)                                                                                                                          ccGTCGTGTCGGTTACTTTGTGGAGCATAATGGCGTGTTTGAGCGGGTGTTACGCGAG‑TGGGTGTTTAACGTTGGCAGCTTGTTAGGGCGCGTAGTGTGATCATAGCCGTCAGCATTACCGCCGCGCACCCATCGTTTGCTGTTGGTGTAACTGTAAGAGGAAAAATTTATTGAGGATGTCGGTTGCGGTGGGTAGACGCTAACCGCGCCGATTCGTTTATTTTCTAATCTCGGGCAGTCCAGGCCTCACg                                                                                               <  2:550398/251‑1 (MQ=255)                                                                                                                                      ttACTGTTTTGAGCAGAATGGCGTGTTTGAGCGGGTGTTACGCGAGCTGGG‑GTTTAACGTTCGCAGCTTGTTAGGGCGCGTAGTGTTATCAAATCCGTCAGCATTACCGCCGCGCACCCATCGTTTGCTGTTGGTGGAACTGGAAGAGGAAAAATGGATTGCTGATGTCGGTTTCGGTGGGCAGACGCTAACCGCGCCGATTCGTTTAGTTTCCGATCTCGTGCAGACCACGCCACACGGAGAGTATCGgt                                                                                   >  1:261308/1‑251 (MQ=255)                                                                                                                                        aCTGTTTTGAGCAGAATGGCGTGTTTGAGCGGGTGTTACGCGAGCTGGG‑GTTTAACGTTCGCAGCTTGTTAGGGCGCGTAGTGTTATCAAATCCGTCAGCATTACCGCCGCGCACCCATCGTTTGCTGTTGGTGGAACTGGAAGAGGAAAAATGGATTGCTg                                                                                                                                                                          >  1:515710/1‑162 (MQ=255)                                                                                                                                        aCTGTTTTGAGCAGAATGGCGTGTTTGAGCGGGTGTTACGCGAGCTGGG‑GTTTAACGGTCGCAGCTTGTTAGGGCGCGTAGTGTTATCAAATCCGTCAGCATTACCGCCGCGCACCCATCGTTTGCTGTTGGTGGAACTGGAAGAGGAAAAATGGATTGCTg                                                                                                                                                                          <  2:515710/162‑1 (MQ=255)                                                                                                                                        aCTGTTTTGAGCAGAATGGCGTGTTTGAGCGGGCGTTACGCGCGCTGGG‑GTTTAACGTTCGCAGCTTGTTAGGGCGCGTAGTGTTATCAAATCCGTCAGCATTACCGCCGCGCACCCATCGTTTGCTGTTGGTGGAACTGGAAGAGGAAAAATGGCTTGCTGATGTCGGTTTCGGTGGGCAGACGCTAACCGCGCCGATTCGTTTAGTTTCCGATCTCGTGCCGACCCCGCCACACGGAGGGTCTCGgttg                                                                                 >  2:19977/1‑251 (MQ=255)                                                                                                                                            ttttGAACAGAATGGCTTTTTTCAGCGGGTGGTCAGTGAGCTTGG‑GTTTAACGTTCGCAGCTTGGTAGGGCGCATAGTGTTATCACATCCGTCAGCATTACCGCCGCGCACCCCTCGTTTGCTGTTGGTGGACCTGGAAGAGGAAAAATGGATTGCTGATGTCGTTTTCGGTGGGCAGCCGCTAACCGCTACGGTTCGTTTAGTTTCCGATCTCGTGCAGACCACCCCACCCGGTGAGTATCGGTTGATGc                                                                             >  2:397457/1‑251 (MQ=255)                                                                                                                                                   cAGAATGGCGTGTTTGAGCGGGTGTTACGCGAGCTGGG‑GTTTAACGTTCGCAGCTTGTTAGGGCGCGTAGTGTTATCAAATCCGTCAGCATTACCGCCGCGCACCCATCGTTTGCTGTTGGTGGAACTGGAAGAGGAAAAATGGATTGCTGATGTCGGTTTCGGTGGGCAGACGCTAACCGCGCCGATTCGTTTAGTTTCCGATCTCGTGCAGACCACGCCACACGGAGAGTATCGGTTGTTGCAGGAggg                                                                      >  1:523212/1‑251 (MQ=255)                                                                                                                                                     gAATGGCGTGTTTGAGCGGGTGTTACGCGAGCTGGG‑GTTTAACGTTCGCAGCTTGTTAGGGCGCGTAGTGTTATCAAATCCGTCAGCATTACCGCCGCGCACCCATCGTTTGCTGTTGGTGGAACTGGAAGAGGAAAAATGGATTGCTGATGTCGGTTTCGGTGGGCAGACGCTAACCGCGCCGATTCGTTTAGTTTCCGATCTCTTGCAGACCACGCCACACGGAGAGTATCGGTTGTTGCAGGAGGGtg                                                                    <  1:389231/251‑1 (MQ=255)                                                                                                                                                         ggCGTGTTTGAGCGGGTGTTACGCGAGCTGGG‑GTTTAACGTTCGCAGCTTGTTGGGGCGCGTAGTGTTAGCAAATCCGTCAGCATTACCGCCGCGCACCCATCGTTTGCTGTTGGTGGAACTGGAAGAGGAAAAATGGATTGCTGATGTCGGTTGCGGTGGGCAGACGCTAACCGCGCCGATTCGTTTAGTTTCCGATCTCGTGCAGATCACGCCACACGGAGAGTATCGGTTGTTGCAGGAGGGtgatga                                                                <  2:450801/251‑1 (MQ=255)                                                                                                                                                              gtTTGAGCGGGTGTTACGCGAGCTGGG‑GTTTAACGTTCGCAGCTTGTTAGGGCGCGTAGTGTTATCAAATCCGTCAGCATTACCGCCGCGCACCCATCGTTTGCTGTTGGTGGAACTGGAAGAGGAAAAATGGATTGCTGATGTCGGTTTCg                                                                                                                                                              >  1:57696/1‑152 (MQ=255)                                                                                                                                                              gtTTGAGCGGGTGTTACGCGAGCTGGG‑GTTTAACGTTCGCAGCTTGTTAGGGCGCGTAGTGTTATCAAATCCGTCAGCATTACCGCCGCGCACCCATCGTTTGCTGTTGGTGGAACTGGAAGAGGAAAAATGGATTGCTGATGTCGGTTTCg                                                                                                                                                              <  2:57696/152‑1 (MQ=255)                                                                                                                                                                      gggtgtaacaagAGCGGGG‑GTTTAAAGTTCGCAGCTTGTTAGGGCGCGTAGTGTTATCAAATCAGTCAGCATTAACGCCACGCACCCATCGGTTGCTGTTGGTGGAACTGGAAGAGGAAAAATGGATTGCTGAGGTCGGTTTCGCTGGGCCGACGATAACCGCGCCGCTTCGTTTGGTTTCTGACCTCGTGCAGACCACGCCACCCGGAGAGAAGCGGTTGTTGCAGGAGGGTGCTGAGTGGGTGTTGccg                                                   >  2:231363/12‑249 (MQ=255)                                                                                                                                                                         tgtTACGCGAGCTGGG‑GTTTAACGTTCGCAGCTTGTTAGGGCGCGTAGTGTTATCAAATCCGTCAGCATTACCGCCGCGCACCCATCGTTTGCTGTTGGTGGAACTGGAAGAGGAAAAATGGATTGCTGATGTCGGTTTCGGTGGGCAGACGCTAACCGCGCCGATTCGTTCAGTTTCCGATCTAGTGCAGACCACGCCACACGGAGAGTATCGGATGTTGCAGGAGGGTGATGATTGGGTGTTGCAGttt                                                >  1:50781/1‑251 (MQ=255)                                                                                                                                                                            tACGCGAGCTGGG‑GTTTAACGTTCGCAGCTTGTTAGGGCGCGTAGTGTTATCAAATCCGTCAGCATTACCGCCGCGCACCCATCGTTTGCTGTTGGTGGAACTGGAAGAGGAAAAATGGATTGCTGATGTCGGTTTCGGTGGGCAGACGCTAACCGCGCCGATTCGTTTAGTTTCCGATCTCGTGCAGACCACGCCACACGGAGAGTATCGGTTGTTGCAGGAGGGTGATGATTGGGTGTTGCAGTTTAat                                             >  1:131394/1‑251 (MQ=255)                                                                                                                                                                               gcgAGCTGGG‑GTTTAACGTTCGCAGCTTGTTAGGGCGCGTAGTGTTATCAAATCCGTCAGCATTACCGCCGCGCACCCATCGTTTGCTGTTGGTGGAACTGGAAGAGGAAAAATGGATTGCTGATGTCGGTTTCGGTGGGCAGACGCTAACCGCGCCGATTCGTTTAGTTTCCGATCTCGTGCAGACCACGCCACACGGAGAGTATCGGTTGTTGCAGGAGGGTGATGATTGGGTGTTGCAGTTTAatcat                                          <  1:277652/251‑1 (MQ=255)                                                                                                                                                                               gcgAGCTGGG‑GTTTAAAGTTCGCAGCTTGTTAGGGCGCGTAGTGTTATCAAATCCGTCAGCATTACCGCCGCGCACCCATCGTTTGCTGTTGGTGGAACTGGAAGAGGAAAAATGGATTGCTGATGTCGGTTTCGGTGGGCAGACGCTAACCGCGCCGATTCGTTTAGTTTCCGATCTCGTGCAGACCACGCCACACGGAGAGTATCGGTTGTTGCAGGAGGGTGATGATTGGGTGTTGCAGTTTAatcat                                          >  1:435978/1‑251 (MQ=255)                                                                                                                                                                                                               ggggCGCGTAGTGTTATCAAATCCGTCAGCATTACCGCCGCGCACCCATCGTTTGCTGTTGGTGGAACTGGAAGAGGAAAAATGGATTGCTGATGTCGGTTTCGGTGGGCAGACGCTAACCGCGCCGATTCGTTTAGTTTCCGATCTCGTGCAGACCACGCCACACGGAGAGTATCGGTTGTTGCAGGAGGGTGATGATTGGGTGTTGCAGTTTAATCATCAGCAGCATTGGCAGTCGATGTACCGTTTTg           <  2:287726/250‑1 (MQ=255)                                                                                                                                                                                                                 ggCGCGTAGTGTTATCAAATCCGTCAGCATTACCGCCGCGCACCCATCGTTTGCTGTTGGTGGAACTGGAAGAGGAAAAATGGCTTGCTGATGTCGGTTTCGGTGGGCAGACGCTAACCGCGCCGATTCGTTTAGTTTCCGATCTCGTGCAGACCACGCCACACGGAGAGTATCGGTTGTTGCAGGAGGGGGATGATTGGGTGTTGCAGTTTAATCATCATCAGCATTGGCAGTCGATGGACCGTTTTGAt         >  2:393844/1‑251 (MQ=255)                                                                                                                                                                                                                          tgtTATCAAATCCGTCAGCATTACCGCCGCGCACCCATCGTTTGCTGTTGGTGGAACTGGAAGAGGAAAAATGGATTGCTGATGTCGGTTTCGGTGGGCAGACGCTAACCGCGCCGATTCGTTTCGTTTCCGATCTCGTGCAGACCACGCCACACGGAGAGTATCGGTTGTTGCAGGAGGGTGATGATTGGGTGTTGCAGTTTAATCATCATCAGCCTTGGCAGTCGATGTACCGTTTTGATCTCTGCg  >  1:301172/1‑249 (MQ=255)                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                           ATACGCTTCGTGCATTGCACCTGAAACACAATTGCACCATTCCGTTTGAAAACCTCGACGTTTTGCTGCCGAGGGAAATACAGCTTGATAATCAATCGCCGGAAGAGAAACTGGTGATAGCCCGTCGTGGCGGTTACTGTTTTGAGCAGAATGGCGTGTTTGAGCGGGTGTTACGCGAGCTGGG‑GTTTAACGTTCGCAGCTTGTTAGGGCGCGTAGTGTTATCAAATCCGCCAGCATTACCGCCGCGCACCCATCGTTTGCTGTTGGTGGAACTGGAAGAGGAAAAATGGATTGCTGATGTCGGTTTCGGTGGGCAGACGCTAACCGCGCCGATTCGTTTAGTTTCCGATCTCGTGCAGACCACGCCACACGGAGAGTATCGGTTGTTGCAGGAGGGTGATGATTGGGTGTTGCAGTTTAATCATCATCAGCATTGGCAGTCGATGTACCGTTTTGATCTCTGCG  >  NC_000913/1534091‑1534555

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑