Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,531,318	C→T	intergenic (‑814/‑498)	yncO ← / → ydcC	protein YncO/H repeat‑associated putative transposase YdcC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,531,318	0	C	T	100.0%	51.9 / NA	18	intergenic (‑814/‑498)	yncO/ydcC	protein YncO/H repeat‑associated putative transposase YdcC
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (8/10);  total (8/10)

AACCATTGATGCTATGGGCTGTCAGAAAGATATCGCTTCGAAGATCAAAGATAAAAAAGCAGATTATCTTCTGGCAGTAAAAGGCAATCAGGGGAAATTACATCATGCATTCGAGGAAAAATTTCCTGTAAATGTGTTTTCTAATTATAAAGGCGATTCGTTTAGTACGCAGGAGATAAGTCATGGAAGAAAAGAAACACGTTTGCATATTGTCAGTAACGTAACGCCTGAACTTTTGTGATTTTGAATTCGAATGGAAGGGATTAAAAAAGCTTTGTGTAGCATTGTCATTCAGGCAGAAGAAAGAAGATAAATCAGCAGAAGGTGTAAGCATCCGATATTATATTTCATCAAAGGATATGGATGCTAAAGAATTTGCACATGCTATCAGAGCGCACTGGCTGATCGAGCACAGTCTTCATTGGGTGTTAGATGTAAAAATGAATGAAGATGCCAGCCGGATA  >  NC_000913/1531092‑1531555                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                              aaCCATTGATGCTATGGGCTGTCAGAAAGATATCGCTTCGAAGATCAAAGATAAAAAAGCAGATTATCTTCTGGCAGTAAAAGGCAATCAGGGGAAATTACATCATGCATTCGAGGAAAAATTTCCTGTAAATGTGTTTTCTAATTATAAAGGCGATTCGTTTAGTACGCAGGAGATAAGTCATGGAAGAAAAGAAACACGTTTGCATATTGTCAGTAACGTAACGTCTGAACTTTTGTGATTTTGAATTc                                                                                                                                                                                                                       <  1:344655/251‑1 (MQ=255)                     gTCAGAAAGATATCGCTTCGAAGATCAAAGATAAAAAAGCAGATTATCTTCTGGCAGTAAAAGGCAATCAGGGGAAATTACATCATGCATTCGAGGAAAAATTTCCTGTAAATGTGTTTTCTAATTATAAAGGCGATTCGTTTAGTACGCAGGAGATAAGTCATGGAAGAAAAGAAACACGTTTGCATATTGTCAGTAACGTAACGTCTGAACTTTTGTGATTTTGAATTCGAATGGAAGGGATTaaaa                                                                                                                                                                                                     <  2:161994/249‑1 (MQ=255)                                     ttCGAAGATCAAAGATAAAAAAGCAGATTATCTTCTGGCAGTAAAAGGCAATCAGGGGAAATTACATCATGCATTCGAGGAAAAATTTCCTGTAAATGTGTTTTCTAATTATAAAGGCGATTCGTTTAGTACGCAGGAGATAAGTCATGGAAGAAAAGAAACACGTTTGCATATTGTCAGTAACGTAACGTCTGAACTTTTGTGATTTTGAATTCGAATGGAAGGGATTAAAAAAGCTTTGTGTAGCATTg                                                                                                                                                                                   >  1:577833/1‑251 (MQ=255)                                           gATCAAAGATAAAAAAGCAGATTATCTTCTGGCAGTAAAAGGCAATCAGGGGAAATGACATCATGCATTCGAGGAAAAATTTCCTGTAAATGTGTTTTCTAATTATAAAGGCGATTCGTTTAGTACGCAGGAGATAAGTCATGGAAGAAAATAAACACGTTTGCATATTGTCAGTAACGTAACGTCTGAACTTTTGTGAGTTTGAATTCGTATGGAAGGGATTAAAAAAGCTTTGTGTAGCATTGGCATTc                                                                                                                                                                             <  2:208665/251‑1 (MQ=255)                                              cAAAGATAAAAAAGCAGATTATCTTCTGGCAGTAAAAGGCAATCAGGGGAAATTACATCATGCATTCGAGGAAAAATTTCCTGTAAATGTGTTTTCTAATTATAAAGGCGATTCGTTTAGTACGCAGGAGATAAGTCATGGAAGAAAAGAAACACGTTTGCATATTGTCAGTAACGTAACGTCTGAACTTTTGTGATTTTGAATTCGAATGGAAGGGATTAAAAAAGCTTTGTTTAGCATTGTCATTCAgg                                                                                                                                                                          >  2:245002/1‑251 (MQ=255)                                                   aTAAAAAAGCAGATTATCTTCTGGCAGTAAAAGGCAATCAGGGGATATTACATAATGCATGCGAGGAAAAAGTTCCTGTAAATGTGGTTGCTAATTATAAAGGCGATTCGTGTAGTACTCAGGAGATAAGGCATGGAAGAAAAGAAACACGTTTGCATATTGTCAGTAACGTAACTTCTGAACTTTTGTGATTTTGAATTCGAATGGAAGGGATTAAAAAAGATTTTTGTAGCATTGTTATGCAGGCagaa                                                                                                                                                                     <  2:489172/251‑1 (MQ=255)                                                             aGATTATCTTCTGGCAGTAAAAGGCAATCAGGGGAAATTACATCATGCATTCGAGGAAAAATTTCCTGTAAATGTGTTTTCTAATTATAAAGGCGATTCGTTTAGTACGCAGGAGATAAGTCATGGAAGAAAAGAAACACGTTTGCATATTGTCAGTAACGTAACGTCTGAACTTTTGTGATTTTGAATTCGAATGGAAGGGATTAAAAAAGCTTTGTGTAGCATTGTCATTCAGGCAGaagaaaga                                                                                                                                                               >  1:385920/1‑247 (MQ=255)                                                                         ggCAGTAAAAGGCAATCAGGGGAAATTACATCATGCATTCGAGGAAAAATTTCCTGTAAATGTGTTTTCTAATTATAAAGGCGATTCGTTTAGTTCGCAGGAGATAAGTCATGGAAGAAAAGAAACACGTTTGCATATTGTCAGTAACGTAACGTCTGAACTGTTGTGATTTTGAATTCGAATGGAAGGGATTAAAAAAGCTTTGTGTAGCATTGTCATTCAGGCAGAAGAAAGAAGATAAATcagcag                                                                                                                                                 <  1:563678/249‑1 (MQ=255)                                                                                                            cATTCGAGGAAAAATTTCCTGTAAATGTGTTTTCTAATTATAAAGGCGATTCGTTTAGTACGCAGGAGATAAGTCATGGAAGAAAAGAAACACGTTTGCATATTGTCAGTAACGTAACGTCTGAACTTTTGTGATTTTGAATTCGAATGGAAGGGATTAAAAAAGCTTTGTGTAGCATTGTCATTCAGGCAGAAGAAAGAAGATAAATCAGCAGAAGGTGTAAGCATCCGATATTATATTTCATCAAAGGa                                                                                                            <  1:141864/251‑1 (MQ=255)                                                                                                                                gTAAATGTGTTTTCTAATTATAAAGGCGATTCGTTTAGTACGCAGGAGATAAGTCATGGAAGAAAAGAAACACGTTTGCATATTGTCAGTAACGTAACGTCTGAACTTTTGTGATTTTGAATTCGAATGGAAGGGATTAAAAAAGCTTTGTGTAGCATTGTCATTCAGGCAGAAGAAAGAAGATAAATCAGCAGAATGTGTAAGCATCCGATATTATATTTCATCAAAGGATATGGATGCTAAAGAAtt                                                                                          <  1:375426/249‑1 (MQ=255)                                                                                                                                                     aaaGGCGAGTCGTTTAGTACGCAGGAGATAAGTCATGGAAGAAAAGAAACACGTTTGCATATTGTCAGTAACGTAACGTCTGAACTTTTGTGATTTTGAATTCGAATGGAAGGGATTAAAAAAGCTTTGTGTAGCATTGTCATTCAGGCAGAAGAAAGAAGATAAATCAGCAGAAGGTGTAAGCATCCGATATTATATTTCATCAAAGGATATGGATGCTAAAGAATTTGCACATGCTATCAGAGCGCAc                                                                    <  2:391917/250‑1 (MQ=255)                                                                                                                                                        ggCGATTCGTTTAGTCCGCAGGAGATAAGTCATGGACGAAAAGAAACACGTTTGCATATTGTCAGTAACGTAACGTCTGAACTTTTGTGATTTTGAATTCGAATGGAAGGGATTAAAAAAGCTTTGTGTAGCATTGTCATTCAGGCAGAAGAAAGAAGATAAATCAGCAGAAGGTGTAAGCATCCGATATTATATTTCATCAAAGGATATGGATGCTAACGAATTTGCACATGCTATCTGAGAGCACTGGc                                                                >  2:87804/1‑251 (MQ=255)                                                                                                                                                                                   aGTCATGGAAGAAAAGAAACACGTTTGCATATTGTCAGTAACGTAACGTCTGAACTTTTGTGATTTTGAATTCGAATGGAAGGGATTAAAAAAGCTTTGTGTAGCATTGTCATTCAGGCAGAAGAAAGAAGATAAATCAGCAGAAGgt                                                                                                                                            <  2:164692/148‑1 (MQ=255)                                                                                                                                                                                   aGTCATGGAAGAAAAGAAACACGTTTGCATATTGTCAGTAACGTAACGTCTGAACTTTTGTGATTTTGAATTCGAATGGAAGGGATTAAAAAAGCTTTGTGTAGCATTGTCATTCAGGCAGAAGAAAGAAGATAAATCAGCAGAAGgt                                                                                                                                            >  1:164692/1‑148 (MQ=255)                                                                                                                                                                                      cATGGAAGAAAAGAAACACGTTTGCATATTGTCAGTAACGTAACGTCTGAACTTTTGTGATTTTGAATTCGAATGGAAGGGATTAAAAAAGCTTTGTGTAGCATTGTCATTCAGGCAGAAGAAAGAAGATAAATCAGCAGAAGGTGTAAGCATCCGATATTATATTTCATCAAAGGATATGGATGCTAAAGAATTTGCACATGCTATCAGAGCGCACTGGCTGATCGAGCACAGTCTTCATTGGGTGtt                                    >  1:32238/1‑249 (MQ=255)                                                                                                                                                                                                           ttGCATATTGTCAGTAACGTAACGTCTGACCTTTTGTGATTTTGAATTCGAATGGAAGGGATTAAAAAAGCTTTGTGTAGCATTGTCATTCAGGCAGAAGAAAGAAGATAAATCAGCAGAAGGTGTAAGCATCCGATATTATATTTCATCAAAGGATATGGATGCTAAAGAATTTGCAAATGCTATCAGAGCACACTGGCTGATCGAACACAGTCTTCATTGGGAGTGAGATGTAAACATGAATGAAGATg             >  1:465521/1‑251 (MQ=255)                                                                                                                                                                                                                  ttGTCAGTAACGTAACGTCTGAACTTTTGTGATTTTGAATTCGAATGGAAGGGATTAAAAAAGCTTTGTGTAGCATTGTCATTCAGGCAGAAGAAAGAAGATAAATCAGCAGAAGGTGTAAGCATCCGATATTATATTTCATCAAAGGATATGGATGCTAAAGAATTTGCACATGCTATCAGAGCGCACTGGCTGATCGAGCACAGTCTTAATTGGGTGTTAGATGTAAAAATGAATGAAGCTGCAAGCCg      >  1:317093/1‑251 (MQ=255)                                                                                                                                                                                                                      cAGTAACGTAACGTATTATCTTTTGTGATTTTGAATTCGAATGGAAGGGATTAAAAAAGCTTTGTGTAGCAATTTCATTCAGGCAGAAGAAAGAAGATAAATCAGCAGAAGGTGTAAGCATCCGATATTATATTTCATCAAAGGATATGGATGCTAATGAATTTGCACATGCTATCAGAGCGCACTGGCTGATCTAGCACAGTCTTCACTGGGTGTTAGATGTAAAAATGAATGAAGATGCCAGCCGGATa  <  2:379422/251‑1 (MQ=255)                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                              AACCATTGATGCTATGGGCTGTCAGAAAGATATCGCTTCGAAGATCAAAGATAAAAAAGCAGATTATCTTCTGGCAGTAAAAGGCAATCAGGGGAAATTACATCATGCATTCGAGGAAAAATTTCCTGTAAATGTGTTTTCTAATTATAAAGGCGATTCGTTTAGTACGCAGGAGATAAGTCATGGAAGAAAAGAAACACGTTTGCATATTGTCAGTAACGTAACGCCTGAACTTTTGTGATTTTGAATTCGAATGGAAGGGATTAAAAAAGCTTTGTGTAGCATTGTCATTCAGGCAGAAGAAAGAAGATAAATCAGCAGAAGGTGTAAGCATCCGATATTATATTTCATCAAAGGATATGGATGCTAAAGAATTTGCACATGCTATCAGAGCGCACTGGCTGATCGAGCACAGTCTTCATTGGGTGTTAGATGTAAAAATGAATGAAGATGCCAGCCGGATA  >  NC_000913/1531092‑1531555

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑