| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,491,105 | C→T | T68T (ACC→ACT) | cybB → | superoxide oxidase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,491,105 | 0 | C | T | 96.5% | 65.7 / ‑6.6 | 29 | T68T (ACC→ACT) | cybB | superoxide oxidase |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (8/20); minor base G (1/0); total (9/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.10e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.61e-01 | |||||||||||
GCCCGTTATGTTAACGGAAAATTATGTTAAAGCAGGAAATGTTATGGAAAATAAATATTCAAGGTTACAAATCAGCATTCACTGGCTGGTCTTTTTACTGGTTATCGCAGCGTATTGCGCAATGGAGTTTCGTGGTTTCTTCCCACGTAGCGATCGGCCACTCATCAACATGATTCATGTTTCCTGTGGCATCTCAATTCTCGTGCTGATGGTCGTTCGTCTTTTGTTAAGGCTGAAATACCCAACCCCGCCGATTATACCTAAGCCAAAACCGATGATGACGGGACTGGCGCATTTGGGACATTTGGTGATTTATCTCCTGTTTATTGCGCTGCCAGTGATTGGTTTGGTGATGATGTATAACCGGGGCAACCCGTGGTTTGCGTTTGGTTTGACGATGCCTTACGCTTCAGAGGCCAATTTCGAACGGGTAGATAGCTTAAAGTCGTGGCATGAAACGCTGGCGAATCTGGGATATTTT > NC_000913/1490859‑1491339 | gCCCGTTATGTTAACGGAAAATTATGTTAAAGCAGGAAATGTTATGGAAAATAAATATTCAAGGTTACAAATCAGCATTCACTGGCTGGTCGTTTTACTGGTTATCGCAGCGTATTGCGCAATGGAGTTTCGTGGTTTCTTCCCACGTAGCGATCGGCCACTCATCAACATGATTCATGTTTCCTGTGGCATCTCAATTCTCGTGCTGATGGTCGTTCGTCTTTTGTTAAGGCTGAAATACCCAACTccgc < 1:417843/251‑1 (MQ=255) aGGAAATGTTATGGAAAATAAATATTCAAGGTTACAAATCAGCATTCACTGGCTGGTCTTTTTACTGGTTATCGCAGCGTATTGCGCAATGGAGTTTCGTGGTTTCTTCCCACGTAGCGATCGGCCACTCATCAACATGATTCATGTTTCCTGTGGCATCTCAATTCTCGTGCTGATGGTCGTTCGTCTTTTGTTAAGGCTGAAATACCCAACTCCGCCGATTATACCTAAGCCAAAACCGATGATGACgg < 1:382002/251‑1 (MQ=255) aattttATGGCAAACAAATATTCCAGGCTACAAATCAGCCTTCACTGGCTGGAATTTTTTCTGCTTATCGCAGAGAATCTAGCAATGGAGTTTCGTGGTTTATTCCAACGTAGCGATCGGCCACTCATCAACATGAATAATGTTTCCTGTGGCATCTCAAGTATCGTGCTGATGGTCGTTCGTCTTTTGTTAAGGCTGAAACACCCAAAGCCGCCGATTATACCTAAGCAaaaaactgtgatgaccggcat > 2:396490/5‑234 (MQ=255) aaataaatATTCAAGGTTACAAATCAGCATTCACTGGCTGGGCTTTTGACTGGTTATCGCAGCGTATGGCGCAATGGAGTTTCGTGGTTTCTTCCCACGTAGCGATCGGCCTCTCATCAACATGATGCATGTTTCCTGTGGCATCTCAATTCTCGTGCTGATTGTCGTTCGTCTTTTGTTAAGGCTGAAATACCCAACTCCGCCGATTATACCTAAGCCAAAGCCTACGATGACTGGACTGGCGCATTTgg < 2:376528/251‑1 (MQ=255) aaataTTCAAGGTTACAAATCAGCATTCACTGGCTGGTCTTTTTACTGGTTATCGCAGCGTATTGCGCAATGGAGTTTCGTGGTTTCTTCCCACGTAGCGATCGGCCACTCATCAACATGATTCATGTTTCCTGTGGCATCTCAATTCTCGTGCTGATGGTCGTTCGTCTTTTGTTAAGGCTGAAATACCCAACTCCGCCGATTATACCTAAGCCAAAACCGATGATGACGGGACTGGCGCATTTGGGACa < 2:379781/251‑1 (MQ=255) tCAAGGTTACAAATCAGCATTCACTGGCTGGTCTTTTTACTGGTTATCGCAGCGTATTGCGCAATGGAGTTTCGTGGTTTCTTCCCACGTAGCGATCGGCCACTCATCAACATGATTCATGTTTCCTGTGGCATCTCAATTCTCGTGCTGATGGTCGTTCGTCTTTTGTTAAGGCTGAAATACCCAACTCCGCCGATTATACCTAAGCCAAAACCGATGATGACGGGACTGGCGCATTTGGGACATTTgg < 1:262195/250‑1 (MQ=255) ggTTACAAATCAGCATTCACTGGCTGGTCTTTTTACTGGTTATCGCAACGTATTGCGCAATGGAGTTTCGTGGTTTCTTCCCACGTAGAGATCGGCCACTCATCAACATGATTCATGTTTCCTGTGGCATCTCAATTCTCGTGCTGATGGTCGTTCGTCTTTTGTTAAGGCTGAAATACCCAACTCCGCCGATTATACCTAAGCCAAACCCGATGATGACGGGACTGGCGCATTTGGGAACTTTGGTGAtt > 1:332087/1‑251 (MQ=255) tggTCTTTTTACTGGTTATCGCAGCGTATTGCGCAATGGAGTTTCGTGGTTTCTTCCCACGTAGCGATCGGCCACTCATCAACATGATTCATGTTTCCTGTGGCATCTCAATTCTCGTGCTGATGGTCGTTCGTCTTTTGTTAAGGCTGAAATACCCAACTCCGCCGATTATACCTAAGCCAAAACCGATGATGACGGGACTGGCGCATTTGGGACATTTGGTGATTTATCTCCTGTTTATTGCGCTGcc > 1:288573/1‑250 (MQ=255) ggTCTTTTTACTGGTTATCGCAGCGTATTGCGCAATGGAGTTTCGTGGTTTCTTCCCACGTAGCGATCGGCCACTCATCAACATGATTCATGTTTCCTGTGGCATCTCAATTCTCGTGCTGATGGTCGTTCGTCTTTTGTTAAGGCTGAAATACCCAACTCCGCCGATTATACCTAAGCCAAAACCGATGATGACGGGACTGGCGCATTTGGGACATTTGGTGATTTATCTCCTGTTTATTGCGCTGCCAg < 2:288573/251‑1 (MQ=255) ttttACTGGTTATCGCAGCGTATTGCGCAATGGAGTTTCGTGGTTTCTTCCCACGTAGCGATCGGCCACTCATCAACATGATTCATGTTTCCTGTGGCATCTCAATTCTCGTGCTGATGGTCGTTCGTCTTTTGTTAAGGCTGAAATACCCAACTCCGCCGATTATACCTAAGCCAAAACCGATGATGACGGGACTGGCGCATTTGGGACATTTGGTGATTTATCTCCTGTTTATTGCGCTGCCAGTGa < 1:199273/249‑1 (MQ=255) aTCGCAGCGTCTTGAGCCATGGAGTTTCGTGGTTTCTTCCCACGTAGCGATCGGCCACTCATCAACATGATTCATGTTTCCTGTGGCATCTCAATTCTCGTGCTGATGGTCGTTCGTCTTTTGTTAAGGCTGAAATACCCAACTCCGCCGCTTATACCTACGCCAAAACCGATGATGACGGGACTGGCGCATTTGGGACATTTGGt > 2:257787/1‑206 (MQ=255) aTCGCAGCGTATTGCGCAATGGAGTTTCGTGGTTTCTTCCCACGTAGCGATCGGCCACTCATCAACATGATTCATGTTTCCTGTGGCATCTCAATTCTCGTGCTGATGGTCGTTCGTCTTTTGTTAAGGCTTAAATACCCAACTCCGCCGATTATACCTAAGCCAAAACCGATGATGACGGGACTGGCGCATTTGGGACATTTGGt < 1:257787/206‑1 (MQ=255) aTTGCGCAATTGAGGTTCGGGGTGTCTGCCCCCGTAGAGATCGGTCACTCATCAACATGATTCATGTTTCCTGTGGCGTCTCAATGCTCGTGCTGATGGTCGTTATAATATTGTTAAGGCTGAAATTCCCAACTCCGCAGATTATACCTAAGCCAAAACCGATGATGTGGGGACTGGCGCATGTGGGACATTTGGGGATTTATTTCCTGTTTATTGCGCTGCCAGTGATTGGTTTGGTGAGGATGTATAAc < 2:177235/251‑1 (MQ=255) tGCGCAATGGAGTTTCGTGGTTTCTTCCCACGTAGCGATCGGCCACTCATCAACATGATTCATGTTTCCTGTGGCATCTCAATTCTCGTGCTGATGGTCGTTCGTCTTTTGTTAAGGCTGAAATACCCAACTCCGCCGATTATACCTAAGCCAAAACCGATGATGACGGGACTGGCGCATTTGGGACATTTGGTGATTTATCTCCTGTTTATTGCGCTGCCAGTGATTGGTTTGGTGATGATGTATAAcc < 1:71158/250‑1 (MQ=255) gAGTTTCGTGGTTTCTTCCCACGTAGCGATCGGCAAATCATCAACATGATTCATGTTTCCTGAGGCATATCAATTCTCGTGCTGATGGTCGTTCGTATTTTGATAAGGCTGAAATACCAAACTCCGCCGATTATACCTAAGCCAAAACCGATGATGACGGGAAAGGCGCATTTGGGACATTTGGAGATTTATCTCCTGTTTATTGCGCTGCCAGTGATTGGTTTGGAGATGATGAATAACCGGGTCAAccc > 2:514147/1‑251 (MQ=255) gtgTTTCTTCCCGCGTAGCGAGCGGCCACTCATCAACATGATTCATGGTTCCTGTGGCAGCTCAAGTCTCGTGCTGATGGGCGTGCGGCGTTGGTTAAGGCGTAAATACCCAACTCCGCCGATTATACCTAAGCCAAAACCGATGATGACGGGACTGGCGCATTTTGGGCATTTGGTGATTTATCTCCTGTTTATTGCGCTGCCAGTGATTTGCTTGGTGAGGATGTATAACCGGGGCAACCCGTGGTTTg < 2:87911/249‑1 (MQ=255) ttcCCACGTAGCGATCGGCCACTCATCAACATGATTCATGTTTCCTGTGGCATCTCAATTCTCGTGCTGATGGTCGTTCGTCTTTTGTTAAGGCTGAAATACCCAACTCCGCCGATTATACCTAAGCCAAAACCGAtgatga < 2:173313/142‑1 (MQ=255) ttcCCACGTAGCGATCGGACACTCATCAACATGATTCATGTTTCCTGTGGCATCTCAATTCTCGTGCTGATGGTCGTTCGTCTTTTGTTAAGGCTGAAATACCCAACTCCGCCGATTATACCTAAGCCAAAACCGAtgatga > 1:173313/1‑142 (MQ=255) aCGTAGCGATCGGACACTCATCAAGAAGATTCATGTTTCCTGTGGCATCTCAATTCCCGTGATGATGGACGATCGCCTTTGGTTACGGCCGAACTACCCAACTCACCCGATTCTAACTAAGCCAAAACCGATGATGACGGGACTGGCGCGTTTGGGCCATTTGGTGATTTATCTCCTGTTTATTGCGCTGCCAGCGATTGGTTTGGTGATGATGCATAACCAGGTCAACCCGTGGTTTCCgcttggtttg > 2:577409/1‑250 (MQ=255) cGGCCACTCATCAACATGATTCAGGTTGCCTGTGGCATCTCAATTCTCGTGCTGATGGTCGTTCGTCTTTTGTTAAGGCTGAAATACCCAACTCCGCCGATTATACCTAAGCCAAAACCGATGATGACGGGACTGGCGCATTTGGGACATTTGGTGATTTATCTCCTGTTTATTGCGCTGCCAGTGATTGGTTTGGTGATGATGTATAACCGGGGCTACCCGTGGTGTTCGTTTGGTTTGACGATGccgt < 2:418109/250‑3 (MQ=255) gCCACTCATCAACATGATGCATGTTTCCTGTGGCATCTCAATTCTCGTGCTGATGGTCGTTCGTCTTTTGTTAAGGCTGAAATACCCAACTCCGCCGATTATACCTAAGCCAAAACCGATGATGACGGGACTGGCGCATTTGGGACATTTGGTGATTTATCTCCTGTTTATTGCGCTGCCAGTGATTGGTTTGGTGATGATGTATAACCGGGGCAACCCGTGGTTTGCGTGTGGTTTGACGATGCCTTACg < 1:398143/251‑1 (MQ=255) tgtgGCATCTCAATTCTCGTGCTGATGGTCGTTCGTCTTTTGTTAAGGCTGAAATACCCAACTCCGCCGATTATACCTAAGCCAAAACCGATGATGACGGGACTGGCGCATTTGGGACATTTGGTGATTTATCTCCTGTTTATTGCGCTGCCAGTGATTGGTTTGGTGATGATGTATAACCGGGGCAAccc < 2:308905/191‑1 (MQ=255) tgtgGCATCTCAATTCTCGTGCTGATGGTCGTTCGTCTTTTGTTAAGGCTGAAATACCCAACTCCGCCGATTATACCTAAGCCAAAACCGATGATGACGGGACTGGCGCATTTGGGACATTTGGTGATTTATCTCCTGTTTATTGCGCTGCCAGTGATTGGTTTGGTGATGATGTATAACCGGGGCAAccc > 1:308905/1‑191 (MQ=255) tgGCATCTCAATTCTCGTGCTGATGGTCGTTCGTCTTTTGTTAAGGCTGAAATACCCAACTCCGCCGATTATACCTAAGCCAAAACCGATGATGACGGGACTGGCGCATTTGGGACATTTGGTGATTTATCTCCTGTTTATTGCGCTGCCAGTGATTGGTTTGGTGATGATGTATAACCGGGGCAACCCGTGGTTTGCGTTTGGTTTGACGATGCCTTACGCTTCAGAGGCCAATTTCGaa > 2:314510/1‑241 (MQ=255) tgGCATCTCAATTCTCGTGCTGATGGTCGTTCGTCTTTTGTTAAGGCTGAAATACCCAACTCCGCCGATTATACCTAAGCCAAAACCGATGATGACGGGACTGGCGCATTTGGGACATTTGGTGATTTATCTCCTGTTTATTGCGCTGCCAGTGATTGGTTTGGTGATGATGTATAACCGGGGCAACCCGTGGTTTGCGTTTGGTTTGACGATGCCTTACGCTTCAGAGGCCAATTTCGaa < 1:314510/241‑1 (MQ=255) cAATTCTCGTGCTGATGGTCGTTCGTCTTTTGTTAAGGCTGAAATACCCAACTCCGCCGATTATACCTAAGCCAAAACCGATGATGACGGGACTGGCGCATTTGGGACATTTGGTGATTTATCTCCTGTTTATTGCGCTGCCAGTGATTGGTTTGGTGATGATGTATAACCGGGGCAACCCGTGGTTTGCGTTTGGTTTGACGATGCCTTACGCTTCAGAGGCCAATTTCGAACGGGTAGATAGCTTa < 1:60399/248‑1 (MQ=255) aTTCTCGTGCTGATGGTCGTTCGTCTTTTGTTAAGGCTGAAATACCCAACTCCGCCGATTATACCTAAGCCAAAACCGATGATGACGGGACTGGCGCATTTGGGACATTTGGTGATTTATCTCCTGTTTATTGCGCTGCCAGTGATTGGGTTGGTGATGATGTATAACCGGGGCAACCCGTGGTTTGCGTTTGGTTTGACGATGCCTTACGCTTCAGAGGCCAATTTCGAACGGGTAGATAGCTTAAAGTc < 2:488859/251‑1 (MQ=255) aTGGTCGTTCGGCGTTTGTTAAGTCTGAAATACCCAACTCCGCCGATTATACCTAAGCCAAAACCGATGATGACGGGACTGGCGTATTTGGGACATTTGGTGATTTATCTCCTGTTTAGTGCGCTGCCAGTTATTGGTTTGGTGATGATTTATAACCGGGTCAACCCGTGGTTTGCGGTTGGTTTGACGATGCCTTACGCTTCAGAGGCCAATTTCGAACGGGTAGATAGCTTAAAGTCGTGGCATGAAAc < 1:396490/251‑1 (MQ=255) ggCTGAAATACCCAACTCCGCCGATTATACCTAAGCCAAAACCGATGATGACGGGACTGGCGCATTTGGGACATTTGGTGATTTATCTCCTGTTTATTGCGCTGCCAGTGATTGGTTTGGTGATGATGTATAACCGGGGCAACCCGTGGTTTGCGTTTGGTTTGACGATGCCTTACGCTTCAGAGGCCAATTTCGAACGGGTAGATAGCTTAAAGTCGTGGCATGAAACGCTGGCGAATCTGGGATAtttt < 2:20142/251‑1 (MQ=255) | GCCCGTTATGTTAACGGAAAATTATGTTAAAGCAGGAAATGTTATGGAAAATAAATATTCAAGGTTACAAATCAGCATTCACTGGCTGGTCTTTTTACTGGTTATCGCAGCGTATTGCGCAATGGAGTTTCGTGGTTTCTTCCCACGTAGCGATCGGCCACTCATCAACATGATTCATGTTTCCTGTGGCATCTCAATTCTCGTGCTGATGGTCGTTCGTCTTTTGTTAAGGCTGAAATACCCAACCCCGCCGATTATACCTAAGCCAAAACCGATGATGACGGGACTGGCGCATTTGGGACATTTGGTGATTTATCTCCTGTTTATTGCGCTGCCAGTGATTGGTTTGGTGATGATGTATAACCGGGGCAACCCGTGGTTTGCGTTTGGTTTGACGATGCCTTACGCTTCAGAGGCCAATTTCGAACGGGTAGATAGCTTAAAGTCGTGGCATGAAACGCTGGCGAATCTGGGATATTTT > NC_000913/1490859‑1491339 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |