Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,419,414	C→T	G15S (GGT→AGT)	ydaG ←	uncharacterized protein YdaG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,419,414	0	C	T	100.0%	102.6 / NA	33	G15S (GGT→AGT)	ydaG	uncharacterized protein YdaG
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (18/15);  total (18/15)

TGCTTCTCCAACCATCGGCGCGCACCAGTTTCGGTTTTAAATGTTTTGCTTTTGGTATACGTCATGGCAGTGAACGTTCCATCCTGGTTGGGGAACACGCCGCACACCAGGGATTCGTTGTTGCCGAGGTCGATTTTTTGCATTTTGCGAATCTCACATCTTGTTGCTACGTATAGCGACTTCTGCCTGCCAGAGATCCCAGTCGTTGCTGCGTAAAGCCTGCACAGCCTGGTTGTAAGTGATACCGCAACAATCCATCAAATACTGAACTACTTCGTAATGCACCATCTTATCTCTCCCCTTAACGCCGGGTGGCGGAACTAACTGCTGCACTGCAAAATTTGAATCCCGCCGTCATGTTCATACGCCTCGGGCTGGCTACTTAACCCCTTACCACTGCCTGGTAACTCGAAGTATTGCCCGGCGTTCTGTGGGGCGGGGTGGGTGGTATGCTGGAACTATAGGTAATGCCTAATTGATTG  >  NC_000913/1419169‑1419650                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                             ccgatcgccAACCATCGGCGCGCACCAGTTTCGGTTTTAAATGTTTTGCTTTTGGTATACGTCATGGCAGTGAACGTTCCATCCTGGTTGGGGAACACGCCGCACACCAGGGATTCGTTGTTGCCGAGGTCGATTTTTTGCATTTTGCGAATCTCACATCTTGTTGCTACGTATAGCGACTTCTGCCTGCCAGAGATCCCAGTCGTTGCTGCGTAAAGCCTGCACAGCCTTGTTGTAAGTGATACTGcaa                                                                                                                                                                                                                                          <  2:530573/243‑1 (MQ=255)        ccAACCATCGGCGCGCACCAGTTTCGGTTTTAAATGTTTTGCTTTTGGTATACGTCATGGCAGTGAACGTTCCATCCTGGTTGGGGAACACGCCGCACACCAGGGATTCGTTGTTGCCGAGGTCGATTTTTTGCATTTTGCGAATCTCACATCTTGTTGCTACGTATAGCGACTTCTGCCTGCCAGAGATCCCAGTCGTTGCTGCGTAAAGCCTGCACAGCCTGGTTGTAAGTGATACTGcaa                                                                                                                                                                                                                                          >  1:530573/1‑243 (MQ=255)                       cACCAGTTTCGGTTTTAAATGTTTTGCTTTTGGTATACGTCATGGCAGTGAACGTTCCATCCTGGTTGGGGAACACGCCGCACACCAGGGATTCGTTGTTGCCGAGGTCGATTTTTTGCATTTTGCGAATCTCACATCTTGTTGCTACGTATAGCGACTTCTGCCTGCCAGAGATCCCAGTCGTTGCTGCGTAAAGCCTGCACAGCCTGGTTGTAAGTGATACTGCAACAATCCATCAAATACTGAactac                                                                                                                                                                                                                   >  2:419643/1‑251 (MQ=255)                                          tGTTTTGCTTTTGGTATACGTCATGGCAGTGAACGTTCCATCCTGGTTGGGGAACACGCCGCACACCAGGGATTCGTTGTTGCCGAGGTCGATTTTTTGCATTTTGCGAATCTCACATCTTGTTGCTACGTATAGCGACTTCTGCCTGCCAGAGATCCCAGTCGTTGCTGCGTAAAGCCTGCACAGCCTGGTTGTAAGTGATACTGCAACAATCCATCAACTACTGAACTACTTCGTAATGCACCATCtt                                                                                                                                                                                                 >  1:20194/1‑250 (MQ=255)                                                 cTTTTGGTATACGTCATGGCAGTGAACGTTCCATCCTGGTTGGGGAACACGCCGCACACCAGGGATTCGTTGTTGCCGAGGTCGATTTTTTGCATTTTGCGAATCTCACATCTTGTTGCTACGTATAGCGACTTCTGCCTGCCAGAGATCCCAGTCGTTGCTGCGTAAAGCCTGCACAGCCTGGTTGTAAGTGATACTGCAACAATCCATCAAATACTGAACTACTTCGTAATGCACCATCTTATCTCTcc                                                                                                                                                                                         >  1:111658/1‑251 (MQ=255)                                                   tttGGTATACGTCATGGCAGTGAACGTTCCATCCTGGTTGGGGAACACGCCGCACACCAGGGATTCGTTGTTGCCGAGGTCGATTTTTTGCATTTTGCGAATCTCACATCTTGTTGCTACGTATAGCGACTTCTGCCTGCCAGAGATCCCAGTCGTTGCTGCGTAAAGCCTGCACAGCCTGGTTGTAAGTGATACTGCAACAATCCATCAAATACTGAACTACTTCGTAATGCACCATCTTATCTCTcc                                                                                                                                                                                         <  1:430110/249‑1 (MQ=255)                                                    ttGGTATACGTCATGGCAGTGAACGTTCCATCCTGGTTGGGGAACACGCCGCACACCAGGGATTCGTTGTTGCCGAGGTCGATTTTTTGCATTTTGCGAATCTCACATCTTGTTGCTACGTATAGCGACTTCTGCCTGCCAGAGATCCCAGTCGTTGCTGCGTAAAGCCTGCACAGCCTGGTTGTAAGTGATACTGCAACAATCCATCAAATACTGAACTACTTCGTAATGCACCATCTTATCTCTcccc                                                                                                                                                                                       <  1:494438/250‑1 (MQ=255)                                                                           cGTTCCATCCTGGTTGGGGAACACGCCGCACACCAGGGATTCGTTGTTGACGAGGTCGATTTTTTGCATTTTGCGAATCTCACATCTTGTTGCTACGTATCGCGACTTCTGCCTGCCAGAGATCCCAGTCGTTGCTGCGTAAAGCCTGCACAGCCTGGTTGTAAGTGATACTGCAACAATCCATCAAATACTGAACTACTTCGTAATGCACCATCTTATCTCTCCCCTTACCGCCGGGTGGCGGaactaac                                                                                                                                                               >  2:564075/1‑251 (MQ=255)                                                                              tCCATCCTGGTTGGGGAACACGCCGCACACCAGGGATTCGTTGTTGCCGAGGTCGATTTTTTGCATTTTGCGAATCTCACATCTTGTTGCTACGTATAGCGACTTCTGCCTGCCAGAGATCCCAGTCGTTGCTGCGTAAAGCCTGCACAGCCTGGTTGTAAGTGATACTGCAACAATCCATCAAATACTGAACTACTTCGTAATGCACCATCTTATCTCTCCCCTTAACGCCGGGTGGCGGAACTAActgc                                                                                                                                                            >  1:588391/1‑251 (MQ=255)                                                                                              aaCACGCCGCACACCAGGGATTCGTTGTTGCCGAGGTCGATTTTTTGCATTTTGCGAATCTCACATCTTGTTGCTACGTATAGCGACTTCTGCCTGCCAGAGATCCCAGTCGTTGCTGCGTAAAGCCTGCACAGCCTGGTTGTAAGTGATACTGCAACAATCCATCAAATACTGAACTACTTCGTAATGAACCATCTTATCTCTCCCCTTAACGCCGGGTGGCGGAACTAACTGCTGCACTGCAAAATTTg                                                                                                                                            >  2:149122/1‑251 (MQ=255)                                                                                                      gcACACCAGGGATTCGTTGTTGCCGAGGTCGATTTTTTGCATTTTGCGAATCTCACATCTTGTTGCTACGTATAGCGACTTCTGCCTGCCAGAGATCCCAGTCGTTGCTGCGTAAAGCCTGCACAGCCTGGTTGTAAGTGATACTGCAACAATCCATCAAATACTGAACTACTTCGTAATGCACCATCTTATCTCTCCCCTTAACGCCGGGTGGCGGAACTAACTGCTGCACTGCAAAATTTGAATCccgc                                                                                                                                    <  2:95487/251‑1 (MQ=255)                                                                                                      gcACACCAGGGATTCGTTGTTGCCGAGGTCGATTTTTTGCATTTTGCGAATCTCACATCTTGTTGCTACGTATAGCGACTTCTGCCTGCCAGAGATCCCAGTCGTTGCTGCGTAAAGCCTGCACAGCCTGGTTGTAAGTGATACTGCAACAATCCATCAAATACTGAACTACTTCGTAATGCACCATCTTATCTCTCCCCTTAACGCCGGGTGGCGGAACTAACTGCTGCACTGCAAAATTTGAATCccgc                                                                                                                                    >  1:490430/1‑251 (MQ=255)                                                                                                         cacCAGGGATTCGTTGTTGCCGAGGTCGATTTTTTGCATTTTGCGAATCTCACATCTTGTTGCTACGTATAGCGACTTCTGCCTGCCAGAGATCCCAGTCGTTGCTGCGTAAAGCCTGCACAGCCTGGTTGTAAGTGATACTGCAACAATCCATCAAATACTGAACTACTTCGTAATGCACCATCTTATCTCTCCCCTTAACGCCGGGTGGCGGaa                                                                                                                                                                    >  2:104859/1‑216 (MQ=255)                                                                                                         cacCAGGGATTCGTTGTTGCCGAGGTCGATTTTTTGCATTTTGCGAATCTCACATCTTGTTGCTACGTATAGCGACTTCTGCCTGCCAGAGATCCCAGTCGTTGCTGCGTAAAGCCTGCACAGCCTGGTTGTAAGTGATACTGCAACAATCCATCAAATACTGAACTACTTCGTAATGCACCATCTTATCTCTCCCCTTAACGCCGGGTGGCGGaa                                                                                                                                                                    <  1:104859/216‑1 (MQ=255)                                                                                                             aGGGATTCGTTGTTGCCGAGGTCGATTTTTTGCATTTTGCGAATCTCACATCTTGTTGCTACGTATAGCGACTTCTGCCTGCCAGAGATCCCAGTCGTTGCTGCGTAAAGCCTGCACAGCCTGGTTGTAAGTGATACTGCAACAATCCATCAAATACTGAACTACTTCGTAATGCACCATCTTATCTCTCCCCTTAACGCCGGGTGGCGGAACTAACTGCTGCACTGCAAAATTTGAATCCCGCCGTCATg                                                                                                                             >  1:147651/1‑251 (MQ=255)                                                                                                                          tgCCGAGGTCGATTTTTTGCATTTTGCGAATCTCACATCTTGTTGCTACGTATAGCGACTTCTGCCTGCCAGAGATCCCAGTCGTTGCTGCGTAAAGCCTGCACAGCCTGGTTGTAAGTGATACTGCAACAATCCATCAAATACTGAACTACTTCGTAATGCACCATCTTATCTCTCCCCTTAACGCCGGGTGGCGGAACTAACTGCTGCACTGCAAAATTTGAATCCCGCCGTCATGTTCATACGCCTCg                                                                                                                >  1:138471/1‑251 (MQ=255)                                                                                                                              gAGGTCGATTTTTTGCATTTTGCGAATCTCACATCTTGTTGCTACGTATAGCGACTTCTGCCTGCCAGAGATCCCAGTCGTTGCTGCGTAAAGCCTGCACAGCCTGGTTGTAAGTGATACTGCAACAATCCATCAAATACTGAACTACTTCGTAATGCACCATCTTATCTCTCCCCTTAACGCCGGGTGGCGGAACTAACTGCTGCACTGCAAAATTTGAATCCCGCCGTCATGTTCATACGCCTCGggct                                                                                                            <  1:4817/251‑1 (MQ=255)                                                                                                                                  tCGATTTTTTGCATTTTGCAAATCTCACATCTTGTTGCTACGTCTAGCGACTTCTGACTGCCAGAGATCCCAGTCGTTGCTGCGTACAGCCTGCACAGCCTGGTTGTAAGTGATACTGCAACAATCCATCAAATACTGAACTACTTCGTAATGCACCATCTTATCTCTCCCCTTAACGCCGGGTGGCGGAACTAACTGCTGCACTGCAATATTTGACTCCCGCCGTCATGTTCATACGCCTCGggctggct                                                                                                        >  2:501292/1‑251 (MQ=255)                                                                                                                                                      aaTCTCACATCTTGTTGTTACGTATAGCGACTTCTGCCGGCCAGAGATCCCAGTCGTTGCTGCGTAAAGCCTGCACAGCCTGGTTGTAAGTGATACTGCAACAATCCATCAAATACTGAACTACTTCGTAATGCACCATCTTATCTCTCCCCTTAACGCCGGGTGGCGGAACTAACTGCGGCACTGCAAAATTTGAATCCCGCCGTCATGTTCATACGCCTCGGGCTGGCTACTTAACCCCTTACCACTGc                                                                                    <  2:588391/251‑1 (MQ=255)                                                                                                                                                         ctcACATCTTGTTGCTACGTATAGCGACTTCTGCCTGCCAGAGATCCCAGTCGTTGCTGCGTAAAGCCTGCACAGCCTGGTTGTAAGTGATACTGCAACAATCCATCAAATACTGAACTACTTCGTAATGCACCATCTTATCTCTCCCCTTAACGCCGGGTGGCGGAACTAACTGCTGCACTGCAAAATTTGAATCCCGCCGTCATGTTCATACGCCTCGGGCTGGCTACTTAACCCCTTACCACTGCCTg                                                                                 >  1:368088/1‑251 (MQ=255)                                                                                                                                                                   gttgCTACGTATAGCGACTTCTGCCTGCCAGAGATCCCAGTCGTTGCTGCGTAAAGCCTGCACAGCCTGGTTGTAAGTGATACTGCAACAATCCATCAAATACTGAACTACTTCGTAATGCACCATCTTATCTCTCCCCTTAACGCCGGGTGGCGGAACTAACTGCTGCACTGCAAAATTTGAATCCCGCCGTCATGTTCATACGCCTCGGGCTGGCTACTTAACCCCTTACCACTGCCTGGTAACTCGa                                                                        >  1:272843/1‑250 (MQ=255)                                                                                                                                                                             ataGCGACTTCTGCCTGCCAGAGATCCCAGTCGTTGCTGCGTAAAGCCTGCACAGCCTGGTTGTAAGTGATACTGCAACAATCCATCAAATACTGAACTACTTCGTAATGCACCATCTTATCTCTCCCCTTAACGCCGGGTGGCGGAACTAACTGCTGCACTGCAAAATTTGAATCCCGCCGTCATGTTCATACGCCTCGGGCTGGCTACTTAACCCCTTACCACTGCCTGGTAACTCGAAGTATTGCCCg                                                             <  2:163818/251‑1 (MQ=255)                                                                                                                                                                                                   gaTCCCAGTCGTTGCTGCGTAAAGCCTGCACAGCCTGGTTGTAAGTGATACTGCAACAATCCATCAAATACTGAACTACTTCGTAATGCACCATCTTATCTCTCCCCTTAACGCCGGGTGGCGGACCTAACTGCTGCACTGCAAAATTTGCATCCCGCCGTCATGTTCATACGCCTCGGGCTGGCTACTTAACCCCTTACCACTGCCTGGTAACTCGAAGTATTGcc                                                               >  1:390501/1‑227 (MQ=255)                                                                                                                                                                                                   gaTCCCAGCCGTTGCGGCGTAAAGCCTGCACAGCCTTGTTGTAAGTGATACTGCAACAATCCATCAAATACGGAACTACTTCGGAAGGCACCACCTTATCGCTCCCCTTAACGCCGGGTGGCGGAACTAACTGCTGCACTGCAAAATTTGAACCCCGCCGTCATGTTCATACGCCGCGGGCTGGCTACTTAACCCCTCACCACTGCCTGGTAACTCGAAGTATTGcc                                                               <  2:390501/227‑1 (MQ=255)                                                                                                                                                                                                        cAGTCGTGGCTGCGTAAAGCCTGTACAGCCTGGTTGTAAGTGATACTTCAACAATCCATCAAATGCGGAACTACTGCGTAATGCCCCATCTTATCTCTCCCCTTAACGCCGGGTGGCGGAACTAACTGCTGCACTGCAAAATTTGAAGCCCGCCGTCATGTTCATACGCCTCGGGCTGGCTACTTAACCCCTTACCACTGCCTGGTAACTCGAAGTATTGCCCGGCGTTCTGTGGGGCGGGGTGGGTGGTa                                  <  2:25804/251‑1 (MQ=255)                                                                                                                                                                                                         aGTCGTTGCTGCGTAAAGCCTGCACAGCCTGGTTGTAAGTGATACTGCAACAATCCATCAAATACTGAACTACTTCGTAATGCACCATCTTATCTCTCCCCTTAACGCCGGGTTGCGGAACTAACTGCTGCACTGCAAAATTTGAATCCCGCCGTCATGTTCATACGCCTCGGGCTGGCTACTTAACCCCTTACCACTGCCTGGTAACTCGAAGTATTGCCCGGCGTTCTGTGGGGCGGGTTGGGTGGTAt                                 <  1:434823/251‑1 (MQ=255)                                                                                                                                                                                                            cGTTGCTGCGTAAAGCCTGCACAGCCTGGTTGTAAGTGATACTGCAACAATCCATCAAATACTGAACTACTTCGTAATGCACCATCTTATCTCTCCCCTTAACGCCGGGTGGCGGAACTAACTGCTGCACTGCAAAATTTGAATCCCGCCGTCATGTTCATACGCCTCGGGCTGGCTACTTAACCCCTTACCACTGCCTGGTAACTCGAAGTATTGCCCGGCGTTCTGTGGGGCGGGGTGGGTGGTATGCt                              <  1:134193/251‑1 (MQ=255)                                                                                                                                                                                                                     gTAAAGCCTGCACAGCCTGGTTGTAAGTGATACTGCAACAATCCATCAAATACTGAACTACTTCGTAATGCACCATCTTATCTCTCCCCTTAACGCCGGGTGGCGGAACTAACTGCTGAACTGCAAAATTTGAATCCCGCCGTCATGTTCATACGCCTCGGGCTGGCTACTTAACCCCTTACCACTGCCTGGTAACTCGAAGTATTGCCCGGCGTTATGTGGGGCGGGGGGGGTGGTATGCTGGAACtct                      >  1:278063/1‑248 (MQ=255)                                                                                                                                                                                                                           ccTGCACAGCCTGGTTGTAAGTGATACTGCAACAATCCATCAAATACTGAACTACTTCGTAATGCACCATCTTATCTCTCCCCTTAACGCCGGGTGGCGGAACTAACTGCTGCACTGCAAAATTTGAATCCCGCCGTCATGTTCATACGCCTCGGGCTGGCTACTTAACCCCTTACCACTGCCTGGTAACTCGAAGTATTGCCCGGCGTTCTGTGGGGCGGGGTGGGTGGTATGCTGGAACTATAGGTAAt               <  2:445854/251‑1 (MQ=255)                                                                                                                                                                                                                             tGCACAGCCTGGTTGTAAGAGATACTGCAACAATCCATCAAATACTGAACTACTTCGTAATGCACCATCTTATCTCTCCCCTTAACGCCGGGTGGCGGAACTAACTGCTGCACTGCAAAATTTGAATCCCGCCGTCATGTTCATACGCCTCGGGCTGGCTACTTAACCCCTTACCACTGCCTGGTAACTCAACGTATTGCCCGGCGTTCTGTGGGGCGGGGTGGGGGGTATGCTGGAACTATAgggaaagc             >  2:515974/1‑245 (MQ=255)                                                                                                                                                                                                                             cgCACAGCCTGGTTGTAAGTGATACTGTAACAATCCATCAAATACTGAACTCCTTCTTAACGCTCCATCTTATCGCTCCCCTTATCGCCGGTTGGCGGAACTAACTGCTGCACTGCAAAATTTGAATCCCGCCGTTATTTTCATACGTCTCGGGCTGGCTACTTAACCCCTTACCACTGCCTGGTAACTCTTAGTATTGCCCGGCGTTCTGTGGGGCGGGGTGGGTGGTATGCTGGAACTATAGGTAATGc             <  1:51637/250‑1 (MQ=255)                                                                                                                                                                                                                              gCACAGCCTGGTTGTAAGTGATACTGCAACAATCCATCAAATACTGAACTCCTTCGTAATGCACCATCTTATCTCTCCCCTTAACGCCGGGTGGCGGAACTAACTGCTGCACTGCAAAATTTGAATCCCGCCGTCATGTTCATACGCCTCGGGCTGGCTACTTAACCCCTTACCACTGCCTGGTAACTCGAAGTATTGCCCGGCGTTCTGTGGGGCGGGGTGGGTGGTATGCTGGAACTATAGGTAATg              <  2:89588/249‑1 (MQ=255)                                                                                                                                                                                                                                        gTTGTAAGTGATACTGCAACAATCCATCAAATACTGAACTACTTCGTAATGCACCATCTTATCTCTCCCCTTAACGCCGGACGGCGGAACTAACTGCTGCACTGCAAAATTTGAATCCCGCCGTCATGTTCATACGCCTCGGGATGGCTACTTAACCCCTTACCACTGCCTGGTAACTCGAAGTATTGCCCGGCGTTCTGTGGGGCGGGGAGGGTGGTATGCTGGAACTATAGGTAATGCCGAattgattg  >  2:342547/1‑251 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                             TGCTTCTCCAACCATCGGCGCGCACCAGTTTCGGTTTTAAATGTTTTGCTTTTGGTATACGTCATGGCAGTGAACGTTCCATCCTGGTTGGGGAACACGCCGCACACCAGGGATTCGTTGTTGCCGAGGTCGATTTTTTGCATTTTGCGAATCTCACATCTTGTTGCTACGTATAGCGACTTCTGCCTGCCAGAGATCCCAGTCGTTGCTGCGTAAAGCCTGCACAGCCTGGTTGTAAGTGATACCGCAACAATCCATCAAATACTGAACTACTTCGTAATGCACCATCTTATCTCTCCCCTTAACGCCGGGTGGCGGAACTAACTGCTGCACTGCAAAATTTGAATCCCGCCGTCATGTTCATACGCCTCGGGCTGGCTACTTAACCCCTTACCACTGCCTGGTAACTCGAAGTATTGCCCGGCGTTCTGTGGGGCGGGGTGGGTGGTATGCTGGAACTATAGGTAATGCCTAATTGATTG  >  NC_000913/1419169‑1419650

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑