Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,418,807	C→T	T46I (ACT→ATT)	sieB →	phage superinfection exclusion protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,418,807	0	C	T	100.0%	79.9 / NA	25	T46I (ACT→ATT)	sieB	phage superinfection exclusion protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (13/12);  total (13/12)

ATTTCACCACCTCCGGGCTTCGGTGGTCTCGGCTATACCCCTACAGCGAGAGCTTGTGTTAACATTTCAATACCCTTACAGTTGAGAGTTATTGATATGTTGGATGTATTTACTCCATTGTTGAAACTTTTTGCTAACGAGCCACTCGAAAGACTTATGTATACGATTATCATTTTTGGTCTCACTCTCTGGCTGATACCGAAAGAGTTTACTGTCGCATTCAATGCTTATACTGAAATACCTTGGCTCTTTCAGATTATCGTTTTTGCCTTTTCTTTCGTGGTCGCCATTTCCTTCTCAAGATTGCGAGCACATATTCAAAAGCATTATTCATTACTACCAGAGCAACGAGTATTGCTTCGTTTATCTGAGAAAGAAATCGCTGT‑ATTTAAAGATTTCCTTAAAACAGGAAATCTTATTATCACTTCTCCTTGCCGTAACCCGGTTATGAAAAAATTAGAACGGAAGGGCATCATTC  >  NC_000913/1418575‑1419052                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                       aTTTCACCACCTCCGGGCTTCGGTGGTCTCGGCTATACCCCTACAGCGCGAGCTTGTGTTAACATTTCAATACCCTTACAGTTGAGAGTTATTGATATGTTGGATGTATTTACTCCATTGTTGAAACTTTTTGCTAACGAGCCACTCGCAAGACTTATGTATACGATTATCATTTTTGGTCTCACTCTCTGGCTGATACCGAAAGAGTTTACTGTCGCATTCAATGCTTATATTGAAATACCTTGGCTCtt                                                                                                                                                                                                                                      >  1:337245/1‑251 (MQ=255)          ccTCCGGGCTTCGGTGGTCGCGGCTATACCCCTACAGCGAGATCTTGTGTTAACATTTCAATACCCTTACAGTTGAGAGTTATTGATATGTTGGATGTATTTACTCCTTTGTTGAAACTTTTTGCTAACGAGCCACTCGAAAGACTTATGTATACGATTATCATTTTTGGTCTCACTCTCTGGCTGATACCGAAAGAGTTTACTGTCGCATTCAATGCTTATATTGAAATACCTTGGCTCTTTCAGATTAt                                                                                                                                                                                                                             <  1:348892/251‑1 (MQ=255)           cTCCGGGCTTCGGTGGTCTCGGCTATACCCCTACAGCGAGAGCTTGTGTTAACATTTCAATACCCTTACAGTTGAGAGTTATTGATATGTTGGATGTATTTACTCCATTGTTGAAACTTTTTGCTAACGAGCCACTCGAAAGACTTATGTATACGATTATCATTTTTGGTCTCACTCTCTGGCTGATACCGAAAGAGTTTACTGTCGCATTCAATGCTTATATTGAAATACCTTGGCTCTTTCAGATTATc                                                                                                                                                                                                                            >  1:221137/1‑251 (MQ=255)               gggCTTCGGTGGTCTCGGCTATACCCCTACAGCGAGAGCTTGTGTTACAATTTAAATACCCTTACAGTTGAGAGTTATTGCTATGTTGGATGTATTTACTCCACTGTTGAAACTTTTTGCTAACGAGCCACTCGAAAGACTTATGTATACGATTATCATTTTTGGTCTCACTCTCTGGCTGATACCGAAAGAGTTTACTGTCGCATTCAATGCTTATATTGAAATACCTTGGCTCTTTCAGATTATCGttt                                                                                                                                                                                                                        >  1:37751/1‑251 (MQ=255)                    tcgttggtCTTGGCTATACCCCTACAGCGAGAGCTTGTGTTAACAGTTCAATCCCCTTCCAGTTTAAAGTTATTTATTTTTTGGATGTATTTAGTCCATTGTTGAAACTTTTTGCTAACGGGCCACTCGAAAGACTTATGTATACGATTATCATTTTTGGGCTCACTCTCTGGCTGATACCGAAAGATTTTACTGTGGCATTCAATGCTTATATTGAAATACCTTGGCTCTTTCAGATTATCGTTTTTg                                                                                                                                                                                                                     <  1:47240/245‑1 (MQ=255)                                   ataCCCCTACAGCGAGAGCTTGTGTTAACATTTCAATACCCTTACAGTTGAGAGTTATTGATATGTTGGATGTATTTACTCCATTGTTGAAACTTTTTGCTAACGAGCCACTCGAAAGACTTATGTATACGATTATCATTTTTGGTCTCACTCTCTGGCTGATACCGAAAGAGTTTACTGTCGCATTCAATGCTTATATTGAAATACCTTGGCTCTTTCAGATTATCGTTTTTGCCTTTTCTTTCGTTGTc                                                                                                                                                                                                    >  2:415540/1‑251 (MQ=255)                                                                         cccTTACAGGTGAGAGTTATTGATATGTTGGATTTATTTTCTCCATTGTTGAAACTTTTTGTTAACGAGCCACTCGAAAGACTTATGTATACGATTATCATTTTTGGTCTCACTCTCTGGCTGATACCGAAAGAGTTTACTGTCGCATTCAATGCTTATATTGAAATACCTTGGCTCTTTCAGATTATCGTTTTTGCCTTTTCTTTCGTGGTCGCCATTTCCTTCTCAAGATTGCGAGCACATATTCaaaa                                                                                                                                                              <  2:483479/251‑1 (MQ=255)                                                                               cAGTTGAGAGTTATTGATATGTTGGATGTATTTACTCCATTGTTGAAACTTTTTGCTAACGAGCCACTCGAAAGACTTATGTATACGATTATCATTTTTGGTCTCACTCTCTGGCTGATACCGAAAGAGTTTACTGTCGCATTCAATGCTTATATTGAAATACCTTGGCTCTTTCAGATTATCGTTTTTGCCTTTTCTTTCGTGGTCGCCATTTCCTTCTCAAGATTGCGAGCACATATTCAAAAGCatta                                                                                                                                                        <  1:483851/251‑1 (MQ=255)                                                                                           aTTGATATGTTGGATGTATTTACTCCATTGTTGAAACTTTTTGCTAACGAGCCACTCGAAAGACTTATGTATACGATTATCATTTTTGGTCTCACTCTCTGGCTGATACCGAAAGAGTTTACTGTCGCATTCAATGCTTATATTGAAATACCTTGGCTCTTTCAGATTATCGTTTTTGCCTTTTCTTTCGTGGTCGCCATTTCCTTCTCAAGATTGCGAGCACATAtt                                                                                                                                                                   <  2:76107/228‑1 (MQ=255)                                                                                           aTTGATATGTTGGATGTATTTACTCCATTGTTGAAACTTTTTGCTAACGAGCCACTCGAAAGACTTATGTATACGATTATCATTTTTGGTCTCACTCTCTGGCTGATACCGAAAGAGTTTACTGTCGCATTCAATGCTTATATTGAAATACCTTGGCTCTTTCAGATTATCGTTTTTGCCTTTTCTTTCGTGGTCGCCATTTCCTTCTCAAGATTGCGAGCACATAtt                                                                                                                                                                   >  1:76107/1‑228 (MQ=255)                                                                                                                          tgAAACTTTTTGCTAACGAGCCACTCGAAAGACTTATGTATACGATTATCATTTTTGGTCTCACTCTCTGGCTGATACCGAAAGAGTTTACTGTCGCATTCAATGCTTATATTGAAATACCTTGGCTCTTTCAGATTATCGTTTTTGCCTTTTCTTTCGTGGTCGCCATTTCCTTCTCAAGATTGCGAGCACATATTCAAAAGCattatt                                                                                                                                                      >  1:572346/1‑210 (MQ=255)                                                                                                                           gAAACTTTTTGCTAACGAGCCACTCGAAAGACTTATGTATACGATTCTCATTTTTGGTCTCACTCTCTGGCTGATACCGAAAGAGTTTACTGTCGCATTCAATGCTTATATTGAAATACCTTGGCTCTTTCAGATTATCGTTTTTGCCTTTTCTTTCGTGGTCGCCATTTCCTTCTCAAGATTGCGAGCACATATTCAAAAGCATTATTCATTACTACCAGAGCAACGCGTATTGCTTCGTTTATCTg                                                                                                               >  2:57592/1‑248 (MQ=255)                                                                                                                                           gAGCCACTCGAAAGACTTATGTATACGATTATCATTTTTGGTCTCACTCTCTGGCTGATACCGAAAGAGTTTACTGTCGCATTCAATGCTTATATTGAAATACCTTGGCTCTTTCAGATTATCGTTTTTGCCTTTTCTTTCGTGGTCGCCATTTCATTCTCAAGATTGCGAGCACATATTCAAAAGCATTATTCATTACTACCAGCGCAACGAGTATTGCTTCGTTTATCTGAGAAAGAAATCGCTGA‑Att                                                                                           >  2:494438/1‑251 (MQ=255)                                                                                                                                             gCCACTCGAAAGACTTATGTATACGATTATCATTTTTGGTCTCACTCTCTGGCTGATACCGAAAGAGTTTACTGTCGCATTCAATGCTTATATTGAAATACCTTGGCTCTTTCAGATTATCGTTTTTGCCTTTTCTTTCGTGGTCGCCATTTCCTTCTCAAGATTGCGAGCACATATTCAAAAGCATTATTCATTACTACCAGAGCAACGAGTATTGCTTCGTTTATCTGAGAAAGAAATCGCTGT‑ATTTa                                                                                         <  1:524517/251‑1 (MQ=255)                                                                                                                                                aCTCGAAAGACTTATGTATACGATTATCATTTTTGGTCTCACTCTCTGGCTGATACCGAAAGAGTTTACTGTCGCATTCAATGCTTATATTGAAATACCTTGGCTCTTTCAGATTATCGTTTTTGCCTTTTCTTTCGTGGTCGCCATTTCCTTCTCAAGATTGCGAGCACATATTCAAAAGCATTATTCATTACTACCAGAGCAACGAGTATTGCTTCGTTTATCTGAGAAAGAAATCGCTGT‑ATTTaa                                                                                        >  1:67729/1‑249 (MQ=255)                                                                                                                                                  tCGAAAGACTTATGTATACGATTATCATTTTTGGTCTCACTCTCTGGCTGATACCGAAAGAGTTTACTGTCGCATTCAATGCTTATATTGAAATACCTTGGCTCTTTCAGATTATCGTTTTTGCCTTTTCTTTCGTGGTCGCCATTTCCTTCTCAAGATTGCGAGCACATATTCAAAAGCATTATTCATTACTACCAGAGCAACGAGTATTGCTTCGTTTATCTGAGAAAGAAATCGCTGT‑ATTTaa                                                                                        >  1:512744/1‑247 (MQ=255)                                                                                                                                                   cGAAAGACTTATGTATACGATTATCATTTTTGGTCTCACTCTCTGGCTGATACCGAAAGAGTTTACTGTCGCATTCAATGCTTATATTGAAATACCTTGGCTCTTTCAGATTATCGTTTTTGCCTTTTCTTTCGTGGTCGCCATTTCCTTCTCAAGATTGCGAGCACATATTCAAAAGCATTATTCATTACTACCAGAGCAACGAGTATTGCTTCGTTTATCTGAGAAAGAAATCGCTGT‑ATTTAAAGAtt                                                                                   <  2:221137/251‑1 (MQ=255)                                                                                                                                                         actaaTGTATACGATTATCCTTTTTGGTCTCACTCTCTGGCTGATAGCGAAAGAGGTTACTGTTGCAGTCAATGCGTATATTGAAATACCTTGGCTCGTTCAGATGATCGTGTTTGCCTTTTCTTTCGTGGTCGCGATTTCCTTCTCAAGATTGCGAGCACATATTCAAAAGCATTATACATTACTACCAGAGCTACGAGTATTGCGTCGGTTATCTGAGAAAGAAAGCGC‑GTCATTTAAAGAGTGCCTTa                                                                             <  2:34904/247‑1 (MQ=255)                                                                                                                                                            tATGTATACGATTATCATTTTTGGTCTCACTCTCTGGCTGATACCGAAAGAGTTTACTGTCGCATTCAATGCTTATATTGAAATACCTTGGCTCTTTCAGATTATCGTTTTTGCCTTTTCTTTCGTGGTCGCCATTTCCTTCTCAAGATTGCGAGCACATATTCAAAAGCATTATTCATTACTACCAGAGCAACGAGTATTGCTTCGTTTATCTGAGAAATAAATCGCTGT‑ATTTAAAGATTTCCTTaaa                                                                           <  1:156920/250‑1 (MQ=255)                                                                                                                                                                    cGATTATCATTTTTGGTCTCACTCTCTGGCTGATACCGAAAGAGTTTACTGTCGCATTCAATGCTTATATTGAAATGCCTTGGCTTTTTCAGATTATCGTTTTTGCCTTTTCTTTCGTGGTCGCCATTTCCTTCTCAAGATTGCGAGCACATATTCAAAAGCATTATTCATTACTACCAGATCAACGAGTATTGCTTCGTTTATCTGAGAAAGAAATCGCTGT‑ATTTAAAGAGTTCCTTAAAACAGGAAAt                                                                  <  1:378598/251‑1 (MQ=255)                                                                                                                                                                     gATTATCATTTTTGGTCTCACTCTCTGGCTGATACCGAAAGAGTTTACTGTCGCATTCAATGCTTATATTGAAATACCTTGGCTCTTTCAGATTATCGTTTTTGCCTTTTCTTTCGTGGTCGCCATTTCCTTCTCAAGATTGCGAGCACATATTCAAAAGCATTATTCATTACTACCAGAGCAACGAGTATTGCTTCGTTTATCTGAGAAAGAAATCGCTGT‑ATTTAAAGATTTCCTTAAAACAGGAAATc                                                                 >  1:250038/1‑251 (MQ=255)                                                                                                                                                                                              tGGCTGATACCGAAAGAGTTTACTGTCGCATTCAATGCTTATATTGAAATACCTTGGCTCTTTCAGATTATCGTTTTTGCCTTTTCTTTCGTGGTCGCCATTTCCTTCTCAAGATTGCGAGCACATATTCAAAAGCATTATTCATTACTACCAGAGCAACGAGTATTGCTTCGTTTATCTGAGAAAGAAATCGCTGT‑ATTTAAAGATTTCCTTAAAACAGGAAATCTTATTATCACTTCTCCTTGCCGTa                                         >  2:263536/1‑250 (MQ=255)                                                                                                                                                                                                gCTGATACCGAAAGAGTTTACTGTCGCATTCAATGCTTATATTGAAATACCTTGGCTCTTTCAGATTATCGCTTTTGCCTTTTCTTTCGTGGTCGCCATTTCCTTCTCAAGATTGCGAGCACATATTCAAAAGCATTATTCATTACTACCAGAGCAACGAGTATTGCTTCGTTTATCTGAGAAAGAAATCGCTGT‑ATTTAAAGATTTCCTTAAAACAGGAAATCTTATTATCACTTCTCCTTGCCGTAAcc                                      >  1:241440/1‑251 (MQ=255)                                                                                                                                                                                                              agTTTACTTTCGCATTCAATGCTTATATTGAAATTCCTTGGCTCTGTCAGATTATCGTTTTTGCCTTTTCTTTCGTGGTCGCCATTTCCTTCTCAAGATTGCGAGCACATATTCAAAATCATTAGTCATTACTACCAGAGCAACGAGTATTGCTTCGTTTATCTGAGAAAGAAATCGCTGT‑ATTTAAAGATGTCCTTAAAACAGGAAATCTTATTATCACGTCCCCTTTCCGTAACCCGGTTAGTaaaaaa                        <  2:75809/251‑1 (MQ=255)                                                                                                                                                                                                                                     tataTTGAAATACCTTGGCTCTTTCAGATTATCGTTTTTGCCTTTTCTTTCGTGGTCGCCATTTCCTTCTCAAGATTGCGAGCACATATTCAAAAGCATTATTCATTACTACCAGAGCAACGAGTATTGCTTCGTTTATCTGAGAAAGAAATCGCTGT‑ATTTAAAGATTTCCTTAAAACAGGAAATCTTATTATCACTTCTCCTTGCCGTAACCCGGTTATGAAAAAATTAGAACGGAAGGGCATCATTc  <  1:263536/250‑1 (MQ=255)                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                       ATTTCACCACCTCCGGGCTTCGGTGGTCTCGGCTATACCCCTACAGCGAGAGCTTGTGTTAACATTTCAATACCCTTACAGTTGAGAGTTATTGATATGTTGGATGTATTTACTCCATTGTTGAAACTTTTTGCTAACGAGCCACTCGAAAGACTTATGTATACGATTATCATTTTTGGTCTCACTCTCTGGCTGATACCGAAAGAGTTTACTGTCGCATTCAATGCTTATACTGAAATACCTTGGCTCTTTCAGATTATCGTTTTTGCCTTTTCTTTCGTGGTCGCCATTTCCTTCTCAAGATTGCGAGCACATATTCAAAAGCATTATTCATTACTACCAGAGCAACGAGTATTGCTTCGTTTATCTGAGAAAGAAATCGCTGT‑ATTTAAAGATTTCCTTAAAACAGGAAATCTTATTATCACTTCTCCTTGCCGTAACCCGGTTATGAAAAAATTAGAACGGAAGGGCATCATTC  >  NC_000913/1418575‑1419052

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑