Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,405,569	C→T	R277W (CGG→TGG)	abgR →	putative LysR‑type DNA‑binding transcriptional regulator AbgR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,405,569	0	C	T	100.0%	102.7 / NA	33	R277W (CGG→TGG)	abgR	putative LysR‑type DNA‑binding transcriptional regulator AbgR
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (15/18);  total (15/18)

GACAATGCCGACGCCACACGGCAGCTACTACAAACAGTTGAGTGAATTGCTTGACGATCAGGCGCAAACGCCACAGGTCGGTGTAGTCTGCGAGACGTTCTCAGCCTGTATCAGTCTGGTGGCAAAAAGCGATTTTCTCAGCAAACTGCCTGAAGAAATGGGCTGCGATCCCTTGCACGGACAGGGGCTGGTGATGTTGCCGGTTAGCGAAATTTTACCGAAAGCGGCCTATTATTTGATTCAGCGGCGTGATAGTCGCCAGACACCACTGACCGCGTCATTAATCACGCAATTCCGGCGAGAATGCGGCTATCTGCAAAGTTAAAACTGCATAAAAAAATAGAGTCTGTCGACATCCGCCAGACTCTACAGTACACACAGCAGTGCATCCGCGTCTTAAATCCGGCATTGTCTCCTCTGCGCCGGTGACTGTGTTATAACGGTTTTAACCTTTAGTTGCCAATTTTCTTCAGTGACA  >  NC_000913/1405325‑1405802                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                          gACAATGCCGACGCCACACGGCAGCTACTACAAACAGTTGAGTGAATTGCTTGACGATCAGGCGCAAACGCCACAGGTCGGTGTAGTCTGCGAGACGTTCTCAGCCTGTATCAGTCTGGTGGCAAAAAGCGATTTTCTCAGCAAACTGCCTGAAGAAATGGGCTGCGATCCCTTGCACGGACAGGGGCTGGTGATGTTGCCGGTTAGCGAAATTTTACCGAAAGCGGCCTATTATTTGATTCAGTGGCGTg                                                                                                                                                                                                                                     >  1:238269/1‑251 (MQ=255)                               cAAACAGTTGAGTGAATTGCTTGACGATCAGCCGCAAACGCCACAGGTCGGTGTAGTCTGCGAGACGTTCTCAGCCTGTATCAGTCTGGTGGCAAAAAGCGATTTTCTCAGCAAACTGCCTGAAGAAATGGGCTGCGATCCCTTGCACGGACAGGGGCTGGTGATGTTGACGGTTAGCGAAATTTTACCGAAAGCGGCCTATTATTTGATTCAGTGGCGTGATAGTCGACAGACACCACTGACCGCGCCAt                                                                                                                                                                                                       >  1:440518/1‑251 (MQ=255)                                    aGTTGAGTGAATTGCTTGACGATCAGGCGCAAACGCCACAGGTCGGTGTAGTCTGCGAGACGTTCTCAGCCTGTATCAGTCTGGTGGCAAAAAGCGATTTTCTCAGCAAACTGCCTGAAGAAATGGGCTGCGATCCCTTGCACGGACAGGGGCTGGTGATGTTGCCGGTTAGCGAAATTTTACCGAAAGCGGCCTATTATTTGATTCAGTGGCGTGATAGTCGCCa                                                                                                                                                                                                                           >  1:317741/1‑226 (MQ=255)                                    aGTTGAGTGAATTGCTTGACGATCAGGCGCAAACGCCACAGGTCGGTGTAGTCTGCGAGACGTTCTCAGCCTGTATCAGTCTGGTGGCAAAAAGCGATTTTCTCAGCAAACTGCCTGAAGAAATGGGCTGCGATCCCTTGCACGGACAGGGGCTGGTGATGTTGCCGGTTAGCGAAATTTTACCGAAAGCGGCCTATTATTTGATTCAGTGGCGTGATAGTCGCCa                                                                                                                                                                                                                           <  2:317741/226‑1 (MQ=255)                                            gAATTGCTTGACGATCAGGCGCAAACGCCTCAGGTCGGTGTAGTCTGCGAGACGTTCTCAGCCTGTATCAGTCTGGTGGCAAAAAGCGATTTTCTCAGCAAACTGCCTGAAGAAATGGGCTGCGATCCCTTGCACGGACAGGGGCTGGTGATGTTGCCGGTTAGCGAAATTTTACCGAAAGCGGCCTATTATTTGATTCAGTGGCGTGATAGTCGCCAGACACCACTGACCGCGTCATTAATCACGCAAt                                                                                                                                                                                           <  2:141189/250‑1 (MQ=255)                                              aTTGCTTGACGATCAGGCGCAAACGCCACAGGTCGGTGTAGTCTGCGAGACGTTCTCAGCCTGTATCAGTCTGGTGGCAAAAAGCGATTTTCTCAGCAAACTGCCTGAAGAAATGGGCTGCGATCCCTTGCACGGACAGGGGCTGGTGATGTTGCCGGTTAGCGAAATTTTACCGAAAGCGGCCTATTATTTGATTCAGTGGCGTGATAGTCGCCAGACACCACTGACCGCGTCATTAATCACGCAATTcc                                                                                                                                                                                        <  2:442833/251‑1 (MQ=255)                                                    tGACGATCAGGCGCAAACGCCACAGGTCGGTGTAGTCTGCGAGACGTTCTCAGCCTGTATCAGTCTGGTGGCAAAAAGCGATTTTCTCAGCAAACTGCCTGAAGAAATGGGCTGCGATCCCTTGCACGGACAGGGGCTGGTGATGTTGCCGGTTAGCGAAATTTTACCGAAAGCGGCCTATTATTTGATTCAGTGGCGTGATAGTCGCCAGACACCACTGACCGCGTCATTAATCACGCAATTCCGGCg                                                                                                                                                                                    <  1:108730/249‑1 (MQ=255)                                                     gACGATCAGGCGCAAACGCCACAGGTCGGTGTAGTCTGCGAGACGTTCTCAGCCTGTATCAGTCTGGTGGCAAAAAGCGATTTTCTCAGCAAACTGCCTGAAGAAATGGGCTGCGATCCCTTGCACGGACAGGGGCTGGTGATGTTGCCGGTTAGCGAAATTTTACCGAAAGCGGCCTATTATTTGATTCAGTGGCGTGATAGTCGCCAGACACCACTGACCGCGTCATTAATCACGCAATTCCGGCg                                                                                                                                                                                    >  2:101985/1‑248 (MQ=255)                                                      aCGATCAGGCGCAAACGCCACAGGTCGGTGTAGGCTGCGAGACGTTCTCAGCCTGTATCAGTCTGGTGGCAAAAAGCGATTTTCTCAGCAAACTGCCTGAAGAAATGGGCTGCGATCCCTTGCACGGACAGGGGCTGGTGATGTTGCCGGTTAGCGAAATTTTACCGAAAGCGGCCTATTATTTGATTCAGTGGCGTGAGAGTCGCCAGACACCACTGACCGCGTCATTAATCACGCAATTCCGGCGAGaa                                                                                                                                                                                <  1:287832/251‑1 (MQ=255)                                                            aGGCGCAAACGCCACAGGTCGGTGTAGTCTGCGAGACGTTCTCAGCCTGTATCAGTCTGGTGGCAAAAAGCGATTTTCTCAGCAAACTGCCTGAAGAAATGGGCTGCGATCCCTTGCACGGACAGGGGCTGGTGATGTTGCCGGTTAGCGAAATTTTACCGAAAGCGGCCTATGATTGGATTCAGTGGCGTGATAGTCGCCAGACACCACTGACCGCGTCATTAATCACGCAATTCCGGCGAGAATGCGGc                                                                                                                                                                          <  1:247741/251‑1 (MQ=255)                                                              gggcAAACGCCAAAGTTCGGTGGAGTCTGCGAGACGTTCTCAGCCTGTATCAGTCTGGTGGCAAAAAGCGATTTTCTCAGCAAACTGCCTGAAGAAATGGGCTGCGATCCCTTGCACGGACAGGGGCTGGTGATGTTGCCGGTTAGCGAAATTTTACCGTAAGCGGCCTATTATTTGATTCAGTGGCGTGATAGTCGCCAGACACCGCTGATCGCGTCATTAGTCGCGCAATTCCGGCCAGAATGCGGCTa                                                                                                                                                                        <  2:285644/249‑1 (MQ=255)                                                                gcagaCGCCACAGGTCGGTGTAGTCTGCGAGACGTTCTCAGCCTGTATCAGTCTGGTGGCAAAAAGCGATTTTCTCAGCAAACTGCCTGAAGAAATGGGCTGCGATCCCTTGCACGGACAGGGGCTGGTGATGTTGCCGGTTAGCGAAATTTTACCGAAAGCGGCCTATTATTTGATTCAGTGGCGTGATAGTCGCCAGACACCACTGACCGCGTCATTAATCACGCAAt                                                                                                                                                                                           >  2:324456/5‑230 (MQ=255)                                                                gcagaCGCCACAGGTCGGTGTAGTCTGCGAGACGTTCTCAGCCTGTATCAGTCTGGTGGCAAAAAGCGATTTTCTCAGCAAACTGCCTGAAGAAATGGGCTGCGATCCCTTGCACGGACAGGGGCTGGTGATGTTGCCGGTTAGCGAAATTTTACCGAAAGCGGCCTATTATTTGATTCAGTGGCGTGATAGTCGCCAGACACCACTGACCGCGTCATTAATCACGCAAt                                                                                                                                                                                           <  1:324456/226‑1 (MQ=255)                                                                  aaaCGCCACAGGTCGGTGTAGTCTGCGAGACGTTCTCAGCCTGTATCAGTCTGGTGGCAAAAAGCGATTTTCTCAGCAAACTGACTGAAGAAATGGGCTGCGATCCCTTGCACGGACAGGGGCTGGTGATGTTGCCGGTTAGCGAAATTTTACCGAAAGCGGCCTATTATTTGATTCAGTGGCGTGATAGTCGCCAGACACCACTGACCGCGTCATTAATCACGCAATTCCGGCGAGAATGCGGCTATCTg                                                                                                                                                                    >  1:53795/1‑251 (MQ=255)                                                                               cGGTGTAGTCTGCGAGACGTTCTCAGCCTGTATCAGTCTGGTGGCAAAAAGCGATTTTCTCAGCAAACTGCCTGAAGAAATGGGCTGCGATCCCTTGCACGGACAGGGGCTGGTGATGTTGCCGGTTAGCGAAATTTTACCGAAAGCGGCCTATTATTTGATTCAGTGGCGTGATAGTCGCCAGACACCACTGACCGCGTCATTAATCACGCAATTCCGGCGAGAATGCGGCTATCTGCAAAGTTAAAAc                                                                                                                                                        <  1:60671/250‑1 (MQ=255)                                                                                               aCGTTCTCAGCCTGTATCAGTCTGGTGGCAAAAAGCGATTTTCGCAGCAAACTGCCTGAAGAAATGGGCTGCGATCCCTTGCACGGACAGGGGCTGGTGATGTTGCCGGTTAGCGAAATTTTACCGAAAGCGGCCTATTATTTGATTCAGTGGCGTGATAGTCGCCAGACACCACTGACCGCGTCATTAATCACGCAATTCCGGCGAGAATGCGGCTATCTGCAAAGTTAAAACTGCATAAAAAAATagag                                                                                                                                       <  2:80420/251‑1 (MQ=255)                                                                                                 gTTCTCAGCCTGTATCAGTCTGGTGGCAAAAAGCGATTTTCTCAGAAAACTGCCTGAAGAAATGGGCTGCGATCCCTTGCACGGACAGGGGCTGGTGATGTTGCCGGTTAGCGAAATTTTACCGCAAGCGGCCTATTATTTGATTCAGTGGCGTGATAGTCGCCAGACACCACTGACCGCGTCATTAATCACGCAATTCCGGCGAGAATTCGGCTATCTGCAAAGTTAAAACTGCATAAAAAACTAGAGTc                                                                                                                                     >  1:345579/1‑251 (MQ=255)                                                                                                                 aGTCTGGTGGCAAAAAGCGATTTTATCAGCAAACTGCCTGAAGAAATGGGCTGCGATCCCTTGCACGGACAGGGGGTGGTGATGGTGCCGGTTAGCGAAATTTTACCGAAAGCTGCCTATTATTTGATGCAGTGGCGTGATAGGCGCCAGACACCACGGACCGCGTCATTAATCACGCAATGCCTGCGAGAATGCGGCTATATGCAAAGGTAAAACCGCATAAAAAAATAGAGGCTGGCGACATCGGCCAg                                                                                                                     <  2:535389/251‑1 (MQ=255)                                                                                                                   tCTGGGGGCAAAAAGCGATTTTCTCAGCAAACTGCCTGAAGAAATGGGCTGCGATCCCTTGCACGGACAGGGGCTGGTGATGTTGCCGGTTAGCGAAATTTTACCGAAAGCGGCCTATTATTTGATTCAGTGGCGTGATCGTCGCCAGACACCACTGCCCGCGTCATTAATCACGCAATTCCGGCGAGAATGCGGCTATCGGCAAAGTTAAAACTGCAAAAAAAAATAAAGTCTGTCGACATCCGCCAGAc                                                                                                                   >  2:358613/1‑251 (MQ=255)                                                                                                                           cAAAAAGCGATTTTCTCAGCAAACTGCCTGAAGAAATGGGCTGCGATCCCTTGCACGGACAGGGGCTGGTGATGTTGCCGGTTAGCGAAATTTTACCGAAAGCGGCCTATTATTTGATTCAGTGGCGTGATAGTCGCCAGACACCACTGACCGCGTCATTAATCACGCAATTCCGGCGAGAATGCGGCTATCTGCAAAGTTAAAACTGCATAAAAAAATAGAGTCTGTCGACATCCGCCAGACTCTACAGt                                                                                                           >  1:437884/1‑251 (MQ=255)                                                                                                                                 gCGATTGTCTCAGCAAATCGCCTGAAGAAATGGGCTGAGATCCCTTGCACGGACAGGGGCTGGTAATGTTGCCGGGTAGCGAAATTTTACCGAAAGCGGCCTATTAGTTGATTCAGTGGCGTGATAGTCGACAGACACCTCTGACCTCGTCATGAATCACGCAATTCCGGCGTGAATGCGGCTATCTGCAAAGTTAAAACTGCATAAAAAAAAAGAGTATGTCGACAACCCCCAGACTCTACatcactcac                                                                                                     <  2:370539/251‑9 (MQ=255)                                                                                                                                        tctcAGCAAACTGCCTGAAGAAATGGGCTGCGATCCCTTGCACGGACAGGGGCTGGTGATGTTGCCGGTTAGCGAAATTTTACCGAAAGCGGCCTATTATTTGATTCAGTGGCGTGATAGTCGCCAGACACCACTGACCGCGTCATTAATCACGCAATTCCGGCGAGAATGCGGCTATCTGCAAAGTTAAAACTGCATAAAAAAATAGAGTCTGTCGACATCCGCCAGACTCTACAGTACACACAGCAGt                                                                                               <  1:314737/250‑1 (MQ=255)                                                                                                                                            aGCAAACTGCCTGAAGAAATGGGCTGCGATCCCTTGCACGGACAGGGGCTGGTGATGTTGCCGGTTAGCGAAATTTTACCGAAAGCGGCCTATTATTTGATTCAGTGGCGTGATAGTCGCCAGACACCACTGACCGCGTCATTAATCACGCAATTCCGGCGAGAATGCGGCTATCTGCAAAGTTAAAACTGCATAAAAAAATAGAGTCTGTCGCCATCCGCCAGACTCTACAGTACACACAGCAGTGCATc                                                                                          >  1:26579/1‑251 (MQ=255)                                                                                                                                                aaCTGCCTGAAGAAATGGGCTGCGATCCCTTGCACGGACAGGGGCTGGTGATGTTGCCGGTTAGCGAAATTTTACCGAAAGCGGCCTATTATTTGATTCAGTGGCGTGATAGTCGCCAGACACCACTGACCGCGTCATTAATCACGCAATTCCGGCGAGAATGCGGCTATCTGCAAAGTTAAAACTGCATAAAAAAATAGAGTCTGTCGACATCCGCCAGACTCTACAGTACACACAGCAGTGCATCcgcg                                                                                      >  2:298536/1‑251 (MQ=255)                                                                                                                                                    gCCTGAAGAAATGGGCTGCGATCCCTTGCACGGACAGGGGCTGGTGATGTTGCCGGTTAGCGAAATTTTACCGAAAGCGGCCTATTATTTGATTCAGTGGCGTGATAGTCGCCAGACACCACTGACCGCGTCATTAATCACGCAATTCCGGCGAGAATGCGGCTATCTGCAAAGTTAAAACTGCATAAAAAAATAGAGTCTGTCGACATCCGCCAGACTCTACAGTACACACAGCAGTGCATc                                                                                          >  1:395663/1‑243 (MQ=255)                                                                                                                                                    gCCTGAAGAAATGGGCTGCGATCCCTTGCACGGACAGGGGCTGGTGATGTTGCCGGTTAGCGAAATTTTACCGAAAGCGGCCTATTAGTTGATTCAGTGGCGTGATAGTCGCCAGACACCACTGACCGCGTCATTAATCACGCAATTCCGGCGAGAATGCGGCTATCTGCAAAGTTAAAACTGCATAAAAAAATAGAGTCTGTCGACATCCGCCAGACTCTACAGTACACACAGCAGTGCATc                                                                                          <  2:395663/243‑1 (MQ=255)                                                                                                                                                                       gATCCCTTGCACGGACAGGGGCTGGTGATGTTGCCGGTTAGCGAAATTTTACCGAAAGCGGACTATTATTTGATTCAGTGGCGTGCTAGTCGCCAGACACCACTGACCGCGTCATTAATCACGCAATTCCGGCGAGAATGCGGCTATCTGCAAAGTTAAAACTGCATAAAAAAATAGAGTCTGTCGACATCCGCCAGACTCTACAGTACACACAGCAGTGCATCCGCGTCTTAAATCCGGCATTGTctcct                                                               >  2:84110/1‑251 (MQ=255)                                                                                                                                                                               gCACGGACAGGGGCTGGTGATGGTGCCGGTTAGCGAAATTTTACCGAAAGCGGCCTATTAGTTGATTCAGTGGCGTGATAGTCGCCAGACACCACTGACCGCGTCATTAATCACGCAATTCCGGCGAGAATGCGGCTATCTGCAAAGTTAAAACTGCATAAAAAAATAGAGTCTGTCGACATCCGCCAGACTCTACAGTACACACAGCAGTGCATCCGCGTCTTAAATCCGGCATTGTCTCCTCTGCGcc                                                        <  1:358613/250‑1 (MQ=255)                                                                                                                                                                                                  aTGTTGCCGGTTAGCGAAATTTTACCGAAAGCGGCCTATTATTTGATTCAGTGGCGTGATAGTCGCCAGACACCACTGACCGCGTCATTAATCACGCAATTCCGGCGAGAATGCGGCTATCTGCAAAGTTAAAACTGCATAAAAAAATAGAGTCTGTCGACATCCGCCAGACTCTACAGCACACACAGCAGTGCATCCGCGTCTTAAATCCGGCATTGTCTCCTCTGCGCCGGTGACTGTGTTATAACGGt                                    <  1:327071/251‑1 (MQ=255)                                                                                                                                                                                                                         aCCGAAAGCGGCCTATTATTTGATTCAGTGGCGTGATAGTCGCCAGAAACCACTGACCGCGTCATTAATCACGCAATTCCGGCGAGAATGCGGCTATCTGCAAAGTTAAAAATGCATAAAAAAATAGAGTCTGTCGACATCCGCCAGACTCTACAGTACACACAGCAGTGCATCCGCGTCTTAAATCCGGCATTGTCTCCTCTGCGCCGGTGACTGTGTTATAACGGTTTTAACCTTTAGTTGCCAAtttt             >  1:381666/1‑251 (MQ=255)                                                                                                                                                                                                                              aaGCGGCCTATTATTTGATTCAGTGGCGTGATAGTCGCCAGACACCACTGACCGCGTCATTAATCACGCAATTCCGGCGAGAATGCGGCTATCTGCAAAGTTAAAACTGCATAAAAAAATAGAGTCTGTCGACATCCGCCAGACTCTACAGTACACACAGCAGTGCATCCGCGTCTTAAATCCGGCATTGTCTCCTCTGCGCCGGTGACTGTGTTATAACGGTTTTAACCTTTAGtt                      <  1:9970/237‑1 (MQ=255)                                                                                                                                                                                                                              aaGCGGCCTATTATTTGATTCAGTGGCGTGATAGTCGCCAGACACCACTGACCGCGTCATTAATCACGCAATTCCGGCGAGAATGCGGCTATCTGCAAAGTTAAAACTGCATAAAAAAATAGAGTCTGTCGACATCCGCCAGACTCTACAGTACACACAGCAGTGCATCCGCGTCTTAAATCCGGCATTGTCTCCTCTGCGCCGGTGACTGTGTTATAACGGTTTTAACCTTTAGtt                      >  2:9970/1‑237 (MQ=255)                                                                                                                                                                                                                                    ccTATTATTTGATTCAGTGGCGTGATAGTCGCCAGACACCACTGACCGCGTCATTAATCACGCAATTCCGGCGAGAATGCGGCTATCTGCAAAGTTAAAACTGCATAAAAAAATAGAGTCTGTCGACATCCGCCAGACTCTACAGTACACACAGCAGTGCATCCGCGTCTTAAATCCGGCATTGTCTCCTCTGCGCCGGTGACTGTGTTATAACGGTTTTAACCTTTAGTTGCCAATTTTCTTCAGTGACa  <  2:211822/251‑1 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                          GACAATGCCGACGCCACACGGCAGCTACTACAAACAGTTGAGTGAATTGCTTGACGATCAGGCGCAAACGCCACAGGTCGGTGTAGTCTGCGAGACGTTCTCAGCCTGTATCAGTCTGGTGGCAAAAAGCGATTTTCTCAGCAAACTGCCTGAAGAAATGGGCTGCGATCCCTTGCACGGACAGGGGCTGGTGATGTTGCCGGTTAGCGAAATTTTACCGAAAGCGGCCTATTATTTGATTCAGCGGCGTGATAGTCGCCAGACACCACTGACCGCGTCATTAATCACGCAATTCCGGCGAGAATGCGGCTATCTGCAAAGTTAAAACTGCATAAAAAAATAGAGTCTGTCGACATCCGCCAGACTCTACAGTACACACAGCAGTGCATCCGCGTCTTAAATCCGGCATTGTCTCCTCTGCGCCGGTGACTGTGTTATAACGGTTTTAACCTTTAGTTGCCAATTTTCTTCAGTGACA  >  NC_000913/1405325‑1405802

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑