Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	59,968	C→T	V127I (GTC→ATC)	rluA ←	23S rRNA pseudouridine(746) and tRNA pseudouridine(32) synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	59,968	0	C	T	100.0%	225.2 / NA	70	V127I (GTC→ATC)	rluA	23S rRNA pseudouridine(746) and tRNA pseudouridine(32) synthase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (39/31);  total (39/31)

CGCCGGATGGGTAATCGTCAACATCTCTGCATGTAGCAACAAACGTGGTGCCATCGCTCTCGCTTCTGGTGATGCATAAAAACGATCGCCGAGAATCGGATGACCCAGCGCCAGCATATGCACACGCAATTGATGCGAACGCCCGGTAATCGGTTTTAACACCACTCTTGCCGTGTTATCCGCCGCATACTCCACCACTTCATATTCCGTCTGCGCAGGTTTACCCGTTTCGTAACAGACTTTCTGTTTCGGGCGGTTTGGCCAGTCGCAAATCAGCGGCAGATCCACCAGACCTTCTGCGGGGGATGGATGCCCCCAGACGCGGGCCACATACTGCTTTTTCGGCTCGCGCTCGCGGAACTGGCGTTTTAACTCCCGCTCCGCGGCTTTGGTCAGCGCCACTACAATCACGCCGCTGGTAGCCATATCCAGACGATGCACCGATTCTGCCTGCGGATAATCACGCTGAATGCGCGTCATCA  >  NC_000913/59729‑60210                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                   cGCCGGATGGGTAATCGTCAACATCTCTGCATGTAGCAACAAACGTGGTGCCATCGCTCTCGCTTCTGGTGATGCATAACAACGATCGCCGAGAATCGGATGACCCAGCGCCAGCATATGCACACGCAATTGATGCGAACGCCCGGTAATCGGTTTTAACACCACTCTTGCCGTGTTATCCGCCGCATACTCCACCACTTCATATTCCGTCTGCGCAGGTTTACCCGTTTCGTAACAGATTTTATGTTTCg                                                                                                                                                                                                                                         >  1:515848/1‑251 (MQ=255)      gATGGGTAATCGTCAACATCTCTGCATGTAGCAACAAACGTGGTGCCATCGCTCTCGCTTCTGGTCATGCATAAAAACGATCGCCGAGACTCGGATGACCCAGCGCCAGCATATGCACACGCAATTGATGCGAACGCCCGGTAATCGGTTTTAACACCACTCTTTCCGTGTTATCCGCCGCATACTCCACCACTTCATATTCAGTCTGCGCCGGTTTAACCGTTTCGTAACAGATTTTCTGTTTCGGGCgg                                                                                                                                                                                                                                    >  1:93622/1‑251 (MQ=255)         gggTAATCGTCAACATCTCTGCATGTAGCAACAAACGTGGTGCCATCGCTCTCGCTTCTGGTGATGCATAAAAACGATCGCCGAGAATCGGATGACCCAGCGCCAGCATATGCACACGCAATTGATGCTAACGCCCGGTAATCGGTTTTAACACCACTCTTGCCGTGTTATCCGCCGCATACTCCACCACTTCATATTCCGTCTGCGCAGGTTTACCCGTTTCGTAACAGATTTTCTGTTTCGTGCGGtt                                                                                                                                                                                                                                  <  2:478968/250‑1 (MQ=255)                     aCATCTCTGCATGTAGCAACAAACGTGGTGCCATCGCTCTCGCTTCTGGTGATGCATAAAAACGATCGCCGAGAATCGGATGACCCAGCGCCAGCATATGCACACGCAATTGATGCGAACGCCCGGTAATCGGTTTTAACACCACTCTTGCCGTGTTATCCGCCGCATACTCCACCACTTCATATTCCGTCTGCGCAGGTTTACCCGTTTCGTAACAGATTTTCTGTTTCGGGCGGTTTGGCCAGTCGCa                                                                                                                                                                                                                      >  1:107240/1‑250 (MQ=255)                        tctcTGCATGTAGCAACAAACGTGGTGCCATCGCTCTCGCTTCTGGTGATGCATAAAAACGATCGCCGAGAATCGGATGACCCAGCGCCAGCATATGCACACGCAATTGATGCGAACGCCCGGTAATCGGTTTTAACACCACTCTTGCCGTGTTATCCGCCGCATACTCCACCACTTCATATTCCGTCTGCGCAGGTTTACCCGTTTCGTAACAGATTTTCTGTTTCGGGCGGTTTGGCCAGTCGCAAATc                                                                                                                                                                                                                  >  1:508532/1‑251 (MQ=255)                           ctGCATGTAGCAACAAACGTGGTGCCATCGCTCTCGCTTCTGGCGATGCATAAAAACGATCGCCGAGAATCGGATGACCCAGCGCCAGCATATGCACACGCAATTGATGCGAACGCCCGGTAATCGGTTTTAACACCACTCTTGCCGTGTTATCCGCCGCATACTCCACCACTTCATATTCCGTCTGCGCAGGTTTACCCGTTTCGTAACAGATTTTCTGTTTCGGGCGGTTTGGCCAGTCGCAAATCAGc                                                                                                                                                                                                               >  1:247308/1‑251 (MQ=255)                              cATGTAGCAACACACGTGGTGCCATCGCTCTCGCTTCTGGTGATGAATAAAAACGATCGCCGAGAATCGGATGACCCAGCGCCAGCATATGCACACGCAATTGATGCGAACGCCCGGTAATCGGTTTTAACACCACTCTTGCCGTGTTATCCGCCGCATACTCCACCACTTCATATTCCGTCTGCGCAGGTTTACCCGTTTCGTAACAGATTTTCTGTTTCGGGCGGTTTGGCCAGTCGCAAATCAGCGGc                                                                                                                                                                                                            >  2:274466/1‑251 (MQ=255)                              cATGTAGCAACAAACGTGGTGCCATCGCTCTCGCTTCTGGTGATGCATAAAAACGATCGCCGAGAATCGGATGACCCAGCGCCAGCATATGCACACGCAATTGATGCGAACGCCCGGTAATCGGTTTTAACACCACTCTTGCCGTGTTATCCGCCGCATACTCCACCACTTCATATTCCGTCTGCGCAGGTTTACCCGTTTCGTAACAGATTTTCTGTTTCGGGCGGTTTGGCCAGTCGCAAATCAGCGGc                                                                                                                                                                                                            >  1:576342/1‑251 (MQ=255)                              cATGTAGCAACAAACGTGGTGCCATCGCTCTCGCTTCTGGTGATGCATAAAAACGATCGCCGAGAATCGGATGACCCAGCGCCAGCATATGCACACGCAATTGATGCGAACGCCCGGTAATCGGTTTTAACACCACTCTTGCCGTGTTATCCGCCGCATACTCCACCACTTCATATTCCGTCTGCGCAGGTTTACCCGTTTCGTAACAGATTTTCTGTTTCGGGCGGTTTGGCCAGTCGCAAATCAGCGGc                                                                                                                                                                                                            >  2:448028/1‑251 (MQ=255)                                      aacaaACGTGGTGCCATCGCTCTCGCTTCTGGTGATGCATAAAAACGATCGCCGAGAATCGGATGACCCAGCGCCAGCATATGCACACGCAATTGATGCGAACGCCCGGTAATCGGTTTTAACACCACTCTTGCCGTGTTATCCGCCGCATACTCCACCACTTCATATTCCGTCTGCGCAGGTTTACCCGTTTCGTAACAGATTTTCTGTTTCGGGCGGTTTGGCCAGTCGCAAATCAGCGGCAGATccac                                                                                                                                                                                                    >  2:161068/1‑251 (MQ=255)                                                             cGCTTCTGGTGATGCATAAACACGATCGCCGAGAATCGGATGACCCAGCGCCAGCATATGCACACGCAATTGATGCGAACGCCCGGTAATCGGTTTTAACACCACTCTTTCCGTGTTATCCGCCGCATACTCCACCACTTCATATTCCGTCTGCGCAGGTTTACCCGTTTCGTAACAGATTTTCTGTTTCGGGCGGTTTGGCCAGTCGCAAAGCAGCGGCAGATCCACCAGTCCTGCTGCGGGggatggat                                                                                                                                                                             <  2:35298/251‑1 (MQ=255)                                                                    ggTGATGCATAAAAACGATCGCCGAGAATCGGATGACCCAGCGCCAGCATATGCACACGCAATTGATGCGAACGCCCGGTAATCGGTTTTAACACCACTCTTGCCGTGTTATCCGCCGCATACTCCACCACTTCATATTCCGTCTGCGCAGGTTTACCCGTTTCGTAACAGATTTTCTGTTTCGGGCGGTTTGGCCAGTCGCAAATCAGCGGCAGATCCACCAGACCTTCTGCGGGGGATGGATGcccc                                                                                                                                                                        <  2:107240/249‑1 (MQ=255)                                                                     gTGATGCATAAAAACGATCGCCGAGAATCGGATGACCCAGCGCCAGCATATGCACACGCAATTGATGCGAACGCCCGGTAATCGGTTTTAACACCACTCTTGCCGTGTTATCCGCCGCATACTCCACCACTTCATATTCCGTCTGCGCAGGTTTACCCGTTTCGTAACAGATTTTCTGTTTCGGGCGGTTTGGCCAGTCGCAAATCAGCGGCAGATCCACCAGACCTTCTGCGGGGGATGGATGCCCCCAg                                                                                                                                                                     <  1:338672/251‑1 (MQ=255)                                                                          gCATAAAAACGATCGCCGAGAATCGGATGACCCAGCGCCAGCATATGCACACGCAATTGATGCGAACGCCCGGTAATCGGTTTTAACACCACTCTTGCCGTGTTATCCGCCGCATACTCCACCACTTCATATTCCGTCTGCGCAGGTTTACCCGTTTCGTAACAGATTTTCTGTTTCGGGCGGTTTGGCCAGTCGCAAATCAGCGGCAGATCCACCAGACCTTCTGCGGGGGATGGATGCCCCCAGAcgc                                                                                                                                                                 <  1:161068/250‑1 (MQ=255)                                                                            aTAAAAACGATCGCCGAGAATCGGATGACCCAGCGCCAGCATATGCACACGCAATTGATGCGAACGCCCGGTAATCGGTTTTAACACCACTCTTGCCGTGTTATCCGCCGCATACTCAACCACTTCATATTCCGTCTGCGCAGGTTTACCCGTTTCGTAACAGATTTTCTGTTTCGGGCGGTTTGGCCAGTCGCAAATCAGCGGCAGATCCACCAGACCTTCTGCGGGGGATGGATGCCCCCAGACGCggg                                                                                                                                                              >  1:237423/1‑251 (MQ=255)                                                                                  aCGATCGCCGAGAATCGGATGACCCAGCGCCAGCATATGCACACGCAATTGATGCGAACGCCCGGTAATCGGTTTTAACACCACTCTTGCCGTGTTATCCGCCGCATACTCCACCACTTCATATTCCGTCTGCGCAGGTTTACCCGTTTCGTAACAGATTTTCTGTTTCGGGCGGTTTGGCCAGTCGCAAATCAGCGGCAGATCCACCAGACCTTCTGCGGGGGATGGATGCCCCCAGACGCGGGCCACAt                                                                                                                                                        <  1:48325/251‑1 (MQ=255)                                                                                     aTCGCCGAGACTCGGATGAACCAGCGCCAGCATATGCACACGCAATTGCTGCGAACGCCCGGTCATCGGTTTTAACACCACTCTTGCCGTGTTATCCACCGCGTACTCCACCACTTCATATTCCTTCTGCGCAGATTTACCCTTTTCGTAACAGATTTTCTGTATCGGGCGGGTTGTCCAGCCGCAAATCCGCGTCAGATCCACCAGGCCTTCTGCGGGGGATGTATGTCCCCAGACGCGGCCCCCATACt                                                                                                                                                     >  2:117039/1‑251 (MQ=255)                                                                                       cGCCGAGAATCGGATGACCAAGCGCCAGCATATGCACACGCAATTGATGCGAACGCCCGGTAATCGGTTTTAACACCACTCTTGCCGTGTTATCCGCCGCATACTCCACCACTTCATATGCCGTCTGCGCAGGTTTACCCGTTTCGTAACAGATTTTCTGTTTCGGGCGGTTTGGCCAGTCGCAAATCAGCGGCAGATCCACCAGACCTTCTGCGGGGGATGGATGCCCCCAGACGCGGGCCACATACTg                                                                                                                                                    <  2:172273/250‑1 (MQ=255)                                                                                           gagaATCGGATGACCCAGCGCCAGCATATGCACACGCAATTGATGCGAACGCCCGGTAATCGGTTTTAACACCACTCTTGCCGTGTTATCCGCCGCATACTCCACCACTTCATATTCCGTCTGCGCAGGTTTACCCGTTTCGTAACAGATTTTCTGTTTCGGGCGGTTTGGCCAGTCGCAAATCAGCGGCAGATCCACCAGACCTTCTGCGGGGGATGGATGCCCCCAGACGCGGGCCACATACTGCtttt                                                                                                                                               >  2:460698/1‑251 (MQ=255)                                                                                           gagaATCGGATGACCCAGCGCCAGCATATGCACACGCAATTGATGCGAACGCCCGGTAATCGGTTTTAACACCACTCTTGCCGTGTTATCCGCCGCATACTCCACCACTTCATATTCCGTCTGCGCAGGTTTACCCGTTTCGTAACAGATTTTCTGTTTCGGGCGGTTTGGCCAGTCGCAAATCAGCGGCAGATCCACCAGACCTTCTGCGGGGGATGGATGCCCCCAGACGCGGGCCACATACTGCtttt                                                                                                                                               >  2:470765/1‑251 (MQ=255)                                                                                                  ggATGACCCAGCGCCCGCATATGCACACGCAATTGATGCGAACGCCCGGTAATCGGTTTTAACACCACTCTTGCCGTGTTATCCGCCGCATACTCCACCACTTCATATTCCGTCTGCGCAGGTTTACCCGTTTCGTAACAGATTTTCTGTTTCGGGCGGTTTGGCCAGTCGCAAATCAGCGGCAGATCCACCAGACCTTCTGCGGGGGATGGATGCCCCCAGACGCGGGCCACATACTGCTTTTTCGgctc                                                                                                                                        >  1:29563/1‑251 (MQ=255)                                                                                                  ggATGACCCAGCGCCAGCATATGCACACGCAATTGATGCGAACCCCCGGTAATCGGTTTTAACACCACTCTTGCCGTGTTATCCGCCGCATACTCCACCACTTCATATTCCGTCTGCGCAGGTTTACCCGTTTCGTAACAGATTTTCTGTTTCGGGCGGTTTGGCCAGTCGCAAATCAGCGGCAGATCCACCAGACCTTCTGCGGGGGATTGATGCCCCCAGACGCGGGCCACATACTGCTTTTTCGgctc                                                                                                                                        <  2:348478/251‑1 (MQ=255)                                                                                                      gACCCAGCGCCAGCATATGCCCACGCAATTGATGCGAACGCCCGGTAATCGGTTTTAACACCACTCTTGCCGTGTTATCCGCCGCATACTCCACCACTTCATATTCCGTCTGCGCAGGTTTACCCGTTTCGTAACAGATTTTCTGTTTCGGGCGGTTTGGCCAGTCGCAAATCAGCGGCAGATCCACCAGACCTTCTGCGGGGGATGGATGCCCCCAGACGCGGGCCACATACTGCTTTTTCGgctcgcg                                                                                                                                     <  1:662/250‑1 (MQ=255)                                                                                                             cgcCAGCATATGCACACGCAATTGATGCGAACGCCCGGTAATCGGTTTTAACACCACTCTTGCCGTGTTATCCGCCGCATACTCCACCACTTCATATTCCGTCTGCGCAGGTTTACCCGTTTCGTAACAGATTTTCTGTTTCGGGCGGTTTGGCCAGTCGCAAATCAGCGGCAGATCCACCAGACCTTCTGCGGGGGATGGATGCCCCCAGACGCGGGCCCCATACTGCTTTTTCGGCTCGCGCTCGCgg                                                                                                                              >  1:58560/1‑250 (MQ=255)                                                                                                               ccAGCATATGCACCCGCAATTGATGCGAACGCCCGGTAATCGGTTTTAACACCACTCTTGCCGTGTTATCCGCCGCATACTCCACCACTTCCTATTCCGTCTGCGCAGGTTTACCCGTTTCGTAACAGATTTTCTGTTTCGGGCGGTTTGGCCAGTCGCAAATCAGCGGCAGATCCACCAGACCTTCTGCGGGGGATGGATGCCCCCAGACGCGGGCCACATACTGCTTTTTCGGCTCGCGCTCGCggca                                                                                                                            >  1:494854/1‑248 (MQ=255)                                                                                                                   cATATGCACACGCAATTGATGCGAACGCCCGGTAATCGGTTTTAACACCACTCTTGCCGTGTTATCCGCCGCATACTCCACCAATTCATATTCCGTCTGCGCAGGTTTACCCGTTTCGTAACAGATTTTCTGTTTCGGGCGGTTTGGCCAGTCGCAAATCAGCGGCAGATCCACCAGACCTTCTGCGGGGGATGGATGCCCCCAGACGCGGGCCACATACTGCTTTTTCAGCTCGAGCTCGCGGCACTggg                                                                                                                       >  2:454693/1‑250 (MQ=255)                                                                                                                    atatGCACACGCAATTGATGCGAACGCCCGGTAATCGGTTTTAACACCACTCTTGCCGTGTTATCCGCCGCATACTCCACCACTTCATATTCCGTCTGCGCAGGTTTACCCGTTTCGTAACAGATTTTCTGTTTCGGGCGGTTTGGCCAGTCGCAAATCAGCGGCAGATCCACCAGACCTTCTGCggg                                                                                                                                                                                     >  2:578366/1‑188 (MQ=255)                                                                                                                    atatGCACACGCAATTGATGCGAACGCCCGGTAATCGGTTTTAACACCACTCTTGCCGTGTTATCCGCCGCATACTCCACCACTTCATATTCCGTCTGCGCAGGTTTACCCGTTTCGTAACAGATTTTCTGTTTCGGGCGGTTTGGCCAGTCGCAAATCAGCGGCAGATCCACCAGACCTTCTGCggg                                                                                                                                                                                     <  1:578366/188‑1 (MQ=255)                                                                                                                    atatGCACACGCAATTGATGCGAACGCCCGGTAATCGGTTTTAACACCACTCTTGCCGTGTTATCCGCCGCATACTCCACCACTTCATATTCCGTCTGCGCAGGTTTACCCGTTTCGTAACAGATTTTCTGTTTCGGGCGGTTTGGCCAGTCGCAAATCAGCGGCAGATCCACCAGACCTTCTGCGGGGGATGGATGCCCCCAGACGCGGGCCACATACTGCTTTTTCGGCTCGCGCTCGCGGAACTGGCg                                                                                                                      >  1:208545/1‑251 (MQ=255)                                                                                                                     tatGCACACGCAATTGATGCGAACGCCCGGTAATCGGTTTTAACACCACTCTTGCCGTGTTATCCGCCGCATACTCCACCACTTCATATTCCGTCTGCGCAGGTTTACCCGTTTCGTAACAGATTTTCTGTTTCGGGCGGTTTGGCCAGTCGCAAATCAGCGGCAGATCCACCAGACCTTCTGCGGGGGATGGATGCCCCCAGACGCGGGCCACATACTGCTTTTTCgg                                                                                                                                           <  1:547259/229‑1 (MQ=255)                                                                                                                     tatGCACACGCAATTGATGCGAACGCCCGGTAATCGGTTTTAACACCACTCTTGCCGTGTTATCCGCCGCATACTCCACCACTTCATATTCCGTCTGCGCAGGTTTACCCGTTTCGTAACAGATTTTCTGTTTCGGGCGGTTTGGCCAGTCGCAAATCAGCGGCAGATCCACCAGACCTTCTGCGGGGGATGGATGCCCCCAGACGCGGGCCACATACTGCTTTTTCgg                                                                                                                                           >  2:547259/1‑229 (MQ=255)                                                                                                                                         gagcGCCCGATAATCGGTGTTAACACCACTCGTGCCGGGTTATCCGCCGCATGCTCCACCACTTCATATTCCGCCTGCGCAGGTTTACCCGGTTCGTTACAGATTTTCTGTTTCGGGCGGTTTGGCCAGTCGCAAATCAGCGGCAGATCCACCAGGCCTTCTGCGGGGGATGTATGCCCCCAGACGCGGGCCTCATACTGCTTTTTCGGCTCGCGCTCGCGGAACTGGCGTTTTAACTCCCGCTCCGCGGc                                                                                                 <  2:518121/248‑1 (MQ=255)                                                                                                                                              cccGGTAATCGGTTTTAACACCACTCTTGCCGTGTTATCCGCCGCATACTCCACCACTTCATATTCCGTCTGCGCCGGTTTACCCGTTTCGTAACAGATTTTCTGTTTCGGGCGGTTTGGCCAGTCGCAAATCAGCGGCAGATCCACCAGACCTTCTGCGGGGGATGGATGCCCCCAGACGCGGGCCACATACTGCTTTTTCGGCTCGCGCTCGCGGAACTGGCGTTTTAACTCCCGCTCCGCGGCTTTgg                                                                                            >  1:74797/1‑251 (MQ=255)                                                                                                                                               ccGGTAATCGGTTTTAACACCACTCTTGCCGTGATATCCGACGCATACTCCACCACTTCATATTCCGTCTGCGCAGGTTTACCCGTTTCGTCACCGATTTTCTGTTTCGCGCGGTATGGCCAGTCGCAAATCAGCGGCAGATCCACCAGACCTTCTGCGGGGGATGGATGCCCCCAGACGCGGGCCACATACTGCTTTTTCGGCTCGAGCTCGCGGAAATGGCGTTTTAATTCCCGCTCCGCGGCTTTggg                                                                                           >  1:247311/1‑250 (MQ=255)                                                                                                                                                cGGTAATCGGTTTTAACACCACTCTTGCCGTGTGATCCGCCGCATACTCCACCACTTCATATTCCGTCTGCGCAGGTTTACCCGTTTCGTAACAGATTTTCTGGTTCGGGCGGTTTGGCCAGTCGCAAATCAGCGGCAGATCCACCAGACCTTCTGCGGGGGATGGATGCCCCCAGACGCGGGCCACATACTGCTTTTTCGGCTCGCGCTCGCGGAACTGGCGTTTTAACTCCCGCTCCGCGGCTTTGGTc                                                                                          <  2:238946/251‑1 (MQ=255)                                                                                                                                                cGGTAATCGGTTTTAACACCACACTTGCCGTGTTATCCGCCGCATACTCCACCACTTCATATTCCGTCAGCGCAGGTTTACCCGTTTCGTAACAGATTTTCTGTTTCGGGCGGTTTGGCCAGTCGCAAATCAGCGGCAGATCCACCAGACCTTCTGCGGGGGATGGATGCCCCCAGACGCGGGCCACATACTGCTTTTTCGGCTAGAGCTCGCGGCACTGGCGTTTTAACTCCCGCTCCGCGGCTTTGGTc                                                                                          >  1:221797/1‑251 (MQ=255)                                                                                                                                                 ggTAATCGGTTTTAACACCACTCTTGCCGTGTTATCCGCCGCATACTCCACCACTTCATATTCCGTCTGCGCAGGTTTACCCGTTTCGTAACAGATTGTCTGTTTCGGGCGGGTTGGCCAGTCGCAAATCAGCGGCAGATCCACCAGACCTTCTGCGGGGGATGGATGCCCCCAGACGCGGGCCACATACTGCTTTTTCGGCTCGCGCTCGCGGAACTGGCGTTTTAACTCCCGCTCCGCGGCTTTGGTCa                                                                                         <  1:397384/251‑1 (MQ=255)                                                                                                                                                  ctAATCGGTTTTAGCACCACTCTTGCAGTGTTATCCGCCGCATACTCCACCTCTTCATATTCCGTCTGCGCAGGTTTACCCGTTTCGTAACAGCTTTTCTGTTTCGATCGGTTGGGCCAGTCGCAAATCAGCGGCAGATCCACCAGTCCTTCTGCGGGGGATGGATGCCCCCAGACGCGGGCCACCTACTGCTTTTTCGGCTCGCGCGCGCGGGACCGGCGATTTAATCCCCCCTCCACGGCTTTggcccg                                                                                        >  2:882/2‑247 (MQ=255)                                                                                                                                                    aaTCGGTTTTAACACCACTCTTGCCTTGTTATCCGCCGCATACTCCACCACTTCATATTCCGTCTGCGCAGGTTTACCCGTTTCGTAACAGATTTTCTGTTTCGGGCGGTTTGGCCAGTCGCAAATCAGCGGCAGATCCACCAGACCTTCTGCGGGGGATGGATGCCCCCAGACGCGGGCCACATACTGCTTTTTCGGCTCGCGCTCGCGGAACTGGCGTTTTAACTCCCGCTCCGCGGCTTTGGTCAgcg                                                                                      <  1:413930/251‑1 (MQ=255)                                                                                                                                                        ggTTTTAACACCACTCTTGCCGTGTTATCCGCCGCATACTCCACCACTTCATATTCCGTCTGCGCAGGTTTACCCGTTTCGTAACAGATTTTCTGTTTCGGGCGGTTTGGCCAGTCGCAAATCAGCGGCAGATCCACCAGACCTTCTGCGGGGGATGGATGCCCCCAGACGCGGGCCACATACTGCTTTTTCGGCTCGCGCTCGCGGAACTGGCGTTTTAACTCCCGCTCCGCGGCTTTGGTCAGCGCCAc                                                                                  >  1:9552/1‑251 (MQ=255)                                                                                                                                                            ttAACACCACTCTTGCCGTGTTATCCGCCGCAGACTCCACCACTTCATATTCCGTCTGCGCAGGTTTACCCGTTTCGTAACAGATTTTCTGTTTCGGGCGGTTTGGCCAGTCGCAAATCAGCGGCAGAGCCACCAGACCTTCTGCGGGGGATGGATGCCCCCAGACGCGGGCCACATACTGCTTTTTCGGCTCGCGCTCGCGGAACTGGCGTTTTAACTCCCGCTCCGCGGCTTTGGTCAGCGCCACTAc                                                                               <  2:576342/250‑1 (MQ=255)                                                                                                                                                              aaCACCACTCTTGCCGTGTTATCCGCCGCATACTCCACCACTTCATATTCCGTCTGCGCAGGTTTACCCGTTTCGTAACAGATTTTCTGTTTCGGGCGGTTTGGCCAGTCGCAAATCAGCGGCAGATCCACCAGACCTTCTGCGGGGGATGGATGCCCCCAGACGCGGGCCACATACTGCTTTTTCGGCTCGCGCTCGCGGAACTGGCGTTTTAACTCCCGCTCCGCGGCTTTGGTCAGCGCCACTACAAt                                                                            <  2:508532/251‑1 (MQ=255)                                                                                                                                                                caccacTCTTGCCGTGTTATCCGCCGCATACTCCACCACTTCATATTCCGTCTGCGCAGGTTTACCCGTTTCGTAACAGATTTTCTGTTTCGGGCGGTTTGGCCAGTCGCAAATCAGCGGCAGATCCACCAGACCTTCTGCGGGGGATGGATGCCCCCAGACGCGGGCCACATACTGCTTTTTCGGCTCGCGCTCGCGGAACTGGCGTTTTAACTCCCGCTCCGCGGCTTTGGTCAGCGCCACTACAATc                                                                           >  1:370158/1‑250 (MQ=255)                                                                                                                                                                  ccacTCTTGCCGTGTTATCCGCCGCATACTCCACCACTTCATATTCCGTCTGCGCAGGTTTACCCGTTTCGTAACAGATTTTCTGTTTCGGGCGGTTTGGCCAGTCGCAAATCAGAGGCAGATCCACCAGACCTTCTGCGGGGGATGGATGCCCCCAGACGCGGGCCACATACTGCTTTTTCGGCTCGCGCTCGCGGGACTGGCGTTTTAACTCCCGCTCCGCGGCTTTGGTCAGCGCCACTACAATCAcg                                                                        >  1:75939/1‑251 (MQ=255)                                                                                                                                                                   cacTCTTGCCGTGTTATCCGCCGCATACTCCACCACTTCATATTCCGTCTGCGCAGGTTTACCCGTTTCGTAACAGATTTTCTGTTTCGGGCGGTTTGGCCAGTCGCAAATCAGCGGCAGATCCACCAGACCTTCTGCGGGGGATGGATGCCCCCAGACGCGGGCCACATACTGCTTTTTCGGCTCGCGCTCGCGGAACTGGCGTTTTAACTCCCGCTCCGCGGCTTTGGTCAGCGCCCCTACAATCAcgc                                                                       >  1:95478/1‑251 (MQ=255)                                                                                                                                                                    acTCTTGCCGTGTTATCCGCCGCATACTCCACCCCTTCATATTCCGTCTGCGCAGGTTTACCCGTTTCGTAACAGATTTTCTGTTTCGGGCGGTTTGGCCAGTCGCAAATCAGCGGCAGATCCACCAGACCTTCTGCGGGGGATGGATGCCCCCAGACGCGGGCCACATACTGCTTTTTCGGCTCGCGCTCGCGGAACTGGCGTTTTAACTCCCGCTCCGCGGCTTTGGTCAGCGCCACTACAATCAcgcc                                                                      >  1:104279/1‑251 (MQ=255)                                                                                                                                                                    acTCTTGCCGTGTTATCCGCCGCATACTCCACCACTTCATATTCCGTCTGCGCAGGTTTACCCGTTTCGTAACAGATTTTCTGTGTCGGGCGGTTTGGCCAGTCGCAAATCAGCGGCAGATCCACCAGACCTTCTGCGGGGGATGGATGCCCCCAGACGCGGGCCACATACTGCTTTTTCGGCTCGCGCTCGCGGAACTGGCGTTTTAACTCCCGCTCCGCGGCTTTGGTCAGCGCCACTACAATCAcgcc                                                                      <  2:526777/251‑1 (MQ=255)                                                                                                                                                                           ccgcgtTATCCGCCGCATACTCCACCACTTCATATTCCGTCTGCGCAGGTTTACCCGTTTCGTAACAGATTTTCTGTTTCGGGCGGTTTGGCCAGTCGCAAATCAGCGGCAGATCAACCAGACCTTCTGCGGGGGATGGATGCCCCCAGACGCGGGCCACATACTGCTTTTTCGGCTCGCGCTCGCGGAACTGGCGTTTTAACTCCCGCTCCGCGGCTTTGGTCAGCGCCACTACAATCACGCCGCTGGt                                                                >  1:89648/5‑250 (MQ=255)                                                                                                                                                                               gtTATCCGCCGCATACTCCACCACTTCATATTCCGTCTGCGCAGGTTTACCCGTTTCGTAACAGATTGTCTGTTTCGGGCGGTTTGGCCAGTCGCAAATCAGCGGCAGATCCACCAGACCTTCTGCGTGGGATGGATGCCCCCAGACGCGGGCCACATCCTGCTGTTTCGGCTCGCGCTCGCGGAACTGGCGGTTTAACTCCCGCTCCGCGGCGTGGGTCAGCGCGACTACAATCACGCCGCTGTTAGCCa                                                           <  2:247308/251‑1 (MQ=255)                                                                                                                                                                               gtTATCCGCCGCATACTCCACCACTTCATATTCCGTCGGCGCAGGTTTACCCGTTTCGTAACAGATTTTCTGTTTCGGGCGGTTTGGCCAGTCGCAAATCAGCGGCAGATCCACCAGACCTTCTGCGGGGGATGGATGCCCCCAGACGCGGGCCACATACTGCTTTTTCGGCTCGCGCTCGCGGAACTGGCGTTTTAACTCCCGCTCCGCGGCTTTGGTCAGCGCCACTACAATCACGCCGCTGGTAGcc                                                            <  2:18996/250‑1 (MQ=255)                                                                                                                                                                                 tATCCGCCGCATACTCCACCACTTCATATTCCGTCTGCGCAGGTTTACCCGTTTCGTAACAGATTTTCTGTTTCGGGCGGTTTGGCCAGTCGCAAATCAGCGGCAGATCCACCAGACCTTCTGCGGGGGATGGATGCCCCCAGACGCGGGCCACATACTGCTTTTTCGGCTCGCGCTCGCGGAACTGGCGTTTTAACTCCCGCTCCGCGGCTTTGGTCAGCGCCACTACACTCACGCCGCTGGTAGccctt                                                         >  2:75499/1‑248 (MQ=255)                                                                                                                                                                                        cgcATACTCCTCCACTTCATATTCGGTCTGCGCAGGTTTACCCGTTTCGTAACAGATTTTGTGTTTCGGGCGGTTTGGCCAGTCGCAAATCAGCGGCAGATCCACCAGACCTTCTGCGGGGGATGGAGGCCCCCAGACGCGGGCCACATACTGCTTTTTCGGCTCGCGCTCGCGGAACTGGCGTTTTAACTCCCGCTCCGCGGTTTTGGTCAGCGCCACTACAAGCACGCCTCTGGTAGCCATATCCAGAc                                                  <  2:54296/251‑1 (MQ=255)                                                                                                                                                                                           aTACTCCACCACGTCATATTCCGTCTGCGCAGGTTTACCCGTTTCGTAACAGATTTTCTGTTTCGGGCGGTTTGGCCAGTCGCAAATCAGCGGCAGATCCACCAGACCTTCTGCGGGGGATGGATGCCCCCAGACGCGGGCCACATACTGCTTTTTCGGCTCGCGCTCGCGGAACTGGCGTTTTAACTCCCGCTCCGCGGCTTTGGTCAGCGCCACTACATTCACGCCGCTGGTAGCCATATCCAGACGAt                                               <  1:448028/251‑1 (MQ=255)                                                                                                                                                                                                ccaccaCTTCATATTCCGTCTGCGCAGGTTTACCCGTTTCGTAACAGATTTTCTGTTTCGGGCGGTTTGGCCAGTCGCAAATCAGCGGCAGCTCCACCAGACCTTCTGCGGGGGATGGATGCCCCCAGACGCGGGCCACATACTGCTTTTTCGGCTCGCGCTCGCGGAACTGGCGTTTTAACTCCCGCTCCGCGGCTTTGGTCAGCGCCAATACAATCACGCCGCTGGTAGCCATATCCAGACAACGCAcc                                          >  1:321453/1‑251 (MQ=255)                                                                                                                                                                                                 caccacTTCCTATTCCGTCTGCGCAGGTTTACCCGTTTCGATACAGATTTTCTGTTTCGGGCGGTTGTGCCAGTCGCAAATCAGCGGCAGATCCACCAGACCTTCTGCGGGGGATGGATGCCCCCAGACGCGGGCCACATACTGCTTTTTCGGCTCGCGCTCGCGGAACTGGCGTTTTAACTCCCGCTCCGCGGCTTTGGTCAGCGCCACTACAATCACGCCGCTGGTAGCCATATCCAGACGATGCACCg                                         <  2:343874/251‑1 (MQ=255)                                                                                                                                                                                                  accacTTCAGATTCCGGCTGCGAATGTTTACCCGGTTCGTAAGCGATTTACTGTTTCGGGAGGTTGGGCCAGTCGCAAACCAGCGGCAGATCCACCAGTCCTTCTGCGGGGGATGGATGCCCCCAGACGCGGTCCAAATACTGCTTTGTCGGCCCGCGCTCGCGGAACTGGCGTTGTCACTCCCGCTCCGCGGCGTTGGTCAGCGCCCCTCCAATCACGACGCTGGTAGTCATATCCAGACGaaaacacga                                        <  2:322193/251‑9 (MQ=255)                                                                                                                                                                                                   ccacTTCATATTCCGTCTGCGCAGGATTACCCGTTTCGTAACAGATTTTCTGTTTCGGGCGGTTTGGCCAGTCGCAAATCAGCGGCAGATCCACCAGACCTTCTGCGGGGGATGGATGCCCCCAGACGCGGGCCACATACTGCTTTTTCGGCTCGCGCTCGCGGAACTGGCGTTT‑AACTCCCGCTCCGCGGCTTTGGTCCGCGCCACTACAATCACGCCGCTGGTAGCCATATCCAGACGATGCACCGAtt                                      >  2:586843/1‑251 (MQ=255)                                                                                                                                                                                                   ccacTTAATACTCCGTCTGCGCGGGTGTTCCCGTGTCGCTACAGATTTTCTGTTTCGGGCGGTTTGGACAGTCGCAAAACAGCGGCAGATCCACCAGACCTTCTGCGGGGGATGGATGCCCCCAGACGCGGGCCACATACTGCTTTGGCGGCTCGCGCCCGCGGCACTTGCGTTTTAACGCCCGCTCCGCTGCTTTGGTCAACGCCACTATAGTGAGGACGCGGGTAGCTATATCCAGACGATGCACCGAt                                       <  2:397612/251‑1 (MQ=255)                                                                                                                                                                                                         cATATTCCGTCTGCGCAGGTTTACCCGTTTCGTAACAGATTTTCTGTTTCGGGCGGTTTGGCCAGTCGCAAATCAGCGGCAGATCCACCAGACCTTCTGCGGGGGATGGATGCCCCCAGACGCGGGCCACATACTGCTTTTTCGGCTCGCGCTCGCGGAACTGGCGATTTAACTCCTGCTCCGCGGCTTTGGTCAACTCCACTACAATCACGCCGCTGGTAGCCCTATCCAGACGATGCACCGATTctgcc                                 >  1:210267/1‑251 (MQ=255)                                                                                                                                                                                                         cATATGCCGTCTGCGCAGGTTTACCCGTTTCGTAACAGATTTTCTGTTTCGGGCGGTTTGGCCAGTCGCAAATCAGCGGCAGATCCACCAGACCTTCTGCGGGG‑ATGGATGCCCCCAGACGCGGGCCACATACTGCTTTTTCGGCTCGCGCTCGCGGAACTGGCGTTTTAACTCCCGCGCCGCGGCTTTGGTCAGCGCCACTACAATCACGCCGTTGGTAGCCATATCCAGACGATTCACCGATTctgcct                                <  2:403451/251‑1 (MQ=255)                                                                                                                                                                                                                 gTCTGCGTAGGTTTACCCGGTTCGTAACAGATTTCCTGTTTCGCGCGGTTTGGCCAGTCGCAAATCAGCGGCAGATCCACCAGACCTGCTGCGGGGGATGGATGCCCCCAGACGCGGGCCACATACTGCTTGTGCGGCTCGCGCTCGCGGAACTGGCGTTTTAACTCCCGCTCCGCGGCTTTGGTCAGCGCCACTACAATCCCGCCGCTGGTAGCCATAGCCAGACTATGCACCGATTCTGCCTGCGGATa                         <  2:29563/251‑1 (MQ=255)                                                                                                                                                                                                                     gcgcAGGTTTACCCGTTTCGTAACAGATTTTCTGTTTCGGGCGGTTTGGCCAGTCGCAAATCAGCGGCAGATCCACCAGACCTTCTGCGGGGGATGGATGCCCCCAGACGCGGGCCACATACTGCTTTTTCGGCTCGCGCTCGCGGAACTGGCGTTTTAACTCCCGCTCCGCGGCTTTGGTCAGCGCCACTACAATCACGCCGCTGGTAGCCATATCCAGACGATGCACCGATTCTGCCTGCGGATAAt                       <  1:45022/249‑1 (MQ=255)                                                                                                                                                                                                                      cgcAGGTTTACCCGTTTCGTAACAGATTTTCTGTTTCGGGCGGTTTGGCCAGTCGCAAATCAGCGGCAGATCCACCAGACCTTCTGCGGGGGATGGATGCCCCCAGACGCGGGCCACATACTGCTTTTGCGGCTCGCGCTCGCGGAACTGGCGTTTTAACTCCCTCTCCGCGGCTTTGGTCAGCGCCACTACAATCACGCCTCTTGTAGCCATATCCAGACGATGCACCGATTCTGCCTTCGGATAATCAc                    <  1:390068/251‑1 (MQ=255)                                                                                                                                                                                                                       gcAGGTTTACCCGTTCCGTAACAGATTTTCGGTGTCGGGCGGTTTGGCCAGTCGCAAATCAGCGGCAGATCCACCAGACCTTCTGCGGGGGATGGATGCCCCCAGACGCGGGCCACATACTGCTTTTTCGGCTCGCGCTCGCGGAACTGGCGTTTTAACTCCCGCTCCGCGGCTTTGGTCAGCGCCACTACAACCACGCTGCTGGGAGCCATATCCAGACGATGCACCGATTCTGCCTGCGGATAATCACg                   <  1:208466/251‑1 (MQ=255)                                                                                                                                                                                                                        cAGGTTTACCCGTTTCGTAACAGATTTTCTGTTTCGGGCGGTTTGGCCAGTCGCAAATCAGCGGCAGATCCACCAGACCTTCTGCGGGGGATGGATGCCCCCAGACGCGGGCCACATACTGCTTTTTCGGCTCGCGCTCGCGGAACTGGCGTTTTAACTCCCGCTCCGCGGCTTTGGTCAGCGCCACTACAATCACGCCGCTGGTAGCCATATCCAGACGATGCACCGATTCTGCCTGCGGATAATCACGc                  <  1:249132/251‑1 (MQ=255)                                                                                                                                                                                                                           gattACCCGTTTCGAAACAGATTTTCTGTTTCGGGCGGTTTGGCCAGTCGCAAATCAGCGGCAGATCCACCAGACCTTCTGCGGGGGATGGATGCCCCCAGACGCGGGCCACATACTGCTTTTTCGGCTCGCGCTCGCGGAACTGGCGTTTTAACTCCCGCTCCGCGGCTTTGGTCAGCGCCACTAAAATCACGCCGCTGGTAGCCATATCCAGACGATGCACCGATTCTGCCTGCGGATAATCACGCTg                >  2:378990/3‑250 (MQ=255)                                                                                                                                                                                                                            tttACCCGTTTCGTAACAGATTTTCTGTTTCGGGCGGTTTGGCCAGTCGCAAATCAGCGGCAGATCCACCAGACCTTCTGCGGGGGATGGATGCCCCCAGACGCGGGCCACATACTGCTTTTTCGGCTCGCGCTCGCGGAACTGGCGTTTTAACTCCCGCTCCGCGGCTTTGGTCAGCGCCACTACAATCACGCCGCTGGTAGCCATATCCAGACGATGCACCGATTCTGCCTGCGGATAATCACGCTGa               >  2:43900/1‑250 (MQ=255)                                                                                                                                                                                                                                   gTTTCGTAACAGATTTTCTGTTTCGGGCGGTTTGGCCAGTCGCAAATCAGCGGCAGATCCACCAGACCTTCTGCGGGGGATGGATGCCCCCAGACGCGGGCCACATACTGCTTTTTCGGCTCGCGCTCGCGGAACTGGCGTTTTAACTCCCGCTCCGCGGCTTTGGTCAGCGCCACTACAATCACGCCGCTGGTAGCCATATCCAGACGCTGCACCGATTCTGCCTGCGGATAATCACGCTGAATGCGCGt       >  2:294722/1‑251 (MQ=255)                                                                                                                                                                                                                                      tCGTAACAGATTTTCTGTTTCGGGCGGTTTGGCCAGTCGCAAATAAGCGGCAGATCCACCAGACCTTCTGCGAGGGCAGAATGCCCCCAGAAACGGGCCACCTACTGCTTTTTCTCCACGCGCTCGCGGAAATGGCGTTTTAACTCCCGCTACGCGGCTTTGGTCAGCGCCACTAAACTCACGCCGCTGGTCGCCATATCCAGACGATGAAACGATTATGCCTGAGGATAATCACGCTGAATGCGCGtact    >  1:583556/1‑248 (MQ=255)                                                                                                                                                                                                                                        gTAACAGATTTTCTGTTTCGGGCGGTTTGGCCAGTCGCAAATCAGCGGCAGATCCACCAGACCTTCTGCGGGGGATGGATGCCCCCAGACGCGGGCCACATACTGCTTTTTCGGCTCGCGCTCGCGGAACTGGCGTTTTAACTCCCGCTCCGCGGCTTTGGTCAGCGCCACTACAATCACGCCGCTGGTAGCCATATCCAGACGATGCACCGATTCTGCCTGCGGATAATCACGCTGAATGCGCGtcatcc  >  1:71198/1‑250 (MQ=255)                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                   CGCCGGATGGGTAATCGTCAACATCTCTGCATGTAGCAACAAACGTGGTGCCATCGCTCTCGCTTCTGGTGATGCATAAAAACGATCGCCGAGAATCGGATGACCCAGCGCCAGCATATGCACACGCAATTGATGCGAACGCCCGGTAATCGGTTTTAACACCACTCTTGCCGTGTTATCCGCCGCATACTCCACCACTTCATATTCCGTCTGCGCAGGTTTACCCGTTTCGTAACAGACTTTCTGTTTCGGGCGGTTTGGCCAGTCGCAAATCAGCGGCAGATCCACCAGACCTTCTGCGGGGGATGGATGCCCCCAGACGCGGGCCACATACTGCTTTTTCGGCTCGCGCTCGCGGAACTGGCGTTTTAACTCCCGCTCCGCGGCTTTGGTCAGCGCCACTACAATCACGCCGCTGGTAGCCATATCCAGACGATGCACCGATTCTGCCTGCGGATAATCACGCTGAATGCGCGTCATCA  >  NC_000913/59729‑60210

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑