Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,395,566	C→T	S119S (TCG→TCA)	ynaI ←	small conductance mechanosensitive channel YnaI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,395,566	0	C	T	95.1%	129.7 / NA	42	S119S (TCG→TCA)	ynaI	small conductance mechanosensitive channel YnaI
Reads supporting (aligned to +/- strand):  ref base C (1/0);  major base T (16/23);  minor base G (1/1);  total (18/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCCAGCCAATTTCCGCTACTGTACCTTCGATATTTCTGTCCGGTGAACGGATCCAGTCGCCAATACTGAAAGGACGGTCGAAATAGAGCATAATCCCGGAAAAGAAGTTACTCAGAATATCTTTACCGGCCATACCGACAGCCAGACCACCAATACCACCAAAGGTCAGCAAGCCAGAAAGGCTCATGCCGAAATGTTCGCCATAAAGCAGAACAAGCACCACAATAATGGTGATTTTGATGATACGCGACATAATCCGCGCACTGGTGATATCGCGACCTTTTTTAATCTGCTGTTTTTCAAACTGATTAATCAGCAGAAATAGCTTAATCGTCAGAATAACCGCAATCAGGGACGTACAGATAAAATCGATAACGCCTGGGGTGATAAATTTGAGTTTATAGTTTTCTATAACATAATTAATAATGCTACCAACAGCACTGATAATTATGGTGTAGATTAAAAATTGCACCGCATGGAATAAAAATC  >  NC_000913/1395319‑1395807                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                  gccagccaATTTCAGCTACTGTACCTTCGATATTTCTGTCCGGTGAACGGATCCAGTCGCCAATACTGAAAGGACGGTCGAAATAGAGCATAATCCCGGAAGAGAAGTTACTCAGAATATCTTTACCGGCCATACCGACAGCCAGACCACCAATACCACCAAAGGTCAGCAAGCCAGAAAGGCTCATGCCGAAATGTTCGCCATAAAGCAGAACAAGCACCACAATAATGGTGATTTTGATGATACGTGAc                                                                                                                                                                                                                                                >  1:109750/1‑251 (MQ=255)  ccagccaATTTCCGCTACTGTACCTTCGATATTTCTGTCCGGTGAACGGATCCAGTCGCCAATACTGAAAGGACGGTCGAAATAGAGCATAATCCCGGAAAAGAAGTTACTCAGAATATCTTTACCGGCCATACCGACAGCCAGACCACCAATACCACCAAAGGTCAGCAAGCCAGAAAGGCTCATGCCGAAATGTTCGCCATAAAGCAGAACAAGCACCACAATAATGGTGATTTTGATGATACGTGAc                                                                                                                                                                                                                                                >  2:398675/1‑250 (MQ=255)   cagccaATTTCCGCTACTGTACCTTCGATATTTCTGTCCGGTGAACGGATCCAGTCGCCAATACTGAAAGGACGGTCGAAATAGAGCATAATCCCGGAAAAGAAGTTACTCAGAATATCTTTACCGGCCATACCGACAGCCAGACCACCAATACCACCAAAGGTCAGCAAGCCAGAAAGGCTCATGCCGAAATGTTCGCCATAAAGCAGAACAAGCACCACAATAATGGTGATTTTGATGATACGTGACAt                                                                                                                                                                                                                                              >  1:379972/1‑251 (MQ=255)            tCCGCTACTGTACCTTCGATATTTCTGTCCGGTGAACGGATCCAGTCGCCAATACTGAAAGGACGGTCGAAATAGAGCATAATCCCGGAAAAGAAGTTACTCAGAATACCTTTACCGGCCATACCGACAGCCAGACCACCAATACCACCAAAGGTCAGCAAGCCAGAAAGGCTCATGCCGAAATGTTCGCCATAAAGCAGAACAAGCACCACAATAATGGTGATTTTGATGATACGTCACATAATCcgcg                                                                                                                                                                                                                                      <  2:296039/250‑1 (MQ=255)                  aCTGTACCTTCGATATTTCTGTCCGGTGAACGGATCCAGTCGCCAATACTGAAAGGACGGTCGAAATAGAGCATAATCCCGGAAAAGAAGTTACTCAGAATATCTTTACCGGCCATACCGACAGCCAGACCACCAATACCACCAAAGGTCAGCAAGCCAGAAAGGCTCATGCCGAAATGTTCGCCATAAAGCAGAACAAGCACCACAATAATGGTGATTTTGATGATACGTGACATAATCCGCGCACTGGt                                                                                                                                                                                                                               <  2:126301/251‑1 (MQ=255)                  aCTGTACCTTCGATATTTCTGTCCGGTGAACGGATCCAGTCGCCAATACTGAAAGGACGGTCGAAATAGAGCATAATCCCGGAAAAGAAGTTACTCAGAATATCTTTACCGGCCATACCGACAGCCAGACCACCAATACCACCAAAGGTCAGCAAGCCAGAAAGGCTCATGCCGAAATGTTCGCCATAAAGCAGAACAAGCACCACAATAATGGTGATTTTGATGATACGTGACATAATCCGCGCACTGGt                                                                                                                                                                                                                               <  1:560550/251‑1 (MQ=255)                     gTACCTTCGATATTTCTGTCCGGTGAACGGATCCAGTCGCCAATACTGAAAGGACGGTCGAAATAGAGCATAATCCCGGAAAAGAAGTTACTCAGAATATCTTTACCGGCCATACCGACAGCCAGACCACCAATACCACCAAAGGTCAGCAAGCCAGAAAGGCTCATGCCGAAATGTTCGCCATAAAGCAGAACAAGCACCACAATAATGGTGATTTTGATGATACGTGACATAATCCGCGCACTGGTGat                                                                                                                                                                                                                            >  1:125642/1‑251 (MQ=255)                     gTACCTTCGATATTTCTGTCCGGTGAACGGATCCAGTCGCCAATACGGAAAGGACGGGCGAAATAGAGCATAATCCCGGAAAAGAAGTTACTCAGAATAGCTTTACCGGCCATACCGACAGCCAGACCACCAATACCACCAAAGGTCAGCAAGCCAGAAAGGCTCATGCCGAAAGGTTCGCCAGAAAGCAGAACAAGCACCACAATTATGGTGATTTTGATGATACGTGACATAATCCGCGCACTGGTGat                                                                                                                                                                                                                            <  1:576197/251‑1 (MQ=255)                             gATATTTCTGTCCGGTGAACGGAGCCAGTCGCCAATACTGAAAGGACGGTCGAAATAGAGCATAAACCCGGAAAAGAAGTTACTCAGAAAAGCTTTACCGGCCATACCGACAGCCAGACCACCAATACCACCAAAGGTCAGCAAGCCAGAAAGGCTCATGCCGAAATGTTCGCCATAAAGCAGAACAAGCACCACAATAATGGTGATTTTGAGGATACGTGACATAATCCGCGCACTGGTGATATCGCGAc                                                                                                                                                                                                                    <  2:109750/251‑1 (MQ=255)                                atTTCTGTCCGGTGAACGGATCCAGTCGCCAATACTGAAAGGACGGTCGAAATAGAGCATAATCCCGGAAAAGAAGTTACTCAGAATATCTTTACCGGCCATACCGACAGCCAGACCACCAATACCACCAAAGGTCAGCAAGCCAGAAAGGCTCATGCCGAAATGTTCGCCATAAAGCAGAACAAGCACCACAATAATGGTGATTTTGATGATACGTGACATAATCCGCGCACTGGTGATATCGCGACCtt                                                                                                                                                                                                                 <  1:276208/251‑1 (MQ=255)                                    cggTCCGGTGAACGGATCCAGTCGCCAATACTGAAAGGACGGTCGAAATAGAGCATAATCCCGGAAAAGAAGTTACTCAGAATATCTTTACCGGCCATACCGACAGCCAGACCACCAATACCACCAAAGGTCAGCAAGCCAGAAAGGCTCATGCCGAAATGTTCGCCATAAAGCAGAACAAGCACCACAATAATGGTGATTTTGATGATACGTGACATAATCCGCGCACTGGTGATATCGCGACCttttt                                                                                                                                                                                                              <  1:449559/248‑1 (MQ=255)                                          ggTGATCGGATCCAGTCGCCACTACTGAAAGGCCGGTCGAAATAGAGCATAATACCGGAAAAGAAGTTACTCAGAATATCTTTACCGGCCATACCGACAGCCAGACCACCAATACCACCAAAGGTCAGCAAGCCAGAAAGGCTCATGCAGAAATGTTCGCCATAAAGCAGAACAAGCACCACCATAATGGTGATTTTGATGATACGGAACATAATCCGCGAACTGGTGATATACCGACCTTTTTTAATctg                                                                                                                                                                                                       >  2:270960/1‑251 (MQ=255)                                              aaCGGATCCAGTCGCCAATACTGAAAGGACGGTCGAAATAGAGCATAATCCCGGAAAAGAAGTTACTCAGAATATCTTTACCGGCCATAAAGACAGCCAGACCACCAATACCCCCAAAGGTCAGCAAGCCAGAAAGGCTCATGCCGAAATGTTCGCCATAAAGCAGAACAAGCACCACAATAATGGTGATTTTGATGATACGTGACATAATCCGCGCACTGGTGATATCGCGACCTTTTTTAATCTGCTGt                                                                                                                                                                                                   >  1:543991/1‑251 (MQ=255)                                                cGGATCCAGTCGCCAATACTGAAAGGACGGTCGAAATAGAGCATAATCCCGGAAAAGAAGTTACTCAGAATATCTTTACCGGCCATACCGACAGCCAGACCACCAATACCACCAAAGGTCAGCAAGCCAGAAAGGCTCATGCCGAAATGTTCGCCATAAAGCAGAACAAGCACCACAATAATGGTGATTTTGATGATACGTGACATAAt                                                                                                                                                                                                                                           <  2:18520/209‑1 (MQ=255)                                                cGGATCCAGTCGCCAATACTGAAAGGACGGTCGAAATAGAGCATAATCCCGGAAAAGAAGTTACTCAGAATATCTTTACCGGCCATACCGACAGCCAGACCACCAATACCACCAAAGGTCAGCAAGCCAGAAAGGCTCATGCCGAAATGTTCGCCATAAAGCAGAACAAGCACCACAATAATGGTGATTTTGATGATACGTGACATAAt                                                                                                                                                                                                                                           >  1:18520/1‑209 (MQ=255)                                                             cAATACTGAAAGGACGGTCGAAATAGAGCATAATCCCGGAAAAGAAGTTACTCAGAATATCTTTACCGGCCATACCGACAGCCAGACCACCAATACCACCAAAGGTCAGCAAGCCAGAAAGGCTCATGCCGAAATGTTCGCCATAAAGCAGAACAAGCACCACAATAATGGTGATTTTGATGATACGTGACATAATCCGCGCACTGGTGATATCGCGACCTTTTTTAATCTGCTGTTTTTCAAACTGAtt                                                                                                                                                                                     >  1:184654/1‑250 (MQ=255)                                                                 aCTGAAAGGACGGTCGAAATAGAGAATAATCCCGGAAAAGAAGTTAATCAGAATATCTTTACCGGCCATACCGACAGCCAGACCACCAATACCACCAAAGGTCAGCAAGCCAGAAAGGCTCATGCCGAAATGTTCGCCATAAAGCAGAACAAGCACCACAATAATGGTGATTTTGATGATACGTGACATAATCCGCGCCCTGGTGATATCGATACCTTTTTTAATCTGCTGTTTTTCAAACTGATTAAta                                                                                                                                                                                 >  2:483714/1‑249 (MQ=255)                                                                                          aTAATCCCGGAAAAGAAGTTACTCAGAATATCTTTACCGGCCATACCGACAGCCAGACCACCAATACCACCAAAGGTCAGCAAGCCAGAAAGGCTCATGCCGAAATGTTCGCCATAAAGCAGAACAAGCACCACAATAATGGTGATTTTGATGATACGTGACATAATCAGCGCACTGGTGATATCGCGACCTTTTTTAATCTGCTGTTTTTCAAACTGATTAATCAGCAGAAATAGCTTAATCGTCAGAAt                                                                                                                                                       >  1:229370/1‑251 (MQ=255)                                                                                             aTCCCGGAAAAGAAGTTACTCAGAATATCTTTACCGGCCATACCGACAGCCAGACCACCAATACCACCAAAGGTCAGCAAGCCAGAAAGGCTCATGCCGAAATGTTCGCCATAAAGCAGAACAAGCACCACAATAATGGTGATTTTGATGATACGTGACATAATCCGCGCACGTGTGAGATCGCGACCTTTTTTAATCTGCTGTTTTTCAAACTGATTAATCAGCAGAAATAGCTTAATCGTCAGAATAAc                                                                                                                                                    <  1:276803/251‑1 (MQ=255)                                                                                               cccagAAAAGAATTTACTCATAATTACTTTCCCTGCCATACCGACAGCCAGACCACCAATACCACCAAATGACATCAAGCCAGACAGGCGCATGCCGAAATGTGCGCCATAAATCAGAACAAGCACCACAATTATGGTGATTGTGTTTATTCTTGACATAATTCGCGCACAGATGATATCGTGAAATTTTTTAATCTGCTGTGTTGCAAACTGATTAATCAGCAGATATAGCTTAATCATCAGAATAAcc                                                                                                                                                   <  2:289034/246‑1 (MQ=255)                                                                                                    aaaaGAAGTTACTCAGAATATCTGTACCGGCCATACCGACAGCCAGACCACCAATACCACCAAAGGTCAGCAAGCTAGAAAGGCTCATGCCGAAATGTTCGCCATAAAGCAGAACAAGCACCACAATAATGGTGATTTTGATGATACGTGACATAATCCGCGCACTGGTGATATCGCGACCTTTTTTAATCTGCTGTTTTTCAAACTGATTAATCAGCAGAAATAGCTTAATCGTCAGAATAACCGCAATc                                                                                                                                             <  1:587470/251‑1 (MQ=255)                                                                                                      aagaagTTACTCAGAATATCTTTACCGGCCATACCGACAGCCAGACCACCAATACCACCAAAGGTCAGCAAGCCAGAAAGGCTCATGCCGAAATGTTCGCCATAAAGCAGAACAAGCACCACAATAATGGTGATTTTGATGATACGTGACATAATCCGCGCACTGGTGATATCGCGACCTTTTTTAATCTGCTGTTTTTCAAACTGATTAATCAGCAGAAATAGCTTAATCGTCAGAATAACCGCAATCAg                                                                                                                                           <  2:256241/251‑1 (MQ=255)                                                                                                          agTTACTCAGAATATCTTTACCGGCCATACCGACAGCCAGACCACCAATACCACCAAAGGTCAGCAAGCCAGAAAGGCTCATGCCGAAATGTTCGCCATAAAGCAGAACAAGCACCACAATAATGGTGATTTTGATGATACGTGACATAATCCGCGCACTGGTGATATCGCGACCTTTTTTAATCTGCTGTTTTTCAAACTGATTAATCAGCAGAAATAGCTTAATCGTCAGAATAACCGCAATCAGGGAc                                                                                                                                       >  1:179999/1‑251 (MQ=255)                                                                                                              aCTCAGAATATCTTTACCGGCCATACCGACAGCCAGACCACCAATACCACCAAAGGTCAGCAAGCCAGAAAGGCTCATGCCGAAATGTTCGCCATAAAGCAGAACAAGCACCACAATAATGGTGATTTTGATGATACGTGACATAATCCGCGCACTGGTGATATCGCGACCTTTTTTAATCTGCTGTTTTTCAAACTGATTAATCAGCAGAAATAGCTTAATCGTCAGAATAACCGCAATCAGGGACGTa                                                                                                                                    <  2:439268/250‑1 (MQ=255)                                                                                                                      tatCTTTACCGGCCATACCGACAGCCAGACCACCAATACCACCAAAGGTCAGCAAGCCAGAAAGGCTCATGCCGAAATGTTCGCCATAAAGCAGAACAAGCACCACAATAATGGTGATTTTGATGATACGTGACATAATCCGCGCACTGGTGATATCGCGACCTTTTTTAATCTGCTGTTTTTCAAACTGATTAATCAGCAGAAATAGCTTAATCGTCAGAATAACCGCAATCAGGGACGTACAGATaaa                                                                                                                            >  2:365546/1‑250 (MQ=255)                                                                                                                                  ccATACCGACAGCCAGACCACCAATACCACCAAAGGTCAGCAAGCCAGAAAGGCTCATGCCGAAATGTTCGCCATAAAGCAGAACAAGCACCACAATAATGGTGATTTTGATGATACGTGACATAATCCGCGCACTGGTGATATCGCGACCTTTTTTAATCTGCTGTTTTTCAAACTGATTAATCAGCAGAAATAGCTTAATCGTCAGAATAACCGCAATCAGGGACGTACAGATAAAATCGATAACGCCt                                                                                                               <  1:450161/251‑1 (MQ=255)                                                                                                                                  ccATACCGACAGCCAGACCACCAATACCACCAAAGGTCAGCAAGCCAGAAAGGCTCATGCCGAAATGTTCGCCATAAAGCAGAACAAGCACCACAATAATGGTGATTTTGATGATACGTGACATAATCCGCGCACTGGTGATATCGCGACCTTTTTTAATCTGCTGTTTTTCAAACTGATTAATCAGCAGAAATAGCTTAATCGTCAGAATAACCGCAATCAGGGACGTACAGATAAAATCGATAACGCCt                                                                                                               <  1:34600/251‑1 (MQ=255)                                                                                                                                      aCCGACAGCCAGACCACCAATACCACCAAAGGTCAGCAAGCCAGAAAGGCTCATGCCGAAATGTTCGCCATAAAGCAGAACAAGCACCACAATAATGGTGATTTTGATGATACGTGACATAATCCGCGCACTGGTGATATCGCGACCTTTTTTAATCTGCTGTTTTTCAAACTGATTAATCAGCAGAAATAGCTTAATCGTCAGAATAACCGCAATCAGGGACGTACAGATAAAATCGATAACGCCTgggg                                                                                                           <  1:346045/251‑1 (MQ=255)                                                                                                                                                       caATACCACCAAAGGTCAGCAAGCCAGAAAGGCTCATGCCGAAATGTTCGCCATAAAGCAGAACAAGCGCCACAATAATGGTGATTTTGATGATACGTGACATAATCCGCGCACTGGTGATATCGCGACCTTTTTTAATCTGCTGTTTTTCAAACTGATTAATc                                                                                                                                                                                 <  2:139312/164‑1 (MQ=255)                                                                                                                                                         aTACCACCAAAGGTCAGCAAGCCAGAAAGGCTCATGCCGAAATGTTCGCCATAAAGCAGAACAAGCACCACAATAATGGTGATTTTGATGATACGTGACATAATCCGCGCACTGGTGATATCGCGACCTTTTTTAATCTGCTGTTTTTCAAACTGATTAATCAGCAGAAATAGCTTAATCGTCAGAATAACCGCAATCAGGGACGTACAGATAAAATCGATAACGCCTGGGGTGATAAATTTGAGTTtat                                                                                         >  2:119999/1‑250 (MQ=255)                                                                                                                                                          tACCACCAAAGGTCAGCAAGCCAGAAAGGCTCATGCCGAAATGTTCGCCATAAAGCAGAACAAGCACCACAATAATGGTGATTTTGATGATACGTGACATAATCCGCGCACTGGTGATATCGCGACCTTTTTTAATCTGCTGTTTTTCAAACTGATTAATCAGCAGAAATAGCTTAATCGTCAGAATAACCGCAATCAGGGACGTACAGATAAAATCGATAACGCCTGGGGTGATAAATTTGAGTTtat                                                                                         >  1:190615/1‑249 (MQ=255)                                                                                                                                                              accaAAGGTCAGCAAGCCAGAAATGCTCATGCCGAAATGGTCGCCATAAAGCAGAACAAGCACCACAATAATGGTGATTTTGATGATACGTGACATAATCCGCGCACTGGTGATATCGCGACCTTTGTTAAACTGCTGTGTTTCAAACTGATTAAGCAGCAGAAATAGCTTAATCGTCAGAAGAACCGCAATCAGGGACGTACAGATAAAATCTATAACGCCTTGGGTGATAAATTTGAGTTTATAGtttt                                                                                   <  2:298160/251‑1 (MQ=255)                                                                                                                                                                caAAGGTCAGCAATCCAGAAAGGCTCATGACGAAATGTTCGCCATAAAGCAGAACAAGCACCACAAGAATGGTGATTTTGATGATACGTGACATAATCCGCGCACTGTTGATATCGCGACCTTTTGTAAACTGCTGTTTTTCAAACTGATTAATCAGCAGAAATAGCTTAGTCGTCAGAATAACCTCTATCAGGGGCGTACAGATAAAAGCGATAACGCCTGGGGTGATAGTTTTGAGtgtttaggtttcg                                                                                 <  2:45171/251‑13 (MQ=255)                                                                                                                                                                          cAAGCCAGAAAGGCTCATGCCGAAATGTTCGCCATAAAGCAGAACAAGCACCACAATAATGGTGATTTTGATGATACGTGACATAATCCGCGCACTGGTGATATCGCGACCTTTTTTAATCTGCTGTTTTTCAAACTGATTAATCAGCAGAAATAGCTTAATCGTCAGAATAACCGCAATCAGGGACGTACAGATAAAATCGATAACGCCTGGGGTGATAAATTTGAGTTTATAGTTTTCTATAACAtaat                                                                       <  1:398675/251‑1 (MQ=255)                                                                                                                                                                          cAAGACAGAAAGGGTCATGCCGAAATGTTCGCCATAAAGCAGCACAAGCACCACAATAATGGTGATTTTGATGATACGGGACATAATCTGTGCATGGGTGATATCGCGACTTCTTTTAATATGCTGTTTTTCAAACTGATTAATCAGCAGAAATAGCTTAATCGTCAGAATAACCGCAATCAGGGACGTACAGATAAACTCGATAACGCCTGGGGTGATAAATTTGAGTTTATAGTGTTCTATAACAtaag                                                                       <  1:398686/251‑2 (MQ=255)                                                                                                                                                                                   aaGGCTCATGCCGAAATGTTCGCCATAAAGCAGAACAAGCACCACAATAATGGTGATTTTGATGATACGTGACATAATCCGCGCACTGGTGATATCGCGACCTTTTTTAATCTGCTGTTTTTCAAACTGATTAATCAGCAGAAATAGCTTAATCGTCAGAATAACCGCAATCAGGGACGTa                                                                                                                                    >  1:465111/1‑181 (MQ=255)                                                                                                                                                                                   aaGGCTCATGCCGAAATGTTCGCCATAAAGCAGAACAAGCACCACAATAATGGTGATTTTGATGATACGTGACATAATCCGCGCACTGGTGATATCGCGACCTTTTTTAATCTGCTGTTTTTCAAACTGATTAATCAGCAGAAATAGCTTAATCGTCAGAATAACCGCAATCAGGGACGTa                                                                                                                                    <  2:465111/181‑1 (MQ=255)                                                                                                                                                                                                           aTAAAGCAGAACAAGCACCACAATAATGGTGATTTTGATGATACGTGACATAATCCGCGCACTGGTGATATCGCGACCTTTTTTAATCTGCTGTTTTTCAAACTGATTAATCAGCAGAAATAGCTTACTCGTCAGAATAACCGCAATCAGGGACGTACAGATAAAATCGATAACGCCTGGGGTGATAAATTTGAGTTTATAGTTTTCTATAACATAATTAATAATGCTACCAACAGCACTGATAATTATgg                                      >  2:482138/1‑251 (MQ=255)                                                                                                                                                                                                                               caATCATGGTGATTTTGATGAAACGCGACATAATCCGCGCACTGGAGATATCGCGACCTTTTTTAATCTGCTGTTTTTCAAACTGATTAATCTGCAGAAATAGCTTAATCGTCCGAATAACCGCAATCAGGGACGTACAGATAAAATCGATAACGCCTGGGGTGATAAATTTGAGTTTATAGTTTTCTATAACATAATTAATAATGCTACCAACAGCACTGATAATTATGGTGTAGATTAAAAATTGCAcc                  >  2:542251/1‑251 (MQ=255)                                                                                                                                                                                                                                aataatGGTGATTTTGATGATACGTGACATAATCCGCGCACTGGTGATATCGCGACCTTTTTTAATCTGCTGTTTTTCAAACTGATTAATCAGCAGAAATAGCTTAATCGTCAGAATAACCGCAATCAGGGACGTACAGATAAAATCGATAACGCCTGGGGTGATAAATTTGAGTTTATAGTTTTCTATAACATAATTAATAATGCTACCAACAGCACTGATAATTATGGTGTAGATTAAAAAtt                       >  1:527627/1‑245 (MQ=255)                                                                                                                                                                                                                                aataatGGTGATTTTGATGATACGTGACATAATCCGCGCACTGGTGATATCGCGACCTTTTTTAATCTGCTGTTTTTCAAACTGATTAATCAGCAGAAATAGCTTAATCGTCAGAATAACCGCAATCAGGGACGTACAGATAAAATCGATAACGCCTGGGGTGATAAATTTGAGTTTATAGTTTTCTATAACATAATTAATAATGCTACCAACAGCACTGATAATTATGGTGTAGATTAAAAAtt                       <  2:527627/245‑1 (MQ=255)                                                                                                                                                                                                                                               gatgatACGTGACATAATCCGCGCACTGGTGATATCGCGACCTTTTTTAATCTGCTGTTTTTCAAACTGATTAATCAGCAGAAATAGCTTAATCGTCAGAATAACCGCAATCAGGGACGTACAGATAAAATCGATAACGCCTGGGGTGATAAATTTGAGTTTAAAGTTTTCTATAACATAATTAATAATGCTACCAACAGCACTGATAATTATGGTGTAGATTAAAAATTGCACCGCATGGAATAAAAATc  <  1:335873/251‑1 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                  GCCAGCCAATTTCCGCTACTGTACCTTCGATATTTCTGTCCGGTGAACGGATCCAGTCGCCAATACTGAAAGGACGGTCGAAATAGAGCATAATCCCGGAAAAGAAGTTACTCAGAATATCTTTACCGGCCATACCGACAGCCAGACCACCAATACCACCAAAGGTCAGCAAGCCAGAAAGGCTCATGCCGAAATGTTCGCCATAAAGCAGAACAAGCACCACAATAATGGTGATTTTGATGATACGCGACATAATCCGCGCACTGGTGATATCGCGACCTTTTTTAATCTGCTGTTTTTCAAACTGATTAATCAGCAGAAATAGCTTAATCGTCAGAATAACCGCAATCAGGGACGTACAGATAAAATCGATAACGCCTGGGGTGATAAATTTGAGTTTATAGTTTTCTATAACATAATTAATAATGCTACCAACAGCACTGATAATTATGGTGTAGATTAAAAATTGCACCGCATGGAATAAAAATC  >  NC_000913/1395319‑1395807

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑