Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,387,908	C→T	P397S (CCG→TCG)	tyrR →	DNA‑binding transcriptional dual regulator TyrR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,387,908	0	C	T	98.3%	183.6 / NA	60	P397S (CCG→TCG)	tyrR	DNA‑binding transcriptional dual regulator TyrR
Reads supporting (aligned to +/- strand):  ref base C (1/0);  major base T (34/24);  minor base G (0/1);  total (35/25)
Fisher's exact test for biased strand distribution p-value = 4.24e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTAATGATGGCACTTTCCGTCGGGTTGGCGAAGACCATGAGGTGCATGTCGATGTGCGGGTGATTTGCGCTACGCAGAAGAATCTG‑GTCGAACTGGTGCAAAAAGGCATGTTCCGTGAAGATCTCTATTATCGTCTGAACGTGTTGACGCTCAATCTGCCGCCGCTACGTGACTGTCCGCAGGACATCATGCCGTTAACTGAGCTGTTCGTCGCCCGCTTTGCCGACGAGCAGGGCGTGCCGCGTCCGAAACTGGCCGCTGACCTGAATACTGTACTTACGCGTTATGCGTGGCCGGGAAATGTGCGGCAGTTAAAGAACGCTATCTATCGCGCACTGACACAACTGGACGGTTATGAGCTGCGTCCACAGGATATTTTGTTGCCGGATTATGACGCCGCAACGGTAGCCGTGGGCGAAGATGCGATGGAAGGTTCGCTGGACGAAATCACCAGCCGTTTTGAACGCTCGGTATTAACCCAGCTTTATCGC  >  NC_000913/1387663‑1388153                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                      ttAATGATGGCACTTTCCGTCGGGTTGGCGAAGACCATGAGGTGCATGTCGATGTGCGGGTGATTTGCGCTACGCAGAAGAATCTG‑GTCGAACTGGTGCAAAAAGGCATGTTCCGTGAAGATCTCTATTATCGTCTGAACGTGTTGACGCTCAATCTGCCGCCGCTACGTGACTGTCCGCAGGACATCATGCCGTTAACTGAGCTGTTCGTCGCCCGCTTTGCCGACGAGCAGGGCGTGCCGCGTTCGaa                                                                                                                                                                                                                                                   >  1:73673/1‑250 (MQ=255)  tAATGATGGCACTTTCCGTCGGGTTGGCGAAGACCATGAGGTGCATGTCGATGTGCGGGTGATTTGCGCTACGCAGAAGAATCTG‑GTCGAACTGGTGCAAAAAGGCATGTTCCGTGAAGATCTCTATTATCGTCTGAACGTGTTGACGCTCAATCTGCCGCCGCTACGTGACTGTCCGCAGGACATCATGCCGTTAACTGAGCTGTTCGTCGCCCGCTTTGCCGACGAGCAGGGCGTGCCGCGTTCGAAAc                                                                                                                                                                                                                                                 >  1:233203/1‑251 (MQ=255)  tAATGATGGCACTTTCCGTCGGGTTGGCGAAGACCATGAGGTGCATGTCGATGTGCGGGTGATTTGCGCTACGCAGAAGAATCTG‑GTCGAACTGGTGCAAAAAGGCATGTTCCGTGAAGATCTCTATTATCGTCTGAACGTGTTGACGCTCAATCTGCCGCCGCTACGTGACTGTCCGCAGGACATCATGCCGTTAACTGAGCTGTTCGTCGCCCGCTTTGCCGACGAGCAGGGCGTGCCGCGTTCGAAAc                                                                                                                                                                                                                                                 >  2:225312/1‑251 (MQ=255)                      gggTTGGCGAAGACCATGAGGTGCATGTCGATGTGCGGGTGATTTGCGCTACGCAGAAGAATCTG‑GTAGAACTGGAGCAAAAAGGCATGTTCCGTGAAGATCTCTATTATCGTCTGAACGTGTTGACGCTCAATCTGCCGCCGCTACGTGACTGTCCGCAGGACATCATGCCGTTAACTGAGCTGTTCGTCGCCCGCTTTGCCGACGAGCAGCGCGTGCCGCGTTCGAAACTGGCCGCTGCCCTGAATACt                                                                                                                                                                                                                             >  1:295317/1‑251 (MQ=255)                      gggTTGGCGAAGACCAGGAGGTGCAGGGCGATGTGCGGGGGATTGGCGCTACGCAGAAGAATCTG‑GTCGAACTGGTGCAAAAAGGCAGGTTCCGTGAAGATCTCTATTATCGTCTGAACGTGTTGCCGCTCAATCTGCCGCCGCTACGTGACTGTCCGCAGGACATCATGCCGTTAACTGAGCTGTTCGTCGCCCGCTTTGCCGACGAGCAGGGCGTGCCGCGTTCGAAACTGGCCGCTGACCTGAATACt                                                                                                                                                                                                                             <  1:506406/251‑1 (MQ=255)                             cGAAGACCATGAGGTGCATGTCGATGTGCGGGTGATTTGCGCTACGCAGACGAATCTG‑GTCGAACTGGTGCAAAAAGGCATGTTCCGTGAAGATCTCTATTATCGTCTGAACGTGTTGACGCTCAATCTGCCGCCGCTACGTGACTGTCCGCAGGACATCATGCAGTTAACTGAGCTGTTCGTCGCCCGCTTTGCCGACGAGCATGGCGTGCCGCGTTCGAAACTGGCCGCTGACCTGTATACTGTACtt                                                                                                                                                                                                                       >  1:68855/1‑250 (MQ=255)                              gAAGACCATGAGGTGCATGTCGATGTGCGGGTGATTTACGCTACGCAGAAGACTCTG‑GTCGAACTGGTGCAAAAAGGCATGTTCCGTGAAGATCTCTATTATCGTCTGAACGTGTTGACGCTCAATCTGCCGCCGCTACGTGACTGTCCGCAGGACATCATGCCGTTAACTGAGCTGTTCGTCGCCCGCTTTGCCGACGCGCAGGGCGTGCCGCGTCCGAAACTGGCCGCTGACCTGAATACTGTACTTAc                                                                                                                                                                                                                     >  2:570879/1‑251 (MQ=255)                                  acaaggAGGTGCATGTCGATGTGCGGGTGATTGGCGCTACGCAGAAGAATCTG‑GTCGAACTGGTGCAAAAAGGAATGTTCCGTGAAGATCTCTATTATCGTCTGAACGGGTTGACGCTCAATCTGCCGCCGCTACGTGACTGTCCGCAGGACATCATGCCGTTAACTGAGCTGTTCGTCGCCCGCTTTGCCGACGAGCAGGGCGTGCCGCGTTCGAAACTGGCCGCTGACCTGAATACTGTACTTACGCGt                                                                                                                                                                                                                 <  2:11344/246‑1 (MQ=255)                                  aCCATGAGGTGCATGTCGATGTGCGGGTGATTTGCGCTACGCAGACGAATCTG‑GTCGAACTGGTGCAAAAAGGCATGTTCCGTGAAGATCTATATTATAGTCTGAACGTGTTGACGCTCAATCTGCCGCAGCTACGTGACTGTCCGCAGGACATCATGCCGTTAACTGAGCTGTTCGTCGCCCGCTTTGCCGACGAGCAGGGCGAGCCGCGTTCGAAACTGGACGCTGACCTGAATACTGTACTTAcgagt                                                                                                                                                                                                                 >  1:232524/1‑248 (MQ=255)                                     aTGAGGTGCATGTCGATGTGCGGGTGATTGGCGCTACGCAGAAGAATCTG‑GTCGAACGGGTGCAAAAAGGCATGTTCCGTGAAGATCTCTATTATCGTCGGAACGTGTTGACGCTCAATCTGCCGCCGCTACGTGACTGTCCGCAGGACATCATGCCGTTAACTGAGCTGTTCGTCGCCCGCTTTGCCGACGAGCAGGGCGTGCCGCGTTCGAAACTGGCCGCTGACCTGAATACTGTACTTACGCGTTAt                                                                                                                                                                                                              <  2:33964/251‑1 (MQ=255)                                      tGAGGTGCATGTCGATGTGAGGGTGCTTTGCGCTACGCAGAAGAATCTG‑GTCGAACTGGTGCAAAAAGGCATGTTCCGTGAAGATCTCTATTATCGTCTGAACGTGTTGACGCTCAATCTGCCGCCGCTACGTGACTGTCCGCAGGACATCATGCCGTTAACTGAGCTGTTCGTCGCCCGCTTTGCCGACGAGCAGGGCGTGCCGCGTTCGAAACTGGCCGCTGACCTGAATACTGTACTTACGCGTTATg                                                                                                                                                                                                             >  1:226186/1‑251 (MQ=255)                                              aTGTCGATGTGCGGGTGATTTGCGCTACGCAGAAGAATCTG‑GTCGAACTGGTGCAAAAAGGCATGTTCCGTGAAGATCTCTATTATCGTCTGAACGTGTTGACGCTCAATCTGCCGCCGCTACGTGACTGTCCGCAGGACATCATGCCGTTAACTGAGCTGTTCGTCGCCCGCTTTGCCGACGAGCAGGGCGTGCCGCGTTCGAAACTGGCCGCTGACCTGAATACTGTACTTACGCGTTATGCGTGGCCg                                                                                                                                                                                                     <  2:10735/251‑1 (MQ=255)                                                         ggggTGATTTGCGCTACGCAGAAGAATCTG‑GTCGAACTGGTGCAAAAAGGCATGTTCCGTGAAGATCTCTATTATCGTCTGAACGTGTTGACGCTCAATCTGCCGCCGCTACGTGACTGTCCGCAGGACATCATGCCGTTAACTGAGCTGTTCGTCGCCCGCTTTGCCGACGAGCAGGGCGTGCCGCGTTCGAAACTGGCCGCTGACCTGAATACTGTACTTACGCGTTATGCGTGGCCGGGAAATGTGCg                                                                                                                                                                                          <  1:408632/250‑1 (MQ=255)                                                           ggTGATTTGCGCTACGCAGAAGAATCTG‑GTCGAACTGGTGCAAAAAGGCATGTTCCGTGAAGATCTCTATTATCGTCTGAACGTGTTGACGCTCAATCTGCCGCCGCTACGTGACTGTCCGCAGGACATCATGCCGTTAACTGAGCTGTTCGTCGCCCGCTTTGCCGACGAGCAGGGCGTGCCGCGTTCGAAACTGGCCGCTGACCTGAATACTGTACTTACGCGTTATGCGTGGCCGGGAAATGTGCGGc                                                                                                                                                                                        >  1:564571/1‑251 (MQ=255)                                                             ggATTTGCGCTACGCAGAAGAATCGG‑GTCGAACTGGGGCAAAAAGGAATGTTCCGTGAAGATCTCTATTATCGTCTGAACGTGTTGACGCTCAATCTGCCGCCGCGACGTGACTGTCCGCAGGACATCATGCCGTTAACTGAGCTGTTCGTCGCCCGCTTTGCCGACGAGCAGGGCGTGCCGCGTTCGAAACTGGCCGCTGACCTGAATACTGTACTTACGCGTTATGCGTGGCCGGGAAATGTGCGGCAg                                                                                                                                                                                      <  1:148027/250‑1 (MQ=255)                                                                      cTACGCAGCAGAATCTG‑GTCGACCTGTTGCACAAAGGCATGTTCCGTGCAGATCTCTATTATCGTCTGAACGTGTTGACGCTCAATCTGCCGCCGCTACGTGACTGTACGCAGGACATCATGCCGTTAACTGAGCTGTTCGTCGACCGCTTTGCCGACGAGCAGGGCGTGCCGCGTTCGAAACTGGCCGCTGACCTGAATACTGTACTTACGCGTTATGCGTGGCCGGGAAATGTGCTGCCGTTAAAGAAc                                                                                                                                                                             >  2:60259/1‑251 (MQ=255)                                                                           cAGAAGAATCTG‑GTCGAACTGGTGCAAAAAGGCATGTTCCGTGAAGATCTCTATTATCGTCTGAACGTGTTGACGCTCAATCTGCCGCCGCTACGTGACTGTCCGCAGGACATCATGCCGTTAACTGAGCTGTTCGTCGCCCGCTTTGCCGACGAGCAGGGCGTGCCGCGTTCGAAACTGGCCGCTGACCTGAATACTGTACTTACGCGTTATGCGTGGCCGGGAAATGTGCGGCAGTTAAAGAACGctat                                                                                                                                                                        <  1:493273/251‑1 (MQ=255)                                                                           aataagaaTCTGTGT‑GAATTGGTGCAAAAAGGCATTTTCCTTAAAGAGCTCTATTATCTTCTGAACGTGTTGAGGCTCAGTCTGCCGCCGCTCCGTGACTGTGCGCAGGACATCATGCCGTTAACTTAGCTGTTCGTCGCCCGGTTTGCCGGCGAGCAGGGCGTGCCGCGTTCGAAACGGGCCGCTGACCTGAATACTGTACTTATGCGTTATGCGTTGCCGGGAAATGTGCGGCAGTTAAAGAACGctat                                                                                                                                                                        <  1:345004/248‑1 (MQ=255)                                                                              aagaaTCTG‑GTCGAACTGGTGCAAAAAGGCATGTTCCGTGAAGATCTCTATTATCGTCTGAACGTGTTGACGCTCAATCTGCCGCCGCTACGTGACTGTCCGCAGGACATCATGCCGTTAACTGAGCTGTTCGTCGCCCGCTTTGCCGACGAGCAGGGCGTGCCGCGTTCGAAACTGGCCGCTGACCTGAATACTGTACTTACGCGTTATGCGTGGCCGGGAAATGTGCGGCAGTTAAAGAACGctatcta                                                                                                                                                                     <  1:390922/251‑1 (MQ=255)                                                                                    cTG‑GTCGAACTGGTGCAAACAGGCATGTTCCGTGAAGATCTCTATTATCGTCTGAACGTGTTGACGCTCAATCTGCCGCCGCTACGTGACTGTCCGCAGGACATCATGCCGTTAACTGAGCTGTTCGTCGCCCGCTTTGCCGACGAGCAGGGCGTGCCGCGTTCGAAACTGGCCGCTGCCCTGAATACTGTACTTACGCGTTATGCGTGGCCGGGAAATGTGCGGCAGTTAAAGAACGCTATATATCgcgc                                                                                                                                                               >  2:515181/1‑251 (MQ=255)                                                                                          cGAACTGGTGCAAAACGGCATGTTCCGTGAAGATCTCTATTATCGTCTGCACGTGTTGACGCTCAATCTGCCGCCGCTACGTGACTGTCCGCAGGACCTCATGCCGTTAACTGAGCTGTTCGTCGCCCGCTTTGCCGACGATCAGGGCGTGCCGCGTTCGAAACTGGCCGCTGACCTGAATACTGTACTTACGCGTTATGCGTGGCCGGGAAATGTGCGGCAGTTAAAGAAAGCTATCTATCGCGCACTgc                                                                                                                                                          >  1:92848/1‑250 (MQ=255)                                                                                           gAACTGGTGCAAAAAGGCATGTTCCGTGAAGATCTTTATTATAGTCTGAACGTGTTGACGATCAATCTGCCGCCGATACGTGACTGTCCGCAGGACATCATGCCGTTAACTGAGCTGTTCGTCGCCCGCTTTGCCGACGAGCAGGGCGTGACGCGTTCGAAACTGGCCGCTGACCTGATTACTGTACTTACGCGTTATGCGTGGACGGGAATTGTGCGGCAGTTCAAGAACGCTATCTATCGCGCACTGac                                                                                                                                                         >  2:513276/1‑251 (MQ=255)                                                                                           gAACTGGTGCAAAAAGGCATGTTCCGTGAAGATCTCTATTATCGTCTGAACGTGTTGACGCTCAATCTGCCGCCGCTACGTGACTGTCCGCAGGACATCATGCCGTTAACTGAGCTGTTCGTCGCCCGCTTTGCCGACGAGCAGGGCGTGCCGCGTTCGAAACTGGCCGCTGACCTGCATACTGTACTTACGCGTTATGCGTGGCCGGGAAATGTGCGGCAGTTAAAGAACGCTATCTATCGCGCACTGac                                                                                                                                                         >  1:566067/1‑251 (MQ=255)                                                                                                     aaaaaGGCAGTTTCGGTGAAGAGCTCTATTTTCGGCTTAACGTGTTGACGCTCAATCTGCCGCCGCTACTTGACTGTCCGCAGGACATCAGGCCGTTAACTGAGCTGTTCGTCGCCCGCTTTGCCGACGAGCAGGGCGTGCCGCGTTCGAAACTGGCCGCTGACCTGAATACTGTACTTACGCGTTATGCGTGGCCGGGAAATGTGCGGCAGTTAAAGAACGCTATCTAGCGCGCACTGAGTCAACTGGAc                                                                                                                                               <  2:564571/251‑1 (MQ=255)                                                                                                       aaaGGCATGTTCCGTGAAGATCTCTATTATCGTCTGAACGTGTTGACGCTCAATCTGCCGCCGCTACGTGACTGTCCGCAGGACATCATGCCGTTAACTGAGCTGTTCGTCGCCCGCTTTGCCGACGAGCAGGGCGTGCCGCGTTCGAAACTGGCCGCTGACCTGAATACTGTACTTACGCGTTATGCGTGGCCGGGAAATGTGCGGCAGTTAAAGCACGCTATCTATCGCGCACTGACACCACTGGACgg                                                                                                                                             >  2:280507/1‑251 (MQ=255)                                                                                                       aaaGGCATGTTCCGTGAAGATCTCTATTATCGTCTGAACGTGTTGACGCTCAATCTGCCGCCGCTACGTGACTGTCCGCAGGACATCATGCCGTTAACTAAGCTGTTCGTCGCCCGCTTTGCCGACGAGCAGGGCGTGCCGCGTTCGAAACTGGCCGCTGACCTGAATACTGTACTTACGCGTTATGCGTGGCCGGGAAATGTGCGGCAGTTAAAGAACGCTATCTATCGCGCAATGACACAACTGGACgg                                                                                                                                             >  2:420043/1‑251 (MQ=255)                                                                                                        aaGGCATGTTCCGTGAAGATCTCTATTATCGTCTGAACGTGTTGACGCTCAATCTGCCGCCGCTACGTGACTGTCCGCAGGACATCCTGCCGTTAACTGAGATGTTCGTCGCCCGCTTTGCCGACGAGCATGGCGTGCCGCGTTCGAAACTGGCCGCTGACCTGAATACTGTACTTACGCGTTATGCGTGGCCGGGAAATGTGCGGCAGTTAAAGAACGCTATCTATCGCGCACTGACACAACTGGACGGt                                                                                                                                            >  1:540407/1‑251 (MQ=255)                                                                                                           gCATGTTCCGTGAAGATCTCAATTATCGTCTGAACGTGTTGACGCTCAATCTGCCGCCGCTACGTGACTGTCCGCAGGACATCATGCCGTTAACTGAGCTGTTCGTCGCCAGCTTTGCCGACGAGCAGGGCGTGCCGCGTTCGAAACTGGCCGCTGACCTGAATACTGTACTTACGCGTTATGCGTGGCCGGGAAATGTGCGGCAGTTAAAGAACGCTATCTATCGCGCACTGACACACCTGGACGGTTAt                                                                                                                                         >  2:500055/1‑251 (MQ=255)                                                                                                               gTTCCGTGAAGATCTCTATTATCGTCTGAACGTGTTGACGCTCAATCTGCCGCCGCTACGTGACTGTCCGCAGGACATCATGCCGTTAACTGAGCTGTTCGTCGCCCGCTTTGCCGACGAGCAGGGCGTGCCGCGTTCGAAACTGGCCGCTGACCTGAATACTGTACTTACGCGTTATGCGTGGCCGGGAAATGTGCGGCAGTTAAAGAACGCTATCTATCGCGCACTGACACAACTGGACGGTTATGAGc                                                                                                                                     <  1:39597/251‑1 (MQ=255)                                                                                                               gTTCCGTGAAGATCTCTATTATCGTCTGAACGTGTTGACGCTCAATCTGCCGCCGCTACGTGACTGTCCGCAGGACATCATGCCGTTAACTGAGCTGTTCGTCGCCCGCTTTGCCGACGAGCAGGGCGTGCCGCGTTCGAAACTGGCCGCTGACCTGAATACTGTACTTACGCGTTATGCGTGGCCGGGAAATGTGCGGCAGTTAAAGAACGCTATCTATCGCGCACTGACACAACTGGACGGTTATGAGc                                                                                                                                     >  2:477336/1‑251 (MQ=255)                                                                                                                       aaGATCTCTATTATCGTCTGAACGTGTTGACGCTCAATCTGCCGCCGCTACGTGACTGTCCGCAGGACATCATGCCGTTAACTGAGCTGTTCGTCGCCCGCTTTGCCGACGAGCAGGGCGTGCCGCGTTCGAAACTGGCCGCTGACCTGAATACTGTACTTACGCGTTATGCGTGGCCGGGAAATGTGCGGCAGTTAAAGAACGCTATCTATCGCGCACTGACACAACTGGACGGTTATGAGCTGCGTCca                                                                                                                             <  1:119851/251‑1 (MQ=255)                                                                                                                                attaaCGTCTGAACGTGTTGACGCGCAATCTGCCGCCGCTTCGTGACTGTCCGCAGGACATCATGCCGTTAACTGAGCTGTTCGTCGCCCGCTTTGCCGACGAGCAGGGCGTGCCGCGTTCGAAACTGGCCGCTGACCTGAATACTGTACTTACGCGTTATGCGTGGCCGGGAAATGTGCGGCAGTTAAAGAACGCTATCTATCGCGCACTGACACAACTGGACGGTTATGAGCTGCGTCCACAGGatat                                                                                                                     <  1:5560/250‑1 (MQ=255)                                                                                                                                      gTCTGAACGTGTTGACGCTCAATCTGCCGCCGCTACGTGACTGTCCGCAGGACATCATGCCGTTAACTGAGCTGTTCGTCGCCCGCTTTGCCGACGAGCAGGGCGTGCCGCGTTCGAAACTGGCCGCTGACCTGAATACTGTACTTACGCGTTATGCGTGGCCGGGAAATGTGCGGCAGTTAAAGAACGCTATCTATCGCGCACTGACACAACTGGACGGTTATGAGCTGCGTCCACAGGATATTttgttg                                                                                                              >  2:335733/1‑251 (MQ=255)                                                                                                                                         tGAACGTGTTGACGCTCAATCTGCCGCCGCTACGTGACTGTCCGCAGGACATCATGCCGTTAACTGAGCTGTTCGTCGCCCGCTTTGCCGACGAGCAGGGCGTGCCGCGTTCGAAACTGGCCGCTGACCTGAATACTGTACTTACGCGTTATGCGTGGCCGGGAAATGTGCGGCAGTTAAAGAACGCTATCTATCGCGCACTGACACAACTGGACGGTTATGAGCTGCGTCCACAGGATATTTTGTTGCCg                                                                                                           >  1:346955/1‑251 (MQ=255)                                                                                                                                           aaCGTGTTGACGCTCAATCTGCCGCCGCTACGTGACTGTCCGCAGGACATCATGCCGTTAACTGAGCTGTTCGTCGCCCGCTTTGCCGACGAGCAGGGCGTGCCGCGTTCGAAACTGGCCGCTGACCTGAATACTGTACTTACGCGTTATGCGTGGCCGGGAAATGTGCGGCAGTTAAAGAACGCTATCTATCGCGCACTGACACAACTGGACGGTTATGAGCTGCGTCCACAGGATATTTTGTTGCCgg                                                                                                          >  2:347459/1‑250 (MQ=255)                                                                                                                                                gtTGACGCTCAATCTGCCGCCGCTACGTGACTGTCCGCAGGACATCATGCCGTTAACTGAGCTGTTCGTCGCCCGCTTTGCCGACGAGCAGGGCGTGCCGCGTTCGAAACTGGCCGCTGACCTGAATACTGTACTTACGCGTTATGCGTGGCCGGGAAATGTGCGGCAGTTAAAGAACGCTATCTATCGCGCACTGACACAACTGGACGGTTATGAGCTGCGTCCACAGGATATTTTGTTGCCGGATTATg                                                                                                    <  1:359965/251‑1 (MQ=255)                                                                                                                                                          aaTCTGCCGCCGCTACGTGACTGTCCGCAGGACATCATGCCGTTAACTGAGCTGTTCGTCGCCCGCTTTGCCGACGAGCAGGGCGTGCCGCGTTCGAAACTGGCCGCTGACCTGAATACTGTACTTACGCGTTATGCGTGGCCGGGAAATGTGCGGCAGTTAAAGAACGCTATCTATCGCGCACTGACACAACTGGACGGTTATGAGCTGCGTCCACAGGATATTTTGTTGCCGGATTATGACGCCGCAAc                                                                                          >  1:177371/1‑251 (MQ=255)                                                                                                                                                          aaTCTGCCGCCGCTACGTGACTGTCCGCAGGACATCATGCCGTTAACTGAGCTGTTCGTCGCCCGCTTTGCCGACGAGCAGGGCGTGCCGCGTTCGAAACTGGCCGCTGACCTGAATACTGTACTTACGCGTTATGCGTGGCCGGGAAATGTGCGGCAGTTAAAGAACGCTATCTATCGCGCACTGACACAACTGGACGGTTATGAGCTGCGTCCACAGGATATTTTGTTGCCGGATTATGACGCCGCAAc                                                                                          >  1:565439/1‑251 (MQ=255)                                                                                                                                                            tCTGCCGCCGCTACGTGACTGTCCGCAGGACATCATGCCGTTAACTGAGCTGTTCGTCGCCCGCTTTGCCGACGAGCAGGGCGTGCCGCGTTCGAAACTGGCCGCTGACCTGAATACTGTACTTACGCGTTATGCGTGGCCGGGAAATGTGCGGCAGTTAAAGAACGCTATCTATCGCGCACTGACACAACTGGACGGTTATGAGCTGCGTCCACAGGATATTTTGTTGCCGGATTATGAAGCCGCCACgg                                                                                        >  2:19700/1‑251 (MQ=255)                                                                                                                                                               gccgccGCTACGTGACTGTCCGCAGGACATCATGCCGTTAACTGAGCTGTTCGTCGCCCGCTTTGCCGACGAGCAGGGCGTGCCGCGTTCGAAACTGGCCGCTGACCTGAATACTGTACTTACGCGTTATGCGTGGCCGGGAAATGTGCGGCAGTTAAAGAACGCTATCTATCGCGCACTGACACAACTGGACGGTTATGAGCTGCGTCCACAGGATATTTTGTTGCCGGATTATGACGCCGCAACg                                                                                         <  1:346988/247‑1 (MQ=255)                                                                                                                                                                       gaCGTGACTGTCCGCAGGACATCATGCCGTTAACTGAGCTGTTCGTCGCCCGCTTTGCCGACGAGCAGGGCGTGCCGCGTTCGAAACTGGCCGCGGACCTGAGTACTGTACTTACGCGTTATGCGTGGCCGGGAAATGTGCGGCAGTTAAAGAACGCTATCTATCGCGCACTGATACAACTGGACGGTTATGAGCTGCGTCCACAGGATATTTTGTTGCCGGATTATGACGCCGCAACGGTAGCCGTGGGc                                                                             <  1:339829/250‑1 (MQ=255)                                                                                                                                                                        aCGTGACTGTCCGCAGGACATCATGCCGTTAACTGAGCTGTTCGTCGCCCGCTTTGCCGCCGAGCAGGGCGTGCCGCGTTCGAAACTGGCCGCTGACCTGAATACTGTACTTACGCGTTATGCGTGGCCGGGAAATGTGCGGCAGTTAAAGAACGCTATCTATCGCGCACTGACACAACTGGACGGTTATGAGCTGCGTCCACAGGATATTTTGTTGCCGGATTATGACGCCGCAACGGTAGCCGTGGGCg                                                                            >  2:578246/1‑251 (MQ=255)                                                                                                                                                                           tGACTGTCCGCAGGACATCATGCCGTTAACTGAGCTGTTCGTCGCCCGCTTTGCCGACGAGCAGGGCGTGCCGCGTTCGAAACTGGCCGCTGACCTGAATACTGTACTTACGCGTTATGCGTGGCCGGGAAATGTGCGGCAGTTAAAGAACGCTATCTATCGCGCACTGACACAACTGGACGGTTATGAGCTGCGTCCACAGGATATTTTGTTGCCGGATTATGACGCCGCAACGGTAGCCGTGGGCGa                                                                           >  1:16634/1‑249 (MQ=255)                                                                                                                                                                            gACTGTCCGCAGGACATCATGCCGTTAACTGAGCTGTTCGTCGCCCGCTTTGCCGACGAGCAGGGCGTGCCGCGTTCGAAACTGGCCGCTGACCTGAATACTGTACTTACGCGTTATGCGTGGCCGGGAAATGTGCGGCAGTTAAAGAACGCTATCTATCGCGCACTGACACAACTGGACGGTTATGAGCt                                                                                                                                    >  1:208410/1‑191 (MQ=255)                                                                                                                                                                            gACTGGCCGCAGGACATCATGCCGTTAACTGAGCTGTTCGTCGCCCGCTTTGCCGACGAGCAGGGCGTGCCGCGTTCGAAACTGGCCGCTGACCTGAATACTGTACTTACGCGTTATGCGTGGCCGGGAAATGGGCGGCAGTTAAAGAACGCTATCTATCGCGCACTGACACAACTGGACGGTTATGAGCt                                                                                                                                    <  2:208410/191‑1 (MQ=255)                                                                                                                                                                                     cAGGACATCCTGCCGTTAACTGAGCTATTCGTCGCCCGCTTTGCCGACGAGCAGGGCGTGCCGCGTTCGAAACTGGCCGCTGACCTGAATACTGTACTTACGCGTTCTGCGTGGCCGGGAAATGTGCGGAAGATAAAGAACGCTATCTATCGCGCACTGACACAACTGGACGGTTATGAGCTGCGTCCACAGGATATTTTGTTGCCGGATTATGACGCCGCAACGGTAGCCGTGGGCGAAGATGCGATgg                                                                >  2:270206/1‑250 (MQ=255)                                                                                                                                                                                      gggccatcatGCCGTTAACTTAGCTGTTCGTCGCCCGCTTTGCCGACGAGCAGTGAGTGCCGCGTTCGAAACTGGCCGCTTACTTGAATACTGTACTTACGCGTTATGCGTGGCCGGGAAATGTGCGGCAGTTAAAGAACGCTATCTATCGCGCACTGACACATCTTGACGGTTATGAGCTGCGGCCACAGGATATGTTGTTGCCGGATTATGACGGCGCAACGGTAGCGGTGGTCGAAGATGCGATGGaa                                                              <  2:408164/247‑1 (MQ=255)                                                                                                                                                                                           atcatGACGTTAACTGAGCTGTTCGTCGCCCGCTTTGCCGACGAGCAGGGCGTGCCGCGTTCGAAACTGGCCGCTGACCTGAATACTGTACTTACGCGTTATGCGTGGCCGGGAAATGTGCGGCAGTTAAAGAACGCTATCTATCGCGCACTGACACAACTGGACGGTTATGAGCTGCGTCCACAGGATATTTTGTTGCCGGATTATGACGCCGCAACGGTAGCCGTGGGCGAAGATGCGATGGAAGGTTc                                                         <  1:390469/251‑1 (MQ=255)                                                                                                                                                                                              atGCCGTTAACTGAGCTGTTCGTCGCCCGCTTTGCCGACGAGCAGGGCGTGCCGCGTTCGAAACTGGCCGCTGACCTGAATACTGTACTTACGCGTTATGCGTGGCCGGGAAATGTGCGGCAGTTAAAGAACGCTATCTATCGCGCACTGACACAACTGGACGGTTATGAGCTGCGTCCACAGGATATTTTGTTGCCGGATTATGACGCCGCAACGGTAGCCGTGGGCGAAGATGCGATGGAAGGTTCGCt                                                      >  2:145352/1‑251 (MQ=255)                                                                                                                                                                                                   gTTAACTGAGCTGTTCGTCGCCCGCTTTGCCGACGAGCAGGGCGTGCCGCGTTCGAAACTGGCCGCTGACCTGAATACTGTACTTACGCGTTATGCGTGGCCGGGAAATGTGCGGCAGTTAAAGAACGCTATCTATCGCGCACTGACACAACTGGACGGTTATGAGCTGCGTCCACAGGATATTTTGTTGCCGGATTATGACGCCGCAACGGTAGCCGTGGGCGAAGATGCGATGGAAGGTTCGCTGGACg                                                 >  1:202826/1‑251 (MQ=255)                                                                                                                                                                                                                       cccGCTTTGCCGACGAGCAGGGCGTGCCGCGTTCGAAACTGGCCGCTGACCTGAATACTGTACTTACGCGTTATGCGTGGCCGGGAAATGTGCGGCAGTTAAAGAACGCTATCTATCGCGCACTGACACAACTGGACGGTTATGAGCTGCGTCCACAGGATATTTTGTTGCCGGATTATGACGCCGCAACGGTAGCCGTGGGCGAAGATGCGATGGAAGGTTCGCTGGACGAAATCACCAGCCGTTTTGaa                             <  1:500055/251‑1 (MQ=255)                                                                                                                                                                                                                       cccGCTTTGCCGACGAGCAGGGCGTGCCGCGTTCGAAACTGGCCGCTGACCTGAATACTGTACTTACGCGTTATGCGTGGCCGGGAAATGTGCGGCAGGTAAAGAACGCTATCTATCGCGCACTGACACAACTGGACGGTTATGAGCTGCGTCCACAGGATATTTTGTTGCCGGATTATGACGCCGCAACGGTAGCCGTGGGCGAAGATGCGATGGAAGGTTCGCTGGACGAAATCACCAGCCGTTTTGaa                             <  2:199320/251‑1 (MQ=255)                                                                                                                                                                                                                             ttGCCGACGAGCAGGGCGTGCCGCGTTCGAAACTGGCCGCTGACCTGAATACTGTACTTACGCGTTATGCGTGGCCGGGAAATGTGCGGCAGTTAAAGAACGCTATCTATCGCGCACTGACACAACTGGACGGTTATGAGCTGCGTCCACAGGATATTTTGTTGCCGGATTATGACGCCGCAACGGTAGCCGTGGGCGAAGATGCGATGGAAGGTTCGCTGGACGAAATCACCAGCCGTTTTGAACGCTCg                       >  1:14436/1‑251 (MQ=255)                                                                                                                                                                                                                                 cgacgaGCAGGGAGAGCCGCGTTCGAAACTGGCCGCTGACCTGAATACTGTACTTACGCGTTATGCGTGGCCGGGAAATGTGCGGCCGTTAAAGAACGCTATCTATCGCGCACTGACACAACTGGACGGTTATGAGCTGCGTCCACAGGATATTTTGTTGCCGGATTATGACGCCGCAACGGTAGCCGTGGGCGAAGATGCGATGGACGGTTCGCTGGACGAAATCACCAGCCGTTTTGAACGCTCGGTAt                   >  2:55508/1‑251 (MQ=255)                                                                                                                                                                                                                                      cgCAGGGCGTGCCGCTTGCGAAACGTGCCGCTGCCCTTAATACTGTACTTACGCGTTATGCGTGGCCGGCAAATGTGCGTCAGTTAAAGAACTCTTTCTAGCGCTCACTGACGCAACTGGACGGTTATGAGCTGCTGCCACAGGATATTTTGTTGCCTGATTATGACGCCGCAACGGTAGCCGTGGGCGAATATGCTATGGAAGGTTCGCTGGACGAAATCACCAGCCGTTTTTAACGCTCGGTATTAAcc              <  2:540407/250‑1 (MQ=255)                                                                                                                                                                                                                                        cAGGGCGTGCCGCGTTCGAAACTGGCCGCTGACCTGAATACTGTACTTACGCGTTATGCGTGGCCGGGAAATGTGCGGCAGTTAAAGAACGCTATCTATCGCGCACTGCCACAACTGGACGGTTATGACCTGCGTCCACAGGATATTTTGTTGCCGGATTATGACGCCGCAACGGTAGCCGTGGGCGACGATGCGATGGAAGGTTCGCTGGACGAAATCACCAGCCGTTTTGAACGCTCGGTATTAcccc             >  1:343283/1‑250 (MQ=255)                                                                                                                                                                                                                                             cGTGCCGCGTTCGAAACTGGCCGCTGACCTGAATACTGTACTTACGCGTTATGCGTGGCCGGTAAATGTGCGGCAGTTAAAGAACGCTATCTATCGCGCACTGACACAACTGGACGGTTATGAGCTGCGTCCACAGGATATTTTGTTGCCGGATTATGACGCCGCAACGGTAGCCGTGGGCGAAGATGCGATGGAAGGTTCGCTGGACGAAATCACCAGTCGTTTTGAACGCTCGGTATTAACCCAGCttt       <  2:73673/251‑1 (MQ=255)                                                                                                                                                                                                                                              gTGCCGCGTTCGAAACTGGCCGCTGACCTGAATACTGTACTTACGCGTTATGCGTGGCCGGGAAATGTGCGGCAGTTAAAGAACGCTATCTATCGCGCACTGACACAACTGGACGGTTATGAGCTGCGTCCACAGGATATTTTGTTGCCGGATTATGACGCCGCAACGGTAGCCGTGGGCGAAGATGCGATGGAAGGTTCGCTGGACGAAATCACCAGCCGTTTTGAACGCTCGGTATTAACCCAGCTTTa      <  1:279855/251‑1 (MQ=255)                                                                                                                                                                                                                                                  cgcgTTCGAAACTGGCCGCTGACCTGAATACTGTACTTACGCGTTATGCGTGGCCGGGAAATGTGCGGCAGTTAAAGAACGCTATCTATCGCGCACTGACACAACTGGACGGTTATGAGCTGCGTCCACAGGATATTTTGTTGCCGGATTATGACGCCGCAACGGTAGCCGTGGGCGAAGATGCGATGGAAGGTTCGCTGGACGAAATCACCAGCCGTTTTGAACGCTCGGTATTAACCCAGCTTTATCGc  <  2:11526/251‑1 (MQ=255)                                                                                                                                                                                                                                                   gcgTTCGAAACTGGCCGCTGACCTGAATACTGTACTTACGCGTTATGCGTGGCCGGGAAATGTGCGGCAGTTAAAGAACGCTATCTATCGCGCACTGACACAACTGGACGGTTATGAGCTGCGTCCACAGGATATTTTGTTGCCGGATTATGACGCCGCAACGGTAGCCGTGGGCGAAGATGCGATGGAAGGTTCGCTGGACGAAATCACCAGCCGTTTTGAACGCTCGGTATTAACCCAGCTTTATCGc  >  2:457919/1‑250 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                      TTAATGATGGCACTTTCCGTCGGGTTGGCGAAGACCATGAGGTGCATGTCGATGTGCGGGTGATTTGCGCTACGCAGAAGAATCTG‑GTCGAACTGGTGCAAAAAGGCATGTTCCGTGAAGATCTCTATTATCGTCTGAACGTGTTGACGCTCAATCTGCCGCCGCTACGTGACTGTCCGCAGGACATCATGCCGTTAACTGAGCTGTTCGTCGCCCGCTTTGCCGACGAGCAGGGCGTGCCGCGTCCGAAACTGGCCGCTGACCTGAATACTGTACTTACGCGTTATGCGTGGCCGGGAAATGTGCGGCAGTTAAAGAACGCTATCTATCGCGCACTGACACAACTGGACGGTTATGAGCTGCGTCCACAGGATATTTTGTTGCCGGATTATGACGCCGCAACGGTAGCCGTGGGCGAAGATGCGATGGAAGGTTCGCTGGACGAAATCACCAGCCGTTTTGAACGCTCGGTATTAACCCAGCTTTATCGC  >  NC_000913/1387663‑1388153

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑