| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,335,123 | C→T | intergenic (+294/‑25) | cysB → / → ymiA | DNA‑binding transcriptional dual regulator CysB/uncharacterized protein YmiA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,335,123 | 0 | C | T | 96.4% | 90.1 / NA | 28 | intergenic (+294/‑25) | cysB/ymiA | DNA‑binding transcriptional dual regulator CysB/uncharacterized protein YmiA |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (15/12); minor base G (0/1); total (15/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.64e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TTTGTTACTTATTTCTATACCGTGCACAATATTTTTATCCCTCATATTTATAGGGTAAATTACCTGTAAAGTAGTGTCTAATTACCCGCCTCGCAAACTCCAGTTTTCAAATATCTATTTCAGGTCAAAAGATTGAATTATTCACTGGAGACGATTCGTAAATTCGCTGG‑ATTTTTGACTAAAGTTTCTTTAGGATTTGTCTCATCGTATGATTAATTACACCAATCGTTCAACGTCTTGCTGATAAGTGATGGCGATTATATGAGGTTAGCAATGCCTTCTGGAAATCAGGAACCCCGCCGCGATCCTGAATTAAAACGTAAAGCCTGGCTGGCGGTTTTTCTTGGTTCTGCACTTTTCTGGGTGGTTGTCGCACTGCTGATTTGGAAAGTGTGGGGATAACTATGGTCGGTCAGGAGCAACTGGAGTCGTCACCATTATGCCAGCATAGTGACAATGAAACAGAAACGAAACGGGAATGTT > NC_000913/1334887‑1335369 | tttGTTACTTATTTCTATACCGTGCACAATATTTTTATCCCTCATATTTATAGGGTAAATTACCTGTAAAGTAGTGTCTAATTACCCGCCTCGCAAACTCCAGTTTTCAAATATCTATTTCAGGTCAAAAGATTGAATTATTCACTGGAGACGATTCGTAAATTCGCTGG‑ATTTTTGACTAAAGGTTCTTTAGGATTTGTCTCATCGTATGATTAATTACACCAATCGTTCAACGTTTTGCTGATAAGt < 1:63425/249‑1 (MQ=255) cGTGCACAATATTTTTATCCCTCATATTTATAGGGTAAATTAACTGTAAAGTAGTGTCTAATTACCCGCCTCGCAAACTCCAGTTTTCAAATATATATTTCAGGTCAAAAGATTGAATTATTCACTGGAGACGATTCGTAAATTCGCTGA‑ATTTTTGACTAAAGTTTCTTTAGGATTTGTCTCATCGTATGATTAATTACACCAATCGTTCAACGTTTTGCTGATAAGTGATGGAGATTATATGAGGtt > 1:516528/1‑249 (MQ=255) aacaATATTTTTATCCCTCATATTTATAGGGTAAATTACCGGTAAAGTAGTTTCTAATTACCCGCCTCGCAAACTCCAGTTTTCAAATATCTATTTCAGGTCAAAAGATTGAATTATTCACTGGAGACGATTCGTAAATTCGCGGG‑ATGTTTGACTAAAGTTGCTTTAGGATTTGTCTCAGCGTATGATTAATTACACCAAGCGATCAAAGTTTTGCGGATAAGTGATGGCGATTATATGATGTTAGCaag < 2:94672/250‑2 (MQ=255) atTTTTAGCCCTAATATTTATAGGGTAAATTTCCTTTAAAGTAGGGTCTAATTACCCGCCTCGCAAACTCCAGTTTTCAAATATCTATTTCAGGTCAAAAGATTGAATTATTCTCTGGAGACGATTCGTAAATTCGCTGG‑ATTTTTGACTAAAGTTTCTTTAGGATTTGGCTCATCGTATGATTAATTACACCAATCGTTCAACGTTTTGCTGAGAAGTGATGGCTATAAGATGAGGTTAGCAATGCCtt < 2:30109/250‑1 (MQ=255) tatTTATAGGGTAAATTACCTGTAAAGTAGTGTCTAATTACCCGCCTCGCAAACTCCAGTTTTCAAATATCTATTTCAGGTCACAAGATTGAATTATTCACTGGAGCCGATTCGTAAATTCGCTGG‑ATTTTTGACTAAAGTTTCTTTAGGATTTGTCTCATCGTATGATTAATTACACCAATCGTTCAACGTTTTGCTGATAAGTGATGGCGATTATATGAGGTTAGCAATGCCTTCTGGAAATCAGGAAc > 1:504037/1‑251 (MQ=255) atTTATAGGGTAAATTACCTGTAAAGTAGTGTCTAATTACCCGCCTCGCAAACTCCAGTTTTCAAATATCTATTTCAGGTCAAAAGATTGAATTATTCACTGGAGACGATTCGTAAATTCGCTGG‑ATTTTTGACTAAAGTTTCTTTAGGATTTGTCTCATCGTATGATTAATTACACCAATCGTTCAACGTTTTGCTGATAAGTGATGGCGATTATATGAGGTTAGCAATGCCTTCTGGAAATCAGGAAcc > 1:179634/1‑251 (MQ=255) tACCTGTAAAGTAGTGTCTAATTACCCGCCTCGCAAACTCCAGTTTTCAAATATCTATTTCAGGTCAAAAGATTGAATTATTCACTGGAGACGATTCGTAAATTCGCTGG‑ATTTTTGACTAAAGTTTCTTTAGGATTTGTCTCATCGTATGATTAATTACACCAATCGTTCAACGTTTTGCTGATAAGTGATGGCGATTATATGAGGTTAGCAATGCCTTCTGGAAATCAGGAACCCCGCCGCGATCCTg > 2:81798/1‑250 (MQ=255) gtaagtgattgtatAATTACCCGCCTCGCAAACTCCAGTTTTCAAATATCTATTTCAGGTCAAAAGTTTGAATTATTCTCTGGAGACGATTCGTAAATTCGCTGG‑ATGTTTGACTAAAGTTTCTTTAGGATTTTTCTCATCGTATGATTAATTACACCAATCGTTCAACGTTTTGCGGAAAAGTGATGGCGATTATATGAGGTTAGAAATGCCTTCTGAAAATCAGAAACCCCGCCGCGATCCTGAATTaa < 2:322609/238‑1 (MQ=255) tAATTACCCGCCTCGCAAACTCCAGTTTTCAAATATCTATTTCAGGTCAAAAGATTGAATTATTCACGGGAGACGATTCGTAAATTCGCTGG‑ATTTTTGACTAAAGTTTCTTTAGGATTTGTCTCATCGTATGATTAATTACACCAATCGTTCAACGTTTTGCGGATAAGTGATGGCGATTATATGAGGTTAGCAAGGCCTTCTGGAAATCAGGAACCCCGCCGCGATCCTGAATTAAAACGTAAAGCct < 1:81798/250‑1 (MQ=255) tACCCGCCTCGCAAACTCCAGTTTTCAAATATCTATTTCAGGTCAAAAGATTGAATTATTCACTGGAGACGATTCGTAAATTCGCTGG‑ATTTTTGACTAAAGTTTCTTTAGGATTTGTCTCATCGTATGATTAATTACACCAATCGTTCAACGTTTTGCTGATAAGTGATGGCGATTATATGAGGTTAGCAATGCCTTCTGGAAATCAGGAACCCCGCCGCGATCCTGAATTAAAACGTAAAGCctggctg > 2:127930/1‑251 (MQ=255) ccTCGCAAACTCCAGTTTTCAAATATCTATTTCAGGTCAAAAGATTGAATTATTCACTGGAGACGATTCGTAAATTCGCTGG‑ATTTTTGACTAAAGTTTCTTTAGGATTTGTCTCATCGTATGATTAATTACACCACTCGTTCAACGTTTTGCTGATAAGTGATGGCGATTatat > 2:485271/1‑175 (MQ=255) ccTCGCAAACTCCAGTTTTCAAATATCTATTTCAGGTCAAAAGATTGAATTATTCACTGGAGACGATTCGTAAATTCGCTGG‑ATTTTTGACTAAAGTTTCTTTAGGATTTGTCTCATCGTATGATTAATTACACCAATCGTTCAACGTTTTGCTGATAAGTGATGGCGATTatat < 1:485271/175‑1 (MQ=255) cAAACTCCAGTTTTCAAATATCTATTTCAGGTCAAAAGATTGAATTATTCACTGGAGACGATTCGTAAATTCGCTGG‑ATTTTTGACTAAAGTTTCTTTAGGATTTGTCTCATCGTATGATTAATTACAACAATCGATCAACGTTTTGCTGATAAGTGATGGCGATTATATGAGGTTAGCAATGCCTTATGGAAATCAGGAACCCCGCCGCGATCCTGAATTAAAACGTAAAGCCTGGCTGGCGGTTTTTCt > 1:356953/1‑251 (MQ=255) atCTATTTCAGGTCAAAAGATTGAATTATTCACTGGAGACGATTCGTAAATTCGCTGG‑ATTTTTGACTAAAGTTTCTTTAGGATTTGTCTCATCGTATGATTAATTACACCAATCGTTCAACGTTTTGCTGATAAGTGATGGCGATTATATGAGGTTAGCAATGCCTTCTGGAAATCAGGAACCCCGCCGCGATCCTGAATTAAAACGTAAAGCCTGGCTGGCGGTTTTTCTTGGTTCTGCACTTTTCTgg > 1:132934/1‑251 (MQ=255) atCTATTTCAGGTCAAAAGATTGAATTATTCACTGGAGACGATTCGTAAATTCGCTGG‑ATTTTTGACTAAAGTTTCTTTAGGATTTGTCTCATCGTATGATTAATTACACCAATCGTTCAACGTTTTGCTGATAAGTGATGGCGATTATATGAGGTTAGCAATGCCTTCTGGAAATCAGGAACCCCGCCGCGATCCTGAATTAAAACGTAAAGCCTGGCTGGCGGTTTTTCTTGGTGCTGCACTTTTCTgg > 2:7203/1‑251 (MQ=255) aTTTCAGGTCAAAAGATTGAATTATTCACTGGAGACGATTCGTAAATTCGCTGG‑ATTTTTGACTAAAGTTTCTTTAGGATTTGTCTCATCGTATGATTAATTACACCAATCGTTCAACGTTTTGCTGATAAGTGATGGCGATTATATGAGGTTAGCAATGCCTTCTGGAAATCAGGAACCCCGCCGCGATCCTGAATTAAAACGCAAAGCCTGGCTGGCGGCTTTTCTTGGTTCTGCACTTTTATggtcgg > 1:258807/1‑247 (MQ=255) cAAAAGATTGAATTATTCACTGGTGACGATTCGTAAATTCGCTGG‑ATTTTTGACTAAAGTTTATTTAGGATTTGTCTCATCGTATGATTAATTACACCAATCGTTCAACGTTTTGCTGATAAGTGATGGCGATTATATGAGGTTAGCAATGCCTTCTGGAAATCAGGAACACCGCCGCGATCCTGAATTAAAACGTAAAGCCTGGCTGGCGGTTTTTCTTGGTTCTGCACTTTTCTGGGGGGTTGTCGCAc > 2:218678/1‑251 (MQ=255) gAATTATTCATTGGAGACGATTAGTAAATTCG‑TGGTATTTTTGATTAAAGTTTCTTTAGGATTTGTCGTATGGTTGGATGAATTACTCCTATCGTTCAAGGTGTTGCGAATAAGTGAGGGCGATTATATGAGGTGCGCACTGCCTTCTGGAAACCAGGAGCTCTGCTGCGATCCTGAATGAATTCGTAAAGCCTGGCTGGCGTTTTTGCTTGGTTCTGCCCTTTTCTGGGTGGTTGTCGCACTGCTGAttt < 1:38187/251‑1 (MQ=255) attattCACTGGAGACGATTCGTAAATTCGCTGG‑ATTTTTGACTAAAGTTTCTTTAGGATTTGTCTCATCGTATGATTAATTACACCAATCGTTCAACGTTTTGCTGATAAGTGATGGCGATTATATGAGGTTAGCAATGCCTTCTGGAAATCAGGACCCCCGCCGCGATCCTGAATTAAAACGTAAAGCCTGGCTGGCGGTTTTTCTTGGTTCTGCACTTTTCTGGGTGGTTGTCGCACTGCTGATTTgg < 2:225015/251‑1 (MQ=255) ttattCACTGGAGACGATTCGTAAATTCGCTGG‑ATTTTTGACTAAAGTTTCTTTAGGATTTGTCTCATCGTATGATTAATTACACCAATCGTTCAACGTTTTGCTGATAAGTGATGGCGATTATATGAGGTTAGCAATGCCTTCTGGAAATCAGGAACCCCGCCGCGATCCTGAATTAAAACGTAAAGCCTGGCTGGCGGTTTTTCTTGGTTCTGCACTTTTCTGGGTGGTTGTCGCACTGCTGATTTggg > 1:531088/1‑250 (MQ=255) aCTGGAGACGATTCGTAAATTCGTTGG‑ATTTTTGACTAAAGTTTCTTTAGGATTTGTCTAATCGTATGATTAATTACACCAATCGTTCACCGTTTTGCTGATAAGTGATGGCGATTATATGAGGTTAGCAATGCCTTCTTGAAATCAGGAACCCCGCCGCGATCCTGAATTAAAACGTAAAGCCTGGCTGGCGGTTTTTCTTGGTTCTGCACTTTTCTGGGTGGTTGTCGCACTGCTGATTTGGAAAgtgt < 1:568424/251‑1 (MQ=255) cGATTCGTAAATTCGCTGG‑ATTTTTGACTAAAGTTTCTTTAGGCTTTGTCTCATCGTATGATTAATTACACCAATAGCTCAAAGTTTTGCTGATAAGTGATCGAGATTATATGCGGTTAGCAATGCCTTCTGGAAATCAGGCACCCCGCCGCGCTCCTGAATTAAAACGTAAAGCATGGCTGGCGGTTTTTCTTGGTTCTGCCCTTTTTTGGGCGGTTGTCGCACTGCTAATTTGTAAAGTGTggggcta > 1:534472/1‑247 (MQ=255) cGTAAATTCGCTGG‑ATTTTTGACTAAAGTTTCTTTAGGATTTGTCTCATCGTATGATTAATTACACCAATCGTTCAACGTTTTGCTGATAAGTGATGGCGATTATATGAGGTTAGCAATGCCTTCTGGAAATCAGGAACCCCGCCGCGATcc > 2:121912/1‑152 (MQ=255) cGTAAATTCGCTGG‑ATTTTTGACTAAAGTTTCTTTAGGATTTGTCTCATCGTATGATTAATTACACCAATCGTTCAACGTTTTGCTGATAAGTGATGGCGATTATATGAGGTTAGCAATGCCTTCTGGAAATCAGGAACCCCGCCGCGATcc < 1:121912/152‑1 (MQ=255) attaattaCACCAATCGTTCAACGTTTTGCTGATAAGTGATGGCGATTATATGAGGTTAGCAATGCCTTCTGGAAATCAGGAACCCCGCCGCGATCCTGAATTAAAACGTAAAGCCTGGCTGGCGGTTTTTCTTGGTTCTGCACTTTTCTGGGTGGTTGTCGCACTGCTGATTTGGAAAGTGTGGGGATAACTATGGTCGGTCAGGAGCAACTGGAGTCGTCACCATTATGCCAGCATAGTGACAATGaaa < 1:138272/251‑1 (MQ=255) cAATCGTTCAACGTTTTGCTGATAAGTGATGGCGATTATATGAGGTTAGCAATGCCTTCTGGAAATCAGGAACCCCGACGCGATCCTGAATTAAAACGTAAAGCCTGGCTGGCGGTTTTTCTTGGTTCTGCACTTTTCTGGGTGGTTGTCGCACTGCTGATTTGGAAAGCGTGGGGGTAACTATGGTCGGTCAGGCGCCACTGGAGTCGCCACCATTATGCCAGCATCGTGACAATGAAACAgaaacgaa > 1:533947/1‑250 (MQ=255) cAATCGTGCAACGTTTTGCTGATAAGTGATGGCGATTATATGATGTTAGCAATGCTTTCTGAAAATCAGGACCCGCGCCGCGATCCTTAATTAAAACGTAAAGCCTGGCTTGCGGTTTTTCTTGGTTCTGCACTTTTCTGGGTGGTTGTCGCACTGCTGATTTGGAAAGTGTGGGGATAACTATGTTCGGTCAGGAGCAACTGGAGTCGTCACCATTATGCCAGCATAGTGACAATGAAACAgaaacgaaa < 1:558250/251‑1 (MQ=255) aCGTTTTGCTGATAAGTGATGGCGATTATATGATGGTAGCAAGTCCTTCTGGAAATCAGGAACCCCGCCGCGATCCTGAATTAAAACGTAAAGCCTGGCTGGCGGTTTTTCTTGGGTCTGCACTTTTCTGGGTGGTTGTCGCACTGCTGATTTGGAAAGTGTGGGGATAACTATGGTCGGTCAGTAGCAACTGGAGTCGGCACCATTATGCCAGCATAGTGACAATGAAACAGAAACGAAACGGGAATGtt < 2:531088/251‑1 (MQ=255) | TTTGTTACTTATTTCTATACCGTGCACAATATTTTTATCCCTCATATTTATAGGGTAAATTACCTGTAAAGTAGTGTCTAATTACCCGCCTCGCAAACTCCAGTTTTCAAATATCTATTTCAGGTCAAAAGATTGAATTATTCACTGGAGACGATTCGTAAATTCGCTGG‑ATTTTTGACTAAAGTTTCTTTAGGATTTGTCTCATCGTATGATTAATTACACCAATCGTTCAACGTCTTGCTGATAAGTGATGGCGATTATATGAGGTTAGCAATGCCTTCTGGAAATCAGGAACCCCGCCGCGATCCTGAATTAAAACGTAAAGCCTGGCTGGCGGTTTTTCTTGGTTCTGCACTTTTCTGGGTGGTTGTCGCACTGCTGATTTGGAAAGTGTGGGGATAACTATGGTCGGTCAGGAGCAACTGGAGTCGTCACCATTATGCCAGCATAGTGACAATGAAACAGAAACGAAACGGGAATGTT > NC_000913/1334887‑1335369 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |