Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,322,759	G→A	A63V (GCT→GTT)	trpE ←	anthranilate synthase subunit TrpE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,322,759	0	G	A	100.0%	158.5 / NA	48	A63V (GCT→GTT)	trpE	anthranilate synthase subunit TrpE
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (20/28);  total (20/28)

ATGGCTTCTCGTTCTTCCTTCGGTACATTCAACAGATTCTGCAATAAACGGAAAGCGTCAAAAACCGAAAGGGAGCATAAGCGGGCGTCTTCATCCAGCAGTGGACTGACAGGGGGGAAGCGCAGCACACGGCAGTTTGGTGATTGTTCACTTTCCACACCCGCAGGCAGGGCGTTATCCAGTAGTGCCAGGAGGGCTTCGCCGTTGCCGGAAAGTGCCTGGATTGTGACAGTGTCACCTAAAGCTGTAATGCGCAGCGCACTGTCTACCAGCAGCAGGCTTTTTAAATCATCTTTGCTGTCGATATCTGCGGATTCCAGCAGCAGCGTTGCCGGACGATCCCCACACAACTGGTGAAAAAGCGCGGTGGGATTGTCGCGATAAGCGCCTTCGCAGGTTAGCAGTTCGAGAGTCGGTTTTTGTGTTTGCATTGTTATTCTCTAATTTTGTTCAAAAAAAAGCCCGCTCATTAGGCGGGCTGGGTATCTG  >  NC_000913/1322516‑1323004                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                      aTGGCTTCTCGTTCTTCCTTCGGTACATTCAACAGATTCTGCAATAAACGGAAAGCGTCAAAAACCGAAAGGGAGCATAAGCGGGCGTCTTCATCCAGCAGTGGACTGACAGGGGGGAAGCGCAGCACACGGCAGTTTGGTGATTGTTCACTTTCCACACCCGCAGGCAGGGCGTTATCCAGTAGTGCCAGGAGGGCTTCGCCGTTGCCGGAAAGTGCCTGGATTGTGACAGTGTCACCTAAAACTGTAAt                                                                                                                                                                                                                                                <  2:216472/251‑1 (MQ=255)    gCTTCTCGTTCTTCCTTCGGTACATTCAACAGATTCTGCAATAAACGGAAAGCGTCAAAAACCGAAAGGGAGCATAAGCGGGCGTCTTCATCCAGCAGTGGACTGACAGGGGGGAAGCGCAGCACACGGCAGTTTGGTGATTGTTCACTTTCCACACCCGCAGGCAGGGCGTTATCCAGTAGTGCCAGGAGGGCTTCGCCGTTGCCGGAAAGTGCCTGGATTGTGACAGTGTCACCTAAAACTGTAATgcg                                                                                                                                                                                                                                             <  1:299/251‑1 (MQ=255)       tctcGTTCTTCCTTCGGTACATTCAACAGATTCTGCAATAAACGGAAAGCGTCAAAAACCGAAAGGGAGCATAAGCGGGCGTCTTCATCCAGCAGTGGACTGACAGGGGGGAAGCGCAGCACACGGCAGTTTGGTGATTGTTCACTTTCCACACCCGCAGGCAGGGCGTTATCCAGTAGTGCCAGGAGGGCTTCGCCGTTGCCGGAAAGTGCCTGGATTGTGACAGTGTCACCTAAAACTGTAATgcgc                                                                                                                                                                                                                                            >  1:100990/1‑249 (MQ=255)                    tcGGTACATTCAACAGATTCTGCAATAAACGGAAAGCGTCAAAAACCGAAAGGGAGCATAAGCGGGCGTCTTCATCCAGCAGTGGACTGACAGGGGGGAAGCGCAGCACACGGCAGTTGGGTGATTGTTCACTTTCCACACCCGCAGGCAGGGCGTTATCCAGTAGTGCCAGGAGGGCTTCGCCGTTGCCGGAAAGTGCCTGGATTGTGACAGTGTCACCTAAAACTGGAATGCGCAGCGCACTGTCTAc                                                                                                                                                                                                                              <  2:50797/250‑1 (MQ=255)                     cGGTACATTCAACAGATTCTGCAATAAACGGAAAGCGTCAAAAACCGAAAGGGAGCATAAGCGGGCGTCTTCATCCAGCAGTGGACTGACAGGGGGGAAGCGCAGCACACGGCAGTTTGGTGATTGTTCACTTTCCACACCCGCAGGCAGGGCGTTATCCAGTAGTGCCAGGAGGGCTTCGCCGTTGCCGGAAAGTGCCTGGATTGTGACAGTGTCACCTAAAACTGTAATGCGCAGCGCACTGTCTAcc                                                                                                                                                                                                                             >  1:236838/1‑250 (MQ=255)                           aTTCAACAGATTCTGCAATAAACGGAAAGCGTCAAAAACCGAAAGGGAGCATAAGCGGGCGTCTTCATCCAGCAGTGGACTGACAGGGGGGAAGCGCAGCACACGGCAGTTTGGTGATTGTTCACTTTCCACACCCGCAGGCGGGGCGTTATCCAGTAGTGCCAGGAGGGCTTCGCCGTTGCCGGAAAGTGCCTGGATTGTGACAGTGTCACCTAAAACTGTAATGCGCAGCGCACTGTCTACcagcagca                                                                                                                                                                                                                      <  1:322252/251‑1 (MQ=255)                           aTTCAACAGATTCTGCAATAAACGGAAAGCGTCAAAAACCGAAAGGGAGCATAAGCGGGCGTCTTCATCCAGCAGTGGACTGACAGGGGGGAAGCGCAGCACACGGCAGTTTGGTGATTGTTCACTTTCCACACCCGCAGGCAGGGCGTTATCCAGTAGTGCCAGGAGGGCTTCGCCGTTGCCGGAAAGTGCCTGGATTGTGACAGTGTCACCTAAAACTGTAATGCGCAGCGCACTGTCTACcagcagc                                                                                                                                                                                                                       >  2:573139/1‑250 (MQ=255)                                        tGCAATAAAAGGCAAGCGTCAACAACCGCAAGGGAGCATAAGCGGGCGTCTTCATCCAGCAGTGGACTGACAGGGGGGAAGCGCAGCACACGGCAGTTTGGTGATTGTTCACTTTCCACACCCGCAGGCAGGGCGTTATCCAGTAGTGCCAGGAGGGCTTCGCCGTTGCCGGAAAGTGCCTGGATTGTGACAGCGTCACCTAAAACTGTAATGCGCAGCGCACTGTCTACCAGCAGCAGGCTTTTTAAat                                                                                                                                                                                                          >  2:366920/1‑250 (MQ=255)                                                 cGGAAAGCGTCAAAAACCGAAAGTGAGCATAAGCGGGCGTCTGCATCCAGCAGTGGACTGACAGGGGGGAAGCGCAGCACACGGCAGTTTGGGGATTGTTCACTTTCCACACCCGCAGGCAGGGCGTTATCCAGTAGGGCCAGGAGGGCGTCGCCGTTGCCGGAAAGTGCCTGGATTGTGACAGTGTCACCTAAAACTGTAATTCGCAGCGCACTGACTACCAGCAGCAGGATTTTTAAATCATCGTTGCt                                                                                                                                                                                                <  2:79024/251‑1 (MQ=255)                                                 cGGAAAGCGTCAAAAACCGAAAGGGAGCATAAGCGGGCGTCTTCATCCAGCAGTGGACTGACAGGGGGGAAGCGCAGCACACGGCAGTTTGGTGATTGTTCACTTTCCACACCCGCAGGCAGGGCGTTATCCAGTAGTGCCAGGAGGGCTTCGCCGTTGCCGGAAAGTGCCTGGATTGTGACAGTGTCACCTAAAACTGTAATGCGCAGCGCACTGTCt                                                                                                                                                                                                                                <  2:268802/219‑1 (MQ=255)                                                 cGGAAAGCGTCAAAAACCGAAAGGGAGCATAAGCGGGCGTCTTCATCCAGCAGTGGACTGACAGGGGGGAAGCGCAGCACACGGCAGTTTGGTGATTGTTCACTTTCCACACCCGCAGGCAGGGCGTTATCCAGTAGTGCCAGGAGGGCTTCGCCGTTGCCGGAAAGTGCCTGGATTGTGACAGTGTCACCTAAAACTGTAATGCGCAGCGCACTGTCt                                                                                                                                                                                                                                >  1:268802/1‑219 (MQ=255)                                                 cGGAAAGCGTCAAAAACCGAAAGGGAGCATAAGCGGGCGTCTTCATCCAGCAGTGGACTGACAGGGGGGAAGCGCAGCACACGGCAGTTTGGTGATTGTTCACTTTCCACACCCGCAGGCAGGGCGTTATCCAGTAGTGCCAGGAGGGCTTCGCCGTTGCCGGAAAGTGCCTGGATTGTGACAGTGTCACCTAAAACTGTAATGCGCAGCGCACTGTCTACCAGCAGCAGGCTTTTTAAATCATCTTTGCt                                                                                                                                                                                                >  1:280744/1‑251 (MQ=255)                                                     aaGCGTGAAAAACCGAAAGGGAGCATAAGCGGGCGTCTTCATCCAGCAGTGGAATGACAGGGGGGAAGCGCATCACACGGCAGTTTGGTGATTGTTCATGTTCCACACCCGCAGGCAGGGCGTTATCCAGTTGTGCCAGGAGGGCTTCGCCGTTGCCGGAAAGTGCCTTGATTGTGACAGTGTCACCTAAAACTGTAATGCGCAGCGCACTGTCTACCAGCAGCAGTCTTTTTAAATCATCTTTGCTGTCg                                                                                                                                                                                            <  2:431320/251‑1 (MQ=255)                                                     aaGCGTCAAAAACCGAAAGGGAGCATAAGCGGGCGTCTTCATCCAGCAGTGGACTGACAGGGGGGAAGCGCAGCACACGGCAGTTTGGTGATTGTTCACTTTCCACACCCGCAGGCAGGGCGTTATCCAGTAGTGCCAGGAGGGCTTCGCCGTTGCCGGAAAGTGCCTGGATTGTGACAGTGTCACCTAAAACTGTAATGCGCAGCGCACTGTCTACCAGCAGCAGGCTTTTTAAATCATCTTTGCTGTCg                                                                                                                                                                                            <  1:403425/251‑1 (MQ=255)                                                                  cGAAAGGGAGCATAAGCGGGCGTCTTCATCCAGCAGTGGACTGACAGGGGGGAAGCGCAGCACACGGCAGTTTGGTGATTGTTCACTTTCCACACCCGCAGGCAGGGCGTTATCCAGTAGTGCCAGGAGGGCTTCGCCGTTGCCGGAAAGTGCCTGGATTGTGACAGTGTCACCTAAAACTGTAATGCGCAGCGCACTGTCTACCAGCAGCAGGCTTTTTAAATCATCTTTGCTGTCGATATCTGCGGAtt                                                                                                                                                                               <  1:442275/251‑1 (MQ=255)                                                                     aaGGTAGCATAATCGGGCGTCTTCATCCAGCAGTGGACTGAGAGGGGGGAAGCGCAGCACACGGCAGTTTGGTGATTGTTCACTTTCCACACCCGCAGGCAGGGCGTTATCCAGTAGTGCCAGGAGGGCTTCGCCGTTGCCGGAAAGTGCCTGGATTGTGACAGTGTCACCTAAAACTGGAATGCGCAGCGCACTGTCTACCAGCAGCAGGCTTTTGAAATCATCTTTGCTGTCGATATCAGCGGATTCca                                                                                                                                                                            <  2:235717/251‑1 (MQ=255)                                                                     aaGGGAGCATAAGCGGGCGTCTTCATCCAGCAGTGGACTGACAGGGGGGAAGCGCAGCACACGGCAGTTTGGTGATTGTTCACTTTCCACACCCGCAGGCAGGGCGTTATCCAGTAGTGCCAGGAGGGCTTCGCCGTTGCCGGAAAGTGCCTGGATTGTGACAGTGTCACCTAAAACTGTAATGCGCAGCGCACTGTCt                                                                                                                                                                                                                                >  1:166365/1‑199 (MQ=255)                                                                     aaGGGAGCATAAGCGGGCGTCTTCATCCAGCAGTGGACTGACAGGGGGGAAGCGCAGCACACGGCAGTTTGGTGATTGTTCACTTTCCACACCCGCAGGCAGGGCGTTATCCAGTAGTGCCAGGAGGGCTTCGCCGTTGCCGGAAAGTGCCTGGATTGTGACAGTGTCACCTAAAACTGTAATGCGCAGCGCACTGTCt                                                                                                                                                                                                                                <  2:166365/199‑1 (MQ=255)                                                                               aaGCGGGAGTCTTCATCCAGCAGTGGACTGACAGGGGGGAAGCGCAGCACACGGCAGTTTGGTGATTGTTCACTTTCCACACCCGCAGGCAGGGCGTTATCCAGTAGTGCCAGGAGGGCTTCGCCGTTGCCGGAAAGTGCCTGGATTGTGACAGTGTCACCTAAAACTGTAATGCGCAGCGCACTGTCTACCAGCAGCAGGCTTTTTAAATCATCTTTGCTGTCGATATCTGCGGATTCCAGCAGCAGCGt                                                                                                                                                                  >  2:45650/1‑251 (MQ=255)                                                                                aGCGGGCGTCTTCATCCAGCAGTGGACTGACAGGGGGGAAGCGCAGCACACGGCAGTTTGGTGATTGTTCACTTTCCACACCCGCAGGCAGGGCGTTATCCAGTAGTGCCAGGAGGGCTTCGCCGTTGCCGGAAAGTGCCTGGATTGTGACAGTGTCACCTAAAACTGTAATGCGCAGCGCACTGTCTACCAGCAGCAGGCTTTTTAAATCATCTTTGCTGTCGATATCTGCGGATTCCAGCAGCAGCGtt                                                                                                                                                                 <  1:56376/251‑1 (MQ=255)                                                                                       gTCTTCATCCAGCAGTGGACTGACAGGGGGGAAGCGCAGCACACGGCAGTTTGGTGATTGTTCACTTTCCACACCCGCAGGCAGGGCGTTATCCAGTAGTGCCAGGAGGGCTTCGCCGTTGCCGGAAAGTGCCTGGATTGTGACAGTGTCACCTAAAACTGTAATGCGCAGCGCACTGTCTACCAGCAGCAGGCTTTTTAAATCATCTTTGCTGTCGATATCTGCGGATTCCAGCAGCAGCGTTGCCGGAc                                                                                                                                                          >  1:225835/1‑251 (MQ=255)                                                                                                 agcagTGGACTGACAGGGGGGAAGCGCAGCACACGGCAGTTTGGTGATTGTTCACTTTCCACACCCGCAGGCAGGGCGTTATCCAGTAGTGCCAGGAGGGCTTCGCCGTTGCCGGAAAGTGCCTGGATTGTGACAGTGTCACCTAAAACTGTAATGCGCAGCGCACTGTCTACCAGCAGCAGGCTTTTTAAATCATCTTTGCTGTCGATATCTGCGGATTCCAGCAGCAGCGTTGCCGGACGATCCCcac                                                                                                                                                 >  1:26884/1‑250 (MQ=255)                                                                                                    agTGGACTGCCAGGAGGGAAGCGCAGCACACGGACGTTTGGTGATTGTTCACTTTCCACACCCGCAGGCAGGGCGTTATCCAGTAGTGCCAGGAGGGCTTCGCCGTTGCCGGAAAGTGCCTGGATTGTGACAGTGTCACCTAAAACTGTAATGCGCAGCGCACTGTCTACCAGCAGCAGGCTTTTTAAATCATCTTTGCTGTCGATATCTGCGGATTCCAGCAGCAGCGTTGCCGGACGATCCCcacaca                                                                                                                                              >  1:181285/1‑250 (MQ=255)                                                                                                                    ggAAGCGCAGCACACGGCAGTTTGGTGATTGTTCACTTTCCACACCCGCAGGCAGGGCGTTATCCAGTATTGCCAGGAGGGCTTCGCCGTTGCCGGAAAGTGCCTGGATTGTGACAGTGTCACCTAAAACTGTAATGCGCAGCGCACTGTCTACCAGCAGCAGGCTTTTTAAATCATCTTTGCTGTCGATATCTGCGGATTCCAGCAGCAGCGTTGCCGGACGATCCCCACACAACTGGTGAAAAAGcgcg                                                                                                                             <  1:32025/251‑1 (MQ=255)                                                                                                                           cagcaCACGGCAGTTTGGTGATTGTTCACTTTCCACACCCGCAGGCAGGGCGTTATCCAGTAGTGCCAGGAGGGCTTCGCCGTTGCCGGAAAGTGCCTGGATTGTGACAGTGTCACCTAAAACTGTAATGCGCAGCGCACTGTCTACCAGCAGCAGGCTTTTTAAATCATCTTTGCTGTCGATATCTGCGGATTCCAGCAGCAGCGTTGCCGGACGATCCCCACACAACTGGTGAAAAAGCGCGGTGGGAt                                                                                                                      >  1:24289/1‑251 (MQ=255)                                                                                                                            agcaCACGGCAGTTTGGTGATTGTTCACTTTCCACACCCGCAGGCAGGGCGTTATCCAGTAGTGCCAGGAGGGCTTCGCCGTTGCCGGAAAGTGCCTGGATTGTGACAGTGTCACCTAAAACTGTAATGCGCAGCGCACTGTCTACCAGCAGCAGGCTTTTTAAATCATCTTTGCTGTCGATATCTGCGGATTCCAGCAGCAGCGTTGCCGGACGATCCCCACACAACTGGTGAAAAAGCGCGGTGGGAtt                                                                                                                     <  2:159590/251‑1 (MQ=255)                                                                                                                                    gCAGTTTGGTGATTGTTCACTTTCCACACCCGCAGGCAGGGCGTTATCCAGTAGTGCCAGGAGGGCTTCGCCGTTGCCGGAAAGTGCCTGGATTGTGACAGTGTCACCTAAAACTGTAATGCGCAGCGCACTGTCTACCAGCAGCAGGCTTTTTAAATCATCTTTGCTGTCGATATCTGCGGATTCCAGCAGCAGCGTTGCCGGACGATCCCCACACAACTGGTGAAAAAGCGCGGTGGGATTGTCGCGAt                                                                                                             <  1:139814/251‑1 (MQ=255)                                                                                                                                      cgcttGGTGATTGTTCACTTTCCACACCCGCAGGCAGGGCGTTATCCAGTAGTGCCCGGAGAGCTTCGCCGTTGCCGGAAAGTGCCTGGATTGTGACAGTGTCACCTAAAACTGTAATGCGCAGCGCACTGTCTACCAGCAGCAGGCTTTTTAAATCATCTTTGCTGTCGATATCTGCGGATTCCAGCAGCAGCGTTGCCGGACGCTCCCCACACAACTGGTGAAAAAGCGAGGTGGGATTGTCGCGaga                                                                                                            >  2:457081/4‑248 (MQ=255)                                                                                                                                      aGTTTGGTGATTGTTCACTTTCCACACCCGCAGGCAGGGCGTTATCCAGTAGTGCCAGGAGGGCTTCGCCGTTGCCGGAAAGTGCCTGGATTGTGACAGTGTCACCTAAAACTGTAATGCGCAGCGCACTGTCTACCAGCAGCAGGCTTTTTAAATCATCTTTGCTGTCGATATCTGCGGATTCCAGCAGCAGCGTTGCCGGACGATCCCCACACAACTGGTGAAAAAGCGCGGTGGGATTGTCGCGATa                                                                                                            >  1:482834/1‑250 (MQ=255)                                                                                                                                          tGGTGATTGTTCACTTTCCACACCCGCAGGCAGGGCGTTATCCAGTAGTGCCAGGAGGGCTTCGCCGTTGCCGGAAAGTGCCTGGATTGTGACAGTGTCACCTAAAACTGTAATGCGCAGCGCACTGTCTACCAGCAGCAGGCTTTTTAAATCATCTTTGCTGTCGATATCTGCGGATTCCAGCAGCAGCGTTGCCGGACGATCCCCACACAACTGGTGAAAAAGCGCGGTGGGATTGTCGCGATAAgcg                                                                                                        <  1:439182/250‑1 (MQ=255)                                                                                                                                               aTTGTTCACTTTCCACACCCGCAGGCAGGGCGTTATCCAGTAGTGCCAGGAGGGCTTCGCCGTTGCCGGAAAGTGCCTGGATTGTGACAGTGTCACCTAAAACTGTAATGCGCAGCGCACTGTCTACCAGCAGCAGGCTTTTTAAATCATCTTTGCTGTCGATATCTGCGGATTCCAGCAGCAGCGTTGCCGGACGATCCCCACACAACTGGTGAAAAAGCGCGGTGGGATTGTCGCGATAAGCGCCTTCg                                                                                                  <  1:178823/251‑1 (MQ=255)                                                                                                                                                  gTTCACTTTCCACACCCGCAGGCAGGGCGTTATCCAGTAGTGCCAGGAGGGCTTCGCCGTTGCCGGAAAGTGCCTGGATTGTGACAGTGTCACCTAAAACTGTAATGCGCAGCGCACTGTCTACCAGCAGCAGGCTTTTTAAATCATCTTTGCTGTCGATATCTGCGGAGTCCAGCAGCAGCGTTGCCGGACGATCCCCACACAACTGGTGAAAAAGCGCGGTGGGATTGTCGCGATAAGCGCCTTCGCa                                                                                                <  2:42197/250‑1 (MQ=255)                                                                                                                                                                     aggcaggGCGTTATCCAGTAGTGCCAGGAGGGCTTCGCCGTTGCCGGAAAGTGCCTGGATTGTGACAGTGTCACCTAAAACTGTAATGCGCAGCGCACTGTCTACCAGCAGCAGGCTTTTTAAATCATCTTTGCTGTCGATATCTGCGGATTCCAGCAGCAGCGTTGCCGGACGATCCCCACACAACTGGTGAAAAAGCGCGGTGGGATTGTCGCGATAAGCGCCTTCGCAGGTTAGCAGTTCGAGAGTCg                                                                            <  1:45650/251‑1 (MQ=255)                                                                                                                                                                        caggGCGTTATCCAGTAGTGCCAGGAGGGCTTCGCCGTTGCCGGAAAGTGCCTGGATTGTGACAGTGTCACCTAAAACTGTAATGCGCAGCGCACTGTCTACCAGCAGCAGGCTTTTTAAATCATCTTTGCTGTCGATATCTGCGGATTCCAGCAGCAGCGTTGCCGGACGATCCCCACACATCTGGTGAAAAAGCGCGGTGGGATTGTCGAGATAAGCGCCTTCGCAGGTTAGCAGTTCGAGAGTCGGtt                                                                         <  1:282471/251‑1 (MQ=255)                                                                                                                                                                                 aTCCAGTAGTGCCAGGAGGGCTTCGCCGTTGCCGGAAAGTGCCTGGATTGCGACAGTGTCACCTAAAACTGTAATGCGCAGCGCACTGTCTACCAGCAGCAGGCTTTTTAAATCATCTTTGCTGTCGATATCTGCGGATTCCAGCAGCAGCGTTGCCGGACGATCCCCACACAACTGGTGAAAAAGCGCGGTGGGATTGTCGCGATAAGCGCCTTCGCAGGTTAGCAGTTCGAGAGTCGGTTTTTGTGttt                                                                <  2:504699/251‑1 (MQ=255)                                                                                                                                                                                             cAGGAGGGCTTCGCCGTTGCCGGAAAGTGCCTGGATTGTGACAGTGTCACCTAAAACTGTAATGCGCAGCGCACTGTCTACCAGCAGCAGGCTTTTTAAATCATCTTTGCTGTCGATATCTGCGGATTCCAGCAGCAGCGTTGCCGGACGATCCCCACACAACTGGTGAAAAAGCGCGGTGGGATTGTCGCGATAAGCGCCTTCGCAGGTTAGCAGTTCGAGAGTCGGTTTTTGTGTTTGCATTGTTATtc                                                    >  1:242610/1‑251 (MQ=255)                                                                                                                                                                                                gaggGCTTCGCCGTTGCCGGAAAGTGCCTGGATTGTGACAGTGTCACCTAAAACTGTAATGCGCATCGCACTGTCTACCAGCAGCAGGCTTTTTAAATCATCTTTGCTGTCGATATCTGCTGATTCCAGCAGCAGCGTTGCCGGACGATCCCCACACAACTGGTGAAAAAGCGCGGTGGGATTGTCGCGATAAGCGCCTTCGCAGGTTAGCAGTTCGAGAGTCGGTTTTTGTGTTTGCATTGTTATtctcg                                                 <  2:190596/251‑2 (MQ=255)                                                                                                                                                                                                  gggCTTCGCCGTTGCCTGAAAGTGCCTGGATTGTGACAGTGTCACCTAAAACTGTAATGCTCAGCGCACTGTCTACCAGCAGCAGGCTTTTTAAATCATCTTTGCTGTCGATATCTGCGGATTCCAGCAGCAGCGTTGCCGGACGATCCCCACACAACTGGTGAAAAAGCGCGGTGGGATTGTCGCGATAAGCGCCTTCGCAGGTTAGCAGTGCGAGAGTCGGTGTTTGTGTTTGCATTGTTATTCTCTaa                                               <  2:76034/251‑1 (MQ=255)                                                                                                                                                                                                    gCTTCGCAGATGCCGCAACGTGCCTCGATTGTGACAGTATCCCCTCAAACTGTAAAGCGCAGCGCACCGTCTACCAGCAGCAGGCTTTTTAAATCATCTTTGCTGTCGATATCTGCGGATTCCAGCAGCAGCGTTGCCGGCCGCTCCCCACACCACTGGTGAACAAGCGCGGTGGGATTGTCGCGATAAGCGCCTTCGCAGGTGAGCAGATCGAGTGCCGGTTTTTGGGATTGCATTGTTATTCTCTAAtt                                             >  2:91732/1‑251 (MQ=255)                                                                                                                                                                                                       tCGCCGTTGCCGGAAAGTGCCTGGATTGTGACAGTGTCACCTAAAACTGTAATGCGCAGCGCACTGTCTACCAGCAGCAGGCTTTTTAAATCATCTTTGCTGTCGATATCTGCGGATTCCAGCAGCAGCGTTGCCGGACGATCCCCACACAACTGGTGAAAAAGCGCGGTGGGATTGTCGCGATAAGCGCCTTCGCAGGTTAGCAGTTCGAGAGTCGGTTTTTGTGTTTGCATTGTTATTCTCTAAttt                                            <  1:164500/249‑1 (MQ=255)                                                                                                                                                                                                           cGTTGCCGGAAAGTGCCTGGATTGTGACAGTGTCACCTAAAACTGTAATGCGCAGCGCACTGTCTACCAGCAGCAGGCTTTTTAAATCATCTTTGCTGTCGATATCTGCGGATTCCAGCAGCAGCGTTGCCGGACGATCCCCACACAACTGGTGAAAAAGCGCGGTGGGATTGTCGCGATAAGCGCCTTCGCAGGTTAGCAGTTCGAGAGTCGGTTTTTGTGTTTGCATTGTTATTCTCTAATTTTGTTCa                                      <  1:125252/251‑1 (MQ=255)                                                                                                                                                                                                           cGTTGCCGGAAAGTGACTGGATTGTGAAAGTGTCACCTCCAACTGTAATGCGCAGCGCACTGTCTACCAGAAGCAGGCTTTTTAAATCATCTTAGCTGTCGTTATCTGCGGATTCCAGCAGCAGCGTTGCCGGACGATCCCCACACAACTGGTGAAAACGCGCGGTGGGATTGTCGCGGTAAGCGCCTTCGCAGGTTAGCAGTTCGAGGGACGGTTTTTGTGTTTGCATTTTTATTCTCTAATTTTGttt                                       >  2:298558/1‑249 (MQ=255)                                                                                                                                                                                                              tGCCGGAAAGTGCCTGGATTGTGACAGTGTCACCTAAAACTGTAATGCGCAGCGCACTGTCTACCAGCAGCAGGCTTTTTAAATCATCTTTGCTGTCGATATCTGCGGATTCCAGCAGCAGCGTTGCCGGACGATCCCCACACAACTGGTGAAAAAGCGCGGTGGGATTGTCGCGATAAGCGCCTTCGCAGGTTAGCAGTTCGAGAGTCGGTTTTTGTGTTTGCATTGTTATTCTCTAATTTTGTTCaaa                                    <  2:482834/250‑1 (MQ=255)                                                                                                                                                                                                                      aGTGCCTGGATTGTGACAGGGTCACCTAAAACTGTAATGCGCAGCGCACTGGCTACCAGCATCAGGCTTTGTAAATCATCTTTGCTGTCGAGATCTGCGGATTCCAGCAGCAGCGTTGCCGGACGATCCCCTCTCAACTGGTGTAAAAGCTCGGTGGGATTGTCGCGATATTCTCCTTCGCATGTTAGCAGTTCGAGAGTCTGTTAGTGTGTTTGCATTGTTATTCGCTAATTTTGTTCAAAAAAAAGccc                           <  2:297801/251‑1 (MQ=255)                                                                                                                                                                                                                                    gACAGTGTCACCTAAAACTGTAATGCGCAGCGCACTGTCTACCAGCAGCAGGCTTTTTAAATCATCTTTGCTGTCGATATCTGCGGATTCCAGCAGCAGCGTTGCCGGACGATCCCCACACAACTGGTGAAAAAGCGCGGTGGGATTGTCGCGATAAGCGCCTTCGCAGGTTAGCAGTTCGAGAGTCGGTTTTTGTGTTTGCATTGTTATTCTCTAATTTTGTTCAAAAAAAAGCCCGCTCATTAGGCggg             <  2:203648/251‑1 (MQ=255)                                                                                                                                                                                                                                     aCAGTGTCACCTAAAACTGTAATGCGCAGCGCACTGTCTACCAGCAGCAGGCTTTTTAAATCATCTTTGCTGTCGATATCTGCGGATTCCAGCAGCAGCGTTGCCGGACGATCCCCACACAACTGGTGAAAAAGCGCGGTGGGATTGTCGCGATAAGCGCCTTCGCAGGTTAGCAGTTCGAGAGTCGGTTTTTGTGTTTGCATTGTTATTCTCTAATTTTGTTCAAAAAAAAGCCCGCTCATTAGGCGGGc            >  2:90373/1‑251 (MQ=255)                                                                                                                                                                                                                                          gtCACCTAAAACTGTAATGCGCAGCGCACTGTCTACCAGCAGCAGGCTTTTTAAATCATCTTTGCTGTCGATATCTGCGGATTCCAGCAGCAGCGTTGCCGGACGATCCCCACACAACTGGTGAAAAAGCGCGGTGGGATTGTCGCGATAAGCGCCTTCGCAGGTTAGCAGTTCGAGAGTCGGTTTTTGTGTTTGCCTTGTTATTCTCTAATTTTGTTCAAAAAAAAGCCCGCTCATTAGGCGGGCTGGGt       >  2:205343/1‑251 (MQ=255)                                                                                                                                                                                                                                               cTAAAACTGTAATGCGCAGCGCACTGTCTACCAGCAGCAGGCTTTTTAAATCATCTTTGCTGTCGATATCTGCGGATTCCAGCAGCAGCGTTGCCGGACGATCCCCACACAACTGGTGAAAAAGCGCGGTGGGATTGTCGCGATAAGCGCCTTCGCAGGTTAGCAGTTCGAGAGTCGGTTTTTGTGTTTGCATTGTTATTCTTTAATTTTGTTCAAAAAAAAGCCCGCTCATTAGGCGGGCTGGGTATCTg  >  1:221751/1‑251 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                      ATGGCTTCTCGTTCTTCCTTCGGTACATTCAACAGATTCTGCAATAAACGGAAAGCGTCAAAAACCGAAAGGGAGCATAAGCGGGCGTCTTCATCCAGCAGTGGACTGACAGGGGGGAAGCGCAGCACACGGCAGTTTGGTGATTGTTCACTTTCCACACCCGCAGGCAGGGCGTTATCCAGTAGTGCCAGGAGGGCTTCGCCGTTGCCGGAAAGTGCCTGGATTGTGACAGTGTCACCTAAAGCTGTAATGCGCAGCGCACTGTCTACCAGCAGCAGGCTTTTTAAATCATCTTTGCTGTCGATATCTGCGGATTCCAGCAGCAGCGTTGCCGGACGATCCCCACACAACTGGTGAAAAAGCGCGGTGGGATTGTCGCGATAAGCGCCTTCGCAGGTTAGCAGTTCGAGAGTCGGTTTTTGTGTTTGCATTGTTATTCTCTAATTTTGTTCAAAAAAAAGCCCGCTCATTAGGCGGGCTGGGTATCTG  >  NC_000913/1322516‑1323004

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑