Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,306,736	T→G	S325A (TCC→GCC)	oppF →	murein tripeptide ABC transporter/oligopeptide ABC transporter ATP binding subunit OppF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,306,736	0	T	G	100.0%	83.7 / NA	29	S325A (TCC→GCC)	oppF	murein tripeptide ABC transporter/oligopeptide ABC transporter ATP binding subunit OppF
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (11/18);  total (11/18)

CGGTAGAACTGGGGACCTATGATGAGGTCTACCACAATCCACTACATCCTTACACCAGGGCATTGATGTCGGCAGTCCCCATACCTGATCCGGATCTGGAGAAGAACAAAACCATCCAGTTACTGGAAGGGGAATTACCGTCGCCGATCAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACTCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAGAATGCGGGCAAAAACATCGTTGATTTCACCATCTTCACTGTCCGCCAGACCAATCACCACTTCGGCAAAAAAGTCCGGGTTCAAATCGAAATCAACATGTTCCTGCC  >  NC_000913/1306491‑1306981                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                      cGGTAGAACTGGGGACCTATGATGAGGTCTACCACAATCCACTACATCCTTACACCAGGGCATTGATGTCGGCAGTCCCCATACCTGATCCGGATCTGGAGAAGAACAAAACCATCCAGTTACTGGAAGGGGAATTACCGTCGCCGATCAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGtt                                                                                                                                                                                                                                                  >  2:288591/1‑251 (MQ=255)       aaCTGGGGACCTATGATGAGGTCTACCACAATCCACTACATCCTTACACCAGGGCATTGATGTCGGCAGTCCCCATACCTGATCCGGATCTGGAGAAGAACAAAACCATCCAGTTACTGGAAGGGGAATGACCGTCGCCGATCAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGc                                                                                                                                                                                                                                            <  2:277037/251‑1 (MQ=255)                    tgatgaGCTCTCCCACAATCCACTACCTCCTTACTCCAGGGCATTGATTGCGGCAGTCCCCATTCCTGAGCCGGATCTGCAGAAGAACAAACCCATCCATTGTCTGGAAGGGGAATTACCGCCGCCGATCAACCCGCCTTCCGTTTGTGTTTTCCGGACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACTTCCTGTTCTGGAGTGGAGTTTCTGACACGCCGTTTCTTGCCTGAAAGTCGAt                                                                                                                                                                                                                                <  1:351014/250‑1 (MQ=255)                     gatgaGGTCTACCACAATCCACTACATCCTTACACCAGGGCATTGATGTCGGCAGTCCCCATACCTGATCCGGATCTGGAGAAGAACAAAACCACCCAGTTACTGGAAGGGGAATTACCGTCGCCGATCAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATc                                                                                                                                                                                                                               <  1:288591/250‑1 (MQ=255)                        gaGGTCTACCACAATCCACTACATCCTTACACCAGGGCATGGATGTCGGCAGTCCCCATACCTGATCCGGATCTGGAGAAGAACAAAACCATCCAGTTACTGGAAGGGGAATTACCGTCGCCGATCAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCt                                                                                                                                                                                                                           <  2:124951/251‑1 (MQ=255)                                    aaTCCACTACTTCCTTACTCCAGGGCATTGATGTCGGCAGTCCCCATACCGGATCCGGATCTGGACAAGACCAAAACCATCCAGTTCCTGGAAGGGGAATTCCCGTCGCCGATCCTCCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACTCGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATa                                                                                                                                                                                                               <  1:28301/251‑1 (MQ=255)                                        cACTACATCCTTACACCAGGGCATTGATGTCGGCAGTCCCCATACCTGATCCGGATCTGGAGAAGAACAAAACCATCCAGTTACTGGAAGGGGAATTACCGTCGCCGATCAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAggg                                                                                                                                                                                                           >  2:264209/1‑251 (MQ=255)                                                        cAGGGCATTGATGTCGGCAGTCCCCATACCTGATCCGGATCTGGAGAAGAACAAAACCATCCAGTTACTGGAAGGGGAATTACCGTCGCCGATCAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGcc                                                                                                                                                                                           >  1:264825/1‑251 (MQ=255)                                                         ggggCAGTGATGTCGGCACGCCCCTTTCCGTATCCGTATCTGCAGAAGAACAAACCCATCCAGTTTCTGGAAGGGGAATTACCGTCGCCGATCAACCCGCCTTCCGGTTGTGTTTTCCTTCCCCGTTGCTTGATTGCCGGTCCACAGTGCGCCAAACCACTTCCTTTTCTGGAGGGGAGTTGCAGACACGCCGTGTCTTGCCTGAAGGTCGATTCACTTTAAAAACAATAATGGTTCAGAGTTTTCAGccc                                                                                                                                                                                          <  2:376605/250‑1 (MQ=255)                                                                  aTGTCGGCAGTCCCCATTCCTGGTCCGGTTCTGGAGAAAAACAAAACCATCCAGTTACTGGAAGGGGAATTTCCGTCGCCGATCAACCCCCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATGGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAATGGCTGACAGTTGTCAGCCCTTTTTCACg                                                                                                                                                                                 <  2:553595/251‑1 (MQ=255)                                                                            gccccATACCTGATCCGGATCTGGAGAAGAACAAAACCATCCAGTTACTGGAAGGGGAATTACCGTCGCCGATCAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGa                                                                                                                                                                       <  1:553478/250‑1 (MQ=255)                                                                              cccATACCTGATCCGGATCTGGAGAAGAACAAAACCATCCAGTTACTGGAAGGGGAATTACCGTCGCCGATCAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGACTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGatt                                                                                                                                                                     >  2:436228/1‑251 (MQ=255)                                                                                                  ggaaaagaaCAAAACCATCCATTTGCTGGAAGGGGAATTCCCGTCGCCGATCCACCCGCCTGCCGGTTGTTTTTTCCTTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTTAAATTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAgatgat                                                                                                                                                 <  1:341647/247‑1 (MQ=255)                                                                                                                        ttACTGGAAGGGGAATTACCGTCGCCGATCAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAACCAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGACAAAGTTTGTGATCtttt                                                                                                                           >  2:344371/1‑251 (MQ=255)                                                                                                                            gggAAGGGGAATTACCGTCCACGATCAACCCTCCTTCCGGTTGTGTGTTCCGTACCAGTTGGCCGATGGCCGGTCCAGGGGTCGCAAAAACACGTCCTGTTCTGGAGGGGTGTTCCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTGTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATTTGGCAAAGTTTTTGGGCTTTTTcgc                                                                                                                       <  2:399611/250‑1 (MQ=255)                                                                                                                             ggAAGGGGAATTACCGTCGCCGATCAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGCGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTcgcg                                                                                                                      >  1:207525/1‑251 (MQ=255)                                                                                                                                 ggggaacgcccctcaccGATGAAACCGCCTTACGCTTGTGTTTTCCGTCCCCGTTACCAGCTTGCCGGCCCAGAGTGCGACAAAACACGTCCTGTTCTGGCGGGGAGTTTCCGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATACGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTCTTCACGCCAGATGATATGGCACAGTTTGTGATCTTTTTCGCGGCAt                                                                                                                  >  2:67482/16‑251 (MQ=255)                                                                                                                                        tACCGTCGCCGATCAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAGa                                                                                                            >  1:151881/1‑250 (MQ=255)                                                                                                                                                   aTCAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAGAATGCGGGCaaa                                                                                                 >  1:203595/1‑250 (MQ=255)                                                                                                                                                        cccGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAGAATGCGAGCAAAAACATc                                                                                           >  1:494593/1‑251 (MQ=255)                                                                                                                                                               tCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAGAATGCGGGCAAAAACATCGTTGAtt                                                                                    >  1:291954/1‑251 (MQ=255)                                                                                                                                                                ccGGGTGTGTTTGCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCTCTTTAAAAACAATAAGGGCGGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAGAATGCGGGCAAAAACATCGTTGAttt                                                                                   <  1:62583/251‑1 (MQ=255)                                                                                                                                                                  ggTTGTGTTTTCCGTACCCGTTGCCCGATTGGCGGTCCCGAGTGCGCCAAAACACGTCCTTTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGTCATAACAGAATGCGGGCAAAAACATCGTTGATTTCa                                                                                 <  2:503875/251‑1 (MQ=255)                                                                                                                                                                      gtgtTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAGAATGCGGGCAAAAACATCGTTGATTTCACCAt                                                                             <  2:207525/251‑1 (MQ=255)                                                                                                                                                                           ttCCGTACCCGTTGCCCGATTTCCGGTCCAGAGTGCCCCAAATCACGTCCTGTGCGGGAGGTGAGTTTCAGACACGCCTTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGATGGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAGAATGCGGGCAAAAACATCGTTGATTTCACCATCTTc                                                                         <  1:300694/250‑1 (MQ=255)                                                                                                                                                                                                                  aaaaCACGTCCTGGTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCATATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACATAATGCGGGCAAAAACATCGGTGATTTCACCATCTTCGCTGTCCGCCAGACCAATCACCACGTCGGCAAAAAAGTcc                                 <  2:291954/251‑1 (MQ=255)                                                                                                                                                                                                                    aaCACGTACTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCGGACAGTTGTCAGCACTTTTGCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCAGAACAGAATGCGGGCAAAAACATCGTTGATTTCACCATCTTCACTGTCCGCCAGACCAATCACCACTTCGGCAAAAAAGTCCgg                               <  1:256725/251‑1 (MQ=255)                                                                                                                                                                                                                                   ggAGGGGATTTTCAGACACGCCGTTTCTTGCCTGAAAGTCTATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCATCCCTTTTTCACGCTAAAAGCGATTATTGATTCCCGCCAGATGATATGGCAAAGTTTGTGAGCTTTTGCGCGGCATAACAGAATGCGGGCAAAAACATCGTAGATTTCACCATCTTCACTTTCCGCCAGACCAATTACCACTTCTGCAAAAAAGTTGGGGTTCAAATCGAAAt                 <  2:568661/250‑1 (MQ=255)                                                                                                                                                                                                                                                 aacacGCCGTTTCTTGCCTGAAAGTCGATCCTCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATGCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAGAATGCGGTCAAAAACATCGTTGATTTCACCATCTTCACTGTCCTCCAGACCAATCACCACTTCGGCAAAAAAGTCCGGGTCCAAATCGAAATCAACATGTTCCTGcc  <  1:369305/250‑1 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                      CGGTAGAACTGGGGACCTATGATGAGGTCTACCACAATCCACTACATCCTTACACCAGGGCATTGATGTCGGCAGTCCCCATACCTGATCCGGATCTGGAGAAGAACAAAACCATCCAGTTACTGGAAGGGGAATTACCGTCGCCGATCAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACTCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAGAATGCGGGCAAAAACATCGTTGATTTCACCATCTTCACTGTCCGCCAGACCAATCACCACTTCGGCAAAAAAGTCCGGGTTCAAATCGAAATCAACATGTTCCTGCC  >  NC_000913/1306491‑1306981

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑