| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,269,702 | C→T | intergenic (+313/+1) | rdlA → / ← ldrB | putative antisense regulatory RNA RdlA/small toxic polypeptide LdrB |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,269,702 | 0 | C | T | 90.9% | 75.2 / ‑1.9 | 33 | intergenic (+313/+1) | rdlA/ldrB | putative antisense regulatory RNA RdlA/small toxic polypeptide LdrB |
| Reads supporting (aligned to +/- strand): ref base C (1/2); new base T (17/13); total (18/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.79e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TTGGTGTGGATACTACGACTCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTGCATTCCGGTTTTTCAACAGCTGTTACAGTCATTTCATGAGTGCTCTGGATGAGGCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGTGATAAAGACAAAAATGGCCGCAGGCTGTTACCCCTGCGGCCGGTTTCGGGCGCATATTGCCATCACGGCAGCCTGACGCCCGTTTTCACCTTACTTCCGGTTACGCCACCAGCCGACAATCGCTGCGGTAATAATTCCCGCCAGGATCGGTGCCGCCAGGTCGTGCCAGAAAGTCAT‑GGCAAACTGCGCGAGCGTCATATAGCCGCCTTGTTGTAATGACAACATTTTGCGGCTATTCTTGA‑AGTGTCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCTCTTTCCAGCA > NC_000913/1269457‑1269933 | ttGGTGTGGATACTACGACTCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTGCATTCCGGTTTTTCAACAGCTGTTACAGTCATTTCATGAGTGCTCTGGATGAGGCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGTGATAAAGACAAAACTGGCCGCAGGCTGTTACCCCTGCGGCCGGTTTCGGGCGCATATTGCCATCACGGCAGCCTGACGCCCGTTTTCACTTTACt > 2:518555/1‑251 (MQ=255) gggTGGGGATACGACGACTCAATTCATCTTCACTTCATCCCGGAAATGATTGCAATGAAGAGTGCATTCCGGTGTTTCAACAGCTGTGACAGGCATTTCATGAGTGCTCGAGAGGAGGATTCCAGCTCGGGTTGCGAATATTTACTTGTGGAAGTGATAAAGACAAAAATGGCCGCAGGCTGTTACCCCTGCGGCCGGTTTCGGGCGCATATTGCCATCACGGCAGCCTGACGCCCGTTTTCACTTTACtt < 1:88986/250‑1 (MQ=14) ctactaCTCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATAAAGAGTGCATTCCGGTTTTTCAACATCTGTTGCAGTCATTGCATGAGTGCTCTGGATGAGTCTTCCAGCTCGGGTTTCCAATATTTACTTGTGGAAGTGATAAAGACAAAAATGGCCGCAGGCTGTTACCCCGGCGGCCGGTTTCGGGCGCATATTGCCATCACGGCAGCCTGACGCCCGTTTTCACCTTACtt < 2:586097/240‑1 (MQ=11) ctacGACTCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTGCATTCCGGTTTTTCAACAGCTGTTACAGTCATTTCATGAGTGCTCTGGATGAGGCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGAGATAAAGACAAAAATGGCCGCAGGCTGTTACCCCTGCGGCCGGTTTCGGGCGCATATTGCCATCACGGCAGCCTGACGCCCGTTTTCACTTTACTTCGGGTTACGcc > 1:586097/1‑251 (MQ=255) aTTCATCTTCACTTCATCCATGAACTGTTTGCAATGCAGGTTGCATTTCGGTTTTTCAACAGCTGTTACAGTCATTTCATGCGTGCTCTGGATGAGGCTTCCAGCTCGGGTTGCCATTATTTACTTGTGGAAGTGATAAAGACACAAATGGACACAGGCTGTTACCCATGCGGCCGGCTTCGGCCGCATATTGCCATCACGACAACCTGCCGCCCGTTTTCACTTTACTTCCGGTTACGCCACCAGCCGAc > 2:44481/1‑251 (MQ=255) cttcacttcaTCCCTGAAATGTTTGCAATGAAGAGTGCATTCCGGTTTTTCAACAGCTGTTACAGTCATTTCATGAGTGCTCTGGATGAGGCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGTGATAAAGACAAAAATGGCCGCAGGCTGTTACCCCTGCGGCCGGTTTCGGGCGCATATTGCCATCACGGCAGCCTGACGCCCGTTTTCACTTTACTTCCGGTTACGCCACCAGCCGACAATCGc > 1:219468/1‑251 (MQ=255) tcacttcaTCCCTGAAATGTTTGCAATGAATAGTGCATTCCGGTTTTTCAACAGCTGTTACAGTCATTTCATGAGTGCTCTGGATGAGGCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGTGATAAAGACAAAAATGGCCGCAGGCTGTTACCCCTGCGGCCGGTTTCGGGCGCATATTGCCATCACGGCAGCCTGACGCCCGTTTTCACTTTACTTCCGGTTACGCCACCAGCCGACAATc < 1:6081/247‑1 (MQ=255) tcacttcaTACCTGCAATGTTTGCAATGAAGAGTGCATTCCGGTTTTTCAACAGCTGTTACAGTCCTTTCATGAGTGCTCTGGATGAGGCTTCCAGCTCGGGCTGCCCATATTTACGTGTGGAAGTGATAAAGACAAAAATGGCCGCAGGCTGTTACCCCTGCGGCCGGTTTCGGGCGCATATTGCCATCCAGGCATCCTCACGCCCGCTTTCACTTTACTTCCGGTTTCGCCACCAGCCGACAATCGcgg > 2:543579/1‑249 (MQ=14) aaaTGTTTGCAATGAAGAGTGCATTCCGGTTTTTCAACAGCTGTTACAGTCATTTCATGAGTGCTCTGGATGATGCTTCCAGCTCGGGTTGCCAATATTATCTGGTGGGCGTCGTGTAGGCAAAGAGGGTAGCTCTCTGTTGCCCCTGTGTCCGTTTAAGGACGCATATTGCCATCACGGCAGCCTGACGCCCGTGTTCCCTTTACTTCCTGTTACGCCACCAGCCGAAAATCTAGGCAGTAATAATTccc > 2:148286/1‑251 (MQ=9) aaTGAAGAGTGCATTCCGGTTTTTCAACAGCTGTTACAGTCATTTCATGAGTGCTCTGGATGAGGCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGTGATAAAGACAAAAATGGCCGCAGGCTGTTACCCCTGCGGCCGGTTTCGGGCGCATATTGCCATCACGGCAGCCTGACGCCCGTTTTCACTTTACTTCCGGTTACGCCACCAGCCGACAATCGCTGCGGTAATAATTCCCGCCAGGATCg > 1:539105/1‑251 (MQ=255) aaTGAAGAGTGCATTCCGGTTTTTCAACAGCTGTTACAGTCATTTCATGAGTGCTCTGGATGAGGCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGTGATAAAGACAAAAATGGCCGCAGGCTGTTACCCCTGCGGCCGGTTTCGGGCGCATATTGCCATCACGGCAGCCTGACGCCCGTTTTCACTTTACTTCCGGTTACGCCACCAGCCGACAATCGCTGCGGTAATAATTCCCGCCAGGATCg > 2:435165/1‑251 (MQ=255) tACAGTCATTTCATGAGTGCTCTGGATGAGGCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGTGATAAAGACAAAAATGGCCGCAGGCTGTTACCCCTGCGGCCGGTTTCGGGCGCATATTGCCATCACGGCAGCCTGACGCCCGTTTTCACTTTACTTCCGGTTACGCCACCAGCCGACAATAGCTGCGGTAATAATTCCCGCCAGAATCGGTGCCGCCAGGTCGTGCCTGAAAGTCAT‑GGCaa > 1:509738/1‑250 (MQ=255) cATGAGTGCTCTGGATGAGGCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGTGATAAAGACAAAAATGGCCGCAGGCTGTTACCCCTGCGGCCGGTTTCGGGCGCCTCTTGCCATCACGGCAGCCTGACGCCCGTTTTCACTTTACTTCCGGTTACGCCACCAGCCGACAATCGCTGCGGTAATAATTCCCGCCAGGATCGGTGCCGCCAGGTCGTGCCAGAAAGTCAT‑GGCAAACTGCGCGAGCg > 1:547834/1‑251 (MQ=255) tgcggcctccagaccGGGTTGCCAATCTTTACTTGTGCAAGTGATAAAGACAAAAATGGCCGCAGGATGTTACCCCTGCGGCCGGTTTCGGGCGCATATTGCCATCACGGCAGCCTGACGCCCGTTTTCACTTTACTTACGGTTACGCCACCAGCCGACAATCGCTCCGGTAATAATTCCCGCCAGGATAGGTGCCGCCAGGTCGTGCCAGAAAGTCCT‑GGCAAACTGCGAGAGCGCAATATAGCCGCCtt > 2:563742/15‑251 (MQ=11) gCCAATATTTACTTGTGGAAGTGATAAAGACAAAAATGGCCGCAGGCTGTTACCCCTGCGGCCGGTTTCGGGCGCATATTGCCATCACGGCAGCCTGACGCCCGTTTTCACTTTACTTCCGGTTACGCCACCAGCCGACAATCGCTGCGGTAATAATTCCCGCCAGGATCGGTGCCGCCAGGTCGTGCCAGAAAGTCAT‑GGCAAACTGCGCGAGCGTCATATAGCCGCCTTGTTGTAATGACAACAtttt < 1:144066/250‑1 (MQ=37) ccAATATTTACTGGTGGAAGTGATAAAGACAAAAAGGGCCGCAGGCTGTTACCCCTGCGGCAGGTTTCGGGCGCATATTGCCATCACGGCAGCCTGACGCCCGTTTTCACTTTACTTCCGGTTACGCCACCAGCCGACAATCGCTGCGGTAATATTTCCCGCCAGGAGCGGTGCCGCCAGGTCGTGCCAGAAAGTCAT‑GGCAAACTGCGCGAGCGTCATATAGCCGCCTTGTTGTAATGACAACATTTTg < 1:29114/250‑1 (MQ=255) cAATATTTACTTGTGGAAGTGATAAAGACAAAAATGGCCGCAGGCTGTTACCCCTGCGGCCGGTTTCGGGCGCATATTGCCATCACGGCAGCCTGACGCCCGTTTTCACTTTACTTCCGGTTACGCCACCAGCCGACATTCGCGGCGGTAATAATTCCCGCCAGGAGCGGTGCCGCCAGGTCGTGCCAGAAAGTCAT‑GGCAAACTGCGCGAGCGTCATATAGCCGCCTTGTTGTAATGACAACATTTTGCg < 1:549218/251‑1 (MQ=255) cAATATTTACTTGTGGAAGTGATAAAGACAAAAAGGGCCGCAGGCTGTTACCCCTGCGGCCGGTTTCGGGCGCATATTGCCATCACGGCAGCCTGACGCCCGTTTTCACTTTACTTCCGGTTACGCCACCAGCCGACAATCGCTGCGGTAATAATTCCCGCCAGGATCGGTGCCGCCAGGTCGTGCCAGAAAGTCAT‑GGCAAACTGCGCGAGCGTCATATAGCCGCCTTGTTGTAATGACAACATTTTGCg < 1:435165/251‑1 (MQ=37) tatTTACTTGTGGAAGTGATAAAGACAAAAATGGCCGCAGGCTGTTACCCCTGCGGCCGGTTTCGGGCGCATATTGCCATCACGGCAGCCTGACGCCCGTTTTCACTTTACTTCCGGTTACGCCACCAGCCGACAATCGCTGCGGTAATAATTCCCGCCAGGATCGGTGCCGCCAGGTCGTGCCAGAAAATCAT‑GGCAAACTGCGCGAGCGTCATATAGCCGCCTTGTTGTAATGACAACATTTTGCGGCt > 2:82842/1‑251 (MQ=18) ttcttggaggtgtgctAAATACAAAAATGGCCGCAGGCTCTTACCCCTGCGGCCGGTTTCGGGCGCATATTTCCATCACGGCAGCCGGACGCCCGTTTCCCCTTTACTTCGGGTTACGCCACCAGCTGCCAAGCTCTGGGGTAAAAATTCCCGCCAGGATCGGTGGCGCCAGGTCGTGCCAGAAAGTCAT‑GGCAAACGGCGCGAGCGTCATATAGCCGCCTTGTTGTAATGACAACATTTTGCGGCTAtt < 1:580129/235‑1 (MQ=11) gATAACGACAAAAATGGCCGCAGGCTGTTACCCCTGCGGCCGGTTTAGGGCGCATATTGTCCTCCCGGCAGCCTGACGCCCGTTTTCCCTTTACTTCCGGTTCCGCCACCAGCCGACaa > 2:541098/1‑119 (MQ=11) gATAAAGACAAAAATGGCCGCAGGCTGTTACCCCTGCGGCCGGTTTCGGGCGCATATTGTCATCACGGCAGCCTGACGCCCGTTTTCACTTTACTTCCGGTTACGCCACCAGCCGACaa < 1:541098/119‑1 (MQ=255) ccGCAGGCTGTTACCCCTGCGGCCGGTTTCGGGCGCATATGGCCATCACGGCAGCCTGCCGCCCTTTTTCACTTTACTTCCGGTTACGCCACCAGCCGACAATCGCTGCGGTAATAATTCCCGCCAGGATCGGTGCCGCCAGGTCGTGCCAGAAAGTCAT‑GGCAAACTGCGCGAGCGTCATATAGCCGCCTTGTTGTAATGACAACATTTTGCGGCTATTCTTGA‑AGTGTCTGGTTTCAAGATTAGccccc < 1:251265/251‑1 (MQ=255) ggCTGTTCCCCCTGCGGCCGGTTTCGGGCGCATATGGCCATCACGGCAGCCTGACGCCCGTTTTCACTTTACTTCCGGTTACGCCACCAGCCGACAATCGCGGCGGTAATAATTCCCGCCAGGATCGGTGCCGCCAGGTCGTGCCAGAAAGTCAT‑GGCAAACTGCGCGAGCGTCATATAGCCGCCTTGTTGTAATGACAACATTTTGCGGCTATTCTTGA‑AGTGTCTGGTTTCAAGATTAGCCCCCGTTCt < 2:26417/251‑1 (MQ=255) tGTTACCCCTGCGGCCGGTTTCGGGCGCATATTGCCATCACGGCAGCCTGACGCCCGTTTTCACTTTACTTCCGGTTACGCCACCAGCCGACAATCGCTGCGGTAATAATTCCCGCCAGGATCGGTGCCGCCAGGTCGTGCCAGAAAGTCAT‑GGCAAACTGCGCGAGCGTCATATAGCCGCCTTGTTGTAATGACAACATTTTGCGGCTATTCTTGA‑AGTGTCTGGTTTCAAGATTAGCCCCCGTTCtgtt > 1:576064/1‑251 (MQ=37) cccTGAGGCAGGTTTCGGGCGCATATTGCCATCAAGGCAGCCTGCCGCCCGTTTTCACTTTACTTCCGGTTACGCCACCAGACGACAATCGCTGCGGAAATAATTCCCGCCAGGATCGGTGCCGCCAGGTCGTGCCAGAAAGTCAT‑GGCAAACTGCGCGAGCGTCATATAGCCGCCTTGTTGTAATGACAACATTTTGCGGCTATTCTTGA‑AGTGTCTGGTTTCACGATTAGCCCCCGTTCTGTTGTCAgg > 2:562420/1‑251 (MQ=11) gCGGCCGGTTTCGGGCGCATATTGCCATCACGGCAGCCTGACGCCCGTTTTCACTTTACTTCCGGTTACGCCACCAGCCGACAATCGCTGCGGTAATAATTCCCGCCAGGATCGGTGCCGCCAGGTCGTGCCAGAAAGTCAT‑GGCAAACTGCGCGAGCGTCATATAGCCGCCTTGTTGTAATGACAACATTTTGCGGCTATTCTTGA‑AGTGTCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGttt < 2:204721/250‑1 (MQ=37) cGGGCGCATATTGCCATCCCGGCAGCCTGACGCCCGTTTTCACTTTACTTCCGGTTACGCCACCAGCCGACAATCGCTGCGGTAATAATTCCCGCCAGGATCGGTGCCGCCAGGTCGTGCCAGAAAGTCAT‑GGCAAACTGCGCGAGCGTCATATAGCCGCCTTGTTGTAATGACAACATTTTGCGGCTATTCTTGA‑AGTGTCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAAc < 1:518555/250‑1 (MQ=37) gggCGCATGTGGCCATCACGGCAGCCTGACGCCCTTTTCCACCTTACTTCCGGTTACGCCACCAGCCGAAAATCGCTGCGGTAATAATTCCCGCCAGGATCGGTGCTGCCAGGTCGTGCCAGAAAGTCAT‑GGCAAACTGCGCGAGCGTCATATAGCCGCCTTGTTGTAATGACAACATTTTGCGGCTATTCTTGA‑AGTGTCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGt < 2:432041/251‑1 (MQ=255) cgcatatgcccagcccGGCAGCCTGACGCCGGTTTTCTCTTTACTTCCGGTTACGCCCCCAGCGGACAATCTCTGCGGTAATAATCCCCGCCAGGATCGGTGCCGCCAGGTCGTGCCAGAAAGTCAT‑GGCAAACTGCGCGAGCGTCATACAGCCGCCTTGTTGTAATTACAACATTGTGCGGCTATTCTTGA‑AGTGTCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCg < 1:393860/236‑1 (MQ=11) cATATTGCCATCACGGCAGCCTGACGCCCGTTTTCACTTTACTTCCGGTTACGCCACCAGCCGACAATCGCTGCGGTACTAATTCCCGCCAGGATCGGTGCCGCCAGGTCGTGCCAGAAAGTCAT‑GGCAAACTGCGCGAGCGTCATATAGCCGCCTTGTTGTAATGACAACATTTTGCGGCTATTCTTGA‑AGTGTCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCggg > 2:266846/1‑251 (MQ=37) atTGCCATCACGGCAGCCTGACGCCCGTTTTCACCTTACTTCCGGTTACGCCACCAGCCGACAATCGCTGCGGTAATAATGGCCGCGCGTCGCGTAGGCACCGGGT‑GTAGCTCTCGGTGGTCGCCGAATTGTTCGAGAGACAAATAGCAGCCTTGTTGTCATGACAACATTTTGTTGCTATTCTTGACAG‑GTCTGGTTTCAAGACTAGCCCCATTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGt > 2:67891/1‑251 (MQ=2) ccTGACGCCCGTTTTCACTTTACTTCCGGTTACGCCACCAGCCGACAATCGCCGCGGTAATAATTACCGCCAGGATCGGTGCCGCCAGGTCGTGCCAGAAAGTCAT‑GGCAAACTGCGCGAGCGTCATATAGCCGCCTTGTTGTAATGACAACATTTTGCGGCTATTCTTGA‑AGTGTCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCTCTTTCCAGCa > 1:561571/1‑250 (MQ=255) | TTGGTGTGGATACTACGACTCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTGCATTCCGGTTTTTCAACAGCTGTTACAGTCATTTCATGAGTGCTCTGGATGAGGCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGTGATAAAGACAAAAATGGCCGCAGGCTGTTACCCCTGCGGCCGGTTTCGGGCGCATATTGCCATCACGGCAGCCTGACGCCCGTTTTCACCTTACTTCCGGTTACGCCACCAGCCGACAATCGCTGCGGTAATAATTCCCGCCAGGATCGGTGCCGCCAGGTCGTGCCAGAAAGTCAT‑GGCAAACTGCGCGAGCGTCATATAGCCGCCTTGTTGTAATGACAACATTTTGCGGCTATTCTTGA‑AGTGTCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCTCTTTCCAGCA > NC_000913/1269457‑1269933 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |