Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	31,683	C→T	N289N (AAC→AAT)	carB →	carbamoyl‑phosphate synthetase large subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	31,683	0	C	T	100.0%	177.7 / NA	59	N289N (AAC→AAT)	carB	carbamoyl‑phosphate synthetase large subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (24/35);  total (24/35)

GAGTCGCTGATCGGCTGGAAAGAGTACGAGATGGAAGTGGTGCGTGATAAAAACGACAACTGCATCATCGTCTGCTCTATCGAAAACTTCGATGCGATGGGCATCCACACCGGTGACTCCATCACTGTCGCGCCAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGG‑TGGGTTACACCCTCGACGAACTGATGAACGACATCACTGGCGGACGTACTCCGGCCTCCTTCGAGCCGTCCATCGACTATGTGGTTACTAAAATTCCTCGCTTCAACTTCGAAAAA  >  NC_000913/31438‑31914                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                         gaGTCGCTGATCGGCTGGAAAGAGTACGAGATGGAAGTGGTGCGTGATAAAAACGACAACTGCATCATCGTCTGCTCTACCGAAAACTTCGATGCGATGGGCATCCCCACCGGTGACTCCATCACTGTCGCGCCAGCCCAAACGCTGACCGACAAAGAATAACAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGaa                                                                                                                                                                                                                                     <  1:568667/251‑1 (MQ=255)       cTGATCGGCTGGAAAGAGTACGAGATGGAAGTGGTGCGTGATAAAAACGACAACTGCATCATCGTCTGCTCTATCGAAAACTTCGATGCGATGGGCATCCACACCGGTGACTCCATCACTGTCGCGCCAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGGGAATCCGAAAAACgg                                                                                                                                                                                                                               >  2:129944/1‑251 (MQ=255)                 ggAAGGAGTACGAGATGGAAGTGGTGCGTGATAAAAACGACAACTGCATCATCGTCTGCTCTATCGAAAACTTCGATGCGATGGGCATCCACACCGGTGACTCCATCACTGTCGCGCCAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGTTCGTCTGAtt                                                                                                                                                                                                                     <  1:149463/251‑1 (MQ=255)                 ggAAAGAGTACGAGATGGAAGTGGTGCGTGATAAAAACGACAACTGCATCATCGTCTGCTCTATCGAAAACTGCGATGCGATGGGCATCCACACCGGTGACTCCATCACTGTCGCGCCAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGGTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGa                                                                                                                                                                                                                       <  2:327671/249‑1 (MQ=255)                     agagTACGAGATGGAAGTGGTGCGTGATAAAAACGACAACTGCATCATCGTCTGCTCTATCGAAAACTTCGATGCGATGGGCATCCACACCGGTGACTCCATCACTGTCGCGCCAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTa                                                                                                                                                                                                                 <  2:34268/251‑1 (MQ=255)                      gagTACGAGATGGAAGTGGTGCGTGATAAAAACGACAACTGCATCATCGTCTGCTCTATCGAAAACTTCGATGCGATGGGCATCCACACCGGTGACTCCATCACTGTCGCGCCAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTAt                                                                                                                                                                                                                <  2:164375/251‑1 (MQ=255)                      gagTACGAGATGGAAGTGGTGCGTGATAAAAACGACAACTGCATCATCGTCTGCTCTATCGAAAACTTCGATGCGATGGGCATCCACACCGGTGACTCCATCACTGTCGCGCCAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTAt                                                                                                                                                                                                                <  1:326570/251‑1 (MQ=255)                               gtGGAAGTGGTGCGTGATAAAAACGACAACTGCATCATCGTCTTCTCTATCGAAAACTTCGATGCGATGGTCATCCACACCGGTGACTCCATCACTGTCGCGCCAGCCCAAACTCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATTGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGTTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGaa                                                                                                                                                                                                       <  1:48989/250‑1 (MQ=255)                               aTGGAAGTGGTGCGTGATAAAAACGACAACTGCATCATCGTCTGCTCTATCGAAAACTTCGATGCGATGGGCATCCACACCGGTGACTCCATCACTGTCGCGCCAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTTTGATCGAAATGaa                                                                                                                                                                                                       <  2:342385/251‑1 (MQ=255)                                  gAAGTGGTGCGGGATAAAAGCGACAACTGCATCATCGTCTGCTCTATCGAAAACTTCGATGCGATGGGCAGCCACACCGGTGACTCCATCACTGTCGCGCCAGCCCAAACGCTGACCGACAAAGAATATCAAAGCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCTGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAAccc                                                                                                                                                                                                    <  1:337261/251‑1 (MQ=255)                                    aGTGGTGCGTGATAAAAACGACAACTGCATCATCGTCTGCTCTATCGAAAACTTCGATGCGATGGGCATCCACACCGGTGACTCCATCACTGTCGCGCCAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCAc                                                                                                                                                                                                  >  1:357474/1‑251 (MQ=255)                                     gtggtgCGTGATAAAAACGGCAACTGCAACATCGTCTGCTCTATCGAAAACTTCGATGCGATGGGCATCCACACCGGTGACTCCATCACTGTCGCGCCAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCAcg                                                                                                                                                                                                 <  2:69927/251‑1 (MQ=255)                                        gtgCGTGATAAAAACGACAACTGCATCATCGTCTGCTCTATCGAAAACTTCGATGCGATGGGCATCCACACCGGTGACTCCATCACTGTCGCGCCAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGCACCCAcgcgg                                                                                                                                                                                              >  2:116562/1‑250 (MQ=255)                                              gATAAAAACGACAACTGCATCATCGTCTGCTCTATCGAAAACTTCGATGCGATGGGCATCCACACCGGTGACTCCATCACTGTCGCGCCAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCg                                                                                                                                                                                        >  1:373962/1‑251 (MQ=255)                                               aTAAAAACGACAACTGCATCATCGTCTGCTCTATCGAAAACTTCGATGCGATGGGCATCCACACCGGTGACTCCATCACTGTCGCGCCAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGt                                                                                                                                                                                       <  1:462870/251‑1 (MQ=255)                                               aTAAAAACGACAACTGCATCATCGTCTGCTCTATCGAAAACTTCGATGCGATGGGCATCCACACCGGTGACTCCATCACTGTCGCGCCAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGATCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGt                                                                                                                                                                                       <  2:103625/251‑1 (MQ=255)                                                 aaaaaCGCCAACTGCATCATCGTCTGCTCTATCGAAAACTTCGATGCGATGGGCATCCACACCGGTGACTCCATCACTGTCGCGCCAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGttc                                                                                                                                                                                     >  1:156443/1‑251 (MQ=255)                                                 aaaaaCGACAACTGCATCATCGTCTGCTCTATCGAAAACTTCGATGCGATGGGCATCCACACCGGTGACTCCATCACTGTCGCGCCAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGttc                                                                                                                                                                                     >  1:514477/1‑251 (MQ=255)                                                 aaaaaCGACAACTGCATCATCGTCTGCTCTATCGAAAACTTCGATGCGATGCGCATCCACACCGATGACTCCATCACTGTTGCGACAGCCCAAACGCTGACCGACAAAGACTATCAAATCATGCGTAACGCCTCGATGGCGGTGATGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGAGGTGAATCCGAAAAACGGTCGTATGATTGTTATCGAAATGAACCCACGCGTGTCCCGttc                                                                                                                                                                                     >  2:581834/1‑251 (MQ=255)                                                    aaCGACAACTGCATCATCGTCTGCTCTATCGAAAACTTCGATGCGATGGGCATCCACACCGGTGCCTCCATCACTGTCGCGCCAGACCAAACGCTGACCGACAAAGAATATCAAAGCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTGCCAACTTTCAGAGTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGttcttc                                                                                                                                                                                  <  2:523124/251‑1 (MQ=255)                                                    aaCGACAACTGCATCATCGTCTGCTCTATCGAAAACTTCGATGCGATGGGCATCCACACCGGTGACTCCATCACTGTCGCGCCAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGGGCTGCGTGAAATCGGCGTGGAAACCGGTGGTGCCAACGTGCAGTTGGCGGTGAATCCGAAAAACGGGCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGttcttc                                                                                                                                                                                  <  1:574738/251‑1 (MQ=255)                                                      cGACAACTGCATCATCGTCTGCTCTATCGAAAACTTCGATGCGATGGGCATCCACACCGGTGACTCCATCACTGTCGCGCCAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCgg                                                                                                                                                                                >  1:580588/1‑251 (MQ=255)                                                            cTGCATCATCGTCTGCTCTATCGAAAACTTCGATGCGATGGGCATCCACACCGGTGACTCCATCACTGTCGCGCCAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTgg                                                                                                                                                                          >  2:179109/1‑251 (MQ=255)                                                              gCATCATCGTCTGCTCTATCGAAAACTTCGATGCGATGGGCATCCACACCGGTGACTCCATCACTGTCGCGCCAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGc                                                                                                                                                                         <  1:129944/250‑1 (MQ=255)                                                                     cGTCTGCTCTATCGAAAACTTCGATGCGATGGGCATCCACACCGGTGACTCCATCACTGTCGCGCCAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGaa                                                                                                                                                                   >  1:83582/1‑249 (MQ=255)                                                                          gCTCTATCGAAAACTTCGATGCGATGGGCATCCACACCGGTGACTCCATCACTGTCGCGCCAGCCCAAACGCTGACCGACAAAGAATATCACATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGAcc                                                                                                                                                            >  2:513318/1‑251 (MQ=255)                                                                           ctctATCGAAAACTTCGATGCGATGGGCATCCACACCGGTGACTCCATCACTGTCGCGCCAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTACTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCGACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCg                                                                                                                                                           >  1:453642/1‑251 (MQ=255)                                                                             cgaTCGAAAACTTCGATGCGATGGGCATCCACACCGGTGACTCCATCACTGTCGCGCCAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAAGGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGt                                                                                                                                                         <  2:42698/249‑1 (MQ=255)                                                                              tATCGAAAACTTCGATGCGATGGGCATCCACACCGGTGACTCCATCACTGTCGCGCCAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGtt                                                                                                                                                        >  1:178473/1‑251 (MQ=255)                                                                               aTCGAAAACTTCGATGCGATGGGCATCCACACCGGTGACTCCATCACTGTCGCGCCAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGtt                                                                                                                                                        <  1:559699/250‑1 (MQ=255)                                                                               aTCGAAAACTTCGATGCGATGGGCATCCACACCGGTGACTCCATCACAGTCGCGCCAGCCCAAACGCGGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGGGGTTCCAACGTTCAGTGTGCGGTGAATCCGAAAAACGGGCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTGCGTCGGCGCTGGCGTCGAAAGCGACCGGttt                                                                                                                                                       <  2:92760/251‑1 (MQ=255)                                                                                               cgatgGGCATCCACACCGGTGACTCCATCACTGTCGCGCCAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGGGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTg                                                                                                                                       >  2:408499/1‑251 (MQ=255)                                                                                                    ggCATCCACACCGGTGACTCCATCACTGTCGCGCCAGCCCAAACGCTGACCGCAAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTggcggg                                                                                                                                  >  1:40447/1‑250 (MQ=255)                                                                                                           acacCGGTGACTCCATCACTGTCGCGCCAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGAGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGaaaatg                                                                                                                           >  1:339555/1‑248 (MQ=255)                                                                                                            cacCGGTGACTCCATCACTGTCGCGCCAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTgg                                                                                                                          >  1:274725/1‑251 (MQ=255)                                                                                                                       ccATCACTGTCGCGCCAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGG‑TGGGtt                                                                                                                <  1:116562/249‑1 (MQ=255)                                                                                                                         aTCACTGTCGCGCCAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCGCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGG‑TGGGTTacac                                                                                                            <  2:102183/251‑1 (MQ=255)                                                                                                                         aTCACTGTCGCGCCAGCACAAACGCTGACCGGCCAAGAATATCAAATCATGCGTAACGCCTCGCTGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTACCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTACAGTGGCGGCGGAACTGGCGG‑TGGGGTactc                                                                                                            >  2:35437/1‑249 (MQ=255)                                                                                                                             cTGTCGCGCCAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGACGGTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGGCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCGGGCGTCGAAAGCGACCGGTTGCCCGATTGCGATATTGGCGGCGAAACTGGCGG‑TGGGTTacac                                                                                                            <  2:186065/247‑1 (MQ=255)                                                                                                                                 cgcgCCAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGG‑TGGGTTACACCCTcgac                                                                                                     <  2:314333/250‑1 (MQ=255)                                                                                                                                    gcCAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCCACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAAATGGCgggcggggatacacccgacgaac                                                                                                  >  1:369622/1‑230 (MQ=255)                                                                                                                                      catcccAAACGCTGACCGACAAAGAATATCAAATCACGCGTAACGCCTCGATGGCGGGTCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTAACGAAATGAACCCACGCGTATCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGG‑TTGGTTACTCCCTCGACGCACtg                                                                                               <  2:547186/248‑1 (MQ=255)                                                                                                                                                   gACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCTCGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGG‑TGGGTTACACCCTCGACGAACTGATGAACGACATc                                                                                   <  1:347053/250‑1 (MQ=255)                                                                                                                                                   gACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGG‑TGGGTTACACCCTCGACGAACTGATGAACGACATCa                                                                                  <  2:33268/251‑1 (MQ=255)                                                                                                                                                    aCCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGTCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGCGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGGTTCCCGATTGCTAAAGTGGCGGCGGAACAGGCGGGTGGGTTAC‑CCCTcgccgcacatgtgggctcacaccc                                                                                 >  2:506097/1‑229 (MQ=255)                                                                                                                                                      cGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGG‑TGGGGTACACCCCCCACGCACTGATGGACGACATCCCTg                                                                               >  1:93381/1‑251 (MQ=255)                                                                                                                                                                      aTCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGG‑TGGGTTACACCCTcg                                                                                                       <  1:375319/211‑1 (MQ=255)                                                                                                                                                                         aTGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGG‑TGGGTTACACCCTCGACGAACTGATGAACGACATCGCTGGCGGACGTACTCCGGcctc                                                            <  1:179109/251‑1 (MQ=255)                                                                                                                                                                               aaCGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGG‑TGGGGTACACCCTCGACGAACTGATGAACGACATCACTGGCggggctactcccggctcctttgg                                                      >  1:142960/1‑230 (MQ=255)                                                                                                                                                                                        aTGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGG‑TGGGTTACACCCTCGACGAACTGATGAACGACATCACTGGCGGACGTACTCCGGCCTCCTTCGAGCCGTCCAt                                             <  2:573654/251‑1 (MQ=255)                                                                                                                                                                                                        atgtCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGG‑TGGGTTACACCCTCGACGAACTGATGAACGACATCACTGGCGGACGTACTCCGGCCTCCTGCGAGCCGTCCATCGACTATGTGGTTACt                             <  2:125807/248‑1 (MQ=255)                                                                                                                                                                                                          aTCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGG‑TGGGTTACACCCTCGACGAACTGATGAACGACATCACTGGCGGACGTACTCCGGCCTCCTTCGAGCCGTCCATCGACTATGTGGTTACTaa                           <  1:503480/251‑1 (MQ=255)                                                                                                                                                                                                                  tGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGG‑t                                                                                                                     >  2:211498/1‑153 (MQ=255)                                                                                                                                                                                                                     aaCCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGG‑TGGGTTACACCCTCGACGAACTGATGAACGACATCACTGGCGGACGTACTCCGGCCTCCTTCGAGCCGTCCATCGACTATGTGGTTACTAAAATTCCTCGCt                <  1:205077/251‑1 (MQ=255)                                                                                                                                                                                                                      aCCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGTTAAAGTGGCGGCGAAACTGGCGG‑TGGGTTACACCCTCGACGAACTGATGAACGACATCACTGGCGGACGTACTCCGGCCTCCTTCGAGCCGTCCATCGACTATGTGGTTACTAAAATTCCTCGCtt               <  1:236090/251‑1 (MQ=255)                                                                                                                                                                                                                            ggtTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGG‑GGGGGTACCACCTCGACGAACTGATGGAAGACATCACTGGCGGGAGTACTCCGGGCCCCTTCGAGCCGTCCATCGCCTATGTGGTTACTTAAATTCCTCGCTTTAAcat         >  1:585485/1‑249 (MQ=255)                                                                                                                                                                                                                             gtTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGGCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGG‑TGGGTTACACCCTCGACGAACTGATGAACGACATCACTGGCGGACGTACTCCGGCCTCCTTCGAGCCGTCCATCGACTATGTGGTTACTAAAATTCCTCGCTTCAACTTc        <  2:373962/251‑1 (MQ=255)                                                                                                                                                                                                                                   aCGTTCAGTGTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGG‑TGGGTTACACCCTCGACGAACTGATGAACGACATCACTGGCGGACGTACTCCGGCCTCCTTCGAGCCGTCCATCGACTATGTGGTTACTAAAATTCCTCGCTTCAACTTCGaaaaa  <  2:157764/251‑1 (MQ=255)                                                                                                                                                                                                                                        cAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGG‑TGGGTTACACCCTCGACGAACTGATGAACGACATCACTGGCGGACGTACTCCGGCCTCCTTCGAGCCGTCCATCGACTATGTGGTTACTAAAATTCCTCGCTTc              <  1:262699/234‑1 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                         GAGTCGCTGATCGGCTGGAAAGAGTACGAGATGGAAGTGGTGCGTGATAAAAACGACAACTGCATCATCGTCTGCTCTATCGAAAACTTCGATGCGATGGGCATCCACACCGGTGACTCCATCACTGTCGCGCCAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGG‑TGGGTTACACCCTCGACGAACTGATGAACGACATCACTGGCGGACGTACTCCGGCCTCCTTCGAGCCGTCCATCGACTATGTGGTTACTAAAATTCCTCGCTTCAACTTCGAAAAA  >  NC_000913/31438‑31914

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑