Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,235,755	C→T	S496N (AGC→AAC)	ycgB ←	PF04293 family protein YcgB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,235,755	0	C	T	100.0%	130.1 / NA	42	S496N (AGC→AAC)	ycgB	PF04293 family protein YcgB
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (24/18);  total (24/18)

CGGCGTTGTGCAGTGAAGGCGCGCACGATCAGGTGTACGAAACAGTGCGTCGCTATGGGCATGAGAGTGGCGAGATTTGGCACCGGATGCAGAAAAATCTGCCGGGTGATTTAGCCATTCAGGGGCGATAATCCCTTCCGTTTAAAGAGCAAACCCCTCAAACGAGGGGTTTTTTGTTGTTTTTACAGATTTCCCATTCTTGGCGGGCAACGTTCCAGCAACTCGATGCTGCCGTCTTCGTTTTGCTGTTCGAGCATCACATCAAATCCCCACAGGCGATGCACATGCTTCAGGACTTCTTTGCGCCCCCGATCCAGCGGTGCGCGATTATGTGGAATATAACGCAGCGTCAGCGAACGGTCGCCGCGCAAATCCACGTTCCAGATCTGAATATTCGGCTCCAGATTACTTAAGTTATATTGCGACGATAACCGGTTACGGATCTCCCGATAACCTTCTTCATTATGAATAGCGG  >  NC_000913/1235527‑1236001                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                       cGGCGTTGTGCAGTGAAGGCGCGCACGATCAGGTGTACGAAACAGTGCGTCGCTATGGGCATGAGAGTGGCGAGATTTGGCACCGGATGCAGAAAAATCTGCCGGGTGATTTAGCCATTCAGGGGCGATAATCCCTTCCGTTTAAAGAGCAAACCCCTCAAACGAGGGGTTTTTTGTTGTTTTTACAGATTTCCCATTCTTGGCGGGCAACGTTCCAGCAACTCGATGTTGCCGTCTTCGTTTTGCTGTTc                                                                                                                                                                                                                                  <  1:5812/251‑1 (MQ=255) cGGCGTTGTGCAGTGAAGGCGCGCACGAGCAGGTGTACGAAACAGTGCGTCGCTATGGGCACGAGAGTGGCGAGATGTGTCACCGGAGGCAGAAAAATCTGCCGGGTGATTTAGCCATTCAGGGGCGATAATCCCTTCCGTTTAAAGAGCAAACCCCTCAAACGGGGGGTTGTTTGTTGGTTTGTCAGATTTCCCATGCTTGGCGGGCAACGTTCCAGCAACTCGATGTTGCCGTCTTCGTTTTGCTGTTc                                                                                                                                                                                                                                  <  1:87476/251‑1 (MQ=255)                aaGGCGCGCACGATCAGGTGTACGAAACAGTGCGTCGCTATGGGCATGAGAGTGGCGAGATTTGGCACCGGATGCAGAAAAATCTGCCGGGTGATTTAGCCATTCAGGGGCGATAATCCCTTCCGTTTAAAGAGCAAACCCCTCAAACGAGGGGTTTTTTGTTGTTTTTACAGATTTCCCATTCTTGGCGGGCAACGTTCCAGCAACTCGATGTTGCCGTCTTCGTTTTGCTGTTCGAGCATCACATCaaa                                                                                                                                                                                                                   <  2:203417/251‑1 (MQ=255)                      cgtgcGATCAGGTGGACGGACCAGTGCGACGCTATGGTCATGAGAGTGGCGAGTTTTGGCACCGGAAGCAGAAAAATCTGCCGGGTGATTTAGCCATTCAGGGGCGATAATCCCTGCCGTTTAAAGAGCAAACCGCTCAAACGAGTGGTTTGTTGTTGTTTCTACAGATTTACCATTCTTGGCGGGCAACTGTCCAGCAACGCGATGTTGCCGTCTTCGTTTGGCTGTTCGAGCAGCGCATCAAAGCCCca                                                                                                                                                                                                             <  2:494627/247‑1 (MQ=255)                                                cGTCGCTATGGGCATGAGAGTGGCGAGATTTGGCACCGGATGCAGAAAAATCTGCCGGGTGATTTAGCCATTCAGGGGCGATAATCCCTTCCGTTTAAAGAGCAAACCCCTCAAACGAGGGGTTTTTTGTTGTTTTTACAGATTTCCCATTCTTGGCGGGCAACGTTCCAGCAACTCGATGTTGCCGTCTTCGTTTTGCTGTTCGAGCATCACATCAAATCCCCACAGGCGATTCACATGCTTCAGGActt                                                                                                                                                                                   >  1:402798/1‑251 (MQ=255)                                                        tGGGCATGAGAGTGGCGAGATTTGGCACCGGATGCAGAAAAATCTGCCGGGTGATTTAGCCATTCAGGGGCGATAATCCCTTCCGTTTAAAGAGCAAACCCCTCAAACGAGGGGTTTTTTGTTGTTTTTACAGATTTCCCATTCTTGGCGGGCAACGTTCCAGCAACTCGATGTTGCCGTCTTCGTTTTGCTGTTCGAGCATCACATCAAATCCCCACAGGCGATGCACATGCTTCAGGACTTCTTTgcgc                                                                                                                                                                           >  1:227866/1‑251 (MQ=255)                                                                        gagaTTTGGCACCGGATGCAGAAAAATCTGCCGGGAGATTTAGCCATTCAGGGGCGATAATCCCTTCCGTTTAAAGAGCAAACCCCTCAAACGAGGGGTTTTTTGTTGTTTTTACAGATTTCCCATTCTTGGCGGGCAACGTTCCAGCAACTCGATGTTGCCGTCTTCGTTTTGCTGTTCGAGCATCACATCAAATCCCCACAGGCGATGCACATGCTTCAGGACTTCTTTGCGCCCCCGATCCCGCGGTg                                                                                                                                                           >  2:558765/1‑251 (MQ=255)                                                                                   ccGGATGCAGAAAAATCTGCCGGGTGATTTAGCCATTCAGGGGCGATAATCCCTTCCGTTTAAAGAGCAAACCCCTCCAACGAGGGGTTTTTTGTTGTTTTTACAGATTTCCCATTCTTGGCGGGCAACGTTCCAGCAACACGATGTTGCCGTCTTCGTTTTGCTGTTCGAGCATCACATCAAATCCCCACAGGCGATGCACATGCTTCAGGACTTCTTTGCGCCCCCGATCCAGCGGCGCGCGATTAtgt                                                                                                                                                >  1:281534/1‑251 (MQ=255)                                                                                           aGAAAAATCTGCCGGGTGATTTAGCCATTCAGGGGCGATAATCCCTTCCGTTTAAAGAGCAAACCCCTCAAACGAGGGGTTTTTTGTTGTTTTTACAGATTTCCCATTCTTGGCGGGCAACGTTCCAGCAACTCGATGTTGCCGTCTTCGTTTTGCTGTTCGAGCATCACATCAAATCCCCACAGGCGATGCACATGCTTCAGGACTTCTTTGCGCCCCCGATCCAGCGGTGCGCGATTATGTGGAatat                                                                                                                                         >  1:553813/1‑250 (MQ=255)                                                                                             aaaaaTCTGCCGGGTGATTTAGCCATTCAGGGGCGATAATCCCTTCCGTTTAAAGAGCAAACCCCTCAAACGAGGGGTTTTTTGTTGTTTTTACAGATTTCCCATTCTTGGCGGGCAACGTTCCAGCAACTCGATGTTGCCGTCTTCGTTTTGCTGTTCGAGCATCACATCAAATCCCCACAGGCGATGCACATGCTTCAGGACTTCTTTGCGCCCCCGATCCAGCGGTGCGCGATTATGTGGAATATAAc                                                                                                                                      <  2:105764/251‑1 (MQ=255)                                                                                                        gggTGATTTAGCCATTCACGGGCGCTAATCCCTTCCGTTTAAAGAGCAAACCCCTCAAACGAGGGGTTTTTTGTTGTTTTTACAGATTTCACATTCTTGGCGGGCAACGTTCCAGCAACTCGATGTTGCCGTCTTCGTTTTGCTGTTCGGGCATAACATCAAATCCCCACCGGCGATGCACATGCTTAAGGACTTCTTTGCGCCCCCGATCCAGCGGTGCGCGATTATGTGGAATATAACGCAGCGTCAGc                                                                                                                           >  2:355626/1‑251 (MQ=255)                                                                                                                     aTTCAGGGGCGATAATCCCTTCCGTTTAAAGAGCAAACCCCTCAAACGAGGGGTTTTTTGTTGTTTTTACAGATTTCCCATTCTTGGCGGGCAACGTTCCAGCAACTCGATGTTGCCGTCTTCGTTTTGCTGTTCGAGCATCACATCAAATCCCCACAGGCGATGCACATGCTTCAGGACTTCTTTGCGCCCCCGATCCAGCGGTGCGCGATTATGTGGAATATAACGCAGCGTCAGCGAACGGTcgccgc                                                                                                              <  1:123978/251‑1 (MQ=255)                                                                                                                           gggCGATAATCCCTTCCGTTTAAAGAGCAAACCCCTCAAACGAGGGGTTTTTTGTTGTTTTTACAGATTTCCCATTCTTGGCGGGCAACGTTCCAGCAACTCGATGTTGCCGTCTTCGTTTTGCTGTTCGAGCATCACATCAAATCCCCACAGGCGATGCACATGCTTCAGGACTTCTTTGCGCCCCCGATCCAGCGGTGCGCGATTATGTGGAATATAACGCAGCGTCAGCGAACGGTCGCCGCGCAAAt                                                                                                        >  1:319097/1‑251 (MQ=255)                                                                                                                            ggCGATAATCCCTTCCGTTTAAAGAGCAAACCCCTCAAACGAGGGGTTTTTTGTTGTGTTTACAGATTTCCCATTCTTGGCGGGCAACGGTCCAGCAACTCGATGTTGCCGTCTTCGTTTTGCTGTTCGAGCATCACATCAAATCCCCACAGGCGATGCACATGCTTCAGGACTTCTTTGCGCCCCCGATCCAGCGGTGCGCGATTATGTGGAATATAACGCAGCGTCAGCGAACGGTCGCCGCTCAAATc                                                                                                       <  1:551613/251‑1 (MQ=255)                                                                                                                                aTAATCCCTTCCGTTTAAAGAGCAAACCCCTCACACGAGGGGTTTTTTGTTGTTTTTCCAGCTTTCCCATTCTTGGCGGGCAACGTTCCAGCAACTCGATGTTGCCGTCTTCGTTTTGCTGTTCGAGCATCCCATCAAATCCCCACAGGCGATGCACATGCTTCAGGACTTCTTTGCGCCCCCGATCCCGCGGTgc                                                                                                                                                          >  2:541305/1‑196 (MQ=255)                                                                                                                                aTAATCCCTTCCGTTTAAAGAGCAAACCCCTCAAACGAGGGGTTTTTTGTTGTTTTTACAGATTTCCCATTCTTGGCGGGCAACGTTCCAGCAACTCGATGTTGCCGTCTTCGTTTTGCTGTTCGAGCATCACATCAAATCCCCACAGGCGATGCACATGCTTCAGGACTTCTTTGCGCCCCCGATCCAGCGGTgc                                                                                                                                                          <  1:541305/196‑1 (MQ=255)                                                                                                                                   aTCCCTTCCGTTTAAAGAGCAAACCCCTCAAACGAGGGGTTTTTTGTTGTTTTTACAGATTTCCCATTCTTGGCGGGCAACGTTCCAGCAACTCGATGTTGCCGTCTTCGTTTTGCTGTTCGAGCATCACATCAAATCCCCACAGGCGATGCACATGCTTCAGGACTTCTTTGCGCCCCCg                                                                                                                                                                      >  1:581597/1‑181 (MQ=255)                                                                                                                                   aTCCCTTCCGTTTAAAGAGCAAACCCCTCAAACGAGGGGTTTTTTGTTGTTTTTACAGATTTCCCATTCTTGGCGGGCAACGTTCCAGCAACTCGATGTTGCCGTCTTCGTTTTGCTGTTCGAGCATCACATCAAATCCCCACAGGCGATGCACATGCTTCAGGACTTCTTTGCGCCCCCg                                                                                                                                                                      <  2:581597/181‑1 (MQ=255)                                                                                                                                      ccTTCCGTTTAAAGAGCAAACCCCTCAACCGAGGGGTTTTTTGTTGTTTTTACAGATTTCCCATTCTTGGCGGGCAACGTTCCCGCAACTCGCTGTTGCCGTCTTCGTTTTGCTGTTCGAGCATCACATCAAATCCCCACAGGCGATGCACATGCTTCAGGACTTCTTTGCGCCCACGATCCAGCGGTGCGCGATTATGTGGAATATCACGCAGCGTCAGCGAACGGTCGCCGCGCAAATCCACATTcc                                                                                               >  2:566917/1‑249 (MQ=255)                                                                                                                                          ccGTTTAAAGAGCAAACCCCTCAAACGAGGGGTTTTTTGTTGTTTTTACAGATTTCCCATTCTTGGCGGGCAACGTTCCAGCAACTCGATGTTGCCGTCTTCGTTTTGCTGTTCGAGCATCACATCAAATCCCCACAGGCGATGCACATGCTTCAGGACTTCTTTGCGCCCCCGATCCAGCGGTGCGCGATTATGTGGAATATAACGCAGCGTCAGCGAACGGTCGCCGCGCAAATCCACGTTCCAGATCt                                                                                         <  1:235617/251‑1 (MQ=255)                                                                                                                                               tAAAGAGCAAACCCCTCAAACGAGGGGTTTTTTGTTGTTTTTACAGATTTCCCATTCTTGGCGGGCAACGTTCCAGCAACTCGATGTTGCCGTCTTCGTTTTGCTGTTCGAGCATCACATCAAATCCCCACAGGCGATGCACATGCTTCAGGACTTCTTTGCGCCCCCGATCCAGCGGTGCGCGATTATGTGGAATATAACGCAGCGTCAGCGAACGGTCGCCGCGCAAATCCCCGTTCCAGATCTGact                                                                                     >  2:484880/1‑248 (MQ=255)                                                                                                                                                      cAAACCCCTCAAATGAGGGGTTTTTTGTTGTTTTTACAGATTTCCCATTCTTGGCGGGCAACGTTCCAGCAACTCGATGTTGCCGTCTTCGTTTTGCTGTTCGAGCATCACATCAAATCCCCACAGGCGATGCACATGCTTCAGGACTTCTTTGCGCCCCCGATCCAGCGGTGCGCGATTATGTGGAATATAACGCAGCGTCAGCGAACGGTCGCCGCGCAAATCCACGTTCCAGATCTGAATATTCGGCt                                                                             <  1:578867/251‑1 (MQ=255)                                                                                                                                                      cAAACCCCTCAAACGAGGGGTTTTTTGTTGTTTTTACAGATTTCCCATTCTTGGCGGGCAACGTTCCAGCAACTCGATGTTGCCGTCTTCGTTTTGCTGTTCGAGCATCACATCAAATCCCCACAGGCGATGCACATGCTTCAGGACTTCTTTGCGCCCCCGATCCAGCGGTGCGCGATTATGTGGAATATAACGCAGCGTCAGCGAACGGTCGCCGCGCAAATCCACGTTCCAGATCTGAATATTCGGCt                                                                             >  1:243008/1‑251 (MQ=255)                                                                                                                                                           cccTCAAACGAGGGGTTTTTTGTTGTTTTTACAGATTTCCCATTCTTGGCGGGCAACGTTCCAGCAACTCGATGTTGCCGTCTTCGTTTTGCTGTTCGAGCATCACATCAAATCCCCACAGGCGATGCACATGCTTCAGGACTTCTTTGCGCCCCCGATCCAGCGGTGCGCGATTATGTGGAATATAACGCAGCGTCAGCGAACGGTCGCCGCGCAAATCCACGTTCCAGATCTGAATATTCGGCTCCAGa                                                                        <  2:319097/251‑1 (MQ=255)                                                                                                                                                            ccTCAAACGAGGGGTTTTTTGTTGTTTTTACAGATTTCCCATTCTTGGCGGGAAAAGATCCAGCAACTCGTTGTTGCCGTCTTCGTTTTGCTGTTCGAGAATCACATCAAATCACCACAGGCGATGCACATGCTTAAGGACTTCTATACGCCCCCGATCCAACGGTGCGCGATTATGTGGAATATACCGCCGCGTAAGCAAAAGGTCGCCGAGCAAATACACGTTCCAGCTCTGAATATTCgaatccaggt                                                                       >  1:52039/1‑242 (MQ=255)                                                                                                                                                              tCAAACGAGGGGTTTTTTGTTGTTTTTACAGATTTCCCATTCTTGGCGGGCAACGTTCCAGCAACTCGATGTTGCCGTCTTCGTTTTGCTGTTCGAGCATCACATCAAATCCCCACAGGCGATGCACATGCTTCAGGACTTCTTTGCGCCCCCGATCCAGCGGTGCGCGATTATGTGGAATATAACGCAGCGTCAGCGAACGGTCGCCGCGCAAATCCACGTTCCAGATCTGAATATTCGGCTCCAGAtt                                                                      >  1:164932/1‑250 (MQ=255)                                                                                                                                                                 aaCGAGGGGTTTTTTGTTGTTTTTACAGATTTCCCATTCTTGGCGGGCAACGTTCCAGCAACTCGATGTTGCCGTCTTCGTTTTGCTGTTCGAGCATCACATCAAATCCCCACAGGCGATGCACATGCTTCAGGACTTCTTTGCGCCCCCGATCCAGCGGTGCGCGATTATGTGGAATATAACGCAGCGTCAGCGAACGGTCGCCGCGCAAATCCACGTTCCAGATCTGAATATTCGGCTCCAGATTACtt                                                                  >  2:151983/1‑251 (MQ=255)                                                                                                                                                                    gAGGGGTTTTTTGTTGTTTTTACAGATTTCCCATTCTTGGCGGGCAACGTTCCAGCAACTCGCTGTTGCCGTCTTCGTTTTGCTGTTCGAGCATCACATCAAATCCCCACAGGCGCTGCACATGCTTCAGGACTTCTTTGCGCCCCCGATCCAGCGGTGCGCGATTATGTGGAATATAACGCAGCGTCAGCGAACGGTCGCCTCGCAAATCCACGTTCCAGATCTGAATATTCGGCTCCAGATTACTTa                                                                 >  2:259318/1‑249 (MQ=255)                                                                                                                                                                        ggTTTTTTGTTGTTTTTACAGATTTCCCATTCTTGGCGGGCAACGTTCCAGCAACTCGATGTTGCCGTCTTCGTTTTGCTGTTCGAGCATCACATCAAATCCCCACAGGCGATGCACATGCTTCAGGACTTCTTTGCGCCCCCGATCCAGCGGTGCGCGATTATGGGGAATATAACGCAGCGTCAGCGAACGGTCGCCGCGCCAATCCACGTTCCAGATCTGAATATTCGGCTCCAGATTACTTAAGTtat                                                           >  1:469753/1‑251 (MQ=255)                                                                                                                                                                              ttgttgTTTTAACAGATTTCCCATTCTTGGCGGGCAACGTTCCAGCAACTCTATGTTGCCCTATTCGTTTTTCTGTTCCAGCCTCACATCAAATCCCCACAGGCGATGCACATGCTTCAGGACTTCTTTGCGCCCCCGATCCAGCGGTGAGCGATTATGTGGAATATAACGCAGCGTCAGCGAACGGTCGCCGCGCAAATCCACGTTCCAGATCTGAATATTCGGCTCCAAATTACTTAAGTTATAtttcg                                                     >  1:103715/1‑248 (MQ=255)                                                                                                                                                                                 ttgttTTTACAGATTTCCCATTCTTGACGGGCAACGTTCCAGCAACTCGATGTTGCCGTCTTCGTTTTGCTGTTCGAGCATCACATCAAATCCCCACAGGCGATGCACATGCTTCAGGACTTCTTTGCGCCCCCGATCCAGCGGTGCGCGATTATGTGGAATATAACGCAGCGTCAGCGAACGGTCGCCGCGCAAATCCACGTTCCAGCTCTGAATATTAGGCTCCAGATTACTTAAGATATATTGcgacg                                                  >  1:314632/1‑251 (MQ=255)                                                                                                                                                                                  tgttTTTACAGTTTTCCCATTCTTGGCGGGCAACGTTCCAGCAACTCGATGTTGCCGTCTTCGTTTTGCTGTTCGAGCATCACATCAAATCCCCACAGGCGATGCACATGCTTCAGGACTTCTTTGCGCCCCCGATCCAGCGGTGCGCGATTATGTGGAATATAACGCAGCGTCAGCGAACGGTCGCCGCGCAAATCCAAGTTCCAGATCTGAATATTCGGCTCCAGATTACTTAATTTATATTGcgacgt                                                 >  2:569521/1‑250 (MQ=255)                                                                                                                                                                                           aGATTTCCCATTCTTGGCGGGCAACGTTCCAGCAACTCGATGTTGCCGTCTTCGTTTTGCTGTTCGAGCATCACATCAAATCCCCACAGGCGATGCACATGCTTCAGGACTTCTTTGCGCCCCCGATCCAGCGGTGCGCGATTATGTGGAATATAACGCAGCGTCAGCGAACGGTCGCCGCGCAAATCCACGTTCCAGATCTGAATATTCGGCGCCAGATTACTTAAGTTATATTGCGACGATAACCGGtt                                        <  2:243008/251‑1 (MQ=255)                                                                                                                                                                                                 cccATTCTTGGCGGGCAACGTTCCAGCAACTCGATGTTGCCGTCTTCGTTTTGCTGTTCGAGCATCACATCAAATCCCCACAGGCGATGCACATGCTTCAGGACTTCTTTGCGCCCCCGATCCAGCGGTGCGCGATTATGTGGAATATAACGCAGCGTCAGCGAACGGTCGCCGCGCAAATCCACGTTCCAGATCTGAATATTCGGCTCCAGATTACTTAAGTTATATTGCGACGATAACCGGTTACGGAt                                  <  1:357534/251‑1 (MQ=255)                                                                                                                                                                                                         tGGCGGGCAACGTTCCAGCAACTCGATGTTGCCGTCTTCGGTTTGCTGTTCGAGCATCACATCAAATCCCCACAGGCGATGCACATGCTTCAGGACTTCTTTGCGCCCCCGATCCAGCGGTGCGCGATTATGTGGAATATAACGCAGCGTCAGCGAACGGTCGCCGCGCAAATCCACGTTCCAGATCTGAATATTCGGCTCCAGATTACTTAAGTGATATTGCGACGATAACCGGTTACGGATCTCCCGa                           <  2:480526/250‑1 (MQ=255)                                                                                                                                                                                                           gCGGGCAACGTTCCAGCAACTCGATGTTGCCGTCTTCGTTTTGCTGTTCGAGCATCACATCAAATCCCCACAGGCGATGCACATGCTTCAGGACTTCTTTGCGCCCCCGATCCAGCGGTGCGCGATTATGTGGCATATAACGCAGCGTCAGCGAACGGTCGCCGCGCAAATCCACGTTCCAGATCTGAATATTCGGCTCCAGATTACTTAAGTTATATTGCGACGATAACCGGTTACGGATCTCCCGATa                         >  2:588679/1‑250 (MQ=255)                                                                                                                                                                                                                cAACGTTCCAGCAACTCGATGTTGCCGTCTTCGTTTTGCTGTTCGAGCATCACATCAAATCCCCACAGGCGATGCACATGCTTCAGGACTTCTTTGCGCCCCCGATCCAGCGGTGCGCGATTATGTGGAATATAACGCAGCGTCAGCGAACGGTCGCCGCGCAAATCCACGTTCCAGATCTGAATATTCGGCTCCAGATTACTTAAGTTATATTGCGACGAGAACCGGTTACTTATCTCTCGATAACcttc                   <  2:390311/251‑1 (MQ=255)                                                                                                                                                                                                                 aaCGTTCCAGCAACTCGATGTTGCCGTCTTCGTTTTGCTGTTCGAGCATCACATCAAATCCCCACAGGCGATGCACATGCTTCAGGACTTCTTTGCGCCCCCGATCCAGCGGTGCGCGATTATGTGGAATATAACGCAGCGTCAGCGAACGGTCGCCGCGCAAATCCACGTTCCAGATCTGAATATTCGGCTCCAGATTACTTAAGTTATATTGCGACGATAACCGGTTACGGATCTCCCGATAACcttct                  >  2:469903/1‑251 (MQ=255)                                                                                                                                                                                                                  aCGTTCCAGCAACTCGATGTTGCCGTCTTCGTTTTGCTGTTCGAGCATCACATCAAATCCCCACAGGCGATGCCCATGCTTCAGGCCTTCTTTGCGCCCCCGATCCAGCGGTGCGCGATTATGTGGAATATAACGCAGCGTCAGCGAACGGTCGCCGCGCAAATCCACGTTCCAGATCTGAATATTCGGCTCCAGATTACTTAAGTTATATTGCGACGATAACCGGTTACGGATCTCCCGATAACcttctt                 >  2:298811/1‑251 (MQ=255)                                                                                                                                                                                                                  aCGTTCCAGCAACTCGATGTTGCCGTCTTCGTTTTGCTGTTCGAGCATCACATCAAAGCCCCACAGGCGATGCACATGCTTCAGGACTTCTTTGCGCCCCCGATCCAGCGGTGCGCGATTATGTGGAATATAACGCAGCGTCAGCGAACGGTCGCCGCGCAAATCCACGTTCCAGATCTGAATATTCGGCTCCAGATTACTTAAGTTATATTGCGACGATAACCGGTTACGGATCTCCCGATAACcttctt                 <  2:553813/251‑1 (MQ=255)                                                                                                                                                                                                                      tCCAGCAACTCGATGTTGCCGACTTCGTCTTGCTGTTCGAGCATCACATCAAATCCCCACAGGCGATGCACATGCTTCAGGACTTCTTTGCGCCCCCGCTCCAGCGGTGCGCGATTATGTGGAATATAACGCAGCGTCAGCGAACGGTCGCCGCGCAAATCCACGTTCCAGATCTGAATATTCGGCTCCAGATTACTTACGTTATATTGCGACGATAACCGGTTACGGATCTCCCGATACCCTTCTTCAtt             >  2:356127/1‑251 (MQ=255)                                                                                                                                                                                                                                 gATGTTGCCGTCTTCGTTTTGCTGTTCGAGCATCACATCAAATCCCCACAGGCGATGCACATGCTTCAGGACTTCTTTGCGCCCCCGATCCAGCGGTGCGCGAGTATGTGGAATATAACGCAGCGTCAGCGAACGGTCGCCGCGCAAATCCACGTTCCAGATCTGAATATTCGGCTCCAGATTACTTAAGTTATATTGCGACGATAACCGGTTACGGATCTCCCGATAACCTTCGTCATTATGAATAGCgg  <  2:314632/251‑1 (MQ=255)                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                       CGGCGTTGTGCAGTGAAGGCGCGCACGATCAGGTGTACGAAACAGTGCGTCGCTATGGGCATGAGAGTGGCGAGATTTGGCACCGGATGCAGAAAAATCTGCCGGGTGATTTAGCCATTCAGGGGCGATAATCCCTTCCGTTTAAAGAGCAAACCCCTCAAACGAGGGGTTTTTTGTTGTTTTTACAGATTTCCCATTCTTGGCGGGCAACGTTCCAGCAACTCGATGCTGCCGTCTTCGTTTTGCTGTTCGAGCATCACATCAAATCCCCACAGGCGATGCACATGCTTCAGGACTTCTTTGCGCCCCCGATCCAGCGGTGCGCGATTATGTGGAATATAACGCAGCGTCAGCGAACGGTCGCCGCGCAAATCCACGTTCCAGATCTGAATATTCGGCTCCAGATTACTTAAGTTATATTGCGACGATAACCGGTTACGGATCTCCCGATAACCTTCTTCATTATGAATAGCGG  >  NC_000913/1235527‑1236001

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑