Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,197,637	C→T	G201S (GGT→AGT)	ymfE ←	uncharacterized protein YmfE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,197,637	0	C	T	100.0%	113.0 / NA	33	G201S (GGT→AGT)	ymfE	uncharacterized protein YmfE
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (18/15);  total (18/15)

CGTGGCCATTTTTCTCAATGCTTCTAATATACTCGCAAAGATATCTAGAGGGCATCGTATGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCCGTGCATATTATAGTTCAAAGCAAGTACCATTCAATTAATGTTATTTTTAGTGAAAAATTCTTTTATTTTATCGTCCGGGATTTTTCCGACTTGACTTTCAGTTCTGGGCTTAACTGTCAAAGCAAAGATAATACCACTCACTGAGAATGATAAAATTACAAAAAAGAATAAAGACAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGAAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATTGCTCAT  >  NC_000913/1197406‑1197859                                                                                                                                                                                                                                        |                                                                                                                                                                                                                               cGTGGCCATTTTTCTCAATGCTTCTAATATACTCGCAAAGATATCTAGAGGGCATCGTATGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCCGTGCATATTATAGTTCAAAGCAAGTTCCATTCAATTAATGTTATTTTTAGTGAAAAATTCTTTTATTTTATCGTCCGGGATTTTTCCGACTTGACTTTCAGTTCTGGGCTTAACTGTCAAAGTAAAGCTAATACTTCTCACTGAGAATGATaaa                                                                                                                                                                                                             <  1:448543/251‑1 (MQ=255)      ccATTTTTCTCAATGCTTCTAATATACTCGCAAAGATATCTAGAGGGCATCGTATGAGGATTTGCTGCATTCTCTGAGCGAATATTAACTCCGTGCATATTATAGTTCAAAGCAAGTACCATTCAATTAATGTTATTTTTAGTGAAAAATTCTTTTATTTTATCGTCCGGGATTTTTCCGACTTGACTTTCAGTTCTGGGCTTAACTGTCAAAGCAAAGATAATACTACTCACTGAGAATGATAAAATTAc                                                                                                                                                                                                        >  1:505979/1‑251 (MQ=255)          ttttCTCAATGCTTCTAATATACTCGCAAAGATATCTAGATTGCATCGTATGAGGTTTTGCTGCAGTCTCTGAGCGAATATTAACTCCGTGCATATTATAGTTCAAAGCAAGTACCATTCAATTAATGTTATTTTTAGTGAAAAATTCTTTTATTTTATCGTCCGAGATTTTTCCGACTTGACTTTCAGTTCTGGGCTTAACTGTCAAAGCAAAGATAATACTACGCACTGATAATGATAAAATTACaaa                                                                                                                                                                                                     <  2:341341/250‑1 (MQ=255)                  attcTTCTAATATACTCGCAAAGATATCTAGAGGGCATCGTATGAGGTTTTGCTGCATTCTCTTAGCGAATATTAACTCCGTGCATATTATAGTTCAAAGCAAGTACCATTCAATTAATGTTATTTTTAGTGAAAAATTCTTTTATTTTATCGTCCGGGATTTTTCCGACTTGACTTTCAGTTCTGGGCTTAACTGTCAAAGCAAAGATAATACTACACACTgag                                                                                                                                                                                                                      <  2:517600/222‑1 (MQ=255)                  aTGCTTCTAATATACTCGCAAAGATATCTAGAGGGCATCGTATGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCCGTGCATATTATAGTTCAAAGCAAGTACCATTCAATTAATGTTATTTTTAGTGAAAAATTCTTTTATTTTATCGTCCGGGATTTTTCCGACTTGACTTTCAGTTCTGGGCTTAACTGTCAAAGCAAAGATAATACTACTCACTgag                                                                                                                                                                                                                      >  1:517600/1‑225 (MQ=255)                         tAATATACTCGCAAAGATATCTAGAGGGCATCGTATGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCCGTGCATATTATAGTTCAAAGCAAGTACCATTCAATTAATGTTATTTTTAGTGAAAAATTCTTTTATTTTATCGTCCGGGATTTTTCCGACTTGACTTTCAGTTCTGGGCTTAACTGTCAAAGCAAAGATAATACTACTCACTGAGAATGATAAAATTACAAAAAAGAATAAAGACAGc                                                                                                                                                                                     >  1:437512/1‑251 (MQ=255)                                  cGCAAAGATATCTAGAGGGCAGCGTATGAGATTTTGCTGCATTCTCTGAGCGAATATTAACTCCGTGCATATTATATTTCAAAGCAAGTACCATTCAATTAATGTTTTTTTTAGTGAAAAATTCTTTTATTTTATCGTCCGGGATTTTTCCGACTTGACTTTCAGTTCTGGGCTTAACTGTCAAAGCAAAGATAATACTACTCACTGAGAATGATAAAATTACAAAAAAGAATAAAGACAGCAAAGAACCt                                                                                                                                                                            <  2:446977/251‑1 (MQ=255)                                          tatCTAGAGGGCATCGTATGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCCGTGCATATTATAGTTCAAAGCAAGTACCATTCAATTAATGTTATTTTTAGTGAAAAATTCTTTTATTTTATCGTCCGGGATTTTTCCGACTTGACTTTCAGTTCTGGGCTTAACTGTCAAAGCAAAGATAATACTACTCACTGAGAATGATAAAATTACAAAAAAGAATAAAGACAGCAAAGAACCTTCAACATg                                                                                                                                                                    >  2:227960/1‑251 (MQ=255)                                              cagagGGCATCGTATGATGATTTGAAGCATTCTCTGAGCGAATATTAACTCCGTGCATATTATAGTTCAAAGCAAGTACCATTCAATTAATCTTATTTTTAGTGATAAATTCTTTTATTTTATCGTCCGGGATTTTTCCGACTTGACTTTAAGTTTTTTTCTTAACTGTCAACTCAACGATAATAATACTCCCTGAGAATGATAAAATTACAAAAAAGAATAAAGACAGTAAAGAACCTTCAACATGaaa                                                                                                                                                                 >  2:45266/2‑250 (MQ=255)                                               agagGGCATCGTATGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCCGTGCATATTATAGTTCAAAGCAAGTACCATTCAATTAATGTTATTTTTAGTGAAAAATTCTTTTATTTTATCGTCCGGGATTTTTCCGACTTGACTTTCAGTTCTGGGCTTAACTGTCAAAGCAAAGATAATACTACTCACTGAGAATGATAAAATTACAAAAAAGAATAAAGACAGCAAAGAACCTTCAACATGaaaa                                                                                                                                                                >  2:180616/1‑250 (MQ=255)                                                gagtggaTCGTATGAGGATTTACTGAATTCTCTGAGCGCATACTAACTCCGTGCATATTATAATTCAAAGCAAGTACCATTCAATTAATGTTATTTTTAGTGACAAATTCTTATATTGTATCGTCCGGGATTTTTCCGACTTGATTTTCAGATCTGGGCTTTACTCTCAAAGCAAAGATAATACTACTAATTGAGAATGATAAAATTACAAAAGAGACTAACGAAAGCAAAGAACATTCAACATGaaaact                                                                                                                                                              >  2:525015/7‑249 (MQ=255)                                                 cgggCATCGTATGAGGTTTTGCTGCATTCTCTGCGCGAATATTAACTCCGTGCATATTATAGTTCAAAGCAAGTACCATTCAATTAATGTTATTTTTAGTGAAAAATTCTTTTATTTTATCGTCCGGGATTTTTCCGACTTGACTTTCAGTTCTGGGCTTAACTGTCAAAGCAAAGATAATACTACTCACTGAGAATGATAAAATTACAAAAAAGAATAAAGACAGCAAAGAACCTTCAACATGAAAAat                                                                                                                                                              >  2:285021/2‑250 (MQ=255)                                                  gggCATCGTATGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCCGTGCATATTATAGTTCAAAGCAAGTACCATTCAATTAATGTTATTTTTAGTGAAAAATTCTTTTATTTTATCGTCCGGGATTTTTCCGACTTGACTTTCAGTTCTGGGCTTAACTGTCAAAGCAAAGATAATACTACTCACTGAGAATGATAAAATTACAAAAAAGAATAAAGACAGCAAAGAACCTTCAACATGAAAAatat                                                                                                                                                            >  1:221684/1‑251 (MQ=255)                                                      aTCGTATGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCCGTGCATATTATAGTTCAAAGAAAGTACCATTCACTTAATGTTATTTTTAGTGAAAAATTCTTTTATTTTATCGTCCGGGATTTTTCCGACTTGACTTTCAGTTCTGGGCTTAACTGTCAAAGCACAGATAATACTACTCACTGAGAATGATAAAATTACAAAAAAGAATAAAGACAGCAAAGAACCTTCAACATGAAAAATATCCAt                                                                                                                                                        >  1:317148/1‑251 (MQ=255)                                                        cGTATGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCCGTGCATATTATAGTTCAAAGCAAGTACCATTCAATTAATGTTATTTTTAGTGAAAAATTCTTTTATTTTATCGTCCGGGATTTTTCCGACTTGACTTTCAGTTCTGGGCTTAACTGTCAAAGCAAAGATAATACTACTCACTGAGAATGATAAAATTACAAAAAAGAATAAAGACAGCAAAGAACCTTCAACATGAAAAATATCCAttt                                                                                                                                                      <  2:437512/251‑1 (MQ=255)                                                            tGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCCGTGCATATTATAGTTCAAAGCAAGTACCATTCAATTAATGTTATTTTTAGTGAAAAATTCTTTTATTTTATCGTCCGGGATTTTTCCGACTTGACTTTCAGTTCTGGGCTTAACTGTCAAAGCAAAGATAATACTACTCACTGAGAATGATAAAATTACAAAAAAGAATAAAGACAGCAAAGAACCTTCAACATGAAAAATATCCAtttgttt                                                                                                                                                  >  2:530717/1‑251 (MQ=255)                                                                          aTTCTCTGAGCGAATATTAACTCCGTGCATATTATAGTTCAAAGCAAGTACCATTCAATTAATGTTATTTTTAGTGAAAAATTCTTTTATTTTATCGTCCGGGATTTTTCCGACTTGACTTTCAGTTCTGGGCTTAACTGTCAAAGCAAAGATAATACTACTCACTGAGAATGATAAAATTACAAAAAAGAATAAAGACAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGCAAAAAAAGatta                                                                                                                                    <  1:138874/251‑1 (MQ=255)                                                                                 gAGCGAATATTAACTCCGTGCATATTATAGTTCAAAGCAAGTACCATTCAATTAATGTTATTTTTAGTGAAAAATTCTTTTATTTTATCGTCCGGGATTTTTCCGACTTGACTTTCAGTTCTGGGCTTAACTGTCAAAGCAAAGATAATACTACTCACTGAGAATGATAAAATTACAAAAAAGAATAAAGATAGCAAAGAGCCTTCAGTATGAAAAATATCCATTTGTTTGTAAAAAATGATTATTag                                                                                                                                <  2:573774/248‑1 (MQ=255)                                                                                        tatTAACTCCGTGCATATTCTAGTTCAAAGCAAGTACCATTCAATTACTGTTATTTTTAGTGAAAAATTCTTTTATTTTATCGTCCGGGATTTTTCCGACTTGACTTTCAGTTCTGGGCTTAACTGTCAAAGCAAAGATAATACTACTCACTGAGAATGATAAAATTACAAAAAAGAATAAAGACAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGAAAtt                                                                                                                      >  2:286290/1‑251 (MQ=255)                                                                                         attaacttcgatcATATTATAGTTCATAGTAAGTGCCAATCAAGTAATTGTATTTTTAGTGAAAAATTCTTTTATTTTATCGTCCGGGATTTTTCCGACTTGACTGTCAGTTCTGGGCTTAACTGTCAAAGCAAAGAGAATACTACTCACGGAGAATTATAAAATTACAAGAAAGTATAAAGGCAGCAAAAAACCATTAACATGAAAAATATCCGTTTGTTTGCAAAAAAAGATTGTTAGGATGGAAAtt                                                                                                                      <  2:62874/238‑1 (MQ=255)                                                                                                           ataGTTCAAAGCAAGTACCATTCAATTAATGTTATTTTTAGTGAAAAATTCTTTTATTTTATCGTCCGGGATTTTTCCGACTTGACTTTCAGTTCTGGGCTTAACTGTCAAAGCAAAGATAATACTACTCACTGAGAATGATAAAATTACAAAAAAGAATAAAGACAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGAAATTAATGCAATTATCGAAAAtt                                                                                                   <  1:181206/251‑1 (MQ=255)                                                                                                               ttCAAAGCAAGTACCATTCAATTAATGTTATTTTTAGTGAAAAATTCTTTTATTTTATCGTCCGGGATTTTTCCGACTTGACTTTCAGTTCTGGGCTTAACTGTCAAAGCAAAGATAATACTACTCACTGAGAATGATAAAATTACAAAAAAGAATAAAGACAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGAAATTAATGCAATTATCGAAAATTCaa                                                                                                >  1:399360/1‑250 (MQ=255)                                                                                                                   aaGCAAGTACCATTCAATTAATGTTATTTTTAGTTAAAAATTCTGTTATTTTATCGGCCGTGATTTATCCGACTTGACTTTCAGTTCTGGGCTTAACTGTCAAAGCAAAGATAAGACTACTCACTGAGAATGATAAAATTACAAAAAAGAATAAAGACAGAAAAGAACCTTCAACATGAAAAATATCCTGTTGTTTGCAAAAAAAGATTATGAGGAAGGAAATTAATTCAATGATCTAAGATCCAAAAAat                                                                                           <  2:58467/251‑1 (MQ=255)                                                                                                                              aTTCAATTAATGTTATTTTTAGTGAAACATTCTTTTATTTTCTCGTCAGGGATTTTTTATAATTGACTTTCAGTTCTGGGCATCAATGTCCAAGCAAAGATAATACTACTCACTGATAATGATAAAATTACAAAAAAGAATAACGCCAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGACGGAAATTAATGCTATTATCGAAAATTCAAAAACTATCCAAAAAt                                                                                 >  2:536972/1‑250 (MQ=255)                                                                                                                                              ttttAGTGAAAAATTCTTTTATTTTATCGTCCGGGATTTTTCCAACTTGACTTTCAGTTCTGGGCTTAACTGTCAAAGCAAAGATAATACTACTCACTGAGAATGATAAAATTACAAAAAAGAATAAAGACAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGAAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCa                                                                 <  1:101542/250‑1 (MQ=255)                                                                                                                                                 tAGTGAAAAATTCTTTTATTTTATCGTCCGGGATTTTTCCGACTTGACTTTCAGTTCTGGGCTTAACTGTCAAAGCAAAGATAATACTACTCACTGAGAATGATAAAATTACAAAAAAGAATAAAGACAGCAAAGAACCTTCAACATGAAAAATATCCAtttgtttg                                                                                                                                                 <  1:72039/167‑1 (MQ=255)                                                                                                                                                 tAGTGAAAAATTCTTTTATTTTATCGTCCGGGATTTTTCCGACTTGACTTTCAGTTCTGGGCTTAACTGTCAAAGCAAAGATAATACTACTCACTGAGAATGATAAAATTACAAAAAAGAATAAAGACAGCAAAGAACCTTCAACATGAAAAATATCCAtttgtttg                                                                                                                                                 >  2:72039/1‑167 (MQ=255)                                                                                                                                                                         cGTCCGGGATTTTTCCGACTTGACTTTCAGTTCTGGGCTTAACTGTCAAAGCAAAGATAATACTACTCACTGAGAATGATAAAATTACAAAAAAGAATAAAGACAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGAAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTc                                      <  2:326628/250‑1 (MQ=255)                                                                                                                                                                                      tCCGACTTGACTTTCAGTTCTGGGCTTAACTGTCAAAGCAAAGATAATACTACTCACTGAGAATGATAAAATTACAAAAAAGAATAAAGACAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAggaaggaa                                                                                                                         <  2:272434/154‑1 (MQ=255)                                                                                                                                                                                      tCCGACTTGACTTTCAGTTCTGGGCTTAACTGTCAAAGCAAAGATAATACTACTCACTGAGAATGATAAAATTACAAAAAAGAATAAAGACAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAggaaggaa                                                                                                                         >  1:272434/1‑154 (MQ=255)                                                                                                                                                                                             tgacttTCAGTTCTGGGCTTAACTGTCAAAGCAAAGATAATACTACTCACTGAGAATGATAAAATTACAAAAAAGAATAAAGACAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGAAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGt                  >  1:510257/1‑250 (MQ=255)                                                                                                                                                                                                  ttCAGTTCTGGGCTTAACTGTCAAAGCAAAGATAATACTACTCACTGAGAATGATAAAATTACAAAAAAGAATAAAGACAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGAAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAatat             <  2:311088/250‑1 (MQ=255)                                                                                                                                                                                                            ggCTTAACTGACAAAGCAAAGATAATACTACTCACTGAGAATGATAAAATTACAAAAAAGAATAAAGACAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGAACTTACTGCAATTCTCGTAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCATTTTTTCTTACTTCAGGGTAATATAGATTGCTCAt  >  1:112982/1‑251 (MQ=255)                                                                                                                                                                                                                                        |                                                                                                                                                                                                                               CGTGGCCATTTTTCTCAATGCTTCTAATATACTCGCAAAGATATCTAGAGGGCATCGTATGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCCGTGCATATTATAGTTCAAAGCAAGTACCATTCAATTAATGTTATTTTTAGTGAAAAATTCTTTTATTTTATCGTCCGGGATTTTTCCGACTTGACTTTCAGTTCTGGGCTTAACTGTCAAAGCAAAGATAATACCACTCACTGAGAATGATAAAATTACAAAAAAGAATAAAGACAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGCAAAAAAAGATTATTAGGAAGGAAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATTGCTCAT  >  NC_000913/1197406‑1197859

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑