Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,191,644	C→T	E115K (GAA→AAA)	purB ←	adenylosuccinate lyase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,191,644	0	C	T	100.0%	149.9 / NA	43	E115K (GAA→AAA)	purB	adenylosuccinate lyase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (20/23);  total (20/23)

GCGCTCCATACGGTAGGCGACGTTTGCCATCTCTTTACCGATGGTTGACGGCGTGGCTGGCTGACCGTGGGTACGAGACAGCAGCGGGATATCGCGATACTGAACGGCGAGATCTTTAATGCCATCAATCAGTTGACGCCAGTATGGCAGGATCACTTCATCACGCGCGGTTTTCAGCATTAATGCGTGGGAGAGGTTATTGATATCTTCCGAAGTACAGGCAAAGTGGATGAATTCAGAAACCGCGTGCAGTTCCGGGATCTCCGCCACTTTTTCTTTCAGGAAATACTCAACCGCTTTAACGTCGTGGTTAGTGGTACGCTCGATAGTTTTGATGCGCGCCGCATCTTCTTCGCTGAAACTGGCGACGATTGCATCAAGGTAACCGATTGCGTCGGCAGCAAAAGCAGGAACTTCCTTGATCGCTGCGTGCGCGGCCAGTTTTTGCAGCCAACGTACTTCAACTTGTACACGGAATTT  >  NC_000913/1191408‑1191887                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                    gcgcTCCATACGGTAGGCGACGTTTGCCATCTCTTTACCGATGGTTGACGGCGTGGCTGGCTGACCGTGGGTACGAGACAGCAGCGGGATATCGCGATACTGAACGGCGAGATCTTTAATGCCATCAATCAGTTGACGCCAGTATGGCAGGATCACTTCATCACGCGCGGTTTTCAGCATTAATGCGTGGGAGAGGTTATTGATATCTTCCGAAGTACAGGCAAAGTGGATGAATTTAGAAACCGCGTGCa                                                                                                                                                                                                                                       <  2:424594/251‑1 (MQ=255)                            cATCTCTTAACCGGTGGTTGACGGCTGGGCTGGCTGACCGTGGGTACGAGACAGCAGCGGGATATCGCGATACTGAACGGCGAGATCTTTAAGGCCATCAATCAGTTGACTCCAGTATGGCAGGATCACTTCATCACGCGCGGTTTTCAGCATTAATGCGTGGGAGAGTTTTTTGATATCTGCCTAGGTACAGGCAAAGTGGATGAATTTAGAATCGGCGGGCAGTTCCGGTATCTTCGCCACTTtttctt                                                                                                                                                                                                            <  2:65434/251‑1 (MQ=255)                                    tACCGATTGTTGACGGCGTGGCTGGCTGACCGTGGGTACGAGACAGCGGAGGGATATCGCGATACTGAACGGCGAGATCTTTAATGCCAGCAATCAGTTGACGCCAGTGTGTCAGGATCTCTTCATCACGCGCGGTTTTCAGCATTAATGCGTGGGAGAGGTTATTGATATCTTCCGAAGTACAGGCAAAGTGGATGAATTTAGAAACCGCGTGCAGTTGCGGGCTCTCCGCCACTTTTTCTTTCAGGaa                                                                                                                                                                                                     <  2:177376/250‑1 (MQ=255)                                    tACCGATGGTTGACGGCGGGGCTGGCTGACCGTGGGTACGAGACAGCAGCGGGATATCGCGATACTGAACGGCGAGATCTTTAATGCCATCAATCAGTTGACGCCAGTATGGCAGGATCACTTCATCACGCGCGGTTTGCAGCATTAATGCGTGGGAGAGGTGATTGAGATCTTCCGAAGTACAGGCAAAGTGGATGAATTTAGAAACCGCGTGCAGTTCCGGGATCTCCGCCACTTTTTCTTTCAGGaa                                                                                                                                                                                                     <  2:492190/250‑1 (MQ=255)                                     aCCGATGGTTGACGGCGTGGCTGGCTGACCGTGGGTACGAGACAGCAGCGGGATATCGCGATACTGAACGGCGAGATCTTTAATGCCATCAATCAGTTGACGCCAGTATGGCAGGATCACTTCATCACGCGCGGTTTTCAGCATTAATGCGTGGGAGAGGTTATTGATATCTTCCGAAGTACAGGCAAAGTGGATGAATTTAGAAACCGCGTGCAGTTCCGGGATCTCCGCCACTTTTTCTTTCAGGAAAt                                                                                                                                                                                                   <  1:112866/251‑1 (MQ=255)                                      ccGATGGTTGACGGCGTGGCCGGCTGACCGTGGGTACGAGACAGCAGCGGGATATCGCGATACTGAACGGCGAGATCTTTAATGCCATCAATCAGTTGACGCCAGTATGGCAGGATCACTTCATCACGCGCGGTTTTCAGCATTAATGCGTGGGAGAGGTTATTGATATCTTCCGAAGTACAGGCAAAGTGGATGAATTTAGAAACCGCGTGCAGTTCCGGGATCTCCGCCACTTTTTCTTTCAGGAAAt                                                                                                                                                                                                   >  1:427154/1‑250 (MQ=255)                                          tGGTTGACGGCGTGGCTGGCTGACCGTGGGTACGAGACAGCAGCGGGATATCGCGATACTGAACGGCGAGATCTTTAATGCCAACAATCAGTTGACGCCAGTATGGCAGGATCACTTCATCACGCGCGGTTTTCAGCATTAATGCGTGGGAGAGGTTATTGATATCTTCCGAAGTACAGGCAAAGTGGATGAATTTAGAACCCGCGTGCAGTTCCGGGCTCTCCGCCACTTTTTATTTCAGGAAATACTc                                                                                                                                                                                               >  1:285954/1‑250 (MQ=255)                                           ggTTGACGGCGTGGCTGGCTGACCGTGGGTACGAGAAAGCAGCGGGATATCGCGATACTGATCGGCGAGATCTTTAATGCCATCAATCAGTTGACGCCAGTATGGCAGGATCAATTCATCACGCGCGGTTTTCAGCATTAATGCGTGGGGGAGGTTATTGAAATCTTCCGAAGTACAGGCAAAGTGGATGAATTTAGAAACCGCGTGCAGTTTCGGGATCTCCGCCACTTTTTCTTTCAGGAAATACTCa                                                                                                                                                                                              >  2:566992/1‑250 (MQ=255)                                               gACGGCGTGGCTGGCTGACCGTGGGTACGAGTCAGCAGCGGGCAACCGCGATACTGGACGGCGAGATCTTTAATGCCCTCAATCAGTTGACGCCAGTATGGCAGGATCACTTCATCACGCGCGGTTTTCAGCATTAATGCGTGGGAGAGGTTATTGATATCTTCCGAAGTACAGGCAACGTGGATGAATTTAGAAACCGCGTGCAGTTCCGGGATCTCCGCCACTTTTTCTTTCAGGAAATACTCAACCGc                                                                                                                                                                                         >  2:88240/1‑251 (MQ=255)                                                   gCGTGGCTGGCTGACCGTGGTTACGATACAGCAGCGGGATATCGCGATACTGAACGGCGAGAGCTTTAATGCCATCAAACAGTTGACGCCAGTATGGCAGGATCACTTCATCACGCGCGGTTTTCAGCATTAATGCGTGGGAGAGGTTATGGATATCTTCCGAAGTACAGGCAAAGTGGATGAATTTAGAAACCGCGTGCAGTTCCGGGATCTCCGCCACTTTTTCTTTCAGGAAATACTCAACCGCTTTa                                                                                                                                                                                     <  2:575219/251‑1 (MQ=255)                                                          tggctgACCGTGGGTACGAGGCAGCAGCGGGATATCGCGATACTGAACGGCGAGATCTTTAATGCCATCAATCAGTTGACGCCAGTATGGCAGGATCACTTCATCACGCGCGGTTTTCAGCATTAATGCGTGGGAGAGGTTATTGATATCTTCCGAAGTACAGGCAAAGTGGATGAATTTAGAAACCGCGTGCAGTTCCGGGATCTACGCCACTTTTTCTTTCAGGAATTACTCAACCGCTTTACcgtcgg                                                                                                                                                                              >  2:482708/1‑250 (MQ=255)                                                           ggctgACCGTGGGTACGAGACAGCAGCGGGATATCGCGATACTGAACGGCGAGATCTTTAATGCCATCAATCAGTTGACGCCAGTATGGCAGGATCACTTCATCACGCGCGGTTTTCAGCATTAATGCGTGGGAGAGGTTATTGATATCTTCCGAAGTACAGGCAAAGTGGATGAATTTAGAAACCGCGTGCAGTTCCGGGATCTCCGCCACTTTTTCTTTCAGGAAATACTCAACCGCTTTAAcgtcgt                                                                                                                                                                              <  2:470291/250‑1 (MQ=255)                                                                   gTGGGTACGAGACAGCAGCGGGATATCGCGATACTGAACGGCGAGATCTTTAATGCCATCACTCAGTTGACGCCAGTATGGCAGGATCACTTCATCCCGCGCGGTTTTCAGCATTAATGCGTGGGAGAGGTTATTGATATCTTCCGAAGTACAGGCAAAGTGGATGAATTTAGAAACCGCGTGCAGTTACGGGATCTCCGCCACTTTTTCTTTCAGGAACTACTCAACCGCTTTAACGTCGTGGTTAGTgg                                                                                                                                                                     >  1:581754/1‑251 (MQ=255)                                                                      ggTACGAGACAGCAGCGGGATATCGCGATACTGAACGGCGAGATCTTTAATGCCATCAATCAGTTGACGCCAGTATGGCAGGATCACTTCATCACGCGCGGTTTTCAGCATTAATGCGTGGGAGAGGTTATTGATATCTTCCGAAGTACAGGCAAAGTGGATGAATTTAGAAACCGCGTGCAGTTCCGGGATCTCCGCCACTTTTTCTTTCAGGAAATACTCAACCGCTTTAACGTCGTGGTTAGTGGTAc                                                                                                                                                                  >  2:169188/1‑251 (MQ=255)                                                                      ggTACGAGACAGCAGCGGGATATCGCGATACTGAACGGCGAGATCTTTAATGCCATCAATCAGTTGACGCCAGTATGGCAGGATCACTTCATCACGCGCGGTTTTCAGCATTAATGCGTGGGAGAGGTTATTGATATCTTCCGAAGTACAGGCAAAGTGGATGAATTTAGAAACCGCGTGCAGTTCCGGGATCTCCGCCACTTTTTCTTTCAGGAAATACTCAACCGCTTTAACGTCGTGGTTAGTGGTAc                                                                                                                                                                  >  1:481584/1‑251 (MQ=255)                                                                            agaCAGCAGCGGGATATCGCGATACTGAACGGCGAGATCTTTAATGCCAGCAATCAGTTGACGCCAGTATGGCAGGATCACTTCATCACGCGCGGTTTTCATCATTAATGCGTGGGAGAGGTTAGTGATATCTTCCGAAGTACAGGCAAAGTGGATGAATTTAGAAACCGCGTGCATTTCCGGGATCTCCGCCACTTTTTCTTTCAGGAAATACTCAACCGCTTTAACGTCGTGGTTAGTGGTACGCTCGa                                                                                                                                                            <  1:527303/251‑1 (MQ=255)                                                                                  cagcGGGATATCGCGATACTGAACGGCGAGATCTTTAATGCCATCAATCAGTTGACGCCAGTATGGCAGGATCACTTCATCACGCGCGGTTTTCAGCATTAATGCGTGGGAGAGGTTATTGATATCTTCCGAAGTACAGGCAAAGTGGATGAATTTAGAAACCGCGTGCAGTTCCGGGATCTCCGCCACTTTTTCTTTCAGGAAATACTCAACCGCTTTAACGTCGTGGTTAGTGGTACGCTCGATAGttt                                                                                                                                                      <  2:156747/251‑1 (MQ=255)                                                                                      gggATATCGCGATACTGAACGGCGAGATCTTTAATGCCATCAATCAGTTGACGCCAGTATGGCAGGATCACTTCATCCCGCGCGGTTTTCAGCATTAATGCGTGGGAGAGGTTATTGATATCTTCCGAAGTACAGGCAAAGTGGATGAATTTAGAAACCGCGTGCAGTTCCGGGATCTCCGCCACTTTTTCTTTCAGGAAATACTCAACCGCTTTAACGTCGTGGTTAGTGGTACGCTCGATAGTTTTGAt                                                                                                                                                  >  2:580625/1‑251 (MQ=255)                                                                                         atatCGCGATCCTGAACGGCGAGATCTTTAATGCCATCAATCAGTTGCAGCCAGTATGGCAGGATCACTTCATCACGCGCGGTTTTCAGCATTAATGCGTGGGAGAGGTTATTGATATCTTCCGAAGTACAGGCAAAGTGGATGAATTTAGAAACCGCGTGCAGTTCCGGGATCTCCGCCACTTTTTCTTTCAGGAAATACTCAACCGCTTTAACGTCGTGGTTAGTGGTACGCTCGATAGTTTTGATgcg                                                                                                                                               <  2:181893/251‑1 (MQ=255)                                                                                             cgcgATACTGAACGGCGAGATCTTTAATGCCATCAATCAGTTGACGCCAGTATGGCAGGATCACTTCATCACGCGCGGTTTTCAGCATTAATGCGTGGGAGAGGTTATTGATATCTTCCGAAGTACAGGCAAAGTGGATGAATTTAGAAACCGCTTGCAGTTCCGGGATCTCCGCCCCTTTTTCTTTCAGGAAATACTCAACCGCTTTAACGTCGTGGTTAGTGGTACGCTCGATAGTTTTGATGCGcgcc                                                                                                                                           <  1:188505/251‑1 (MQ=255)                                                                                              gcgATACTGAACGGCGAGATCTTTAATGCCATCAATCAGTTGACGCCAGTATGGCAGGATCACTTCATCACGCGCGGTTTTCAGCATTAATGCGTGGGAGAGGTTATTGATATCTTCCGAAGTACAGGCAAAGTGGATGAATTTAGAAACCGCGTGCAGTTCCGGGATCTCCGCCACTTTTTCTTTCAGGAAATACTCAACCGCTTTAACGTCGTGGTTAGTGGTACGCTCGATAGTTTTGATGCGcgccg                                                                                                                                          <  1:311353/251‑1 (MQ=255)                                                                                                 aTACTGAACGGCGAGATCTTTAATGCCATCAATCAGTTGACGCCAGTATGGCAGGATCACTTCATCACGCGCGGTTTTCAGCATTAATGCGTGGGAGAGGTTATTGATATCTTCCGAAGTACAGGCAAAGTGGATGAATTTAGAAACCGCGTGCAGTTCCGGGATCTCCGCCACTTTTTCTTTCAGGAAATACTCAACCGCTTTAACGTCGTGGTTAGTGGTACGCTCGATAGTTTTGATGCGCGCCGCAt                                                                                                                                       >  2:406823/1‑251 (MQ=255)                                                                                                     ggAACGGCGAGATCTTTAATGCCATCAATCAGTTGACGCCAGTATGGCAGGATCACTTCATCACGCGCGGTTTTCAGCATTAATGCGTGGGAGAGGTTATTGATATCTTCCGAAGTACAGGCAAAGTGGATGAATTTAGAAACCGCGTGCAGTTCCGGGATCTCCGCCACTTTTTCTTTCAGGAAATACTCAACCGCTTTAACGTCGTGGTTAGTGGTACGCTCGATAGTTTTGATGCGCGCCGCAtcttc                                                                                                                                   <  1:169188/250‑1 (MQ=255)                                                                                                        aCGGCGAGATCTTTAATGCCATCAATCAGTTGACGCCAGTATGGCAGGATCACTTCATCACGCGCGGTTTTCAGCATTAATGCGTGGGAGAGGTTATTGATATCTTCCGAAGTACAGGCAAAGTGGATGAATTTAGAAACCGCGTGCAGTTCCGGGATCTCCGCCACTTTTTCTTTCAGGAAATACTCAACCGCTTTAACGTCGTGGTTAGTGGTACGCTCGATAGTTTTGATGCGCGCCGCATCttcttc                                                                                                                                <  2:152750/251‑1 (MQ=255)                                                                                                            cGAGATCTTTAATGCCATCAATCAGTTGACGCCAGTATGGCAGGATCACTTCATCACGCGCGGTTTTCAGCATTAATGCGTGGGAGAGGTTCTTGATATCTTCCGAAGTACAGGCAAAGTGGCTGAATTTAGAAACCGCGTGCAGTTCCGGGCTCTCCGCCACTTTTTCTTTCAGGAAATACTCAACCGCTTTAACGTCGTGGTTAGTGGTACGCTCGATAGTTTTGATGCGCGCCGCATCTTCTTCGCTg                                                                                                                            >  2:9767/1‑251 (MQ=255)                                                                                                                  cTTTAATGCCATCAATCAGTTGACGCCAGTATGGCAGGATCACTTCATCACGCGCGGTTTTCAGCATTAATGCGTGGGAGAGGTTATTGATATCTTCCGAAGTACAGGCAAAGTGGATGAATTTAGAAACCGCGTGCAGTTCCGGGATCTCCGCCACTTTTTCTTTCAGGAAATACTCAACCGCTTTAACGTCGTGGTTAGTGGTACGCTCGATAGTTTTGATGCGCGCCGCATCTTCTTCGCTGAAACTg                                                                                                                      >  1:8483/1‑251 (MQ=255)                                                                                                                           cATCAATCAGTTGACGCCAGTATGGCAGGATCACTTCATCACGCGCGGTTTTCAGCATTAATGCGTGGGAGAGGTTATTGATATCTTCCGAAGTACAGGCAAAGTGGATGAATTTAGAAACCGCGTGCAGTTCCGGGATCTCCGCCACTTTTTCTTTCAGGAAATACTCAACCGCTTTAACGTCGTGGTTAGTGGTACGCTCGATAGTTTTGATGCGCGCCGCATCTTCTTCGCTGAAACTGGCGACGAtt                                                                                                             >  1:155137/1‑251 (MQ=255)                                                                                                                              caatcaGTTGACGCCAGTATGGCAGGATCACTTCATCACGCGCGGTTTTCAGCATTAATGCGTGGGAGAGGTTATTGATATCTTCCGAAGTACAGGCAAAGTGGATGAATTTAGAAACCGCGTGCAGTTCCGGGATCTCCGCCACTTTTTCTTTCAGGAAATACTCAACCGCTTTAACGTCGTGGTTAGTGGTACGCTCGATAGTTTTGATGCGCGCCGCATCTTCTTCGCTGAAACTGGCGACGATTGc                                                                                                           <  1:159815/250‑1 (MQ=255)                                                                                                                                         cGCCAGTATGGCAGGATCACTTCATCACGCGCGGTTTTCAGCATTAATGCGTGGGAGAGGTTATTGATATCTTCCGAAGTACAGGCAAAGTGGATGAATTTAGAAACCGCGTGCAGTTCCGGGATCTCCGCCACTTTTTCTTTCAGGAAATACTCAACCGCTTTAACGTCGTGGTTAGTGGTACGCTCGATAGTTTTGATGCGCGCCGCATCTTCTTCGCTGAAACTGGCGACGATTGCATCAAGGTAAcc                                                                                               <  2:155137/251‑1 (MQ=255)                                                                                                                                                          cACTTCATCACGCGCGGTTTTCAGCATTAATGCGTGGGAGAGGTTATTGATATCTTCCGAAGTACAGGCAAAGTGGATGAATTTAGAAACCGCGTGCAGTTCCGGGATCTCCGCCACTTTTTCTTTCAGGAAATACTCAACCGCTTTAACGTCGTGGTTAGTGGTACGCTCGATAGTTTTGATGCGCGCCGCATCTTCTTCGCTGAAACTGGCGACGATTGCATCAAGGTAACCGATTGCGTCGgcagca                                                                               >  1:276798/1‑250 (MQ=255)                                                                                                                                                            cTTCATCACGCGCGGTTTTCAGCATTAATGCGTGGGAGAGGTTATTGCTATCTTCCGAAGTACAGGCAAAGTGGATGAATTTAGAAACCGCGTGCAGTTCCGGGATCTCCGCCACTTTTTCTTTCAGGAAATACTCAACCGCTTTAACGTCGTGGTTAGTGGTACGCTCGATAGTTTTGATGCGCGCCGCATCTTCTTCGCTGAAACTGGCGACGATTGCATCAAGGTAACCGATTGCGTCGGCAGCaaa                                                                             >  2:96253/1‑250 (MQ=255)                                                                                                                                                                    cgcgcgGCTCTCAGCATCAATGCGCCGGACAGGTTATTGATCTCTTCCGAAGTCCAGGCCACGTGGATGAATTTAGAAACCGCGCGCAGCTCCGGGATCCCCGCCACTTTTTCTTTCAGGAAATACTCAACCGCTTTCACGTCGTGGTTAGTGGTACGCTCGATAGTTTTGATGCGCGCCGCATCTTCTTCGCTGAAACTGGCGACGATTGCATCAAGGTAACCGATTGCGTCGGCAGCAAAAGCAGGAAc                                                                    >  2:576354/1‑251 (MQ=255)                                                                                                                                                                     gcgcgGTTTTCAGCATTAATGCGGGGGAGAGGTTATTGATATCTTCCGAAGTACAGGCAAAGTGGATGAATTTAGAAACCGCTTGCAGTTCCGGGATCTCCGCCACTGTTTCTTTCAGGAAATCCTCAACCGCTTGAACGTCGTGGTTAGGGGTACGCTCGATAGTTTTGAGGCGCGCCGCATCTTCTTCGCTGAACCTGGCGACGATTGCATCAAGGTAACCGATTGCGTCGGCAGCAAAAGCAGGAACt                                                                   <  2:581754/251‑1 (MQ=255)                                                                                                                                                                                aGCATTAATGCGTGGGAGAGGTTATTGATATCTTCCGAAGTACAGGCAAAGTGGATGAATTTAGAAACCGCGTGCAGTTCCGGGATCTCCGCCACTTTTTCTTTCAGGAAATACTCAACCGCTTTAACGTCGTGGTTAGTGGTACGCTCGATAGTTTTGATGCGCGCCGCATCTTCTTCGCTGAAACTGGCGACGATTGCATCAAGGTAACCGATTGCGTCGGCAGCAAAAGCAGGAACTTCCTTGATCGc                                                        >  1:222710/1‑251 (MQ=255)                                                                                                                                                                                    ttAATGCGTGGGAGAGGTTATTGATATCTTCCGAAGTACAGGCAAAGTGGATGAATTTAGAAACCGCGTGCAGTTCCGGGATCTCCGCCCCTTTTTCTTTCAGGAAATACTCAACCGCTTTAACGTCGTGGTTAGTGGTACGCTCGATAGTTTTGATGCGCGCCGCATCTTCTTCGCTGAAACTGGCGACGATTGCATCAAGGTAACCGATTGCGTCGGCAGCAAAAGCAGGAACTTCCTTGATCGCtg                                                      >  1:28734/1‑249 (MQ=255)                                                                                                                                                                                           gTGGGAGAGGTTATTGATATCTTCCGAAGTACAGGCAAAGTGGATGAATTTAGAAACCGCGTGCAGTGCCGGGATCTCCGCCACTTTTTCTTTCAGGAAATACTCAACCGCTGTAACGTCGTGGTTAGTGGTACGCTCGATAGTTTTGATGCGCGCCGCATCTTCTTCGCTGAAACTGGCGACGATTGCATCAAGGTAACCGATTGCGTCGGCAGCAAAAGCAGGAACTTCCTTGATCGCTGCGTGCGCgg                                             <  2:156639/251‑1 (MQ=255)                                                                                                                                                                                                           atatCTTCCGAAGTACAGGCAAAGTCGAGGAATTTAGAAACCGCGTGCAGCTCCGGGTGCCCCGCCTCTTTTTCTTACAGGATATACTCAACCGCTTTAACGTCGTGGTTCGTGGTACGCTCGATAGTTTTGATGCGCGCCGCTTCTTCTTCGGTGAAACTGGCGACGATTGCATCAAGGTAGCCGCTTGCGTCGGCAGTAAAAGCAGGAACTCCCTTGATCGCTGCGGTCGCGGCCAGTTTTTGCAGccg                             <  2:125069/251‑2 (MQ=255)                                                                                                                                                                                                            tatCTTCCGAAGTACAGGCAAAGTGGATGAATTTAGAAACCGCGTGCAGTTCCGGGATCTCCGCCACTTTTTCTTTCAGGAAATACTCAACCGCTTTAACGTCGTGGTTAGTGGTACGCTCGATAGTTTTGATGCGCGCCGCATCTTCTTCGCTGAAACTGGCGACGATTGCATCAAGGTAACCGATTGCGTCGGCAGCAAAAGCAGGAACTTCCTTGATCGCTGCGTGCGCGGCCAGTTTTTGCAGCCa                             >  1:323286/1‑250 (MQ=255)                                                                                                                                                                                                                      aGTACAGGCAAAGTGGATGAATTTAGAAACCGCGTGCAGTTCCGGGATCTCCGCCACTTTTTCTTTCAGGAAATACTCAACCGCTTTAACGTCGTGGTTAGTGGTACGCTCGATAGTTTTGATGCGCGCCGCATCTTCTTCGCTGAAACTGGCGACGATTGCATCAAGGTAACCGATTGCGTCGGCAGCAAAAGCAGGAACTTCCTTGATCGCTGCGTGCGCGGCCAGTTTTTGCAGCCAACGTACTTCaa                  <  1:294972/251‑1 (MQ=255)                                                                                                                                                                                                                           aGGCAAAGTGGATGAATTTAGAAACCGCGTGCAGTTCCGGGATCTCCGCCACTTTTTCTTTCAGGAAATACTCAACCGCTTTAACGTCGTGGTTAGTGGTACGCTCGATAGTTTTGATGCGCGCCGCATCTTCTTCGCTGAAACTGGCGACGATTGCATCAAGGTAACCGATTGCGTCGGCAGCAAAAGCAGGAACTTCCTTGATCGCTGCGTGCGCGGCCAGTTTTTGCAGCCAACGTACTTCAACTTGt             <  1:179154/251‑1 (MQ=255)                                                                                                                                                                                                                                    ggATGAATTTAGAAACCGCGTGCAGTTCCGGGATCTCCGCCACTTTTTCTTTCAGGAAATACTCAACCGCTTTAACGTCGTGGTTAGTGGTACGCTCGATAGTTTTGATGCGCGCCGCATCTTCTTCGCTGAAACTGGCGACGATTGCATCAAGGTAACCGATTGCGTCGGCAGCAAAAGCAGGAACTTCCTTGATCGCTGCGTGCGCGGCCAGTTTTTGCAGCCAACGTACTTCAACTTGTACACGGaa     <  2:481584/250‑1 (MQ=255)                                                                                                                                                                                                                                     gATGAATTTAGAAACCGCGAGCAGTTCCGGGATCTCCGCCACTTTTTCTTTCAGGAAATACTCAACCGCTTTAACGTCGTGGTTAGTGGTACGCTCGATAGTTTTGATGCGCGCCGCATCTTCTTCGCTGAAACTGGCGACGATTGCATCAAGGTAACCGATTGCGTCGGCAGCAAAAGCAGGAACTTCCTTGATCGCTGCGTGCGCGGCCAGTTTTTGCAGCCAACGTACTTCAACTTGTACACGGAAtt   >  2:506234/1‑251 (MQ=255)                                                                                                                                                                                                                                      aTGAATTTAGAAACCGCGTGCAGTTCCGGGATCTCCGCCACTTTTTCTTTCAGGAAATACTCAACCGCTTTAACGTCGTGGTTAGTGGTACGCTCGATAGTTTTGATGCGCGCCGCATCTTCTTCGCTGAAACTGGCGACGATTGCATCAAGGTAACCGATTGCGTCGGCAGCAAAAGCAGGAACTTCCTTGATCGCTGCGTGCGCGGCCAGTTTTTGCAGCCAACGTACTTCAACTTGTACACGGAAttt  <  1:458302/251‑1 (MQ=255)                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                    GCGCTCCATACGGTAGGCGACGTTTGCCATCTCTTTACCGATGGTTGACGGCGTGGCTGGCTGACCGTGGGTACGAGACAGCAGCGGGATATCGCGATACTGAACGGCGAGATCTTTAATGCCATCAATCAGTTGACGCCAGTATGGCAGGATCACTTCATCACGCGCGGTTTTCAGCATTAATGCGTGGGAGAGGTTATTGATATCTTCCGAAGTACAGGCAAAGTGGATGAATTCAGAAACCGCGTGCAGTTCCGGGATCTCCGCCACTTTTTCTTTCAGGAAATACTCAACCGCTTTAACGTCGTGGTTAGTGGTACGCTCGATAGTTTTGATGCGCGCCGCATCTTCTTCGCTGAAACTGGCGACGATTGCATCAAGGTAACCGATTGCGTCGGCAGCAAAAGCAGGAACTTCCTTGATCGCTGCGTGCGCGGCCAGTTTTTGCAGCCAACGTACTTCAACTTGTACACGGAATTT  >  NC_000913/1191408‑1191887

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑