Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,175,517	A→G	T31A (ACC→GCC)	lolC →	lipoprotein release complex ‑ inner membrane subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,175,517	0	A	G	100.0%	185.0 / NA	49	T31A (ACC→GCC)	lolC	lipoprotein release complex ‑ inner membrane subunit
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (32/17);  total (32/17)

AGGCGGCAAGATTGATGATAAAACATGGCAATTTAGCCGATTGATTTACGGGGGCTTTTCAGATTAGCCCTGACGATCACTTACAGTTCAGACGTTTACCCATCTTGCTTTCGCTTATATACTCGTGTCTTTGCTACAGCAACCAGACGGATTTCATGTACCAACCTGTCGCTCTATTTATTGGCCTGCGTTACATGCGTGGGCGTGCAGCGGATCGCTTCGGTCGTTTCGTCTCCTGGCTTTCTACCATCGGCATTACCCTCGGGGTGATGGCGCTGGTCACAGTATTGTCAGTGATGAACGGCTTTGAGCGCGAGCTGCAAAACAACATCCTTGGCCTGATGCCACAGGCAATTCTCTCTTCTGAGCATGGCTCTCTTAACCCGCAGCAACTCCCAGAAACGGCAGTCAAACTGGACGGCGTTAATCGCGTCGCACCTATTACTACCGGTGATGTGGTACTGCAAAGCGCGCGCAGCGTGGCG  >  NC_000913/1175272‑1175756                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                aGGCGGCAAGATTGATGATAAAACATGGCAATTTAGCCGATTGATTTACGGGGGCTTTTCAGATTAGCCCTGAAGATCACTTACAGTTCAGACGTTTACCCATCTTGCTTTCGCTTATATACTCGTGTCTTTGCTACAGCAACCAGACGGATTTCATGTACCAACCTGTCGCTCTATTTATTGGCCTGCGTTACATGCGTGGGCGTGCAGCGGATCGCTTCGGTCGTTTCGTCTCCTGGCTTTCTGCCATc                                                                                                                                                                                                                                            >  1:201224/1‑251 (MQ=255)      gcAAGATTGATGATAAAACATGGCAATTTAGCCGATTGATTTACGGGGGCTTTTCAGATTAGCCCTGACGATCACTTACAGTTCAGACGTTTACCCATCTTGCTTTCGCTTATATACTCGTGTCTTTGCTACAGCAACCAGACGGATTTCATGTACCAACCTGTCGCTCTATTTATTGGCCTGCGTTACATGCGTGGGCGTGCAGCGGATCGCTTCGGTCGTTTCGTCTCCTGGCTTTCTGCCATCGGCAt                                                                                                                                                                                                                                       <  1:554737/251‑1 (MQ=255)                       aCATGGCAATTTGGCCGATTTATTTACGAGGGCTTTTCAGATTAGCTATGACGATCACTTACAGTTCAGACGTTTACCCATCTTGCTTGCGCTTATATACACGTGTCTTTGCTACAGCAACCAGACGGATTTCATGTCCCACCCTTTCGCGCTATTTATTGGCCTGGGATACATGCGTGGGCTTGCAGCGGATCGCTTCGGGCGGTTAGTGTCCGGGCTTGCGGTCATCGGCATTACCCTCGGGGTGATgg                                                                                                                                                                                                                      <  2:105804/251‑1 (MQ=255)                          tGGCAATTTAGCCGATTGATTTACGGGGGCTTTTCAGATTAGCCCTGACGATCACTTACAGTTCAGACGTTTACCCATCTTGCTTTCGCTTATATACTCGTGTCTTTGCTACAGCAACCAGACGGATTTCATGTACCAACCTGTCGCTCTATTTATTGGCCTGCGTTACATGCGTGGGCGTGCAGCGGATCGCTTCGGTCGTTTCGTCTCCTGGCTTTCTGCCATCGGCATTACCCTCGGGGTGATGgcgc                                                                                                                                                                                                                   >  1:585034/1‑251 (MQ=255)                               aTTTAGCCGCTTGATTTACGGGGGCTTTTCAGATTAGCCCTGACGATCACTTACAGTTCAGACGTTTACCCATCTTGCTTTCGCTTATATACTCGTGTCTTTGCTACAGCAACCAGACGGATTTCATGTACCAACCTGTCGCTCTATTTATTGGCCTGCGTTACATGCGTGGGCGTGCAGCGGATCGCTTCGGTCGTTTCGTCTCCTGGCTTTCTGCCATCGGCATTACCCTCGGGGTGATGGCGCTGGTc                                                                                                                                                                                                              >  1:482251/1‑251 (MQ=255)                                   aGCCTATTGATTTACGGGAGTTTTTCAGATTAGCCCTGACGATCACTTACAGTTCAGACGTTTACCCATCTTGCTTTCGCTTATATACTCGTGTCTTTGCTACAGCAACCAGACGGATTTCATGTACCATCCTGTCGCTCTATTTATTGGCCTGCGTTACATGCGTGGGCGGGCAGCTGATCGCTTCGGTCGTTTCGTCTCCTGGCTTGCTGCCATCGGCAGTACCCTCGGGGTGATGGCGCTGGACACAg                                                                                                                                                                                                          <  2:481355/251‑1 (MQ=255)                                   aGCCGATTGATTTACGGGGGCTTGGCAGATTAGCCCTGACGATCACTTACAGTTCAGACGTTTACCCATCGTGCTTTCGCTTATATACTCGGGTCTTTGCTACAGCAACCAGTCGGATTTCATGTACCAACCTGTCGCGCTATTTATTGGCCTGCGTTACGTGCGTGGGCGTGCAGCGGATCGCTTCGGTTGTTTCGTCTCCTTGCTTTCTGCCATCGGCATTACCCTCGGGGTGATGGGGCTGGTcaca                                                                                                                                                                                                           <  1:87949/250‑1 (MQ=255)                                            attTACGGGGGCTTTTCAGATTAGCCCTGACGATCACTTACAGTTCAGACGTTTACCCATCTTGCTTTCGCTTATATACTCGTGTCTTTGCTACAGCAACCAGACGGATTTCATGTACCAACCTGTCGCTCTATTTATTGGCCTGCGTTACATGCGTGGGCGTGCAGCGGATCGCTTCGGTCGTTTCGTCTCCTGGCTTTCTGCCATCGGCATTACCCTCGGGGTGATGGCGCTGGTCACAGTATTGTc                                                                                                                                                                                                   >  1:61634/1‑249 (MQ=255)                                               tACGGGGGCTTTTCAGATTAGCCCTGACGATCACTTACAGTTCAGACGTTTACCCATCTTGCTTTCGCTTATATACTCGTGTCTTTGCTACAGCAACCAGACGGATTTCATGTACCAACCTGTCGCTCTATTTATTGGCCTGCGTTACATGCGTGGGCGTGCAGCGGATCGCTTCGGTCGTTTCGTCTCCTGGCTTTCTGCCATCGGCATTACCCTCGGGGTGATGGCGCTGGTCACAGTATTGTCAGtg                                                                                                                                                                                               >  1:339477/1‑250 (MQ=255)                                                                     ccTGACGAGCACTTACAGTTCAGACGTTTACCCATCTTGCTTTCGCTTATATACTCGTGTCTTTGCTACAGCAACCAGACGGATTTCATGTACCAACCTGTCGCTCTATTTATTGGCCTGCGTTACATGCGTGGGCGTGCAGCGGATCGCTTCGGTCGTTTCGTCTCCTTGCTTTCTGCCATCGGCATTACCCTCGGGGTGATGGCGCTGGTCACAGTATTGTCAGTGATGAACGGCTTTGAGCGCGAGCt                                                                                                                                                                        <  2:547623/251‑1 (MQ=255)                                                                                  tACAGTTCAGACGTTTACCCATCTTGCTTTCGCTTATATACTCGTGTCTTTGCTACAGCAACCAGACGGATTTCATGTACCAACCTGTCGCTCTATTTATTGGCCTGCGTTACATGCGTGGGCGTGCAGCGGATCGCTTCGGTCGTTTCGTCTCCTGGCTTTCTGCCATCGGCATTACCCTCGGGGTGATGGCGCTGGTCACAGTATTGTCAGTGATGAACGGCTTTGAGCGCGAGCTGCAAAACAACATc                                                                                                                                                           >  1:339365/1‑251 (MQ=255)                                                                                     aGTTCAGACGTTTACCCATCTTGCTTTCGCTTATATACTCGTGTCTTTGCTACAGCAACCAGACGGCTCTCATGCACAAACCTGTCGCTCTATTTATTGGCCTGCGTTACATGCGTGAGCGCGCAGCGGATCGCTTCGGTCGTTTCGTATCCTGGCTTTCTGCCATCGGCATTACCCTCGGGGTGATGGCGCTGGTCACAGTATTGTCAGTGATGAACGGCTTTGCGCGCGAGCTGCAAACCAACATCCtt                                                                                                                                                        >  1:260963/1‑251 (MQ=255)                                                                                     aGTTCAGACGTTTACCCATCTTGCTTTCGCTTATATACTCGTGTCTTTGCTACAGCAACCAGACGGATTTCATGTACCAACCTGTCGCTCCATTTATTGGCCTGCGTTACATGCGTGGGCGTGCAGCGGATCGCTTCGGTCGTTTCGTCTCCTGGCTTTCTGCCATCGGCATTACCCTCGGGGTGATGGCGCTGGTCACAGTATTGTCAGTGATGAACGGCTTTGAGCGCGAGCTGCAAAACAACATCCtt                                                                                                                                                        >  1:360575/1‑251 (MQ=255)                                                                                     aGTTCAGACGTTTACCCATCTCGCTTTCGCTTATATACTCGTGTCTTTGCTACAGCAACCAGACGGATTTCATGTACCAACCTGTCGCTCTATTTATTGGCCTGCGTTACATGCGTGGGCGTGCAGCGGAGCGCTTCGGTCGTTTCGTCTCCTGGCTTTCTGCCATCGGCATTACCCTCGGGGTGATGGCGCTGGTCACAGTATTGACAGTGATGATCGGCTTTGAGCGCGAGCTGCAAAACAACATCCtt                                                                                                                                                        >  1:418015/1‑251 (MQ=255)                                                                                           gACGTTTACCCATATTGCTTTCGCTTATATACTCGTGTATTTGCTACAGCAACCAGACGGATTTCATGTACCAACCTGTCGCTCTATTTATTGGCCTGAGTTACATGCGTGGGCGTGCAGCGGATCGCTTCGGTCGTTTCGTCTCCTGGCTTTCTGCCATCGGCATTACCCTCGGGGTGATGGCGCTGGTCACAGTATTGTCGGTGTTGAACGGCTTTGAGCGCGAGCTGCAAAACAACATCCTTGGCCTg                                                                                                                                                  >  2:265671/1‑251 (MQ=255)                                                                                                            cTTTCGCTTATATACTCGTGTCTTTGCTACAGCAACCAGACGGTTTTCATGTACCAACCTGTCGCTCTATTTATTGGCCTGCGTTACATGCGTGGGCGTGCAGCGGATCGCTTCGGTCGTTTCGTCTCCTGGCTTTCTGCCATCGGCATTACCCTCGGGGTGATGGCGCTGGTCACAGTATTGTCAGTGATGAACGGCTTTGAGCGCGAGCTGCAAAACAACATCCTTGGCCTGATGCCACAGGCAATtct                                                                                                                                 >  2:527183/1‑251 (MQ=255)                                                                                                                         tcTCGTGTCTTTGCTACAGCAACCAGACGGATTTCATGTACCAACCTGTCGCTCTATTTATTGGCCTGCGTTACATGCGTGGGCGTGCAGCGGATCGCTTCGGTCGTTTCGTCTCCTGGCTTTCTGCCATCGGCATTACCCTCGGGGTGATGGCGCTGGTCACAGTATTGTCAGTGATGAACGGCTTTGAGCGCGAGCTGCAAAACAACATCCTTGGCCTGATGCCACAGGCAATTCTCTCTTCTGAGCAt                                                                                                                    >  1:148555/2‑251 (MQ=255)                                                                                                                         aCTCGTGTCTTTGCTACAGCAACCAGACGGATTTCATGTACCAACCTGTCGCTCTATTTATTGGCCTGCGTTACATGCGTGGGCGTGCAGCGGATCGCTTCGGTCGTTTCGTCTCCTGGCTTTCTGCCATCGGCATTACCCTCGGGGTGATGGCGCTGGTCACAGTATTGTCAGTGATGAACGGCTTTGAGCGCGAGCTGCAAAACAACATCCTTGGCCTGATGCCACAGGCAATTCTCTCTTCTGAGCAt                                                                                                                    >  1:209643/1‑251 (MQ=255)                                                                                                                          cTCGTGTCTTTGCTACAGCAACCAGACGGATTTCATGTACCAACCTGTCGCTCTATTTATTGGCCTGCGTTACATGCGTGGGCGTGCAGCGGATCGCTTCGGTCGTTTCGTCTCCTGGCTTTCTGCCATCGGCATTACCCTCGGGGTGATGGCGCTGGTCACAGTATTGTCAGTGATGAACGGCTTTGAGCGCGAGCTGCAAAACAACATCCTTGGCCTGATGCCACAGGCAATTCTCTCTTCTGAGCATg                                                                                                                   >  1:219487/1‑251 (MQ=255)                                                                                                                               gtCTTTGCTACAGCAACCAGACGGATTTCATGTACCAACCTGTCGCTCTATTTATTGGCCTGCGTTACATGCGTGGGCGTGCAGCGGATCGCTTCGGTCGTTTCGTCTCCTGGCTTTCTGCCATCGGCATTACCCTCGGGGTGATGGCGCTGGTCACAGTATTGTCAGTGATGAACGGCTTTGAGCGCGAGCTGCAAAACAACATCCTTGGCCTGATGCCACAGGCAATTCTCTCTTCTGAGAATGGctct                                                                                                              >  1:374971/1‑251 (MQ=255)                                                                                                                                   ttGCTACAGCAACCAGACGGATTTCATGTACCAACCTGTCGCTCTATTTATTGGCCTGCGTTACATGCGTGGGCGTGCAGCGGATCGCTTCGGTCGTTTCGTCTCCTGGCTTTCTGCCATCGGCATTACCCTCGGGGTGATGGCGCTGGTCACAGTATTGTCAGTGATGAACGGCTTTGAGCGCGAGCTGCAAAACAACATCCTTGGCCTGATGCCACAGGCAATTCTCTCTTCTGAGCATGGctctct                                                                                                            <  1:229295/249‑1 (MQ=255)                                                                                                                                    tGCTACAGCAACCAGACGGATTTCATGTACCAACCTGTCGCTCCATTTATTGGCCTGCGTTACATGCGTGGGCGTGCAGCGGATCGCTTCGGTCGTTTCGTCTCCTGGCTTTCTGCCATCGGCATTACCCTCGGGGTGATGGCGCTGGTCACAGTATTGTCAGTGATGAACGGCTTTGAGCGCGAGCTGCAAAACAACATCCTTGGCCTGATGCCACAGGCAATTCTCTCTTCTGAGCATGTCTCTCTTa                                                                                                          <  2:360575/250‑1 (MQ=255)                                                                                                                                      cTACAGCAACCAGACGGATTTCATGTACCAACCTGTCGCTCTATTTATTGGCCTGCGTTACATGCGTGGGCGTGCAGCGGATCGCTTCGGTCGTTTCGTCTCCTGGCTTTCTGCCATCGGCATTACCCTCGGGGTGATGGCGCTGGTCACAGTATTGTCAGTGATGAACGGCTTTGAGCGCGAGCTGCAAAACAACATCCTTGGCCTGATGCca                                                                                                                                            >  1:280827/1‑214 (MQ=255)                                                                                                                                      cTACAGCAACCAGACGGATTTCATGTACCAACCTGTCGCTCTATTTATTGGCCTGCGTTACATGCGTGGGCGTGCAGCGGATCGCTTCGGTCGTTTCGTCTCCTGGCTTTCTGCCATCGGCATTACCCTCGGGGTGATGGCGCTGGTCACAGTATTGTCAGTGATGAACGGCTTTGAGCGCGAGCTGCAAAACAACATCCTTGGCCTGATGCCACAGGCAATTCTCTCTTCTGAGCATGGCTCTCTTAAcc                                                                                                       >  1:169619/1‑251 (MQ=255)                                                                                                                                      cTACAGCAACCAGACGGATTTCATGTACCAACCTGTCGCTCTATTTAGTGGCCTGCGTTACATGCGTGGGCGTGCAGCGGATCGCTTCGGTCGTTTCGTCTCCTGGCTTTCTGCCATCGGCATGACCCGCGGGGTGATGGCGCTGGTCACAGTATTGTCAGTGATGAACGGCTTTGAGCGCGAGCTGCAAAACAACATCCTTGGCCTCATGCca                                                                                                                                            <  2:280827/214‑1 (MQ=255)                                                                                                                                       tACAGCAACCAGACGGATTTCATGTACCAACCTGTCGCTCTATTTATTGGCCTGCGTTACATGCGTGGGCGTGCAGCGGATCGCTTCGGTCGTTTCGTCTCCTGGCTTTCTGCCATCGGCATTACCCTCGGGGTGATGGCGCTGGTCACAGTATTGTCAGTGATGAACGGCTTTGAGCGCGAGCTGCAAAACAACATCCTTGGCCTGCTGCCACAGGCAATTCTCTCTTCTGAGCATGGCTCTCTTAAccc                                                                                                      >  2:126067/1‑251 (MQ=255)                                                                                                                                                   aCGGATTTCATGTAACAACCTGTCGCTCTATTTATTGGCCTGCGTTACATGCGTGGGCGTGCAGCGGATCGCTTCGGTCGTTTCGTCTCCTGGCTTTCTGCCATCGGCATTACCCTCGGGGTGATGGCGCTGGTCACAGTATTGTCAGTGATGAACGGCTTTGAGCGCGAGCTGCAAAACAACATCCTTGGCCTGATGCCACAGGCAATTCTCTCTTCTGAGCATGGCTCTCTTAACCCGCAGCAACTccc                                                                                          >  2:102253/1‑251 (MQ=255)                                                                                                                                                          tCATGTGCCAACCTGTCGCTCTATTTATTGGCCGGCGTTACAGGCGTGGGCGTGCAGCGGATCGCTTCGGTCGTTTCGTCCCCTGGCTTTCTGCCATCGGCATTACCCTCGGGGTGATGGCGCTGGTCACAGTATTGTCAGTGATGAACGGCTTTGAGCGCGAGCTGCAAAACAACATCCTTGGCCTGATGCCACAGTCAATTCGCTCTTCTGAGCATGGCTCTCTTACCCCGCAGCAACTCCCAGAAAc                                                                                    <  2:401234/250‑1 (MQ=255)                                                                                                                                                           cATGTACCAACCTGTCGCTCTATTTATTGGCCTGCGTTACATGCGTGGGCGTGCAGCGGATCGCTTCGGTCGTTTCGTCTCCTGGCTTTCTGCCATCGGCATTACCCTCGGGGTGATGGCGCTGGTCACAGTATTGTCAGTGCTGAACGGCTTTGAGCGCGAGCTGCAAAACAACATCCTTGGCCTGATGCCACAGGCAATTCTCTCTTCTGAGCATGGCTCTCTTAACCCGCAGCAACTCCCAGAACCgg                                                                                  >  2:180528/1‑251 (MQ=255)                                                                                                                                                           cATGTACCAACCTGTCGCTCTATTTATTGGCCTGCGTTACATGCGTGGGCGTGCAGCGGATCGCTTCGGTCGTTTCGTCTCCTGGCTTTCTGCCATCGGCATTACCCTCGGGGTGATGGCGCTGGTCACAGTATTGTCAGTGATGAACGGCTTTGAGCGCGAGCTGCAAAACAACATCCTTGGCCTGATGCCACCGGCAATTCTCTCTTCTGAGCATGGCTCTCTTAACCCGCAGCAACACCCAGACACgg                                                                                  >  1:210308/1‑251 (MQ=255)                                                                                                                                                                  cAACCTGTCGCTCTATTTATTGGCCTGCGTTACATGCGTGGGCGTGCAGCGGATCGCTTCGGTCGTTTCGACTCCTGGCTTTCTGCCATCGGCATTACACTCGGGGTGATGGCGCTGGTCACAGTATTGTCAGTGATGAACGGCTTTGAGCGCGAGCTGCAAAACAACATCCTTGGCCTGATGCCACAGGCAATTCTCTCTTCTGAGCATGGCTCTCTTAACCCGCAGCAACTCCCAGAAACGGCAGTCa                                                                            >  1:92074/1‑250 (MQ=255)                                                                                                                                                                                atttattGGCCTGCGTTACATGCGTGGGCGTGCAGCGGATCGCTTCGGTCGTTTCGTCTCCTGGCTTTCTGCCATCGGCATTACCCTCGGGGTGATGGCGCTGGTCACAGTATTGTCAGTGATGAACGGCTTTGAGCGCGAGCTGCAAAACAACATCCTTGGCCTGATGCCACAGGCAATTCTCTCTTCTGAGCATGGCTCTCTTAACCCGCAGCAACTCCCAGAAACGGCAGTCAAACTGGACGGCGtt                                                              >  1:459914/1‑250 (MQ=255)                                                                                                                                                                                        gCCTGCGTTACATGCGTGGGCGTGCAGCGGATCGCTTCGGTCGTTTCGACTCCTGGCTTTCTGCCATCGGCATTACCCTCGGGGTGATGGCGCTGGTCACAGTATTGTCAGTGATGAACGGCTTTGAGCGCGAGCTGCAAAACAACATCCTTGGCCTGATGCCACAGGCAATTCTCTCTTCTGAGCATGGCTCTCTTAACCCGCAGCAACTCCCAGAAACGGCAGTCAAACTGGACGGCGTTAATCGCGTc                                                     >  1:73490/1‑251 (MQ=255)                                                                                                                                                                                                 ccATGCGTGGGCGTGCAGCGGATCGCTGCGGTCGTTTCGTCTCCTGGCTTTCTGCCATCGGCATTTCCCTCGGGGTGATGGCGCTGGTCACAGTATTGTCAGTGATGAACGGCTTTGAGCGCGAGCTGCAAAACAACATCCTTGGCCTGATGCCACAGGCAATTCTCTCTTCTGAGCATGGCTCTCTTAACCCGCAGCAACTCCCAGAAACGGCAGTCAAACTGGACGGCGTTAATCGCGTCGCACCTAtt                                            <  2:130977/250‑1 (MQ=255)                                                                                                                                                                                                  cATGCGTGGGCGTGCAGCGGATCGCTTCGGTCGTTTCGTCTCCTGGCTTTCTGCCATCGGCATTACCCTCGGGGTGATGGCGCTGGTCACAGTATTGTCAGTGATGAACGGCTTTGAGCGCGAGCTGCAAAACAACATCCTTGGCCTGATGCCACAGGCAATTCTCTCTTCTGAGCATGGCTCTCTTAACCCGCAGCAACTCCCAGAAACGGCAGTCAAACTGGACGGCGTTAATCGCGTCGCACCTAtt                                            >  1:11798/1‑250 (MQ=255)                                                                                                                                                                                                   aTGCGTGGGCGTGCAGCGGATCGCTTCGGTCGTTTCGTCTCCTGGCTTTCTGCCATCGGCATTACCCTCGGGGTGATGGCGCTGGTCACAGTATTGTCAGTGATGAACGGCTTTGAGCGCGAGCTGCAAAAAAACATCCTTGGCCTGATGCCACAGGCAATTCTCTCTTCTGAGCATGGCTCTCTTAACCCGCAGCAACTCCCAGAAACGGCAGTCAAACTGGACGGCGTTAATCGCGTCGCACCTATtac                                          >  2:307530/1‑251 (MQ=255)                                                                                                                                                                                                      cGTGGGCGTGCAGCGGAGCGCTGCGGTCGTTTCGTCGCCTGGCTTTCTGCCATCGGCATTACCCTCGGGGTGATGGCGCTGGTCATAGTATTGTCAGTGATGAACGGCTTTGAGCGCGAGCTGCAAACCAACATCCTTGGCCTGATGCCACAGGCAATTCTCTCTTCTGAGCATGGCTCTCTTAACCCGCAGCAACTCCCAGAAACGGCAGTCAAACTGGACGGCGTTAATCGCGTCGCACCTATtactac                                       <  2:495497/251‑1 (MQ=255)                                                                                                                                                                                                       gTGGGCGTGCAGCGGATCGCTTCGGTCGTTTCGTCTCCTGGCTTTCTGCCATCTGCATTACCCTCGGGGTGATGGCGCTGGTCACAGTATTGTCAGTGATGAACGGCTTTGAGCGCGAGCTGCAAAACAACATCCTTGGCCTGATGCCACAGGCAATTCTCTCTTCTGAGCATGGCTCTCTTAACCCGCAGCAACTCCCAGAAACGGCAGTCAAACTGGACGGCGTTAATCGCGTCGCACCTATTACTAcc                                      <  2:449353/251‑1 (MQ=255)                                                                                                                                                                                                       gTGGGCGTGCAGCGGATCGCTTCGGTCGTTTCGTCTCCTGGCTTTCTGCCATCGGCATTACCCTCGGGGTGATGGCGCTGGTCACAGTATTGTCAGTGATGAACGGCTTTGAGCGCGAGCTGCAAAACAACATCCTTGGCCTGATGCCACAGGAAATTCTCTCTTCTGAGCATGGCTCTCTTAACCCGCAGCAACTCCCAGAAACGGCAGTCAAACTGGACGGCGTTAATCGCGTCGCACCTATTACTAcc                                      >  1:260347/1‑251 (MQ=255)                                                                                                                                                                                                        tGGGCGTGCAGCGGATCGCTTCGGTCGTTTCGTCTCCTGGCTTTCTGCCATCGGCATTACCCTCGGGGTGATGGCGCTGGTCACAGTATTGTCAGTGATGAACGGCTTTGAGCGCGAGCTGCAAAACAACATCCTTGGCCTGATGCCACAGGCAATTCTCTCTTCTGAGCATGGCTCTCTTAACCCGCAGCAACTCCCAGAAACGGCAGTCAAACTGGACGGCGTTAATCGCGTCGCACCTATTACTACCg                                     >  2:461862/1‑251 (MQ=255)                                                                                                                                                                                                        tGGGCGTGCAGCGGATCGCTTCGGTCGTTTCGTCTCCTGGCTTTCTGCCATCGGCATTACCCTCGGGGTGATGGCGCTGGTCACAGTATTGTCAGTGATGAACGGCTTTGAGCGCGAGCTGCAAAACAACATCCTTGGCCTGATGCCACAGGCAATTCTCTCTTCTGAGCATGGCTCTCTTAACCCGCAGCAACTCCCAGAAACGGCAGTCAAACTGGACGGCGTTAATCGCGTCGCACCTATTACTACCg                                     >  1:357317/1‑251 (MQ=255)                                                                                                                                                                                                               gCAGCGGATCGCTTCGGTCGTTTCGTCTCCTGGCTTTCTGCCATCGGCATTACCCTCGGGGTGATGGCGCTGGTCACAGTATTGTCAGTGATGAACGGCTTTGAGCGCGAGCTGCAAAACAACATCCTTGGCCTGATGCCACAGGCAATTCTCTCTTCTGAGCATGGCTCTCTTAACCCGCAGCAACt                                                                                             >  2:296213/1‑188 (MQ=255)                                                                                                                                                                                                               gCAGCGGATCGCTTCGGTCGTTTCGTCTCCTGGCTTTCTGCCATCGGCATTACCCTCGGGGTGATGGCGCTGGTCACAGTATTGTCAGTGATGAACGGCTTTGAGCGCGAGCTGCAAAACAACATCCTTGGCCTGATGCCACAGGCAATTCTCTCTTCTGAGCATGGCTCTCTTAACCCGCAGCAACt                                                                                             <  1:296213/188‑1 (MQ=255)                                                                                                                                                                                                               gCAGCGGATCGCTGCGGTCGTTTCGTCTCCTGGCTTTCTGCCATCGGCATTACCCTCGGGGTGATGGCGCTGGTCACAGTATTGTCAGTGATGAACGGCTTTGAGCGCGAGCTGCAAAACAACATCCTTGGCCTGATGCCACAGGCAATTCTCTCTTCTGAGCATGGCTCTCTTAACCCGCAGCAACTCCCAGAAACGGCAGTCAAACTGGACGGCGTTAATCGCGTCGCACCTATTACTACCGGTGAtg                               <  2:209643/250‑1 (MQ=255)                                                                                                                                                                                                                          cTTCGGTCGTTTCGTCTCCTGGCTTTCTGCCATCGGCATTACCCTCGGGGTGATGGCGCTGGTCACAGTATTGTCAGTGATGAACGGCTTTGAGCGCGAGCTGCAAAACAACATCCTTGGCCTGATGCCACAGGCAATTCTCTCTTCTGAGCATGGCTCTCTTAACCCGCAGCAACTCCCAGAAACGGCAGTCAAACTGGACGGCGTTAATCGCGTCGCACCTATTACTACCGGTGATGTGGTACTGCaa                    >  2:16115/1‑250 (MQ=255)                                                                                                                                                                                                                                    ttCGTCTCCTGGCTTTCTGCCGTCGGCATTACCCTCGGGGTGATGGCGCTGGGCAAAGTATTGTGAGTGATGCACGGCTTTGAGCGCGAGCTGCAAAACAACATCCTTGGCCTGATGCCACAGGCAATTCTCTCTTCTGAGCATGGCTCTCTTAACCCGCAGCAACTCCCAGAAACGGCAGTCAAACTGGACGGCGTTAATCGCGGCGCACCTAGTGCAACCGGTGATGTGGTACTGCAAGGCGCGCGCa          <  2:496633/250‑1 (MQ=255)                                                                                                                                                                                                                                      cGTCTCCTGGCTTTCTGCCATCGGCATTACCCTCGGGGTGATGGCGCTGGTCACAGTATTGTCAGTGATGAACGGCTTTGAGCGCGAGCTGCAAAACAACATCCTTGGCCTGATGCCACAGGCAATTCTCTCTTCTGAGCATGGCTCTCTTAACCCGCAGCAACTCCCAGAAACGGCAGTCAAACTGGACGGCGTTAATCGCGTCGCACCGATTACTACCGGTGATGTGGTACTGCAAAGCGCGCGCAGCg       <  2:219487/251‑1 (MQ=255)                                                                                                                                                                                                                                        tctcCTGGCTTTCTGCTATCGGCATTACCCTCGGGGGGATGGAGCTGGTCACAGCATTGTCAGTGATGAACGGCTTTGAGCGCGAGCTGCAAAACAACCTCCTTGGCCTGATGCCACAGGCAATTCTCTCTTCTGAGCATGGCTCTCTTAACCCGCAGCAACTCCCAGAAACGGCAGTCAAAATGGACGGCGTTAATCGCGTCGCACCTATTACTACCGGTGATGTGGTACTGCAAAGCGCGCGCAGcatg     >  2:405883/1‑248 (MQ=255)                                                                                                                                                                                                                                           ccTGGCTTTCTGCCATCGGCATTACCCTCGGGGTGATGGCGCTGGTCACAGTATTGTCAGTGATGAACGGCTTTGAGCGCGAGCTGCAAAACAACATCCTTGGCCTGATGCCACAGGCAATTCTCTCTTCTGAGCATGGCTCTCTTAACCCGCAGCAACTCCCAGAAACGGCAGTCAAACTGGACGGCGTTAATCGCGTCGCACCTATTACTACCGGTGATGTGGTACTGCAAAGCGCGCGCAGCGTGGCg  <  1:5602/251‑1 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                AGGCGGCAAGATTGATGATAAAACATGGCAATTTAGCCGATTGATTTACGGGGGCTTTTCAGATTAGCCCTGACGATCACTTACAGTTCAGACGTTTACCCATCTTGCTTTCGCTTATATACTCGTGTCTTTGCTACAGCAACCAGACGGATTTCATGTACCAACCTGTCGCTCTATTTATTGGCCTGCGTTACATGCGTGGGCGTGCAGCGGATCGCTTCGGTCGTTTCGTCTCCTGGCTTTCTACCATCGGCATTACCCTCGGGGTGATGGCGCTGGTCACAGTATTGTCAGTGATGAACGGCTTTGAGCGCGAGCTGCAAAACAACATCCTTGGCCTGATGCCACAGGCAATTCTCTCTTCTGAGCATGGCTCTCTTAACCCGCAGCAACTCCCAGAAACGGCAGTCAAACTGGACGGCGTTAATCGCGTCGCACCTATTACTACCGGTGATGTGGTACTGCAAAGCGCGCGCAGCGTGGCG  >  NC_000913/1175272‑1175756

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑