| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,174,883 | C→T | A95T (GCG→ACG) | ycfT ← | inner membrane protein YcfT |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,174,883 | 0 | C | T | 97.1% | 104.1 / NA | 35 | A95T (GCG→ACG) | ycfT | inner membrane protein YcfT |
| Reads supporting (aligned to +/- strand): ref base C (1/0); new base T (17/16); total (19/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AGTACAAACAAGGCGAAT‑AGTGGCAGCGCCAGACGGCTAAAAATTTT‑ACATACCACGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCCGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCAT‑TGGAGGCATTGCTTAAATCGCGCTCAGGTGCCAGCCACTGGTTCAGCGCACTTAACGCCAGCCACTGCACCACGCCCCAAAGCGC‑CAGCACCCAGAAGATGTTCCAGATCCGTTTATCGAGACAA‑TTTCCCCACGGTACGCTGTCGATATAGCGGCGAATCAAATAGCCGGATATAAAGAAAAAAACCGGCATACGAAAGGGGGCAAGGTAAAGATTGAAATAGATCCAGCATTTGCTCAGGACTTCCGATAACGGATGCTGAAAAGTGGTCAGATGCGGATAAAAGGTAATGACCGAGTGATAAATCACCACCAGACAAATACA > NC_000913/1174648‑1175123 | agTACAAACAAGGCGAAT‑AGTGGCAGCGCCAGACGGCTAAAAATTTT‑ACATACCACGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCCGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCAT‑TGGAGGCATTGCTTAAATCGCGCTCAGGTGCCAGCCACTGGTTCAGCGCACTTAACGCCAGCCACTGCACCACGCCCCAAAGCGT‑CAGCACCCAGAAGAt < 1:90019/251‑1 (MQ=255) aacaaGGCGAATTAGTGG‑AGTGCCAGACGGCTAAAAATTTTTACAT‑CCACTAAAATGTAAAATAAAGCAGTCATATAACCCAAGGTGGTGGTGGGGGTGATCAACCCGTGCCGTAAATTCCCGGTGGAATCGGCCTAAGCGGCAA‑TGGATGCATTGCTTAAATCCCCCGCAGGTTCCAGCCACTGGTTCAGCGCACTTAACGCCAGCCACTGCACCACGCCCCAAAGCGT‑CAGCACCCAGAAGATGTTCCa < 1:109283/251‑1 (MQ=255) aaGGCGAAT‑AGTGGCAGCGCCAGACGGCTAAAAATTTT‑ACATACCACGAAATAGTCAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCCGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCAT‑TGGAGGCATTGCTTAAATCGCGCTCAGGTGCCAGCCACTGGTTCAGCGCACTTAACGCCAGCCACTGCACCACGCCCCAAAGCGT‑CAGCACCCAGAAGATGTTCCAGAt < 1:417061/251‑1 (MQ=255) gCGAAT‑AGTGGCAGCGCCAGACGGCTAAAAATTTT‑ACATACCACGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCCGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCAT‑TGGAGGCATTGCTTAAATCGCGCTCAGGTGCCAGCCACTGGTTCAGCGCACTTAACGCCAGCCACTGCACCACGCCCCAAAGCGT‑CAGCACCCAGAAGATGTTCCAGATCCg < 1:578987/251‑1 (MQ=255) gAAT‑AGTGGCAGCGCCCGACGGCTAAAAATTTT‑ACATACCACCAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATTATCCCGTTCAGGAACTCACCGGTGGGCTCGGCATAAGCGGCATAGGGGGGCAT‑GCCTAAATCGCGCTTCGGCGCCAGCCACACGCTCAGGCCACTTAACGCCCGTCACTGCACAACGCCCCACAG‑GCGCAGCACCCAGAAGATGTTGCAGaacacgt > 2:318606/1‑245 (MQ=255) aGTGGCAGCGCCAGACGGCTAAAAATTTT‑ACATACCACGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCCGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCAT‑TGGAGGCATTGCTTAAATCGCGCTCAGGTGCCAGCCACTGGTTCAGCGCACTTAACGCCAGCCACTGCACCACGCCCCAAAGCGT‑CAGCACCCAGAAGATGTTCCAGATCCGTTTATc < 1:325902/251‑1 (MQ=255) aaaaaTTTT‑ACATACCACGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCAAGTACAGGAACTCACCGGTGGAAACGGCATAAGCGGCAT‑TGGAGGCATTGCTTAAATCGCGCTCAGGTGCCAGCCACTGGTTCAGCGCACTTAACGCCACCCACTGCACCACGCCCCAAAGCGT‑CAGCACCCAGAAGATGTTCCAGATCCGATTATCGAGCCAA‑CTTCCCCACGGt > 1:86627/1‑250 (MQ=255) aaaTTTT‑ACATACCACGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCCGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCAT‑TGGAGGCATTGCTTAAATCGCGCTCAGGTGCCAGCCACTGGTTCAGCGCACTTAACGCCAGCCACTGCACCACGCCCCAAAGCGT‑CAGCACCCAGAAGATGTTCCAGATCCGTTTATCGAGACAA‑TTTCCCCACGGTACg < 1:527738/251‑1 (MQ=255) aTTTT‑ACATACCACGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCCGTGCAGGAACTCACCGGTGGAAACGGCATAAGCGGCAT‑TGGAGGCATTGCTTAAATCGCGCTCAGGAGCCAGCCACTGGTTCAGCGCACTTAACGCCAGCCACTGCACCACGCCCCAAAGCGT‑CCGCACCCAGAAGATGTTCCAGATCCGTTTATCGAGACAA‑ATTCCCCACGGTACGCt > 1:427548/1‑251 (MQ=255) tACCACGAAATAGACAATTAACGCATACAGATACAACAAGCTGGTGCTGGCGGTGATCATCCCGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCAT‑TGGAGGCATTGCTTAAATCGCGCTCAGGGGCCAGCCACTGGTTCAGCGCACTTAACGCCAGCCACTGCACCACGCCCCAAAGCGT‑AAGCACCCAGAAGATGTTCCAGATCCGTTTATCGAGACAA‑TTTCCCCACGGTACGCTGTCGatat > 1:446646/1‑251 (MQ=255) cACGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCCGTGCAGGAACTCACCGGGGGGATCGGCATAAGAGGCAT‑TGGAGGCCTTGCTTAAATCGCGCTCAGGTGCCAGCCACTGGTTCAGCGCACTTAACGCCAGCCCCTGCACCACGCCCCAAAGCGG‑CAGCACCCCGAAGATGTTCCAGATCCGTTTTTCGAGACAA‑TTTCCCCACGGTACGCTGTCGATATAgc > 2:400585/1‑251 (MQ=255) aCGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATAATCCCGTGCAGGCACCCCCCGGGGGAAAAAGCATAAGCAGCAT‑TTGAGGCATTGATTAAATCGCGCTCAAGTGCCAGCCACTGGTTCAGCGCACTTAACGCCCACCACTGCACCACCCCCCCAAGCGT‑CCGCACCCCGAAGATGTTCCTGATCCGGTTATCGAGACAA‑TTGCCCCACGGGACGCTGTCGATATAgcg > 1:232136/1‑251 (MQ=255) cGAAATAGACAATTAACTCATACAGATACCACAAGCTGGTGCTGGCGGTGATCAGCCCGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCAT‑TGGAGGCATTGCTTAAATCGCGCTCAGGTGCCAGCCACTGGTTCAGCGCACTTAACGCCAGCCACTGCACCACGCCCCAAAGCGT‑CAGCACCCAGAAGATGTGCCAGATCCGTTTATCGAGACAA‑TTTCCCCACGGTACGCTGTCGATATAgcgg < 2:186966/251‑1 (MQ=255) gACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGAGCATCCCGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCAT‑TGGAGGCATTGCTTAAATCGCGCTCAGGTGCCAGCCACTGGTTCAGCGCACTTAACGCCAGCCACTGCACCACGCCCCAAAGCGT‑CAGCACCCAGAAGATGTTCCAGATCCGTGTATCGAGACAA‑TTTCCCCACGGTACGCTGGCGATATAGCGGCGAATCa < 2:55510/251‑1 (MQ=255) gCATACAGATACCACAAGCTGGTGCTGGCGGAGATCATCCCGTGCAGGAACTCAACGGTGGGATCGGCATAAACGGCAT‑TGGAGGCAATGATTAAATCGCGCTCAGGTGCCAGCCAATGGGTCAGCGCCCTTTACGCCAGCCACTGCACCACGCCCCCAAGCGT‑CAGCAACCAGAAGATGATCCAGATCCGTTTATCGAGACAAATTACCCCA‑GGTAAGCTGTCTGTATAACGGCGACTCAAAtcatcgg > 1:481355/1‑244 (MQ=255) cAGATACCACAAGCTGGTGCTGGCGGTGATATTCCCGTGCCGACACTCACCGGGGGAATACGCATAAGCGGCCT‑TTGCGGCATTCCTTCAACCGCGCTCCGGAGCCAGACAAAGGTTTAGAGCACTATACGCCAGCCCCTGCACCACGCCCCAAAGCGT‑CAGCACCCACAAGATGTCCCAGATCCGTTTATCGAGACAA‑TTTACCCCCGTTACGATGTCGATATAGAGGAGAATCAAATAGCCGGAtata > 2:589188/1‑250 (MQ=255) ggTGCTGGCGGTGATCATCCCGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCAT‑TGGAGGCATTGCTTAAATCGCGCTCAGGTGCCAGCCACTGGTTCAGCGCACTTAACGCCAGCCACTGCACCACGCCCCAAAGCGT‑CAGCACCCAGAAGATGTTCCAGATCCGTTTATCGAGACAA‑TTTCCCCACGGTACGCTGTCGATATAGCGGCGAATCAAATAGCCTGATATAAAGAAAAAAACCGGc < 1:400585/250‑1 (MQ=255) ggAATCGGCATAAGCGGCAT‑TGGAGGCATTGCTTAAATCGCGCTCAGGTGCCAGCCACTGGTTCAGCGCACTTAACGCCAGCCACTGCACCACGCCCCAAAGCGT‑CAGCACCCAGAAGATGTTCCAGATCCGTTTATCGAGACAA‑TTTCCCCACGGTACGATGTCGat > 2:105627/1‑168 (MQ=255) ggAATCGGCATAAGCGGCAT‑TGGAGGCATTGCTTAAATCGCGCTCAGGTGCCAGCCACTGGTTCAGCGCACTTAACGCCAGCCACTGCACCACGCCCCAAAGCGT‑CAGCACCCAGAAGATGTTCCAGATCCGTTTATCGAGACAA‑TTTCCCCACGGTACGATGTCGat < 1:105627/168‑1 (MQ=255) gcctAAGCGGCAT‑TGGAGGCATTGCTTACATCTCGCTCAGGTGCCAGCCACTGGTTCAGCGCACTTAACGCCCGCCACTGCACCACGCCCCAAAGCGT‑CAGCACCCAGAAGATGATCCAGATCCGTTTATCGCGACAC‑TTTCCCCACGGTACGCTGTCTATCTAGCGGCGACTCAAATAGCCGGATATAAAGCAACAACCCGTCATACGCAAGGGGGCCATGTATAGCTTGCACTAGATCCAGCATTTGCt > 2:197849/4‑251 (MQ=255) cgCGGCAT‑TGGAGGAATTGCTTAAATCGCGCTCAGGAGCCAGCCACTGGTTCAGCGCACTTAACGCCAGCCACTGCACCACGCCCCAAAGCGT‑CAGCACCCAGAAGATGTTCCAGATCCGTTTATCGAGACAA‑TTTCCCCACGGTACGCTGTCGATATAGTGGCGAATCAAATAGCCGGATATAAAGAAAAACAACGACATACGAAATGGGGAACGGGAAAGATTGAAATAGATCCAGCATTTGCTCAgg > 2:17373/2‑250 (MQ=255) cAT‑TGGAGGCATTGCTTAAATCGCGCTCCGGTGCCCGCCACTGGTTCAGCGCACTTAACGCCAGCCACTGCACCACGCCCCAAAGCGT‑CAGCACCCAGAAGATGTTCCAGATCCGTTTATCGAGACAA‑TTTCCCCACGGTACGCTGTCGATATAGCGGCGAATCAAATAGCCGGATATAAAGAAAAAAACCGGCATACGAAAGGGGGCAAGGTAAAGATTGAAATAGATCCAGCATTTGCTCAGGACTTcc < 1:318606/251‑1 (MQ=255) aTTGCTTAAATCGCGCTCAGGTGCCAGCCACTGGTTCAGCGCACTTAACGCCAGCCACTGCACCACGCCCCAAAGCGT‑CAGCACCCAGAAGATGTTCCAGATCCGTTTATCGAGACAA‑TTTCCCCACGGTACGCTGTCGATATAGCGGCGAATCAAATAGCCGGATATAAAGAAAAAAACCGGCATACGAAAGGGGGCAAGGTa > 1:83941/1‑204 (MQ=255) aTTGCTTAAATCGCGCTCAGGTGCCAGCCACTGGTTCAGCGCACTTAACGCCAGCCACTGCACCACGCCCCAAAGCGT‑CAGCACCCAGAAGATGTTCCAGATCCGTTTATCGAGACAA‑TTTCCCCACGGTACGCTGTCGATATAGCGGCGAATCAAAGAGCCGGATATAAAGAAAAAAACCGGCAGACGAAAGGGGGCAAGGTa < 2:83941/204‑1 (MQ=255) gCTTAAATCGCGCTCAGGTACCAGCCACTGGTTCAGCGCACTTAACGCCAGCCACTGCACCACGCCCCAAAGCGT‑CAGCACCCAGAAGATGTTCCAGATCCGTTTATCGAGACAA‑TTTCCCCACGGTACGCTGTCGATATAGCGGCGAATCAAATAGACGGATATAAAGAAAAAAACCGGCATACGAAAGGGGGCAAGGTAAAGATTGAAATAGATCCAGCATTTGCTAAGGACTTCCGATAACGGATGCt > 2:385819/1‑251 (MQ=255) aaaTCGCGCTCAGGTGCCAGCCACTGGTTAAGCGCACTTAACGCCAGCCACTGCACCACGCCCCAAAGCGT‑CAGCACCCAGAAGATGTTCCAGATCCGTTTATCGAGACAA‑TTTCCCCACGGTACGCTGTCGATATAGCGGCGAATCAAATAGCCGGATATAAAGAAAAAAACCGGCATACGAAAGGGGGCAAGGTAAAGATTGAAATAGATCCAGCATTTGCTCAGGACTTCCGATAACGGATGCTGaa > 2:271259/1‑250 (MQ=255) gcTCAGGTGCCAGCCACTGGTTCAGCGCACTTAACGCCAGCCACTGCACCACGCCCCAAAGCGT‑CAGCACCCAGAAGATGTTCCAGATCCGTTTATCGAGACAA‑TTTCCCCACGGTACGCTGTCGATATAGCGGCGAATCAAATAGCCGGATATAAAGAAAAAAACCGGCATACGAAAGGGGGCAAGGTAAAGATTGAAATAGATCCAGCATTTGCTCAGGACTTCCGATAACGGATGCTGAAAAGTGGTc > 1:156713/1‑251 (MQ=255) cAGGTGCCAGCCACTGGTTCAGCGCACTTAACGCCAGCCACTGCACCACGCCCCAAAGCGT‑CAGCACCCAGAAGATGTTCCAGATCCGTTTATCGAGACAA‑TTTCCCCACGGTACGCTGTCGATATAGCGGAGAATCAAATAGCCGGATATAAAGAAAAAAACCGGCATACGAAAGGGGGCAAGGTAAAGATTGAAATAGATCCAGCATTTGCTCCGGACTTCCGATAACGGATGCTGCAAAGTGGTc > 2:498768/1‑248 (MQ=255) cAGGTGCCAGACACTGGTTCAGCGCACTTAACGCCAGCCACTGCACCACGCCCCGAAGCGT‑CAGCACCCAGAAGATTTTCCAGATCCGTTTATCGAGACAA‑TTTCCCCACGGTACGCTGTCGATATAGCGGCGAATCAAATATCCGGATATAAAGAAAAAAACCGGCATACGAAAGGGGGCAAGGTAAAGATTGAAATAGATCCAGCATGTGCTCAGGACTTCCGATAACGGATGCTTAAAAGTGGTCAGa < 2:588570/251‑1 (MQ=255) gccaCTGGTTCAGCGCACTTAACGCCAGCCACTGCACCACGCCCCAAAGCGT‑CAGCACCCAGAAGATGTTCCAGATCCGTTTATCGAGACAA‑TTTCCCCACGGTACGCTGTCGATATAGCGGCGAATCAAATAGCCGGATATAAAGAAAAAAACCGGCATACGAAAGGGGGCAAGGTAAAGATTGAAATAGATCCAGCATTTGCTCAGGACTTCCGATAACGGATGCTGAAAAGTGGTCAGATGCGGATa > 1:242230/1‑250 (MQ=255) cAGCGTACTGAACGCCAGCCACTGCACCACGCCCCAAAGCGT‑CAGCACCCAGAAGATGTTCCAGAACCGGTTATCGAGACAA‑TTTCCCCACGGTACGCTGTCGATATAGCGGCGAATCAAATAGCCGGATATACAGAAAAAAACCGGCATACGAAAGGGGGCAAGGTAAAGATTGAAATAGATTCAGCATTTGCTCATGACTTCCTATAACGGATGCTGAAAAGTGGTCAGATGCGGATAAAAGGTAATg < 2:449711/250‑1 (MQ=255) gcACTTAACGCCAGCCACTGCACCACGCCCCAAAGCGT‑CAGCACCCAGAAGATGTTCCAGATCCGTTTATCGAGACAA‑TTTCCCCACGGTACGCTGTCGATATAGCGGCGAATCAAATAGCCGGATATAAAGAAAAAAACCGGCATACGAAAGGGGGCAAGGTAAAGATTGAAATAGATCCAGCATTTGCTCAGGACTTCCGATAACGGATGCTGAAAAGTGGTCAGATGCGGATAAAAGGTAATGACCg > 2:458764/1‑250 (MQ=255) gCACCACGCCCCAAAGCGT‑CAGCACCCAGAAGATGTTCCAGATCCGTTTATCGAGACAA‑TTTCCCCACGGTACGCTGTCGATATAGCGGCGAATCAAATAGCCGGATATAAAGAAAAAAACCGGCATACGAAAGGGGGCAAGGTAAAGATTGAAATAGATCCAGCATTTGCTCAGGACTTCCGATAACGGATGCTGAAAAGTGGTCAGATGCGGATAAAAGGTAATGACCGAGTGATAAATCACCACCAg < 1:386350/250‑1 (MQ=255) cacGCCCCAAAGCGT‑CAGCACCCAGAAGATGTTCCAGATCCGTTTATCGAGACAA‑TTTCCCCACGGTACGCTGTCGATATAGCGGCGAATCAAATAGCCGGATATAAAGAAAAAAACCGGCATACGAAAGGGGGCAAGGTAAAGATTGAAATAGATCCAGCATTTGCTCAGGACTTCCGATAACGGATGCTGAAAAGTGGTCAGATGCGGATAAAAGGTAATGACCGAGTGATAAATCACCACCAGACaaa < 2:459857/251‑1 (MQ=255) cccAAAGCGT‑CAGCAACCAGAAGATGTTCCAGATCCGTTTATCGAGACAA‑TTTCCCCACGGTACGCTGTCGATATAGCGGCGACTCAAATAGCCGGATATACAGAAAAAAACCGGCATACGAAAGGGGGCAAGGTAAAGATTGAAATAGATCCAGCATTTGCTCAGGACTTCCGATAACGGATGCTGAAAAGGGGTCATATGCGGATAAACGGTAATGACCGAGTGATACATCACCACCAGACAAATACa > 2:78643/1‑250 (MQ=255) | AGTACAAACAAGGCGAAT‑AGTGGCAGCGCCAGACGGCTAAAAATTTT‑ACATACCACGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCCGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCAT‑TGGAGGCATTGCTTAAATCGCGCTCAGGTGCCAGCCACTGGTTCAGCGCACTTAACGCCAGCCACTGCACCACGCCCCAAAGCGC‑CAGCACCCAGAAGATGTTCCAGATCCGTTTATCGAGACAA‑TTTCCCCACGGTACGCTGTCGATATAGCGGCGAATCAAATAGCCGGATATAAAGAAAAAAACCGGCATACGAAAGGGGGCAAGGTAAAGATTGAAATAGATCCAGCATTTGCTCAGGACTTCCGATAACGGATGCTGAAAAGTGGTCAGATGCGGATAAAAGGTAATGACCGAGTGATAAATCACCACCAGACAAATACA > NC_000913/1174648‑1175123 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |