Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,494,499	C→T	intergenic (‑93/‑124)	idnD ← / → idnK	L‑idonate 5‑dehydrogenase/D‑gluconate kinase, thermosensitive

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,494,499	0	C	T	100.0%	146.4 / NA	42	intergenic (‑93/‑124)	idnD/idnK	L‑idonate 5‑dehydrogenase/D‑gluconate kinase, thermosensitive
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (15/27);  total (15/27)

TCCTGATAATAATGTAAATCGGAACCGCAAATTCCACCTCGGGTTATTTGTACTAATGTTCCATTATTATTCCAATCTATCGTCTGCTCGGTAACGGCAACAGTTTTCTTGCCCGCAACAACGCAGGACTGTGTTTTCACTTGCATAAATTCCTCACTGGTCAGGTAGACACCTCGGAAGCATTTAAGCGGTTTTAACTGTCATTATTTGTGATGAAGATCACGTCAGAAAATTGTTACATTACTATGTTACGCATAACGTGATGTGCCTTGTAATTCTTATCAGTAGAAATAAAAAAACGTGAAATTATTATGCCGCCAGGCGTAGTATCGCAGCAGGTAAGATGATTCAGGAGATTTTAAATGGCGGGTGAAAGCTTTATTTTGATGGGCGTTTCAGGGAGTGGTAAAACATTAATTGGTAGCAAGGTTGCCGCGTTATTATCTGCTAAATTTATTGATGGTGACGATCTTCATCCAG  >  NC_000913/4494261‑4494740                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                  tcctGATAATTATGTAAATCGGAACCGCAAATGCCACCTCTGGTTATTTTTACTAATGTTCCATTATTATTCCAATCTATCGTCTGCTCGGTAACGGCAATAGTTTTCTTGCCCGCAACAACGCAGGACTGTGTTTTCACTTGCATAAATTCCTCACTGGTCAGGTAGACACCTCGGAAGCATTTAAGCGGTTTTAACTGTCATTATTTGTGATGAAGATCACGTCAGAAAATTGTTATATTACTATGt                                                                                                                                                                                                                                         <  1:98681/249‑1 (MQ=255)  cctGATAATAATGTAAATCGGAACCGCAAATTCCACCTCGGGTTATTTGTACTAATGTTCCATTATTATTCCAATCTATCGTCTGCTCGGTAACGGCAACAGTTTTCTTGCCCGCAACAACGCAGGACTGTGTTTTCACTTGAATAAATTCCTCACTGGTCAGGTAGACACCTCGGAAGCATTTAAGCGGTTTTAACTGTCATTATTTGTGATGAAGATCACGTCAGAAAATTGTTATATTTCTATGTTAc                                                                                                                                                                                                                                      <  2:461336/251‑1 (MQ=255)       taataaTGGAAATCGGAACCGCAAATTCCACCTCGGGTTATTTGTACTAATGTTCCATTATTATTCCAATCTATCGTCTGCTCGGTAACGGCAACAGTTTTCTTGCCCGCAACAACGCAGGACTGTGTTTTCACTTGCATAAATTCCTCACTGGTCAGGTAGACACCTCGGAAGCATTCAAGCGGTTTTAACTGTCATTATTTGTGATGAAGATCACGTCAGAAAATTGTTATATTACTATGTTACGCAt                                                                                                                                                                                                                                  >  2:22583/1‑250 (MQ=255)           aatGTAAATCGGAACCGCAAATTCCACCTCGGGTTATTTGTACTAATGTTCCATTATTATTCCAATCTATCGTCTGCTCGGTAACGGCAACAGTTTTCTTGCCCGCAACAACGCAGGACTGTGTTTTCACTTGCATAAATTCCTCACTGGTCAGGTAGACACCTCGGAAGCATTTAAGCGGTTTTAACTGTCATTATTTGTGATGAAGATCACGTCAGAAAATTGTTATATTACTATGTTACGCATAACg                                                                                                                                                                                                                              <  1:288899/250‑1 (MQ=255)                aaaTCGGAACCGCAAATTCCACCTCGGGTTATTTGTACTAATGTTCCATTATTATTCCAATCTATCGTCTGCTCGGTAACGGCAACAGTTTTCTTGCCCGCAACAACGCAGGACTGTGTTTTCACTTGCATAAATTCCTCACTGGTCAGGTAGACACCTCGGAAGCATTTAAGCGGTTTTAACTGTCATTATTTGTGATGAAGATCACGTCAGAAAATTGTTATATTACTATGTTACGCATAACGTGAtgt                                                                                                                                                                                                                        >  1:475212/1‑251 (MQ=255)                 aaTCGGAACCGCAAATTCCACCTCGGGTTATTTTTACTAATGTTCCATTATTATTCCAATCTATCGTCTGCTCGGTAACGGCAACAGTTTTCTTGCCCGCAACAACTCAGGACTGTGTTTTCACTTGCATAAATTCCTCACTTGTCAGGTAGACACCTCGGAAGCATTTAAGCGGTTTTAACTGTCATTATTTGTGATGAAGATCACGTCAGAAAATTGTTATATTACTATGTTACGCATAACGTGAtgtg                                                                                                                                                                                                                       <  2:445218/251‑1 (MQ=255)                 aaTCGGAACCGCAAATTCCACCTCGGGTTATTTGTACTAATGTTTCATTATTATTCACATCTATCGTCTGCTCGGTAACGGCAACAGTTTTCTTGCCCGCAACAACGTAGGACTGTGTTTTCACTTGCATAAATTCCTCACTGGTCAGGTAGACACCTCGGAAGCATTTAAGCGGTTTTACCTGTCCTTATTTGTGATGACGATCACGTCAGAAAATAGTTATATTCCTATGTTACGCATAACGTGAtgtg                                                                                                                                                                                                                       >  2:422767/1‑251 (MQ=255)                    cGGAACCGCAAATTCCACCTCGGGTTATTTGTACTAATGTTCCATTATTATTCCAATCTATCGTCTGCTCGGTAACGGCAACAGTTTTCTTGCCCGCAACAACGCAGGACTGTGTTTTCACTTGCATAAATTCCTCACTGGTCAGGTAGACACCTCGGAAGCATTTAAGCGGTTTTAACTGTCATTATTTGTGATGAAGATCACGTCAGAAAATTGTTATATTACTATGTTACGCATAACGTGATGTGCCt                                                                                                                                                                                                                    >  2:13275/1‑251 (MQ=255)                    cGGAACCGCAAATTCCACCTCGGGTTATTTGTACTAATGTTCCATTATTATTCCAATCTATCGTCTGCTCGGTAACGGCAACAGTTTTCTTGCCCGCAACAACGCAGGACTGTGTTTTCACTTGCATAAATTCCTCACTGGTCAGGTAGACACCTCGGAAGCATTTAAGCGGTTTTAACTGTCATTATTTGTGATGAAGATCACGTCAGAAAATTGTTATATTACTATGTTACGCATAACGTGATGTGCCt                                                                                                                                                                                                                    <  1:172/251‑1 (MQ=255)                     ggAACCGCAAATTCCACCTCGGGTTATTTGTACTAATGTTCCATTATTATTCCAATCTATCGTCTGCTCGGTAACGGCAACAGTTTTCTTGCCCGCAACAACGCAGGACTGTGTTTTCACTTGCATAAATTCCTCACTGGTCAGGTAGACACCTCGGAAGCATTTAAGCGGTTTTAACTGTCATTATTTGTGATGAAGATCACGTCAGAAAATTGTTATATTACTATGTTGCGCATAACGTGATGTGCCtt                                                                                                                                                                                                                   <  1:185810/251‑1 (MQ=255)                     ggAACCGCAAATTCCACCTCGGGTTATTTGTACTAATGTTCCATTATTATTCCAATCTATCGTCTGCTCGGTAACGGCAACAGTTTTCTTGCCCGCAACAACGCAGGACTGTGTTTTCACTTGCATAAATTCCTCACTGGTCAGGTAGACACCTCGGAAGCATTTAAGCGGTTTTAACTGTCATTATTTGTGATGAAGATCACGTCAGAAAATTGTTATATTACTATGTTACGCATAACGTGATGTGCCtt                                                                                                                                                                                                                   >  1:179552/1‑251 (MQ=255)                     ggAACCGCAAATTACACCTCGGGTTATTTGTACTAATGTTCCATTATTATTCCAATCTATCGTCTGCTCGGTAACGGCAACAGTTTTCTTGCCCGCAACAACGCAGGCCTGTGTTTTCACTTGCATAAATTCCTCACTGGTCAGGTAGACACCTCGGAAGCATTTAAGCGGTTTTACCTGTCATTATTTGTGATGAAGATCACGTCAGAAAATTGTTATATTACTATGTTACGCATAACGTGATGTGCCtt                                                                                                                                                                                                                   >  2:341705/1‑251 (MQ=255)                         ccGCAAATTCCACCTCGGGTTATTTGTACTAATGTTCCATTATTATTCCAATCTATCGTCTGCTCGGTAACGGCAACAGTTTTCTTGCCCGCAACAACGCAGGACTGTGTTTTCACTTGCATAAATTCCTCACTGGTCAGGTAGACACCTCGGAAGCATTTAAGCGGTTTTAACTGTCATTATTTGTGATGAAGATCACGTCAGAAAATTGTTATATTACTATGTTACg                                                                                                                                                                                                                                     <  1:18329/229‑1 (MQ=255)                         ccGCAAATTCCACCTCGGGTTATTTGTACTAATGTTCCATTATTATTCCAATCTATCGTCTGCTCGGTAACGGCAACAGTTTTCTTGCCCGCAACAACGCAGGACTGTGTTTTCACTTGCATAAATTCCTCACTGGTCAGGTAGACACCTCGGAAGCATTTAAGCGGTTTTAACTGTCATTATTTGTGATGAAGATCACGTCAGAAAATTGTTATATTACTATGTTACg                                                                                                                                                                                                                                     >  2:18329/1‑229 (MQ=255)                          cGCAAATTCCACCTCGGGCTATTTGTACTAATGTTCCATTATTATTCCAATCTATCGTCTGCTCGGTAACGGAAACAGTTTTCTTGCCCGCAACAACGCAGGACTGTGTTTTCACTTGCATAAATTCCTCACTGGTCAGGTAGACACCTCGGAAGCATTTAAGCGGTTTTAACTGTCATTATTTGTGATGAAGATCACGTCAGAACATTGTTATATTACTATGTTACGCATAACGTGATGTGCCTTGTAAt                                                                                                                                                                                                              >  2:371205/1‑251 (MQ=255)                               aTTCCACCTCGGGTTATTTGTACTAATGTTCCATTATTATTCCAATCTATCGTCTGCTCGGTAACGGCAACAGTTTTCTTGCCCGCAACAACGCAGGACTGTGTTTTCACTTGCATAAATTCCTCACTGGTCAGGTAGACACCTCGGAAGCATTTAAGCGGTTTTAACTGTCATTATTTGTGATGAAGATCACGTCAGAAAATTGTTATATTACTAGGTTACGCATAACGTGATGTGCCTTGTAATTCTTa                                                                                                                                                                                                         <  2:106580/251‑1 (MQ=255)                                   cACCTCGGGTTATTTGTACTAATGTTCCATTATTATTCCAATCTATCGTCTGCTCGGTAACGGCAACAGTTTTCTTGCCCGCAACAACGCAGGACTGTGTTTTCACTTGCATAAATTCCTCACTGGTCAGGTAGACACCTCGGAAGCATTTAAGCGGTTTTAACTGTCATTATTTGTGATGAAGATCACGTCAGAAAATTGTTATATTACTATGTTACGCATAACGTGATGTGCCTTGTAATTCTTATCAg                                                                                                                                                                                                     <  2:113650/251‑1 (MQ=255)                                          ggTTATTTGTACTAATGTTCCATTATTATACCAATCTATCGTCTGCTCGGTAACGGCAACAGTTTTCTTGCCCGCAACAACGCAGGACTGTGTTTTCACTTGCATAAATTCCTCACTGGTCAGGTAGACACCTCGGAAGCATTTAAGCGGTTTTAACTGTCATTATTTGTGATGAAGATCACGTCAGAAAATTGTTATATTACTATGTTACGCATAGCGTGATGTGCCTTGTAATTCTTATCAGTAGAAAt                                                                                                                                                                                              <  2:190418/251‑1 (MQ=255)                                                  gttcgaaggtttcATTATTATTACAATCGAGCGGCTGCTCTGTAACGGCAACAGTTTTCTTGCCCTCATCAACGCAGGACTGTGTTTTCACTTGCATAAATTCCTCACTGGTCAGGTAGACACCTCGGAAGCATTGAAGCGGTTTTAACTGTCATTATTTGTGATGAAGATCACGTCAGAAAAGTTTTATATTACTATGTTACGCATAACGTGATGTGCCTTGTATTTTTTATCAGTAGAAATAAAAAAAc                                                                                                                                                                                      <  1:366501/239‑1 (MQ=255)                                                   tACTAATGTTCCATTATTCTTCCAATCTATCGTCTGCTCGGTAACGGCAACAGTTTTCTTGCCCGCAACAACGCAGGACTGTGTTTTCACTTGCATAAATTCCTCACTGGTCAGGTAGACACCTCGGAAGCATTTAAGCGGTTTTAACTGTCATTATTTGTGATGAAGATCACGTCAGAAAATTGTTATATTACTATGTTACGCATAACGTGATGTGCCTTGTAATTCTTATCAGTAGAAATAAAAAAACg                                                                                                                                                                                     >  1:435602/1‑251 (MQ=255)                                                       aaTGTTCCATTAAGATTCCAATATAACGTCGGGTCGGGATCGGCAACAGTTTTCTTGCCCGCAACAACGCAGGACTGTGTTTTCACTTGCATAAATACCTTAGTGGGCAGGTAGACACCTCGGAAGCATTTAAGCGGTTTTAACTGTCATTATTTGTGATGAAGATCACGTCAGAAAATTGTTATATTACTATGTTACGCATAAGGTGATGTGCGTTGTAAGTCTTATCAGTAGAAATGAGGAAACGTGaa                                                                                                                                                                                 <  2:548084/251‑1 (MQ=255)                                                                  attattCCAATCTATCGGCTGCTCGGTAACGGCAACAGTTTTCTTGCCCGCAACAACGCAGGACTGTGTTTTCACTTGCATAAATTCCTCACTGGTCAGGTAGACACCTCGGAAGCATTTAAGCGGTTTTAACTGTCATTATTTGTGATGAAGATCACGTCAGAAAATTGTTATATTACTATGTTACGCATAACGTGATGTGCCTTGTAATTCTTATCATTAGAAATAAAAAAACGTGAAATTATTATgcc                                                                                                                                                                      <  2:30766/251‑1 (MQ=255)                                                                         cAATCTATCGTCTGCTCGTTAACGGCAACAGTTTTCTTGCACGCACCAACGCAGGACTGTGTTTTCACTTGCATCAATTCCTCACTGGTCAGGTAGACACCTCGGAAGCATTTAAGCGGTTTTAACTGTCATTATTTGTGATGAAGATCACGTCAGAAAATTGTTATATTACTATGTTACGCATAACGTGATGTGCCTTGTAATTCTTATCAGTAGAAATAACAAAACGTGAAATTATTATGCCGCCAGGc                                                                                                                                                               >  2:541278/1‑251 (MQ=255)                                                                                  gTCTGCTCTGTAACGTCAACAGTTTTCTTGCCCGCAACAACGCAGGACTGTGTTTTCACTTGCATAAAGTCCTCACTGGTCATGTAGACACCTCGGAAGCATTTAAGCGGTTTTAACTTTCATTATTTGTGATGAAGATCACGTCAGAAAATTGTTATATTACTATGTTACGCATAACGTGATGTGCCTTGTAATTCTTATCAGTAGAAATAAAAAAGCGTTAAATTATTATGCCGCCAGGCGTAGTATCg                                                                                                                                                      <  2:81098/251‑1 (MQ=255)                                                                                    cTGCTCGGTAACGGCAACAGTTTTCTTGCCCGCAACAACGCAGGACTGTGTTTTCACTTGCATAAATTCCTCACTGGTCAGGTAGACACCTCGGAAGCATTTAAGCGGTTTTAACTGTCATTATTTGTGATGAAGATCACGTCAGAAAATTGTTATATTACTATGTTACGCATAACGTGATGTGCCTTGTAATTCTTATCAGTAGAAATAAAAAAACGTGAAATTATTATGCCGCCAGGCGTAGTATCgc                                                                                                                                                     >  2:208220/1‑250 (MQ=255)                                                                                      gCTCGGTAACGGCAACAGTTTTCTTGCCCGCAACAACGCAGGACTGTGTTTTCACTTGCATAAATTCCTCACTGGTCAGGTAGACACCTCGGAAGCATTTAAGCGGTTTTAACTGTCATTATTTGTGATGAAGATCACGTCAGAAAATTGTTATATTACTATGTTACGCATAACGTGATGTGCCTTGTAATTCTTATCAGTATAAATAAAAAAACGTGAAATTATTATGCCGCCAGGCGTAGTATCgcag                                                                                                                                                   <  2:577539/250‑1 (MQ=255)                                                                                                              tgtccGCAACAACGCAGGACTGTGGTTTCACGTGTATAAATTCCTTACCGGTCAAGTAGAAACCTCGGAAGCAATTAAACCGTTTTAACTGTCATTATTTGTGATGAAGATCTCGTCAGAAAATTGTTATATTACTATGTTACGCATAACGTGATGTGCCTTGTAATTCTTATCAGTAGAAATAAAAAAACGTGAAATTATTATGACGCCAGGCGTAGGATCGCAGCAGGTAAGATGATTCAGgagag                                                                                                                             <  2:41716/245‑2 (MQ=255)                                                                                                                    caacaaCGCAGGACTGTGTTTTCACTTGCATAAATTCCTCACTGGTCAGGTAGACACCTCGGAAGCATTTAAGCGGTTTTAACTGTCATTATTTGTGATGAAGATCACGTCAGAAAATTGTTATATTACTATGTTACGCATAACGTGATGTGCCTTGTAATTCTTATCAGTAGAAATAAAAAAACGTGAAATTATTATGCCGCCAGGCGTAGTATCGCAGCAGGTAAGATGATTCAGGAGATTTTAAATgg                                                                                                                    <  1:325272/251‑1 (MQ=255)                                                                                                                                     gtTTTCACTTGCATAAATTCCTCACTGGTCAGGTAGACACCTCGGAAGCATTTAAGCGGTTTTAACTGTCATTATTTGTGATGAAGATCACGTCAGAAAATTGTTATATTACTATGTTACGCATAACGTGATGTGCCTTGTAATTCTTATCAGTAGAAATAAAAAAACGTGAAATTATTATGCCGCCAGGCGTAGTATCGCAGCAGGTAAGATGATTCAGGAGATTTTAAATGGCGGGTGAAAGCTTTAtt                                                                                                   <  1:333416/251‑1 (MQ=255)                                                                                                                                              tGCATAAATTCCTCACTGGTCAGGTAGACACCTCGGAAGCATTTAAGCGGTTTTAACTGTCATTATTTGTGATGAAGATCACGTCAGAAAATTGTTATATTACTATGTTACGCATAACGTGATGTGCCTTGTAATTCTTATCAGTAGAAATAAAAAAACGTGAAATTATTATGCCGCCAGGCGTAGTATCGCAGCAGGTAAGATGATTCAGGAGATTTTAAATGGCGGGTGAAAGCTTTATTTTGATggg                                                                                           <  1:291783/250‑1 (MQ=255)                                                                                                                                               gCATAAATTCCTCACTGGTCAGGTAGACACCTCGGAAGCATTTAAGCGGTTTTAACTGTCATTATTTTTGATGAAGATCACGTCAGAAAATTGTTTTATTACTATGTTACGCATAACGTGATGTGCCTTGTAATTCTTATCAGTAGAAATAAAAAAACGTGAAATTATTATGCCTCCAGGCGTAGTATCGCAGCAGGTAAGATGATTCAGGAGATTTTAAATGGCGGGTGAAAGCTTTATTTTGATGGGCg                                                                                         <  1:434822/251‑1 (MQ=255)                                                                                                                                                      ttCCTCACTGGTCAGGTAGACACCTCGGAAGCATTTAAGCGGTTTTAACTGTCATTATTTGTGATGAAGATCACGTCAGAAAATTGTTATATTACTATGTTACGCATAACGTGATGTGCCTTGTAATTCTTATCAGTAGAAATAAAAAAACGTGAAATTATTATGCCGCCAGGCGTAGTATCGCAGCAGGTAAGATGATTCAGGAGATTTTAAATGGCGGGTGAAAGCTTTATTTTGATGGTCGTTTccgg                                                                                  >  2:138656/1‑248 (MQ=255)                                                                                                                                                            aCTGGTCAGGTAGACACCTCGGAAGCATTTAAGCGGTTTTAACTGTCATTATTTGTGATGAAGATCACGTCAGAAAATTGTTATATTACTATGTTACGCATAACGTGATGTGCCTTGTAATTCTTATCAGGAGAAATAAAAAAACGTGAAATTATTATGCCGCCAGGCGTAGTATCGCAGCAGGTAAGATGATTCAGGAGATTTTAAATGGCGGGTGAAAGCTTTATTTTGATGGGCGTTTCAGGGAGTgg                                                                            <  1:278308/251‑1 (MQ=255)                                                                                                                                                                  cAGGTAGACACCTCGGAAGCATTTAAGCGGTTTTAACTGTCATTATTTGTGATGAAGATCACGTCAGAAAATTGTTATATTACTATGTTACGCATAACGTGa                                                                                                                                                                                                                           <  2:151401/102‑1 (MQ=255)                                                                                                                                                                  cAGGTAGACACCTCGGAAGCATTTAAGCGGTTTTAACTGTCATTATTTGTGATGAAGATCACGTCAGAAAATTGTTATATTACTATGTTACGCATAACGTGa                                                                                                                                                                                                                           >  1:151401/1‑102 (MQ=255)                                                                                                                                                                                 gAAGCATTTCAGCGGTTTTAACTGTCATTATTTGTGATGAAGATCACGTCAGAAAATTGTTATATTACTATGTTACGCATAACGTGATGTGCCTTGTAATTCTTATCAGTAGAAATAAAAAAACGTGAAATTATTATGCCGCCAGGCGTAGTATCGCAGCAGGTAAGATGATTCAGGAGATTT‑AAATGGCGGGTGAAAGCTTTATTTTGATGGGCGTTTCAGGGAGTGGTAAAACATTAATTGGTAGCa                                                        >  2:300820/1‑249 (MQ=255)                                                                                                                                                                                 gAAGCATTTAAGCGGTTTTAACTGTCATTATTTGTGATGAAGATCACGTCAGAAAATTGTTATATTACTATGTTACGCATAACGTGATGTGCCTTGTAATTCTTATCAGTAGAAATAAAAAAACGTGAAATTATTATGCCGCCAGGCGTAGTATCGCAGCAGGTAAGATGATTCAGGAGATTT‑AAATGGCGGGTGAAAGCTTTATTTTGATGGGCGTTTCAGGGAGTGGTAAAACATTAATTGGTAGCa                                                        <  1:300820/249‑1 (MQ=255)                                                                                                                                                                                                                     atgaAGATCACGTCAGAAAATTGTTATGTTACTATGTTACGCATAACGTGATGTGCCTTGTAATTCTTATCAGTAGAAATAAAAAAACGTGAAATTATTATGCCGCCAGGCGTAGTATCGCAGCAGGTAAGATGATTCAGGAGATTTTAAATGGCGGGAGAAAGCTTTATTTTGATGGGCGTTTCAGGGAGTGGTAAAACATTAATTGGTAGCAAGGTTGCCGCGTTATTATCTTCTAAATTTATTTATgg                   >  2:510174/1‑251 (MQ=255)                                                                                                                                                                                                                     atgaAGATCACGTCAGAAAATTGTTATATTACTATGTTACGCATAACGTGATGTGCCTTGTAATTCTTATCAGTAGAAATAAAAAAACGTGAAATTATTATGCCGCCAGGCGTAGTATCGCAGCAGGTAAGATGATTCAGGAGATTTTAAATGGCGGGTGAAAGCTTTATTTTGATGGGCGTTTCAGGGAGTGGTAAAACATTAATTGGTAGCAAGGTTGCCGCGTTATTATCTGCTAAATTTATTGATgg                   <  1:426873/251‑1 (MQ=255)                                                                                                                                                                                                                             cACGTCAGAAAATTGTTATATTACTATGTTACGCATAACGTGATGTGCCTTGTAATTCTTATCAGTAGAAATAAAAAAACGTGAAATTATTATGCCGCCAGGCGTAGTATCGCAGCAGGTAAGATGATTCAGGAGATTTTAAATGGCGGGTGAAAGCTTTATTTTGATGGGCGTTTCAGGGAGTGGTAAAACATTAATTGGTAGCAAGGTTGCCGCGTTATTATCTGCTAAATTTATTGATGGTGACGATc           <  1:513439/251‑1 (MQ=255)                                                                                                                                                                                                                                  cAGAAAATTGTTATATTACTATGTTACGCATAACGTGATGTGCCTTGTAAGTCTTATCAGTAGAAATAAAAAAACGTGAAATTAGTATGCCGCCAGGCGTAGTATCGCAGCAGGTAAGATGATTCAGGAGATTTTAAATGGCGGGTGAAAGCTTTATGTTGATGGGCGTTTCAGGGAGTGTTAATACATTAATTGGTAGCAAGGTGGCCGCGTTATTATCTGCTAAATTTATTGATGGTGACGATCTTCa       <  1:262198/250‑1 (MQ=255)                                                                                                                                                                                                                                      aaaTTGTTATATTACTATGTTACGCATAACGTGATGTGCCTTGTAATTCTTATCAGTAGAAATAAAAAAACGTGAAATTATTATTCCGCCAGGCGTAGTATCGCAGCAGGTAAGATGATTCAGGAGATTTTAAATGGCGGGTGAAAGCTTTATTTTGATGGGTGTTTCAGTGAGTGGTAAAACAGTAATTGGTAGCAAGGTTGCCGCGTTATTATCTGCTAAATTTATTGATGGTGACGATCTTCATCCAg  <  1:51668/251‑1 (MQ=255)                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                  TCCTGATAATAATGTAAATCGGAACCGCAAATTCCACCTCGGGTTATTTGTACTAATGTTCCATTATTATTCCAATCTATCGTCTGCTCGGTAACGGCAACAGTTTTCTTGCCCGCAACAACGCAGGACTGTGTTTTCACTTGCATAAATTCCTCACTGGTCAGGTAGACACCTCGGAAGCATTTAAGCGGTTTTAACTGTCATTATTTGTGATGAAGATCACGTCAGAAAATTGTTACATTACTATGTTACGCATAACGTGATGTGCCTTGTAATTCTTATCAGTAGAAATAAAAAAACGTGAAATTATTATGCCGCCAGGCGTAGTATCGCAGCAGGTAAGATGATTCAGGAGATTTTAAATGGCGGGTGAAAGCTTTATTTTGATGGGCGTTTCAGGGAGTGGTAAAACATTAATTGGTAGCAAGGTTGCCGCGTTATTATCTGCTAAATTTATTGATGGTGACGATCTTCATCCAG  >  NC_000913/4494261‑4494740

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑